CN113973608B - Plant cultivation method - Google Patents

Plant cultivation method Download PDF

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Publication number
CN113973608B
CN113973608B CN202111091391.9A CN202111091391A CN113973608B CN 113973608 B CN113973608 B CN 113973608B CN 202111091391 A CN202111091391 A CN 202111091391A CN 113973608 B CN113973608 B CN 113973608B
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light source
plant
equal
seedlings
target
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CN113973608A (en
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刘威
庄喻韬
葛良安
张宪
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Siwei Ecological Technology Hangzhou Co ltd
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Siwei Ecological Technology Hangzhou Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The application provides a plant cultivation method, in particular to the field of plant planting, and the method comprises the following steps: providing a seedling of a target plant; cultivating the seedlings of the target plants by using the corresponding preset culture medium and preset cultivation conditions so as to grow the seedlings of the target plants; the preset cultivation condition comprises that artificial light sources with light source frequency more than or equal to 100KHz and duty ratio less than or equal to 70% are used for irradiating the seedlings of the target plants. By the technical scheme of the method, the electric energy consumption and the related operation energy consumption in the plant cultivation process can be effectively reduced, the light energy utilization rate is improved, and meanwhile, the photosynthesis efficiency of the plant can be effectively improved by regulating and controlling the light source frequency and the duty ratio.

Description

Plant cultivation method
Technical Field
The application relates to the field of new energy planting, in particular to a plant cultivation method.
Background
In the development process of modern agriculture, plant factories are utilized for plant cultivation, and the most promising production mode is provided. The plant factory is a high-efficiency agricultural system for realizing annual continuous production of crops by high-precision environmental control in a facility, and is a labor-saving production mode which utilizes an intelligent computer and an electronic sensing system to automatically control the environmental conditions of plant growth so that the growth and development of plants in the facility are not or rarely restricted by natural conditions. In a plant factory, artificial light is usually used as all or part of light energy sources of a plant cultivation system, a large number of artificial light sources and heat dissipation equipment are required, and the operation energy consumption cost such as light source loss, illumination energy consumption and heat dissipation is high. Therefore, it is desirable to provide an artificial plant cultivation method capable of reducing the corresponding production cost to solve the above existing technical problems.
Disclosure of Invention
In view of the above problems of the prior art, the present application provides a plant cultivation method capable of reducing energy consumption for light source and operation, the method comprising:
providing a seedling of a target plant;
cultivating the seedlings of the target plants by using corresponding preset culture media and preset cultivation conditions so as to enable the seedlings of the target plants to grow; the preset cultivation condition comprises that artificial light sources with the light source frequency more than or equal to 100KHz and the duty ratio less than or equal to 70% are used for irradiating the seedlings of the target plants.
Illustratively, the artificial light source comprises an LED light source.
Illustratively, part or all of the irradiation units of the LED light source are provided with fluorescent powder.
Illustratively, the target plant is a leaf vegetable plant.
Illustratively, the light source frequency is greater than or equal to 500KHz.
Illustratively, the light source frequency is greater than or equal to 1MHz.
Illustratively, the duty cycle is greater than or equal to 10% and less than or equal to 70%.
Illustratively, the duty cycle is greater than or equal to 20% and less than or equal to 60%.
Illustratively, the duty cycle is greater than or equal to 20% and less than or equal to 40%.
Illustratively, the artificial light source has an illumination intensity ranging from 110 to 350 [ mu ] mol · m -2 ·s -1 The photoperiod of the irradiation treatment is 14-16h.
Illustratively, the artificial light source has an illumination intensity in the range of 180-350 [ mu ] mol-m -2 ·s -1
Illustratively, the preset incubation conditions further include: the carbon dioxide concentration in the incubation environment is 500-2000ppm during the photoperiod.
Specifically, the providing of the seedling of the target plant comprises:
sowing seeds in a seedling raising sponge to germinate seeds of the target plants;
and adding seedling culture nutrient solution into the seedling culture sponge for seedling culture to obtain the seedlings of the target plants.
Illustratively, the pH value of the seedling culture nutrient solution is in the range of 5.5-6.5, and the EC range of the seedling culture nutrient solution is in the range of 1.0-1.4ms cm -1
Illustratively, the preset incubation conditions further include: the environment temperature in the cultivation process is 15-30 ℃, and the light period environment temperature in the light period is more than or equal to the dark period environment temperature in the dark period.
Illustratively, the preset incubation conditions further include: the air humidity during the cultivation process is in the range of 60-80%.
Based on the technical scheme, the method has the following beneficial effects:
this application is through providing target plant's seedling, then arrange target plant's seedling in the corresponding culture medium of predetermineeing, and under the condition of cultivating of predetermineeing including utilizing light source frequency more than or equal to 100KHz and duty cycle less than or equal to 70% artificial light source to shine the processing, cultivate target plant's seedling, so that target plant's seedling is grown, can effectively reduce the power consumption and the relevant operation energy consumption of plant cultivation in-process, improve the light energy utilization ratio, can effectively improve the photosynthesis efficiency of plant through regulation and control light source frequency and duty cycle simultaneously.
Drawings
In order to more clearly illustrate the technical solution of the present application, the drawings used in the description of the embodiment or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the application, and that for a person skilled in the art, other drawings can also be derived from them without inventive effort.
