CN110419475A - A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method - Google Patents
A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method Download PDFInfo
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- CN110419475A CN110419475A CN201910707796.7A CN201910707796A CN110419475A CN 110419475 A CN110419475 A CN 110419475A CN 201910707796 A CN201910707796 A CN 201910707796A CN 110419475 A CN110419475 A CN 110419475A
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- 241000270708 Testudinidae Species 0.000 title claims abstract description 34
- 230000029052 metamorphosis Effects 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 26
- FZRBKIRIBLNOAM-UHFFFAOYSA-N (E,E)-2-propynyl 3,7,11-trimethyl-2,4-dodecadienoate Chemical compound CC(C)CCCC(C)CC=CC(C)=CC(=O)OCC#C FZRBKIRIBLNOAM-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 229930001540 kinoprene Natural products 0.000 title claims abstract description 23
- 210000004907 gland Anatomy 0.000 title claims description 10
- 230000001418 larval effect Effects 0.000 title claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 63
- 239000003471 mutagenic agent Substances 0.000 claims abstract description 40
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
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- 238000002360 preparation method Methods 0.000 claims description 9
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- 230000000694 effects Effects 0.000 abstract description 7
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- 238000011161 development Methods 0.000 description 10
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- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000351387 Amphibalanus amphitrite Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241000345998 Calamus manan Species 0.000 description 1
- 241000131317 Capitulum Species 0.000 description 1
- 241000131327 Capitulum mitella Species 0.000 description 1
- 240000007126 Citrus medica var. sarcodactylis Species 0.000 description 1
- 241001301450 Crocidium multicaule Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- QVJMXSGZTCGLHZ-VWADHSNXSA-N Juvenile hormone III Natural products O=C(OC)/C=C(\CC/C=C(\CC[C@H]1C(C)(C)O1)/C)/C QVJMXSGZTCGLHZ-VWADHSNXSA-N 0.000 description 1
- 241000836026 Lepadomorpha Species 0.000 description 1
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- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
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- QVJMXSGZTCGLHZ-HONBPKQLSA-N juvenile hormone III Chemical compound COC(=O)\C=C(/C)CC\C=C(/C)CC[C@H]1OC1(C)C QVJMXSGZTCGLHZ-HONBPKQLSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of kinoprene dripping method step flow-induction tortoise foot cypris-form larva settlement and metamorphosis methods.This method is first, in accordance with kinoprene: dimethyl sulfoxide is the bulking value ratio hydrotropy of 1:45~60(w/v), and ethyl alcohol is added to be configured to the mutagens containing 5~8mg/mL of kinoprene.After the pond step culture pond A, the pond B fill clean seawater, cypris-form larva is moved into the pond A of step culture pond, in the seawater of the pond B and continues micro- inflation.Mutagens drop tube is opened later, and mutagens are continuously added dropwise to the pond A.The intake pump of starting step culture pond is filled the water, and continuous water injection time is 4-6 days.After 4-6 days, stops water filling and be added dropwise, take out cypris-form larva attachment base respectively, young body cultivation stages can be transferred to.Operation of the present invention is simple, less energy consumption, at low cost, and effect is stablized, and inducing effect is significant, and distortion ratio is a kind of good method for inducing tortoise foot cypris-form larva settlement and metamorphosis up to 80% or more.
Description
Technical field
The present invention relates to the technical fields of aquaculture, and in particular to a kind of kinoprene (CAS:42588-37-4) dropwise addition
Method step flow-induction tortoise foot cypris-form larva settlement and metamorphosis method
Background technique
Tortoise foot (Capitulum mitella Linnaeus) is a kind of sea with higher economic value and development prospect
Water breed variety is under the jurisdiction of Arthropoda, crust subphylum, cirrus infraclass, encloses chest catalogue, have handle mesh, armour Lepadomorpha, refer to tender tea leaves
Lotus section (Pollicipidae) tortoise belongs to (Capitulum) enough.Tortoise is commonly called as penholder, fingered citron, dog pawl spiral shell etc. enough, and general perch is in wave
In the rock crevice of the unobstructed middle and high tidal zone of severe impact water flow, be common in the distribution of all living creatures's tufted or with squama large bamboo hat with a conical crown and broad brim barnacle, the huge rattan of thorn
The associations such as pot are adhered to rock or other body surfaces.Due to the restriction by habitat, tortoise foot population scale is smaller and big
Small individual mixes, and the young is often attached to adult shank；Cluster individual is dug out when fishing, the children of a large amount of not up to edible specifications
Body is also pulled away, and destroys Stock resoures greatly；Sled the excavations behavior such as pounds and is also easily destroyed rock reef habitat, while on rock reef
Operation for the person of fishing also great risk.For reasonable development and protection tortoise foot resource, it is necessary to carry out people enough to tortoise
Work nursery and aquaculture research, fish wild resource in this way, can greatly reduce, control the excessive exploitation to environment, thus
The marine product resource and coastal ecology environment of effective protection Fujian Province.
Another category of Zhi Ming lotus section refers to that tender tea leaves lotus category (Pollicipe) has very big economic value.In Spain, Portugal
P.polymerus and P.pollicipes are more regarded as delicious food, the fishery resources quilt as a kind of huge economic value
High level of development utilizes, and has wide business demand and the very high market price, the price of per kilogram may be up to 200 Europe at present
Member.Due to excessive development and utilization, for the needs of protection, the states such as Spain limit its yield.
The history of life of cirrus class is typically passed through 6 phase nauplius, the cypris-form larva not ingested, fixation for seeking life of swimming
Young body and adult several stages of development.Cypris-form larva is an important stage in cirrus class growth course, unique to act on
It is that selection is suitable for the matrix of attachment and completes metamorphosis.During cypris-form larva settlement and metamorphosis, the swimmeret of cypris-form larva is quick
Stroke, is walked with " paces " of regularity in stromal surface, is detected attaching substratum by antennule, is secreted interim colloid and formed
Footprint；If matrix condition is suitable for, cypris-form larva if, secretes permanent colloid attachment, and then abnormal is young body, and individual enters fixed
Life stage.During settlement and metamorphosis, morphosis and life habit have occurred acutely the cypris-form larva that cirrus class is swum
Variation.If cypris-form larva cannot complete settlement and metamorphosis, with exhausting for nutriment, it is eventually striking to death.Stockless cirrus class
Barnacle is most important marine fouling organism, and foreign countries are with line barnacle (Amphibalanus amphitrite) for model organism pair
The settlement and metamorphosis mechanism of barnacle study for many years, it would be desirable to develop and interfere the efficient, green antifouling of its settlement and metamorphosis
Agent.Economic Pedunculate Cirripedia (referring to that tender tea leaves lotus belongs to and tortoise belongs to enough) belongs to different mesh from stockless cirrus class barnacle, their form
There is very big difference with life style.The adhesive for having handle class tortoise to secrete enough is anchored in matrix by means of the base portion of handle, nothing
The adhesive of handle class barnacle secretion is then anchored in matrix by entire chassis.Stockless class barnacle cypris-form larva under artificial condition
It is easy to settlement and metamorphosis, and has requirement of the handle class (tortoise belongs to enough and refer to tender tea leaves lotus category) to ecology special, the cypris-form larva under artificial condition
It is difficult to complete settlement and metamorphosis.For economic Pedunculate Cirripedia, it is artificial breeding that cypris-form larva completes settlement and metamorphosis indoors
It is crucial；To being stained for stockless cirrus class, interfering its settlement and metamorphosis is the key that prevent and kill off.
Although current laboratory is realized the artificial incubation from tortoise foot ovum, the culture of naupiar larva, can finally be trained on a large scale
Cypris-form larva is raised, but to realize the artificial breeding of tortoise foot, it is necessary to break through and induce the attachment of tortoise foot cypris-form larva under artificial condition
Abnormal problem.Settlement and metamorphosis under tortoise foot cypris-form larva artificial condition is the necessary process for completing its history of life indoors,
It is the key problem for realizing tortoise foot artificial breeding, it is therefore desirable to which tortoise foot cypris-form larva settlement and metamorphosis can effectively be induced by filtering out
Method or preparation.
Juvenile hormone and the like is most important a kind of insect hormone, has regulation insect growth, development, metamorphosis and life
The physiological function grown etc., can equally regulate and control the crustacean growth and development such as shrimp crab.Although in recent years to the effect molecule of juvenile hormone
Mechanism has conducted extensive research, but thoroughly discloses the mechanism of action of juvenile hormone far away.Due to juvenile hormone and the like type
It is various, and there are a variety of physiological regulation functions；Insect and crustacean wide variety, and same species different stage of development,
Concentration, opportunity of effect of drug etc. can all influence the effect of hormone.Therefore, a certain hormone is directed to the effect of a certain species often
It is different.Chinese patent (patent No. 201510183853.8) discloses " a kind of to induce tortoise pure gold star using juvenile hormone-III
The method of larva settlement and metamorphosis ".On the basis of working herein, it is main for being filtered out again with juvenoid kinoprene
The mutagenesis preparation of composition.
Summary of the invention
According to our result of study, it is attached that the present invention using kinoprene as main function composition is configured to tortoise foot cypris-form larva
Abnormal special preparation；Said preparation application method is simple, can reach 55% or more using the distortion ratio of rear cypris-form larva, obtains
Young body can normal growth and development become adult.
In order to achieve the above objectives, the technical scheme adopted by the invention is as follows:
1. the preparation of the mutagens of tortoise foot cypris-form larva settlement and metamorphosis:
According to kinoprene: dimethyl sulfoxide is the bulking value ratio hydrotropy of 1:45~60 (w/v), and ethyl alcohol is added to be configured to
Mutagens containing 5~8mg/mL of kinoprene, cryo-conservation under the conditions of 4 DEG C.
2. the cypris-form larva for collecting culture is moved into ladder first after the pond step culture pond A, the pond B fill clean seawater
In the grade pond A of culture pond, the pond B seawater.
3, the pond A, the pond B seawater carry out persistently micro- inflation, disperse cypris-form larva freely.
4, micro- inflation after ten minutes, opens mutagens drop tube, and mutagens, the speed that mutagens are added dropwise continuously is added dropwise to the pond A
Degree is dripped with the pond A, the watermeter of B water body total volume, every Liter Per Minute 6.
5. being added dropwise after twenty minutes, start the intake pump of step culture pond, water injection pipe uninterruptedly fills the water the pond A, and 24 is small
When water injection rate be the pond A, B water body total volume 1/3.Continuous water injection time is 4-6 days.The clean sea water of injection, after mixing
The pond C that step culture pond is flowed into via the pond A, the pond B is gone out to cultivate tail water after C water is full by discharge pipe batch.The setting in the pond C,
The residence time of induction seawater is extended, while the unattached cypris-form larva in the pond A, the pond B can further be induced attachment, effectively
Prevent the loss of cypris-form larva living body.
6, after 4-6 days, stop water filling and be added dropwise, take out cypris-form larva attachment base respectively, young body cultivation stages can be transferred to.
It can be found that the pond A, the pond B cypris-form larva settlement and metamorphosis amount are maximum from cypris-form larva attachment base is taken out, and the pond C belongs to A
The tail water in pond, the pond B still has the settlement and metamorphosis body of part.
The clean sea water: water temperature is 23-28 DEG C, salinity 25-35, pH 7.8-8.1.
Step culture pond of the present invention is by step pond, air system, seawater injection pipe and mutagens drop tube structure
At, wherein step pond is arranged successively from high to low by the height of the pond A, the pond B, the pond C peace water level, between the pond A, the pond B, the pond B,
Between the pond C, it is connected respectively by the pond A overflow pipe, the pond B overflow pipe；The seawater Chu Manhou in the pond C, tail water are discharged by draft tube；Sea
Water injection pipe is opened on the pond A, and mutagens drop tube is located at the top in the pond A.
The air system is made of air compressor machine, appendix and quick-fried vapour disk and is connected with this, and appendix, which is layed in, to be located at
The pond A, the pond B, the pond C bottom, gas explosion disk be arranged in after being connect with air hose the pond A, the pond B, the pond C bottom center at.
The valvular dropwise addition control of the road 5-10 band is arranged side by side in mutagens drop tube outlet end in the mutagens drop tube
Head processed.
The kinoprene, is purchased in Reagent Company.
46 days tracking tests of batch have been carried out using the method for the present invention, every batch of equivalent puts into 10000 cypris-form larvas,
Every batch of tortoise pedal gland Jie's larval metamorphosis is recorded, and to develop into young body distortion ratio data as follows:
As can be seen from the above table, the distortion ratio that 4 batch of tracking test group of the present invention cultivates young body is all higher than 80%, average
Can reach 82.5%, the young body of acquisition can normal growth and development become adult.
Operation of the present invention is simple, less energy consumption, at low cost, effect stablize, inducing effect is significant, distortion ratio up to 80% with
On.Method presents the suitable concentration of formulation application, processing time and induction starting times, are that a kind of induction tortoise foot cypris-form larva is attached
Abnormal good method.
Detailed description of the invention
Fig. 1 is step culture pond structural schematic diagram described in the utility model.
Fig. 2 is step culture pond mutagens drop tube structural schematic diagram described in the utility model.
Specific embodiment
The utility model is continuously induced in multiple batches of tortoise foot cypris-form larva, reaches good effect.With
The present invention is described further for lower way of example.
In Fig. 1,1 is mutagens drop tube；2 be the pond A in step pond；3 be the pond A overflow pipe；4 be in step pond
The pond B；5 be the pond B overflow pipe；6 be the pond C in step pond；7 be the draft tube on the side wall of the pond C；8 be the gas explosion in air system
Disk；9 be appendix；10 be seawater injection pipe；11 be air compressor machine.The pond A (2), the pond B (4), the pond C (6) in step water level worship it is high to Low
Arrangement.The pond A overflow pipe (3) is connected to the pond A (2) and the pond B (4)；The pond B overflow pipe (5) is connected to the pond B (4) and the pond C (6).
In Fig. 2,1 is mutagens drop tube；12 be that the road 5-10 is arranged side by side with valve in mutagens drop tube (1) outlet end
Dropwise addition control head.
Embodiment 1
1. the preparation of the mutagens of tortoise foot cypris-form larva settlement and metamorphosis:
According to kinoprene: dimethyl sulfoxide is the bulking value ratio hydrotropy of 1:45~60 (w/v), and ethyl alcohol is added to be configured to
Mutagens containing kinoprene 8mg/mL, cryo-conservation under the conditions of 4 DEG C.
2. step culture pond:
Step described in the present embodiment cultivates pool structure as shown in technical solution.Wherein:
The size of the pond A (2) in the step pond are as follows: 1000mm (length) × 1500mm (width) × 1000mm (height), note
Water height 800mm.
The size of the pond B (4) in the step pond are as follows: 1200mm (length) × 1500mm (width) × 900mm (height), water filling
Height 700mm.
The size of the pond C (6) in the step pond are as follows: 800mm (length) × 1500mm (width) × 800mm (height), water filling
Height 600mm.After the seawater in the pond C (6) is full of, tail water is discharged by draft tube (7).
3. cultivating:
1, first after the pond step culture pond A (2), the pond B (4) fill clean seawater, 5600 glands of culture will be collected
Jie larva moves into the pond A (2) of step culture pond, in the pond B (4) seawater.
2, persistently micro- inflation is carried out to the pond A (2), the pond B (4) seawater, disperses cypris-form larva freely.
3, micro- inflation after ten minutes, is opened mutagens drop tube (1), and mutagens, mutagens drop is continuously added dropwise to the pond A (2)
The speed added 6 drop per minute, the dropwise addition on 10 tunnel Gong Kai control head (1).
4. being added dropwise after twenty minutes, start the intake pump of step culture pond, water injection pipe (10) uninterruptedly infuses the pond A (2)
Water, control 24 hours 0,5 cubic metres of water injection rate.Continuous water injection time is 5 days.The clean sea water of injection, through by the pond A after mixing
(2), the pond B (4) flow into the pond C (6) of step culture pond, after the pond C (6) water is full, is discharged by draft tube (7) and cultivates tail water.
5, after 5 days, stop water filling and be added dropwise, take out cypris-form larva attachment base respectively, young body cultivation stages can be transferred to.
6, it takes out cypris-form larva attachment base to be counted, the young body of metamorphosis in the pond A (2), the pond B (4) and the pond C (6) is respectively as follows:
1994,2478,189, be 4661 after merging, and total distortion ratio is 83.23%.
Clean sea water used in the present embodiment: water temperature is 23-28 DEG C, salinity 25-35, pH 7.8-8.1.
Embodiment 2
1. the preparation of the mutagens of tortoise foot cypris-form larva settlement and metamorphosis:
According to kinoprene: dimethyl sulfoxide be 1:45 (w/v) bulking value ratio hydrotropy, add ethyl alcohol be configured to containing
The mutagens of kinoprene 8mg/mL, cryo-conservation under the conditions of 4 DEG C.
2. step cultivates pool structure:
Step described in the present embodiment cultivates pool structure as shown in technical solution.Wherein:
The size of the pond A (2) in the step pond are as follows: 1 meter of (length) × 1.5 meter (width) × 1 meter (height), water filling height
800mm。
The size of the pond B (4) in the step pond are as follows: 1.2 meters of (length) × 1.5 meter (width) × 0.9 meter (height), water filling are high
0.7 meter of degree.
The size of the pond C (6) in the step pond are as follows: 0.8 meter of (length) × 1.5 meter (width) × 0.8 meter (height), water filling are high
0.6 meter of degree.After the seawater in the pond C (6) is full of, tail water is discharged by draft tube (7).
3. cultivating:
1) first after the pond step culture pond A (2), the pond B (4) fill clean seawater, 5600 glands of culture will be collected
Jie larva moves into the pond A (2) of step culture pond, in the pond B (4) seawater.
2) persistently micro- inflation is carried out to the pond A (2), the pond B (4) seawater, disperses cypris-form larva freely.
3) micro- inflation after ten minutes, is opened mutagens drop tube (1), and mutagens, mutagens drop is continuously added dropwise to the pond A (2)
The speed added 4 drop per minute, the dropwise addition on 10 tunnel Gong Kai control head (12), are persistently added dropwise 150 minutes.
4) after being added dropwise 150 minutes, start the intake pump of step culture pond, water injection pipe (10) uninterruptedly infuses the pond A (2)
Water, control 24 hours 0.5 cubic metre of water injection rate.Continuous water injection time is 5 days.The clean sea water of injection, through by the pond A after mixing
(2), the pond B (4) flow into the pond C (6) of step culture pond, after the pond C (6) water is full, is discharged by draft tube (7) and cultivates tail water.
5) after 5 days, stop water filling and be added dropwise, take out cypris-form larva attachment base respectively, young body cultivation stages can be transferred to.
6) it takes out cypris-form larva attachment base to be counted, the young body of metamorphosis in the pond A (2), the pond B (4) and the pond C (6) is respectively as follows:
1994,2478,189, be 4661 after merging, and total distortion ratio is 83.23%.
Clean sea water used in the present embodiment: water temperature is 23 DEG C, salinity 35, pH 7.8.
Embodiment 3
1. the preparation of the mutagens of tortoise foot cypris-form larva settlement and metamorphosis:
According to kinoprene: dimethyl sulfoxide be 1:45 (w/v) bulking value ratio hydrotropy, add ethyl alcohol be configured to containing
The mutagens of kinoprene 8mg/mL, cryo-conservation under the conditions of 4 DEG C.
2. step cultivates pool structure:
Step described in the present embodiment cultivates pool structure as shown in technical solution.Wherein:
The size of the pond A (2) in the step pond are as follows: 1 meter of (length) × 1 meter (width) × 1 meter (height), water filling height 0.8
Rice, water body volume are 1 cubic metre.
The size of the pond B (4) in the step pond are as follows: 1 meter of (length) × 1 meter (width) × 0.9 meter (height), water filling height
0.7 meter.
The size of the pond C (6) in the step pond are as follows: 0.8 meter of (length) × 1 meter (width) × 0.8 meter (height), water filling height
0.6 meter.After the seawater in the pond C (6) is full of, tail water is discharged by draft tube (7).
3. cultivating:
1) first after the pond step culture pond A (2), the pond B (4) fill clean seawater, 5600 glands of culture will be collected
Jie larva moves into the pond A (2) of step culture pond, in the pond B (4) seawater.
2) persistently micro- inflation is carried out to the pond A (2), the pond B (4) seawater, disperses cypris-form larva freely.
3) micro- inflation after ten minutes, is opened mutagens drop tube (1), and mutagens, mutagens drop is continuously added dropwise to the pond A (2)
The speed added 5 drop per minute, the dropwise addition on 10 tunnel Gong Kai control head (12), are persistently added dropwise 20 minutes.
4) it is added dropwise after twenty minutes, starts the intake pump of step culture pond, water injection pipe (10) uninterruptedly infuses the pond A (2)
Water, control 24 hours 0.36 cubic metre of water injection rate.Continuous water injection time is 4 days.The clean sea water of injection, through by A after mixing
Pond (2), the pond B (4) flow into the pond C (6) of step culture pond, after the pond C (6) water is full, is discharged by draft tube (7) and cultivates tail water.
5) after 4 days, stop water filling and be added dropwise, take out cypris-form larva attachment base respectively, young body cultivation stages can be transferred to.
6) it takes out cypris-form larva attachment base to be counted, the young body of metamorphosis in the pond A (2), the pond B (4) and the pond C (6) is respectively as follows:
1994,2478,189, be 4661 after merging, and total distortion ratio is 83.23%.
Clean sea water used in the present embodiment: water temperature is 25 DEG C, salinity 30, pH8.1.
Claims (4)
1. a kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method, it is characterised in that:
1) preparation of the mutagens of tortoise foot cypris-form larva settlement and metamorphosis:
According to kinoprene: dimethyl sulfoxide be 1:45~60(w/v) bulking value ratio hydrotropy, add ethyl alcohol be configured to containing
The mutagens of 5~8mg/mL of kinoprene, cryo-conservation under the conditions of 4 DEG C；
2) first after the pond step culture pond A, the pond B fill clean seawater, the cypris-form larva for collecting culture is moved into step training
Educate the pond A in pond, in the seawater of the pond B；
3) persistently micro- inflation is carried out to the pond A, the pond B seawater, disperses cypris-form larva freely；
4) micro- inflation after ten minutes, opens mutagens drop tube, mutagens is continuously added dropwise to the pond A, the speed that mutagens are added dropwise is with A
Pond, B water body total volume watermeter, every Liter Per Minute 6 drips；
5) it is added dropwise after twenty minutes, starts the intake pump of step culture pond, water injection pipe uninterruptedly fills the water the pond A, infuses within 24 hours
Water be the pond A, B water body total volume 1/3.
2. a kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method according to claim 1,
It is characterized in that the step culture pond is made of step pond, air system, seawater injection pipe and mutagens drop tube,
Middle step pond by the pond A, the pond B, the pond C peace water level height be arranged successively from high to low, between the pond A, the pond B, the pond B, the pond C it
Between, it is connected respectively by the pond A overflow pipe, the pond B overflow pipe；The seawater Chu Manhou in the pond C, tail water are discharged by draft tube；Seawater note
Enter tube opening in the pond A, mutagens drop tube is located at the top in the pond A.
3. a kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method according to claim 2,
It is characterized in that the air system, is made of air compressor machine, appendix and quick-fried vapour disk and is connected with this, appendix is layed in position
In the pond A, the pond B, the pond C bottom, gas explosion disk be arranged in after being connect with air hose the pond A, the pond B, the pond C bottom center at.
4. a kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method according to claim 2,
It is characterized in that the mutagens drop tube, is arranged side by side the valvular dropwise addition of the road 5-10 band in mutagens drop tube outlet end
Control head.
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| CN111771806A (en) * | 2020-07-15 | 2020-10-16 | 福建师范大学 | Tortoise foot venus larva attachment metamorphosis device |
| CN113243341A (en) * | 2021-06-22 | 2021-08-13 | 中国人民解放军国防科技大学 | Barnacle larva breeding device, barnacle larva breeding method, antifouling test system, breeding-antifouling test system and antifouling test method |
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| WO2003037077A1 (en) * | 2001-11-02 | 2003-05-08 | The State Of Queensland, Through Its Departement Of Primary Industries | Method and apparatus for aquatic animal husbandry |
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| CN111771806B (en) * | 2020-07-15 | 2022-03-29 | 福建师范大学 | Tortoise foot venus larva attachment metamorphosis device |
| CN113243341A (en) * | 2021-06-22 | 2021-08-13 | 中国人民解放军国防科技大学 | Barnacle larva breeding device, barnacle larva breeding method, antifouling test system, breeding-antifouling test system and antifouling test method |
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