CN110499245B - Microalgae in-situ expansion culture system for shellfish culture pond and use method thereof - Google Patents

Microalgae in-situ expansion culture system for shellfish culture pond and use method thereof Download PDF

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CN110499245B
CN110499245B CN201910731388.5A CN201910731388A CN110499245B CN 110499245 B CN110499245 B CN 110499245B CN 201910731388 A CN201910731388 A CN 201910731388A CN 110499245 B CN110499245 B CN 110499245B
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water
microalgae
seawater
situ
mother liquor
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CN110499245A (en
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焦海峰
金信飞
郑梓元
林志华
尤仲杰
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Zhejiang Wanli University
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Abstract

The invention discloses a microalgae in-situ expansion culture system for a shellfish culture pond, which comprises a water lifting device, a nutrient salt supply device, a microalgae expansion device and a seawater in-situ filtering device, wherein the seawater in-situ filtering device is arranged on the seawater in-situ filtering device; the seawater in-situ filtering device comprises a water filtering tank, a water outlet pipeline and a water storage tank, wherein the water filtering tank is filled with water filtering sponge, and one side of the top end of the water filtering tank is provided with a water collecting port; the water lifting device comprises a lower water lifting pipe, a water lifting pump and an upper water lifting pipe which are connected in sequence; the nutrient salt supply device comprises a mother liquor extraction pipe and a mother liquor bottle, and the mother liquor bottle is communicated with the lower water lifting pipe through the mother liquor extraction pipe; the microalgae amplification device comprises a microalgae discharge pipe and a reactor box body, wherein a microalgae liquid overflow port is formed in the side wall of the reactor box. Through the use of the microalgae in-situ expansion culture system, the circulation of in-situ extraction of seawater in the shellfish culture pond, auxiliary injection of nutrient solution, high-order microalgae expansion culture and continuous supply of algae solution is realized, and the continuous supply of bait organisms is increased.

Description

Microalgae in-situ expansion culture system for shellfish culture pond and use method thereof
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a microalgae in-situ expansion culture system for a shellfish culture pond and a use method thereof.
Background
Pond culture is one of the modes of mariculture, and is more and more favored in recent years because the culture environment is relatively controllable, and the comprehensive culture economic benefit is greatly improved compared with an open culture mode. However, due to the differences of environmental characteristics and different dominant cultured varieties, the variety of pond culture in different regions is different, the variety of culture in the open pond is mainly buried shellfish (mainly Sinonovacula constricta, arca subcrenata, and clam) in the eastern coastal region of China, and the variety of culture in part of the pond is matched with the shellfish such as Portunus trituberculatus, scylla paramamosain, and the like, and the variety of culture in part of the pond is sometimes white shrimp, penaeus japonicus, and the like. The aquaculture ponds mainly increase the output capacity of cultured organisms by changing natural seawater or by manually feeding, and the two modes have great limitations: firstly, the way of increasing the output capacity by changing natural seawater is obviously influenced by the bait in the changed water body, if the bait organisms in the changed water body are not abundant, the bait of shellfish in the culture water body is insufficient, the growth is slow, and the economic benefit is not high; secondly, the mode of increasing the output by artificial feeding is mainly used in a pond for mixedly culturing crabs, shrimps and shellfish, the feeding is used for the growth of crabs and shrimps, the residual bait, the excrement and algae which are naturally propagated are used for the growth of shellfish, the mode can cause the pollution of a culture water body, under the condition that the current environmental awareness is increasingly enhanced, the mode is not suitable for large-scale popularization, in addition, the bait organisms in the culture mode are not rich enough, the shellfish culture density is not suitable to be too high, and the output is influenced.
