CN108967040B - Method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area - Google Patents

Method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area Download PDF

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CN108967040B
CN108967040B CN201811157158.4A CN201811157158A CN108967040B CN 108967040 B CN108967040 B CN 108967040B CN 201811157158 A CN201811157158 A CN 201811157158A CN 108967040 B CN108967040 B CN 108967040B
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polyporus umbellatus
walnut
umbellatus
planting
pond
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CN108967040A (en
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李树红
张小雷
王丽
王晖
李勇军
楼根柱
苏开美
何俊
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Yuxi Shengkang Biotechnology Development Co ltd
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Yuxi Shengkang Biotechnology Development Co ltd
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for pruning branches and planting polyporus umbellatus in space under trees by using walnut forest in Yunnan area, which mainly comprises the following steps: (1) preparing a culture medium for culturing the Armillaria mellea walnut branches; (2) preparing and culturing a culture of the halimasch walnut branches; (3) selecting land and establishing a polyporus umbellatus pond; (4) preparing materials; (5) discharging and planting the grifola frondosa; (6) and (3) carrying out planting management and harvesting on polyporus umbellatus under walnut trees. The method is simple and convenient to operate, can effectively improve the land utilization rate, develops a new fungus material source, protects the ecological environment and promotes the healthy development of the grifola industry.

Description

Method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area
Technical Field
The invention relates to the technical field of medicinal fungus planting, in particular to a method for planting polyporus umbellatus in a simulated wild mode by utilizing walnut forests to prune branches and an under-forest space in Yunnan areas.
Background
Polyporus umbellatus (Pers.) Fries) is a perennial fungus used as crude drug in Basidiomycota, Polyporaceae and Polyporus, and is recorded in Shennong Ben Cao Jing, which has a medicinal history of more than two thousand years, and the sclerotium has the curative effects of inducing diuresis, excreting dampness and reducing swelling. Modern researches show that the polyporus umbellatus polysaccharide has the effects of improving immunity, resisting tumor and aging, treating hepatitis B and the like, and has good application and development prospects. Polyporus umbellatus must be symbiotic with Armillaria mellea, and can grow and reproduce after digesting Armillaria mellea to obtain nutrition, and cannot grow without Armillaria mellea. Armillaria mellea mainly depends on broadleaf tree sections to provide nutrition for the Armillaria mellea. With the annual expansion of the planting area of the polyporus umbellatus, a large number of trees need to be felled, which causes serious damage to the ecological environment. In addition, the traditional planting of the polyporus umbellatus in the Yunnan area needs two steps of culturing the fungus material (fungus pond) and planting the polyporus umbellatus, so that the time and the labor are wasted, the labor amount is large, and the labor cost is high. Therefore, the search for the umbellate pore fungus planting technology which saves labor cost and replaces fungus materials is urgently needed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for planting polyporus umbellatus by utilizing walnut forest to prune branches and under-forest spaces in Yunnan, the operation is simple and convenient, the land utilization rate can be effectively improved, a new fungus material source is developed, the ecological environment is protected, and the healthy development of the polyporus umbellatus industry is promoted.