FIG. 1: the embodiment of the application provides a flow diagram of a plant cultivation method;
FIG. 2: the lettuce planting example image provided by the embodiment of the application;
FIG. 3: the method comprises the steps of obtaining a histogram of fresh weight results of overground parts of leaf menu plants of the leaf mallotus philippinensis under the condition of a table;
FIG. 4: a histogram of vitamin C content results in lettuce plants obtained under the conditions provided in one embodiment of the present application;
FIG. 5 is a schematic view of: a histogram of the light energy utilization efficiency results obtained under the conditions of the table provided by one embodiment of the present application;
FIG. 6: according to the method, a histogram of fresh weight results of overground parts of the leaf menu plants is obtained under the surface two conditions;
FIG. 7 is a schematic view of: a histogram of vitamin C content results in leaf lettuce plants obtained under the table two conditions provided by one embodiment of the present application;
FIG. 8: a histogram of the light energy utilization efficiency results obtained under the two conditions of the table provided by one embodiment of the present application;
FIG. 9: the fresh weight result histogram of the overground part of the leaf menu plant obtained under the condition of the table three provided by one embodiment of the application is shown in the drawing;
FIG. 10: a histogram of vitamin C content results in lettuce plants obtained under the table three conditions provided by one embodiment of the present application;
FIG. 11: the present application provides a histogram of the light energy utilization efficiency results obtained under the table three conditions.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
For the following defined terms, these definitions shall be applied, unless a different definition is given in the claims or elsewhere in this application. All numerical values, whether explicitly indicated or not, are herein defined as modified by the term "about". The term "about" generally refers to a range of values that one of ordinary skill in the art would consider equivalent to the recited value to produce substantially the same property, function, result, etc. A numerical range indicated by a low value and a high value is defined to include all numbers subsumed within the numerical range and all subranges subsumed within the numerical range.
The plant cultivation method of the present application is described below with reference to fig. 1, which comprises:
s101: seedlings of the target plant are provided.
In the embodiment of the present application, the target plant may be a plant that can be cultivated in a semi-artificial or total-artificial environment such as a plant factory. In some embodiments, the target plants include, but are not limited to, leaf vegetables, fruits, and flower plants, among others. In one embodiment, the target plant is a leaf vegetable, such as leaf lettuce, chinese cabbage, spinach, rape, celery or wheatgrass.
In some embodiments, step S101 may specifically include:
s1011: sowing is carried out in the seedling raising sponge to germinate the seeds of the target plants.
In practical application, seeds of target plants can be sown in the seed-growing holes of the water-soaked seedling-growing sponge for germination. In some cases, the seeds of the target plants may be subjected to germination acceleration treatment, such as irradiation treatment using an artificial light source. It should be noted that the artificial light source, the light source parameters, the irradiation conditions, and other growth environment conditions used in the germination accelerating process may be the same as or different from those in the seedling cultivation process described below, and the present application is not limited thereto.
In one embodiment, the seeding process specifically comprises: soaking seedling raising sponge in water, sowing seeds of a target plant in each seedling raising hole, covering a transparent protective cover or a protective film on a seedling raising plate, and placing the seedling raising plate on a seedling raising frame.
S1012: and adding seedling culture nutrient solution into the seedling culture sponge to carry out seedling culture to obtain the seedlings of the target plants.
In practical application, after the seeds germinate, the seedlings are raised through the seedling raising nutrient solution. Specifically, nutrient solution supplementation can be performed based on a preset time interval in the seedling raising process, for example, the preset time interval can be 2-3 days. Specifically, after the seeds germinate, part or all of the moisture in the seedling raising sponge can be controlled to be dry, for example, the moisture on the surface layer of the seedling raising sponge can be controlled to be dry, and the seedling raising nutrient solution is added into the seedling raising tray. It can be understood that the preset time interval, the components and concentration of the seedling raising nutrient solution, etc. can be set according to the actual requirements of the target plants, such as the types of the target plants, etc., and the application is not limited herein.
In some embodiments, the seedling nutrient solution may include sodium chloride, potassium hydroxide, and the like. In some embodiments, the pH of the nutrient solution for growing seedlings is in the range of 5.5 to 6.5, and the EC of the nutrient solution for growing seedlings is in the range of 1.0 to 1.4ms cm -1 . It should be noted that the above numerical ranges are only examples, and the PH and EC ranges of the seedling nutrient solution can be set to other numerical ranges according to actual requirements, and the present application is not limited herein.
S102: and cultivating the seedlings of the target plants by using the corresponding preset culture medium and the preset cultivation conditions so as to grow the seedlings of the target plants. The preset cultivation condition comprises that artificial light sources with light source frequency more than or equal to 100KHz and duty ratio less than or equal to 70% are used for irradiating seedlings of the target plants. Compared with the cultivation condition of irradiation treatment by using a direct current panel light source (LED continuous light), under the irradiation condition, the light utilization efficiency is improved by at least 10%, the fresh weight of the target plant is increased by at least 15% within the same growth period, and the content of the secondary metabolite of the target plant is increased by at least 21% within the same growth period.
In some embodiments, the source frequency may be in the frequency range or in frequency point values of 100KHz or greater, such as 100KHz-20MHz,200KHz-20MHz,500KHz-20MHz,1MHz-20MHz,2MHz, 3MHz-20MHz,5MHz-20MHz,100KHz-15MHz,500KHz-15MHz, etc., or 100KHz,200KHz-,500KHz,1MHz,2MHz,3MHz,4MHz,5MHz,6MHz,8MHz,10MHz,15MHz, etc., which are not enumerated herein.
