CN114081015B

CN114081015B - Efficient spawning induction method for Rana spinosa

Info

Publication number: CN114081015B
Application number: CN202111620779.3A
Authority: CN
Inventors: 夏斌; 兰珍群; 涂建国; 吴涛
Original assignee: Jiangxi Yangjifeng Ecological Agriculture Co ltd
Current assignee: Jiangxi Yangjifeng Ecological Agriculture Co ltd
Priority date: 2021-12-27
Filing date: 2021-12-27
Publication date: 2023-03-28
Anticipated expiration: 2041-12-27
Also published as: CN114081015A

Abstract

The invention discloses a method for efficiently inducing spawning of Rana spinosa, which comprises the steps of mixing an induced spawning feed with water, directly introducing the mixed feed into a Rana spinosa pool for feeding, mixing and feeding mixed hormone, an amino acid additive, pectin and a centella asiatica extract, reducing discomfort caused by hormone feeding to organisms, simultaneously adding seaweed meal, using polysaccharide substances and pectin in the seaweed meal together, gelatinizing the mixed hormone and nutrient substances separated out from the feed when the seaweed meal is dissolved in water, enabling the mixed hormone and the nutrient substances separated out from the feed to have better effects, simultaneously prolonging the mixed hormone and the separated nutrient substances and the effective duration, feeding the Rana spinosa by using the mixed hormone first, simulating the estrus condition of the Rana spinosa in an estrus period, simulating the freshness of the estrus period, and increasing the induced spawning rate and the spawning rate of individuals by using a direct injection method of the induced spawning hormone, and simultaneously reducing the damage of the induced spawning to the Rana spinosa.

Description

Efficient spawning induction method for Rana spinosa

Technical Field

The invention relates to the technical field of stone frog spawning induction, in particular to a stone frog high-efficiency spawning induction method.

Background

China is the first big country of world aquaculture and the only country in the world where the aquaculture yield exceeds the fishing yield, and aquaculture plays a very important role in national economy. In recent years, the aquaculture industry in China is vigorously developed, and the rapid rise of frog culture is widely concerned by aquaculture practitioners.

The stone frog is one of the main edible frogs because the stone frog has delicious meat taste and high growth speed and is large. According to the traditional Chinese medicine, the meat flavor Gan Xianping of the rana spinosa enters lung, stomach and kidney channels, has the effects of tonifying spleen, removing food retention, nourishing and strengthening, and is used for treating symptoms such as dyspepsia, anorexia and weakness.

The existing yield of the rana spinosa cannot meet the market demand, so that the market begins to use a manual intervention means to improve the yield of the rana spinosa, for example, the rana spinosa is eaten in all seasons by using an artificial spawning promotion mode, but the existing artificial spawning promotion method has poor spawning promotion effect on the rana spinosa, usually needs to use an artificial insemination mode, is complicated and troublesome, has great damage to the rana spinosa, is difficult to select the good rana spinosa to repeatedly promote spawning even in a natural insemination mode, and needs to research an induced spawning method with low damage to the rana spinosa and high return rate in order to improve the yield of the rana spinosa and take the humanistic spirit.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a method for inducing the spawning of Rana spinosa with high efficiency.

In order to solve the technical problems, the invention adopts the following technical scheme,

a method for inducing spawning of Rana spinosa with high efficiency comprises the following steps:

s1, selecting living male and female frogs with the size of more than 300g, putting the living male and female frogs into a frog pond after disinfection and cleaning are finished, and adjusting the temperature of a spawning pond to 15-20 ℃ to prepare before spawning induction;

s2, feeding a meal of induced spawning feed 7 days before induced spawning, and mixing the induced spawning feed and water according to the ratio of 1:20, and the oxytocic feed comprises 0.003-0.02 part by weight of mixed hormone, 10-20 parts by weight of bean powder, 0.1-1 part by weight of calcium fatty acid, 0.01-0.2 part by weight of amino acid additive, 0.3-0.5 part by weight of multivitamin, 10-15 parts by weight of egg yolk powder, 20-50 parts by weight of meat powder, 1-3 parts by weight of dried seaweed powder, 0.03-0.1 part by weight of pectin and 0.05-0.1 part by weight of centella extract;

s3, injecting oxytocin by using a two-time injection method for female frogs which are fed for 7 days continuously, wherein the total injection dose is 2.0-2.5ml per kilogram, and the total injection dose is 1.5-2.0ml per kilogram for male frogs in a single injection.