Bivalve shellfish cultivated in ponds are filter feeding and they grow by feeding organic debris and microalgae in the water. Therefore, in order to improve the environment of the shellfish culture pond, accelerate the shellfish growth speed or increase the shellfish culture yield in the pond, besides the conventional water changing and fertilizer water technology, the method for increasing the yield capacity of the pond through exogenous bait supplementing is a more scientific way, so that the method for increasing the yield of the cultured pond is realized through exogenous facilities. At present, no related report is made on the mode of culturing microalgae in situ in a culture pond, and researchers develop photobioreactors for culturing microalgae, but the photobioreactors have some problems and are not suitable for culturing microalgae in situ in a shellfish culture pond: firstly, the photo-biological reactors have smaller volume and are only suitable for micro-algae cultivation in a large scale; secondly, the microalgae culture water is sand-filtered seawater, and water treatment is needed to be carried out in another pond; thirdly, the microalgae supply is not sustainable, and is only intermittent, namely, the bioreactor is used for culturing 1 pool of microalgae, and the secondary culture is carried out after the primary equipment is cleaned again, so that time and labor are wasted. Meanwhile, during shellfish breeding, a cement pond is mostly adopted for cultivating microalgae, but the cultivation mode has high facility degree requirement, can not move, and is not suitable for in-situ cultivation. The device realizes the on-site culture of the microalgae, realizes the in-situ utilization of the seawater in the culture pond through the water lifting device, and simultaneously realizes the primary culture and continuous supply of the microalgae.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the microalgae in-situ expansion culture system for the shellfish culture pond and the use method thereof, which utilize external kinetic energy to realize in-situ utilization of seawater in the culture pond after simple precipitation and filtration, improve the utilization efficiency of water bodies and further realize natural expansion culture of microalgae.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a microalgae in-situ expansion culture system for a shellfish culture pond comprises a water lifting device, a nutrient salt supply device, a microalgae expansion device and a seawater in-situ filtering device arranged in the culture pond; the seawater in-situ filtering device comprises a water filtering tank, a water outlet pipeline and a water storage tank, wherein the water filtering tank is filled with water filtering sponge, a water collecting port is formed in one side of the top end of the water filtering tank, and the opposite side of the bottom end of the water filtering tank is communicated with the water storage tank through the water outlet pipeline; the water lifting device comprises a lower water lifting pipe, a water lifting pump and an upper water lifting pipe which are connected in sequence, and the lower end of the lower water lifting pipe is inserted into the water storage tank; the nutrient salt supply device comprises a mother liquor extraction pipe and a light-tight mother liquor bottle filled with nutrient salt mother liquor, and the mother liquor bottle is communicated with the lower water lifting pipe through the mother liquor extraction pipe; the microalgae amplification device comprises a microalgae discharge pipe and a transparent reactor box body for microalgae culture, wherein the top end of the upper lifting pipe stretches into the reactor box body, and the side wall of the reactor box is provided with a microalgae liquid overflow port for connecting the microalgae discharge pipe.
Preferably, a bolting silk blocking net is fixedly connected above the water collecting port; and a supporting rod for supporting the bolting silk baffle net is arranged below the bolting silk baffle net.
Preferably, the seawater in-situ filtering device further comprises a water baffle I and a water baffle II; the water baffle I is positioned at one side close to the water collecting port, the top end and the side edge of the water baffle I are fixedly connected to the inner surface of the water filtering tank, and the bottom end of the water baffle I is not contacted with the bottom end of the water filtering tank; the water baffle II is positioned on one side close to the water storage tank, the bottom end and the side edge of the water baffle II are fixedly connected to the inner surface of the water filtering tank, and the top end of the water baffle II is not in contact with the top end of the water tank.
Preferably, the bottom end of the lower water lifting pipe is provided with a water lifting protection head for water filtering, which is made of gas stone; the water lifting pump is provided with a flow rate switch.
Preferably, a control valve for controlling the flow rate of nutrient salt mother liquor is arranged on the mother liquor extraction pipe.
Preferably, a detachable reactor upper cover is arranged at the top end of the reactor box body; the lower surface of the reactor upper cover is provided with illumination equipment for providing a light source for microalgae growth; the bottom in the reactor box body is provided with a nano-gas-filled tube.
Preferably, the algae liquid overflow outlet is positioned at one side far away from the water outlet of the upper water lifting pipe, and the height of the algae liquid overflow outlet is lower than that of the water outlet of the upper water lifting pipe; the microalgae amplification device further comprises a mixing baffle plate, wherein the mixing baffle plate is arranged in the reactor box body and divides the reactor box body into two parts, the bottom end of the mixing baffle plate is not contacted with the bottom of the reactor box body, and the mixing baffle plate is close to one side of a water outlet of the water lifting pipe.
