CN111681707A - Method for evaluating temperature and humidity state of growing environment of nursery pig individual based on nasal cavity eukaryotic microorganism relative abundance - Google Patents
Method for evaluating temperature and humidity state of growing environment of nursery pig individual based on nasal cavity eukaryotic microorganism relative abundance Download PDFInfo
- Publication number
- CN111681707A CN111681707A CN202010158494.1A CN202010158494A CN111681707A CN 111681707 A CN111681707 A CN 111681707A CN 202010158494 A CN202010158494 A CN 202010158494A CN 111681707 A CN111681707 A CN 111681707A
- Authority
- CN
- China
- Prior art keywords
- species
- temperature
- nursery pig
- nasal cavity
- relative abundance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Theoretical Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Data Mining & Analysis (AREA)
- Evolutionary Biology (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Pure & Applied Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Mathematical Physics (AREA)
- Computational Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Mathematical Optimization (AREA)
- Mathematical Analysis (AREA)
- Probability & Statistics with Applications (AREA)
- Software Systems (AREA)
- Physiology (AREA)
- Databases & Information Systems (AREA)
- Algebra (AREA)
- Operations Research (AREA)
- General Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for evaluating the temperature and humidity state of a growing environment of a nursery pig individual based on the relative abundance of eukaryotic microbes in a nasal cavity.
Description
Technical Field
The invention belongs to the technical field of live pig breeding environment evaluation, and particularly relates to a method for evaluating the temperature and humidity state of a growing environment of a nursery pig individual based on the relative abundance of eukaryon microorganisms in a nasal cavity.
Background
The breeding environment is one of the factors determining the breeding productivity of the live pigs, the contribution rate to the breeding of the live pigs is up to 25 percent, and the improvement of the breeding environment is a necessary means for improving the breeding productivity of the live pigs. The temperature and humidity state is the most important part of the culture environment and can be represented by a temperature and humidity index. The warm and humid environment can affect the breeding productivity of live pigs from multiple angles, the immunity of the live pigs is reduced in the warm and humid environment of cold stress and heat stress, the fat deposition of the live pigs is reduced in the meat quality at high temperature, and the live pigs in the warm and humid state area of the uncomfortable environment need to consume more energy to maintain constant body temperature, so that the feed conversion rate is reduced. The existing equipment for adjusting the temperature and humidity environment for live pig breeding in a farm is provided with a water curtain fan cooling system and a heat preservation lamp fan heater heat preservation system, a heat preservation lamp capable of adjusting power and heat production quantity is produced, an accurate breeding concept is also provided, and accurate environment control is part of accurate breeding.
The accurate evaluation of live pig breeding environment is a prerequisite condition for accurate environment control, and the warm and humid environment part of the environment evaluation in the live pig breeding process is generally completed by a thermometer and a hygrometer at present. Workers use a thermometer and a hygrometer to measure the temperature and the humidity of the pigsty, and then calculate the temperature and humidity index by using a temperature and humidity index calculation formula to evaluate the temperature and humidity environment of the breeding house, wherein the temperature and humidity index can be calculated by any two values of dry bulb temperature (Td, DEG C), wet bulb temperature (Tw, DEG C), dew point (Tdp, DEG C) and relative humidity (RH, percent), and one of the following calculation formulas is selected for calculation: THI ═ Td +0.36Tdp +41.2 or THI ═ 0.81Td + (0.99Td-14.3) RH +46.3 or THI ═ 0.72(Td + Tw) + 40.6. The method can judge the temperature and humidity environment state of the breeding house at a certain time point, but the temperature and humidity environment state of the live pigs in a period of time cannot be accurately evaluated. The temperature and humidity state of the breeding house generally changes dynamically along with the change of time, and the temperature and humidity state of different areas of the same animal house also differs, so that the temperature and humidity indexes measured and calculated by a thermometer and a hygrometer at fixed time and fixed points are difficult to evaluate the temperature and humidity state of live pigs moving in a certain