CN111955624A - Taiwan loach feed additive and preparation method and application thereof - Google Patents
Taiwan loach feed additive and preparation method and application thereof Download PDFInfo
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- 241000252185 Cobitidae Species 0.000 title claims abstract description 62
- 239000003674 animal food additive Substances 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims abstract description 38
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 36
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 36
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000208689 Eucommia ulmoides Species 0.000 claims abstract description 20
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims abstract description 20
- 229960003237 betaine Drugs 0.000 claims abstract description 20
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000019192 riboflavin Nutrition 0.000 claims abstract description 19
- 229960002477 riboflavin Drugs 0.000 claims abstract description 19
- 239000002151 riboflavin Substances 0.000 claims abstract description 19
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 claims abstract description 18
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 18
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 18
- 235000010081 allicin Nutrition 0.000 claims abstract description 18
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 claims abstract description 18
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 18
- 239000011718 vitamin C Substances 0.000 claims abstract description 18
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 18
- 229940046009 vitamin E Drugs 0.000 claims abstract description 18
- 239000011709 vitamin E Substances 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims 1
- 241000251468 Actinopterygii Species 0.000 abstract description 31
- 235000013372 meat Nutrition 0.000 abstract description 14
- 150000001413 amino acids Chemical class 0.000 abstract description 10
- 239000000796 flavoring agent Substances 0.000 abstract description 9
- 235000019634 flavors Nutrition 0.000 abstract description 9
- 230000036039 immunity Effects 0.000 abstract description 7
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 210000003205 muscle Anatomy 0.000 abstract description 5
- 235000019640 taste Nutrition 0.000 abstract description 5
- 229940088594 vitamin Drugs 0.000 abstract description 4
- 229930003231 vitamin Natural products 0.000 abstract description 4
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- 239000011782 vitamin Substances 0.000 abstract description 4
- 229930013930 alkaloid Natural products 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 241000411851 herbal medicine Species 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 235000001014 amino acid Nutrition 0.000 description 9
- 239000000654 additive Substances 0.000 description 7
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 235000019784 crude fat Nutrition 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
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- 230000004083 survival effect Effects 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000024203 complement activation Effects 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 230000019254 respiratory burst Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108010028690 Fish Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000294599 Paramisgurnus dabryanus Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 230000022558 protein metabolic process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a Taiwan loach feed additive which comprises the following components in parts by weight: 25-35 parts of allicin, 8-12 parts of vitamin C, 8-12 parts of vitamin E, 10-15 parts of betaine, 30-40 parts of eucommia ulmoides extract and 3-6 parts of riboflavin; the invention also discloses a preparation method of the feed additive and application of the feed additive in Taiwan loach feed. The feed additive disclosed by the invention can obviously improve the content of flavor amino acid in the fish body, improve the protein and fat content of the fish body, improve the water retention and chewiness of fish body muscles and improve the fish meat quality and taste by adding various green and safe substances such as vitamins, alkaloids and Chinese herbal medicines; but also can improve the immunity of the fish body, improve the breeding success rate, and has the advantages of safe use, low cost, environmental protection and the like.
Description
Technical Field
The invention relates to the technical field of aquatic fish feed additives, and particularly relates to a Taiwan loach feed additive and a preparation method and application thereof.
Background
One of Taiwan loaches and Paramisgurnus dabryanus belongs to the order Cyprinidae, the genus Paramisguria, is freshwater fish which is omnivorous in meat, and is widely distributed in China. Loaches called 'underwater ginseng' have high medical value and nutritional value and are one of the traditional freshwater economic fishes in China. Since the last 90 s, Taiwan loaches are bred and raised successfully, and are popularized nationwide in 2000, the loach industry with annual culture quantity close to 20 ten thousand tons is formed in China, and the cultured products are exported to Korea and other countries. The Taiwan loaches grow fast and have strong disease resistance, so that the popularization and cultivation are accelerated in all parts of the country in recent years.
