CN111771806A - Tortoise foot venus larva attachment metamorphosis device - Google Patents
Tortoise foot venus larva attachment metamorphosis device Download PDFInfo
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- CN111771806A CN111771806A CN202010678044.5A CN202010678044A CN111771806A CN 111771806 A CN111771806 A CN 111771806A CN 202010678044 A CN202010678044 A CN 202010678044A CN 111771806 A CN111771806 A CN 111771806A
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- 230000029052 metamorphosis Effects 0.000 title claims abstract description 49
- 241000270708 Testudinidae Species 0.000 title claims description 14
- YMHOBZXQZVXHBM-UHFFFAOYSA-N 2,5-dimethoxy-4-bromophenethylamine Chemical compound COC1=CC(CCN)=C(OC)C=C1Br YMHOBZXQZVXHBM-UHFFFAOYSA-N 0.000 title claims description 8
- 241000545067 Venus Species 0.000 title claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000007599 discharging Methods 0.000 claims abstract description 5
- 241000465412 Hemsleya amabilis Species 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 6
- 239000013535 sea water Substances 0.000 description 6
- 231100000611 venom Toxicity 0.000 description 5
- 241000218691 Cupressaceae Species 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000238588 Balanus Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 244000060234 Gmelina philippensis Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 125000003003 spiro group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 235000005781 Avena Nutrition 0.000 description 1
- 241000209761 Avena Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241001262815 Dactylogyrus Species 0.000 description 1
- 241000577951 Hydnum Species 0.000 description 1
- QVJMXSGZTCGLHZ-VWADHSNXSA-N Juvenile hormone III Natural products O=C(OC)/C=C(\CC/C=C(\CC[C@H]1C(C)(C)O1)/C)/C QVJMXSGZTCGLHZ-VWADHSNXSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000133262 Nauplius Species 0.000 description 1
- 241000221365 Tremellales Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- QVJMXSGZTCGLHZ-HONBPKQLSA-N juvenile hormone III Chemical compound COC(=O)\C=C(/C)CC\C=C(/C)CC[C@H]1OC1(C)C QVJMXSGZTCGLHZ-HONBPKQLSA-N 0.000 description 1
- 229930000772 juvenile hormones III Natural products 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides an adhesion metamorphosis device for the cyprids of Hemsleya amabilis, which is characterized in that the device comprises a top cover, an inner barrel and an outer barrel, wherein the shape of the top cover is semicircular; the inner barrel is formed by wrapping a plastic framework and a 200-mesh screen, eight attaching platforms for storing attaching bases are arranged at the 2/3-high position of the inner barrel, and a round hole is formed in the center of the bottom of the inner barrel, so that the outer barrel can be conveniently connected with a water pipe; the outer barrel is made of thickened PE materials, a fixed submersible pump is arranged in the center of the bottom of the outer barrel and connected with a PE water pipe to be connected with a round hole in the bottom of the inner barrel, a water inlet is formed in the outer side of the upper portion of the outer barrel, a water discharging pipe is arranged in the outer side of the bottom of the outer barrel, and an electromagnetic ball valve is.
Description
Technical Field
The invention relates to an aquaculture technology, in particular to an attachment metamorphosis device for cyprids.
Background
The Hedgehog hydnum is selected from Arthropoda, Crustacea, Tremellales, Tominaceae, Hepoda, and Hedgehog, also named as Shi\34576, fructus Citri Sarcodactylis Spiro, pen rack, dog paw Spiro, etc.. Is widely distributed in the ocean of India and Pacific, and only one of the Tortoise feet is in China, mainly distributed in coastal waters such as Zhejiang, Fujian, Taiwan and Guangdong, and is an animal in subtropical zone and intertidal zone of tropical zone. The tortoise foot is peculiar in shape, lives in the intertidal zone of a medium and high tide region with violent impact of sea waves, is attached to a rock gap by a handle part, and grows densely in a cluster shape. The turtle-foot handle muscle is edible, delicious in taste and high in nutritive value, is a high-quality food which is high in protein, low in fat, rich in mineral substances, has various physiological functions and health care effects, and has unique economic value and wide market prospect.
