CN113243341A - Barnacle larva breeding device, barnacle larva breeding method, antifouling test system, breeding-antifouling test system and antifouling test method - Google Patents
Barnacle larva breeding device, barnacle larva breeding method, antifouling test system, breeding-antifouling test system and antifouling test method Download PDFInfo
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Abstract
The invention discloses a barnacle larva breeding device which comprises a main cylinder body, an algae adding module and a water driving module, wherein the algae adding module is used for adding algae liquid to barnacle larvae, the water driving module is used for driving water in the main cylinder body to flow, a suspension isolation box used for accommodating the barnacle larvae is arranged in the water in the main cylinder body in a suspension mode, sieve holes are formed in the suspension isolation box, and an algae outlet of the algae adding module is communicated with the suspension isolation box. The invention also provides a barnacle larva breeding method, a barnacle cyprid larva antifouling test system capable of easily testing barnacle cyprid larva attachment behaviors, a barnacle cyprid larva breeding-antifouling test system and an antifouling test method. The barnacle larva breeding device and the barnacle larva breeding method have the advantages of high survival rate, high metamorphosis rate and the like. The culture device, the antifouling test system and the culture-antifouling test system have the advantages of simple structure, direct assembly, difficult damage, easy maintenance and the like.
Description
Technical Field
The invention belongs to the technical field of marine antifouling, and particularly relates to a culture device, a culture method, an antifouling test system, a culture-antifouling test system and an antifouling test method for culturing barnacle larvae.
Background
With the increasing frequency of human marine activities and the construction of large-scale marine engineering, the influence of marine fouling organisms is paid more attention, and the marine antifouling technology is more and more paid attention. Humans have attempted to explore how to reduce the effects of marine biofouling organisms using physical, chemical, and biological methods, among others. The physical method has the defects of complex process, high manufacturing cost and the like, and is not applied to large-scale practical application. The antifouling agents used in the chemical method have biological toxicity and have the problem of environmental pollution. Biological methods are extremely limited in their effectiveness. The rapid and effective evaluation of the antifouling materials and the technical efficiency is the key of research and development. In the test and evaluation of the marine antifouling technology, materials are usually screened through an indoor evaluation test, and then the actual sea hanging plate is used for final antifouling efficiency evaluation. At present, barnacles are the most main strong fouling organisms in indoor evaluation tests, and acinetocerous larvae are often used as model organisms for testing.
However, the indoor evaluation test of barnacle cyprid also has the following problems: 1. barnacle cyprid larvae were difficult to obtain for testing. In the prior art, in general, in dark conditions, a static water-sealed small container is adopted to culture barnacle nauplii, and high-density algae liquid is put into the barnacle nauplii (see 200910040284.6, 201210200636.1, 201711330730.8 and the like). The method has a series of technical difficulties of low survival rate, low metamorphosis rate, difficult collection and the like. 2. It is difficult to construct an environment favorable for barnacle cyprid larvae to attach and normally live. Therefore, the culture-antifouling test system which is beneficial to culture, survival and attachment of barnacle cyprid larvae, does not cause barnacle cyprid larva loss and is convenient for observing the attachment behavior of barnacle cyprid larvae has great significance.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects and defects mentioned in the background technology, and provide a barnacle larva breeding device and a barnacle larva breeding method which are easy to breed barnacle larvae, a barnacle cyprid larva antifouling test system and a barnacle cyprid larva breeding-antifouling test system which are easy to test barnacle cyprid larva attachment behaviors, and an antifouling test method. In order to solve the technical problems, the technical scheme provided by the invention is as follows:
the utility model provides a breeding device of barnacle larva, includes the master cylinder body, be used for throwing add algae liquid (high density algae liquid) for the barnacle larva add the algae module and be used for driving the water drive module that the water in the master cylinder body flows, the suspension is equipped with the suspension isolation box that is used for holding the barnacle larva in the water in the master cylinder body, be equipped with the sieve mesh on the suspension isolation box, add the play algae mouth of algae module with the box intercommunication is kept apart in the suspension. The algae adding module comprises a high-density algae liquid bottle, an intelligent titration pump and an algae conveying pipe, wherein an inlet of the algae conveying pipe is connected with the high-density algae liquid bottle, and an outlet of the algae conveying pipe is arranged in the suspension isolation box and can provide high-density algae liquid for barnacle larvae. The intelligent titration pump can set the starting time and the pumping volume through the mobile phone app.
