CN107114119B - A method of Cordyceps militaris is cultivated using thermal stimulation - Google Patents
A method of Cordyceps militaris is cultivated using thermal stimulation Download PDFInfo
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Abstract
The present invention relates to a kind of methods for cultivating Cordyceps militaris using thermal stimulation.This method is implementing day and night temperature stimulation with the next stage to Cordyceps militaris：Stage 1：The stage that Cordyceps militaris former base forms fructification young shoot is grown to since Cordyceps militaris former base；Stage 2：The stage of spore head is initially formed from fruiting bodies of cordyceps militaris height up to 1.5 2cm to fructification；The thermal stimulation is to make the temperature on daytime 56 DEG C higher than the temperature in evening in the range of not departing from Cordyceps militaris growth temperature.The method of the present invention can significantly improve the former base quantity of Cordyceps militaris and improve the size of spore head at the top of fructification, to improve single box net weight and value of the product.
Description
Technical field
The present invention relates to the technical fields of Applied Biotechnology principle artificial culture fungi, and in particular to the training of Cordyceps militaris
It educates, relates more specifically to a kind of method that Cordyceps militaris is cultivated using thermal stimulation.
Background technology
Cordyceps militaris, that is, cordyceps flower, also known as northern Chinese caterpillar Fungus are Ascomycota, and cordyceps sinensis Cordycepps, Cordyceps militaris color is orange
Or Chinese red, overall length 3-8cm, it belongs to Eumycota Cordyceps with Cordyceps of Qinghai Province, but is different kinds.Pharmacological action is modern
Scientific appraisal, Cordyceps militaris not only have special nutritive value, but also have apparent medical value, have cordycepin, cordycepic acid,
Several big cordyceps sinensis ingredients such as Cordyceps sinensis polysaccharide have with the almost the same drug effect of cordyceps sinensis.Cordyceps militaris is more domestic as Cordyceps
Outer scholar generally receives.Cordyceps militaris history of being used as medicine is not long, but Cordyceps militaris is biologically belonging to a category with cordyceps sinensis,
Have a very same or analogous chemical composition, and the cordycepin of Cordyceps militaris, K, Ca, Se, vitamin etc. it is beneficial to human body at
The content divided is higher than wild cordyceps, but the Cordyceps militaris price cultivateeed, but well below wild cordyceps, this is just
Good market prospects are brought to the production of Cordyceps militaris.Moreover, Cordyceps militaris has obtained national Chinese medicine one kind medicinal material examination ＆ approval production.With
Approval of the people to Cordyceps militaris, the figure of Cordyceps militaris is gradually appeared on the dining table of common people, this promotes the market of Cordyceps militaris
Demand also constantly expands.The cultivation of Cordyceps militaris produces, and it is extensive can to carry out factory as technologically advanced " smokeless industry "
Production.
Existing Cordyceps militaris is in early growth period（Cordyceps militaris former base separates before fructification young shoot）, control temperature is in 18-
20 DEG C, humidity is in 60%-70%, growth medium（Former base be differentiated to form fructification and gradually when growing up）Temperature is controlled in 20-25
DEG C, humidity is in 80%-95%, and Later growth is i.e. close to the stage of ripeness（The close maturation of fructification）Temperature is controlled at 18-20 DEG C, humidity
In 60%-70%.In the breeding method of existing Cordyceps militaris, it is only necessary to which ensuring that temperature and humidity is in defined numberical range can tie up
Hold growth, i.e. the day and night temperature difference is little, most Cordyceps militaris can normal growth, but the Cordyceps militaris cultivated
It cannot meet the market demand in density and spore head size.
Invention content
The present invention provides a kind of method for cultivating Cordyceps militaris using thermal stimulation, implements round the clock to Cordyceps militaris with the next stage
Thermal stimulation：
Stage 1：The stage that Cordyceps militaris former base forms fructification young shoot is grown to since Cordyceps militaris former base；
Stage 2：Spore head is initially formed from fruiting bodies of cordyceps militaris height up to 1.5-2cm to fructification（Namely top end diameter
It is rounded more than strain body diameter or top）Stage；
The thermal stimulation is to make the temperature on daytime than the temperature in evening in the range of not departing from Cordyceps militaris growth temperature
It is 5-6 DEG C high（I.e. daytime has 5-6 DEG C of difference with night in temperature）.