Further, the invention also provides a use method of the microalgae in-situ expansion culture system for the shellfish culture pond, which comprises the following specific steps:
(1) According to the difference of the microalgae species to be cultivated, preparing a culture solution required by the expansion cultivation of the microalgae species in advance, and placing the culture solution in a mother liquor bottle for standby;
(2) The algae seeds are spread in advance and then transferred into a reactor box body,
(3) The seawater in-situ filtering device is placed at the bottom of the aquaculture pond, and after the seawater is filtered for the first time by the bolting silk blocking net to block large-scale impurities by means of the natural pressure of the seawater, the seawater enters the water filtering tank from the water collecting port, and flows into the water storage tank for standby after bypassing and filtering along the bottom end and the top end of the water baffle I and the water baffle II in the water filtering sponge;
(4) Starting a power switch of the water lifting pump, opening a control valve on the mother liquor extraction pipe, pumping seawater in the water storage tank and nutrient salt mother liquor in the mother liquor bottle into the reactor box, and injecting amplified algae liquid into the shellfish culture pond through the algae discharging pipe as the seawater is continuously injected and the algae are amplified, so that shellfish ingestion is realized.
Preferably, in the step (2), the initial stage of expanding cultivation of the algal species has a liquid level of 1/5 of the height of the reactor tank.
Preferably, in the step (4), a flow rate switch on the lifting pump is adjusted to control the water inflow into the reactor box; the lighting device can be selectively turned on at night or in cloudy days to increase the light source required for microalgae propagation.
Compared with the prior art, the invention has the advantages that:
(1) The microalgae in-situ expansion culture system can utilize external kinetic energy to realize in-situ utilization of seawater in the culture pond after simple precipitation and filtration, improve the utilization efficiency of water, and further realize natural expansion culture of microalgae.
(2) The microalgae in-situ expansion culture system can realize in-situ culture of microalgae, can realize uninterrupted supply of the microalgae by means of continuous injection of water body by utilizing the characteristic of self-expansion culture of the microalgae, can effectively improve the actual effect of microalgae culture, provides baits for filter-feeding shellfish cultured in a pond, and has the advantages of simple use, higher microalgae expansion culture rate, sufficient bait organism supply and the like.
(3) The microalgae in-situ expansion culture system is placed beside a dam of a shellfish culture pond and is properly fixed, and the system can be used as an independent unit for single body arrangement, and also can be used for simultaneously arranging a plurality of groups of microalgae in-situ expansion culture systems; the size of the microalgae in-situ expansion culture system (such as the size of a water filtering tank, a water storage tank, a mother liquor bottle, a reactor box body and the like) can be properly adjusted, so that the distribution quantity can be freely selected according to the concentration of baits in a water body, the shellfish culture density and the like. After the use, the cultivation environment and cultivation ecology of the pond can be effectively improved.
In summary, through the use of the microalgae in-situ expansion culture system, the circulation of in-situ extraction of seawater in the shellfish culture pond, auxiliary injection of nutrient solution, high-order microalgae expansion culture and continuous supply of algae solution is realized, and continuous supply of bait organisms is increased.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic illustration of a microalgae in situ expansion culture system for a shellfish culture pond in accordance with the present invention;
reference numerals: 1. a bolting silk screen; 2. a water filtering tank; 3. a drainage sponge; 4. a water baffle I; 5. a water storage tank; 6. lifting water to protect the head; 7. a down-lifting pipe; 8. a mother liquor bottle; 9. a mother liquor extraction tube; 10. a control valve; 11. a water lifting pump; 12. mixing the partition plates uniformly; 13. a water lifting pipe is arranged; 14. a water outlet; 15. a reactor upper cover; 16. an algae liquid overflow port; 17. a reactor tank; 18. a nano-gas tube; 19. a water outlet pipe; 20. a water collecting port; 21. a brace rod; 22. a water baffle II; 23. an algae removing pipe; 24. a flow rate switch.
