CN106489801A - A kind of half pierces thick lip fish artificial seedling rearing's method - Google Patents
A kind of half pierces thick lip fish artificial seedling rearing's method Download PDFInfo
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- CN106489801A CN106489801A CN201611141687.6A CN201611141687A CN106489801A CN 106489801 A CN106489801 A CN 106489801A CN 201611141687 A CN201611141687 A CN 201611141687A CN 106489801 A CN106489801 A CN 106489801A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of half pierces thick lip fish（Acrossocheilius hemispinus）Artificial seedling rearing's method, including the step such as cultivating and forming from natural water area capture original seed parent fish, parent fish rearing, antenatal reinforced cultivating, artificial induced spawning, artificial insemination, incubating oosperm, young juvenile fish cement pit artificial culture, and large size seedling seed Tu Chi.The present invention brings out first filial generation juvenile fish using the wild parent fish that cultivates, artificial breeding, then through the cultivation of 2 years, cultivates sexually matured first generation filial generation parent fish；Second filial juvenile fish is brought out using the first generation filial generation parent fish artificial breeding, artificial seedling rearing is realized.The invention provides a kind of be suitable for half pattern for piercing thick lip fry kind large-scale production.The technological break-through pierces the bottleneck that thick lip fish Wild ornamental resources are deficient and parent fish source that cause is difficult because of half; thick lip fish national level Fish Germplasm Resources protection zone is pierced for half and other wild resource reparations for having Natural Population distribution waters provide technical support and seed ensures, and can achieve half scale breeding for piercing thick lip fish and artificial cultivation industrialization.
Description
Technical field
The invention belongs to field aquaculture field, and in particular to a kind of half pierces thick lip fish artificial seedling rearing's method.
Background technology
China is made slow progress with the research work of the aspects such as exploitation in the protection of fresh water original inhabitants' characteristic fish species at present, base
Plinth is relatively weak.In recent years, many have the fresh water original inhabitants' kind for necessarily researching and developing value due to original producton location ecological environment day
Benefit deteriorates, and fishery output is greatly reduced；Add the research of the aspects such as its artificial breeding technology, nutritional need and cultural technique still
Do not break through, supply falls short of demand for the marketable fish on market, the phenomenon that is draind the pond to get all the fish by excessively capturing of ordering about of economic interests etc. is even more prohibited not repeatly
Only, the serious exhaustion of population quantity and quality of these indigenous kinds, Wild ornamental resources is caused to there is the risk of extinction.In view of
This, it is necessary to the research work such as protection of resources, seed expanding propagation and cultivation exploitation are carried out to the distinctive fresh water original inhabitants kind of China, with
The recovery and continuity of its Wild ornamental resources is realized, the market demand is met.
Half pierces thick lip fish is《Nanpu small stream half pierces thick lip fish national level Fish Germplasm Resources protection zone》（The Ministry of Agriculture batch in 2015
Quasi- foundation）With《Songxi thickness lip fish national level Fish Germplasm Resources protection zone》（Ministry of Agriculture's approval in 2016 is set up）Main protection
Species are Fujian Zhejiang and Jiangxi one with common edible fish, deep liked by common people, wide market, with the important ecological value
And nutritive value.
For various reasons, few about the half correlational study data for piercing thick lip fish, its fundamental biological knowledge, breed biology
The research of the aspects such as, aquaculture organism, threpsology and disease Control Technology is substantially at blank；Artificial breeding is rarely seen Liu Li
Beautiful etc.（2010）Thick lip fish parent is pierced using raise and train wild half, has carried out its artificial breeding test in 2008～2009 years,
Obtain a small amount of seed.In consideration of it, present invention employs new oxytocic drug compatibility, it is achieved that good spawning effect；In solution
Determined biological feed stable sufficient provisioning technique, seed and parent fish Cultivating techniques after, define stable seed rearing skill
Art, it is achieved that half pierces the artificial seedling rearing of thick lip fish, is the thickness lip fry kind large-scale production of half thorn and its protection of germ plasm resource
Solid foundation has been established with cultivation development and application.
