CN111328635A

CN111328635A - Method for cultivating imitated wild gastrodia elata strains in Changbai mountain

Info

Publication number: CN111328635A
Application number: CN202010233583.8A
Authority: CN
Inventors: 李玉梅; 姜云天; 刘汉准
Original assignee: Jilin Normal University
Current assignee: Jilin Normal University
Priority date: 2020-03-26
Filing date: 2020-03-26
Publication date: 2020-06-26

Abstract

The invention discloses a method for cultivating imitated wild gastrodia elata strains in Changbai mountains, which comprises the following steps of 1, selecting strains, picking up waste twigs with the diameter of 1-10 cm in a forest area from winter to felling, cutting the twigs into small wood sections with the length of 10-25 cm for later use, 2, collecting wild strains, selecting and collecting red, tender and thick rhizomes with the diameter of 1-10 cm as mother seeds, 3, selecting a strain culture field, selecting the felled forest area as a strain culture field, 4, culturing the strains, mixing the collected roots with wild armillaria mellea strains and the small wood sections according to a ratio of 1: 10, stacking the mixed small wood sections with the width of 1m × and the height of 30cm in a flat groove, and arranging the cultured small wood sections as the branches for culturing and cultivating gastrodia elata stick, wherein the cultured gastrodia elata mycelium is thick and has strong growth, the cultured gastrodia elata strains are large in size and good quality, and the gastrodia elata round shape is fat.

Description

Method for cultivating imitated wild gastrodia elata strains in Changbai mountain

Technical Field

The invention relates to the technical field of strain cultivation, in particular to a method for cultivating a Changbai mountain wild-like gastrodia elata strain.

Background

Gastrodia elata Blume (Gastrodia elata Blume) is a perennial saprophytic plant of Gastrodia elata of Orchidaceae, is a famous and precious traditional Chinese medicinal material in China, and is recorded in Shen nong Ben Cao Jing for the earliest time. It is sweet in nature and slightly warm, has effects of relieving convulsion, eliminating dizziness, calming endogenous wind, and can be used for treating giddiness, headache, hypertension, limbs anesthesia, neurasthenia, insomnia, rheumatic lumbago and skelalgia, etc. Because the rhizoma gastrodiae extract has no root and no green leaf to absorb and produce nutrition, the rhizoma gastrodiae extract can grow and develop only by symbiosis with the armillaria mellea, and therefore, the quality of the armillaria mellea strain directly influences the quality and the yield of the rhizoma gastrodiae. The growth of the armillaria mellea mycelium is influenced by a plurality of factors such as environmental temperature, growth matrix, culture algebra and the like, the source area of the armillaria mellea strain used for artificially culturing the gastrodia elata in the Changbai mountain area and the culture method are relatively mixed, and the problems of degeneration of the armillaria mellea strain, poor environmental adaptability and the like exist.

Therefore, how to culture the armillaria mellea strain with strong activity and adaptation to the unique climatic environment of the Changbai mountain is a key technology for improving the yield of the wild rhizoma gastrodiae in the Changbai mountain.

Disclosure of Invention

The invention aims to provide a wild-imitating gastrodia elata strain cultivation method adapting to the unique climate environment of Changbai mountain, and aims to solve the problems in the background technology.

In order to solve the technical problems, the invention provides the following technical scheme, and the technical scheme of the invention is a method for cultivating the imitated wild gastrodia elata strain in Changbai mountain, which comprises the following steps:

step 1: selecting a fungus material: picking up waste branches with the diameter of 1-10 cm in a forest area from winter to felling, and cutting the branches into small wood sections with the length of 10-25 cm for later use; winter refers to 11 months-3 months of the next year; the small wood section cut is preferably in a wedge shape;

step 2: collecting wild strains: searching tree roots with a large amount of natural armillaria mellea growing under tree stumps in forest areas harvested from late ten days of 4 months to early ten days of 5 months to the Changbai mountain, and selecting and collecting red, tender and robust tree roots with diameters of 1-10 cm and mycorrhiza as mother seeds; the forest area after felling is the forest area 4-5 years after felling;