A method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area comprises the following steps:
s1, mixing sawdust, walnut branch and bran uniformly, adding gypsum powder and KH2PO4、MgSO4Mixing sucrose and water uniformly, adding water until the water content of the substrate is 55-65%, stacking and fermenting for 2-3 d to obtain a substrate for the growth of the symbiotic armillaria mellea of the polyporus umbellatus for later use; sawdust, walnut branch, bran, gypsum powder and KH2PO4、MgSO4The proportion of the cane sugar is 65-75 percent of sawdust, 5-10 percent of walnut branches, 15-20 percent of bran, 1-2 percent of gypsum powder and KH2PO4 0.5%~1%、MgSO40.5-1 percent of sucrose and 1-2 percent of sucrose;
s2, filling the substrate obtained in the step S1 into a container, covering the container, sterilizing, cooling to room temperature, inoculating polyporus umbellatus symbiotic armillaria mellea stock, then placing the container in a strain culture room at 23-25 ℃ for culturing for 50-60 days, and obtaining an armillaria mellea walnut branch culture strain for later use after the armillaria mellea rope grows over the container;
s3, selecting loam with the perennial average temperature of 9-16 ℃, the air relative humidity of 65-90%, the altitude of 1200-3000 m, the mountain slope of 5-30 ℃ and the pH of 6.5, and establishing a polyporus umbellatus fungi pond in a walnut forest with the walnut tree planting age of 4 years or more;
s4, selecting fresh walnut trees with the diameter of 5-10 cm to prune branches, airing for 3-5 d, removing twigs, sawing into large wood sections with the length of 15-20 cm, cutting 1 fish scale opening at intervals of 7-8 cm on one side of each large wood section, wherein the depth of each fish scale opening reaches the xylem, simultaneously cutting the fresh walnut trees with the diameter of 2-3 cm into small wood sections with the length of 5-10 cm, and cutting two ends of each small wood section into splayed inclined planes;
s5, in late April, paving a layer of walnut withered leaves or broad-leaved tree withered leaves at the bottom of the agaric pond dug in the step S3, placing large wood sections obtained by the processing in the step S4 on the paved leaves, covering soil to compact gaps among the wood sections, uniformly placing the umbellate pore fungus on the end head and the fish scale opening of the large wood section, simultaneously uniformly placing the armillaria mellea twig cultivation strains on two sides of the umbellate pore fungus, then scattering a layer of small wood sections obtained by the processing in the step S4, filling gaps with soil, and covering a layer of walnut withered leaves or broad-leaved tree withered leaves after covering the soil;
s6, digging the Polyporus umbellatus in the middle and last ten days of 11 months of the 3 rd year after the completion of the step S5, digging out the Polyporus umbellatus and the Polyporus umbellatus, remaining the Polyporus umbellatus in the planting pond, backfilling soil, and continuing to grow.
Further, in step S1, the sawdust is obtained by cutting and crushing walnut branches; the walnut branches are short twigs which are obtained by cutting walnut pruning branches with the diameter of 2-3 cm into 3-4 cm.
Further, the specific process of step S2 is:
2 months before the step S5, filling the substrate obtained in the step S1 into a 750mL polypropylene plastic bottle, compacting, filling the substrate to a height of 4/5 of the polypropylene plastic bottle, and covering the polypropylene plastic bottle; sterilizing a polypropylene plastic bottle filled with the matrix under high pressure for 2 to 3 hours or sterilizing the polypropylene plastic bottle under normal pressure for 24 to 28 hours at the temperature of 121 ℃, then cooling the polypropylene plastic bottle to room temperature, opening the bottle mouth, inoculating the stock strain of the polyporus umbellatus symbiotic armillaria, and culturing the polypropylene plastic bottle inoculated with the stock strain of the polyporus umbellatus symbiotic armillaria in a strain culture room at the temperature of 23 to 25 ℃ for 50 to 60 days for later use.
Further, in step S3, the agaric fungus pond is built from the middle and last ten days of 10 months to the last ten days of 11 months, the agaric fungus pond is dug along the slope direction 50cm away from the walnut tree, the length of the agaric fungus pond is along the slope direction, and the width is along the contour line; digging soil at the bottom of the polyporus umbellatus pond for loosening by 3-5 cm, making the bottom of the polyporus umbellatus pond into an inclined plane of 5-10 degrees along the slope direction, and leaving a sidewalk of 50-60 cm between the polyporus umbellatus ponds.
Furthermore, the agaric fungus pond is 60cm to 70cm long, 40cm to 50cm wide and 20cm to 25cm deep.
Further, step S4 is performed after the walnut is harvested and before the leaves turn yellow, i.e., in the middle and last 10 months.