With the popularization of the breeding of the Taiwan loaches, the special compound feed for the loaches is also researched, developed, popularized and applied. With the maturity of the special compound feed technology for loaches, the used types of substitute raw materials are more and more, the substitute proportion is higher and higher, but the research and development progress of the premix is still in a standstill, so that the feed effect is poorer and poorer, on one hand, the immunity of fish bodies is low, on the other hand, the flavor, taste and nutritional value of fish meat are also reduced, and the commodity value and the popularization progress of the special compound feed for loaches are severely restricted. The existing research shows that the main reason for the deterioration of fish immunity, fish flavor, taste and nutritional value is the reduction of fish protein level, amino acid composition and flavor substances, the research on loach meat quality influencing factors and improving measures in the industry at present is not deep enough, few documents and reports exist, and an effective method for improving the conditions is not available.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a Taiwan loach feed additive, a preparation method and an application thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
the Taiwan loach feed additive comprises the following components: allicin, vitamin C, vitamin E, betaine, eucommia ulmoides extract and riboflavin.
Wherein the allicin is volatile oily substance extracted from Bulbus Allii bulb, and can improve meat quality of aquatic animals by long-term feeding.
The vitamin C and the vitamin E are vitamins necessary for the fish body, and can obviously increase the content of the delicious amino acid in the fish body.
The betaine can promote fat and protein metabolism, regulate fish nutritional structure, and remarkably improve fish growth performance and meat quality.
The eucommia ulmoides extract can improve the content of collagen, reduce the fat content and the diameter of muscle fiber, and improve the taste of fish muscle.
The riboflavin is an essential nutrient for maintaining normal growth and physiological metabolism of the fish, can improve the water retention and chewiness of fish muscles, and improves the meat quality and mouthfeel.
The feed provided by the invention can improve the meat quality mainly by improving the fish metabolism and enhancing the fish immunity, wherein the allicin, the vitamin and the eucommia ulmoides extract have the functions of sterilizing, disinfecting, protecting the liver and resisting stress, and the betaine and the riboflavin can obviously improve the fish metabolism process and optimize the fish nutritional structure.
As a preferred technical scheme, the weight portions of the components are as follows: 25-35 parts of allicin, 8-12 parts of vitamin C, 8-12 parts of vitamin E, 10-15 parts of betaine, 30-40 parts of eucommia ulmoides extract and 3-6 parts of riboflavin.
The feed is designed by combining the accumulated research and development results and matching the requirements of the loaches, and the usage amount of the eucommia ulmoides extract and the betaine is properly increased in the feed to achieve the technical effect of the feed.
As a preferred technical scheme, the weight portions of the components are as follows: 30-35 parts of allicin, 9-10 parts of vitamin C, 9-10 parts of vitamin E, 12-14 parts of betaine, 36-38 parts of eucommia ulmoides extract and 4-5 parts of riboflavin.
As a preferred technical scheme, the weight portions of the components are as follows: 32 parts of allicin, 10 parts of vitamin C, 10 parts of vitamin E, 12 parts of betaine, 30 parts of eucommia ulmoides extract and 6 parts of riboflavin.
As a preferred technical scheme, the weight portions of the components are as follows: 25 parts of allicin, 12 parts of vitamin C, 12 parts of vitamin E, 12 parts of betaine, 35 parts of eucommia ulmoides extract and 4 parts of riboflavin.
The second aspect of the invention provides a preparation method of the Taiwan loach feed additive, which comprises the following steps:
1) weighing the raw materials of the components in parts by weight, gradually adding the raw materials from small to large in parts by weight, mixing the raw materials after adding each material, mixing 500kg of the raw materials in a batch, and mixing for 160-180 s to obtain a mixture;
because the addition of each component in the feed is relatively large, the components with relatively small addition are preferentially mixed uniformly, and the components with relatively large addition are gradually added, so that the mixing uniformity of the whole material can be improved.