Content of the invention
It is an object of the invention to, the present situation that thick lip fingerling Seedling Cultivating techniques lack is pierced for above-mentioned half, there is provided Yi Zhongban
The thick lip fish artificial seedling rearing's method of thorn.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of half pierces thick lip fish artificial seedling rearing's method, and which comprises the following steps that：
（1）Parent culture：
（a）Daily cultivation：Thick for half thorn lip fish wild type is put in a suitable place to breed respectively and carry out in Yu Tuchi acclimatization culture, breeding density 3～5
Tail/m2, separately cover and support silver carp Aristichthys nobiliss to regulate and control water quality；Cultivated using the artifical compound feed of protein content 33%～36%, day throws
Feed 2 times, day feeding volume for fish body weight 0.5%～1%；
Further, the Tu Chi be area in 2000 m2Within, 1.0～1.5 m of the depth of water, it is ensured that miniflow water；
（b）Antenatal reinforced cultivating：In season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, proceed to cement pit
Reinforced cultivating is carried out, each cement pit throws in 200～300 tail of parent fish, keep 3～5 h/d of pond bath；10 before artificial induced spawning
～15 d guarantee 8 more than h/d of flushing period, to promote parent fish gonad development；Hand fit using protein content 36%～40%
Feedstuff and greenfeed are cultivated, feed mixed feed day 2～3 times, day feeding volume for fish body weight 1%～2%, greenfeed is every other day
Feed 1 time；
Further, the cement pit is 40 m of area2～60 m2, the rectangle cement pit of 0.5～0.8 m of the depth of water, and be equipped with
1.5 one, kw water pumps, keep miniflow water, intake；
（2）Artificial induced spawning：
Selecting the healthy and strong individuality without wound, gonadal maturation from the parent fish of reinforced cultivating carries out artificial induced spawning test：Hasten parturition sharp
Element adopts luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid DOM (DOM)+chorionic-gonadotropin hormone (HCG)
Mix preparation, 2 pin injection systems；Oxytocic drug injected by 1st pin：3 mg LRH-A2+4 mg DOM+100 IU HCG)；2nd pin
Injection oxytocic drug：5 mg LRH-A2+5 mg DOM+1000 IU HCG)；10～12 h of needle gage, milter do not inject the 1st pin,
2nd injection amount for raun 1/2；Under 23 DEG C～27 DEG C water temperature conditions, Effect time is 10～14 h；Female-male proportion be 3～
5:1；
（3）Artificial insemination：Artificial insemination adopts dry method insemination method；
（4）Artificial incubation：
Germ cell after by imbibition is evenly arranged on hatching cloth ovum frame, under 25 DEG C～27 DEG C hatching water temperature conditions, young
Fish is about through 58～60 h membranes；3 age in days prolarvas artificially should not startle；Guarantee to hatch in hatching process water body dissolved oxygen in 5 mg/
More than l；Hatching pattern is using micro- Lotic hatching or the inflation hatching of miniflow water；
（5）Seed early stage is cultivated：
（a）Open-mouthed bait is cultivated：With 2000 m of area2Within, the Tu Chi of 0.6 ~ 0.8 m of the depth of water is bait culture pond, is starting
What front 8～12 d of artificial induced spawning work carried out bait culture pond levels work；7～9 d after cleaning up the pond, treats that drug toxicity disappears,
New water is injected to 0.5～0.6 m of the depth of water；Every 667 m280～100 kg of fertilizer for fermenting is applied, to cultivate dynamic plant of swimming
Thing；
（b）Larvae cultivation：The zooplankton that 80 mesh sieve in above-mentioned bait culture pond, 10～25 ages in days are fed before 10 age in days of prelarva
The zooplankton with 60 mesh sieved then is fed, and 20 ages in days start the artifical compound feed for taming and dociling food protein content 40%～45%；Young
Cultivation of the fish through 39～51 d, you can obtain specification up to the juvenile fish of 3 more than cm, the juvenile fish is summer flower seed；
（6）First filial generation large size seedling seed is cultivated：
The pool under the first filial generation juvenile fish clear water of 3～5 cm of specification is proceeded to Tu Chi carries out large size seedling seed cultivation and forms；Day feeds
Mixed feed 2～3 times, day feeding volume for fish body weight 3%～5%, heavy property of the mixed feed for protein content 38%～42%
Particulate material；When most of first filial generation seed size is up to 10 cm, seed harvesting sizing screening, the seed of 10 below cm are continued
Tu Chi cultivations are stayed in, the seed of 10 more than cm proceeds to other Tu Chi, carries out standby parent culture；
Further, the native pool area is in 2000 m2Within, 0.8～1.0 cm of the depth of water, miniflow water are intake with 60～80 mesh
Bolting silk cloth is filtered；
（7）First filial generation parent forms and cultivation：
（a）First filial generation standby parent form：Feed mixed feed 2～3 times day, day feeding volume for fish body weight 2%～3%, this is matched somebody with somebody
Close heavy property particulate material of the feedstuff for protein content 36%～40%；When most of first filial generation standby parent specification is up to 15 cm, will
Its harvesting carries out second sizing screening, and the standby parent for screening 15 more than cm proceeds to other Tu Chi, carries out first filial generation parent
Cultivate；
Further, parent culture pond is step（6）Described in Tu Chi；
（b）First filial generation parent culture：The same step of breeding method（1）；
（8）The realization of artificial seedling rearing:Using step（1）Operational approach obtains gonad fully-developed first filial generation parent, repeats
Step（2）To step（4）, second filial seed is obtained, artificial seedling rearing is realized.