Detailed Description
The invention will be described in further detail with reference to the drawings and the detailed description.
Example 1
As shown in figure 1, the microalgae in-situ amplification system for the shellfish culture pond comprises a seawater in-situ filtering device, a water lifting device, a nutrient salt supply device and a microalgae amplification device. The seawater in-situ filtering device is placed in the shellfish culture pond, the length of the water lifting device (namely a lower water lifting pipe) is controlled, the water lifting device is connected with the seawater in-situ filtering device, the nutrient salt supply device is additionally arranged on the water lifting device, and then the nutrient salt supply device and the seawater in-situ filtering device are placed on the microalgae amplification device together and are fixed. The components of the system are detachable structures, and the components are connected together through screws or fasteners. Hereinafter, the constituent elements of each device will be described one by one.
(1) Seawater in-situ filtering device
The water filtering tank (2): the seawater filter is used for filtering seawater in a pond, and plays roles of water storage and filtration under the assistance of a water filtering sponge. The water collecting port (20) is formed in the top end of the water filtering tank (2), the water collecting port (20) is an inlet for seawater in the culture pond to enter the water filtering tank (2), a water filtering tank water outlet (not shown in the figure) communicated with the water storage tank (5) is formed in the opposite side of the bottom end of the water filtering tank (2), and the water collecting port (20) and the water filtering tank (2) are arranged at the water outlet position so as to prolong the seawater filtering distance and improve the seawater filtering effect.
Bolting silk screen (1): is a cover net made of a bolting silk net, is covered at a water collecting port (20) at the top end of the water filtering tank (2) and is used for preventing objects such as small-sized organisms, garbage and the like in a pond from entering the water filtering tank (2) and plays roles of blocking and primary filtering.
Brace (21): is positioned below the center of the bolting silk baffle net (1) and is used for supporting the bolting silk baffle net (1) so as to increase the primary filtering area.
Drainage sponge (3): the filter tank (2) is filled with a plurality of layers of water filtering sponge (3) which are combined together to play a role in filtering the water body, so that particles in the water body are reduced from entering the microalgae amplification device.
Water baffle: is divided into a water baffle I (4) and a water baffle II (22). The water baffle I (4) is positioned at one side close to the water collecting port (20), the top end and the side edge of the water baffle I (4) are fixedly connected to the inner surface of the water filtering tank (2), and the bottom end of the water baffle I (4) is a distance away from the bottom end of the water filtering tank (2); the water baffle II (22) is located on one side close to the water storage tank (5), the bottom and the side of the water baffle II (22) are fixedly connected to the inner surface of the water filtering tank (2), and the top end of the water baffle II (22) is away from the top end of the water filtering tank (2) by a distance. The setting of breakwater I (4) and breakwater II (22) makes the sea water of getting into filter tank (2) from water collecting port (20), along breakwater I (4) bottom, breakwater II (22) top detouring in straining sponge (3), has increased sea water filterable journey, has improved the drainage performance of filter tank (2).
The water storage tank (5): the bottom of the water filtering tank (2) is used for temporarily storing the seawater filtered by the water filtering tank (2), and natural filtering of the seawater can be realized by the natural pressure of the seawater.
Outlet pipe (19): a short and thick pipeline is arranged at the water outlet of the water filtering tank (2) and is used for communicating the water filtering tank (2) with the water storage tank (5).
(2) Water lifting device
Lifting pump (11): the device is fixedly arranged on the outer wall of the reactor box body (17), and is used for conveying filtered seawater of the seawater in-situ filtering device and nutrient salt mother liquor of the nutrient salt supply device to the microalgae amplification device. A power switch (not shown) and a flow rate switch (24) for controlling the flow rate of the water body are arranged on the lifting pump (11).
The lower water lifting pipe (7): the top end of the water storage tank is connected with a water lifting pump (11), and the lower end of the water storage tank is inserted into the water storage tank (5).
Water lifting protection head (6): the inflatable air stone structure is arranged at the bottom end of the lower water lifting pipe (7) to protect the lower water lifting pipe (7) and prevent particulate matters from entering the microalgae amplification device.
Lifting water pipe (13): the bottom end of the micro algae amplification device is connected with a water lifting pump (11), and the top end of the micro algae amplification device is inserted into a reactor box body (17) of the micro algae amplification device to convey filtered seawater and nutrient salt mother liquor.
(3) Nutrient salt supply device
Mother liquor bottle (8): the opaque shading material is used for storing nutrient salt mother liquor required by algae cultivation.
Mother liquor extraction tube (9): is used for communicating the mother liquor bottle (8) with the lower water lifting pipe (7).
Control valve (10): and a control valve (10) which is arranged on the mother liquor extraction pipe (9) and is used for controlling the flow rate of the nutrient salt mother liquor through the opening/closing state of the control valve (10) (the minimum flow rate is zero, and the maximum flow rate is the full-open state of the mother liquor extraction pipe (9)).
The nutrient salt supply device can be hung on the water lifting device and then integrally placed on the microalgae amplification device for fixation.
(4) Microalgae amplification device
Reactor tank (17): a reactor for algae amplification culture is made of transparent glass plates, ensures functions of transparency, light absorption and the like, the top end of an upper water lifting pipe (13) stretches into a reactor box body (17), and filtered seawater and nutrient salt mother liquor flow into the reactor box body (17) from a top end water outlet (14) of the upper water lifting pipe (13).
Algae liquid spillover (16): the algae liquid overflow outlet (16) is arranged on the side wall of the reactor box and is positioned at one side far away from the water outlet (14) of the upper water lifting pipe (13), and the height of the algae liquid overflow outlet (16) is lower than that of the water outlet (14) of the upper water lifting pipe (13), so that after the water level in the reactor box body (17) naturally rises, the cultivated algae is discharged through the algae liquid overflow outlet (16).
Algae removal pipe (23): the high-density algae liquid is arranged at the algae liquid overflow port (16) and is convenient to discharge and directly enters the culture pond through the algae discharging pipe (23).
Mixing baffle (12): the device is arranged in a reactor box body (17), is arranged at 1/10 of one side of the box body and is close to a water outlet (14) of an upper water lifting pipe (13), the bottom end of a mixing partition plate (12) is at a certain height from the bottom of the reactor box body (17), the interior of the reactor box body (17) is divided into two parts, filtered seawater and nutrient salt mother liquor are pumped into the reactor box body (17) through the water outlet (14) of the upper water lifting pipe (13) under the action of a water lifting pump (11), and an upward flow is formed in the reactor box body (17) under the action of the mixing partition plate (12), so that the filtered water and the algae-phase water are uniformly mixed.
Reactor upper cover (15): the reactor is positioned at the top end of the reactor box body (17) and can be freely opened and closed, so that the cleaning and other operations in the reactor box body (17) are convenient.
Lighting device (not shown in the figures): the device is arranged on the lower surface of the upper cover (15) of the reactor, provides a light source for growth and propagation of algae liquid in the box body (17) of the reactor, is convenient for night or cloudy day time operation, and increases the capability of enlarging microalgae expansion cultivation.
Nano-gas tube (18): the gas supply device is paved at the bottom of the reactor box body (17) to ensure the gas supply in the algae cultivation.
The components of the microalgae in-situ expansion culture system are detachable, namely, the components forming the system can be transported independently and then assembled. The invention can be assembled into a seawater in-situ filtering device, a water lifting device, a nutrient salt supply device and a microalgae amplification device in a split mode, and then the 4 devices are installed together to form a complete microalgae in-situ amplification system.
The microalgae in-situ expansion culture system is placed beside a dyke of a shellfish culture pond and is properly fixed. The microalgae in-situ expansion culture system can be used as an independent unit for monomer arrangement, and can also be used for arranging a plurality of groups of microalgae in-situ expansion culture systems at the same time. The size of the microalgae in-situ expansion culture system (such as the size of a water filtering tank, a water storage tank, a mother liquor bottle, a reactor box body and the like) can be properly adjusted, so that the distribution quantity can be freely selected according to the concentration of baits in a water body, the shellfish culture density and the like.
Example 2
The application method of the microalgae in-situ expansion culture system for the shellfish culture pond mainly comprises two parts of experimental preparation work and specific application of the microalgae in-situ expansion culture system.
Experiment preparation:
(1) Preparing nutrient salt mother liquor: according to the difference of the microalgae to be cultivated, preparing nutrient salt mother liquor required by the spread cultivation of the microalgae seeds in a laboratory in advance, and then placing the mother liquor in a mother liquor bottle for standby.
(2) Expanding cultivation of algae: under the condition of a plastic barrel or a laboratory, the algae seeds are pre-spread and cultivated, then the algae seeds are transferred into a reactor box body, and the initial algae seed liquid level is properly adjusted according to the concentration of the spread and cultivated seeds, and the initial algae seed liquid level is preferably more than 1/5 of the volume of the reactor box body.
(3) And (3) system assembly: the components of the microalgae in-situ expansion culture system of example 1 are assembled into a whole according to a connection relationship.
The specific application of the microalgae in-situ expansion culture system comprises the following steps:
(1) The seawater in-situ filtering device is placed at the bottom of the shellfish culture pond, and after the seawater is filtered for the first time through a bolting silk blocking net (1) to block large-scale impurities, the seawater enters the water filtering tank from the water collecting port, and after the seawater is filtered for the second time along the bottom end of the water baffle I and the top end of the water baffle II in the water filtering sponge, the seawater flows into the water storage tank for standby.
(2) And starting a power switch of the water lifting pump, opening a control valve on the mother liquor extraction pipe, and pumping seawater in the water storage tank and nutrient salt mother liquor in the mother liquor bottle into the reactor box body.
(3) According to weather conditions and the conditions of microalgae in the reactor box body, a flow rate switch on a water lifting pump is regulated to control the water inflow into the reactor box body; the lighting device can be selectively turned on at night or in cloudy days to increase the light source required for microalgae propagation.
(4) Along with continuous injection of seawater and amplification of algae, when the liquid level exceeds an algae liquid overflow port, the amplified algae liquid is injected into a shellfish culture pond through an algae discharging pipe (23) to be fed to shellfish.
Through the steps, a circulating system for in-situ extraction of seawater in the shellfish culture pond, auxiliary injection of nutrient solution, high-order microalgae cultivation expansion and continuous algae solution supply is realized, and continuous supply of bait organisms is increased.
While the invention has been described in detail in the foregoing general description and specific examples, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Claims (4)

1. A method for in-situ culturing microalgae by using a microalgae in-situ expansion culture system, which is characterized by comprising the following steps:
1) According to the difference of the microalgae species to be cultivated, preparing a culture solution required by the expansion cultivation of the microalgae species in advance, and placing the culture solution in a mother liquor bottle for standby;
2) The algae seeds are spread in advance and then transferred into a reactor box body,
3) The seawater in-situ filtering device is placed at the bottom of the aquaculture pond, and after the seawater is filtered for the first time by the bolting silk blocking net to block large-scale impurities by means of the natural pressure of the seawater, the seawater enters the water filtering tank from the water collecting port, and flows into the water storage tank for standby after bypassing and filtering along the bottom end and the top end of the water baffle I and the water baffle II in the water filtering sponge;
4) Starting a power switch of the water lifting pump, opening a control valve on a mother liquor extraction pipe, pumping seawater in a water storage tank and nutrient salt mother liquor in a mother liquor bottle into a reactor box, and injecting amplified algae liquid into a shellfish culture pond through an algae discharging pipe to feed shellfish when the liquid level exceeds an algae liquid overflow port along with continuous injection of seawater and amplification of algae;