Preferably, the two-shot method comprises the steps of:

taking 20-30% of the total dose of the injection oxytocic dose as a first injection;

the remaining dose is injected 8-12 hours after the injection of the first needle.

Preferably, the oxytocin comprises the following components calculated by each 1ml content:

LRH-A3 0.1～2.5μg，

Dom 0.3～1.1mg，

RES 0.15～0.5mg。

preferably, the induced spawning feed further comprises the following components:

0.02-0.2 parts of 50% choline chloride-loaded carrier powder, 0.5-2 parts of Omega-6 and 0.1-0.3 parts of Omega-3.

Preferably, the preparation method of the choline chloride-loaded carrier powder comprises the following steps:

mixing the straw powder and a choline chloride aqueous solution in a vacuum environment, standing for 7-9 h, heating to a semi-dry state at 70-120 ℃, and drying in a roller dryer at 35-45 ℃ to obtain the choline chloride-loaded carrier powder.

Preferably, the amino acid additive is one or more of methionine, tryptophan, lysine, phenylalanine, tyrosine and valine.

Preferably, the mixed hormone is any one or more of DOM and HCG, PG and LRH-A.

Compared with the prior art, the invention has the advantages that:

1. the oxytocic feed is mixed with water and then directly introduced into a frog pond for feeding, and the mixed hormone, the amino acid additive, the pectin and the centella asiatica extract are mixed for feeding, so that the discomfort caused by hormone feeding to organisms can be reduced, the seaweed meal is added, the polysaccharide substance and the pectin in the seaweed meal are used together, the mixed hormone and the nutrient substances precipitated in the feed can be gelatinized when the seaweed meal is dissolved in water, the effect is better, and the freshness and the effective duration of the mixed hormone and the precipitated nutrient substances can be prolonged;

2. the method uses a mode of firstly mixing hormone for feeding and simulating estrus temperature and environment to profoundly influence the estrus condition of the frogs to be induced to spawn, and is accompanied with a method of directly injecting the induced-spawning hormone, thereby increasing the induced spawning rate and the individual spawning rate and simultaneously reducing the damage of induced spawning to the stone frogs.

Detailed Description

The invention is further described in the following description and specific examples, but the scope of the invention is not limited thereby.

Example 1

s1, selecting living male and female frogs with more than 300g of physique, putting the living male and female frogs into a frog pond after disinfection and cleaning are completed, and adjusting the temperature of the spawning pond to 18 ℃ to prepare before spawning induction;

s2, feeding a meal of induced spawning feed 7 days before induced spawning, and mixing the induced spawning feed and water according to the ratio of 1:20, and the hasten parturition feed comprises, by weight, 0.008 parts of mixed hormone, 15 parts of bean powder, 0.2 parts of calcium fatty acid, 0.05 parts of amino acid additive, 0.4 parts of compound vitamin, 12 parts of egg yolk powder, 40 parts of meat powder, 2 parts of dried seaweed powder, 0.06 parts of pectin and 0.07 parts of centella extract;

s3, injecting oxytocin by using a two-time injection method for female frogs after being continuously fed for 7 days, wherein the total injection dose is 2.2ml per kilogram, and the total injection dose is 1.8ml per kilogram for male frogs in a single injection.

In this example, the two injections were:

using 30% of the total dose of oxytocin as a first injection;

injecting the remaining dose of oxytocin 10 hours after the injection of the first needle;

in this example, the oxytocin is:

LRH-A3 0.2μg，

Dom 0.6mg；

in this example, the amino acid additives were 65% lysine, 13% valine, 12% phenylalanine, 10% tyrosine.

Comparative example 1

This comparative example differs from example 1 in that:

in step S2, the oxytocic feed is 0.008 parts of mixed hormone, 15 parts of bean powder, 0.2 parts of fatty acid calcium, 0.05 parts of amino acid additive, 0.4 parts of multivitamin, 12 parts of egg yolk powder, 40 parts of meat powder and 2 parts of dried seaweed powder.

Example 2:

this example differs from example 1 in that:

the two-shot step in step S3 includes:

using 25% of the total dose of oxytocin as a first injection;

the remaining dose of the oxytocin was injected 10 hours after the injection of the first needle.

Comparative example 2

This comparative example differs from example 2 in that:

at the time of two injections in step S3, 40% of the total amount of the oxytocin injected was taken as the first injection.

