CN110250514A - Health food of functions of removing chloasma and preparation method thereof - Google Patents
Health food of functions of removing chloasma and preparation method thereof Download PDFInfo
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- CN110250514A CN110250514A CN201910584681.3A CN201910584681A CN110250514A CN 110250514 A CN110250514 A CN 110250514A CN 201910584681 A CN201910584681 A CN 201910584681A CN 110250514 A CN110250514 A CN 110250514A
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- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 210000000216 zygoma Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses health foods of functions of removing chloasma and preparation method thereof, the health food of functions of removing chloasma calculates by weight, and active constituent includes: 200-280 parts of horse lyophilized powder of deer fetus, 1000-1400 parts of Radix Salviae Miltiorrhizae, 1000-1400 parts of pueraria lobata, 600-1000 parts of Radix Paeoniae Alba and 20-30 parts of isoflavones.Health food prepared by the present invention has the characteristics that have no toxic side effect, freckle effect is significant, uneasy to recur.
Description
Technical field
The present invention relates to technical field of health care food.It is more particularly related to the health food of functions of removing chloasma and
Preparation method.
Background technique
For " Chinese skin typology " studies have shown that for Chinese women, first " killer " of skin is not wrinkle,
But color spot, and most multiple in color spot is exactly chloasma;Chloasma can be with the passage at age, according to climatic environment and sunlight
The difference of exposure degree and change different;Women will appear chloasma due to family, operating pressure etc. after 30 on the face,
Women beauty is seriously affected, causes physical and psychological pressure to women.Chloasma treatment is always to perplex the problem of skin circle.Many factors
Can cause chloasma, if long term ultraviolet irradiation, oral contraceptive, gestation, endocrine, some drugs, poor quality cosmetics,
Heredity, race, nutrition, liver diseases, dyssecretosis etc. pursue natural beauty agitation and more and more people are added
Process to the ranks for finding nti-freckle product, chloasma formation is complex, not only will be from inhibiting in appearance to it
And treatment, more should Studying carefully its reason, to causing the factor of chloasma to be inhibited and adjusted, take away the firewood under the cauldron from vivo, from basic
The upper puzzlement far from chloasma.Product there is the effects poor, slow effect of existing treatment functions of removing chloasma have side effect, easily
The problems such as recurrence.
Summary of the invention
The object of the present invention is to provide health food of functions of removing chloasma and preparation method thereof, have have no toxic side effect, curative effect
Good, quick, uneasy to recur feature.
In order to realize these purposes and other advantages according to the present invention, the health food of functions of removing chloasma is provided, by weight
It measures part to calculate, active constituent includes:
200-280 parts of horse lyophilized powder of deer fetus, 1000-1400 parts of Radix Salviae Miltiorrhizae, 1000-1400 parts of pueraria lobata, 600-1000 parts of Radix Paeoniae Alba
With 20-30 parts of isoflavones.
It preferably, further include pharmaceutically acceptable auxiliary material in the health food of the functions of removing chloasma.
Preferably, it in the health food of the functions of removing chloasma, calculates by weight, auxiliary material includes:
600-1000 parts of dextrin, 110-130 parts of mannitol and 7-13 parts of sodium carboxymethylcellulose.
Preferably, it in the health food of the functions of removing chloasma, calculates by weight, active constituent further include: the leaf of bamboo
25-45 parts of extract, 10-20 parts of Flos Rosae Rugosae extract and 20-30 parts of Seabuckthorm Seed Oil;
It calculates by weight, auxiliary material further include: 30-100 parts of starch.
The preparation method of the health food of functions of removing chloasma, comprising the following steps:
Step 1: by the Radix Salviae Miltiorrhizae of 1000-1400 parts by weight, the pueraria lobata of 1000-1400 parts by weight, 600-1000 parts by weight
Radix Paeoniae Alba is extracted 1-3 times with the alcohol reflux that volume fraction is 50-70%, and 1-3 hours each, each solid-liquid ratio is 1:8-15g/
ML is 55-65 DEG C in temperature after merging filtrate, and negative pressure is to be concentrated under reduced pressure under -0.06~-0.08Mpa, after recycling ethyl alcohol, then
Temperature is 65-75 DEG C, when negative pressure is that be concentrated under reduced pressure into relative density under -0.06~-0.08Mpa be 1.25-1.30 (60 DEG C), is obtained
Medicinal extract;
Step 2: by medicinal extract be 65-75 DEG C in temperature at it is dry after, put to room temperature, later crush dry cream, cross 80 mesh
Sieve, obtains extract powder;
Step 3: remaining supplementary material is used 60-120 mesh sieve respectively, weigh later;Take the soybean of 20-30 parts by weight
Isoflavones, the sodium carboxymethylcellulose of 7-13 parts by weight are uniformly mixed, cross 60-80 mesh, obtain mixed powder I;Mixed powder I again with etc.
The mannitol of amount is uniformly mixed, and is crossed 60-80 mesh, is obtained mixed powder II;Mixed powder II is uniformly mixed with remaining mannitol again,
60-80 mesh is crossed, mixed powder III is obtained;
Step 4: by mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 200-280 parts by weight and 600-1000 parts by weight
Dextrin investment mixing machine in mix 20-40min, obtain total mixed powder, quality is added while stirring into mixing machine later is
The ethyl alcohol that the volume fraction of total mixing silty amount 10-40% is 80%, stirs 10-20min after adding, softwood is made;
Step 5: softwood is transferred to oscillating granulator, pelletized with 10-20 mesh, it is dry that wet granular is transferred to boiling later
In dry bed, drying at 55-65 DEG C to moisture content is 2.0-4.0%, and it is whole that the particle after drying is then crossed 10-20 mesh
Grain.
Preferably, in the preparation method of the health food of the functions of removing chloasma, in the step 4, by mixed powder
III, it is mixed in the dextrin investment mixing machine of extract powder, the horse lyophilized powder of deer fetus of 200-280 parts by weight and 600-1000 parts by weight
Afterwards, then into mixing machine the bamboo extractive of 25-45 parts by weight, the Flos Rosae Rugosae extract and 30-100 of 10-20 parts by weight are put into
The starch of parts by weight, mixes 20-40min in mixing machine, obtains total mixed powder, later into mixing machine while stirring with atomization
Form sprinkling quality be total mixing silty amount 10-40% the volume fraction ethyl alcohol for being 80% and 20-30 parts by weight sea-buckthorn
The mixed liquor of seed oil composition stirs 10-20min after having sprayed, softwood is made.
Preferably, in the preparation method of the health food of the functions of removing chloasma, the preparation side of the bamboo extractive
Method the following steps are included:
Step A, it by after fresh leaf of bamboo impurity elimination, sterilization, chopping, thaws at room temperature after the leaf of bamboo is freezed at -15 DEG C, weight
After multiple 2-3 time, mixed with water that yeast and weight that weight be leaf of bamboo weight 0.01-0.1% are 5-10 times of leaf of bamboo weight
It is even, it is sealed by fermentation 20-30h at 25-30 DEG C, obtains tunning one;
Step B, the cellulase that weight is one weight 0.5-1% of tunning, Yi Jichong are added into tunning one
Amount is the pectase of one weight 0.5-1% of tunning, digests 1-2h in the case where temperature is 40-50 DEG C, pH value is 5-6, later mistake
Filter, obtains filtrate one and filter residue one;
Step C, spraying by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
It is dry, obtain powder one;After filter residue one is dry, crushing, 100-150 mesh is crossed, the ethyl alcohol for being later 80% with volume fraction
Refluxing extraction 1-2 times, each 2-3h, each solid-liquid ratio are 1:10g/mL, after standing 10-12h, are with volume fraction again
80% alcohol reflux 1-2 times, each solid-liquid ratio are 1:8g/mL, are 55-65 DEG C in temperature after merging filtrate, and negative pressure is-
It is concentrated under reduced pressure under 0.06~-0.08Mpa, after recycling ethyl alcohol, spray drying obtains powder two;
Step D, powder one is mixed with powder two, obtains bamboo extractive.
Preferably, in the preparation method of the health food of the functions of removing chloasma, the preparation of the Flos Rosae Rugosae extract
Method the following steps are included:
Step 1), by after fresh roseleaf impurity elimination, sterilization, grinding, be rose weight 0.01-0.1%'s with weight
Yeast and weight are that the water of 5-10 times of rose weight is uniformly mixed, and ferment 1-2 days at 25-30 DEG C, obtain tunning
Two;
Step 2), into tunning two be added weight be two weight 0.5-1% of tunning cellulase, and
Weight is the pectase of two weight 0.5-1% of tunning, digests 2-3h in the case where temperature is 40-50 DEG C, pH value is 5-6, later
Filtering, obtains filtrate two and filter residue two;
Step 3), by filter residue two immerse weight be 10-15 times of two weight of filter residue 20-50 times of liquid of bamboo vinegar or wood vinegar
In, after sealing and standing 10-12h, flows back 1 time, after filtering, obtain filtrate three and filter residue three;
Step 4), by filter residue three immerse weight be 7-10 times of three weight of filter residue 20-50 times of liquid of bamboo vinegar or wood vinegar
In, after sealing and standing 1-2h, assisting ultrasonic extracts 20-30min at being 30-40 DEG C in temperature, filters later, obtains filtrate four;
Step 5), by filtrate two, filtrate three and filtrate four mix after, temperature be 65-75 DEG C, negative pressure be -0.06~-
After being concentrated under reduced pressure under 0.08Mpa, spray drying obtains Flos Rosae Rugosae extract.