Step（3）The concrete operation step of described dry method insemination method is：Raun abdominal part moisture to effector phase is wiped
Dry, in the container for gently pressing its abdominal part that ovum is clamp-oned dry from gonopore；The essence of extrusion is sucked from milter gonopore with suction pipe simultaneously
Liquid, with pouring in freshly extruded ovum after 0.7% normal saline dilution of 1～2 times of volume pH 7.0, then is gently mixed with feather mixed
After even 2～3 min, add clear water to clean germ cell and go to glue, then hatched.
The beneficial effects of the present invention is：
Half pierces thick lip fish has the important ecological value and nutritive value, in recent years as original producton location ecological environment goes from bad to worse,
The serious exhaustion of the population quantity and quality of its Wild ornamental resources.Present invention employs new oxytocic drug compatibility, it is achieved that good
Good spawning effect；Solve the stable sufficient provisioning technique of biological feed（A kind of new zooplankton harvester, patent
Number：ZL201520003941.0）, seed and parent fish Cultivating techniques, define stable rearing of fingerling, at home outside
The secondary artificial seedling rearing for achieving half thorn thickness lip fish, breaches because of half parent fish for piercing thick lip fish Wild ornamental resources scarcity and causing
The difficult bottleneck in source, be half thorn thickness lip fish national level Fish Germplasm Resources protection zone and other have Natural Population distribution waters
Wild resource reparation provides technical support and seed ensures, and can achieve half scale breeding and artificial cultivation for piercing thick lip fish
Industrialization.
Specific embodiment
Embodiment 1
4～June in 2015, the million emerging fingerlings cultivation company limited in Shunchang, Fujian Province, with the inventive method, using cultivated
Half pierces thick lip fish wild type, has successfully carried out its artificial breeding.Hasten parturition altogether 6 batches, total 227 tail of raun of hastening parturition is hastened parturition
187 tails of success, the mean spawning rate is up to 82.4 %；Obtain 16.55 ten thousand, ovum, 11.8 ten thousand, germ cell, average rate of fertilization 71.4
%；79660 tail of hatching spray Seedling, 67.4 % of average percentage hatch rate.Through the cultivation of 40～51 d, 3.37 cm of average size is obtained altogether
Thick 62630 tail of lip fish summer flower seed of half thorn, seed rearing average viability reaches 78.6 %.Which specifically breeds step：
（1）Parent culture：
（a）Daily cultivation：Thick for half thorn lip fish wild type is put in a suitable place to breed respectively and carry out in Yu Tuchi acclimatization culture, 3 tail of breeding density/
m2, separately cover and support silver carp Aristichthys nobiliss to regulate and control water quality；Cultivated using the artifical compound feed of protein content 33%, fed day 2 times,
Day feeding volume for fish body weight 0.5%；
Further, the Tu Chi is that area is 1000 m2, 1.0 m of the depth of water, miniflow water；
（b）Antenatal reinforced cultivating：In season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, proceed to cement pit
Reinforced cultivating is carried out, each cement pit throws in 200 tail of parent fish, keep 3 h/d of pond bath；Before artificial induced spawning, 10 d guarantee to rush
8 h/d of water time, to promote parent fish gonad development；Cultivated using the artifical compound feed and greenfeed of protein content 36%,
Feed mixed feed day 2 times, day feeding volume for fish body weight 1%, greenfeed feeds 1 time every other day；
Further, the cement pit is 40 m of area2, the rectangle cement pit of 0.5 m of the depth of water, and it is equipped with 1.5 kw water pumps one
Platform, keeps miniflow water, intakes；
（2）Artificial induced spawning：
Selecting the healthy and strong individuality without wound, gonadal maturation from the parent fish of reinforced cultivating carries out artificial induced spawning test：Hasten parturition sharp
Element adopts luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid DOM (DOM)+chorionic-gonadotropin hormone (HCG)
Mix preparation, 2 pin injection systems；Oxytocic drug injected by 1st pin：3 mg LRH-A2+4 mg DOM+100 IU HCG)；2nd pin