the microalgae in-situ expansion culture system comprises a water lifting device, a nutrient salt supply device, a microalgae expansion device and a seawater in-situ filtering device arranged in the culture pond; the seawater in-situ filtering device comprises a water filtering tank, a water outlet pipeline and a water storage tank, wherein the water filtering tank is filled with water filtering sponge, a water collecting port is formed in one side of the top end of the water filtering tank, and the opposite side of the bottom end of the water filtering tank is communicated with the water storage tank through the water outlet pipeline; the water lifting device comprises a lower water lifting pipe, a water lifting pump and an upper water lifting pipe which are connected in sequence, and the lower end of the lower water lifting pipe is inserted into the water storage tank; the nutrient salt supply device comprises a mother liquor extraction pipe and a light-tight mother liquor bottle filled with nutrient salt mother liquor, and the mother liquor bottle is communicated with the lower water lifting pipe through the mother liquor extraction pipe; the microalgae amplification device comprises a microalgae discharge pipe and a transparent reactor box body for microalgae culture, wherein the top end of the upper lifting pipe stretches into the reactor box body, and the side wall of the reactor box is provided with a microalgae liquid overflow port for connecting the microalgae discharge pipe;
a bolting silk blocking net is fixedly connected above the water collecting port; a supporting rod for supporting the bolting silk baffle net is arranged below the bolting silk baffle net;
the seawater in-situ filtering device also comprises a water baffle I and a water baffle II; the water baffle I is positioned at one side close to the water collecting port, the top end and the side edge of the water baffle I are fixedly connected to the inner surface of the water filtering tank, and the bottom end of the water baffle I is not contacted with the bottom end of the water filtering tank; the water baffle II is positioned at one side close to the water storage tank, the bottom end and the side edge of the water baffle II are fixedly connected to the inner surface of the water filtering tank, and the top end of the water baffle II is not contacted with the top end of the water tank;
a control valve for controlling the flow rate of the nutrient salt mother solution is arranged on the mother solution extraction pipe;
the algae liquid overflow outlet is positioned at one side far away from the water outlet of the upper water lifting pipe, and the height of the algae liquid overflow outlet is lower than that of the water outlet of the upper water lifting pipe; the microalgae amplification device further comprises a mixing baffle plate, wherein the mixing baffle plate is arranged in the reactor box body and divides the reactor box body into two parts, the bottom end of the mixing baffle plate is not contacted with the bottom of the reactor box body, and the mixing baffle plate is close to one side of a water outlet of the water lifting pipe.
2. The method according to claim 1, wherein the bottom end of the lower water lifting pipe is provided with a water lifting protection head for water filtering made of gas stone; the water lifting pump is provided with a flow rate switch.
3. The method of claim 1, wherein the top end of the reactor housing is provided with a removable reactor top cover; the lower surface of the reactor upper cover is provided with illumination equipment for providing a light source for microalgae growth; the bottom in the reactor box body is provided with a nano-gas-filled tube.
4. The method according to claim 1, wherein in the step 2), the initial expansion of the algal species is performed, and the liquid level of the algal species is 1/5 of the height of the reactor tank.
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Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010064780A1 (en) * 2008-12-03 2010-06-10 인하대학교 산학협력단 Photobioreactor for a large-scale marine microalgal culture using half-transmitting film
CN102735828A (en) * 2012-06-21 2012-10-17 中国地质大学（武汉） Test system for determining primary productivity of river benthic algae
CN204022814U (en) * 2014-08-05 2014-12-17 中国水产科学研究院南海水产研究所 A kind of full-automatic algatron