Preferably, in the preparation method of the health food of the functions of removing chloasma, the step 2 specifically:
Medicinal extract is dispensed into each stainless steel disc, is put into 65-75 DEG C of baking oven, forced air drying 56-60h to feel dry cream
Harden, touching it is not viscous after take out, put to room temperature, left-hand thread stainless steel disc, tapping is tried to get to the heart of a matter to dry cream fall off after remove, collect dry cream,
Medicinal extract after drying is crushed, 80 meshes is crossed, obtains extract powder.
The present invention is include at least the following beneficial effects:
The present invention is definite with effect, the specific deer fetus powder of the mechanism of action, Radix Salviae Miltiorrhizae, pueraria lobata, Radix Paeoniae Alba, based on isoflavones
Raw material is wanted, is to develop foundation, genesis mechanism and rules for the treatment of prescription according to Chinese medicine to chloasma with modern pharmacology research result, fills
Divide and excavate its healthcare function, Multiple components, comprehensive function reach the healthcare function of eliminate chloasma.This product qi-restoratives promotes the production of body fluid, living
Blood promoting menstruation, nourishing blood for regulating menstruation keep qi and blood, train of thought unobstructed, are aided with adjusting endocrine, remove free radical, anti peroxidation of lipid, improve micro-
Circulatory function, to fundamentally be able to eliminate chloasma.For it is numerous have patient with chloasma provide one safely, effectively, can
Lean on, inexpensive health food is compared with other similar health-care foods, have certain comprehensive advantage.
Material characteristic in the present invention: red deer tire uses freeze-dried powder, remains original nutrition, and property to greatest extent
Matter is stablized, and is conducive to storage, and can be advantageously used in preparation compatibility;Isoflavones derives from soybean, rational technology, quality control standard
Strictly, quality is guaranteed.Characteristics of prescriptions: complete square compatibility is closed, and qi-restoratives promotes the production of body fluid, and blood circulation, nourishing blood for regulating menstruation keeps qi and blood, train of thought unobstructed,
It is aided with adjusting endocrine, removes free radical, anti peroxidation of lipid, improves microcirculation function.To reach the guarantor of eliminate chloasma
Strong effect.Formulation characteristic: the advantages that it is complete rapidly that there is granule liquid preparation to absorb, and is easy to take, in good taste, and have solid
The features such as body preparation quality is stablized, and carries, saves and is convenient to take.
Human feeding trial shows: after test-meal group takes particle 30 days of the preparation of embodiment 2 as required, area of chloasma is flat
Reduce 5.58 ± 3.42cm2, area is averagely reduced to 15.30% ± 9.17%;Lighter, colour atla averagely declines 0.38 ±
0.37 degree;In 50, effective 8, total effective rate 16.00%.Before and after test-meal, blood biochemistry, blood, urine, feces routine of trencherman etc. is tried
Every Index for examination illustrates that this product has no adverse effects to examination trencherman's health in normal range (NR);It is not seen during test-meal
Observe allergy and other adverse reactions.According to functions of removing chloasma function in " health food is examined and assessment technique specification " (version in 2003)
It can determine that standard, it is believed that particle prepared by the present invention has the function of functions of removing chloasma.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1
The health food of functions of removing chloasma, calculates by weight, and active constituent includes:
200 parts of horse lyophilized powder of deer fetus, 1000 parts of Radix Salviae Miltiorrhizae, 1000 parts of pueraria lobata, 600 parts of Radix Paeoniae Alba and 20 parts of isoflavones.
It further include pharmaceutically acceptable auxiliary material in the health food of the functions of removing chloasma.
It in the health food of the functions of removing chloasma, calculates by weight, auxiliary material includes:
600 parts of dextrin, 110 parts of mannitol and 7 parts of sodium carboxymethylcellulose.
A kind of preparation method of the health food of functions of removing chloasma, comprising the following steps:
Step 1: by the Radix Salviae Miltiorrhizae of 1000 parts by weight, the pueraria lobata of 1000 parts by weight, 600 parts by weight Radix Paeoniae Alba volume fraction
For 50% alcohol reflux extract 1 hour, solid-liquid ratio 1:8g/mL, later by filtrate temperature be 55-65 DEG C, negative pressure be-
It is concentrated under reduced pressure under 0.06~-0.08Mpa, is 65-75 DEG C in temperature, negative pressure is to subtract under -0.06~-0.08Mpa after recycling ethyl alcohol
Pressure be concentrated into relative density be 1.25-1.30 (60 DEG C) when, obtain medicinal extract;
Step 2: dispensing medicinal extract into each stainless steel disc, it is put into 65 DEG C of baking oven, forced air drying 56h is dry to feel
Cream hardens, touching it is not viscous after take out, put to room temperature, left-hand thread stainless steel disc, tapping is tried to get to the heart of a matter to dry cream fall off after remove, collect dry
Cream crushes the medicinal extract after drying, crosses 80 meshes, obtains extract powder;
Step 3: remaining supplementary material is used 60 mesh sieves respectively, weigh later;Take 20 parts by weight isoflavones, 7
The sodium carboxymethylcellulose of parts by weight is uniformly mixed, and is crossed 60 meshes, is obtained mixed powder I;Mixed powder I is mixed with the mannitol of equivalent again
Uniformly, 60 meshes are crossed, mixed powder II is obtained;Mixed powder II is uniformly mixed with remaining mannitol again, 60 meshes is crossed, obtains mixed powder
Ⅲ;
Step 4: the dextrin of mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 200 parts by weight and 600 parts by weight is put into
20min is mixed in mixing machine, obtains total mixed powder, and it is total mixing silty amount that quality is added while stirring into mixing machine later
The ethyl alcohol that 10% volume fraction is 80%, stirs 10min after adding, and softwood is made;
Step 5: softwood is transferred to oscillating granulator, is pelletized with 10 meshes, wet granular is transferred to boiling-bed drying later
In, drying at 55 DEG C to moisture content is 2.0-4.0%, and the particle after drying is then crossed 10 mesh sieves.
The present invention is original with horse lyophilized powder of deer fetus, Radix Salviae Miltiorrhizae, pueraria lobata, Radix Paeoniae Alba, isoflavones when preparing health food
Material, red deer tire have been included in the medicine mesh that can be used for health food.Its nature and flavor is sweet, salty, temperature.Enter liver, kidney, the heart three to pass through.Function qi-restoratives is raw
Saliva.For deficient labor illness, asthenia of essence and blood, women's cold of insufficiency type, metrorrhagia and metrostaxis leukorrhagia.We take it, and qi-restoratives promotes the production of body fluid, and treat deficient labor illness, essence and blood
The function of deficiency.Its prominent qi-restoratives promotes the production of body fluid.Deficient labor illness is treated, the benefits of asthenia of essence and blood.Radix Salviae Miltiorrhizae, which has also been included in, can be used for health food
Among catalogue.Its is bitter in taste, slightly cold.Converge to heart and liver channels.Function stasis-dispelling and pain-killing, blood circulation, relieving restlessness and restlessness.For irregular menstruation,
Amenorrhea and algomenorrhea , lumps in the chest and abdomen, chest and abdomen shouting pain, hot numbness pain, sore and ulcer, swelling and pain, upset and sleepless;Hepatosplenomegaly, angina pectoris.In we
In the benefits of taking its blood circulation.Assist horse lyophilized powder of deer fetus regulating functional activities of vital QI, promoting blood circulation.It is both food and drug that pueraria lobata, which belongs to,
Article.Its nature and flavor is sweet, pungent, cool.Returns spleen, stomach meridian.Function expelling pathogenic factors from muscles and skin is brought down a fever, and is promoted the production of body fluid, promoting eruption, Shengyang Zhixie.With anti-oxidant, mention
The effect of high SOD activity intensity, scavenging activated oxygen, assistant reinforcement Eradicates chloasma.Radix Paeoniae Alba, which has been included in, can be used for health food
Among medicine mesh.Its is bitter in taste, acid, slightly cold.Return liver, the spleen channel.Function calming the liver and relieving pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating.It is dizzy for having a headache
It is dizzy, hypochondriac pain, abdominal pain, four limbs contraction pain, blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat.We take the benefits of its nourishing blood for regulating menstruation.Soybean is different
Flavones is medicine and healthy food material, is natural phytoestrogen, and we take its complementing estrogen, is delayed senescence, antioxygen
The effect of change, raising SOD activity intensity, scavenging activated oxygen, assistant reinforcement Eradicates chloasma.This product complete square compatibility is closed, and qi-restoratives promotes the production of body fluid,
Blood circulation, nourishing blood for regulating menstruation keep qi and blood, train of thought unobstructed, are aided with adjusting endocrine, remove free radical, anti peroxidation of lipid, improve
Microcirculation function.To reach the health-care efficacy of functions of removing chloasma.
Embodiment 2
The health food of functions of removing chloasma, calculates by weight, and active constituent includes:
240 parts of horse lyophilized powder of deer fetus, 1200 parts of Radix Salviae Miltiorrhizae, 1200 parts of pueraria lobata, 800 parts of Radix Paeoniae Alba and 25 parts of isoflavones.
It further include pharmaceutically acceptable auxiliary material in the health food of the functions of removing chloasma.