Further, the stage 1 is after Cordyceps militaris aerial hyphae annesl completes and sweep out aerial hyphae, and Cordyceps militaris starts
Grow the stage that former base forms fructification young shoot to Cordyceps militaris former base.
Further, aerial hyphae is swept out with old mycelia remover tool, and aerial hyphae is made to lodge on culture medium in the same direction.
Further, 1 duration of the stage is 7-10 days.
Further, the stage 2 is that fruiting bodies of cordyceps militaris height reaches 1.5-2cm, and long white point is real to son at the top of fructification
Start to expand and occur the stage of powder at the top of body.
Further, for the stage 2, when fruiting bodies of cordyceps militaris young shoot height reaches 1.5-2cm, and the fructification of long white point
When reaching 50% or more, start thermal stimulation.
Further, in the cultivation in Cordyceps militaris stage 1 and stage 2, it is 18-25 DEG C to make the temperature on daytime, preferably 23 DEG C, late
On temperature be 15-20 DEG C, preferably 18 DEG C, and make day and night temperature be 5-6 DEG C.
Further, the time point that day alternates with night is 7:30 and 17:30.
Further, in the cultivation in Cordyceps militaris stage 1 and stage 2, light application time 15-19 hours, intensity of illumination 100-150
Lux.
This method is easy to operate, can be highly practical, and there is generality and versatility, key to be to grasp the two temperature difference
The critical stage of stimulation.Wherein, day temperature is higher, is conducive to photosynthesis of plant, generates a large amount of organic matter, night temperatures
After reduction, the respiration of Cordyceps militaris reduces, and reduces the consumption of organic matter, so plant can effectively be improved by improving day and night temperature
The photosynthesis of object promotes the storage of plant idiotrophic substance, can carry out better photosynthesis on daytime.But if
Thermal stimulation not being carried out to Cordyceps militaris, the night organic matter of consumption is also continuing, and subsequent growth can be influenced accordingly,
And then the former base of fruiting bodies of cordyceps militaris, single box net weight is caused to be affected.
The method of the present invention can significantly improve the former base quantity of Cordyceps militaris and improve the size of spore head at the top of fructification, from
And improve single box net weight and value of the product.Specifically：Effect one can increase Cordyceps militaris and go out the quantity of former base, and improve later stage pupa
Cordyceps sinensis former base is differentiated to form the density of fructification, increases single box yield.Effect two can increase the straight of spore head Cordyceps militaris spore head
Diameter increases the rugosity of rodlike pupa worm strain body.The diameter of Cordyceps militaris spore head determines the quantity of conidia powder, and then influences pupa worm
The medical value and economic value of grass.For single strain body, thick Cordyceps militaris will be better than on nutritive value and weight
Thin Cordyceps militaris, therefore the thermal stimulation in the two stages is for increasing the density of fruiting bodies of cordyceps militaris, rugosity increases list in turn
Box net weight and yield include that the sale in later stage is all of great advantage.
Specific implementation mode
The thermal stimulation that the present invention carries out was started from respectively with the next stage：Stage 1：Cordyceps militaris spawn is cultivated in compost
Sweep to the snow-white aerial hyphae of generation and annesl and by aerial hyphae the growth phase opened and generate fruit body primordium successively；Rank
Section 2：Fructification grows to 1.5-2cm, when the visible fine white pointing object in top.
In stage 1, it is stained with sterile water using wooden hair planting brush and made of metal stainless steel wire scratches method and sweeps out aerial hyphae.Rank
Stimulation the invention of section 1 carries out in the stage of growth former base.Cordyceps militaris is in former base growth course by rational between the morning and evening
Thermal stimulation can stimulate the generation of more former bases, wherein in the normal temperature range that Cordyceps militaris is cultivated, make day temperature phase
To higher, the temperature of media surface similarly increases, and so that temperature is declined to night.Continue full heat and energy daytime, and
Evening temperature is low to can be very good storage heat and energy, and more former bases can be stimulated to generate.
In stage 2, thermal stimulation is carried out during fruiting bodies of cordyceps militaris shoot growth, it can repetitious stimulation Cordyceps militaris son reality
Nutriment is not stopped to be sent to top, causes fruiting bodies of cordyceps militaris gradually to expand and become larger by body top area, fruiting bodies of cordyceps militaris
Cordyceps militaris spore head is formed, spore powder content is more.Without the fruiting bodies of cordyceps militaris top by thermal stimulation due to its own
Strain classification reason can also expand, but its spore head unobvious, not full or not mellow and full for being formed, and spore powder content is few.
Two key points of the present invention are：1）Night on daytime thermal stimulation, but it cannot be detached from the growth temperature of Cordyceps militaris,
The too high or too low growth that may stop former base and the variation of later stage Cordyceps militaris, stop growing, contamination rate increase etc..2）Temperature
The purpose of difference stimulation is to improve the density of Cordyceps militaris, yield to meet the market requirement, therefore two stages must be grasped.
This two stages must be diligent in observing, and otherwise can run counter to desire instead falls flat.
Thermal stimulation starts from a more specific embodiment,：Stage 1, in Cordyceps militaris wooden hair planting brush and made of metal
Stainless steel wire is scratched out after culture medium aerial hyphae 3-5 days, and son is initially formed on the visible aerial hyphae in Chinese caterpillar fungus culture medium surface
When entity former base, start thermal stimulation；Stage 2, when fruiting bodies of cordyceps militaris height has grown to 1.5-2cm, and fructification top
There is small particles in portion, and more preferably when white point accounts for 50% or more of whole box, and entirely lot number has 50% or more and such case class
Like when, start thermal stimulation.
The Cordyceps militaris spawn can be commercially available various Cordyceps militaris spawns, the choosing of method of the invention to Cordyceps militaris spawn
It selects and does not specially require, can improve the density of fruiting bodies of cordyceps militaris and thick through the invention not being the strain of spore head
Degree.Wherein, the Cordyceps militaris spawn can be before being inoculated in culture medium, and according to the conventional method of this field, culture in advance is
The form of solid spawn or liquid spawn, the preferably form of liquid spawn.Wherein, the Cordyceps militaris spawn can be according to routine
Method be inoculated in culture medium, the present invention does not require particularly.
In the stage 1 of the present invention, it is desirable that illumination reaches 15-19 hours, intensity of illumination 100-150 Luxs, day temperature
18-25 DEG C, with 23 DEG C for optimum temperature, but no more than 25 DEG C or more, evening temperature is 15-20 DEG C, be with 18 DEG C it is best,
But cannot be below 15 DEG C, otherwise the fruiting bodies of cordyceps militaris long-time low temperature stimulation later stage can influence fruiting bodies of cordyceps militaris bud ratio and son
Physical height.In the stage 2 of the present invention, it is desirable that illumination reaches 15-19 hours, intensity of illumination 100-150 Luxs, day temperature
18-25 DEG C, with 23 DEG C for optimum temperature, but no more than 25 DEG C or more, evening temperature is 15-20 DEG C, be with 18 DEG C it is best,
But 15 DEG C are cannot be below, temperature is excessively high, can promote the growth of miscellaneous bacteria and cause fruiting bodies of cordyceps militaris later stage lighter, in advance
Aging, and temperature is too low, and Cordyceps militaris required temperature is not achieved, fructification will stop growing, and cause later stage fruiting bodies of cordyceps militaris strain
Body too short influences condition.
The data such as temperature, humidity, illumination in the present invention be by multiple example field conduct, by verifying repeatedly to
The method of obtained best, most suitable thermal stimulation cultivation cordyceps sinensis.
In one embodiment, for the stage 1, Chinese caterpillar fungus culture medium long former base stage daily light application time is 15-
19 hours, intensity of illumination was 150 Luxs, relative air humidity 70%, morning 7 on daytime：30- evenings 17：30, temperature is kept
At 23 DEG C, temperature it is not up to standard it is high or low can by adjust floor heating, adjust air-conditioning, the modes such as the wind that begins to rehearse promote temperature up to standard.
At night 17：30- the next mornings 7：30, temperature is maintained at 16-18 DEG C.For Cordyceps militaris in incubation, especially night stops light
It can be suitably by night when night culture room light is closed according to the best period for being Cordyceps militaris suspend mode storage energy
Temperature is turned up 1-2 DEG C, does not radiate to influence to cultivate room bulk temperature to make up closing light.For the stage 2, Cordyceps militaris is real
Culture room humidity is maintained at 80% when body grows 1.5-2cm, and light application time 15-19 hours, intensity of illumination is 100-150 Luxs.
In one embodiment, for the stage 1, the culture room of thermal stimulation is carried out, morning 7 on daytime：30- evenings 17：
30, temperature is maintained at 23-24 DEG C, temperature it is not up to standard it is high or low can be by adjusting floor heating, adjusting air-conditioning, the modes such as the wind that begins to rehearse
Promote temperature up to standard.At night 17：30- the next mornings 7：30, temperature is maintained at 15-17 DEG C.Cordyceps militaris is in incubation, especially
It is to stop the best period that illumination is Cordyceps militaris suspend mode storage energy night, when night culture room light is closed,
Can night temperatures be suitably turned up 1-2 DEG C, not radiate to influence to cultivate room bulk temperature to make up closing light, this process needs
Continue 7-10 days or result suitably shortens or extends according to the observation.
Cordyceps militaris cultivates the method that room keeps temperature：Winter, floor heating+electricity air-conditioning（According to air-conditioning number, using face is calculated
Product calculates a culture room and needs air-conditioning quantity）, winter temperature is low, in order to ensure that the temperature on culture room daytime and evening can
Reach the temperature of thermal stimulation, electric air-conditioning first can be transferred to ideal temperature to prevent temperature difference excessive or too small, that night
On by air draft or the modes such as floor heating can be closed cool down when the temperature is excessively high.Summer electricity air-conditioning+water air-conditioning cannot meet when water air-conditioning
When cooling demand, electric air-conditioning setting suitable temperature can be started to prevent mutating.
For example, winter can adjust the temperature in Cordyceps militaris culture room in the following manner：Floor heating（Spacing is between floor heating pipe
1 meter is best）+ electricity air-conditioning（According to air-conditioning number, usable floor area is calculated, a culture room is calculated and needs air-conditioning quantity, air-conditioning
Number is 3, and culture room area is 100 square metres, then needs two）, closing floor heating quantity will be spaced apart, and cannot in succession while close
It closes, in order to avoid cause the height of local temperature different.
The present invention is described in further detail by the following examples, but these contents should not be construed as to the present invention
Limitation.The operating method not stated clearly specifically wherein is carried out according to the routine operation of this field, and reagent and strain for using etc. are equal
Commercially available acquisition.
Embodiment 1
By wheat 350g, culture medium is obtained after water 560ml mixing, putting culture medium under 121 DEG C and 0.14Mpa steam goes out
It is spare to obtain culture medium to room temperature for bacterium 90Min postcoolings.
It completes, to the process of culture medium inoculated, then to carry out the training in light culture stage on superpurgative working table assembly line
It supports.The light culture stage need to meet the following conditions：Temperature is 18 DEG C, relative humidity 60%.Conventional light culture cultivation stage is
10-12 days.Wait for that Chinese caterpillar fungus culture medium charge level covers with snow-white fish scale-shaped aerial hyphae, and charge level bottom Cordyceps militaris mycelia has been pricked
Root is completed, so that it may carry out light-exposed annesl processing so that Chinese caterpillar fungus culture medium to be transferred to the culture room equipped with incandescent lamp.
The culture of light stimulation stage is then carried out, at this time in order to verify thermal stimulation as a result, culture medium is transferred to two not
Same culture room.In order to make aerial hyphae annesl be crocus faster, the condition of the light stimulation cultivation stage includes：Often
It light application time is 18 hours, and intensity of illumination is 150 Luxs, and the temperature of illumination period is 23 DEG C；Temperature during no light
Degree is 23 DEG C, relative air humidity 60%.Aerial hyphae and Cordyceps militaris mycelia inside magazine need to be transformed into crocus or golden yellow
Color.Find that Cordyceps militaris aerial hyphae is higher by media surface 0.02mm or so during annesl, and the fish scale-shaped area rided out is
At 0.6-0.9 square millimeters, at this time the illumination annesl stage terminate, artificial bacteria thorn of sweeping can be entered and swash Cordyceps militaris mycelia growth step
Section.