In the above-mentioned breeding device, preferably, the suspension isolation box includes a buoyancy layer (such as a circle of foam) and a box body (which can adopt a round transparent acrylic tube) arranged below the buoyancy layer, the sieve pores are arranged at the bottom of the box body, and the pore size of the sieve pores is smaller than that of barnacle larvae. The side of the suspension isolation box can be made of transparent acrylic materials, and only the bottom of the suspension isolation box can realize microcirculation with a system water body by using a nylon screen (such as a nylon screen). Above-mentioned box is kept apart in suspension locates the water through buoyancy layer suspension, can guarantee that the barnacle larva is in the water always, when having the water level change that needs to change system water level or other reasons cause the system water level change, can guarantee the invariable loss of having avoided the barnacle larva of inside water level because of the suspension effect, can avoid the water to increase or reduce the influence to the barnacle larva. The mesh size of the mesh can be 300 meshes (48 μm), the body length of the newly born barnacle nauplii is about 200 μm, the body width is about 120 μm, and the barnacle nauplii cannot pass through the mesh. The predominant food rhodophyta larvae are about 10 μm long but do not escape rapidly from the sieve due to their long flagella and the absence of strong water flow in the suspended separator box.
In the above-mentioned breeding device, preferably, the main tank body is divided into a plurality of sub-tank bodies by a perforated partition component (such as an acrylic frame +200 mesh screen, the 200 mesh screen is located in the middle of the acrylic frame, which can limit the transfer of barnacle cyprid larvae and does not affect the flow of water); the upper part of the partition component with the holes is provided with a partition plate without holes, and the water level in the main cylinder body is ensured not to be higher than the top of the partition plate and not lower than the bottom of the partition plate. Divide into a plurality of branch cylinder bodies with the main cylinder body and can place a plurality of suspension isolation boxes simultaneously and breed and test, efficiency is higher. And, the existence of division board on the foraminiferous partition unit can also play the effect of preventing ripples, makes the suspension isolation box can maintain stable surface of water, is convenient for observe, and can prevent that the surface of water from surging to cause the suspension isolation box to rock and make the larva produce the loss, avoids surging in the follow-up test procedure to cause the surface of water uneven to lead to barnacle gland mesocarp larva to be beaten the jar wall and produce the loss.
In the above-mentioned breeding device, preferably, the water driving module includes a pump and a water pipe connected to a water outlet of the pump, the pump is disposed on one side of the main tank body, and the water outlet of the water pipe is located on the other side of the main tank body. The water filter can ensure that water flows from one side of the main cylinder body to the other side so as to drive all water in the main cylinder body to be in a forced flowing state, can be convenient for the subsequent water body filtering and purifying module to play a role in increasing oxygen. More preferably, two sub-cylinder bodies are arranged on two sides of the main cylinder body, the suspension isolation boxes are not arranged in the two sub-cylinder bodies, and water outlets of the pump and the water delivery pipe are respectively arranged to be matched with the isolation plate to avoid the influence of waves.
In the above-mentioned culture device, preferably, the culture device further includes a water body filtering and purifying module, a water body heating module and a water body supplementing module. The water body filtering and purifying module can comprise a wall-mounted waterfall filter and an externally-mounted UAS algae box, wherein the wall-mounted waterfall filter establishes a stable nitrification system which is enough to filter and purify harmful substances in small water bodies, convert the harmful substances into nutritive salts and maintain the stability of water quality. The outer hanging UAS algae box is used for quickly absorbing nutrient salts generated in the wall-mounted waterfall filter and is a built-in light source, and the cultivation of barnacle larvae can not be influenced. The wall-mounted waterfall filter and the externally-mounted UAS algae box are common equipment in the field. The water body heating module (such as a heating rod) provides a constant temperature environment for the system, and can perform test evaluation of the antifouling technology at different temperatures and constant temperatures. And because the optimum temperature for culturing the larvae is 30 ℃, a refrigerator is basically not needed, a low-cost heating rod is directly used, and the cost is lower. The water body supplement module (such as a water replenisher) supplements evaporated water for the system and keeps the salinity of the water body constant.