Further, the specific flow of step S5 is:
in the last 11 th month, paving a layer of walnut tree dead leaves or broad-leaved tree dead leaves which are soaked in water and drained of clear water and have the thickness of 0.5 cm-1 cm at the bottom of the dug polyporus umbellatus pond, placing 6-8 big wood sections on the paved leaves, and horizontally placing the large wood sections with fish scales facing to the top; the distance between the sections of two adjacent large wood sections is 2 cm-3 cm, the distance between the large wood sections is 8 cm-10 cm, and the placing direction of the large wood sections is parallel to the contour line; filling gaps among the large wood sections with soil to the fish scale openings, and compacting and filling the soil;
selecting grifola which is free of scars, insects and diseases, has normal color and elasticity, is white or light yellow when being broken off and has the weight of 20-35 g as grifola, and uniformly placing the grifola at the end of a log section and the fish scale mouth; cutting each bottle of Armillaria mellea branch cultivation strains into 10-15 pieces with uniform size, respectively placing one Armillaria mellea branch cultivation strain on each side of the Polyporus umbellatus, laying the Polyporus umbellatus and the Armillaria mellea branch cultivation strains, scattering a layer of small wood section with the thickness of 3-5 cm obtained in the step S4, filling the vacant space with soil, covering the soil with 10-15 cm, and finally covering a layer of walnut tree withered branches or broad leaf tree withered leaves.
Further, after the step S5 is completed and before the step S6, the polyporus umbellatus pond is prohibited to be trampled, and the walnut tree branches and leaves are used for covering the pond to reduce water evaporation, keep the soil moist and prevent the soil hardening and freezing damage caused by rain wash; in addition, a drainage ditch is dug to prevent water from accumulating in the agaric fungus pond.
Further, in step S6, digging the polyporus umbellatus in the middle and last ten days of 11 months of the 3 rd year after planting, digging out the polyporus umbellatus and the polyporus umbellatus, leaving the polyporus umbellatus in the planting pond, backfilling the soil, and continuing to grow; the collected sclerotium of Polyporus umbellatus is classified according to individual size, Grifola umbellatus is used for cultivating Polyporus umbellatus, and Polyporus umbellatus is used as commercial Polyporus umbellatus.
The invention has the beneficial effects that:
the Yunnan walnut species have rich resources and long cultivation history, and are the origin and main distribution area of the world deep-line walnuts. According to the incomplete statistics of 2017, the planting area of the walnuts in Yunnan province reaches 4280 ten thousand mu, walnuts can be planted in the counties (cities and districts) which are more than 90% of the first province of China, the area which is already put into production is more than 1000 ten thousand mu, and a large number of walnut pruning branches are arranged every year.
Firstly, the walnut forest idle open space is selected to simulate the growing environment of the polyporus umbellatus to provide proper conditions of temperature, illumination, moisture and the like for the growth of the polyporus umbellatus, so that the utilization rate of land resources is improved;
secondly, the branches of the walnut forest are trimmed to prepare the culture strains and the fungus materials of the halimasch walnut branches, a new fungus material source is developed, a large amount of forest resources can be saved, a large amount of agricultural and forestry waste materials are utilized, and the existence of waste is reduced. The honey fungus walnut branch cultivated species are cut into blocks with uniform size and are placed on two sides of the polyporus umbellatus, the symbiotic relation among the cut-log, honey fungus and polyporus umbellatus is coordinated, the fungus material does not need to be cultured in advance, the manpower and the time for culturing the fungus material are saved, and the growth cycle of the polyporus umbellatus is shortened and the labor cost is reduced.
Compared with the traditional planting method of the polyporus umbellatus, the method is simple and convenient to operate and low in cost, the planting period of the polyporus umbellatus can be effectively shortened, the ecological environment is protected, and the healthy development of the polyporus umbellatus industry is promoted.