It in the health food of the functions of removing chloasma, calculates by weight, auxiliary material includes:
800 parts of dextrin, 125 parts of mannitol and 10 parts of sodium carboxymethylcellulose.
A kind of preparation method of the health food of functions of removing chloasma, comprising the following steps:
Step 1: by the Radix Salviae Miltiorrhizae of 1200 parts by weight, the pueraria lobata of 1200 parts by weight, 800 parts by weight Radix Paeoniae Alba volume fraction
It is extracted 2 times for 60% alcohol reflux, 2 hours every time, each solid-liquid ratio was 1:10g/mL, after merging filtrate, was in temperature
55-65 DEG C, negative pressure be -0.06~-0.08Mpa under be concentrated under reduced pressure, recycle ethyl alcohol after, temperature be 65-75 DEG C, negative pressure be -
Be concentrated under reduced pressure under 0.06~-0.08Mpa relative density be 1.25-1.30 (60 DEG C) when, obtain medicinal extract.
Step 2: dispensing medicinal extract into each stainless steel disc, it is put into 70 DEG C of baking oven, forced air drying 58h is dry to feel
Cream hardens, touching it is not viscous after take out, put to room temperature, left-hand thread stainless steel disc, tapping is tried to get to the heart of a matter to dry cream fall off after remove, collect dry
Cream crushes the medicinal extract after drying, crosses 80 meshes, obtains extract powder;
Step 3: remaining supplementary material is used 80 mesh sieves respectively, weigh later;Take 25 parts by weight isoflavones,
The sodium carboxymethylcellulose of 10 parts by weight is uniformly mixed, and is crossed 80 meshes, is obtained mixed powder I;Mixed powder I is mixed with the mannitol of equivalent again
It closes uniformly, crosses 80 meshes, obtain mixed powder II;Mixed powder II is uniformly mixed with remaining mannitol again, 80 meshes is crossed, must mix
Powder III;
Step 4: the dextrin of mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 240 parts by weight and 800 parts by weight is put into
20min is mixed in mixing machine, obtains total mixed powder, and it is total mixing silty amount that quality is added while stirring into mixing machine later
The ethyl alcohol that 25% volume fraction is 80%, stirs 10min after adding, and softwood is made;
Step 5: softwood is transferred to oscillating granulator, is pelletized with 16 meshes, wet granular is transferred to boiling-bed drying later
In, drying at 60 DEG C to moisture content is 2.0-4.0%, and the particle after drying is then crossed 14 mesh sieves.
Embodiment 3
The health food of functions of removing chloasma, calculates by weight, and active constituent includes:
280 parts of horse lyophilized powder of deer fetus, 1400 parts of Radix Salviae Miltiorrhizae, 1400 parts of pueraria lobata, 1000 parts of Radix Paeoniae Alba and 30 parts of isoflavones.
It further include pharmaceutically acceptable auxiliary material in the health food of the functions of removing chloasma.
It in the health food of the functions of removing chloasma, calculates by weight, auxiliary material includes:
1000 parts of dextrin, 130 parts of mannitol and 13 parts of sodium carboxymethylcellulose.
A kind of preparation method of the health food of functions of removing chloasma, comprising the following steps:
Step 1: by the Radix Salviae Miltiorrhizae of 1400 parts by weight, the pueraria lobata of 1400 parts by weight, 1000 parts by weight Radix Paeoniae Alba volume fraction
It is extracted 3 times for 70% alcohol reflux, 3 hours every time, each solid-liquid ratio was 1:15g/mL, after merging filtrate, was in temperature
55-65 DEG C, negative pressure be -0.06~-0.08Mpa under be concentrated under reduced pressure, recycle ethyl alcohol after, temperature be 65-75 DEG C, negative pressure be -
Be concentrated under reduced pressure under 0.06~-0.08Mpa relative density be 1.25-1.30 (60 DEG C) when, obtain medicinal extract;
Step 2: dispensing medicinal extract into each stainless steel disc, it is put into 75 DEG C of baking oven, forced air drying 60h is dry to feel
Cream hardens, touching it is not viscous after take out, put to room temperature, left-hand thread stainless steel disc, tapping is tried to get to the heart of a matter to dry cream fall off after remove, collect dry
Cream crushes the medicinal extract after drying, crosses 80 meshes, obtains extract powder;
Step 3: remaining supplementary material is used 120 mesh sieves respectively, weigh later;Take 30 parts by weight isoflavones,
The sodium carboxymethylcellulose of 13 parts by weight is uniformly mixed, and is crossed 80 meshes, is obtained mixed powder I;Mixed powder I is mixed with the mannitol of equivalent again
It closes uniformly, crosses 80 meshes, obtain mixed powder II;Mixed powder II is uniformly mixed with remaining mannitol again, 80 meshes is crossed, must mix
Powder III;
Step 4: the dextrin of mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 280 parts by weight and 1000 parts by weight is thrown
Enter and mix 40min in mixing machine, obtain total mixed powder, it is total mixing silty amount that quality is added while stirring into mixing machine later
The ethyl alcohol that 40% volume fraction is 80%, stirs 20min after adding, and softwood is made;
Step 5: softwood is transferred to oscillating granulator, is pelletized with 20 meshes, wet granular is transferred to boiling-bed drying later
In, drying at 65 DEG C to moisture content is 2.0-4.0%, and the particle after drying is then crossed 20 mesh sieves.
Embodiment 4
On the basis of embodiment 1, it in the health food of the functions of removing chloasma, calculates by weight, active constituent is also
It include: 25 parts of bamboo extractive, 10 parts of Flos Rosae Rugosae extract and 20 parts of Seabuckthorm Seed Oil;
It calculates by weight, auxiliary material further include: 30 parts of starch.
In the preparation method of the health food of the functions of removing chloasma, in the step 4, by mixed powder III, extract powder,
After mixing in the horse lyophilized powder of deer fetus of 200 parts by weight and the dextrin investment mixing machine of 600 parts by weight, then 25 are put into mixing machine
The starch of the bamboo extractive of parts by weight, the Flos Rosae Rugosae extract of 10 parts by weight and 30 parts by weight, mixes in mixing machine
20min obtains total mixed powder, sprays the ethyl alcohol that volume fraction is 80% in the form of atomization while stirring into mixing machine later
The mixed liquor formed with the Seabuckthorm Seed Oil of 20 parts by weight stirs 10min after having sprayed, softwood is made.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the bamboo extractive includes following
Step:
Step A, it by after fresh leaf of bamboo impurity elimination, sterilization, chopping, thaws at room temperature after the leaf of bamboo is freezed at -15 DEG C, weight
After multiple 2 times, the water for being 5 times of leaf of bamboo weight with yeast and weight that weight be leaf of bamboo weight 0.01% is uniformly mixed, at 25 DEG C
Lower sealing and fermenting 20h, obtains tunning one;Cell is put into freezing (about -15 DEG C) at low temperature, is then melted at room temperature,
It is repeated multiple times and reach certain broken wall effect.Due to freezing, the hydrophobic bond structure breaking of cell membrane on the one hand can be made, to increase
The hydrophilicity of refinement born of the same parents, on the other hand water crystallization intracellular, forms ice crystal grain, causes cell expansion and rupture.Freezing solution repeatedly
Material after jelly is destroyed because of eucaryotic cell structure is also beneficial to saccharomycetes to make fermentation, shortens fermentation time.Later stage fermentation can further be broken
Bad cell wall, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step B, the cellulase and weight that weight is one weight 0.5% of tunning are added into tunning one
For the pectase of one weight 0.5% of tunning, 1h is digested in the case where temperature is 40 DEG C, pH value is 5, filters later, obtains filtrate
One (for water-soluble extractive) and filter residue one;Cell wall is further destroyed by enzymatic hydrolysis, is easy to intracellular active principle
Dissolution.
Step C, spraying by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
It is dry, obtain powder one;After filter residue one is dry, crushing, 120 meshes are crossed, the alcohol reflux for being later 80% with volume fraction
2h, solid-liquid ratio are 1:10g/mL, after standing 10h, again with alcohol reflux 2 times that volume fraction is 80%, each solid-liquid ratio
It is 1:8g/mL, is 55-65 DEG C in temperature, negative pressure is under -0.06~-0.08Mpa after merging filtrate (ethanol-soluble extractives)
It is concentrated under reduced pressure, after recycling ethyl alcohol, spray drying obtains powder two;
Step D, powder one is mixed with powder two, obtains bamboo extractive.Water-soluble extractive and alcohol-soluble are mentioned
Object is taken to mix to get bamboo extractive.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the Flos Rosae Rugosae extract include with
Lower step:
Step 1), will after fresh roseleaf impurity elimination, sterilization, grinding, with weight be rose weight 0.01% ferment
The water that mother and weight are 5 times of rose weight is uniformly mixed, and is fermented 1 day at 25 DEG C, is obtained tunning two;Fermentation energy
Cell wall is destroyed, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step 2), into tunning two be added weight be two weight 0.5% of tunning cellulase, Yi Jichong
Amount is the pectase of two weight 0.5% of tunning, and 2h is digested in the case where temperature is 40 DEG C, pH value is 5, filters, is filtered later
Liquid two and filter residue two;Keep plant cell wall broken by enzymatic hydrolysis, rose tissue space and intracellular esters, alcohols etc. are fragrant
The ingredients such as effective component and the strong plant wax of adhesive force, protein, glucoside, carbohydrate are sufficiently freed.