area within a period of time. The environmental temperature and humidity state can affect the growth of eukaryotic microorganisms, which are different from different types and levels of eukaryotic microorganisms, and the environmental temperature and humidity state can affect the growth of genus level eukaryotic microorganisms, which are different from different types of eukaryotic microorganisms, the optimal temperature condition for the growth of Saccharomyces (Saccharomyces) is 20-30 ℃, the optimal growth temperature of Fusarium (Fusarium) can grow under the condition of 1-39 ℃ but is 25-30 ℃, the optimal growth temperature of Rhizopus (Rhizopus) is higher than 30-37 ℃, and the heat resistance of Candida (Candida) is not strong. Research has shown that in winter, the indoor temperature of northern parts with warm air is too high, but the relative humidity is low, so that a large number of pathogenic bacteria grow in the respiratory tract.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for evaluating the temperature and humidity state of the growing environment of a nursery pig individual based on the relative abundance of eukaryotic microbes in the nasal cavity.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the method for evaluating the temperature and humidity state of the growing environment of the nursery pig individual based on the relative abundance of the eukaryotic microbes in the nasal cavity comprises the steps of simultaneously and quantitatively detecting the relative abundance of the eukaryotic microbes in the nasal cavity of the nursery pig relative to total eukaryotic microbes in the nasal cavity, and evaluating the temperature and humidity state of the growing environment of the nursery pig by using the eukaryotic microbes or the combination thereof, wherein the eukaryotic microbes comprise: arthropoda phylum; liliopsida, Saccharomyces (Saccharomyces), oligohymenomycetes (Oligohymenophora), Chrysophyceae (Chrysophyceae), Chromorpha (Chromadorea), Chaetomium (Sordariomycetes), Tremellaceae (Tremellomycetes), Pezizomycetes (Pezizomycetes), Arachnida, Coccomyycetes (Trebouxiophyceae), Mononidae (monolonta), Chlorophycetes (Chlorophyceae), Malassiomycetes, Cryptophyceae (Cryptophyceae), Trichosporoides (Spirotria), Hydrozoa (Hyzoea); poales, Saccharomyces (Saccharomyces), Dimethylgentida (Diplophores), Hypocreales (Hypocrea), Trichosporonales, Neocallimastigales, Theales (Lecanorales), Eurotiales (Eurotiales), Brassicales, Rosales, Chlamydomonadales, Malassezia (Malasseziales), Solanales, Filobasidiales (Filobasidiales), Stolidocarchiales, Fagaleles, Cyclophytales (Sphaerpales), watchcase (Arcelllinida), Tremellales (Tremellales), Fucales (Ulotriles), Pyramodiales, Hymenochaetales (Hypocreales), Hypocreales (Ochrotides); saccharomycetaceae (Saccharomyces cerevisiae), Trichosporon aceae, Erythroxylaceae (Nectriaceae), Malasseziaceae, Phaffoylaceae (Hymenochaetaceae), Mrakiaceae, Lichtheimaceae, Rhizophyllaceae; zea, Zymomonas (Kazachstania), Trichosporon (Ochromonas), Scorzonera (Sarocladium), Bromeliothrix, Debaryomyces (Debaryomyces), Malassezia (Malassezia), Malus, Rhizopus (Rhizopus), Naganishia, Candida, Sterkiella, Hemiptelelea, Spermartissella, Rhizomucor (Rhizomucor), Chlamydonella, Phytophthora (Distigma), Tausonia, Hydnochaete (Hydnochaete), Tetrahymena (Tetrahymena), Oryza, Trichosporon (Trichosporon), Homusum, Arposis (Trepophyella), Phyllospora, Rhizoctonia (Rhizoctonia), and Rhizoctonia (Rhizoctonia); zea _ mays species, Kazachstania _ telluris species, Tetrahymena _ corrisis species, Rhabditides _ humicola species, Blastocystis _ sp.subtype _3 species, Candida albicans (Candida _ albicans), Trebouxia _ jamesi species, Naganishia _ vischni species, Diutina _ rugosa species, Hemipherea _ davidi species, Spermanitiella _ europaea species, Bahusaka _ longispora species, Distingma _ proteus species, Taussonia _ pulullans species, Hydnochaete _ dui, Silk (Trinospora _ corefaciens), Aspergillus _ pullulan _ 1 species, Amenstalaria _ pullulans species, or Laminaria _ strain.
The method comprises the following specific steps:
(1) establishing and selecting a regression model of the relative abundance of the nasal microorganisms and the temperature and humidity index of the environment of the nursery pig reflecting the temperature and humidity environment state of the nursery pig (selection standard: R)2>0.8 requirement p relating to p value<0.05) as follows:
① class level, THI 77.04-12.85x1-82.96x2+38.29x3+93.41x4-8.59x5-562.97x6-853.91x7+772.03x8+1543.54x9-24754.73x10+1117.40x11-4959.84x12+6713.67x13+212386.71x14-3805.85x15+30401.80x16(ii) a The model is LASSO model, and coefficient R is determined2＝0.8613；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x16The relative abundance of the eukaryotic microorganisms Liliopsida, Saccharomyces, Oligochaeta, Chrysophyceae, Chromophyceae, Chaetomium, Tremellaceae, Panomycetes, Arachnida, Coccomyyceae, Mononidae, Chlorophyceae, Malasseziomyces, Cryptophyceae, Demospongiae, Hydrozoa relative to the total eukaryotic microorganisms of the nasal cavity is respectively as follows: percent;