As a general technical concept, the present invention also provides a barnacle larva cultivation method using the above barnacle larva cultivation device, comprising the steps of:
s1: placing barnacle nauplii in the suspension isolation box, and then placing the suspension isolation box in the main cylinder body;
s2: starting the water driving module to drive water to flow circularly, and starting the algae adding module at regular time to add algae liquid (high-density algae liquid) into the suspension isolation box to provide baits for barnacle nauplii so that the barnacle nauplii are transformed into barnacle acinar mesolarvae;
s3: and after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box, namely finishing the barnacle larva culture process.
As a general technical concept, the invention also provides an antifouling test system for barnacle cyprid larvae, which comprises a cylinder body for accommodating the barnacle cyprid larvae cultured by the culture device and a water driving module for driving a water body in the cylinder body to flow, wherein an antifouling test device for adhesion of the barnacle cyprid larvae is arranged in the cylinder body. The main cylinder body can be adopted as the cylinder body, and the barnacle cyprid larvae of the suspension isolation box are directly poured into the main cylinder body in situ and then put into the antifouling testing device for testing. Above-mentioned cylinder body also can adopt other cylinder bodies, and it can to put into antifouling testing arrangement again and test this moment after transferring the barnacle gland mesolarva of box is kept apart in the suspension to other cylinder bodies.
As a general technical concept, the invention further provides a barnacle cyprid larva cultivation-antifouling test system, which comprises the cultivation device and an antifouling test device for barnacle cyprid larva attachment, wherein the antifouling test device is placed in the main cylinder body after the cultivation of the cultivation device is completed, the bathing module is closed, and the barnacle cyprids of the suspension isolation box are poured into the main cylinder body in situ.
In the above cultivation-antifouling test system, preferably, the antifouling test device includes a fixing rod, a clamp, and a test board for barnacle cyprid larvae attachment, the test board is connected to the fixing rod through the clamp, and the fixing rod is erected on the upper edge of the main cylinder body. Through the antifouling testing arrangement of above-mentioned structural style, can conveniently carry out the antifouling test of barnacle glandular scale larva, simple structure easily assembles, observes.
In the above cultivation-antifouling test system, preferably, the porous separation component, the inner wall of the main cylinder body and the inner wall of the suspension isolation box are all provided with an antifouling coating (such as PDMS and polydimethylsiloxane), so that barnacle cyprids can be effectively prevented from attaching to the cylinder wall and the porous separation component to affect the collection and test of barnacle larvae. The main cylinder body is made of transparent materials, so that the attachment behavior of barnacle cyprid larvae can be observed conveniently.
As a general technical concept, the present invention further provides an antifouling test method using the barnacle cyprid larvae breeding-antifouling test system, comprising a barnacle cyprid larvae breeding process and a barnacle cyprid larvae antifouling test process, wherein the barnacle larva breeding process comprises the following steps:
s1: placing barnacle nauplii in the suspension isolation box, and then placing the suspension isolation box in the main cylinder body;
s2: starting the water driving module to drive water to flow circularly, and starting the algae adding module regularly to add high-density algae liquid into the suspension isolation box to provide baits for barnacle nauplii so that the barnacle nauplii are transformed into barnacle acinus larvae;
s3: after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box, namely finishing the barnacle larva culture process;
the barnacle cyprid larva antifouling test process comprises the following steps:
s4: closing the algae adding module, and setting technological parameters in the main cylinder body in the antifouling test process;
s5: and pouring barnacle cyprid larvae in the suspension isolation box into the main cylinder body in situ, putting the main cylinder body into the antifouling testing device, and observing the attachment behavior of the barnacle cyprid larvae on the antifouling testing device to finish the antifouling testing process of the barnacle cyprid larvae.
In the barnacle cyprid larva antifouling test process, if necessary, adult barnacles can be placed in water outside a test area to release the water-soluble pheromone WPS, and the barnacle cyprid larva antifouling test method is favorable for inducing attachment of barnacle cyprid larvae. The dynamic process between barnacle cyprid and test board is observed by high speed camera or naked eye, and the effect of anti-fouling technique is evaluated by the attachment rate and attachment period.