Injection oxytocic drug：5 mg LRH-A2+5 mg DOM+1000 IU HCG)；12 h of needle gage, milter do not inject the 1st pin, the 2nd pin
Dosage for raun 1/2；Under 23 DEG C～25 DEG C of water temperature condition, the hormone effect time is 12～14 h；Female-male proportion is 3:
1；
（3）Artificial insemination：Artificial insemination adopts dry method insemination method；
（4）Artificial incubation：
Germ cell after by imbibition is evenly arranged on hatching cloth ovum frame, and under 25 DEG C of hatching water temperature conditions, prelarva about passes through
60 h membranes；3 age in days prolarvas artificially should not startle；Guarantee to hatch in hatching process water body dissolved oxygen in 5 mg/l；Hatching pattern
Using micro- Lotic hatching；
（5）Seed early stage is cultivated：
（a）Open-mouthed bait is cultivated：With 1000 m of area2, the Tu Chi of 0.6 m of the depth of water is bait culture pond, is starting artificial induced spawning
What front 8 d of work carried out bait culture pond levels work；7 d after cleaning up the pond, treats that drug toxicity disappears, and injects new water to the depth of water
0.5 m；Every 667 m280 kg of fertilizer for fermenting is applied, to cultivate plankton；
（b）Larvae cultivation：The zooplankton that 80 mesh sieve in above-mentioned bait culture pond, 10～25 ages in days are fed before 10 age in days of prelarva
Then sieved with 60 mesh, 20 ages in days start the artifical compound feed for taming and dociling food protein content 40%, heavy property particulate material fed by the feedstuff；Young
Cultivation of the fish through 40～51 d, you can obtain specification up to the juvenile fish of 3 more than cm, the juvenile fish is summer flower seed；
（6）First filial generation large size seedling seed is cultivated：
The pool under the first filial generation juvenile fish clear water of 3 cm of specification is proceeded to Tu Chi carries out large size seedling seed cultivation and forms；Day feeds cooperation
Feedstuff 2 times, day feeding volume for fish body weight 3%, heavy property particulate material of the mixed feed for protein content 38%；Treat most of son
When generation seed size is up to 10 cm, seed harvesting sizing screening, the seed of 10 below cm are continued to cultivate in Tu Chi, 10 cm
Above seed proceeds to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 1000 m2, 0.8 m of the depth of water, holding miniflow water are intake with 60 mesh sieve silk mistakes
Filter；
（7）First filial generation parent forms and cultivation：
（a）First filial generation standby parent form：Feed mixed feed 2 times, day feeding volume for fish body weight 2% day, the mixed feed is
The heavy property particulate material of protein content 36%；When most of first filial generation standby parent specification is up to 15 cm, its harvesting is carried out
Secondary grading is screened, and the standby parent for screening 15 more than cm proceeds to other Tu Chi, carries out first filial generation parent culture；
Further, parent culture pond is step（6）Described in Tu Chi；
（b）First filial generation parent culture：The same step of breeding method（1）；
Step（3）The concrete operation step of described dry method insemination method is：Raun abdominal part moisture to effector phase is dried, gently
In the container for pressing its abdominal part that ovum is clamp-oned dry from gonopore；Suck the seminal fluid of extrusion simultaneously with suction pipe from milter gonopore, use 1
Pour in freshly extruded ovum after 0.7% normal saline dilution of times volume pH 7.0, then 2 min of mixing are gently mixed with feather
Afterwards, add clear water to clean germ cell to go to glue, then hatched.
Embodiment 2
5～June in 2016, the million emerging fingerlings cultivation company limited in Shunchang, Fujian Province are wild using the thick lip fish of half thorn that cultivates
Parent, has carried out its artificial breeding work with the inventive method.Hasten parturition altogether 5 batches, 452 tail of raun of hastening parturition, spawning rate
77.2%；Obtain 19.33 ten thousand, ovum, 150,000, germ cell, rate of fertilization 77.6%；Obtain 11.8 ten thousand tail of spray Seedling, incubation rate
78.7%.Through the cultivation of 39～48 d, half thorn thickness lip fish summer flower seed, 77500 tail of 3.16 cm of average size, Seedling is obtained altogether
Plant cultivation average viability and reach 65.7 %.