② mesh level, THI 76.43-17.68x1-125.78x2-245.52x3-669.06x4-1091.73x5+1195.59x6-4130.28x7+3832.18x8+5401.91x9-22665.39x10-1262.74x11+6273.57x12-1213.96x13+94177.67x14-8138.89x15+778026.7x16+4940.84x17+95728.04x18-20800.44x19-104152.25x20+72337.23x21-260723.7x22+1.47x23-44706.57x24(ii) a The model is LASSO model, and coefficient R is determined2＝0.8613；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x24The relative abundance of the nursery pig nasal eukaryotic microorganisms, Poales, Saccharomycoporiales, Digastriales, Hypocrea, Trichosporonales, Neocallimastigales, Theales, Eurotiales, Brassicales, Rosales, Chlamydomonadales, Malaytales, Solanales, Nepalustaria, Stolidobrachia, Fagaales, Cyclophytales, watchcase, Tremellales, Aphyllophorales, Pyramiodales, Dermatophyllales, Filiperidiales, Cystoobasidiales, relative to the total eukaryotic nasal microorganisms, in units of: percent;
③ family level of THI 74.69403-118.91x1-114.10x2-293.41x3+577.06x4-718.67x5-130563.55x6-8165.30x7(ii) a The model is LASSO model, and coefficient R is determined2＝0.8301；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x7Relative abundance of the nursery pig nasal cavity eukaryotic microorganisms saccharomycetaceae, trichosporinaceae, erythrocasaceae, Malasseziaceae, phaffomomycetaceae, erythrochaceae, Mrakiaceae relative to nasal cavity total eukaryotic microorganisms in units of: percent;
④ genus level, THI 78.76-30.35x1-36.64x2+16.03x3-1109.55x4-2820.99x5-206.42x6+354.05x7-44705.11x8-22761.51x9+21032.98x10-35505.62x11-33058.75x12-27239.03x13-13604.5x14+165745.7x15-1.78x16-93578.76x17-41798.36x18-190048.3x19(ii) a The model is LASSO model, and coefficient R is determined2＝0.9861；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x19Respectively belong to genus Zea and genus Zymomonas (Kazac) which are eukaryotic microorganisms of nasal cavity of nursery pighstania), phaeotrichum (Ochromonas), cladocera (Sarocladium), bromelilotrix, Debaryomyces (Debaryomyces), Malassezia (Malassezia), Malus, Rhizopus (Rhizopus), Naganishia, Candida (Candida), Sterkiella, hemiptea, spenrmartissella, Rhizomucor (rhizomucocor), Chlamydonella, pleomorpha (Distigma), Tausonia, dermataceae (hydochaete) relative to the relative abundance of total eukaryotic microorganisms of the nasal cavity in units of: percent;
⑤ levels of THI 77.30-26.08x1-101.62x2+12.81x3-393.02x4+5037.59x5-2847.75x6-18478.26x7+42621.47x8-31045.96x9-49866.91x10-49842.29x11+7253.92x12-80561.46x13-17228.1x14-59962.73x15(ii) a The model is LASSO model, and coefficient R is determined2＝0.9741；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x15The relative abundance of the eukaryotic microorganisms Zea _ mays, Kazachstania _ telluris, tehymena _ corrisis, rhabdidodes _ humicola, blastystitis _ sp, subtype _3, candida albicans, Trebouxia _ jamesi, naganisia _ vismanii, Diutina _ rugosa, hemipteea _ davidi, spenrmartheiella _ europaa, bausakala _ longispora, Distigma _ proteus, Tausonia _ pullulans, hydnocharea _ duii relative to the total eukaryotic microorganisms in units of: percent;
⑥ Total THI 80.43+509.55x1-112454.19x2-150.30x3-27328.24x4+69.95x5+11819.60x6+292830.3x7+7566.22x8-657.14x9-246075.5x10+32726.74x11-5440.98x12-15061.11x13+16105.21x14-45.89x15+110.18x16-42.21x17+26.51x18-5.98x19-31.19x20-1325.87x21+782.44x22+108.99x23-40055.25x24-132290.1x25+1083.27x26+11120.24x27-44335.50x28+2783.59x29-29868.41x30-3012.42x31-137311.8x32+1149.58x33-31.01x34-3115.54x35-6.96x36-49597.7x37-3632.9x38-377.93x39-232.58x40-12.80x41+39827.06x42-1074.11x43+1585.58x44+30.82x45-1343.39x46-91516.32x47-198.74x48+59262.55x49-9029.64x50-197163.71x51(ii) a The model is LASSO model, and coefficient R is determined2＝0.9925；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x51The species are the eukaryotic microorganisms Arthropoda phylum, Euglena, Chromomycete, Coccomyxa, Zosterales, Brassicales, Fagaleles, Cyclocystiles, Rhizophylldactyles, Eurycolatoles, Anthioatheca, Aphyllophorales, Cystofilobiales, Lichtheimaceae, Rhizophyllaceae, unclassiformes, Zea, Tetrahymena, Oryza, Trichosporoides, Scopulariopsis, Hordeum, Arabis, Coccomyeria, Malus, nephrospora, Naganishia, Candida, Auciliopsis, Sterkia, Hemiptelegilia, Spermamyces, Blastomyces, Tremella, Schizosaccharomyces, Thermoascus, Schizosaccharomyces, Polyporus, Schizosaccharomyces, Thermoascus Ammopiptanthus mongolicus strainRelative abundance of total eukaryotic microbes relative to the nasal cavity in units of: percent;
(2) and quantitatively measuring the relative abundance of eukaryotic microorganisms in the nasal cavity of the nursery pig, substituting the measured result into a corresponding regression model, and calculating to obtain the temperature and humidity index of the environment in which the nursery pig is located in a short period.