The tortoise feet in the market are all from wild collection, but the wild collection faces the problems of sharp reduction of wild resource amount, no guarantee of the damaged quality of the muscle of the handle, difficult collection, long market free period and the like, and the artificial breeding research is necessary for better developing and utilizing the tortoise foot resources. The life history of the tortoise foot usually passes through several development stages of 6-stage nauplius larva in nutrition-planktonic life, non-feeding cypress larva, sessile tortoise foot larva and adult, wherein the main task of the cypress larva is to find a proper substrate for adhesion and metamorphosis. The method is a very critical step, the morphological structure and the life habit of the cyprids of the tendrilled are changed violently in the attachment and metamorphosis process, and if the cyprids of the tendrilled cyprids cannot complete the attachment and metamorphosis, the cyprids of the tendrilled cyprids will die with the exhaustion of nutrient substances. Although the artificial incubation of tortoise-foot eggs, the culture of nauplii and the final large-scale culture of venomous larvae are realized in the laboratory at present, the metamorphosis process can be started only after the venomous larvae are attached, the axostoma larvae without stems are easy to be attached and metamorphosed under the artificial condition, the ecological requirements of stems (tortoise-foot and dactylogyrus) are special, and the ademetia is difficult to be attached and metamorphosed under the artificial condition.
The attachment metamorphosis of the cyprids of the tortoiseshell under artificial conditions is a necessary process for completing the life history indoors, and is also a core problem for realizing the artificial breeding of the tortoises. Chinese patent No. 201510183853.8 discloses a method for inducing settlement and metamorphosis of cypress larva with juvenile hormone-III, and Chinese patent No. 201510183885.8 discloses a method for inducing settlement and metamorphosis of cypress larva with gland. The metamorphosis process of the balanus cerasus larva is completed through induction, and the invention simulates the attachment metamorphosis habitat of the balanus cerasus larva under the natural condition and tries to completely simulate the process indoors to complete the attachment metamorphosis process.
Disclosure of Invention
The invention aims to provide a settlement and metamorphosis device for cyprids of mars glauca to solve the problems in the background technology. The device can provide a place required for settlement and metamorphosis for artificially cultured cyprids of avena guipodi, greatly improve the settlement and metamorphosis rate of the cyprids of cyprids.
In order to achieve the purpose, the invention provides the following technical scheme:
an adhesion metamorphosis device for Tortoise foot venus larva comprises a top cover, an inner barrel and an outer barrel. Wherein the top cover is semicircular in shape and black in appearance; the inner barrel is formed by wrapping a plastic framework and a 200-mesh screen, eight attaching platforms for storing attaching bases are arranged at the 2/3-high position of the inner barrel, and a round hole is formed in the center of the bottom of the inner barrel, so that the outer barrel can be conveniently connected with a water pipe; the outer barrel is made of thickened PE materials, a fixed submersible pump is arranged in the center of the bottom of the outer barrel and connected with a PE water pipe to be connected with a round hole in the bottom of the inner barrel, a water inlet is formed in the outer side of the upper portion of the outer barrel, a water discharging pipe is arranged in the outer side of the bottom of the outer barrel, and an electromagnetic ball valve is.
The invention has the beneficial effects that: the top cover is in a semicircular shape and plays a role in protecting larvae in the barrel, and the semicircular structure enables seawater and cyprids sprayed to the top to flow to the attachment platform along the inner wall after the water pump in the device is started; the appearance is black, the interior of the barrel is shaded, and the attachment and metamorphosis environment of the cyprids is simulated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic structural diagram of a settlement metamorphosis device for cyprids of marsupium glauca larvae, provided by the embodiment of the invention.
Fig. 2 is a cross-sectional view of a settlement metamorphosis device for cyprids of the star warrior of the present invention.
Fig. 3 is a schematic structural diagram of an inner barrel in the settlement metamorphosis device for the balanus cerasus larvae provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1-3, a device for adhering and metamorphosing Tortoise foot venus larva comprises a top cover 1, an inner barrel 3 and an outer barrel 2. Wherein the top cover 1 is semicircular in shape and black in appearance; the inner barrel 3 is formed by wrapping a plastic framework and a 200-mesh screen, eight attaching platforms 4 for storing attaching bases are arranged at the 2/3-high position of the inner barrel 3, and a round hole 5 is formed in the center of the bottom of the inner barrel, so that the outer barrel can be conveniently connected with a water pipe; the outer barrel 2 is made of thickened PE material, a fixed submersible pump 5 is arranged in the center of the bottom and connected with a PE water pipe to be connected with a round hole in the bottom of the inner barrel 3, a water inlet is arranged on the outer side of the upper part, a water discharge pipe 6 is arranged on the outer side of the bottom, and an electromagnetic ball valve is arranged on the upper part. When in use, the three parts are connected in sequence and then connected into a whole in a jogged mode, and the volume of the water body of the adhering metamorphosis device is 100L.