Step 3), by filter residue two immerse weight be 10 times of two weight of filter residue 20 times of liquid of wood vinegar in, sealing and standing 10h
Afterwards, it flows back 1 time, after filtering, obtains filtrate three and filter residue three;Main ingredient in bamboo vinegar and wood vinegar is water, is followed by had
The substances such as machine acid, phenols, alcohols and ketone, thus also have the function of solvent.Vinegar liquid itself, which also has, removes free radical, antioxygen
The effect of change, that applies in the beverage is more.
Step 4), by filter residue three immerse weight be 7 times of three weight of filter residue 20 times of liquid of bamboo vinegar or wood vinegar in, sealing
1h is stood, after being dissolved in the active principle on filter residue three in bamboo vinegar or wood vinegar, assisting ultrasonic is extracted at being 30 DEG C in temperature
20min is filtered later, obtains filtrate four;
Step 5), by filtrate two, filtrate three and filtrate four mix after, temperature be 65-75 DEG C, negative pressure be -0.06~-
After being concentrated under reduced pressure under 0.08Mpa, spray drying obtains Flos Rosae Rugosae extract.
The present invention is when preparing health food, with horse lyophilized powder of deer fetus, Radix Salviae Miltiorrhizae, pueraria lobata, Radix Paeoniae Alba, isoflavones, the leaf of bamboo
Extract, Flos Rosae Rugosae extract and Seabuckthorm Seed Oil are raw material, and red deer tire has been included in the medicine mesh that can be used for health food.Its nature and flavor
It is sweet, salty, temperature.Enter liver, kidney, the heart three to pass through.Function qi-restoratives promotes the production of body fluid.For deficient labor illness, asthenia of essence and blood, women's cold of insufficiency type, metrorrhagia and metrostaxis leukorrhagia.
We take its qi-restoratives to promote the production of body fluid, and treat deficient labor illness, the function of asthenia of essence and blood.Its prominent qi-restoratives promotes the production of body fluid.Treat deficient labor illness, asthenia of essence and blood it
Effect.Radix Salviae Miltiorrhizae has also been included among the medicine mesh that can be used for health food.Its is bitter in taste, slightly cold.Converge to heart and liver channels.Function stasis-dispelling and pain-killing,
Blood circulation, relieving restlessness and restlessness.For irregular menstruation, amenorrhea and algomenorrhea , lumps in the chest and abdomen, chest and abdomen shouting pain, hot numbness pain, sore swell and ache curative,
Dysphoria and insomnia;Hepatosplenomegaly, angina pectoris.The benefits of its blood circulation is taken in we.Assist horse lyophilized powder of deer fetus regulating functional activities of vital QI, work
Blood and dissolving stasis.It is both food and the article of drug that pueraria lobata, which belongs to,.Its nature and flavor is sweet, pungent, cool.Returns spleen, stomach meridian.Function expelling pathogenic factors from muscles and skin is brought down a fever,
It promotes the production of body fluid, promoting eruption, Shengyang Zhixie.With anti-oxidant, improve SOD activity intensity, scavenging activated oxygen, assistant reinforcement Eradicates chloasma it
Effect.Radix Paeoniae Alba has been included among the medicine mesh that can be used for health food.Its is bitter in taste, acid, slightly cold.Return liver, the spleen channel.Function calming the liver is stopped
Bitterly, nourishing blood for regulating menstruation, astringing YIN to stop sweating.For headache and dizziness, hypochondriac pain, abdominal pain, four limbs contraction pain, blood deficiency chlorosis, irregular menstruation, spontaneous perspiration,
Night sweat.We take the benefits of its nourishing blood for regulating menstruation.Isoflavones is medicine and healthy food material, is natural phytoestrogen,
We take its complementing estrogen, delay senescence, anti-oxidant, improve SOD activity intensity, scavenging activated oxygen, assistant reinforcement Eradicates Huang
The effect of foxiness.Bamboo extractive effective ingredient includes flavones, phenolic acid, anthraquinone, lactones, polysaccharide, amino acid, microelement etc.,
With excellent free radical resisting, anti-oxidant, anti-aging, antifatigue, reducing blood lipid, prevention cardiovascular and cerebrovascular disease protection liver, expansion
Capillary, dredging microcirculation, activation brain promote memory, improve sleep, anticancer, beautification skin and other effects.Rose mentions
Take that physical property matter is mild, can chill out, balance endocrine, replenishing blood and vital energy, U.S. face skin care plays the role of conditioning to liver and stomach.Sea-buckthorn
Seed oil includes 140 various bioactive components such as flavones, organic acid, alkaloid, sterols, triterpene alkenes and various vitamins.
Because Seabuckthorm Seed Oil is rich in vitamin C and a large amount of antioxidants, thus it is beneficial to skin health.This product complete square compatibility is closed, and qi-restoratives promotes the production of body fluid,
Blood circulation, nourishing blood for regulating menstruation keep qi and blood, train of thought unobstructed, are aided with adjusting endocrine, remove free radical, anti peroxidation of lipid, improve
Microcirculation function.To reach the health-care efficacy of functions of removing chloasma.
Embodiment 5
It on the basis of embodiment 2, in the health food of the functions of removing chloasma, calculates by weight, active constituent is also
It include: 35 parts of bamboo extractive, 15 parts of Flos Rosae Rugosae extract and 25 parts of Seabuckthorm Seed Oil;
It calculates by weight, auxiliary material further include: 65 parts of starch.
In the preparation method of the health food of the functions of removing chloasma, in the step 4, by mixed powder III, extract powder,
After mixing in the horse lyophilized powder of deer fetus of 240 parts by weight and the dextrin investment mixing machine of 800 parts by weight, then 35 are put into mixing machine
The starch of the bamboo extractive of parts by weight, the Flos Rosae Rugosae extract of 15 parts by weight and 65 parts by weight, mixes in mixing machine
20min obtains total mixed powder, and spraying quality in the form of atomization while stirring into mixing machine later is total mixing silty amount
The mixed liquor of the ethyl alcohol that 25% volume fraction is 80% and the Seabuckthorm Seed Oil of 25 parts by weight composition stirs 10min after having sprayed,
Softwood is made.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the bamboo extractive includes following
Step:
Step A, it by after fresh leaf of bamboo impurity elimination, sterilization, chopping, thaws at room temperature after the leaf of bamboo is freezed at -15 DEG C, weight
After multiple 3 times, the water for being 7 times of leaf of bamboo weight with yeast and weight that weight be leaf of bamboo weight 0.05% is uniformly mixed, at 27 DEG C
Lower sealing and fermenting 25h, obtains tunning one;Cell is put into freezing (about -15 DEG C) at low temperature, is then melted at room temperature,
It is repeated multiple times and reach certain broken wall effect.Due to freezing, the hydrophobic bond structure breaking of cell membrane on the one hand can be made, to increase
The hydrophilicity of refinement born of the same parents, on the other hand water crystallization intracellular, forms ice crystal grain, causes cell expansion and rupture.Freezing solution repeatedly
Material after jelly is destroyed because of eucaryotic cell structure is also beneficial to saccharomycetes to make fermentation, shortens fermentation time.Later stage fermentation can further be broken
Bad cell wall, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step B, the cellulase and weight that weight is one weight 0.7% of tunning are added into tunning one
For the pectase of one weight 0.7% of tunning, 2h is digested in the case where temperature is 45 DEG C, pH value is 6, filters later, obtains filtrate
One (for water-soluble extractive) and filter residue one;Cell wall is further destroyed by enzymatic hydrolysis, is easy to intracellular active principle
Dissolution.
Step C, spraying by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
It is dry, obtain powder one;After filter residue one is dry, crushing, 120 meshes are crossed, the alcohol reflux 2 for being later 80% with volume fraction
Secondary, each 2.5h, each solid-liquid ratio is 1:10g/mL, after standing 11h, the alcohol reflux 3 for being again 80% with volume fraction
Secondary, each solid-liquid ratio is 1:8g/mL, is 55-65 DEG C in temperature, negative pressure is -0.06 after merging filtrate (ethanol-soluble extractives)
It is concentrated under reduced pressure under~-0.08Mpa, after recycling ethyl alcohol, spray drying obtains powder two;
Step D, powder one is mixed with powder two, obtains bamboo extractive.Water-soluble extractive and alcohol-soluble are mentioned
Object is taken to mix to get bamboo extractive.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the Flos Rosae Rugosae extract include with
Lower step:
Step 1), will after fresh roseleaf impurity elimination, sterilization, grinding, with weight be rose weight 0.05% ferment
The water that mother and weight are 7 times of rose weight is uniformly mixed, and is fermented 2 days at 27 DEG C, is obtained tunning two;Fermentation energy
Cell wall is destroyed, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step 2), into tunning two be added weight be two weight 0.5% of tunning cellulase, Yi Jichong
Amount is the pectase of two weight 0.7% of tunning, and 3h is digested in the case where temperature is 45 DEG C, pH value is 6, filters, is filtered later
Liquid two and filter residue two;Keep plant cell wall broken by enzymatic hydrolysis, rose tissue space and intracellular esters, alcohols etc. are fragrant
The ingredients such as effective component and the strong plant wax of adhesive force, protein, glucoside, carbohydrate are sufficiently freed.