Wherein the nasal cavity eukaryotic microorganism relative abundance index is obtained based on 18S rDNA sequencing of IonS5TMXL sequencing platform
The invention is further illustrated below:
the fodder and the variety in the breeding process of the nursery pig are consistent, the breathing heat measuring cabin is used for ensuring that the pig is in a stable environment temperature and humidity state and is designed repeatedly, and the fluctuation of the relative abundance of the eukaryotic microorganisms in the nasal cavity is only influenced by the environment temperature and humidity state. The invention respectively analyzes the relative abundance indexes of each nasal cavity eukaryotic microorganism of the nursery pig under different temperature and humidity environment state test conditions and the temperature and humidity indexes of the corresponding environment, selects a nasal cavity eukaryotic microorganism single index building model with stronger correlation with the temperature and humidity environment state (selection standard: correlation analysis | r |)>0.6，p<0.05), and also comprehensively combining the relative abundance indexes of the eukaryotic microbes in the nasal cavity and corresponding temperature and humidity indexes, establishing and selecting a model by utilizing Matlab software (selection standard: r2>0.8 requirement p relating to p value<0.05) searching a nasal cavity eukaryotic microorganism relative abundance index combination capable of accurately reflecting the temperature and humidity environment state, evaluating the temperature and humidity environment state of the nursery pig in a period of time by using the nasal cavity eukaryotic microorganism relative abundance index and expressing the temperature and humidity index prediction value, so that the temperature and humidity state evaluation of the growth of the nursery pig is more objective and reasonable.
The animal in the invention is a nursery pig, and can also be popularized to other growing stages of live pigs and other animals and people.
The single index and the index combination of the relative abundance of other eukaryotic microbes in the nasal cavity of the nursery pig, which are not mentioned in the invention, can construct a model through unitary or multiple regression analysis and environmental temperature and humidity index values; in the production stages of other pigs (fattening pigs, growing pigs and the like), a model can be constructed by single indexes and index combinations of eukaryotic microorganisms in nasal cavities of nursery pigs and the environmental temperature and humidity index values through unitary or multiple regression analysis and is used for evaluating the environmental temperature and humidity state; are also within the scope of the present invention.
Compared with the prior art, the invention has the beneficial effects that:
the invention can accurately evaluate the temperature and humidity environment (expressed by temperature and humidity index values) of the nursery pig in the past short time by measuring the relative abundance of the eukaryon microbes in the nasal cavity corresponding to the nursery pig and utilizing the model. The calculated temperature and humidity index of the environment where the nursery pig is located has guiding significance for a manager to adjust factors such as temperature and humidity of the nursery pig house, and the temperature and humidity environment for growth of the nursery pig can be controlled to be always in the optimal state in the production process by combining with the standard of the growth environment of the nursery pig.
Drawings
Fig. 1 shows the temperature-humidity index change trend during the test: the abscissa is time, the ordinate is a temperature and humidity index value, the test is started from 12:00 on the first day to 6:00 on the next day, the observation time interval is uniform and is 30min (the temperature and humidity environment of the breathing chamber in the observation time period is in a stable state), the temperature and humidity index value in the graph is calculated by a formula THI of 0.81Td + (0.99Td-14.3) RH +46.3 of the dry bulb temperature and the relative humidity measured by a temperature sensor and a humidity sensor of the breathing chamber, and the temperature and humidity index value in the small frame in the graph is an average value in the total process of the test (the temperature and humidity index value observed in the test time period is obtained by averaging).
Detailed Description
The experimental methods, materials and reagents used in the examples described below are all conventional methods, materials and reagents, and are commercially available, unless otherwise specified.
1. Test animal
40 large white-long white binary hybrid nursery pigs with no significant difference in body weight were selected and randomly divided into 5 groups of 8 (n is 8) each, and each experimental nursery pig in each group was raised in a breathing chamber in a single pen, and sufficient drinking water was supplied to provide the same formulated feed according to the NRC standard for free feeding.
2. Test procedure and sample Collection
The temperature and humidity environment of the nursery pigs in the breathing heat measuring cabin is controlled, the temperature and humidity environment of each group is the same, and the temperature and humidity environment in the experiment is controlled by an air conditioner. After later calculation, the environmental temperature-humidity indexes of the growth of the 5 groups of test pigs in the test period are 57.5, 62.1, 74.4, 81.8 and 83.7 respectively (the calculation process is described in the attached drawing). Each test pig is raised in a stable warm and humid environment for about 20 hours, and then the nasal vestibule of the nursery pig is scraped by medical sterilized cotton to collect eukaryotic microorganism samples of the nasal cavity of the nursery pig.
3. Nasal cavity microbial sample detection and data analysis
18S rDNA sequencing using the IonS5TMXL sequencing platform was used to detect the relative abundance of various eukaryotic levels in nasal microbial samples. Utilizing IBM SPSS staticisics software to make correlation analysis of relative abundance of single nasal cavity eukaryotic microorganism and test warm-wet index, and selecting nasal cavity eukaryotic microorganism single index with strong correlation with warm-wet environment state to make establishment model (selection standard: correlation analysis | r |)>0.6，p<0.05), then comprehensively combining the relative abundance indexes of the eukaryotic microbes in the nasal cavity and corresponding temperature and humidity indexes, establishing and selecting a model by utilizing Matlab software (selection standard: r2>0.8 requirement p relating to p value<0.05) and finding a blood metabolite index combination capable of accurately reflecting the temperature and humidity environment state.
