CN113820484A - 一种家用快速检测皮肤癣菌的测试条制备方法 - Google Patents
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Abstract
本发明公开了一种家用快速检测皮肤癣菌的测试条制备方法,主要技术要点包含:获得皮肤癣菌抗原;制备皮肤癣菌单抗;制备抗原抗体反应的胶体金试剂条;制备临床样品处理液;配备完整的试剂条套装及使用说明书。本发明采用胶体金试剂条的方式来快速检测皮肤癣菌,适合于家用或基层单位使用,自检是否患了脚气或灰指甲等皮肤癣菌感染,然后再互联网医院问诊或自行去药店或网上购买抗真菌药物,可有效减少浅部真菌感染误诊误治的发生以及去医院就诊的经济和时间成本。
Description
技术领域
本发明涉及一种家用快速检测皮肤癣菌的测试条制备方法,属于生物医药技术领域。
背景技术
皮肤癣菌病是高发性感染性皮肤病,在健康人和免疫功能不全的人身上都可能发生,且复发率高;由于皮肤癣菌病的临床表现与其他皮肤疾病如皮炎、银屑病、湿疹等的临床表现有时相似,特别是对于免疫功能不全的人的检测会更加困难;现有的皮肤癣菌检测方式比较复杂,不适合居家自用,导致个人检测成本较高,费时费力。
发明内容
针对上述存在的技术问题,本发明的目的是:提出了一种家用快速检测皮肤癣菌的测试条制备方法。
本发明的技术解决方案是这样实现的:一种家用快速检测皮肤癣菌的测试条制备方法,包含以下步骤:
①获得皮肤癣菌抗原;
②制备皮肤癣菌单抗;
③制备抗原抗体反应的胶体金试剂条;
④制备临床样品处理液;
⑤配备完整的试剂条套装及使用说明书。
优选的,所述的步骤①中,获得皮肤癣菌抗原的过程如下:
取皮肤癣菌接种至培养基内,待成长后将皮肤癣菌在无菌环境下清洗并灭活,之后将皮肤癣菌干燥,并研成粉末,去脂后用碱性提取液提取,并用滤纸过滤,检测校正PH值,然后进行无菌过滤和检定,再接种在培养基内培养,最终得到皮肤癣菌抗原。
优选的,所述的步骤③中,胶体金试剂条包含支撑垫和设置在支撑垫上的样品垫、胶金垫、NC膜和吸水垫;
样品垫用于减缓样品渗透速度,去除样品杂质,调节样品PH值和粘度;
胶金垫用于吸附金标结合物颗粒,吸附并持续将样品转移到NC膜上,并保持金标结合物颗粒的稳定性,保证金标结合物颗粒定量完全释放;
NC膜上设置有检测线和质控线,样品在NC膜上流动并与试剂混合发生免疫反应,反应在NC膜上显色;
吸水垫用于控制样品流速,促进虹吸作用。
由于上述技术方案的运用,本发明与现有技术相比具有下列优点:
本发明采用胶体金试剂条的方式来快速检测皮肤癣菌,适合于家用或基层单位使用,自检是否患了脚气或灰指甲,然后再自行去药店或网上购买抗真菌药物,可有效减少浅部真菌感染的误诊误治。
具体实施方式
下面结合实施例来说明本发明。
本发明所述的一种家用快速检测皮肤癣菌的测试条制备方法,包含以下步骤:
①获得皮肤癣菌通用、特异抗原;取皮肤癣菌(红色毛癣菌)接种至培养基内,待成长后将皮肤癣菌在无菌环境下清洗并灭活,之后将皮肤癣菌干燥,并研成粉末,去脂后用碱性提取液提取,并用滤纸过滤,检测校正PH值,然后进行无菌过滤和检定,再接种在培养基内培养,最终得到皮肤癣菌抗原。
②制备皮肤癣菌单抗;
查文献附上标准的单抗制备流程,该单抗制备后经过与常见的除红色毛癣菌以外的其他种常见皮肤癣菌进行抗原抗体反应,以验证其属特异性。
③制备抗原抗体反应的胶体金试剂条;
胶体金试剂条包含支撑垫和设置在支撑垫上的样品垫、胶金垫、NC膜和吸水垫;
样品垫用于减缓样品渗透速度,去除样品杂质,调节样品PH值和粘度;
胶金垫用于吸附金标结合物颗粒,吸附并持续将样品转移到NC膜上,并保持金标结合物颗粒的稳定性,保证金标结合物颗粒定量完全释放;
NC膜上设置有检测线和质控线,样品在NC膜上流动并与试剂混合发生免疫反应,反应在NC膜上显色;
吸水垫用于控制样品流速,促进虹吸作用。
④制备临床样品处理液;
⑤配备完整的试剂条套装及使用说明书,然后装盒包装,成为适用于家用的自行诊断检测产品。
上述实施例只为说明本发明的技术构思及特点,其目的在于让熟悉此项技术的人士能够了解本发明的内容并加以实施,并不能以此限制本发明的保护范围,凡根据本发明精神实质所作的等效变化或修饰,都应涵盖在本发明的保护范围内。
Claims (3)
1.一种家用快速检测皮肤癣菌的测试条制备方法,其特征在于,包含以下步骤:
①获得皮肤癣菌抗原;
②制备皮肤癣菌单抗;
③制备抗原抗体反应的胶体金试剂条;
④制备临床样品处理液;
⑤配备完整的试剂条套装及使用说明书。
2.根据权利要求1所述的家用快速检测皮肤癣菌的测试条制备方法,其特征在于:
所述的步骤①中,获得皮肤癣菌抗原的过程如下:
取皮肤癣菌接种至沙氏培养基内恒温培养箱内27℃培养,待成长后将皮肤癣菌菌落在无菌环境下清洗并灭活,之后将皮肤癣菌干燥,并研成粉末,去脂后用碱性提取液提取,并用滤纸过滤,检测校正PH值,然后进行无菌过滤和检定,再接种在培养基内培养,最终得到皮肤癣菌抗原。
3.根据权利要求1所述的家用快速检测皮肤癣菌的测试条制备方法,其特征在于:
所述的步骤③中,胶体金试剂条包含支撑垫和设置在支撑垫上的样品垫、胶金垫、NC膜和吸水垫;
样品垫用于减缓样品渗透速度,去除样品杂质,调节样品PH值和粘度;
胶金垫用于吸附金标结合物颗粒,吸附并持续将样品转移到NC膜上,并保持金标结合物颗粒的稳定性,保证金标结合物颗粒定量完全释放;
NC膜上设置有检测线和质控线,样品在NC膜上流动并与试剂混合发生免疫反应,反应在NC膜上显色;
吸水垫用于控制样品流速,促进虹吸作用。
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