In some embodiments, the duty cycle may be any value greater than 0 and equal to or less than 70%, which is not enumerated herein.
In practical application, by mixingThe duty ratio of the artificial light source is regulated to be below 70%, so that the power consumption and the heat productivity of the light source can be effectively reduced, and the electric energy consumption and the heat dissipation energy consumption are further reduced. In addition, almost all dry matter in plants is accumulated by assimilation of carbon fixation in photosynthesis, which is a mechanism of energy and substance conversion, in which light energy is first converted into electric energy, unstable chemical energy in the form of ATP, NADPH, and the like is generated through electron transfer, and finally converted into stable chemical energy and stored in a sugar compound. The process of photosynthesis is divided into light reaction (light reaction) which requires light irradiation, involves photolysis of water, photosynthetic phosphorylation, and the like, and dark reaction (dark reaction) which does not require light irradiation, involves CO 2 Fixing and the like. By regulating the duty ratio and the frequency of the artificial light source to the preset range, the photoelectric conversion efficiency in the light reaction process and the metabolite accumulation in the dark reaction process are favorably improved, and the photosynthesis efficiency is further improved.
In the embodiment of the application, the components and the forms of the preset culture medium can be selected according to the requirements of the target plants. Specifically, the predetermined medium may be a solid medium, a liquid medium, a semi-solid medium, or the like. In some embodiments, a liquid medium having a composition similar to or different from that of the above-described nutrient solution for raising seedlings may be prepared as the predetermined medium based on actual needs. By controlling the cultivation environmental conditions to the preset cultivation conditions, the seedlings grow to the target form or the target growth state under the preset cultivation conditions, so that the cultivation and the harvesting of the target plants are realized.
In the embodiment of the application, after the seedling of the target plant is obtained through the steps of sowing, seedling raising and the like, the seedling of the target plant can be transplanted. In one embodiment, the seedling raising sponge can be divided into independent sponge blocks by taking a single seedling plant as a unit, a plurality of single seedlings carrying the sponge blocks are obtained, then the single seedlings are planted in the planting holes of the planting plate, and the root systems of the seedlings extend into a preset culture medium, so that the seedlings are transplanted.
In some embodiments, the quality of the artificial light source can be selected according to the requirements of the target plant, such as the type of target plant, growth stage and product requirements, for example, the artificial light source used can emit one or a combination of red light, blue light, white light, yellow light, green light and far-infrared light.
In some embodiments, the duty cycle of the artificial light source may be controlled by chopping, for example by PWM modulation.
In some embodiments, the artificial light source comprises an LED light source. Compared with the traditional artificial light source such as a fluorescent lamp, a halide lamp, a sodium lamp and the like, the LED light source is long in service life, stable in illumination intensity and small in heating, the service life of the light source can be further prolonged, the heating amount can be further reduced by controlling the frequency and the duty ratio of the light source, the damage of the heat dissipation of the light source to plants is avoided, and meanwhile, the energy consumption of operation such as heat dissipation can be reduced.
In addition, for a normal light source without a diode, after power is cut off, current can oscillate in a circuit, and thus, the time from light source to dark is longer. And the LED light source does not have the problem that above-mentioned circuit vibrates, and the light source darkens more rapidly, is favorable to setting up (more than or equal to 100 KHz) through the high frequency and realizes the stroboscopic of light, and then improves photosynthesis efficiency. In addition, the LED light source emits light to generate a fluorescence phenomenon and has light persistence, so that the light cannot be extinguished immediately after the current suddenly drops, a light gradual change process exists, compared with a low frequency, the waveform change of a chopped wave light signal under a high frequency is more gentle, and the photosynthesis of plants is more facilitated.
And, when light takes place the light and shade change, be favorable to the photoelectric conversion of plant, consequently the duty cycle is lower, is favorable to the formation and the accumulation of metabolite more, through combining together high frequency setting and duty cycle regulation and control, can effectively improve photosynthesis efficiency, light energy utilization and metabolite accumulation when reducing the energy consumption, and then improve plant output and metabolite content, realize the high-efficient growth and the nutritive value of plant and promote.
In some embodiments, some or all of the illumination units of the LED light sources are provided with phosphors therein. Through reasonable setting of the type, content and other parameters of the fluorescent powder, the waveform of the chopped light signal can be adjusted, and then a better waveform beneficial to the growth of the seedlings of the target plants is determined through experiments, so that the light energy utilization rate and the photosynthesis efficiency are further improved.
Based on some or all of the above embodiments, in some embodiments, the frequency of the light source is greater than or equal to 500KHz. Compared with the cultivation condition that the direct current panel light source (50 Hz) is used for irradiation treatment, under the irradiation condition that the light source frequency is larger than or equal to 500KHz and the duty ratio is smaller than or equal to 70%, the light utilization efficiency is at least improved by 14%, the fresh weight of the target plant is at least increased by 17% within the same growth time period, and the content of the secondary metabolite of the target plant is at least increased by 26% within the same growth time period.
In other embodiments, the source frequency is greater than or equal to 1MHz. Compared with the cultivation condition of irradiation treatment by using a direct current panel light source, under the irradiation conditions that the frequency of the light source is more than or equal to 1MHz and the duty ratio is less than or equal to 70%, the light utilization efficiency is at least improved by 16%, the fresh weight of the target plant is at least increased by 23% in the same growth period, and the content of secondary metabolites of the target plant is at least increased by 33% in the same growth period.