It is scratched with wooden hair planting brush and made of metal stainless steel wire and sweeps out the aerial hyphae, and make aerial hyphae lodging in institute
State media surface.During sweeping bacterium, unidirectional progress, not repeatedly, aerial hyphae lodging direction are consistent.Then, former base is carried out
The cultivation culture in stage.
Here comparison carries out thermal stimulation and not thermal stimulation（Temperature difference is small）Cultivation.
（The stimulation in stage 1 of the invention）It manually sweeps bacterium to sweep out after the aerial hyphae of Cordyceps militaris surface 4 days, Cordyceps militaris former base is successively
It grows, former base grows stage growth condition and is：Daily light application time is 18 hours, and intensity of illumination is 150 Luxs, relatively empty
Air humidity degree is 70%, daytime 7：30-17：30, temperature is maintained at 23 DEG C.Temperature it is not up to standard it is high or low can by adjust floor heating,
Adjusting the modes such as air-conditioning, the wind that begins to rehearse promotes temperature up to standard.At night 17：30- next day 7：30, temperature is maintained at 18 DEG C.It is trained when night
When foster room light is closed, can night temperatures be suitably turned up 1-2 DEG C, not radiate to influence to make up closing light
Cultivate room bulk temperature.Cordyceps militaris is especially Cordyceps militaris suspend mode storage during night stopping illumination in incubation
The best period of energy.
（The prior art）The culture medium in addition culture room is transferred to 4 days after sweeping out aerial hyphae, Cordyceps militaris former base is grown successively
Go out, former base grows stage growth condition and is：Daily light application time is 18 hours, and intensity of illumination is 150 Luxs, relative atmospheric
Humidity is 70%, 21 DEG C of temperature（It only keeps normal Cordyceps militaris growth temperature in day and night）.Temperature is up to standard can not
With control the air-conditioning that warms up.
After thermal stimulation 3 days, the quantity of Cordyceps militaris former base is observed, finds the Chinese caterpillar fungus culture medium surface by thermal stimulation
Cordyceps militaris former base is still grown successively, and Cordyceps militaris former base density obviously becomes larger, without after the Cordyceps militaris former base of thermal stimulation
Continuous growth significant change is less or seldom, and density increases unobvious.
Continue thermal stimulation after 8 days, it is found that Cordyceps militaris former base has been grown to fructification young shoot, stops temperature difference thorn at this time
Swash, keeps the temperature at 21 DEG C, for humid control 70%, illumination 18 hours, intensity of illumination is 150 Luxs, continues to cultivate.This
When it has been observed that by thermal stimulation fruiting bodies of cordyceps militaris young shoot density be more than without thermal stimulation fructification young shoot.
When fruiting bodies of cordyceps militaris young shoot grows to 1.5-2cm, observation at this time finds that fruiting bodies of cordyceps militaris young shoot top is whitened,
Long white point carries out thermal stimulation again at this time.
Two culture room humidity are maintained at 80%, and light application time 18 hours, intensity of illumination is 150 Luxs.
（The stimulation in stage 2 of the invention）Carry out the culture room of thermal stimulation, morning 7 on daytime：30- evenings 17：30, temperature is protected
Hold at 23 DEG C, temperature it is not up to standard it is high or low can by adjust floor heating, adjust air-conditioning, the modes such as the wind that begins to rehearse promote temperature to reach
Mark.At night 17：30- the next mornings 7：30, temperature is maintained at 17 DEG C.For Cordyceps militaris in incubation, especially night stops light
It can be suitably by night when night culture room light is closed according to the best period for being Cordyceps militaris suspend mode storage energy
Temperature is turned up 1-2 DEG C, does not radiate to influence to cultivate room bulk temperature to make up closing light.
（The prior art）Another should not the culture room humidity of thermal stimulation be maintained at 80%, light application time 18 hours, illumination
Intensity is 150 Luxs, and temperature is maintained at 21 DEG C, i.e., in the range of Cordyceps militaris normal growth.