Detailed Description
The present invention will be further described below, and it should be noted that the present embodiment is based on the technical solution, and a detailed implementation manner and a specific operation process are provided, but the protection scope of the present invention is not limited to the present embodiment.
Example 1
A method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area comprises the following steps:
s1, mixing sawdust, walnut branch and bran uniformly, adding gypsum powder and KH2PO4、MgSO4Mixing sucrose and water uniformly, adding water until the water content of the substrate is 55-65%, stacking and fermenting for 2-3 d to obtain a substrate for the growth of the symbiotic armillaria mellea of the polyporus umbellatus for later use; sawdust, walnut branch, bran, gypsum powder and KH2PO4、MgSO4The cane sugar comprises 65 percent of sawdust, 10 percent of walnut branches, 20 percent of bran, 1 percent of gypsum powder and KH2PO4 1%、MgSO41% and 2% of cane sugar;
s2, filling the substrate obtained in the step S1 into a polypropylene plastic bottle, covering the plastic bottle, sterilizing, cooling to room temperature, inoculating polyporus umbellatus symbiotic armillaria mellea stock, then placing the stock in a strain culture room at 23-25 ℃ for culturing for 50-60 d, and obtaining an armillaria mellea branch culture strain for later use after the armillaria mellea strain grows full of the plastic bottle;
s3, selecting loam with the perennial average temperature of 9-16 ℃, the air relative humidity of 65-90%, the altitude of 1200-3000 m, the mountain slope of 5-30 ℃ and the pH of about 6.5, and establishing a polyporus umbellatus pond with the length of 60-70 cm, the width of 40-50 cm and the depth of 20-25 cm in a walnut forest where walnut trees are planted for 4 years or more in free land;
s4, selecting fresh walnut trees with the diameter of 5-10 cm to prune branches, airing for 3-5 d, removing twigs, sawing into large wood sections with the length of 15-20 cm, cutting 1 fish scale opening at intervals of 7-8 cm on one side of each large wood section, wherein the depth of each fish scale opening reaches the xylem, simultaneously cutting the fresh walnut trees with the diameter of 2-3 cm into small wood sections with the length of 5-10 cm, and cutting two ends of each small wood section into splayed inclined planes;
s5, in last 11 th day, paving a layer of walnut tree withered leaves or broad leaf tree withered leaves at the bottom of the agaric pond dug in the step S3, placing 6-8 large wood sections obtained by the processing in the step S4 on the paved leaves, covering soil to compact gaps among the wood sections, uniformly placing the agaric seeds on the ends and the fish scale mouths of the large wood sections, simultaneously uniformly placing the Armillaria mellea walnut branch cultivation strains on two sides of the agaric seeds, then spreading a layer of small wood sections obtained by the processing in the step S4, filling the gaps with soil, covering the soil with the thickness of 10-15 cm, and then covering a layer of walnut tree branches and leaves or broad leaf tree withered leaves;
s6, prohibiting treading; the dead branches and leaves of the walnut trees are used for covering the pond to reduce water evaporation, keep the soil moist and prevent the soil hardening and freezing damage caused by rain wash; digging a drainage ditch to prevent water from accumulating in the agaric fungus pond; digging Polyporus umbellatus in middle and last ten days of 11 months of 3 years after planting, digging out Polyporus umbellatus and Polyporus umbellatus, placing Polyporus umbellatus in planting pond, backfilling soil, and continuing to grow.
Further, in step S1, the sawdust is obtained by cutting and crushing walnut branches; the walnut branches are short twigs which are obtained by cutting walnut pruning branches with the diameter of 2-3 cm into 3-4 cm.