In the invention, in the culturing and testing process of barnacle larvae, the salinity of artificial seawater is 30-33 per mill, the pH value is 7.8-8.2, the KH value is 8-10, the temperature is 30 ℃, the illumination from 8 o 'clock in the day to 20 o' clock in the night is 500lx (the illumination intensity can not cause the aggregation of larvae), and the night is in a dark state. In the barnacle acinus larva antifouling test process, the total water volume of a system is 10L, the volume of a test area is 2L, the flow of a main pump is 200L/h, the size of a test board is 120mm multiplied by 30mm multiplied by 2mm (height multiplied by width multiplied by thickness), the material is cement, glass, stainless steel, silica gel, PTFE, PVC and the like, 300 acinus larvae are transferred to the test area, and the test result represents the attachment rate and the attachment period.
In the prior art, in general, in a dark condition, a static water-sealed small container is adopted to culture barnacle nauplii, high-density algae liquid is thrown into the barnacle nauplii, metabolic products and culture medium residues of algae can bring huge damage to the barnacle nauplii due to the fact that the static water-sealed container has no metabolism and dilution functions, and microorganisms such as high-density algae and bacteria can grab a large amount of oxygen under the dark condition to influence the growth of the barnacle nauplii. The low-density algae liquid can prolong the growth and development period of barnacle nauplii due to low algae density, and improve the death rate. Barnacle larvae like to move around the edge of the wall of the aquarium on the water surface, and if the barnacle larvae are directly oxygenated, the barnacle nauplii are blown to the aquarium wall by bubbles to cause loss. In addition, the culture container in the prior art has no function of purifying water quality, the water changing is very difficult, the water changing can cause the continuous loss of the barnacle nauplii, and the continuous use of new water can generate very large stimulation to the barnacle nauplii. In addition, the collection of nauplii after culturing nauplii into acinetocerous larvae is also a difficult problem in the prior art, and a certain amount of loss is inevitable in the collection process.
The barnacle larva breeding device and the breeding method at least have the following advantages: 1. under the flowing water phase, a large amount of algal metabolites such as algal toxins and the like and culture medium residues brought by feeding of high-density algal liquid can be quickly diluted into a system from a suspension isolation box through Brownian motion to be purified. Metabolites of barnacle larvae and harmful products of decomposition of dead barnacle larvae are also diluted from the suspension cage into the system by brownian motion to be purified. The influence of high-density algae liquid adding or other wastes on barnacle larvae is avoided. And after the high-density algae liquid is added, due to the limiting effect of the suspension isolation box, the algae liquid can not be diffused and diluted quickly, the high-density algae liquid does not need to be added in a large amount like the prior art, and the stimulation of the high-density algae liquid to barnacle larvae can be reduced to the minimum by adopting a small-amount and repeated adding mode. 2. The water in the main cylinder body is originally an oxygen-enriched state, fine bubbles in the water can also float through the sieve mesh, the condition of still water oxygen deficiency cannot exist in the suspension isolation box, and the problem that the larvae are beaten out to lose by the bubbles due to direct inflation is avoided. 3. On one hand, the barnacle acinus larvae have much stronger movement capability than the nauplii larvae and have reduced phototaxis compared with the nauplii larvae, so that the barnacle acinus larvae are extremely difficult to collect. On the other hand, barnacle nauplii can undergo molting in the metamorphosis development process, and become glandular cyprids which are weaker than nauplii after molting, so that the loss and activity of the glandular cyprids are reduced due to collection. According to the invention, after the barnacle larvae are cultured and developed to be metamorphosis larvae, the suspension isolation box is directly taken out for transfer, and the collection step is omitted. 4. The culture device has the function of purifying water quality, can provide stable and good water quality, does not need to change water, avoids continuous loss of barnacle nauplii caused by changing water every day, avoids extremely large stimulation to the barnacle nauplii caused by continuously using new water, and does not need any manual operation in the whole process from culturing the barnacle nauplii to the cyprid larvae. Generally speaking, the culture device and the culture method have a series of advantages of high survival rate, high metamorphosis rate, less loss, easiness in collection and the like, and lay a foundation for simply, conveniently and efficiently culturing barnacle nauplii larvae into the cyprid larvae indoors and for deeply understanding the behavioristics and adhesion mechanism research of the barnacle cyprid larvae.