By thermal stimulation and the suspend mode light stimulation in evening during fruiting bodies of cordyceps militaris shoot growth, pierce repeatedly
Swash fruiting bodies of cordyceps militaris top area, fruiting bodies of cordyceps militaris does not stop nutriment to be sent to top, causes fruiting bodies of cordyceps militaris
Gradually expansion becomes larger to form Cordyceps militaris spore head, and spore powder content is more, without the fruiting bodies of cordyceps militaris top by thermal stimulation
End can also be expanded due to its own strain classification, but the spore head unobvious of its formation, not full or not mellow and full, spore
Sub- powder content is few.
After 7 days, find to start to expand at the top of the fruiting bodies of cordyceps militaris by thermal stimulation, and start powder occur, i.e.,
Restore fructification normal growth stage, daily light application time is 18 hours, and intensity of illumination is 150 Luxs, illumination period
Temperature is 22 DEG C, and the temperature during no light is 20 DEG C, relative air humidity 85%.After 23 days sporophore growth stages, it is seen that
The size of the fructification of Cordyceps militaris is not being grown up substantially, and spinule occurs in top, and Cordyceps militaris nutritive value is relatively high at this time,
It is immediately finished the culture in fructification stage, is harvested.
The present embodiment is total to continue 50-55 days, by a diameter of of the Cordyceps militaris fresh grass spore head after thermal stimulation
0.7-1.2cm does not pass through a diameter of 0.4-0.8cm of the fresh grass spore head of thermal stimulation.
In the present embodiment, technical solution according to the invention, fruiting bodies of cordyceps militaris is undergone altogether in growth course to be pierced twice
Swash, it is therefore an objective to stimulate more former bases to grow, stimulation spore capitiform is at and passing through the fruiting bodies of cordyceps militaris density of thermal stimulation twice
Greatly, spore head is big full mellow and full, and conidia powder is fine and smooth, more, and single box net weight reaches every box 36.3g, and dry product spore head diameter exists
0.5-0.8cm.According to the scheme of the prior art, the growth course of Cordyceps militaris maintains always the optimum ring of Cordyceps militaris growth
Border, but fruiting bodies of cordyceps militaris density is significantly lower than the fruiting bodies of cordyceps militaris by thermal stimulation, and single box net weight is 34.2g/ boxes,
Spore head morphology has not been seen in a diameter of 0.2-0.4 cm after the drying of dry product plane head, part.
Above-mentioned data are the average value that inventor counts.
It follows that the present invention realizes：One, more former base growths are stimulated, increases bud ratio, increases the growth of Cordyceps militaris
Density, to improve yield.Two, Cordyceps militaris carries out thermal stimulation in growth phase can greatly stimulate the life of top spore head
Long, because the difference of temperature is by more nutrient deliveries to top, mellow and full full to reach spore head, spore powder is more, respectively
Item nutrition is more, up to standard, meets the market demand, improves the quality and value of Cordyceps militaris.
Embodiment 2
Compared with embodiment 1 is using the technical solution of thermal stimulation, this embodiment is used on the basis of thermal stimulation
The temperature difference value of bigger（More than 5-6 DEG C）.Operating procedure is as follows（The condition that do not enumerate again is identical as embodiment 1）：
Artificial to sweep bacterium and sweep out after the aerial hyphae of Cordyceps militaris surface 4 days, Cordyceps militaris former base is grown successively, former base grow the stage its
Growth conditions is：Daily light application time is 18 hours, and intensity of illumination is 150 Luxs, relative air humidity 70%, morning on daytime
Morning 7：30- evenings 17：30, temperature is maintained at 25 DEG C, temperature it is not up to standard it is high or low can by adjust floor heating, adjust air-conditioning,
The modes such as wind that begin to rehearse promote temperature up to standard.At night 17：30- the next mornings 7：30, temperature is maintained at 16 DEG C.Cordyceps militaris was being cultivated
Cheng Zhong, especially night stop the best period that illumination is Cordyceps militaris suspend mode storage energy.This process continues 8 days, and observation is found
Cordyceps militaris former base quantity also has apparent increase, and Cordyceps militaris former base is divided into fructification young shoot at this time, stops thermal stimulation.