In other embodiments, the source frequency is greater than or equal to 10MHz. Compared with the cultivation condition of irradiation treatment by using a direct current panel light source, under the irradiation conditions that the frequency of the light source is more than or equal to 10MHz and the duty ratio is less than or equal to 70%, the light utilization efficiency is at least improved by 24%, the fresh weight of the target plant is at least increased by 28% in the same growth period, and the content of secondary metabolites of the target plant is at least increased by 50% in the same growth period.
In one embodiment, when the target leaf vegetable plant is leaf vegetable, the light source frequencies of the LED light sources are adjusted to 100khz,500khz,1mhz,5mhz,7mhz,10mhz and 15MHz, and the duty ratio is 60% by chopper modulation, the fresh weight of the leaf vegetable obtained in the same growth is respectively increased by 34.43%,35.48%,41.13%,45.16%,48.39%,50.03% and 55.65% compared with that of the direct current panel light source, the vitamin C content of the leaf vegetable in the same growth is respectively increased by 24.07%,32.98%,42.15%,54.5%,64.8%,72.15% and 81.41%, and the light utilization efficiency is respectively increased by 17.15%,18.64%,25.87%,33.9%,39.68%,44.46% and 49.64%. Therefore, under the condition that the light source conditions such as duty ratio and the like and other growth conditions are not changed, the fresh weight, the vitamin C content and the light utilization efficiency of the leaf lettuce are obviously improved along with the increase of the light source frequency.
Based on some or all of the above embodiments, in some embodiments, the duty cycle is greater than or equal to 10% and less than or equal to 70%. Under the condition of duty ratio, when the light source frequency is more than or equal to 100KHz, compared with the cultivation condition of irradiation treatment by a direct-current panel light source, the light utilization efficiency is at least improved by 10%, the fresh weight of the target plant is at least increased by 15% in the same growth time period, and the content of secondary metabolites of the target plant is at least increased by 21% in the same growth time period. In some embodiments, the duty cycle may be any value greater than 10% and less than or equal to 70%, which is not enumerated herein.
In other embodiments, the duty cycle is greater than or equal to 20% and less than or equal to 60%. Under the condition of duty ratio, when the frequency of the light source is more than or equal to 100KHz, compared with the cultivation condition of irradiation treatment by a direct-current panel light source, the light utilization efficiency is improved by at least 15%, the fresh weight of the target plant is increased by at least 17% within the same growth time period, and the secondary metabolite content of the target plant is increased by at least 29% within the same growth time period. Similarly, the duty cycle may be any of more than 20% and 60% or less.
In other embodiments, the duty cycle is greater than or equal to 20% and less than or equal to 40%. Under the condition of duty ratio, when the frequency of the light source is more than or equal to 100KHz, compared with the cultivation condition of irradiation treatment by a direct-current panel light source, the light utilization efficiency is at least improved by 22%, the fresh weight of the target plant is at least increased by 28% within the same growth time period, and the secondary metabolite content of the target plant is at least increased by 36% within the same growth time period. Similarly, the duty cycle may be any value greater than 20% and equal to or less than 40%.
In some embodiments, the duty cycle may also be greater than or equal to 25% and less than or equal to 55%, greater than or equal to 25% and less than or equal to 50%, greater than or equal to 25% and less than or equal to 45%, greater than or equal to 25% and less than or equal to 40%, or greater than or equal to 25% and less than or equal to 35%, etc.
In practical application, under the condition that light source conditions such as light source frequency and the like and other growth conditions are not changed, the fresh weight, the secondary metabolite content and the light utilization efficiency of a target plant are obviously improved along with the reduction of the duty ratio. In one embodiment, when the target plant is leaf lettuce, the frequency of the LED light source is modulated to 10MHz by chopping, and the duty ratios are 70%,60%,40%, and 20%, respectively, the fresh weight of the leaf lettuce obtained in the same growth period is increased by 37.5%, 47.36%, 57.48%, and 67.4%, respectively, compared with the condition of the dc panel light source, the vitamin C content of the leaf lettuce in the same growth period is increased by 41.10%, 87.89%, 102.5%, and 120.5%, and the light utilization efficiency is increased by 25.89%, 46.15%, 52.23%, and 62.23%, respectively, and the difference reaches a significant level.
Based on some or all of the above embodiments, in some cases, it is also necessary to control the illumination intensity range and the light period of the artificial light source to achieve the optimal growth conditions of the target plant seedling. In some embodiments, the artificial light source has an illumination intensity in the range of 110-350 μmol-m -2 ·s -1 The photoperiod of the irradiation treatment is 14 to 16 hours, for example, 14h,14.5h,15h,15.5h or 16h, and the plants may be subjected to dark treatment outside the photoperiod, and the dark period may be 8 to 10 hours.
In other embodiments, the artificial light source may have an illumination intensity in the range of 180-350 μmol-m -2 ·s -1
In other embodiments, the artificial light source may have an illumination intensity in the range of 200-350 μmol-m -2 ·s -1
In other embodiments, the artificial light source may have an illumination intensity in the range of 180-300. Mu. Mol. M -2 ·s -1
In other embodiments, the artificial light source may have an illumination intensity in the range of 180-250 μmol. M -2 ·s -1
In some embodiments, the illumination intensity may also range from 130 to 350. Mu. Mol. M -2 ·s -1 ,150-350μmol·m -2 ·s -1 ,220-350μmol·m -2 ·s -1 ,250-350μmol·m -2 ·s -1 ,130-300μmol·m -2 ·s -1 Or 150 to 300. Mu. Mol. M -2 ·s -1 And the like.