The antifouling test system for barnacle cyprid larvae creates a good indoor living environment for barnacle cyprid larvae, is beneficial to adhesion of barnacle cyprid larvae, is convenient to control the synergistic effect of single variable and multivariable (such as temperature, water flow and the like), avoids loss of larvae, can test and evaluate the effect and the application range of an antifouling technology by changing physical and chemical parameters and using the adhesion rate and the adhesion period of the barnacle cyprid larvae, and greatly reduces the time cost and the economic cost of antifouling test and evaluation. In addition, the system can also clearly observe the dynamic process of barnacle cyprid adhesion, is convenient for observing the dynamic process of barnacle cyprid under the influence of various anti-fouling technologies, and further deeply develops the behavioral research of barnacle cyprid adhesion.
The barnacle cyprid breeding-antifouling test system and the antifouling test method have the advantages of the barnacle cyprid breeding device and the antifouling test system, can realize automatic barnacle cyprid-to-cyprid breeding, can perform barnacle cyprid-to-cyprid adhesion experiment in situ, have high final survival adhesion rate, and can be used for barnacle cyprid-to-cyprid cultivation, barnacle cyprid adhesion test under different physical and chemical conditions, and adhesion collection of barnacle cyprids and other aquatic organisms. The requirement of researching barnacle acinetobacter larvae indoors is met, a specific thought method is provided for the design of other aquatic organism seedling culture systems, and the method plays a guiding role in building various indoor scientific research platforms needing to simulate marine environments.
Compared with the prior art, the invention has the advantages that:
1. according to the device and the method for culturing the barnacle larvae, the suspension isolation box for containing the barnacle larvae is in a flowing water state, and the added algae liquid, algal metabolites such as algal toxins harmful to the barnacle larvae and culture medium residues can be quickly diluted into a system from the sieve pores of the suspension isolation box to be purified, so that the influence of the addition of the high-density algae liquid on the barnacle larvae is avoided. In addition, the water in the main tank body is in a flowing state, so that the oxygen content in the water body can be improved, and the metamorphosis development of barnacle larvae is facilitated. The breeding device and the breeding method have the advantages of high survival rate, high metamorphosis rate and the like.
2. According to the culture device and the culture method, the barnacle larvae are placed in the suspension isolation box for culture, the loss of the barnacle larvae caused by manual operation in the whole culture process is avoided, collection is not needed after the culture is finished, the barnacle larvae can be directly moved for subsequent experiments, the transfer step is omitted, and the work of collecting the barnacle larvae is greatly reduced.
3. The antifouling test system for barnacle cyprid larvae creates a good indoor living environment for barnacle cyprid larvae, is beneficial to adhesion of barnacle cyprid larvae, can clearly observe the dynamic process of adhesion of barnacle cyprid larvae, greatly reduces the time cost and the economic cost of antifouling test evaluation, and is convenient for developing the behavioral research on barnacle cyprid larvae adhesion.
4. The barnacle cyprid breeding-antifouling test system and the antifouling test method have the functions of breeding and testing, can be used for cultivating barnacle nauplii to cyprids and testing barnacle cyprid adhesion under different physical and chemical conditions, and play a guiding role in building various indoor scientific research platforms needing to simulate marine environments.
5. The culture device, the antifouling test system and the culture-antifouling test system have the advantages of simple structure, direct assembly, difficult damage, easy maintenance and the like, and the culture method and the antifouling test method have the advantages of full automation, less manual operation and the like.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural view of a barnacle larva culturing device (small oval in the figure is barnacle nauplii).
Fig. 2 is a schematic structural diagram of the barnacle cyprid anti-fouling test system of the invention (small oval in the figure is barnacle cyprid).
FIG. 3 is a schematic structural diagram of the antifouling test apparatus according to the present invention.
Illustration of the drawings:
1. a main cylinder body; 2. adding an algae module; 201. high density algae solution bottle; 202. an intelligent titration pump; 203. an algae conveying pipe; 3. a water driving module; 301. a pump; 302. a water delivery pipe; 4. a suspension isolation box; 401. a buoyancy layer; 402. a box body; 5. a perforated partition assembly; 501. a separator plate; 601. a wall-mounted waterfall filter; 602. UAS algae box; 7. a water body heating module; 8. a water body supplement module; 9. an antifouling test device; 901. fixing the rod; 902. a clamp; 903. and (6) testing the board.