Step 3), by filter residue two immerse weight be 12 times of two weight of filter residue 35 times of liquid of bamboo vinegar or wood vinegar in, sealing
After standing 11h, flows back 1 time, after filtering, obtain filtrate three and filter residue three;Main ingredient in bamboo vinegar and wood vinegar is water,
Secondary is the substances such as organic acid, phenols, alcohols and ketone, thus also has the function of solvent.Vinegar liquid itself, which also has, to be removed freely
Base, oxidation resistant effect, that applies in the beverage is more.
Step 4), by filter residue three immerse weight be 8 times of three weight of filter residue 35 times of liquid of wood vinegar in, sealing and standing 2h makes
After active principle on filter residue three is dissolved in bamboo vinegar or wood vinegar, assisting ultrasonic extracts 25min at being 35 DEG C in temperature, later
Filtering, obtains filtrate four;
Step 5), by filtrate two, filtrate three and filtrate four mix after, temperature be 65-75 DEG C, negative pressure be -0.06~-
After being concentrated under reduced pressure under 0.08Mpa, spray drying obtains Flos Rosae Rugosae extract.
Embodiment 6
It on the basis of embodiment 3, in the health food of the functions of removing chloasma, calculates by weight, active constituent is also
It include: 45 parts of bamboo extractive, 20 parts of Flos Rosae Rugosae extract and 30 parts of Seabuckthorm Seed Oil;
It calculates by weight, auxiliary material further include: 100 parts of starch.
In the preparation method of the health food of the functions of removing chloasma, in the step 4, by mixed powder III, extract powder,
The horse lyophilized powder of deer fetus of 280 parts by weight and the dextrin of 1000 parts by weight are put into mixing machine after mixing, then are put into mixing machine
The starch of the bamboo extractive of 45 parts by weight, the Flos Rosae Rugosae extract of 20 parts by weight and 100 parts by weight, mixes in mixing machine
20min obtains total mixed powder, and spraying quality in the form of atomization while stirring into mixing machine later is total mixing silty amount
The mixed liquor of the ethyl alcohol that 40% volume fraction is 80% and the Seabuckthorm Seed Oil of 30 parts by weight composition stirs 10min after having sprayed,
Softwood is made.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the bamboo extractive includes following
Step:
Step A, it by after fresh leaf of bamboo impurity elimination, sterilization, chopping, thaws at room temperature after the leaf of bamboo is freezed at -15 DEG C, weight
After multiple 3 times, the water for being 10 times of leaf of bamboo weight with yeast and weight that weight be leaf of bamboo weight 0.1% is uniformly mixed, at 30 DEG C
Lower sealing and fermenting 30h, obtains tunning one;Cell is put into freezing (about -15 DEG C) at low temperature, is then melted at room temperature,
It is repeated multiple times and reach certain broken wall effect.Due to freezing, the hydrophobic bond structure breaking of cell membrane on the one hand can be made, to increase
The hydrophilicity of refinement born of the same parents, on the other hand water crystallization intracellular, forms ice crystal grain, causes cell expansion and rupture.Freezing solution repeatedly
Material after jelly is destroyed because of eucaryotic cell structure is also beneficial to saccharomycetes to make fermentation, shortens fermentation time.Later stage fermentation can further be broken
Bad cell wall, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step B, into tunning one, addition weight is the cellulase of one weight 1% of tunning and weight is
The pectase of one weight 1% of tunning, in the case where temperature is 50 DEG C, pH value is 6 digest 2h, filter later, obtain filtrate one (for
Water-soluble extractive) and filter residue one;Cell wall is further destroyed by enzymatic hydrolysis, intracellular active principle is made to be easy to dissolve out.
Step C, spraying by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
It is dry, obtain powder one;After filter residue one is dry, crushing, 150 meshes are crossed, the alcohol reflux 2 for being later 80% with volume fraction
Secondary, each 3h, each solid-liquid ratio is 1:10g/mL, and after standing 12h, using volume fraction again is alcohol reflux 3 times of 80%,
Each solid-liquid ratio is 1:8g/mL, is 55-65 DEG C in temperature after merging filtrate (ethanol-soluble extractives), and negative pressure is -0.06~-
It is concentrated under reduced pressure under 0.08Mpa, after recycling ethyl alcohol, spray drying obtains powder two;
Step D, powder one is mixed with powder two, obtains bamboo extractive.Water-soluble extractive and alcohol-soluble are mentioned
Object is taken to mix to get bamboo extractive.
In the preparation method of the health food of the functions of removing chloasma, the preparation method of the Flos Rosae Rugosae extract include with
Lower step:
Step 1), will after fresh roseleaf impurity elimination, sterilization, grinding, with weight be rose weight 0.1% yeast,
And the water that weight is 10 times of rose weight is uniformly mixed, and is fermented 2 days at 30 DEG C, is obtained tunning two;Fermentation energy is broken
Bad cell wall, and macromolecular substances are resolved into the small-molecule substance being easily absorbed by the human body.
Step 2), into tunning two be added weight be two weight 1% of tunning cellulase and weight
For the pectase of two weight 1% of tunning, 3h is digested in the case where temperature is 50 DEG C, pH value is 6, filters later, obtains filtrate two
With filter residue two;Keep plant cell wall broken by enzymatic hydrolysis, the fragrance such as rose tissue space and intracellular esters, alcohols is effectively
The ingredients such as ingredient and the strong plant wax of adhesive force, protein, glucoside, carbohydrate are sufficiently freed.
Step 3), by filter residue two immerse weight be 15 times of two weight of filter residue 50 times of liquid of bamboo vinegar in, sealing and standing 12h
Afterwards, it flows back 1 time, after filtering, obtains filtrate three and filter residue three;Main ingredient in bamboo vinegar and wood vinegar is water, is followed by had
The substances such as machine acid, phenols, alcohols and ketone, thus also have the function of solvent.Vinegar liquid itself, which also has, removes free radical, antioxygen
The effect of change, that applies in the beverage is more.
Step 4), by filter residue three immerse weight be 10 times of three weight of filter residue 50 times of liquid of bamboo vinegar in, sealing and standing 2h,
After being dissolved in the active principle on filter residue three in bamboo vinegar or wood vinegar, assisting ultrasonic extracts 30min at being 40 DEG C in temperature, it
After filter, obtain filtrate four;
Step 5), by filtrate two, filtrate three and filtrate four mix after, temperature be 65-75 DEG C, negative pressure be -0.06~
After being concentrated under reduced pressure under -0.08Mpa, spray drying obtains Flos Rosae Rugosae extract.
Comparative example 1
The raw materials used component of the health food of functions of removing chloasma and dosage are the same as embodiment 2.
The preparation method of the health food of functions of removing chloasma, comprising the following steps:
Step 1: the Radix Paeoniae Alba of the Radix Salviae Miltiorrhizae of 1200 parts of parts by weight, the pueraria lobata of 1200 parts of parts by weight, 800 parts of parts by weight is distinguished
It is mixed after grinding, obtains mixed powder A;
Step 2: remaining supplementary material is used 80 mesh sieves respectively, weigh later;By the isoflavones of 25 parts by weight,
The sodium carboxymethylcellulose of 10 parts by weight is uniformly mixed, and is crossed 80 meshes, is obtained mixed powder I;Mixed powder I is mixed with the mannitol of equivalent again
It closes uniformly, crosses 80 meshes, obtain mixed powder II;Mixed powder II is uniformly mixed with remaining mannitol again, 80 meshes is crossed, must mix
Powder III;
Step 3: the dextrin of mixed powder III, mixed powder A, the horse lyophilized powder of deer fetus of 240 parts by weight and 800 parts by weight is thrown
Enter and mix 20min in mixing machine, obtain total mixed powder, it is total mixing silty amount that quality is added while stirring into mixing machine later
The ethyl alcohol that 25% volume fraction is 80%, stirs 10min after adding, and softwood is made;
Step 4: softwood is transferred to oscillating granulator, is pelletized with 16 meshes, wet granular is transferred to boiling-bed drying later
In, drying at 55-65 DEG C to moisture content is 2.0-4.0%, and the particle after drying is then crossed 14 mesh sieves.
Comparative example 2
The preparation method of the health food of functions of removing chloasma compared with Example 5, the component of used raw material and each component
Dosage is identical, and only bamboo extractive is different with the preparation method of Flos Rosae Rugosae extract.
(1) bamboo extractive preparation method the following steps are included:
Step A, the cellulase that weight is leaf of bamboo weight 0.7% after fresh leaf of bamboo impurity elimination, sterilization, chopping, will be added, with
And weight is the pectase of leaf of bamboo weight 0.7%, and 2h is digested in the case where temperature is 45 DEG C, pH value is 6, filters later, obtains filtrate
One and filter residue one;
Step B, spraying by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
It is dry, obtain powder one;After filter residue one is dry, crushing, 120 meshes are crossed, the alcohol reflux 2 for being later 80% with volume fraction
Secondary, each 2.5h, each solid-liquid ratio is 1:10g/mL, after standing 11h, the alcohol reflux 3 for being again 80% with volume fraction
Secondary, each solid-liquid ratio is 1:8g/mL, is 55-65 DEG C in temperature, negative pressure is to subtract under -0.06~-0.08Mpa after merging filtrate
Pressure concentration, after recycling ethyl alcohol, spray drying obtains powder two;
Step C, powder one is mixed with powder two, obtains bamboo extractive.