Further, the specific process of step S2 is:
2 months before the step S5, filling the substrate obtained in the step S1 into a 750mL polypropylene plastic bottle, compacting, filling the substrate to a height of 4/5 of the polypropylene plastic bottle, and covering the polypropylene plastic bottle; sterilizing a polypropylene plastic bottle filled with the matrix under high pressure for 2 to 3 hours or sterilizing the polypropylene plastic bottle under normal pressure for 24 to 28 hours at the temperature of 121 ℃, then cooling the polypropylene plastic bottle to room temperature, opening the bottle mouth, inoculating the stock strain of the polyporus umbellatus symbiotic armillaria, and culturing the polypropylene plastic bottle inoculated with the stock strain of the polyporus umbellatus symbiotic armillaria in a strain culture room at the temperature of 23 to 25 ℃ for 50 to 60 days for later use.
Further, in step S3, the agaric fungus pond is built from the middle and last ten days of 10 months to the last ten days of 11 months, the agaric fungus pond is dug along the slope direction 50cm away from the walnut tree, the length of the agaric fungus pond is along the slope direction, and the width is along the contour line; digging soil at the bottom of the polyporus umbellatus pond for loosening by 3-5 cm, making the bottom of the polyporus umbellatus pond into an inclined plane of 5-10 degrees along the slope direction, and leaving a sidewalk of 50-60 cm between the polyporus umbellatus ponds.
Further, step S4 is performed after the walnut is harvested and before the leaves turn yellow, i.e., in the middle and last 10 months.
Further, the specific flow of step S5 is:
in the last 11 th month, paving a layer of walnut tree dead leaves or broad-leaved tree dead leaves which are soaked in water and drained of clear water and have the thickness of 0.5 cm-1 cm at the bottom of the dug polyporus umbellatus pond, placing 6-8 big wood sections on the paved leaves, and horizontally placing the large wood sections with fish scales facing to the top; the distance between the sections of two adjacent large wood sections is 2 cm-3 cm, the distance between the large wood sections is 8 cm-10 cm, and the placing direction of the large wood sections is parallel to the contour line; filling gaps among the large wood sections with soil to the fish scale openings, and compacting and filling the soil;
selecting grifola which is free of scars, insects and diseases, has normal color and elasticity, is white or light yellow when being broken off and has the weight of 20-35 g as grifola, and uniformly placing the grifola at the end of a log section and the fish scale mouth; cutting each bottle of Armillaria mellea branch cultivation strains into 10-15 pieces with uniform size, respectively placing one Armillaria mellea branch cultivation strain on each side of the Polyporus umbellatus, laying the Polyporus umbellatus and the Armillaria mellea branch cultivation strains, scattering a layer of small wood section with the thickness of 3-5 cm obtained in the step S4, filling the vacant space with soil, covering the soil with 10-15 cm, and finally covering a layer of walnut tree withered branches or broad leaf tree withered leaves.
Further, step S6 is specifically:
stepping on a polyporus umbellatus pond when walnut picking is forbidden; collecting walnut dead branches and leaves to cover the fungus pond so as to reduce water evaporation, keep soil moist and prevent soil hardening and freezing damage caused by rain wash; before a rainy season comes, a drainage ditch is dug in advance to prevent water from accumulating in the pond; digging Polyporus umbellatus in the middle and last ten days of 11 months of the 3 rd year after planting, digging out Polyporus umbellatus and Polyporus umbellatus, placing Polyporus umbellatus in the planting pond, backfilling soil, and continuing to grow; the collected sclerotium of Polyporus umbellatus is classified according to individual size, Grifola umbellatus is used for cultivating Polyporus umbellatus, and Polyporus umbellatus is used as commercial Polyporus umbellatus.
Example 2
This example is substantially the same as example 1, with the main difference being that in this example, sawdust, walnut branches, bran, gypsum powder, KH2PO4、MgSO4The sucrose comprises 75 percent of sawdust, 5 percent of walnut branches, 15 percent of bran, 2 percent of gypsum powder and KH2PO4 1%、MgSO41% and 1% of cane sugar.