2) Crushing the mixture obtained in the step 1) to 150-200 meshes to obtain the Taiwan loach feed additive.
The third aspect of the invention provides the application of the Taiwan loach feed additive in the Taiwan loach feed.
As a preferable technical scheme, the mass ratio of the feed additive to the feed is 1: 50-1: 40.
Compared with the prior art, the invention has the following advantages:
the feed additive disclosed by the invention can obviously improve the content of flavor amino acid in the fish body, improve the protein and fat content of the fish body, improve the water retention and chewiness of fish body muscles and improve the fish meat quality and taste by adding various green and safe substances such as vitamins, alkaloids and Chinese herbal medicines; but also can improve the immunity of the fish body, improve the breeding success rate, and has the advantages of safe use, low cost, environmental protection and the like.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.
Example 1
The Taiwan loach feed additive comprises the following components in parts by weight: 25 parts of allicin, 12 parts of vitamin C, 12 parts of vitamin E, 12 parts of betaine, 35 parts of eucommia ulmoides extract and 4 parts of riboflavin.
The preparation method comprises the following steps:
1) weighing the raw materials of the components in parts by weight, gradually adding the raw materials from small to large in parts by weight, mixing the raw materials after adding each material, mixing 500kg of the raw materials in a batch, and mixing for 160-180 s to obtain a mixture;
2) crushing the mixture obtained in the step 1) to 150-200 meshes to obtain the Taiwan loach feed additive.
Example 2
The Taiwan loach feed additive comprises the following components in parts by weight: 30 parts of allicin, 8 parts of vitamin C, 10 parts of vitamin E, 10 parts of betaine, 37 parts of eucommia ulmoides extract and 5 parts of riboflavin.
The preparation method is the same as example 1 and is not repeated herein.
Example 3
The Taiwan loach feed additive comprises the following components in parts by weight: 26 parts of allicin, 10 parts of vitamin C, 8 parts of vitamin E, 13 parts of betaine, 40 parts of eucommia ulmoides extract and 3 parts of riboflavin.
The preparation method is the same as example 1 and is not repeated herein.
Example 4
The Taiwan loach feed additive comprises the following components in parts by weight: 32 parts of allicin, 10 parts of vitamin C, 10 parts of vitamin E, 12 parts of betaine, 36 parts of eucommia ulmoides extract and 4 parts of riboflavin.
The preparation method is the same as example 1 and is not repeated herein.
Example 5
The Taiwan loach feed additive comprises the following components in parts by weight: 35 parts of allicin, 8 parts of vitamin C, 8 parts of vitamin E, 10 parts of betaine, 35 parts of eucommia ulmoides extract and 4 parts of riboflavin.
The preparation method is the same as example 1 and is not repeated herein.
Example 6
The Taiwan loach feed additive comprises the following components in parts by weight: 25 parts of allicin, 10 parts of vitamin C, 10 parts of vitamin E, 15 parts of betaine, 35 parts of eucommia ulmoides extract and 5 parts of riboflavin.
The preparation method is the same as example 1 and is not repeated herein.
Test example 1
The test time is from 6 months and 5 days to 8 months and 20 days in 2017, and is carried out in Liyang city breeding base, Jiangsu province. The test was carried out in a glass fiber reinforced plastic water tank (specification: 50 cm. times.35 cm. times.40 cm) with tap water, which was sufficiently aerated to remove chlorine, as a circulating water source, and with dissolved oxygen in the water kept at 5.0mg/L and a temperature kept at 22. + -. 1 ℃. The test fish (Taiwan loach) were randomly divided into 7 groups, including 6 test groups and 1 control group. Each group is provided with 6 water tanks, and 60 Taiwan loaches are placed in each water tank. Feeding the 6 test groups with the feed added with the additive of the embodiment 1-6 respectively, wherein the test groups are 1-6 respectively; the control group was fed with the feed without any of the additives of examples 1 to 6. Feeding twice a day for each group, wherein the feeding time is 7:00 and 19:00, the feeding rate of each group is kept consistent, the feeding rate is adjusted according to the specification, and feeding is carried out by taking full feeding. After the test is finished, various indexes such as crude protein, crude fat, collagen content and the like of the Taiwan loach are detected (the result is shown in the following table 1), and the influence of the Taiwan loach feed additive on the meat quality of the Taiwan loach is researched.