When fruiting bodies of cordyceps militaris young shoot grows to 1.5-2cm, observation at this time finds that fruiting bodies of cordyceps militaris young shoot top is whitened,
Long white point will carry out thermal stimulation again at this time.Humidity is maintained at 80%, and light application time 18 hours, intensity of illumination is 150 Le gram
Department, morning 7 on daytime：30- evenings 17：30, temperature is maintained at 25 DEG C, temperature it is not up to standard it is high or low can by adjust floor heating,
Adjusting the modes such as air-conditioning, the wind that begins to rehearse promotes temperature up to standard.At night 17：30- the next mornings 7：30, temperature is maintained at 16 DEG C.This mistake
Cheng Chixu 7 days, then restore conventional breeding condition.
It has been observed that the fruiting bodies of cordyceps militaris average length after the increase of 2 thermal stimulation difference of embodiment in embodiment 1 than passing through
The fructification average length for crossing thermal stimulation is slightly higher than 1.1-2mm, a diameter of 0.7-1.2cm of spore head of embodiment 2, with implementation
The spore head diameter of Cordyceps militaris in example 1 by thermal stimulation is similar, but single box dry product net weight is 36.3g, and passes through real
The Cordyceps militaris list box net weight for applying thermal stimulation in example 1 is 34.2g.And it was found that the Cordyceps militaris after the increase of thermal stimulation difference
Contamination rate obviously increases, and causes to harvest yield decline.
By repeatedly practise it is that production obtains statistics indicate that, sweep bacterium artificial and sweep out 3-5 after the aerial hyphae of Cordyceps militaris surface
It, Cordyceps militaris former base grows the stage successively and fruiting bodies of cordyceps militaris young shoot top is whitened, the long white point stage, carries out thermal stimulation training
It brings out the Cordyceps militaris come and is all seized of absolute predominance in height, rugosity, spore head size.Thermal stimulation 7-10 days, you can extensive
Multiple normal aquaculture management（There is flexibility according to actual conditions number of days）, 5-6 DEG C of the temperature difference is advisable, and cultivates temperature and do not depart from Cordyceps militaris
Normal cultivation temperature, the temperature range with Cordyceps militaris suitable growth is best.
Claims (8)
1. a kind of method for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that implement round the clock to Cordyceps militaris with the next stage
Thermal stimulation：
Stage 1：The stage that Cordyceps militaris former base forms fructification young shoot is grown to since Cordyceps militaris former base；
Stage 2：The stage of spore head is initially formed from fruiting bodies of cordyceps militaris height up to 1.5-2cm to fructification；
The thermal stimulation is to make the temperature on daytime higher 5- than the temperature in evening in the range of not departing from Cordyceps militaris growth temperature
6℃；
The stage 1 is that Cordyceps militaris aerial hyphae annesl is completed and swept out after aerial hyphae 3-5 days, and Chinese caterpillar fungus culture medium surface can
See on aerial hyphae when initially forming fruit body primordium, starts thermal stimulation.
2. the method according to claim 1 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that swept with old mycelia remover tool
Aerial hyphae is opened, and aerial hyphae is made to lodge on culture medium in the same direction.
3. the method for cultivating Cordyceps militaris using thermal stimulation according to claim 1 to 2 any one of them, which is characterized in that institute
It is 7-10 days to state 1 duration of stage.
4. the method according to claim 1 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that the stage 2 is
Fruiting bodies of cordyceps militaris height reaches 1.5-2cm, and starts to expand at the top of long white point to fructification at the top of fructification and powder occur
Stage.
5. the method according to claim 4 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that for the stage
2, when fruiting bodies of cordyceps militaris young shoot height reaches 1.5-2cm, and the fructification of long white point reaches 50% or more, start temperature difference thorn
Swash.
6. the method according to claim 1 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that in the Cordyceps militaris stage
1 and the stage 2 cultivation in, it is 18-25 DEG C to make the temperature on daytime, and the temperature in evening is 15-20 DEG C, and day and night temperature is made to be 5-6
℃。
7. the method according to claim 6 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that when day alternates with night
Between point be 7:30 and 17:30.
8. the method according to claim 1 for cultivating Cordyceps militaris using thermal stimulation, which is characterized in that in the Cordyceps militaris stage
1 and the stage 2 cultivation in, light application time 15-19 hours, intensity of illumination 100-150 Luxs.
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