(2) Flos Rosae Rugosae extract preparation method the following steps are included:
Step a, by after the roseleaf cleaning of picking, sterilization, grinding obtains slurries;
Step b, into slurries, addition weight is the cellulase of slurry weight 0.5% and weight is slurry weight 0.7%
Pectase, in the case where temperature is 45 DEG C, pH value is 6 digest 3h, obtain enzymolysis liquid;
Step c, it by after enzymolysis liquid filtering, enzyme deactivation, is depressurized in the case where temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa
After concentration, spray drying obtains Flos Rosae Rugosae extract.
Test 1
Pass through general flavone content (%), the extract of bamboo extractive in spectrophotometry testing example 5 and comparative example 2
Yield (%), extract yield=m '/m, m ' are extract weight, and m is leaf of bamboo weight, and test result is shown in Table 1.
General flavone content and extract yield in bamboo extractive in 1 embodiment 5 of table and comparative example 2
As shown in Table 1, total yellow in the bamboo extractive of preparation using the method for embodiment 5 compared with the method for comparative example 2
Ketone content and extract yield are higher, illustrate to significantly improve always using the method provided by the present application for preparing bamboo extractive
Flavones content and extract yield.
Test 2
Test is using Determination of Polyphenols (%), extraction in the Flos Rosae Rugosae extract of the method for embodiment 5 and comparative example 2 preparation
Object yield (%), extract yield=m '/m, m ' are extract weight, and m is rose weight, and test result is shown in Table 2.
Determination of Polyphenols and extract yield in Flos Rosae Rugosae extract in 2 embodiment 5 of table and comparative example 2
Project | Determination of Polyphenols (%) | Extract yield (%) |
Embodiment 5 | 79 | 20 |
Comparative example 2 | 51 | 15 |
As shown in Table 2, using Determination of Polyphenols and extract yield in the Flos Rosae Rugosae extract of the method preparation of embodiment 5
It is higher.
Test 3
1 object and method
1.1 tested materials: the particle prepared in embodiment 2,5 and comparative example 1,2.Human body recommended amounts are 2 times a day every time 1
Bag, dose are 10g/ days.
1.2 study subjects: being selected 23-60 years old by the principle of voluntariness, has chloasma subject 250.
1.2.1 diagnostic criteria: Head And Face visually observes visible brown spot, is mainly in cheekbone, cheek, volume, nose, lip week etc.,
The symptoms such as no inflammation, sufferings.
1.2.2 be not included in person's standard: all volunteers for having chloasma can carry out test-meal observation through medical fitness.
1.2.3 eliminator's standard:
1.2.3.1 the age is in under-18s or over-65s person, pregnant or breast feeding women, those who are allergic to the health care products.
1.2.3.2 the severe primaries disease such as conscience kidney and hemopoietic system, mental patient are associated with.
1.2.3.3 the standard of being included in is not met, not by regulation tested material, can not determining effect or data, umbra does not ring effect
Or safety judgement person.
1.3 experimental designs are with grouping: using and compare two kinds of forms between itself cross-reference and group.Subject is randomly divided into examination
Food group and control group, test-meal group take tested material, and control group is blank control.
1.4 taking doses and time: 1 bag every time, 2 times a day, dose is 10g days, and continuous 30 days, subject was in test-meal
Period cuts out in relation to the drug of face-beautifying and spot-removing or other health care products and cosmetics.
1.5 instruments and reagent: CA-500 type blood counting instrument (Japan produces), BM200 urinates ten analyzers (U.S.'s production),
AUTOLAB automatic clinical chemistry analyzer (Italy produces), Institute of Geography, Academia Sinica's development and design maps out
The publication " practical standard colour atla " (first edition) in 1992 of version society, biochemical reagents box are all provided by Zhong Sheng company.
2 observation index:
Following observation index experiment front and back is each to be checked once.
2.1 efficiency observations:
2.1.1 ordinary circumstance:
Detailed medical history-taking, understands subject's diet situation, and observation sleep, stool and urine, mental status, blood pressure etc. mainly face
Bed symptom.Whether observation tested material has adverse effect to subject.
2.1.2 Head And Face chloasma detects:
2.1.2.1 size changes
With the long diameter and wide diameter of the same chloasma in the tested front and back of tape measure, the area of entire facial area chloasma is calculated
(cm2)
2.1.2.2 shade changes:
According to Institute of Geography, Academia Sinica's development and design, Mapping Press's " practical standard colour atla " published in 1992
Brown (Y+M+Bk is yellow+pinkish red+black folded color) colour atla in (the edition) is the judgment criteria of the chloasma color depth: 1 degree
(15,20,5), II degree (30,40,10), III degree (40,60,15).
2.2 safety observations:
2.2.1 blood and routine urianlysis:
Red blood cell count(RBC), hemoglobin, white blood cell count(WBC) urinate ten, just conventional detection.
2.2 biochemical row's mappings are fixed:
Seralbumin ALB, total protein TP, hepatic and renal function (total bilirubin TBIL, glutamic-pyruvic transaminase ALT, urea nitrogen BUN,
Inosine CRE), blood glucose GLU, blood lipid (total cholesterol TC, triglyceride TG)
2.2.3 abdominal B-scan ultrasonography, electrocardiogram, x-ray fluoroscopy of chest.
2.3 spot-removal functions determine:
Effective: chloasma color declines I degree, and area, which is reduced, is greater than 10%, and does not generate new chloasma.
Invalid: chloasma color and area are without significant change.
2.4 data statistic analysis:
Experimental data is measurement data, can be examined and be analyzed with t.All own control data can use paired t-test,
Two groups of means compare using independent samples t-test, and the latter need to carry out homogeneity test of variance, the data not risen to Non-Gaussian Distribution or variance
Variable conversion appropriate is carried out, after meeting normal state variance, carries out t inspection with the data of conversion.
Effective percentage uses X2Inspection is tested.Four fold table total number of cases are less than 40 or total number of cases are equal to or more than 40 but go out
When existing theoretical value is equal to or less than 1, Ying Gaiwei exact propability.
2.5 result judgements:
Test-meal group area of chloasma is averagely reduced, and is more than or equal to 10%, and color integral is decreased obviously, itself front and back is compared
And compared with the control group, difference has conspicuousness, and does not generate new chloasma, can determine that the given the test agent has functions of removing chloasma
Function.
3 experimental results
3.1 general status:
Subject is randomly divided into experimental group and control group, totally 250 effectively, be women.Test-meal group 200, the age is most
It is 23 years old small, it is 62 years old, average age 48.26 ± 7.63 years old maximum;Control group 50, the age is 24 years old minimum, 60 years old maximum, average year
Age 46.90 ± 10.94 years old.
The variation of the 3.2 chloasma color depths:
Chloasma color weight variation (X ± SD) before and after 3 test-meal of table
* itself compares P < 0.01, ## comparison among groups P < 0.01
3.3 area of chloasma size variations
Area of chloasma size variation (X ± SD) before and after 4 test-meal of table
* itself compare the # comparison among groups ## comparison among groups of P < 0.05 of P < 0.05 P < 0.01
3.4 effect judges:
Effect judge result before and after 5 test-meal of table
# comparison among groups P < 0.05
By table 3,4,5 as it can be seen that after functions of removing chloasma health-care product particle prepared by test-meal group test-meal embodiment 2, test-meal group is yellowish-brown
Spot lighter, colour atla averagely reduce by 0.38 ± 0.37 degree, and area of chloasma averagely reduces 5.58 ± 3.42cm2, test-meal group
After functions of removing chloasma health-care product particle prepared by test-meal embodiment 5, test-meal group chloasma color shoals, and colour atla averagely reduces by 0.63 ±
0.23 degree, area of chloasma averagely reduces 6.73 ± 3.87cm2, and colour atla, area of chloasma are without bright before and after control group test-meal
Aobvious variation.After functions of removing chloasma health-care product particle prepared by test-meal group test-meal embodiment 2 compared with the control group, chloasma color shoals
Have significant difference (P < 0.05) with area of chloasma, before functions of removing chloasma health-care product particle prepared by test-meal group test-meal embodiment 2
Chloasma color shoals afterwards, area reduces and has significant difference (P < 0.05), total effective rate 16.0%, is higher than control
Group (P < 0.05).After functions of removing chloasma health-care product particle prepared by test-meal group test-meal embodiment 5 compared with the control group, chloasma color
Shoaling has significant difference (P < 0.05) with area of chloasma, functions of removing chloasma health care product prepared by test-meal group test-meal embodiment 5
Grain front and back chloasma color shoals, area reduces and has significant difference (P < 0.05), and total effective rate 30.0% is higher than
Control group (P < 0.05).
3.5 ordinary circumstance
Ordinary circumstance analysis before and after the functions of removing chloasma health-care product particle of 6 test-meal embodiment 2 of table preparation
Cardinal symptom | Number of cases | Effectively | In vain | Total effective rate |
Diet | 0(0) | 0(0) | 0(0) | 0(0) |
Stool and urine | 11(9) | 4(1) | 7(8) | 36.4% (11.1%) |
Sleep | 24(16) | 11(1) | 13(15) | 45.8% (6.2%) |
Mental status | 0(0) | 0(0) | 0(0) | 0(0) |
It is control group in ()
By table 6 as it can be seen that tested material has no adverse effects to the ordinary circumstance of subject after test-meal.
Ordinary circumstance analysis before and after the functions of removing chloasma health-care product particle of 7 test-meal embodiment 5 of table preparation
Cardinal symptom | Number of cases | Effectively | In vain | Total effective rate |
Diet | 0(0) | 0(0) | 0(0) | 0(0) |
Stool and urine | 11(9) | 6(1) | 5(8) | 54.5% (11.1%) |
Sleep | 24(16) | 14(1) | 10(15) | 58.3% (6.25%) |
Mental status | 0(0) | 0(0) | 0(0) | 0(0) |
It is control group in ()
By table 7 as it can be seen that tested material has no adverse effects to the ordinary circumstance of subject after test-meal.
3.6 blood routines and blood biochemistry variation:
Blood routine and blood biochemistry testing result before and after the functions of removing chloasma health-care product particle of 8 test-meal embodiment 2,5 of table preparation
By table 8 as it can be seen that test-meal group and control group items blood biochemistry and routine blood indexes are in normal range (NR) before and after test-meal.
3.7 urine and just conventional variation
Blood pressure and urine, feces conventional detection result before and after the functions of removing chloasma health-care product particle of 9 test-meal embodiment 2,5 of table preparation
By table 9 as it can be seen that test-meal group and control group blood pressure and urine, feces conventional index are in normal range (NR) before and after test-meal.
The functions of removing chloasma health-care product particle postabdomen B ultrasound of 3.8 test-meal embodiments 2,5 preparation, electrocardiogram, x-ray fluoroscopy of chest
Test-meal group and control group are in normal range (NR) before and after test-meal.
4 conclusions:
4.1 use itself two kinds of control design between group, and effective case 250, test-meal group (embodiment 2), test-meal group are (real
Apply example 5), test-meal group (comparative example 1), test-meal group (comparative example 2), control group be respectively 50.Test-meal group takes embodiment 2 as required
Behind particle 30 days of preparation, area of chloasma averagely reduces 5.58 ± 3.42cm2(P < 0.05 compared with the control group), area is average
It is reduced to 15.30% ± 9.17%;Lighter, colour atla averagely reduce by 0.38 ± 0.37 degree (P < 005 compared with the control group);Examination
Food group effective 8, total effective rate 16.00%.Compare before and after test-meal group itself and control group comparison among groups color integrates and area
Variation has conspicuousness (P < 0.05).It is yellowish-brown according to dispelling in " health food is examined and assessment technique specification " (version in 2003)
Spot function criterion, it is believed that particle prepared by embodiment 2 plays the role of functions of removing chloasma.Test-meal group takes embodiment 5 as required
Behind particle 30 days of preparation, area of chloasma averagely reduces 6.73 ± 3.87cm2(P < 0.05 compared with the control group), area is average
It is reduced to 18.50% ± 8.35%;Lighter, colour atla averagely reduce by 0.63 ± 0.23 degree (P < 005 compared with the control group);Examination
Food group effective 15, total effective rate 30.00%.Compare before and after test-meal group itself and control group comparison among groups color integrates and face
Product variation has conspicuousness (P < 0.05).According to Huang of dispelling in " health food is examined and assessment technique specification " (version in 2003)
Foxiness function criterion, it is believed that particle prepared by embodiment 5 plays the role of functions of removing chloasma.
Before and after the particle test-meal of 4.2 embodiments 2,5 preparation, blood routine, routine urinalysis, just routine and blood biochemistry index are just
Normal range, the particle for illustrating prepared by embodiment 2,5 have no adverse effects to subject's health.
After the particle test-meal of 4.3 embodiments 2,5 preparation, allergy and other adverse reactions are had no.
Test 4
1. test objective understands short-term oral contact tested material to the toxic effect and possible target organ of rat.
2. materials and methods
2.1. tested material: the particle prepared in embodiment 2,5, the human body recommended amounts (people of sample after processing that is 10g/ person/days
Body recommended amounts are equivalent to 6g/ person/days).
2.2. for sample pre-treatments in 30 days feeding trials of rat, additive amount of the sample in feed is no more than 10%, because
This reduces the dextrin auxiliary material (every packet reduces 2g dextrin) in sample under the premise of keeping effective component total amount constant, makes its offer
The human body recommended amounts of test specimen are equivalent to 6g/ person/days.Test specimen is taken according to dosage uniformly to admix respectively with the conversion of weight 10%
Basal feed.
2.3. the cleaning grade that experimental animal selects Zhejiang Province's Experimental Animal Center to provide is healthy, weans, the SD of weight 70-90g
Rat 140, half male and half female.Medical experiment animal certificate number SCXK (Zhejiang) 2003-0001, medical experiment animal facility environment
Quality certification number SYXK (Zhejiang) 2005-0074.
2.4. 20~25 DEG C of animal feeding ambient room temperature, relative humidity 60~70%, single cage raising.
2.5. basal feed is provided by Zhejiang Province's Experimental Animal Center.
2.6. test method
2.6.1. tested material mode oral administration is given, admixes feed, ad lib by various dose requirement.
2.6.2. dose design is set with the 25 of human body recommended amounts, 50,100 times as basic, normal, high three dosage groups, that is, is implemented
The particle prepared in example 2,5 distinguishes 2.5, three 5.0,10.0g/kg weight dosage groups, separately sets a negative control group.
2.6.3. operating procedure
2.6.3.1. sample preparation
2.5g/kg weight group: 25g sample is incorporated into the mixing of 975g basal feed, feed total amount 24000g;
5.0gkg weight group: 50g sample is incorporated into the mixing of 950g basal feed, feed total amount 24000g;
10.0gkg weight group: 100g sample and add 18g casein be incorporated into 882g basal feed mixing, feed total amount
24000g。
2.6.3.2. animal packet and test index
Experimental rat is randomly divided into 7 groups by weight, and every group 20, half male and half female.Experimental rat ad lib, drinking-water, see
Situations such as examining and recording general performance, behavior, poisoning symptom and the death of animal.Weight and food-intake are recorded weekly and calculate food
Object utilization rate.End of term overnight fasting is tested, eyeball is plucked and takes blood, sacrificed by decapitation carries out internal organs gross examination of skeletal muscle to every rat, and takes
Liver, kidney, spleen, testis weigh and calculate dirty body ratio, while carrying out blood routine, blood bio-chemistry checking, leave and take liver, kidney,
Spleen, Stomach duodenum, testis and ovary carry out pathologic examination.Blood routine uses Abacus automated blood analysis
Instrument is measured, including the indexs such as red blood cell, leucocyte, blood platelet, content of hemoglobin and the classification of leucocyte three.Blood biochemistry inspection
Look into and serum taken to be measured with Liasys automatic clinical chemistry analyzer, including glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST),
Total cholesterol (Chol), triglyceride (TG), creatinine (CR), urea nitrogen (Urea), total protein (TP), albumin (ALB), blood glucose
(Glu) indexs such as.Internal organs paraffin section, H-E dyeing.
2.7. data processing calculates the average and standard deviation of each observation index, and carries out t inspection
3. experimental result
3.1. overview: during experiment, each dosage group experimental rat no abnormality seen sings and symptoms, also without death.
3.2. 10 influence of the particle prepared in embodiment 2,5 to experimental rat weight: be the results are shown in Table.
Each dosage group female and the body weight increase of male experimental rat are shown slightly slowly, and Zhou Tichong mean value is with experiment progress and yin
Property control group gap be gradually increased, but with negative control group ratio, through statistics there are no significant difference.
The influence of the granules in rats weight (g) prepared in 10 embodiment 2,5 of table
3.3. 11 influence of the particle prepared in embodiment 2,5 to experimental rat food-intake: be the results are shown in Table.
The food-intake weekly and total food-intake of each dosage group female and male experimental rat are close with negative control group, with yin
Property control group than there are no significant difference.
The influence of the food-intake (g) weekly of the granules in rats prepared in 11 embodiment 2,5 of table
3.4. influence of the particle prepared in embodiment 2,5 to experimental rat food utilization, the results are shown in Table 12.
It is negative right that the food utilization weekly and total food utilization rate of each dosage group female and male experimental rat are slightly below
According to group, and negative control group ratio, through statistics there are no significant difference.
The influence of the granules in rats prepared in 12 embodiment 2,5 of table food utilization (%) weekly
3.5. 13 influence of the particle prepared in embodiment 2,5 to experimental rat blood routine: be the results are shown in Table.
The hemoglobin of female and each dosage group of male experimental rat, red blood cell count(RBC), white blood cell count(WBC) and classification exist
Normal range (NR).The particle prepared in embodiment 2,5 is to experimental rat hemoglobin, red blood cell count(RBC), white blood cell count(WBC) and classification
It has no and significantly affects.
The influence of the granules in rats blood routine prepared in 13 embodiment 2,5 of table
3.6. influence of the particle prepared in embodiment 2,5 to experimental rat blood biochemistry: it the results are shown in Table 14, table 15.
The blood parameters of control group and each dosage group female and male experimental rat are in normal range (NR).Embodiment 2,
The particle prepared in 5 has no experimental rat blood biochemistry and significantly affects.
The influence of the granules in rats blood biochemistry prepared in 14 embodiment 2,5 of table
Influence of the particle prepared in 15 embodiment 2,5 of table to experimental rat blood biochemistry
3.7. influence of the particle prepared in embodiment 2,5 to experimental rat dirty body ratio: it the results are shown in Table 16, table 17.
The dirty body ratio of each dosage group female and male experimental rat is close with negative control group, no significant difference.Implement
The particle prepared in example 2,5 has no significant effect the dirty body ratio of experimental rat.
3.8. pathologic examination result:
Experimental rat gross anatomy observation result is showed no abnormal change.Pathological section microscopy discovery control group has 1 hero
The property individual central veins of liver mild hyperaemias of rat liver;Control group have 2, high dose group have 3 rat kidney locally individual kidneys
Interstitial capillary mild hyperaemia;Spleen, ovary, testis, stomach, intestines are showed no abnormal change.The above variation control group and high agent
Amount group compares abiology meaning.The particle prepared in embodiment 2,5 to experimental rat gross anatomy, histological findings not
See and significantly affects.
The influence of the dirty body ratio of granules in rats prepared in 16 embodiment 2,5 of table
The influence of the dirty body ratio of granules in rats prepared in 17 embodiment 2,5 of table
4. conclusion
30 days feeding trials of rat of the particle prepared in embodiment 2,5, have no that poisoning symptom occurs in experimental animal, substantially
Histological anatorny observes also no abnormality seen pathological change, to rat body weight, food-intake, food utilization, blood routine, blood biochemistry
It all has no significant effect.30 days feeding trials of rat of the particle prepared in this EXPERIMENTAL EXAMPLE 2,5 do not observe maximum nocuousness
Dosages are 10.0g/kg weight.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (9)
1. the health food of functions of removing chloasma, which is characterized in that calculate by weight, active constituent includes:
200-280 parts of horse lyophilized powder of deer fetus, 1000-1400 parts of Radix Salviae Miltiorrhizae, 1000-1400 parts of pueraria lobata, 600-1000 parts of Radix Paeoniae Alba and big
20-30 parts of beans isoflavones.
2. the health food of functions of removing chloasma as described in claim 1, which is characterized in that further include pharmaceutically acceptable it is auxiliary
Material.
3. the health food of functions of removing chloasma as claimed in claim 2, which is characterized in that calculate by weight, auxiliary material includes:
600-1000 parts of dextrin, 110-130 parts of mannitol and 7-13 parts of sodium carboxymethylcellulose.
4. the health food of functions of removing chloasma as claimed in claim 3, which is characterized in that calculate by weight, active constituent is also
It include: 25-45 parts of bamboo extractive, 10-20 parts of Flos Rosae Rugosae extract and 20-30 parts of Seabuckthorm Seed Oil;
It calculates by weight, auxiliary material further include: 30-100 parts of starch.
5. the preparation method of the health food of functions of removing chloasma as claimed in claim 3, which comprises the following steps:
Step 1: by the Radix Salviae Miltiorrhizae of 1000-1400 parts by weight, the pueraria lobata of 1000-1400 parts by weight, 600-1000 parts by weight Radix Paeoniae Alba
It extracting 1-3 times with the alcohol reflux that volume fraction is 50-70%, 1-3 hours each, each solid-liquid ratio is 1:8-15g/mL,
It is 55-65 DEG C in temperature, negative pressure is to be concentrated under reduced pressure under -0.06~-0.08Mpa, after recycling ethyl alcohol, then in temperature after merging filtrate
Degree is 65-75 DEG C, when negative pressure is that be concentrated under reduced pressure into relative density under -0.06~-0.08Mpa be 1.25-1.30, obtains medicinal extract;
Step 2: by medicinal extract be 65-75 DEG C in temperature at it is dry after, put to room temperature, later crush dry cream, cross 80 meshes, obtain
Extract powder;
Step 3: remaining supplementary material is used 60-120 mesh sieve respectively, weigh later;Take the different Huang of the soybean of 20-30 parts by weight
Ketone, the sodium carboxymethylcellulose of 7-13 parts by weight are uniformly mixed, cross 60-80 mesh, obtain mixed powder I;Mixed powder I again with equivalent
Mannitol is uniformly mixed, and is crossed 60-80 mesh, is obtained mixed powder II;Mixed powder II is uniformly mixed with remaining mannitol again, mistake
60-80 mesh obtains mixed powder III;
Step 4: by the paste of mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 200-280 parts by weight and 600-1000 parts by weight
20-40min is mixed in essence investment mixing machine, obtains total mixed powder, it is total mix that quality is added while stirring into mixing machine later
The ethyl alcohol that the volume fraction of silty amount 10-40% is 80% is closed, stirs 10-20min after adding, softwood is made;
Step 5: softwood is transferred to oscillating granulator, is pelletized with 10-20 mesh, wet granular is transferred to boiling-bed drying later
In, drying at 55-65 DEG C to moisture content is 2.0-4.0%, and the particle after drying is then crossed 10-20 mesh sieve.
6. the preparation method of the health food of functions of removing chloasma as claimed in claim 5, which is characterized in that in the step 4,
The dextrin of mixed powder III, extract powder, the horse lyophilized powder of deer fetus of 200-280 parts by weight and 600-1000 parts by weight is put into mixing machine
After middle mixing, then put into mixing machine the bamboo extractives of 25-45 parts by weight, 10-20 parts by weight Flos Rosae Rugosae extract and
The starch of 30-100 parts by weight, mixes 20-40min in mixing machine, obtains total mixed powder, while stirring into mixing machine later
It is the ethyl alcohol and 20-30 parts by weight that total volume fraction for mixing silty amount 10-40% is 80% that quality is sprayed in the form of atomization
Seabuckthorm Seed Oil composition mixed liquor, 10-20min, obtained softwood are stirred after spray.
7. the preparation method of the health food of functions of removing chloasma as claimed in claim 6, which is characterized in that the bamboo extractive
Preparation method the following steps are included:
Step A, it by after fresh leaf of bamboo impurity elimination, sterilization, chopping, thaws at room temperature after the leaf of bamboo is freezed at -15 DEG C, repeats 2-
After 3 times, it is uniformly mixed with water that yeast and weight that weight is leaf of bamboo weight 0.01-0.1% are 5-10 times of leaf of bamboo weight,
It is sealed by fermentation 20-30h at 25-30 DEG C, obtains tunning one;
Step B, into tunning one, addition weight is the cellulase of one weight 0.5-1% of tunning and weight is
The pectase of one weight 0.5-1% of tunning digests 1-2h in the case where temperature is 40-50 DEG C, pH value is 5-6, filters, obtain later
To filtrate one and filter residue one;
Step C, by filtrate one after being concentrated under reduced pressure under temperature is 65-75 DEG C, negative pressure is -0.06~-0.08Mpa, spray drying,
Obtain powder one;After filter residue one is dry, crushing, 100-150 mesh is crossed, is mentioned later with the alcohol reflux that volume fraction is 80%
It taking 1-2 times, each 2-3h, each solid-liquid ratio is 1:10g/mL, after standing 10-12h, the second for being again 80% with volume fraction
Alcohol reflux 1-2 times, each solid-liquid ratio are 1:8g/mL, are 55-65 DEG C in temperature after merging filtrate, and negative pressure is -0.06~-
It is concentrated under reduced pressure under 0.08Mpa, after recycling ethyl alcohol, spray drying obtains powder two;
Step D, powder one is mixed with powder two, obtains bamboo extractive.
8. the preparation method of the health food of functions of removing chloasma as claimed in claim 6, which is characterized in that the rose is extracted
The preparation method of object the following steps are included:
Step 1), by after fresh roseleaf impurity elimination, sterilization, grinding, the ferment for being rose weight 0.01-0.1% with weight
Mother and weight are that the water of 5-10 times of rose weight is uniformly mixed, and ferment 1-2 days at 25-30 DEG C, obtain tunning
Two;
Step 2), into tunning two be added weight be two weight 0.5-1% of tunning cellulase and weight
For the pectase of two weight 0.5-1% of tunning, 2-3h is digested in the case where temperature is 40-50 DEG C, pH value is 5-6, is filtered later,
Obtain filtrate two and filter residue two;
Step 3), by filter residue two immerse weight be 10-15 times of two weight of filter residue 20-50 times of liquid of bamboo vinegar or wood vinegar in, it is close
After envelope stands 10-12h, flows back 1 time, after filtering, obtain filtrate three and filter residue three;
Step 4), by filter residue three immerse weight be 7-10 times of three weight of filter residue 20-50 times of liquid of bamboo vinegar or wood vinegar in, it is close
After envelope stands 1-2h, assisting ultrasonic extracts 20-30min at being 30-40 DEG C in temperature, filters later, obtains filtrate four;
Step 5), by filtrate two, filtrate three and filtrate four mix after, temperature be 65-75 DEG C, negative pressure be -0.06~-
After being concentrated under reduced pressure under 0.08Mpa, spray drying obtains Flos Rosae Rugosae extract.
9. the preparation method of the health food of functions of removing chloasma as claimed in claim 5, which is characterized in that the step 2 is specific
Are as follows:
Medicinal extract is dispensed into each stainless steel disc, is put into 65-75 DEG C of baking oven, forced air drying to feel dry cream is hardened, it is touched
Take out, put to room temperature, left-hand thread stainless steel disc after not viscous, tapping is tried to get to the heart of a matter to dry cream fall off after remove, dry cream is collected, after drying
Medicinal extract crushes, and crosses 80 meshes, obtains extract powder.
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