Detailed Description
In order to facilitate an understanding of the invention, the invention will be described more fully and in detail below with reference to the accompanying drawings and preferred embodiments, but the scope of the invention is not limited to the specific embodiments below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
Example (b):
as shown in fig. 1, the breeding device of barnacle larva of this embodiment, including main cylinder body 1, be used for throwing the algae module 2 that adds of high density algae liquid and be used for driving the water drive module 3 that the water in the main cylinder body 1 flows for the barnacle larva, the suspension is equipped with the suspension isolation box 4 that is used for holding the barnacle larva in the water in the main cylinder body 1, is equipped with the sieve mesh on the suspension isolation box 4, adds the play algae mouth and the suspension isolation box 4 intercommunication of algae module 2. The first algae adding module 2 comprises a high-density algae liquid bottle 201, a first intelligent titration pump 202 and a first algae conveying pipe 203, wherein an inlet of the first algae conveying pipe 203 is connected with the high-density algae liquid bottle 201, and an outlet of the first algae conveying pipe 203 is arranged in the suspension isolation box 4 and can provide high-density algae liquid for barnacle larvae. The first smart titration pump 202 may set the start time and draw volume via the mobile phone app.
In this embodiment, the suspension isolation box 4 comprises a buoyancy layer 401 and a box 402 arranged below the buoyancy layer 401, the sieve holes are arranged at the bottom of the box 402, and the size of the sieve holes (if 300 meshes are adopted) is smaller than that of barnacle larvae.
In the embodiment, the main cylinder body 1 is divided into a plurality of sub cylinder bodies by the separation component 5 with holes; the upper part of the perforated partition member 5 is a partition plate 501 which is not perforated, and it is ensured that the water level in the main cylinder 1 is not higher than the top of the partition plate 501 nor lower than the bottom of the partition plate 501. Specifically, as shown in fig. 1, the main cylinder body 1 is divided into 5 sub-cylinder bodies by 4 perforated partition assemblies 5, the middle 3 sub-cylinder bodies are used for placing the suspension isolation boxes 4 to culture barnacle larvae, and the two sub-cylinder bodies at the two ends are used for placing other accessories, such as a water driving module 3, a water body filtering and purifying module, a water body heating module 7, a water body supplementing module 8 and the like.
In this embodiment, the water driving module 3 includes a pump 301 and a water pipe 302 connected to a water outlet of the pump 301, the pump 301 is disposed on one side of the main cylinder 1, and the water outlet of the water pipe 302 is located on the other side of the main cylinder 1.
In this embodiment, the cultivation device further includes a water filtering and purifying module, a water heating module 7 and a water supplementing module 8. Specifically, in this embodiment, the water filtering and purifying module includes a wall-mounted waterfall filter 601 and an external-mounted UAS algae box 602 (both of the above-mentioned devices are conventional devices), the water heating module 7 may employ a heating rod with a low price, and the water replenishing module 8 may employ a water replenishing device.
The culture method for culturing the barnacle larvae by using the culture device for the barnacle larvae comprises the following steps:
s1: the barnacle nauplii are placed in the suspension isolation box 4, and then the suspension isolation box 4 is placed in the main cylinder body 1;
s2: starting a water driving module 3 to drive water to circularly flow, and starting an algae adding module 2 at regular time to add high-density algae liquid into a suspension isolation box 4 to provide baits for barnacle nauplii so that the barnacle nauplii are transformed into barnacle acinus larvae;
s3: and after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box 4, namely finishing the barnacle larva culture process.
In this embodiment, a specific cultivation process is taken as an example to illustrate the cultivation process parameters as follows: 10L of water in the main tank body 1, 9 suspension isolation boxes 4, 40mL of volume of the suspension isolation boxes 4, 1.5 cultivation density/mL, the bait is Platymonas subcordiformis with density of 3.0 multiplied by 106cell/mL, the feeding period is controlled to be 2 hours, the feeding amount is 1mL, the temperature is 30 ℃, the salinity is 30 per thousand, and the illumination from 8 days to 20 nights500lx, dark state for the rest of the time.