4. Test results (see Table 1 and FIG. 1)
Table 1 shows that the nasal cavity eukaryotic microorganism indexes of the nursery pigs are used for calculating the temperature-humidity index regression equation of the environment where the nursery pigs are located
The single index of relative abundance of eukaryotic microbes in the nasal cavity cannot establish a regression model meeting the selection standard with the environmental temperature and humidity index, but the eukaryotic microbes in the nasal cavity are utilizedThe index combination is feasible for evaluating the environmental temperature-humidity index of the nursery pig in a short period of time. The best model for evaluating the temperature and humidity state of the environment where the nursery pig grows by utilizing the combination of the eukaryon microorganisms in the nasal cavity is as follows: THI 80.43+509.55x1-112454.19x2-150.30x3-27328.24x4+69.95x5+11819.60x6+292830.3x7+7566.22x8-657.14x9-246075.5x10+32726.74x11-5440.98x12-15061.11x13+16105.21x14-45.89x15+110.18x16-42.21x17+26.51x18-5.98x19-31.19x20-1325.87x21+782.44x22+108.99x23-40055.25x24-132290.1x25+1083.27x26+11120.24x27-44335.50x28+2783.59x29-29868.41x30-3012.42x31-137311.8x32+1149.58x33-31.01x34-3115.54x35-6.96x36-49597.7x37-3632.9x38-377.93x39-232.58x40-12.80x41+39827.06x42-1074.11x43+1585.58x44+30.82x45-1343.39x46-91516.32x47-198.74x48+59262.55x49-9029.64x50-197163.71x51(phylum, class, order, family, genus, species level synthesis, LASSO model, determining coefficient R20.9925), wherein THI is the temperature and humidity index of the environment where the nursery pig is located, x1To x51The nursery pig nasal eukaryotic microorganisms Arthropoda phylum, Euglenida (Euglenida), Chromorpha (Chromadorea), Coccomyxa (Trebouxiophyceae), Trichoronematales (Ochromonales), Brassicales, Fagaleles, Cyclophytales (Sphaeroples), Rhizophyllidae, Eurotiales (Oygenales), Anthroathecacates, Aphaniales (Ulotrichalles), Cystofiloides, Lipotheceae, Rhizophyllophyceae, unclassified Cyclophytales (unidentified _ Sphaeroples), Zea, Tetrahymena (Tetrahymena), Oryza, Trichosporon (Trichosporon), Chloromyces (Trichosporon), Sarcophythora (Trichosporon), Trichosporon (Trichosporon) and the genus,Hordeum, Arabis, Coccomyxa (Trebouxia), Malus, Epinephelus (Colpoda), Naganishia, Candida, Aegium, Sterkiella, Hemiptelea, Spermartissinesia, Rhizomucor (Rhizomucor), Chlamydonella, Pedosphaera, Rhizochythora (Rhizophyllum), Synechocystis (Rhizophyllum), Synthetaiotaomicron (Distima), Tausonia, Rhabdications _ humicola, Trichosporon _ corefaciens, Zea _ mays.1, Blastocystis _ subtype _3, Tremella _ Tremella, Ambrophyceae:, Micrococcus, Micro.
Claims (3)
1. A method for evaluating the warm and humid environment state of a nursery pig individual in a growing environment based on the relative abundance of eukaryotic microbes in a nasal cavity is characterized in that the relative abundance of eukaryotic microbes in the phylum, class, order, family, genus and species level of the nasal cavity of the nursery pig relative to total eukaryotic microbes in the nasal cavity is quantitatively detected, and the relative abundance is combined to evaluate the warm and humid environment state of the nursery pig individual in growing, wherein the eukaryotic microbes comprise: arthropoda phylum; liliopsida, Saccharomyces (Saccharomyces), oligohymenomycetes (Oligohymenophora), Chrysophyceae (Chrysophyceae), Chromorpha (Chromadorea), Chaetomium (Sordariomycetes), Tremellaceae (Tremellomycetes), Pezizomycetes (Pezizomycetes), Arachnida, Coccomyycetes (Trebouxiophyceae), Mononidae (monolonta), Chlorophycetes (Chlorophyceae), Malassiomycetes, Cryptophyceae (Cryptophyceae), Trichosporoides (Spirotria), Hydrozoa (Hyzoea); poales, Saccharomyces (Saccharomyces), Dimethylgentida (Diplophores), Hypocreales (Hypocrea), Trichosporonales, Neocallimastigales, Theales (Lecanorales), Eurotiales (Eurotiales), Brassicales, Rosales, Chlamydomonadales, Malassezia (Malasseziales), Solanales, Filobasidiales (Filobasidiales), Stolidocarchiales, Fagaleles, Cyclophytales (Sphaerpales), watchcase (Arcelllinida), Tremellales (Tremellales), Fucales (Ulotriles), Pyramodiales, Hymenochaetales (Hypocreales), Hypocreales (Ochrotides); saccharomycetaceae (Saccharomyces cerevisiae), Trichosporon aceae, Erythroxylaceae (Nectriaceae), Malasseziaceae, Phaffoylaceae (Hymenochaetaceae), Mrakiaceae, Lichtheimaceae, Rhizophyllaceae; zea, Zymomonas (Kazachstania), Trichosporon (Ochromonas), Scorzonera (Sarocladium), Bromeliothrix, Debaryomyces (Debaryomyces), Malassezia (Malassezia), Malus, Rhizopus (Rhizopus), Naganishia, Candida, Sterkiella, Hemiptelelea, Spermartissella, Rhizomucor (Rhizomucor), Chlamydonella, Phytophthora (Distigma), Tausonia, Hydnochaete (Hydnochaete), Tetrahymena (Tetrahymena), Oryza, Trichosporon (Trichosporon), Homusum, Arposis (Trepophyella), Phyllospora, Rhizoctonia (Rhizoctonia), and Rhizoctonia (Rhizoctonia); zea _ mays species, Kazachstania _ telluris species, Tetrahymena _ corrisis species, Rhabditides _ humicola species, Blastocystis _ sp.subtype _3 species, Candida albicans (Candida _ albicans), Trebouxia _ jamesi species, Naganishia _ vischni species, Diutina _ rugosa species, Hemipherea _ davidi species, Spermanitiella _ europaea species, Bahusaka _ longispora species, Distingma _ proteus species, Taussonia _ pulullans species, Hydnochaete _ dui, Silk (Trinospora _ corefaciens), Aspergillus _ pullulan _ 1 species, Amenstalaria _ pullulans species, or Laminaria _ strain.