The top cover 1 is semicircular in shape and plays a role in protecting larvae in the barrel, and the semicircular structure enables seawater and cyprids sprayed to the top to flow to the attachment platform 4 along the inner wall after the water pump 5 in the device is started; the appearance is black, the interior of the barrel is shaded, and the attachment and metamorphosis environment of the cyprids is simulated.
The inner barrel is composed of a PE material framework and a 200-mesh screen, and 8 attaching platforms capable of storing attaching bases are arranged at the height of the inner barrel 2/3. The 200-mesh screen at the periphery and the bottom can prevent cyprids from dissipating to the outer barrel and discharging water, the weight and the cost of the inner barrel are greatly reduced on the basis of ensuring the structural integrity by combining the framework and the screen, and the inner barrel can be taken out of the whole inner barrel after attachment is completed or the attachment medium is taken out separately and moved to other culture barrels to culture larvae.
The outer barrel is made of thickened PE materials, a fixed water pump is arranged in the center of the bottom of the outer barrel, an upper water receiving pipe is connected with a round hole in the bottom of the inner barrel, a water inlet is formed in the outer side of the upper portion of the outer barrel, a water discharging pipe is arranged in the outer side of the bottom of the outer barrel, and an electromagnetic ball valve is arranged on the upper. The timing device is connected to the water pump power supply, the timing device is started every 5min for 10s, the water pump is used for ejecting water upwards from the central water pipe, on one hand, water flow carrying the cyprids is enabled to rush to the top cover, then the cyprids are scattered in the attaching base or return to the water body along the edge of the top cover, on the other hand, the water body is disturbed through the impulsive force of the water pump, the impact of sea waves on reef is simulated, and the cyprids in the water body are enabled to be continuously contacted with the attaching base along with the flowing of the water. After the bottom drain pipe is opened to an appointed gear, the water inlet speed of the water inlet is the same as the water outlet speed of the water outlet, the designed water inlet speed is 1L/h, the attachment metamorphosis and water changing feeding work can be completed only by controlling a power switch (or intelligently timing the switch), and labor is saved.
Because the cyprids generally live in the upper strata of water in a planktonic form, but do not exclude some cyprids can move about in interior bucket lower floor because rivers or self factor influence, consequently can place some adherance in interior bucket bottom, not only can provide the adherance matrix to middle and lower floor cyprids, improve the attachment rate, still can play certain collection guard action to not attaching the larva, prevent to lead to the larva to be difficult to move about to the upper strata because lower floor's drainage and accomplish to adhere to the metamorphosis, improve larva and adhere to the metamorphosis rate.
The adhesion metamorphosis device designed by the invention is compared with a PE cultivation barrel (manual water changing and bait casting) with a cover and the same adhesion base in a water body to perform a plurality of groups of comparative experiments of adhesion and growth, and the specific data are as follows:
example one: and 6, 20 days in 2019, and carrying out a jinxing larva settlement and metamorphosis experiment in a southern ocean research institute culture room of the university of Fujian teachers. The device and the culture barrel are placed in the same environment, the same attaching base is added, 100L of filtered seawater is added respectively, wherein the water temperature is 25 ℃, the salinity is 29, and the pH is 8.0. Culturing and collecting the cyprids of Venus macranthus, putting 2000 cyprids of Hymenopterus into the barrel, adjusting the opening and closing of the cyprids, and covering the barrel cover to start the experiment.
After 7 days, 729 cyprids finish attachment metamorphosis on average, the attachment metamorphosis rate reaches 36.5%, and the attachment metamorphosis rate of the breeding barrel is only 3.2%.
TABLE 1 comparison of settlement and metamorphosis of cyprids and venomous parvus
Description of the drawings: the counting mode adopts brush washing and microscope counting.
Example two: and carrying out a jinxing larva settlement metamorphosis experiment in a southern ocean research institute culture room of university of Fujian teachers at 8 and 29 months in 2019. The device and the culture barrel are placed in the same environment, the same attaching base is added, 100L of filtered seawater is added respectively, wherein the water temperature is 26 ℃, the salinity is 29.3, and the pH is 8.0. Culturing and collecting cyprids of Venus macranthus, putting 5000 cyprids of Hymenopterus into the barrel, adjusting the opening and closing of the cyprids, and covering the top barrel cover to start the experiment.
The device finishes attachment and metamorphosis of 1616 cyprids on average after 7 days, the attachment and metamorphosis rate reaches 37.3 percent, and the attachment and metamorphosis rate of the breeding barrel is only 4.3 percent.
TABLE 2 comparison of settlement and metamorphosis of cyprids and venomous parvus
Description of the drawings: the counting mode adopts brush washing and microscope counting.