Which comprises the following steps that：
（1）Parent culture：
（a）Daily cultivation：Thick for half thorn lip fish wild type is put in a suitable place to breed respectively and carry out in Yu Tuchi acclimatization culture, 4 tail of breeding density/
m2, separately cover and support silver carp Aristichthys nobiliss to regulate and control water quality；Cultivated using the artifical compound feed of protein content 35%, fed 3 day, day
Feeding volume for fish body weight 0.8%；
Further, the native pool area is 2000 m2, 1.2 m of the depth of water, holding miniflow water；
（b）Antenatal reinforced cultivating：In season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, proceed to cement pit
Reinforced cultivating is carried out, each cement pit throws in 250 tail of parent fish, keep 4 h/d of pond bath；Before artificial induced spawning, 12 d guarantee to rush
10 h/d of water time, to promote parent fish gonad development；Trained using the artifical compound feed and greenfeed of protein content 38%
Educate, feed mixed feed day 3 times, day feeding volume for fish body weight 1.5%, greenfeed feeds 1 time every other day；
Further, the cement pit is 50 m of area2, the rectangle cement pit of 0.6 m of the depth of water, and it is equipped with 1.5 kw water pumps one
Platform, keeps miniflow water, intakes；
（2）Artificial induced spawning：
Selecting the healthy and strong individuality without wound, gonadal maturation from the parent fish of reinforced cultivating carries out artificial induced spawning test：Hasten parturition sharp
Element adopts luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid DOM (DOM)+chorionic-gonadotropin hormone (HCG)
Mix preparation, 2 pin injection systems；Oxytocic drug injected by 1st pin：3 mg LRH-A2+4 mg DOM+100 IU HCG)；2nd pin
Injection oxytocic drug：5 mg LRH-A2+5 mg DOM+1000 IU HCG)；11 h of needle gage, milter do not inject the 1st pin, the 2nd pin
Dosage for raun 1/2；Under 25 DEG C～27 DEG C of water temperature condition, the hormone effect time is 10～12 h.Female-male proportion is 4:
1；
（3）Artificial insemination：Artificial insemination adopts dry method insemination method；
（4）Artificial incubation：
Germ cell after by imbibition is evenly arranged on hatching cloth ovum frame, and under 26 DEG C of hatching water temperature conditions, prelarva about passes through
59 h membranes；3 age in days prolarvas artificially should not startle；Guarantee to hatch in hatching process water body dissolved oxygen in 5 more than mg/l；Hatching
Mode is using micro- Lotic hatching or the inflation hatching of miniflow water；
（5）Seed early stage is cultivated：
（a）Open-mouthed bait is cultivated：With 2000 m of area2, the Tu Chi of 0.7 m of the depth of water is bait culture pond, is starting artificial induced spawning
What front 10 d of work carried out bait culture pond levels work；8 d after cleaning up the pond, treats that drug toxicity disappears, and injects new water to the depth of water
0.6 m；Every 667 m290 kg of fertilizer for fermenting is applied, to cultivate plankton；
（b）Larvae cultivation：The zooplankton that 80 mesh sieve in above-mentioned bait culture pond, 10～25 ages in days are fed before 10 age in days of prelarva
Then sieved with 60 mesh, 20 ages in days start the artifical compound feed for taming and dociling food protein content 43%, heavy property particulate material fed by the feedstuff；Young
Cultivation of the fish through 39～50 d, you can obtain specification up to the juvenile fish of 3 more than cm, the juvenile fish is summer flower seed；
（6）First filial generation large size seedling seed is cultivated：
The pool under the first filial generation juvenile fish clear water of specification 4cm is proceeded to Tu Chi carries out large size seedling seed cultivation and forms；Day feeds cooperation
Feedstuff 2 times, day feeding volume for fish body weight 4%, heavy property particulate material of the mixed feed for protein content 40%；Treat most of son
When generation seed size is up to 10 cm, seed harvesting sizing screening, the seed of 10 below cm are continued to cultivate in Tu Chi, 10 cm
Above seed proceeds to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 2000 m2, 0.9 m of the depth of water, holding miniflow water are intake；
（7）First filial generation parent forms and cultivation：
（a）First filial generation standby parent form：Feed mixed feed 3 times, day feeding volume for fish body weight 2.5%, the mixed feed day
Heavy property particulate material for protein content 38%；When most of first filial generation standby parent specification is up to 15 cm, its harvesting is carried out
Second sizing screening, the standby parent for screening 15 more than cm proceed to other Tu Chi, carry out first filial generation parent culture；
Further, parent culture pond is step（6）Described in Tu Chi；
（b）First filial generation parent culture：The same step of breeding method（1）；
Step（3）The concrete operation step of described dry method insemination method is：Raun abdominal part moisture to effector phase is dried, gently
In the container for pressing its abdominal part that ovum is clamp-oned dry from gonopore；Suck the seminal fluid of extrusion simultaneously with suction pipe from milter gonopore, use 2
Pour in freshly extruded ovum after 0.7% normal saline dilution of times volume pH 7.0, then 2.5 min of mixing are gently mixed with feather
Afterwards, add clear water to clean germ cell to go to glue, then hatched.
Embodiment 3
On June 21st, 2016, using the parent from numerous first filial generation seed rearing of 2014, by artificial induced spawning, squeeze ovum
Insemination, obtains first filial generation parent spawning 31000,7600, germ cell, rate of fertilization 24.5%；2300 tail of spray Seedling is obtained, is incubated
Rate 30.3%, it is achieved that half pierces the artificial seedling rearing of thick lip fish.Which specifically breeds step：
（1）Parent culture：
（a）Daily cultivation：Thick for half thorn lip fish wild type is put in a suitable place to breed respectively and carry out in Yu Tuchi acclimatization culture, 5 tail of breeding density/
m2, separately cover and support silver carp Aristichthys nobiliss to regulate and control water quality；Cultivated using the artifical compound feed of protein content 36%, fed 3 day, day
Feeding volume for fish body weight 1%；
Further, the native pool area is 1000 m2, 1.5 m of the depth of water, holding miniflow water；
（b）Antenatal reinforced cultivating：In season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, proceed to cement pit
Reinforced cultivating is carried out, each cement pit throws in 300 tail of parent fish, keep 5 h/d of pond bath；Before artificial induced spawning, 15 d guarantee to rush
10 h/d of water time, to promote parent fish gonad development；Trained using the artifical compound feed and greenfeed of protein content 40%
Educate, feed mixed feed day 3 times, day feeding volume for fish body weight 2%, greenfeed feeds 1 time every other day；
Further, the cement pit is 60 m of area2, the rectangle cement pit of 0.8 m of the depth of water, and it is equipped with 1.5 kw water pumps one
Platform, keeps miniflow water, intakes；
（2）Artificial induced spawning：
Selecting the healthy and strong individuality without wound, gonadal maturation from the parent fish of reinforced cultivating carries out artificial induced spawning test：Hasten parturition sharp
Element adopts luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid DOM (DOM)+chorionic-gonadotropin hormone (HCG)
Mix preparation, 2 pin injection systems；Oxytocic drug injected by 1st pin：3 mg LRH-A2+4 mg DOM+100 IU HCG)；2nd pin
Injection oxytocic drug：5 mg LRH-A2+5 mg DOM+1000 IU HCG)；10 h of needle gage, milter do not inject the 1st pin, the 2nd pin
Dosage for raun 1/2；Under 25 DEG C～27 DEG C of water temperature condition, the hormone effect time is 10～12 h；Female-male proportion is 5:
1；
（3）Artificial insemination：Artificial insemination adopts dry method insemination method；
（4）Artificial incubation：
Germ cell after by imbibition is evenly arranged on hatching cloth ovum frame, and under 27 DEG C of hatching water temperature conditions, prelarva about passes through
58 h membranes；3 age in days prolarvas artificially should not startle；Guarantee to hatch in hatching process water body dissolved oxygen in 5 more than mg/l；Hatching
Mode adopts micro- Lotic hatching；
（5）Seed early stage is cultivated：
（a）Open-mouthed bait is cultivated：With 2000 m of area2, the pond bait culture pond the most of 0.8 m of the depth of water, start manually urge
What front 12 d for producing work carried out bait culture pond levels work；9 d after cleaning up the pond, treats that drug toxicity disappears, and injects new water to water
Deep 0.6 m；Every 667 m2100 kg of fertilizer for fermenting is applied, to cultivate plankton；
（b）Larvae cultivation：The zooplankton that 80 mesh sieve in above-mentioned bait culture pond, 10～25 ages in days are fed before 10 age in days of prelarva
Then sieved with 60 mesh, 20 ages in days start the artifical compound feed for taming and dociling food protein content 45%, heavy property particulate material fed by the feedstuff；Young
Cultivation of the fish through 39～48 d, you can obtain specification up to the juvenile fish of 3 more than cm, the juvenile fish is summer flower seed；
（6）First filial generation large size seedling seed is cultivated：
The pool under the first filial generation juvenile fish clear water of 3 cm of specification is proceeded to Tu Chi carries out large size seedling seed cultivation and forms；Day feeds cooperation
Feedstuff 3 times, day feeding volume for fish body weight 5%, heavy property particulate material of the mixed feed for protein content 42%；Treat most of son
When generation seed size is up to 10 cm, seed harvesting sizing screening, the seed of 10 below cm are continued to cultivate in Tu Chi, 10 cm
Above seed proceeds to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 2000 m2, 1.0 m of the depth of water, holding miniflow water are intake with 80 mesh sieve silk mistakes
Filter；
（7）First filial generation parent forms and cultivation：
（a）First filial generation standby parent form：Feed mixed feed 3 times, day feeding volume for fish body weight 3% day, the mixed feed is
The heavy property particulate material of protein content 40%；When most of first filial generation standby parent specification is up to 15 cm, its harvesting is carried out
Secondary grading is screened, and the standby parent for screening 15 more than cm proceeds to other Tu Chi, carries out first filial generation parent culture；
Further, parent culture pond is step（6）Described in Tu Chi；
（b）First filial generation parent culture：The same step of breeding method（1）；
（8）The realization of artificial seedling rearing：Using step（1）Operational approach obtains gonad fully-developed first filial generation parent, repeats
Step（2）To step（4）, second filial seed is obtained, artificial seedling rearing is realized.
Step（3）The concrete operation step of described dry method insemination method is：Raun abdominal part moisture to effector phase is wiped
Dry, in the container for gently pressing its abdominal part that ovum is clamp-oned dry from gonopore；The essence of extrusion is sucked from milter gonopore with suction pipe simultaneously
Liquid, with pouring in freshly extruded ovum after 0.7% normal saline dilution of 2 times of volumes pH 7.0, then is gently mixed mixing 3 with feather
After min, add clear water to clean germ cell and go to glue, then hatched.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes that is done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (2)
1. a kind of half thick lip fish artificial seedling rearing's method is pierced, it is characterised in which comprises the following steps that：
（1）Parent culture：
（a）Daily cultivation：Thick for half thorn lip fish wild type is put in a suitable place to breed respectively and carry out in Yu Tuchi acclimatization culture, breeding density 3～5
Tail/m2, separately cover and support silver carp Aristichthys nobiliss to regulate and control water quality；Cultivated using the artifical compound feed of protein content 33%～36%, day throws
Feed 2 times, day feeding volume for fish body weight 0.5%～1%；
Further, the native pool area is in 2000 m2Within, 1.0～1.5 m of the depth of water, keep miniflow water；
（b）Antenatal reinforced cultivating：In season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, proceed to cement pit
Reinforced cultivating is carried out, each cement pit throws in 200～300 tail of parent fish, keep 3～5 h/d of pond bath；10 before artificial induced spawning～
15 d guarantee 8 more than h/d of flushing period, to promote parent fish gonad development；Raised using the hand fit of protein content 36%～40%
Material and greenfeed cultivated, feed mixed feed day 2～3 times, day feeding volume for fish body weight 1%～2%, greenfeed is thrown every other day
Feed 1 time；
Further, the cement pit is 40 m of area2～60 m2, 0.5～0.8 m of the depth of water rectangle cement pit, and be equipped with
1.5 one, kw water pumps, keep miniflow water, intake；
（2）Artificial induced spawning：
Selecting the healthy and strong individuality without wound, gonadal maturation from the parent fish of reinforced cultivating carries out artificial induced spawning test：Hasten parturition sharp
Element adopts luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid DOM (DOM)+chorionic-gonadotropin hormone (HCG)
Mix preparation, 2 pin injection systems；Oxytocic drug injected by 1st pin：3 mg LRH-A2+4 mg DOM+100 IU HCG)；2nd pin
Injection oxytocic drug：5 mg LRH-A2+5 mg DOM+1000 IU HCG)；10～12 h of needle gage, milter do not inject the 1st pin,
2nd injection amount for raun 1/2；Under 23 DEG C～27 DEG C water temperature conditions, Effect time is 10～14 h；Female-male proportion be 3～
5:1；
（3）Artificial insemination：Artificial insemination adopts dry method insemination method；
（4）Artificial incubation：
Germ cell after by imbibition is evenly arranged on hatching cloth ovum frame, under 25 DEG C～27 DEG C hatching water temperature conditions, young