2. The method according to claim 1, characterized in that the method comprises the following specific steps:
(1) establishing and selecting a regression model of the relative abundance of the nasal microorganisms and the environmental temperature-humidity index of the nursery pig reflecting the temperature-humidity environmental state of the growing nursery pig as follows:
① class level, THI 77.04-12.85x1-82.96x2+38.29x3+93.41x4-8.59x5-562.97x6-853.91x7+772.03x8+1543.54x9-24754.73x10+1117.40x11-4959.84x12+6713.67x13+212386.71x14-3805.85x15+30401.80x16(ii) a The model is LASSO model, and coefficient R is determined2＝0.8613；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x16The relative abundance of the eukaryotic microorganisms Liliopsida, Saccharomyces, Oligochaeta, Chrysophyceae, Chromophyceae, Chaetomium, Tremellaceae, Panomycetes, Arachnida, Coccomyyceae, Mononidae, Chlorophyceae, Malasseziomyces, Cryptophyceae, Demospongiae, Hydrozoa relative to the total eukaryotic microorganisms of the nasal cavity is respectively as follows: percent;
② mesh level, THI 76.43-17.68x1-125.78x2-245.52x3-669.06x4-1091.73x5+1195.59x6-4130.28x7+3832.18x8+5401.91x9-22665.39x10-1262.74x11+6273.57x12-1213.96x13+94177.67x14-8138.89x15+778026.7x16+4940.84x17+95728.04x18-20800.44x19-104152.25x20+72337.23x21-260723.7x22+1.47x23-44706.57x24(ii) a The model is LASSO model, and coefficient R is determined2＝0.8613；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x24The relative abundance of the nursery pig nasal eukaryotic microorganisms, Poales, Saccharomycoporiales, Digastriales, Hypocrea, Trichosporonales, Neocallimastigales, Theales, Eurotiales, Brassicales, Rosales, Chlamydomonadales, Malaytales, Solanales, Nepalustaria, Stolidobrachia, Fagaales, Cyclophytales, watchcase, Tremellales, Aphyllophorales, Pyramiodales, Dermatophyllales, Filiperidiales, Cystoobasidiales, relative to the total eukaryotic nasal microorganisms, in units of: percent;
③ family level of THI 74.69403-118.91x1-114.10x2-293.41x3+577.06x4-718.67x5-130563.55x6-8165.30x7(ii) a The model is LASSO model, and coefficient R is determined2＝0.8301；
Wherein THI is the temperature-humidity index of the environment of the nursery pig, x1To x7Relative abundance of the nursery pig nasal cavity eukaryotic microorganisms saccharomycetaceae, trichosporinaceae, erythrocasaceae, Malasseziaceae, phaffomomycetaceae, erythrochaceae, Mrakiaceae relative to nasal cavity total eukaryotic microorganisms in units of: percent;
④ genus level, THI 78.76-30.35x1-36.64x2+16.03x3-1109.55x4-2820.99x5-206.42x6+354.05x7-44705.11x8-22761.51x9+21032.98x10-35505.62x11-33058.75x12-27239.03x13-13604.5x14+165745.7x15-1.78x16-93578.76x17-41798.36x18-190048.3x19(ii) a The model is LASSO model, and coefficient R is determined2＝0.9861；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x19Respectively belong to the genus Zea, the genus Zymomonas (Kazachstania) and the genus Zoflagellate which are eukaryotic microorganisms of nasal cavity of nursery pig(Ochromonas), Scorzonera (Sarocladium), Bromeliothrix, Debaryomyces (Debaryomyces), Malassezia (Malassezia), Malus, Rhizopus (Rhizopus), Naganishia, Candida, Sterkiella, Hemiptelea, Spermartissella, Rhizomucor (Rhizomucor), Chlamydonella, Phytophthora (Distima), Tausonia, Hymenochaetes (Hydnochaete) relative to the relative abundance of total eukaryotic microbes in the nasal cavity in units of: percent;
⑤ levels of THI 77.30-26.08x1-101.62x2+12.81x3-393.02x4+5037.59x5-2847.75x6-18478.26x7+42621.47x8-31045.96x9-49866.91x10-49842.29x11+7253.92x12-80561.46x13-17228.1x14-59962.73x15(ii) a The model is LASSO model, and coefficient R is determined2＝0.9741；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x15The relative abundance of the eukaryotic microorganisms Zea _ mays, Kazachstania _ telluris, tehymena _ corrisis, rhabdidodes _ humicola, blastystitis _ sp, subtype _3, candida albicans, Trebouxia _ jamesi, naganisia _ vismanii, Diutina _ rugosa, hemipteea _ davidi, spenrmartheiella _ europaa, bausakala _ longispora, Distigma _ proteus, Tausonia _ pullulans, hydnocharea _ duii relative to the total eukaryotic microorganisms in units of: percent;