Example 3
This example and implementationExample 1 is essentially the same, the main difference being that in this example, sawdust, walnut branches, bran, gypsum powder, KH2PO4、MgSO4The sucrose comprises 70 percent of sawdust, 8 percent of walnut branches, 18 percent of bran, 1 percent of gypsum powder and KH2PO4 0.5%、MgSO40.5 percent and 2 percent of cane sugar.
Various corresponding changes and modifications can be made by those skilled in the art based on the above technical solutions and concepts, and all such changes and modifications should be included in the protection scope of the present invention.

Claims (8)

1. A method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area is characterized by comprising the following steps:
s1, mixing sawdust, walnut branch and bran uniformly, adding gypsum powder and KH2PO4、MgSO4Mixing sucrose and water uniformly, adding water until the water content of the substrate is 55-65%, stacking and fermenting for 2-3 d to obtain a substrate for the growth of the symbiotic armillaria mellea of the polyporus umbellatus for later use; sawdust, walnut branch, bran, gypsum powder and KH2PO4、MgSO4The proportion of the cane sugar is 65-75 percent of sawdust, 5-10 percent of walnut branches, 15-20 percent of bran, 1-2 percent of gypsum powder and KH2PO40.5%~1%、MgSO40.5-1 percent of sucrose and 1-2 percent of sucrose;
s2, filling the substrate obtained in the step S1 into a container, covering the container, sterilizing, cooling to room temperature, inoculating polyporus umbellatus symbiotic armillaria mellea stock, then placing the container in a strain culture room at 23-25 ℃ for culturing for 50-60 days, and obtaining an armillaria mellea walnut branch culture strain for later use after the armillaria mellea rope grows over the container;
s3, selecting loam with the perennial average temperature of 9-16 ℃, the air relative humidity of 65-90%, the altitude of 1200-3000 m, the mountain slope of 5-30 ℃ and the pH of 6.5, and establishing a polyporus umbellatus pond in a walnut forest with the walnut tree planting age of more than 4 years;
s4, selecting fresh walnut trees with the diameter of 5-10 cm to prune branches, airing for 3-5 d, removing twigs, sawing into large wood sections with the length of 15-20 cm, cutting 1 fish scale opening at intervals of 7-8 cm on one side of each large wood section, wherein the depth of each fish scale opening reaches the xylem, simultaneously cutting the fresh walnut trees with the diameter of 2-3 cm into small wood sections with the length of 5-10 cm, and cutting two ends of each small wood section into splayed inclined planes;
s5, in the last ten days of 11 months, paving a layer of walnut withered leaves which are 0.5-1 cm thick and soaked with water and drained of clear water at the bottom of the dug polyporus umbellatus pond, and placing 6-8 big wood sections on the paved leaves, wherein the scales of the big wood sections are horizontally placed; the distance between the sections of two adjacent large wood sections is 2 cm-3 cm, the distance between the large wood sections is 8 cm-10 cm, and the placing direction of the large wood sections is parallel to the contour line; filling gaps among the large wood sections with soil to the fish scale openings, and compacting and filling the soil;
selecting grifola which is free of scars, insects and diseases, has normal color and elasticity, is white or light yellow when being broken off and has the weight of 20-35 g as grifola, and uniformly placing the grifola at the end of a log section and the fish scale mouth; cutting each bottle of Armillaria mellea branch cultivation strains into 10-15 pieces with uniform size, respectively placing one Armillaria mellea branch cultivation strain on each side of the Polyporus umbellatus, laying the Polyporus umbellatus and the Armillaria mellea branch cultivation strains, scattering a layer of small wood section with the thickness of 3-5 cm obtained in the step S4, filling the vacant space with soil, covering the soil with 10-15 cm, and finally covering a layer of walnut tree withered leaves;
s6, digging the Polyporus umbellatus in the middle and last ten days of 11 months of the 3 rd year after the completion of the step S5, digging out the Polyporus umbellatus and the Polyporus umbellatus, remaining the Polyporus umbellatus in the planting pond, backfilling soil, and continuing to grow.
2. The method for growing polyporus umbellatus by pruning branches and under-forest spaces with walnut forest in Yunnan area according to claim 1, wherein in step S1, the sawdust is obtained by crushing the pruned branches with walnut; the walnut branches are short twigs which are obtained by cutting walnut pruning branches with the diameter of 2-3 cm into 3-4 cm.
3. The method for pruning branches and planting polyporus umbellatus in the space under the forest by using the walnut forest in the Yunnan area according to claim 1, wherein the specific process of the step S2 is as follows:
2 months before the step S5, filling the substrate obtained in the step S1 into a 750mL polypropylene plastic bottle, compacting, filling the substrate to a height of 4/5 of the polypropylene plastic bottle, and covering the polypropylene plastic bottle; sterilizing a polypropylene plastic bottle filled with the matrix under high pressure for 2 to 3 hours or sterilizing the polypropylene plastic bottle under normal pressure for 24 to 28 hours at the temperature of 121 ℃, then cooling the polypropylene plastic bottle to room temperature, opening the bottle mouth, inoculating the stock strain of the polyporus umbellatus symbiotic armillaria, and culturing the polypropylene plastic bottle inoculated with the stock strain of the polyporus umbellatus symbiotic armillaria in a strain culture room at the temperature of 23 to 25 ℃ for 50 to 60 days for later use.
4. The method for pruning branches and planting polyporus umbellatus in the understory space by using walnut forest in the Yunnan area according to claim 1, wherein in step S3, the time for establishing the polyporus umbellatus pond is from the middle and last ten days of 10 months to the last ten days of 11 months, the polyporus umbellatus pond is dug along the slope direction 50cm away from the walnut tree, and the length and the width of the polyporus umbellatus pond are along the slope direction and the contour line; digging soil at the bottom of the polyporus umbellatus pond for loosening by 3-5 cm, making the bottom of the polyporus umbellatus pond into an inclined plane of 5-10 degrees along the slope direction, and leaving a sidewalk of 50-60 cm between the polyporus umbellatus ponds.
5. The method for pruning branches and planting polyporus umbellatus in the space under the forest by using the walnut forest in the Yunnan area according to claim 4, wherein the polyporus umbellatus pond is 60 cm-70 cm long, 40 cm-50 cm wide and 20 cm-25 cm deep.
6. The method for pruning branches and planting polyporus umbellatus in the forest according to the walnut forest in the Yunnan area as claimed in claim 1, wherein step S4 is performed after the walnut is harvested and before the leaves turn yellow, i.e. in the middle and last 10 months.
7. The method for planting polyporus umbellatus by pruning branches and understory spaces with walnut forest in Yunnan area according to claim 1, wherein after step S5 is completed and before step S6, the stepping on polyporus umbellatus pond is prohibited, and walnut tree branches and leaves are used for covering the pond to reduce water evaporation, keep the soil moist, and prevent soil hardening and freezing damage caused by rain wash; in addition, a drainage ditch is dug to prevent water from accumulating in the agaric fungus pond.
8. The method for pruning branches and planting polyporus umbellatus in the understory space by using walnut forest in the Yunnan area according to claim 1, wherein in step S6, digging polyporus umbellatus in the middle and last ten days of 11 months of 3 years after planting, digging out black polyporus umbellatus and grifola umbellatus, leaving the grifola umbellatus in a planting pond, backfilling soil, and continuing to grow; the collected sclerotium of Polyporus umbellatus is classified according to individual size, Grifola umbellatus is used for cultivating Polyporus umbellatus, and Polyporus umbellatus is used as commercial Polyporus umbellatus.
CN201811157158.4A 2018-09-30 2018-09-30 Method for pruning branches and planting polyporus umbellatus in space under walnut forest in Yunnan area Active CN108967040B (en)

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