step 3, selecting a strain culture field, namely selecting a harvested forest area as the strain culture field, flattening the selected field to form a land block with the width of 2m × and the length of 10m, and stripping the weed leaves and humus with the thickness of 2cm from the ground to one side for later use;

step 4, strain culture, namely mixing the collected roots with the wild Armillaria mellea strain with kg units with small wood sections according to a ratio of 1: 10, stacking the mixed small wood sections according to the width of 1m × and the height of 30cm in a flat groove, filling gaps among the small wood sections with live loess, covering the loess with the thickness of 2cm, covering the loess with weeds, leaves, humus and the like on the flat ground, and culturing for one year to obtain the cultured small wood sections called as fungus branches which can be used for fungus stick culture and gastrodia cultivation.

Compared with the prior art, the invention has the following beneficial effects: the roots of the trees infected with the natural armillaria mellea in the Changbai mountain forest area are directly taken as the ' mother seeds ', the culture field completely simulates the growth environment of the wild armillaria mellea, so that the ' mother seeds ' propagated ' strains (fungus branches) have stout strongylike and strong vitality and are very close to the growth habit of the wild armillaria mellea, and the gastrodia elata cultivated by the strains has large and round body, fleshy texture and good quality.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

FIG. 1 is a flow chart of the method of the present invention

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1, the present invention provides a cultivation method of a Changbai mountain imitating wild rhizoma gastrodiae strain, comprising: the method comprises the following steps:

step 1: selecting a fungus material: picking up waste tree branches in forest areas from 11 months to 3 months in the next year to fell in winter, generally selecting branches with the diameter of 1-10 cm, cutting the branches into small wood sections with the length of 10-25 cm for later use, and conveying the small wood sections to a culture field for later use;

step 2: collecting wild strains: in the forest area from 4 to 5 years after harvesting in Changbai mountain to 4 to 5 years in the last ten days of 4 months, the roots of a large number of natural armillaria mellea growing under the stumps are searched, tender and thick roots with the diameter of 1 to 10cm and red rhizomorph are selected and collected as mother seeds, and areas with proper conditions can be propagated by using local materials.

And 3, selecting a strain culture field, namely selecting a harvested needle-broad mixed forest or mixed wood forest area as the strain culture field, leveling the selected field to form a land block with the width of 2m × and the length of 10m, and stripping the weed leaves and humus with the thickness of 2cm from the ground to one side for later use.

Step 4, strain cultivation, namely cutting branches into small wood sections of 10-25 cm, wherein the cut openings are preferably wedge-shaped, mixing the collected roots with wild Armillaria mellea strains and the small wood sections according to a ratio of 1: 10 (unit kg), stacking the mixed small wood sections according to the width of 1m × and the height of 30cm in a flat groove, filling gaps among the small wood sections with live loess, covering the loess with the thickness of 2cm, covering the loess with weeds, leaves, humus and the like on the flat ground, and culturing for one year (the cultured small wood sections are called as 'fungus branches'), so that the method can be used for fungus stick cultivation, cultivation production and gastrodia elata cultivation.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Changbai mountain wild-imitating gastrodia elata strain cultivation method is characterized by comprising the following steps:

step 1: selecting a fungus material: picking up waste branches with the diameter of 1-10 cm in a forest area from winter to felling, and cutting the branches into small wood sections with the length of 10-25 cm for later use;

step 2: collecting wild strains: searching tree roots with a large amount of natural armillaria mellea growing under tree stumps in forest areas harvested from late ten days of 4 months to early ten days of 5 months to the Changbai mountain, and selecting and collecting red, tender and robust tree roots with diameters of 1-10 cm and mycorrhiza as mother seeds;

step 3, selecting a strain culture field, namely selecting a felled forest area as the strain culture field, leveling the selected field to form a land with the width of 2m × and the length of 10m, and stripping the weed leaves and humus with the thickness of 2cm from the ground to one side for later use;

2. The method according to claim 1, wherein in step 1, the winter season is 11 months-3 months of the next year; the small wood section cut is wedge-shaped.

3. The method according to claim 1, wherein in the step 2, the forest area after harvesting is a forest area 4-5 years after harvesting.

4. The method as claimed in claim 1, wherein in step 3, the culture site is harvested conifer hybridization forest or mixed wood forest area.