⑥ Total THI 80.43+509.55x1-112454.19x2-150.30x3-27328.24x4+69.95x5+11819.60x6+292830.3x7+7566.22x8-657.14x9-246075.5x10+32726.74x11-5440.98x12-15061.11x13+16105.21x14-45.89x15+110.18x16-42.21x17+26.51x18-5.98x19-31.19x20-1325.87x21+782.44x22+108.99x23-40055.25x24-132290.1x25+1083.27x26+11120.24x27-44335.50x28+2783.59x29-29868.41x30-3012.42x31-137311.8x32+1149.58x33-31.01x34-3115.54x35-6.96x36-49597.7x37-3632.9x38-377.93x39-232.58x40-12.80x41+39827.06x42-1074.11x43+1585.58x44+30.82x45-1343.39x46-91516.32x47-198.74x48+59262.55x49-9029.64x50-197163.71x51(ii) a The model is LASSO model, and coefficient R is determined2＝0.9925；
Wherein THI is temperature and humidity index of environment where nursery pig is located, x1To x51The species are the eukaryotic microorganisms Arthropoda phylum, Euglena, Chromomycete, Coccomyxa, Zosterales, Brassicales, Fagaleles, Cyclocystiles, Rhizophylldactyles, Eurycolatoles, Anthioatheca, Aphyllophorales, Cystofilobiales, Lichtheimaceae, Rhizophyllaceae, unclassiformes, Zea, Tetrahymena, Oryza, Trichosporoides, Scopulariopsis, Hordeum, Arabis, Coccomyeria, Malus, nephrospora, Naganishia, Candida, Auciliopsis, Sterkia, Hemiptelegilia, Spermamyces, Blastomyces, Tremella, Schizosaccharomyces, Thermoascus, Schizosaccharomyces, Polyporus, Schizosaccharomyces, Thermoascus Ammopiptanthus mongolicus strain relative to total eukaryotic microorganisms of nasal cavityThe relative abundance of an object in units of: percent;
(2) and quantitatively measuring the relative abundance of eukaryotic microorganisms in the nasal cavity of the nursery pig, substituting the measured result into a corresponding regression model, and calculating to obtain the temperature and humidity index of the environment in which the nursery pig is located in a short period.
3. The method of claim 2, wherein the nasal cavity eukaryotic microorganism relative abundance indicator is obtained based on 18S rDNA sequencing of the ion S5TMXL sequencing platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010158494.1A CN111681707B (en) | 2020-03-09 | 2020-03-09 | Method for evaluating temperature and humidity state of growth environment of individual nursery pigs based on relative abundance of nasal eukaryotic microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010158494.1A CN111681707B (en) | 2020-03-09 | 2020-03-09 | Method for evaluating temperature and humidity state of growth environment of individual nursery pigs based on relative abundance of nasal eukaryotic microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111681707A true CN111681707A (en) | 2020-09-18 |
| CN111681707B CN111681707B (en) | 2023-09-05 |
Family
ID=72433234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010158494.1A Active CN111681707B (en) | 2020-03-09 | 2020-03-09 | Method for evaluating temperature and humidity state of growth environment of individual nursery pigs based on relative abundance of nasal eukaryotic microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111681707B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104962620A (en) * | 2015-06-10 | 2015-10-07 | 宁波大学 | Microflora-based ecology health evaluation method |
| WO2016172686A1 (en) * | 2015-04-24 | 2016-10-27 | The Translational Genomics Research Institute | Compositions and methods for augmenting the nasal microbiome |
| CN107849597A (en) * | 2015-06-25 | 2018-03-27 | 埃斯库斯生物科技股份公司 | For analyze the microbial strains from complex heterogeneous group, prediction and identify its functional relationship and interaction and based on its selection and synthesized micro-organism group method, apparatus and system |
| WO2018067887A1 (en) * | 2016-10-05 | 2018-04-12 | Virginia Commonwealth University | Bacterial profile to detect fungal taxa abundance in the gut |
| CN109407624A (en) * | 2018-07-19 | 2019-03-01 | 天津农学院 | A kind of cowshed environment conditioning system and its regulation method based on humidity-temperature index |
| WO2019051130A1 (en) * | 2017-09-06 | 2019-03-14 | uBiome, Inc. | Nasal-related characterization associated with the nose microbiome |
| WO2019191649A1 (en) * | 2018-03-29 | 2019-10-03 | Freenome Holdings, Inc. | Methods and systems for analyzing microbiota |
| CN110419499A (en) * | 2019-08-28 | 2019-11-08 | 广西壮族自治区水牛研究所 | A method of evaluation lactation period dairy buffalo comfort |
-
2020
- 2020-03-09 CN CN202010158494.1A patent/CN111681707B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016172686A1 (en) * | 2015-04-24 | 2016-10-27 | The Translational Genomics Research Institute | Compositions and methods for augmenting the nasal microbiome |