Specifically, when the target plant is a leaf vegetable, the light source frequency may be set to 100KHz or more, the duty ratio may be set to 10% to 60%, and the light intensity may be set to 200-300. Mu. Mol. M -2 ·s -1
It is understood that too low or too high an intensity of light is not beneficial to the growth of the plant. Too low light intensity is detrimental to plant biomass and yield development. Under the condition of too low light intensity, the transportation efficiency and the transportation amount of the carbohydrate in the leaves are reduced, and simultaneously, under the self-regulation mechanism of weak light stress, the distribution of the photosynthetic products in the plants is also changed correspondingly, such as more biomass of the plants is distributed to the overground part, the plant height is increased, the stem thickness is thinned, the leaf length and the leaf width are elongated, and the leaf area is expanded, so that more light energy can be obtained upwards and around. When the illumination intensity is higher than a certain value, leaves of plants are easy to burn and burn, and the photosynthesis efficiency and the utilization rate are also reduced. If the light intensity is higher than or equal to 220 mu mol.m under the condition of a direct current panel light source -2 ·s -1 When the utility model is used, the water is discharged, leaf of Chinese lettuce produces burnt edge.
Adopt illumination conditions such as light source frequency and duty cycle that this application set up, not only can effectively improve plant output, secondary metabolite accumulative total speed and content, photosynthesis efficiency and light energy utilization rate etc. can also improve target plant to illumination intensity's accommodation and to the tolerance of high light intensity by a wide margin, make the leaf dish plant grow under higher light intensity, when improving growth rate, avoid the plant to burn. In some cases, the intensity of light is as high as 350. Mu. Mol. M -2 ·s -1 In time, the plant can still grow normally without burning phenomena such as the burnt edge of the leaf and the like.
Based on some or all of the above embodiments, in some embodiments, the yield of the target plant can be further increased by supplementing carbon dioxide into the air, and accordingly, the preset cultivation conditions further include: the carbon dioxide concentration in the incubation environment is 500-2000ppm during the photoperiod. In some embodiments, during the dark cycle, carbon dioxide replenishment is not performed.
In some embodiments, the carbon dioxide concentration in the incubation environment is 800-1500ppm. By reasonably setting CO 2 The concentration and the light condition parameters are matched, so that the adaptability and the utilization rate of the plant to light can be further improved.
Based on some or all of the above embodiments, in some embodiments, the preset incubation conditions further include: the environmental temperature in the cultivation process is 15-30 ℃, and the light-period environmental temperature in the photoperiod is more than or equal to the dark-period environmental temperature in the dark period, so that the temperature change of the natural environment is more approximate, and the energy consumption is favorably reduced.
In some embodiments, the ambient temperature during incubation is 15-25 ℃. In one embodiment, the light ambient temperature is 22 ± 2 ℃ and the dark ambient temperature is 18 ± 2 ℃.
Based on some or all of the above embodiments, in some embodiments, the preset incubation conditions further include: the air humidity during the cultivation process is in the range of 60-80%.
It should be noted that, when the parameter of the cultivation condition or the cultivation environment of the present application relates to a numerical range, such as a frequency range, a duty ratio range, a time, a temperature range, or a humidity range, it indicates that the parameter may be any point value within the corresponding numerical range, and may be set according to the type and the actual requirement of the target plant, which is not enumerated herein.
In conclusion, this application is through providing the seedling of target plant, then arrange the seedling of target plant in the corresponding culture medium of predetermineeing, and under the condition of cultivating including utilizing light source frequency more than or equal to 100KHz and duty cycle less than or equal to 70% artificial light source to shine the cultivation of predetermineeing of handling, cultivate the seedling of target plant, so that the seedling growth of target plant, can effectively reduce the power consumption and the relevant operation energy consumption of plant cultivation in-process, improve the light utilization efficiency, can effectively improve the photosynthesis efficiency of plant through regulation and control light source frequency and duty cycle simultaneously.
The plant cultivation method of the present application will be described below with reference to fig. 2 to 11, taking the target plant as leaf vegetable leaf lettuce as an example, and fig. 2 shows a diagram of leaf vegetable leaf lettuce plants cultivated by the cultivation method of the present application.
Example 1
The embodiment provides a plant cultivation method, which specifically comprises the following steps:
s1: and (4) sowing, namely sowing in the seedling raising sponge to make the seeds of the leaf lettuce germinate. Wherein the seedling raising sponge is water-soaked sponge, a plurality of seed raising holes are formed in the seedling raising sponge, and one leaf lettuce seed is sowed in each seed raising hole.
Specifically, the seedling raising sponge can be placed in the seedling raising tray, and the seedling raising tray is covered with a transparent protective cover or a protective film.
In some cases, an artificial light source is used for irradiation treatment to accelerate germination of leaf lettuce seeds in the seedling raising sponge.
S2: after the seeds germinate, controlling part or all of water in the seedling raising sponge to be dry, and adding a seedling raising nutrient solution into the seedling raising sponge to raise seedlings to obtain seedlings of the leaf lettuce. The seedling nutrient solution can include, but is not limited to, sodium chloride and potassium hydroxide. And in the seedling raising process, nutrient solution replenishing is carried out every 2-3 days.
Specifically, the pH value range of the seedling culture nutrient solution is 5.5-6.5, the EC range is 1.0-1.4ms cm -1
S3: providing a preset culture condition and a preset culture medium corresponding to the seedlings of the leaf lettuce, and transplanting the seedlings of the leaf lettuce into the preset culture medium to culture the seedlings of the leaf lettuce to obtain leaf lettuce plants. Referring to fig. 2, fig. 2 shows the lettuce plants obtained in this embodiment.
Wherein the preset cultivation conditions comprise:
(1) Irradiating the seedlings of leaf lettuce with artificial light source with frequency of 100KHz or more, duty ratio of 70% or less, and illumination intensity of 110-350 μmol/m -2 ·s -1 The photoperiod is 14-16h.
(2) Carbon dioxide is supplemented in the photoperiod, and the concentration of carbon dioxide in the cultivation environment is 800-1500ppm.
(3) The ambient temperature during incubation was 15-25 ℃. In some cases, the light ambient temperature is 22 ± 2 ℃ and the dark ambient temperature is 18 ± 2 ℃.
(4) The air humidity range of the cultivation environment is 60-80%.
In this embodiment, an LED light source is provided as an artificial light source, lighting conditions in which light source frequencies are set to 100khz,500khz,1mhz,5mhz,7mhz,10mhz, and 15MHz, respectively, and duty ratios are 60% are set by chopping, seedling cultivation of leaf lettuce is performed under conditions in which other test conditions are almost the same, and leaf lettuce plants corresponding to each condition are obtained, respectively.
It should be noted that, this embodiment also provides a dc panel LED light source (50 Hz, continuous light) without performing chopping processing as a dc condition control group, and an illumination condition with a light source frequency of 1 KHz/duty ratio of 60% set by chopping as a low frequency control group, and the specific test conditions refer to table one below.
Watch 1
Figure BDA0003267622700000111
Referring to fig. 3, fig. 3 shows the fresh weight results of the overground parts of the leaf menu plants of rye obtained under the conditions of the table. As can be seen from the results in fig. 3, when the duty ratio is 60% and the chopping frequencies are 100khz,500khz,1mhz,5mhz,7mhz,10mhz and 15MHz, respectively, the overground fresh weight of the single leaf of leaf lettuce is increased by 34.43%,35.48%,41.13%,45.16%,48.39%,50.03% and 55.65%, respectively, compared with the dc control group, the differences are significant. Along with the increase of chopping frequency, the fresh weight of the leaf lettuce is correspondingly increased, the fresh weight of the leaf lettuce under the condition of 15MHz is better, and the plant yield is effectively improved.
Referring to fig. 4, fig. 4 shows the results of vitamin C content in lettuce plants obtained under the conditions of the table. Compared with a direct current control group, when the duty ratio is 60%, and the chopping frequencies are 100KHz,500KHz,1MHz,5MHz,7MHz,10MHz and 15MHz, the content of the leaf lettuce vitamin C is respectively increased by 24.07%,32.98%,42.15%,54.5%,64.8%,72.15% and 81.41%, and the difference reaches a significant level. Along with the increase of chopping frequency, the content of vitamin C is correspondingly increased, and the content of vitamin C in leaf lettuce under the condition of 15MHz is better.
Referring to fig. 5, fig. 5 shows the result of the light energy utilization efficiency obtained under the condition shown in the table. Compared with the direct current control group, when the duty ratio is 60%, and the chopping frequencies are 100KHz,500KHz,1MHz,5MHz,7MHz,10MHz and 15MHz, the light energy utilization efficiency is respectively improved by 17.15%,18.64%,25.87%,33.9%,39.68%,44.46% and 49.64%, and the difference is obvious. The light utilization efficiency is increased along with the increase of the chopping frequency, and the light energy utilization efficiency under the condition of 15M is higher.
In conclusion, by setting the high frequency and the proper duty ratio, the energy consumption can be reduced, the photosynthesis efficiency and the energy utilization rate can be improved, and the plant yield and the accumulation speed and the content of secondary metabolites such as vitamin C can be effectively improved.
Example 2
The present embodiment provides a plant cultivation method, the specific steps and partial cultivation conditions of the cultivation method are similar to those of the above embodiment 1, and are not repeated herein, and the differences between the present embodiment and embodiment 1 are described below, specifically as follows:
in this embodiment, an LED light source is provided as an artificial light source, the light source frequency is set to 10MHz by chopping, the duty ratios are set to 70%,60%,40% and 20% respectively, and under the conditions that other test conditions are almost the same, seedling cultivation of leaf lettuce is performed, so as to obtain leaf lettuce plants corresponding to each condition respectively.
It should be noted that, this embodiment also provides a dc panel LED light source (50 Hz, continuous light) without performing chopping processing as a dc condition control group, and the following table two is referred to for specific test conditions.
Watch 2
Figure BDA0003267622700000121
Referring to FIG. 6, FIG. 6 shows the fresh weight results of the aerial parts of the leaf of Menu mallotus obtained under the conditions of Table two. As can be seen from the results in fig. 6, when the light source frequency is 10MHz and the duty ratios are 70%,60%,40% and 20% respectively, the fresh weight of the leaf lettuce single plant on the ground is increased by 37.5%, 47.36%, 57.48% and 67.4% respectively, and the difference is significant compared with the dc control group. Along with the reduction of the duty ratio, the fresh weight of the leaf lettuce is correspondingly increased, the fresh weight of the leaf lettuce is better under the condition of 20 percent, and the plant yield is effectively improved.
Referring to fig. 7, fig. 7 shows the results of vitamin C content in lettuce plants obtained under the two conditions of table. Compared with a direct current control group, when the light source frequency is 10MHz, the duty ratios are respectively 70%,60%,40% and 20%, the vitamin C content of the leaf lettuce is respectively improved by 41.10%, 87.89%, 102.5% and 120.5%, and the difference reaches a significant level. Along with the reduction of the duty ratio, the content of the vitamin C is correspondingly increased, and the content of the vitamin C in the leaf lettuce is better under the condition of 20 percent.
Referring to fig. 8, fig. 8 shows the results of the light energy utilization efficiency obtained under the two conditions of the table. Compared with a direct current control group, when the light source frequency is 10MHz and the duty ratios are 70%,60%,40% and 20%, the light energy utilization efficiency is respectively improved by 25.89%, 46.15%, 52.23% and 62.23%, and the difference is obvious. The light utilization efficiency is increased gradually along with the reduction of the duty ratio, and the light energy utilization efficiency under the condition of 20 percent is higher.
In conclusion, by setting high frequency and adjusting duty ratio, the photosynthesis efficiency and the energy utilization rate can be improved while the energy consumption is reduced, and the plant yield and the accumulation speed and the content of secondary metabolites such as vitamin C and the like can be effectively improved.
Example 3
The present embodiment provides a plant cultivation method, the specific steps and partial cultivation conditions of the cultivation method are similar to those of the above embodiment 1, and are not repeated herein, and the differences between the present embodiment and embodiment 1 are described below, specifically as follows:
in this embodiment, an LED light source is provided as an artificial light source, the light source frequencies are set to 100khz,500khz,1mhz,5mhz,7mhz,10mhz and 15MHz by chopping, and the lighting conditions with the duty ratio of 40% are set, and the seedling cultivation of leaf lettuce is performed under the conditions that other test conditions are almost the same, so as to obtain leaf lettuce plants corresponding to each condition. And an LED light source is provided as an artificial light source, a direct current panel LED light source (50 Hz, continuous light) without chopping processing is used as a direct current condition comparison group, and the specific test conditions refer to the following table III.
Watch III
Figure BDA0003267622700000131
Referring to fig. 9, fig. 9 shows the fresh weight results of the overground parts of the leaf menu plants of rye obtained under the conditions of the table. As can be seen from the results in fig. 9, when the duty ratio was 60% and the chopping frequencies were 100khz,500khz,1mhz,5mhz,7mhz,10mhz and 15MHz, respectively, the above-ground fresh weights of the single leaf lettuce plants were increased by 35.43%,42.48%,45.13%,50.16%,58.03%,60.4% and 61.65%, respectively, as compared to the dc control group, and the differences were all significant. Along with the increase of chopping frequency, the fresh weight of the leaf lettuce is correspondingly increased, the fresh weight of the leaf lettuce under the condition of 15MHz is better, and the plant yield is effectively improved.
Referring to fig. 10, fig. 10 shows the results of vitamin C content in lettuce plants obtained under the conditions of table i. Compared with a direct current control group, when the duty ratio is 60%, and the chopping frequencies are 100KHz,500KHz,1MHz,5MHz,7MHz,10MHz and 15MHz, the content of the lettuce vitamin C is respectively increased by 40.86%,51.3%,68.69%,77.39%,82.43%,86.6% and 95.52%, and the difference reaches a significant level. Along with the increase of chopping frequency, the content of vitamin C is correspondingly increased, and the content of vitamin C in the leaf lettuce under the condition of 15MHz is better.
Referring to fig. 11, fig. 11 shows the results of the light energy utilization efficiency obtained under the conditions shown in the table. Compared with the direct current control group, when the duty ratio is 60%, and the chopping frequencies are 100KHz,500KHz,1MHz,5MHz,7MHz,10MHz and 15MHz, the light energy utilization efficiency is respectively improved by 30.43%,34.3%,39.17%,40.57%,45.68%,53.62% and 60.64%, and the difference is obvious. The light utilization efficiency is increased along with the increase of the chopping frequency, and the light energy utilization efficiency under the condition of 15M is higher.
In conclusion, by setting the conditions of high frequency and proper duty ratio, the photosynthesis efficiency and the energy utilization rate can be improved while the energy consumption is reduced, and the plant yield and the accumulation speed and content of secondary metabolites such as vitamin C and the like are effectively improved.
Example 4
The present embodiment provides a plant cultivation method, the specific steps and partial cultivation conditions of the cultivation method are similar to those of the above embodiment 1, and are not repeated herein, and the differences between the present embodiment and embodiment 1 are described below, specifically as follows:
in this embodiment, an LED light source is provided as an artificial light source, the frequency of the light source is set to 100KHz or more by chopping, the duty ratio is 60%, and the light period is controlled to 16h with the light intensities of 180,200,250,300,350, and 400 μmol · m, respectively -2 ·s -1 And culturing seedlings of the leaf lettuce under the conditions of almost the same other test conditions to respectively obtain leaf lettuce plants corresponding to each condition.