CN204014769U (en) * 2014-07-30 2014-12-17 中国海洋大学 Device is supported temporarily in a kind of Mytilus galloprovincialis laboratory
CN104488788A (en) * 2014-12-22 2015-04-08 浙江海洋学院 Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas
CN204779561U (en) * 2015-06-25 2015-11-18 上海海洋大学 Sea area normal position green alga culture apparatus
CN105441313A (en) * 2015-12-28 2016-03-30 海南大学 Hot-area microalgae culture system
CN106665324A (en) * 2016-12-15 2017-05-17 天津语山科技有限公司 Mechatronic cultivation experiment device
CN106993572A (en) * 2017-04-07 2017-08-01 苑春亭 A kind of sea pond ecological efficient cultural method
CN206565020U (en) * 2017-03-14 2017-10-20 中国水产科学研究院黄海水产研究所 Tangleweed original position experimental simulation system
CN107751085A (en) * 2017-09-21 2018-03-06 浙江海洋大学 A kind of circulating seawer cultivating system based on microalgae membrane bioreactor
CN108949576A (en) * 2018-09-05 2018-12-07 大连理工大学 A method of microalgae being cultivated in floatation type bioreactor using concentrated seawater
CN109169439A (en) * 2018-09-05 2019-01-11 浙江万里学院 A kind of level stream shellfish culture system using prawn culturing tail water
CN109287534A (en) * 2018-11-22 2019-02-01 宁波大学 A kind of marine shellfish cultivating pool microalgae group manual intervention system and application method
CN109355195A (en) * 2018-11-26 2019-02-19 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond
CN109769685A (en) * 2019-02-03 2019-05-21 福安市冠富农业科技有限公司 A kind of culture zone scene spreads cultivation algae method
CN208924990U (en) * 2018-09-10 2019-06-04 三门县海洋与渔业局 A kind of level stream recirculation system
CN208964634U (en) * 2018-06-19 2019-06-11 深圳文科园林股份有限公司 It is a kind of for river and the microalgae device of lake in-situ immobilization
CN210438740U (en) * 2019-08-08 2020-05-01 浙江万里学院 Microalgae in-situ expanding culture system for shellfish culture pond

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006282946A1 (en) * 2005-08-25 2007-03-01 Solix Biofuels, Inc. Method, apparatus and system for biodiesel production from algae
US20190216031A1 (en) * 2018-01-17 2019-07-18 Christopher James Hintz Algae Culturing

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010064780A1 (en) * 2008-12-03 2010-06-10 인하대학교 산학협력단 Photobioreactor for a large-scale marine microalgal culture using half-transmitting film
CN102735828A (en) * 2012-06-21 2012-10-17 中国地质大学（武汉） Test system for determining primary productivity of river benthic algae
CN204014769U (en) * 2014-07-30 2014-12-17 中国海洋大学 Device is supported temporarily in a kind of Mytilus galloprovincialis laboratory
CN204022814U (en) * 2014-08-05 2014-12-17 中国水产科学研究院南海水产研究所 A kind of full-automatic algatron
CN104488788A (en) * 2014-12-22 2015-04-08 浙江海洋学院 Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas
CN204779561U (en) * 2015-06-25 2015-11-18 上海海洋大学 Sea area normal position green alga culture apparatus
CN105441313A (en) * 2015-12-28 2016-03-30 海南大学 Hot-area microalgae culture system
CN106665324A (en) * 2016-12-15 2017-05-17 天津语山科技有限公司 Mechatronic cultivation experiment device
CN206565020U (en) * 2017-03-14 2017-10-20 中国水产科学研究院黄海水产研究所 Tangleweed original position experimental simulation system
CN106993572A (en) * 2017-04-07 2017-08-01 苑春亭 A kind of sea pond ecological efficient cultural method
CN107751085A (en) * 2017-09-21 2018-03-06 浙江海洋大学 A kind of circulating seawer cultivating system based on microalgae membrane bioreactor
CN208964634U (en) * 2018-06-19 2019-06-11 深圳文科园林股份有限公司 It is a kind of for river and the microalgae device of lake in-situ immobilization
CN108949576A (en) * 2018-09-05 2018-12-07 大连理工大学 A method of microalgae being cultivated in floatation type bioreactor using concentrated seawater
CN109169439A (en) * 2018-09-05 2019-01-11 浙江万里学院 A kind of level stream shellfish culture system using prawn culturing tail water
CN208924990U (en) * 2018-09-10 2019-06-04 三门县海洋与渔业局 A kind of level stream recirculation system
CN109287534A (en) * 2018-11-22 2019-02-01 宁波大学 A kind of marine shellfish cultivating pool microalgae group manual intervention system and application method
CN109355195A (en) * 2018-11-26 2019-02-19 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond
CN109769685A (en) * 2019-02-03 2019-05-21 福安市冠富农业科技有限公司 A kind of culture zone scene spreads cultivation algae method
CN210438740U (en) * 2019-08-08 2020-05-01 浙江万里学院 Microalgae in-situ expanding culture system for shellfish culture pond

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