Example 3

The present embodiment 3 differs from embodiment 2 in that:

the composition of oxytocin in this example, calculated per 1ml, comprises the following components:

LRH-A3 0.2μg，

Dom 0.6mg，

RES 0.3mg。

comparative example 3

This comparative example differs from example 3 in that:

100IU HCG is also added into the oxytocin according to the content of each 1 ml.

Comparative example 4

This comparative example differs from example 3 in that:

the oxytocin contains LRH-A30.2 μ g, dom0.6mg and HCG100IU calculated by per 1ml content.

Comparative example 5

This comparative example differs from example 3 in that:

the oxytocin contains LRH-A30.2 μ g, HCG100IU and RES 0.3mg per 1 ml.

Example 4

This example differs from example 3 in that:

in the embodiment, the ingredients of the induced spawning feed are optimized, and the induced spawning feed further comprises the following ingredients:

0.06 parts of 50% choline chloride-loaded carrier powder, 0.15 parts of Omega-6, 0.2 parts of Omega-3;

at the same time, a process for the preparation of a choline chloride-loaded carrier powder is defined, which comprises the steps of:

mixing the straw powder and a choline chloride aqueous solution in a vacuum environment, standing for 8h, heating to a semi-dry state at 80 ℃, and drying in a roller dryer at 40 ℃ to obtain choline chloride-loaded carrier powder.

Comparative example 6

The present embodiment differs from embodiment 4 in that:

the oxytocic feed also comprises 0.06 part of 50% choline chloride loaded carrier powder.

Example 5

This example differs from example 4 in that:

the amino acid additives in step S2 in this example were 76% lysine, 13% valine, 6% phenylalanine, 5% tyrosine.

Comparative example 7

This comparative example differs from example 5 in that:

the amino acid additive in step S2 in this example was 76% lysine, 4% methionine, 11% phenylalanine, 9% tyrosine.

Comparative example 8

This comparative example differs from example 5 in that:

the amino acid additives in step S2 in this example were 76% lysine, 13% phenylalanine, 11% tyrosine.

Example 6

This example differs from example 5 in that:

the mixed hormones in step S2 in this example were a composition of 35% Dom and 40% PG, 25% LRH-A.

Comparative example 9

This comparative example differs from example 6 in that:

the mixed hormones in step S2 is a composition of 35% Dom and 40% HCG, 25% LRH-A.

Comparative example 10

Unlike example 6, the mixed hormones in step S2 in this example were a composition of 35% Dom and 30% PG, 20% LRH-A, 15% HCG.

Comparative example 11

This comparative example differs from example 6 in that:

the mixed hormones in step S2 is a composition of 35% Dom and 40% HCG, 25% LRH-A3.

Comparative example 12

Unlike example 6, the induced spawning body was replaced with a salamander.

The spirit states of the rana chensinensis and the salamander of comparative example 12 were observed in five days after induction of the induced labor of the rana chensinensis and the salamander by using the methods of comparative examples 1, 6, 7 and 8 and examples 1, 4 and 5, respectively, and the specific results are shown in table 1:

TABLE 1

Referring to table 1, when 76% of lysine, 13% of valine, 6% of phenylalanine and 5% of tyrosine are used as amino acid additives as the oxytocic feed, the injury to female and male rana spinosa is the lowest, and the recovery after the oxytocic is the best can be seen in table 1;

the stone frogs were naturally bred using the induction method of comparative examples 1, 2, 3, 4, 5, 7, 8, 9, 10 and examples 1, 2, 3, 5, 6, respectively, and the salamander was naturally bred using the method of comparative example 12, with the specific results shown in table 2:

TABLE 2

	Success rate of induced spawning (female)	Success rate of induced spawning (male)	Individual egg laying rate
				Comparative example 1	55％	68％	69.01％
Comparative example 2	52％	64％	60.72％
				Comparative example 3	60％	71％	71.29％
Comparative example 4	59％	67％	70.54％
				Comparative example 5	57％	66％	70.06％
Comparative example 7	80％	90％	89.19％
				Comparative example 8	81％	92％	84.27％
Comparative example 9	90％	94％	88.94％
				Comparative example 10	91％	94％	88.46％
Comparative example 11	71％	81％	70.29％
				Example 1	72％	81％	81.03％
Example 2	76％	86％	83.22％
				Example 3	82％	90％	83.69％
Example 5	90％	95％	88.46％
				Example 6	94％	96％	90.33％
Comparative example 12	70％	78％	76.84％