As shown in fig. 2, the antifouling test system of barnacle acinus larvae of this embodiment includes the cylinder body that is used for the barnacle acinus larvae that above-mentioned breeding device of holding bred to obtain and the water drive module 3 that is used for driving the water in the cylinder body to flow, is equipped with in the cylinder body to be used for the adnexed antifouling test device 9 of barnacle acinus larvae. The cylinder body can adopt the main cylinder body 1 or other cylinder bodies, and if the cylinder body can adopt the main cylinder body 1, the structure is the same as that of the culture-antifouling test system in the following.
As shown in fig. 2, the barnacle cyprid larva cultivation-antifouling test system of the embodiment includes the cultivation device and the antifouling test device 9 for barnacle cyprid larva attachment, and the antifouling test device 9 is placed in the main cylinder body 1 after the cultivation of the cultivation device is completed, the algae adding module is removed, and the barnacle cyprids of the suspension isolation box 4 are poured into the main cylinder body 1 in situ.
As shown in fig. 3, in this embodiment, the antifouling test device 9 includes a fixing rod 901, a clamp 902 and a test board 903 for barnacle cyprid larvae attachment, the test board 903 is connected to the fixing rod 901 through the clamp 902, and the fixing rod 901 is erected on the upper edge of the main cylinder 1. The test board 903 may be a plurality of test boards, and each of the sub-cylinder bodies may be provided with one corresponding anti-fouling test device 9.
The antifouling test method using the barnacle cyprid breeding-antifouling test system comprises a barnacle cyprid breeding process and a barnacle cyprid antifouling test process, wherein the barnacle cyprid breeding process comprises the following steps:
s1: the barnacle nauplii are placed in the suspension isolation box 4, and then the suspension isolation box 4 is placed in the main cylinder body 1;
s2: starting a water driving module 3 to drive water to circularly flow, and starting an algae adding module 2 at regular time to add high-density algae liquid into a suspension isolation box 4 to provide baits for barnacle nauplii so that the barnacle nauplii are transformed into barnacle acinus larvae;
s3: after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box 4, namely finishing the barnacle larva culture process;
the barnacle acinar larva antifouling test process comprises the following steps:
s4: closing the algae adding module 2, and setting technological parameters (including temperature, salinity, water flow, illumination intensity and the like) in the main cylinder body 1 in the antifouling test process;
s5: pouring barnacle cyprid larvae in the suspension isolation box 4 into the main cylinder body 1 in situ, putting the antifouling testing device 9 (the material of the testing plate 903 is selected, the included angle between the testing plate 903 and the water flow direction is adjusted in the putting process), and observing the attachment behavior of barnacle cyprid larvae on the antifouling testing device 9, namely completing the antifouling testing process of barnacle cyprid larvae.
In this example, if a 5mL pasteur burette is used to transfer barnacle cyprid larvae to the test area, the attrition rate is 15%, while the attrition rate is less than 2% when the suspension isolation box 4 is directly poured into the test area.
The present embodiment illustrates the following process parameters by taking a specific test procedure as an example: the volume of a single test area is 2L, 100 barnacle cyprid larvae are thrown in, the test board 903 is a cement sheet, the temperature is controlled to be 30 ℃, the salinity is 30 per thousand, the illumination from 8 days to 20 nights is 500lx, the rest time is in a dark state, the water flow is 200L/h, and the area of the screen (a perforated area in the middle of the isolation board 501) is 0.02m2。
Claims (10)
1. The utility model provides a breeding device of barnacle larva, its characterized in that, including main cylinder body (1), be used for throwing add algae liquid add algae module (2) and be used for driving for the barnacle larva water drive module (3) that the water in main cylinder body (1) flows, suspension is equipped with suspension isolation box (4) that are used for holding barnacle larva in the water in main cylinder body (1), be equipped with the sieve mesh on suspension isolation box (4), add the play algae mouth of algae module (2) with suspension isolation box (4) intercommunication.
2. The cultivating device according to claim 1, wherein the suspension isolation box (4) comprises a buoyancy layer (401) and a box body (402) arranged below the buoyancy layer (401), the sieve holes are arranged at the bottom of the box body (402), and the size of the sieve holes is smaller than that of barnacle larvae.
3. A cultivating device according to claim 1, characterised in that the main cylinder (1) is divided into several sub-cylinders by perforated partition elements (5); the upper part of the perforated partition component (5) is provided with a partition plate (501) without an opening, and the water level in the main cylinder body (1) is ensured not to be higher than the top of the partition plate (501) and not lower than the bottom of the partition plate (501).