TABLE 1
Note: flavor amino acids include aspartic acid, glutamic acid, glycine and alanine, and the content is the sum of the amino acids.
The test results show that the indexes of the test groups 1-6 are superior to those of the control group except that the crude fat level is not obviously different from that of the control group. Crude protein level, collagen content, pH of each test group45minThe calcium content, hardness and total flavor amino acid content of the muscle are all higher than those of the control group. The drip loss was lower in each test group than in the control group. The Taiwan loach feed additive can obviously improve the meat quality of the Taiwan loachOverall, test group 4 (fed the feed supplemented with the additive of example 4) had the best results.
Test example 2
The test time is from 2018, 5 and 29 days to 8 and 15 days, and is carried out in Zhou Shanzhen of Gaoyou city in Jiangsu province. Respectively selecting 34 Taiwan loach ponds to feed the feeds added with the additives in the embodiments 3 and 5, and respectively using the feeds as a test group 1 and a test group 2; and feeding the feed which does not contain the additive into a 34-mouth Taiwan loach pond to serve as a control group. Feeding twice a day for each group, wherein the feeding time is 7:00 and 19:00, the feeding rate of each group is kept consistent, the feeding rate is adjusted according to the specification, and feeding is carried out by taking full feeding. After the test is finished, various indexes such as crude protein, crude fat, collagen content and the like of the Taiwan loach are detected (the result is shown in the following table 2), and the influence of the Taiwan loach feed additive on the meat quality of the Taiwan loach is researched.
TABLE 2
Note: flavor amino acids include aspartic acid, glutamic acid, glycine and alanine, and the content is the sum of the amino acids.
The test results show that the fish body crude fat levels of the test group 1 and the test group 2 are not obviously different from those of the control group, but the fish body crude protein level, the collagen content and the pH value are45minMuscle calcium content, hardness and total flavor amino acids were all significantly higher than the control group, while drip loss was significantly lower than the control group. The Taiwan loach feed additive can obviously improve the meat quality of the Taiwan loach.
Test example 3
The same tests as in test example 2 were carried out on the taiwan loach feed additives of example 1, example 2, example 4 and example 6, respectively, to obtain the above-mentioned advantageous effects, i.e., to remarkably improve the meat quality of the taiwan loach.
Test example 4
The test time is 6 months and 15 days to 8 months and 30 days in 2019, and is carried out in Zhou Shanzhen city of Gaoyou, Jiangsu province. Respectively selecting 20 Taiwan loaches to feed the feed added with the additives in the embodiments 1, 3 and 5, and respectively using the feed as a test group 1, a test group 2 and a test group 3; and feeding the feed which does not contain the additive into a 20-mouth Taiwan loach pond to serve as a control group. Feeding twice a day for each group, wherein the feeding time is 7:00 and 19:00, the feeding rate of each group is kept consistent, the feeding rate is adjusted according to the specification, and feeding is carried out by taking full feeding. After the test is finished, various indexes such as survival rate, serum lysozyme, alternative complement activity, respiratory burst activity and the like of the Taiwan loach are detected (the result is shown in the following table 3), and the influence of the Taiwan loach feed additive on immunity and survival rate of the Taiwan loach is researched.
TABLE 3
The test result shows that the lysozyme level, the alternative complement activity and the respiratory burst activity of the test group are all higher than those of the control group, and the survival rate is also obviously higher than that of the control group. The Taiwan loach feed additive can obviously improve the immunity and the breeding success rate of the Taiwan loach.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.