Fish is about through 58～60 h membranes；3 age in days prolarvas artificially should not startle；Guarantee to hatch in hatching process water body dissolved oxygen in 5 mg/
More than l；Hatching pattern is using micro- Lotic hatching or the inflation hatching of miniflow water；
（5）Seed early stage is cultivated：
（a）Open-mouthed bait is cultivated：With 2000 m of area2Within, the Tu Chi of 0.6～0.8 m of the depth of water be bait culture pond, starting
What front 8～12 d of artificial induced spawning work carried out bait culture pond levels work；7～9 d after cleaning up the pond, treats that drug toxicity disappears,
New water is injected to 0.5～0.6 m of the depth of water；Every 667 m280～100 kg of fertilizer after fermentation is applied, to cultivate dynamic plant of swimming
Thing；
（b）Larvae cultivation：The zooplankton that 80 mesh sieve in above-mentioned bait culture pond, 10～25 ages in days are fed before 10 age in days of prelarva
The zooplankton with 60 mesh sieved then is fed, and 20 ages in days start the artifical compound feed for taming and dociling food protein content 40%～45%；Young
Cultivation of the fish through 39～51 d, you can obtain specification up to the juvenile fish of 3 more than cm, the juvenile fish is summer flower seed；
（6）First filial generation large size seedling seed is cultivated：
The pool under the first filial generation juvenile fish clear water of 3～5 cm of specification is proceeded to Tu Chi carries out large size seedling seed cultivation and forms；Day feeds
Mixed feed 2～3 times, day feeding volume for fish body weight 3%～5%, heavy property of the mixed feed for protein content 38%～42%
Particulate material；When most of first filial generation seed size is up to 10 cm, seed harvesting sizing screening, the seed of 10 below cm are continued
Tu Chi cultivations are stayed in, the seed of 10 more than cm proceeds to other Tu Chi, carries out standby parent culture；
Further, the native pool area is in 2000 m2Within, 0.8～1.0 m of the depth of water, miniflow water are intake with 60～80 mesh sieves
Silk is filtered；
（7）First filial generation parent forms and cultivation：
（a）First filial generation standby parent form：Feed mixed feed 2～3 times day, day feeding volume for fish body weight 2%～3%, this is matched somebody with somebody
Close heavy property particulate material of the feedstuff for protein content 36%～40%；When most of first filial generation standby parent specification is up to 15 cm, will
Its harvesting carries out second sizing screening, and the standby parent for screening 15 more than cm proceeds to other Tu Chi, carries out first filial generation parent
Cultivate；
Further, parent culture pond is step（6）Described in Tu Chi；
（b）First filial generation parent culture：The same step of breeding method（1）；
（8）The realization of artificial seedling rearing:Using step（1）Operational approach obtains gonad fully-developed first filial generation parent, repeats
Step（2）To step（4）, second filial seed is obtained, artificial seedling rearing is realized.
2. according to claim 1, it is characterised in that step（3）The concrete operation step of described dry method insemination method
For：Raun abdominal part moisture to effector phase is dried, in the container for gently pressing its abdominal part that ovum is clamp-oned dry from gonopore；Use simultaneously
Suction pipe sucks the seminal fluid of extrusion from milter gonopore, is just squeezed with pouring into after 0.7% normal saline dilution of 1 ~ 2 times of volume pH 7.0
In the ovum for going out, then it is gently mixed after 2～3 min of mixing with feather, adds clear water to clean germ cell and go to glue, then incubated
Change.
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| CN107821247A (en) * | 2017-11-29 | 2018-03-23 | 宁波大学 | A kind of whole artificial propagation method of opsariichthys bidenses |
| CN107912340A (en) * | 2017-11-30 | 2018-04-17 | 宁夏润宝禾农业科技有限公司 | A kind of mating system of Yellow River catfish kind |
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| CN114651752A (en) * | 2022-03-13 | 2022-06-24 | 江苏海洋大学 | Method for artificially cultivating Murray cod parent fish |
| CN115349468A (en) * | 2022-09-29 | 2022-11-18 | 福建省淡水水产研究所 | Total artificial propagation method of gobiocyparidae |
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