It should be noted that this example also provides a 16h photoperiod without chopping, and light intensities of 180,200,250,300,350 and 400. Mu. Mol. M -2 ·s -1 The DC panel LED light source (50 Hz, continuous light) is used as a DC condition control group, the frequency of the light source obtained by chopping is less than or equal to 1KHz, the duty ratio is 60 percent, the light period is 16h, and the illumination intensity is respectively 180,200,250,300,350 and 400 mu mol.m -2 ·s -1 As a low frequency control group.
Please refer to table four below, which shows the burn condition of the lettuce plants and the corresponding burnt edge rating results under the above-mentioned respective sets of conditions. From the results in Table IV, it is understood that the light intensity is 220. Mu. Mol. M or more under the DC condition -2 ·s -1 <xnotran>, 16h , , ; </xnotran> By high-frequency chopping (chopping frequency of not less than 100KHz, duty ratio of 60% and illumination duration of 16 h), illumination conditions of light and shade alternation are provided for seedlings, and high light and shade alternation can be effectively reducedLight intensity on leaf of Chinese lettuce the burning condition of the leaves is that the leaves are burnt, the tolerance of the plant to the illumination intensity is improved; while low-frequency chopping (chopping frequency less than or equal to 1K, duty ratio of 60 percent and illumination time length of 16 h) has weak effect on relieving the leaf edge scorching symptom under the high light intensity condition, and when the illumination intensity is more than or equal to 250 mu mol.m -2 ·s -1 <xnotran>, , , . </xnotran>
Watch four
Figure BDA0003267622700000151
In addition, with the aforementioned embodiments 1 to 4, it can be known that by setting appropriate illumination intensity, light source frequency and duty ratio, under the mutual cooperation of various conditions, the photosynthesis efficiency, the energy utilization rate, the plant yield and the secondary metabolite content can be effectively improved while the light intensity adaptability of the plant is enhanced, and the efficient high-quality and efficient growth of the plant can be realized.
The foregoing description has disclosed fully embodiments of the present application. It should be noted that those skilled in the art will be able to make modifications to the embodiments of the present application without departing from the scope of the appended claims. Accordingly, the scope of the claims of the present application is not to be limited to the particular embodiments described above.

Claims (13)

1. A method of growing a plant, the method comprising:
providing a seedling of a target plant;
cultivating the seedlings of the target plants by using corresponding preset culture media and preset cultivation conditions so as to enable the seedlings of the target plants to grow; the preset cultivation condition comprises that artificial light sources with light source frequency more than or equal to 100KHz and duty ratio more than or equal to 20% and less than or equal to 40% are used for irradiating the seedlings of the target plants.
2. The method of claim 1, wherein the artificial light source comprises an LED light source.
3. The method according to claim 2, wherein some or all of the illumination units of the LED light sources are provided with phosphors.
4. The method of claim 1, wherein the target plant is a leaf vegetable plant.
5. The method of any of claims 1-4, wherein the light source frequency is greater than or equal to 500KHz.
6. The method of any of claims 1-4, wherein the light source frequency is 1MHz or greater.
7. The method of claim 1, wherein the artificial light source has an illumination intensity in the range of 110-350 μmol -2 •s -1 The photoperiod of the irradiation treatment is 14-16h.
8. The method of any one of claims 1-4, wherein the artificial light source has an illumination intensity in the range of 180-350 μmol -2 •s -1
9. The method of claim 7, wherein the preset incubation conditions further comprise: the carbon dioxide concentration in the incubation environment is 500-2000ppm during the photoperiod.
10. The method of claim 1, wherein the providing a seedling of the target plant comprises:
sowing in a seedling raising sponge to germinate seeds of the target plants;
and adding a seedling culture nutrient solution into the seedling culture sponge for seedling culture to obtain seedlings of the target plants.
11. The method as claimed in claim 10, wherein the pH value of the nutrient solution for raising seedlings is in the range of 5.5-6.5, and the EC range of the nutrient solution for raising seedlings is in the range of 1.0-1.4ms -1
12. The method of claim 7, wherein the preset incubation conditions further comprise: the environment temperature in the cultivation process is 15-30 ℃, and the light period environment temperature in the light period is more than or equal to the dark period environment temperature in the dark period.
13. The method of claim 1, wherein the preset incubation conditions further comprise: the air humidity during the cultivation process is in the range of 60-80%.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538257A (en) * 2016-11-18 2017-03-29 西北农林科技大学 A kind of plant cultivation method and system
CN108575711A (en) * 2018-03-30 2018-09-28 华南农业大学 A kind of method of artificial light source plant factor water planting romaine lettuce
CN110495318A (en) * 2019-09-25 2019-11-26 福建省中科生物股份有限公司 A kind of illumination method of regulation plants ' reproduction development
CN110521566A (en) * 2019-09-25 2019-12-03 福建省中科生物股份有限公司 A kind of luminous environment regulation method of regulation plant metabolism substance

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538257A (en) * 2016-11-18 2017-03-29 西北农林科技大学 A kind of plant cultivation method and system
CN108575711A (en) * 2018-03-30 2018-09-28 华南农业大学 A kind of method of artificial light source plant factor water planting romaine lettuce
CN110495318A (en) * 2019-09-25 2019-11-26 福建省中科生物股份有限公司 A kind of illumination method of regulation plants ' reproduction development
CN110521566A (en) * 2019-09-25 2019-12-03 福建省中科生物股份有限公司 A kind of luminous environment regulation method of regulation plant metabolism substance

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