Claims (8)
1. The Taiwan loach feed additive is characterized by comprising the following components: allicin, vitamin C, vitamin E, betaine, eucommia ulmoides extract and riboflavin.
2. The Taiwan loach feed additive according to claim 1, wherein the Taiwan loach feed additive comprises 25 to 35 parts of garlicin, 8 to 12 parts of vitamin C, 8 to 12 parts of vitamin E, 10 to 15 parts of betaine, 30 to 40 parts of eucommia ulmoides extract and 3 to 6 parts of riboflavin.
3. The Taiwan loach feed additive according to claim 2, wherein the Taiwan loach feed additive comprises the following components in parts by weight based on the weight of air-dried substances: 30-35 parts of allicin, 9-10 parts of vitamin C, 9-10 parts of vitamin E, 12-14 parts of betaine, 36-38 parts of eucommia ulmoides extract and 4-5 parts of riboflavin.
4. The Taiwan loach feed additive according to claim 2, wherein the ingredients comprise, by weight, 32 parts of allicin, 10 parts of vitamin C, 10 parts of vitamin E, 12 parts of betaine, 30 parts of eucommia ulmoides extract and 4 parts of riboflavin.
5. The Taiwan loach feed additive according to claim 2, wherein the components are as follows in parts by weight: 25 parts of allicin, 12 parts of vitamin C, 12 parts of vitamin E, 12 parts of betaine, 35 parts of eucommia ulmoides extract and 4 parts of riboflavin.
6. The preparation method of the Taiwan loach feed additive according to any one of claims 1 to 5, comprising the steps of:
1) weighing the raw materials of the components in parts by weight, gradually adding the raw materials from small to large in parts by weight, mixing the raw materials after adding each material, mixing 500kg of the raw materials in a batch, and mixing for 160-180 s to obtain a mixture;
2) crushing the mixture obtained in the step 1) to 150-200 meshes to obtain the Taiwan loach feed additive.
7. The use of the Taiwan loach feed additive according to any one of claims 1 to 5 in Taiwan loach feed.
8. The use of claim 7, wherein the mass ratio of the feed additive to the feed is 1: 50-1: 40.
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CN202010870782.XA CN111955624A (en) | 2020-08-26 | 2020-08-26 | Taiwan loach feed additive and preparation method and application thereof |
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CN103749985A (en) * | 2014-01-28 | 2014-04-30 | 四川农业大学 | Additive for modifying meat quality of farmed fishes, application method, application and fish feed |
CN104366100A (en) * | 2014-11-10 | 2015-02-25 | 余仁忠 | Loach feed nutritional material |
CN106259093A (en) * | 2016-08-16 | 2017-01-04 | 朱德苹 | A kind of method of loach breeding |
CN109198263A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of liver and gallbladder protection feed addictive of Chinese snake head, expanded pellet diet and preparation method thereof |
CN110150511A (en) * | 2019-07-08 | 2019-08-23 | 河南牧业经济学院 | A kind of freshwater fish quality improving agent and preparation method thereof |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103749985A (en) * | 2014-01-28 | 2014-04-30 | 四川农业大学 | Additive for modifying meat quality of farmed fishes, application method, application and fish feed |
CN104366100A (en) * | 2014-11-10 | 2015-02-25 | 余仁忠 | Loach feed nutritional material |
CN106259093A (en) * | 2016-08-16 | 2017-01-04 | 朱德苹 | A kind of method of loach breeding |
CN109198263A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of liver and gallbladder protection feed addictive of Chinese snake head, expanded pellet diet and preparation method thereof |
CN110150511A (en) * | 2019-07-08 | 2019-08-23 | 河南牧业经济学院 | A kind of freshwater fish quality improving agent and preparation method thereof |
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