Example three: and carrying out settlement and metamorphosis experiments on the cyprids in the culture room of southern ocean research institute of university of Fujian teachers in 2019, 10 months and 20 days. The device and the culture barrel are placed in the same environment, the same attaching base is added, 100L of filtered seawater is added respectively, wherein the water temperature is 24 ℃, the salinity is 29.0, and the pH is 7.9. Culturing and collecting cyprids of Venus macranthus, putting 15000 cyprids of Hymenophilus into the bucket, adjusting respective switches, and covering the top of the bucket to start the experiment.
The device finishes the attachment and metamorphosis of 5576 cyprids on average after 7 days, the attachment and metamorphosis rate reaches 40.5 percent, and the attachment and metamorphosis rate of the breeding barrel is only 2.5 percent.
TABLE 3 comparison of settlement and metamorphosis of cyprids and venomous parvus
Description of the drawings: the counting mode adopts brush washing and microscope counting.
On the basis of the settlement and metamorphosis comparison experiment, the settlement and metamorphosis rates of the cyprids with different biomass are counted, and data show that by adopting the device, the higher the biomass is, the higher the settlement and metamorphosis rate is, and the highest settlement and metamorphosis rate can be 16 times that of a common breeding barrel; in addition, after the cultivation and observation are continued for 5 weeks, the larva bodies of the tortoise feet are continuously cultivated in the device, and the dropping rate or the death rate of the larva bodies is far lower than that of a common cultivation barrel. When a large amount of fishes are cultivated, only 1-2 persons are needed to filter and cultivate the seawater and the baits by setting a timing switch; however, when the common cultivation barrel is used for cultivation, the manpower required to be paid is multiplied along with the increase of the cultivation quantity.
The device structural design is reasonable, and the interior bucket of breeding device, outer bucket and top cap firmly the gomphosis together, combine attachment system and electrical system. The inner barrel not only can be used as an attaching base attaching platform in attaching metamorphosis, but also can be integrally moved to a common breeding barrel to be directly used as a double-layer breeding frame, the surrounding filter screen can obviously improve death caused by the dropping of the tortoise foot due to mechanical force or water flow impact force, and the attaching base laid at the bottom layer can provide secondary attaching effect. The whole set of device can realize automatic management through an intelligent time control switch, can be used for large-scale production and cultivation of the tortoise-foot fries, and can greatly improve the cultivation rate of the tortoise-foot fries.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. An adhesion metamorphosis device for the cyprids of the tortoise foot venus is characterized in that the device comprises a top cover, an inner barrel and an outer barrel, wherein the shape of the top cover is semicircular; the inner barrel is formed by wrapping a plastic framework and a 200-mesh screen, eight attaching platforms for storing attaching bases are arranged at the 2/3-high position of the inner barrel, and a round hole is formed in the center of the bottom of the inner barrel, so that the outer barrel can be conveniently connected with a water pipe; the outer barrel is made of thickened PE materials, a fixed submersible pump is arranged in the center of the bottom of the outer barrel and connected with a PE water pipe to be connected with a round hole in the bottom of the inner barrel, a water inlet is formed in the outer side of the upper portion of the outer barrel, a water discharging pipe is arranged in the outer side of the bottom of the outer barrel, and an electromagnetic ball valve is.
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EP3372073A1 (en) * | 2017-03-09 | 2018-09-12 | Universidade de Évora | Device and process for fixation of larvae and growth of juveniles of the stalked barnacle pollicipes pollicipes |
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CN210352744U (en) * | 2019-08-01 | 2020-04-21 | 福建师范大学 | Light and medicine combined method induced tortoise-foot juvenile industrial artificial breeding pond |
CN210352704U (en) * | 2019-07-05 | 2020-04-21 | 福建师范大学 | Attached growth device for turtle-foot larva |
CN210538208U (en) * | 2019-08-01 | 2020-05-19 | 福建师范大学 | Step culture pond for inducing settlement and metamorphosis of turtle-foot adeps |
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2020
- 2020-07-15 CN CN202010678044.5A patent/CN111771806B/en active Active
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CN101578967A (en) * | 2009-06-16 | 2009-11-18 | 中国科学院南海海洋研究所 | Method for culturing cyprids of balanus reticulates on large scale |
EP3372073A1 (en) * | 2017-03-09 | 2018-09-12 | Universidade de Évora | Device and process for fixation of larvae and growth of juveniles of the stalked barnacle pollicipes pollicipes |
CN206713854U (en) * | 2017-05-06 | 2017-12-08 | 杨书戈 | A kind of leech children's leech attachment base made using waste or used plastics bucket |
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