4. A cultivating device according to any one of claims 1-3, characterised in that the water-driving module (3) comprises a pump (301) and a water duct (302) connected to the water outlet of the pump (301), the pump (301) being arranged on one side of the main tank (1), the water outlet of the water duct (302) being arranged on the other side of the main tank (1).
5. An aquaculture device according to any one of claims 1-3, further comprising a water body filtration and purification module, a water body heating module (7) and a water body replenishment module (8).
6. A method of rearing barnacle larvae using the rearing device of barnacle larvae according to any one of claims 1 to 5, comprising the steps of:
s1: placing barnacle nauplii in the suspension isolation box (4), and then placing the suspension isolation box (4) in the main cylinder body (1);
s2: the water driving module (3) is started to drive water to flow circularly, the algae adding module (2) is started regularly to add algae liquid into the suspension isolation box (4) to provide baits for barnacle nauplii, and the barnacle nauplii are transformed into barnacle acinar cyprids;
s3: and after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box (4), namely finishing the barnacle larva culture process.
7. An antifouling test system for balanus calmette-guerin larvae, which is characterized by comprising a cylinder body for containing the balanus calmette-guerin larvae cultured by the culture device according to any one of claims 1-5 and a water driving module (3) for driving a water body in the cylinder body to flow, wherein an antifouling test device (9) for the balanus calmette-guerin larvae to adhere to is arranged in the cylinder body.
8. A barnacle cyprid larvae farming-antifouling test system comprising the farming device of any one of claims 1 to 5 and an antifouling test device (9) for barnacle cyprid larvae attachment, wherein the antifouling test device (9) is placed in the main tank body (1) after the farming of the farming device is completed and the barnacle cyprids of the suspension cage (4) are poured in situ into the main tank body (1).
9. A farming-antifouling test system according to claim 8, wherein the antifouling test device (9) comprises a fixed rod (901), a clamp (902) and a test plate (903) for barnacle cyprid larvae attachment, the test plate (903) is connected with the fixed rod (901) through the clamp (902), and the fixed rod (901) is erected on the upper edge of the main cylinder (1).
10. A method for antifouling testing using the barnacle cyprid larvae rearing-antifouling test system according to claim 8 or 9, comprising a barnacle larva rearing process and a barnacle cyprid larva antifouling test process, said barnacle larva rearing process comprising the steps of:
s1: placing barnacle nauplii in the suspension isolation box (4), and then placing the suspension isolation box (4) in the main cylinder body (1);
s2: the water driving module (3) is started to drive water to flow circularly, the algae adding module (2) is started regularly to add algae liquid into the suspension isolation box (4) to provide baits for barnacle nauplii, and the barnacle nauplii are transformed into barnacle acinar cyprids;
s3: after the culture is finished, collecting barnacle cyprid larvae from the suspension isolation box (4), namely finishing the barnacle larva culture process;
the barnacle cyprid larva antifouling test process comprises the following steps:
s4: closing the algae adding module (2), and setting technological parameters in the main cylinder body (1) in the antifouling test process;
s5: and pouring barnacle cyprid larvae in the suspension isolation box (4) into the main cylinder body (1) in situ, putting the main cylinder body into the antifouling testing device (9), and observing the attachment behavior of the barnacle cyprid larvae on the antifouling testing device (9), namely completing the barnacle cyprid larva antifouling testing process.
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| CN102687708A (en) * | 2012-06-18 | 2012-09-26 | 中国船舶重工集团公司第七二五研究所 | Integrated control method for culture of barnacle larva |
| CN105724293A (en) * | 2016-03-08 | 2016-07-06 | 临沂大学 | Method for high-density culture of perna viridis larvae through medical stone bucket |
| CN110419475A (en) * | 2019-08-01 | 2019-11-08 | 福建师范大学 | A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687708A (en) * | 2012-06-18 | 2012-09-26 | 中国船舶重工集团公司第七二五研究所 | Integrated control method for culture of barnacle larva |
| CN105724293A (en) * | 2016-03-08 | 2016-07-06 | 临沂大学 | Method for high-density culture of perna viridis larvae through medical stone bucket |
| CN110419475A (en) * | 2019-08-01 | 2019-11-08 | 福建师范大学 | A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method |
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