Referring to tables 1-2, and the contents of the above comparative examples and examples, it can be seen that the addition of pectin and centella asiatica extract has a decisive effect on the success rate of induced spawning and the individual spawning rate, and simultaneously has a good effect on protecting the body of rana spinosa during induced spawning;

comparing example 2 with comparative example 2, it can be seen that when the first injection dosage is less than 30%, the success rate of induced spawning and the individual spawning rate are higher;

comparing example 3 with comparative examples 3-5, it can be seen that example 3 has a better spawning rate and a higher individual spawning rate than the oxytocin formulation of comparative examples 3-5, and at the same time, increasing HCG does not lead to a higher spawning rate and spawning rate, or even to an excessive hormone usage;

comparing example 4 with comparative example 6, it is known that addition of Omega-6 and Omega-3 can compensate for the problem of improper use of amino acids;

comparing example 5 with comparative examples 7 to 8, it is known that when 76% lysine, 13% valine, 6% phenylalanine, 5% tyrosine are used as amino acid additives as the oxytocic feed, the harm to female and male rana spinosa is the lowest, and the optimal ratio of the amino acid additives is the oxytocic feed, and that the addition of methionine can increase the individual egg laying rate, but the harm to rana spinosa is extremely large;

comparing example 6 with comparative examples 9-11, it can be seen that the spawning induction success rate of the matched female and male frogs with 35% of Dom, 40% of PG and 25% of LRH-A is the best, the individual spawning rate is the highest, and meanwhile, the effect of replacing LRH-A with homologous LRH-A3 is reduced greatly;

from the comparative example 12, it can be known that the induced spawning method used by the invention has the best effect on the rana spinosa, and the optimal proportion cannot become the best induced spawning method when the induced spawning method is used as a salamander of amphibians;

as can be seen from the above, in example 6, the damage to the rana spinosa is the lowest, the recovery effect after induced spawning is the best, the induced spawning effect of both male and female frogs is the best, and the individual spawning rate is also the highest, so that example 6 is considered as the most preferable example of the present invention.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-described embodiments. All technical schemes belonging to the idea of the invention belong to the protection scope of the invention. It should be noted that modifications and embellishments within the scope of the invention may be made by those skilled in the art without departing from the principle of the invention, and such modifications and embellishments should also be considered as within the scope of the invention.

Claims

1. A method for inducing spawning of Rana spinosa with high efficiency is characterized by comprising the following steps:

s2, feeding a meal of induced spawning feed 7 days before induced spawning, and mixing the induced spawning feed and water according to the ratio of 1:20, and the induced spawning feed comprises, by weight, 0.003-0.02 part of mixed hormone, 10-20 parts of bean powder, 0.1-1 part of calcium fatty acid, 0.01-0.2 part of an amino acid additive, 0.3-0.5 part of multivitamin, 10-15 parts of egg yolk powder, 20-50 parts of meat powder, 1-3 parts of dried seaweed powder, 0.03-0.1 part of pectin and 0.05-0.1 part of centella asiatica extract, wherein the amino acid additive is a combination of lysine, phenylalanine, tyrosine and valine;

2. The method for inducing spawning of Rana spinosa with high efficiency as claimed in claim 1, wherein the two-time injection method comprises the following steps:

3. The method for inducing the spawning of the Rana spinosa with high efficiency as claimed in claim 1, wherein the oxytocin comprises the following components by per 1 ml:

LRH-A3 0.1～2.5mg，

Dom 0.3～1.1mg，

RES 0.15～0.5mg。

4. the method for inducing the spawning of the Rana spinosa efficiently as claimed in claim 1, wherein the induced spawning feed further comprises the following components:

0.02-0.2 parts of 50% choline chloride-loaded carrier powder, 0.5-2 parts of Omega-6, and 0.1-0.3 parts of Omega-3.

5. The method for inducing spawning of Rana spinosa with high efficiency as claimed in claim 4, wherein the method for preparing the choline chloride-loaded carrier powder comprises the following steps:

mixing the straw powder and a choline chloride aqueous solution in a vacuum environment, standing for 7-9 h, heating to a semi-dry state at 70-120 ℃, and drying in a roller dryer at 35-45 ℃ to obtain choline chloride-loaded carrier powder.

6. The method for inducing spawning of Rana spinosa with high efficiency as claimed in claim 1, wherein: the mixed hormone is any one or combination of DOM, HCG, PG and LRH-A.