| CN104962620A (en) * | 2015-06-10 | 2015-10-07 | 宁波大学 | Microflora-based ecology health evaluation method |
| CN107849597A (en) * | 2015-06-25 | 2018-03-27 | 埃斯库斯生物科技股份公司 | For analyze the microbial strains from complex heterogeneous group, prediction and identify its functional relationship and interaction and based on its selection and synthesized micro-organism group method, apparatus and system |
| WO2018067887A1 (en) * | 2016-10-05 | 2018-04-12 | Virginia Commonwealth University | Bacterial profile to detect fungal taxa abundance in the gut |
| WO2019051130A1 (en) * | 2017-09-06 | 2019-03-14 | uBiome, Inc. | Nasal-related characterization associated with the nose microbiome |
| WO2019191649A1 (en) * | 2018-03-29 | 2019-10-03 | Freenome Holdings, Inc. | Methods and systems for analyzing microbiota |
| CN109407624A (en) * | 2018-07-19 | 2019-03-01 | 天津农学院 | A kind of cowshed environment conditioning system and its regulation method based on humidity-temperature index |
| CN110419499A (en) * | 2019-08-28 | 2019-11-08 | 广西壮族自治区水牛研究所 | A method of evaluation lactation period dairy buffalo comfort |
Non-Patent Citations (4)
| Title |
|---|
| RUIXIA LAN等: "Effects of probiotic supplementation in different nutrient density diets on growth performance, nutrient digestibility, blood profiles, fecalmicroflora and noxious gas emission in weaning pig", 《WILEY ONLINE LIBRARY》, pages 1335 * |
| 杨飞云等: "畜禽养殖环境调控与智能养殖装备技术研究进展", 《中国科学院院刊》, pages 163 - 173 * |
| 鞠雷等: "保育猪舍不同粒径悬浮颗粒物细菌 群落组成的初步研究", 《畜牧兽医学报》, vol. 28, no. 11, pages 2198 - 2204 * |
| 韩一超等: "保育猪舍空气、粪便、鼻腔黏液及猪组织器官的微生物群落多样性分析", 《畜牧与兽医》, vol. 50, no. 10, pages 32 - 37 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111681707B (en) | 2023-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103013761B (en) | Full-automatic distiller's yeast preparation machine | |
| CN103173365B (en) | Method for indoor culture breeding of Plasmopara viticola | |
| CN111681707A (en) | Method for evaluating temperature and humidity state of growing environment of nursery pig individual based on nasal cavity eukaryotic microorganism relative abundance | |
| CN106168818B (en) | A kind of cultivating edible monitoring system and its method | |
| CN101138385B (en) | Apparatus and method for producing feedstuff by cattle manure | |
| CN111621577A (en) | Method for evaluating temperature and humidity state of growing environment of nursery pig individual based on nasal cavity prokaryotic microorganism relative abundance | |
| CN201153422Y (en) | Intelligent monitoring apparatus for hothouse | |
| CN104543402B (en) | A kind of preparation method for the state iron feed additive that ferments | |
| CN205774557U (en) | A kind of intelligent constant-temperature culture apparatus | |
| CN105830817B (en) | A kind of auto temperature control system for shed | |
| CN111676301B (en) | Method for evaluating temperature and humidity state of environment where nursery pigs are located based on relative abundance of nasal microorganisms | |
| CN207022429U (en) | A kind of feeding millet fast germination device | |
| CN108658635A (en) | A kind of organic fertilizer aerobic fermentation control system | |
| CN206219584U (en) | A kind of suspension cell lesion terminal automatic identification and control system | |
| CN113678688A (en) | Flammulina velutipes culture method based on liquid strain culture | |
| CN106094945B (en) | A kind of cultivating edible monitoring system and its method | |
| CN107114119B (en) | A method of Cordyceps militaris is cultivated using thermal stimulation | |
| CN209749353U (en) | Integrated intelligent bacterial cultivation room of thing networking | |
| CN109632564A (en) | The measuring method of grain water content after a kind of wheat physiological maturity | |
| CN203007249U (en) | Fully-automatic distiller's yeast preparation machine | |
| CN104408314A (en) | Prediction method for lead content fading in lactation of dairy cow and application | |
| CN204369874U (en) | A kind of clinical medicine medical treatment viable bacteria incubator | |
| CN213073789U (en) | Cultivation box for Japanese thin-skin walnut seedlings | |
| CN104911054B (en) | A kind of liquid simulation cultivation of traditional Daqu and products thereof | |
| CN206081112U (en) | Fermentation equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |