CN110141542B - Allergy-relieving agent containing radix pseudostellariae and preparation method thereof - Google Patents
Allergy-relieving agent containing radix pseudostellariae and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a soothing and sensitizing agent containing radix pseudostellariae and a preparation method thereof, and the soothing and sensitizing agent mainly comprises 15-35% of radix pseudostellariae functional components, 10-20% of sodium hyaluronate, 4-8% of 1, 2-pentanediol and 40-71% of deionized water. The radix Pseudostellariae effective components are extracted from radix Pseudostellariae, fructus Hippophae, and Glycyrrhrizae radix. The soothing and sensitizing agent containing radix pseudostellariae provided by the invention has a good soothing and sensitizing effect, has a very obvious inhibiting effect on skin itch caused by histamine phosphate, has a higher safety factor compared with the existing acne removing products, and is simple in preparation process, lower in preparation cost and high in utilization rate of medicinal materials. The invention adopts unique Chinese medicine pseudostellaria root, sea buckthorn fruit and liquorice extract, contains natural detoxifying and anti-inflammatory factors, has good antibacterial and anti-inflammatory effects and high biological activity.
Description
Technical Field
The invention belongs to the technical field of cosmetic raw materials, and particularly relates to a soothing and sensitizing agent containing radix pseudostellariae and a preparation method thereof.
Background
With the continuous improvement of living standard of people, people pursue life not only on a level of being saturated. Under the condition of meeting basic living conditions, people pay more and more attention to the health problem of skin, and the attention to sensitive skin is also increased year by year.
The sensitive skin refers to the high reaction state of the skin under physiological or pathological conditions, and is clinically manifested as dry, red, blotchy, swollen eyes, desquamation or pimples on the surface of the skin. Sensitive skin is the result of multifactorial interactions. It can be classified into endogenous factors such as race, age, sex, heredity, endocrine factors, certain diseases, etc., and exogenous factors such as chemical substance stimulation, environmental factors, life style, psychological factors, etc. With the increasing environmental pollution and the increasing mental stress, the incidence of sensitive skin is gradually increased, and cosmetics for sensitive muscles are also put into more and more important positions.
With the improvement of the quality of life of people, the gap between the expectations for daily product function, safety and health and reality becomes larger and larger. The deterioration of the environment and the aggravation of competition further aggravate the distrust and uncertainty of people in mind for modern industrialized life. The allergy-relieving products in the current market mainly take bisabolol, dipotassium glycyrrhizinate and the like as main materials, but have the defects of poor solubility or slow allergy-relieving effect and the like. In order to make the product more prominent, some poor marketers add glucocorticoid ingredients such as glucocorticoid in the product, which takes effect quickly, but the long-term use of the product can cause the human body to have dependence on the hormone, and once the use of the cosmetic skin care product added with the hormone is stopped, the product can cause serious allergic symptoms and bring irreversible damage to the skin of the user.
The existing Chinese herbal medicine allergy-relieving agent generally comes from a hospital proved prescription, and the common prescription comprises the following components: cocklebur fruit, broom cypress fruit, chuanxiong rhizome, safflower, bittersweet herb, common cnidium fruit, lightyellow sophora root, densefruit pittany root-bark, snake slough and cicada slough, but most of the extraction methods adopt the traditional water extraction method and the like, the product has obvious effect, but the medicinal material loss is large, the product extraction rate is low, the impurities with non-allergy-relieving effect are more, and most of the colors are darker, so that the application in the preparation of cosmetics is difficult.
Chinese patent publication No. CN105287280A (application No. CN201510684956.2) discloses a plant soothing and sensitizing agent and a preparation method thereof, which adopts a method of combining an expanded bed and a fixed bed for extraction of effective components of plants, but the construction in the plant soothing and sensitizing agent is complex and has higher requirements on operation.
Chinese patent publication No. CN 102988242a (application No. 201210519947.4) discloses a method for preparing mung bean sprout extract and its use, wherein the mung bean sprout extract is used to prepare a soothing agent, but the mung bean sprout extract is extracted with ethanol, which has a certain sensitization risk.
The pseudostellaria root contains various chemical components including trace elements, amino acids, saccharides, nucleosides, phospholipids, cyclic peptides, fatty acids, oils and volatile components, and the pseudostellaria root polysaccharide can effectively inhibit the generation of MDA (multidrug resistance) through spectrophotometry determination, so that the allergy relieving effect is achieved.
Disclosure of Invention
In order to solve the problems, the invention provides a soothing agent containing radix pseudostellariae and a preparation method thereof. The radix pseudostellariae polysaccharide is applied to the allergy relieving agent, and is matched with the seabuckthorn fruits and the liquorice, so that the allergy relieving effect is more remarkable. The radix pseudostellariae is an excellent natural product, and has the advantages of obvious effect, high yield, wide distribution, low price, simple extraction process operation and low production cost.
The purpose of the invention is realized by the following technical scheme:
a allergy-relieving agent containing radix pseudostellariae is prepared from the following raw materials in percentage by weight: 15-35% of radix pseudostellariae functional components, 10-20% of sodium hyaluronate, 4-8% of 1, 2-pentanediol and 40-71% of deionized water, wherein the sum of the weight percentages of the components is 100%.
The radix pseudostellariae functional components are extracted from radix pseudostellariae, sea buckthorn fruit and liquorice.
The composite material is prepared from the following raw materials in percentage by weight: 23% of radix pseudostellariae functional components, 16% of sodium hyaluronate, 6% of 1, 2-pentanediol and 55% of deionized water.
Further, the effective components of the radix pseudostellariae are extracted from the raw materials of the radix pseudostellariae, sea buckthorn fruit and liquorice in a mass ratio of 3-4: 1-2: 1.
furthermore, the effective components of the radix pseudostellariae are extracted from the raw materials of the radix pseudostellariae, the sea buckthorn fruit and the liquorice in a mass ratio of 3.6: 1.4: 1.
the invention also provides a preparation method of the radix pseudostellariae functional components
(1) Crushing radix pseudostellariae in a traditional Chinese medicine crusher, sieving with a 60-mesh sieve to obtain radix pseudostellariae powder, and mixing the powder with deionized water according to a mass ratio of 1: reflux-extracting at 90 deg.C for 3 times at a ratio of 8-10 for 3h, 2h and 1h respectively; filtering under reduced pressure, mixing the filtrates, and concentrating to 0.5 g/mL;
(2) adding Sevag reagent (n-butyl alcohol: trichloromethane is 1: 4) into the filtrate obtained in the step (1), wherein the mass ratio of the filtrate: sevag reagent ═ 5: shaking for 5min to precipitate protein. Centrifuging at 3000r/min for 20min, removing lower protein emulsion layer, collecting supernatant, and repeating above operations until there is no milky floccule between the two phases; and concentrating the supernatant to 0.15g/mL, and freeze-drying to obtain the crude polysaccharide powder of the radix pseudostellariae.
(3) Dissolving the deproteinized radix pseudostellariae crude polysaccharide powder in distilled water, wherein the mass ratio of the crude polysaccharide powder to the protein powder is 1: 40, putting into a dialysis bag, dialyzing with distilled water at 4 ℃ for 48 hours, and replacing the distilled water every 6 hours; adding 3 times volume of anhydrous alcohol into the dialysate, filtering under reduced pressure, precipitating, and freeze drying to obtain refined radix Pseudostellariae polysaccharide powder.
(4) Mixing fructus Hippophae and Glycyrrhrizae radix, pulverizing in a traditional Chinese medicine pulverizer, sieving with a 60-mesh sieve, and mixing with deionized water at a material ratio of 1: 14, adopting an ultrasonic-assisted extraction method, setting the extraction temperature to be 70 ℃, the power to be 130W, extracting for 2 times, wherein the extraction time is 45min each time, and filtering under reduced pressure to obtain filtrate; mixing the filtrates, and rotary steaming at 60 deg.C to obtain fructus Hippophae and Glycyrrhrizae radix extractive solution.
(5) And (3) mixing 30-40 parts by weight of the refined radix pseudostellariae polysaccharide powder prepared in the step (3) with 10-20 parts by weight of the sea buckthorn fruit obtained in the step (4) and 10 parts by weight of the liquorice extract.
The invention also provides a preparation method of the allergy-relieving agent containing radix pseudostellariae, which comprises the following steps:
s1: mixing sodium hyaluronate and deionized water uniformly.
S2: 1, 2-pentanediol was added to the product obtained in step S1 and mixed uniformly.
S3: adding the radix pseudostellariae functional components into the product obtained in the step S2, uniformly mixing, stirring at the speed of 100-200 r/min for 5-15 min, and stirring at the temperature of less than or equal to 25 ℃.
S4: and (5) sterilizing the product obtained in the step (S3) at 80-100 ℃ for 20-40 minutes to obtain the desensitizer.
The invention has the beneficial effects that:
1. the radix pseudostellariae contains various chemical components including trace elements, amino acids, saccharides, nucleosides and the like, the pharmacological activity of the radix pseudostellariae mainly has the functions of enhancing immunity, resisting oxidation, stress, fatigue and the like, and the radix pseudostellariae polysaccharide can improve the activity of SOD and eliminate harmful substances generated in the metabolism process of organisms so as to achieve the functions of relieving allergy and relieving itching.
2. The sea buckthorn berries contain vitamin E, oleic acid, linoleic acid, palmitic acid, unsaturated fatty acid and the like, the roots, stems, leaves, flowers and fruits of the sea buckthorn berries, particularly the sea buckthorn berries, contain rich nutrient substances and bioactive substances, and the effective component superoxide dismutase (SOD) in the sea buckthorn berries has the function of removing free radicals in human bodies and has remarkable function of delaying the aging of the human bodies.
3. The main components of Glycyrrhrizae radix are triterpenes and flavonoids. It has effects of nourishing skin and moisturizing, repairing damaged skin and hair, inhibiting tyrosinase by Glycyrrhrizae radix, and whitening skin rapidly, and has oxygen preventing effect similar to that of superoxide dismutase.
4. The invention has reasonable formula, and the added pseudostellaria root functional components have good effects of relieving allergy and repairing skin barriers. Meanwhile, the product has certain anti-aging and whitening effects.
5. Compared with hormone-like soothing agents, the radix pseudostellariae functional components adopted by the invention have no symptoms after being stopped using and do not cause secondary damage to the skin. Is suitable for long-term use and has small dosage.
6. The method utilizes the characteristic that protein is denatured in organic solvents such as trichloroethane and the like to mix the radix pseudostellariae extracting solution and the Sevage reagent, and then the mixture is oscillated and centrifuged, and the denatured protein is positioned at the junction of the extracting solution and the Sevage reagent, so that polysaccharide is not precipitated, the protein precipitation reaction condition is mild, and the polysaccharide is not denatured.
7. The method refines the crude heterophylly falsestarwort root polysaccharide powder by using a dialysis method, has higher yield and polysaccharide content, is simple, consumes short time and has low cost, and meets the requirement of large-scale production.
8. The ingredients of the invention adopt unique Chinese herbal medicines including radix pseudostellariae, sea buckthorn fruit and liquorice extract, and the invention contains natural detoxification and anti-inflammatory factors, has small difficulty of the preparation process, simple extraction steps of radix pseudostellariae functional ingredients, high utilization rate of medicinal materials and low economic cost, but can also achieve good allergy relieving effect. Is suitable for adding into cosmetic production.
Detailed Description
In order to better understand the present invention, the following examples are further described, which are only used to explain the present invention and do not limit the present invention.
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
Example 1
A allergy-relieving agent containing radix pseudostellariae comprises the following components in percentage by weight: 23% of radix pseudostellariae functional components, 16% of sodium hyaluronate, 6% of 1, 2-pentanediol and 55% of deionized water. The effective components of the radix pseudostellariae are extracted from the raw materials of the radix pseudostellariae, sea buckthorn fruit and liquorice in a mass ratio of 3.6: 1.4: 1.
the preparation method of the radix pseudostellariae functional components comprises the following steps
(1) Crushing radix pseudostellariae in a traditional Chinese medicine crusher, sieving with a 60-mesh sieve, and mixing with deionized water according to a material ratio of 1: 10, reflux extracting for 3 times at 90 ℃, wherein the extracting time is 3h, 2h and 1h respectively; filtering under reduced pressure, mixing the filtrates, concentrating to 0.5g/mL,
(2) adding Sevag reagent (n-butyl alcohol: trichloromethane is 1: 4) into the filtrate obtained in the step (1), wherein the mass ratio of the filtrate: sevag reagent ═ 5: shaking for 5min to precipitate protein. Centrifuging at 3000r/min for 20min, removing lower protein emulsion layer, collecting supernatant, and repeating above operations until there is no milky floccule between two phases; and concentrating the supernatant to 0.15g/mL, and freeze-drying to obtain the crude polysaccharide powder of the radix pseudostellariae.
(3) Dissolving the deproteinized radix pseudostellariae crude polysaccharide powder in distilled water, wherein the mass ratio of the powder to the crude polysaccharide powder is 1: 40, putting into a dialysis bag, dialyzing with distilled water at 4 ℃ for 48 hours, and replacing the distilled water every 6 hours; adding 3 times volume of anhydrous alcohol into the dialysate, filtering under reduced pressure, precipitating, and freeze drying to obtain refined radix Pseudostellariae polysaccharide powder.
(4) Mixing fructus Hippophae and Glycyrrhrizae radix, pulverizing in a traditional Chinese medicine pulverizer, sieving with a 60-mesh sieve, and mixing with deionized water at a material ratio of 1: 14, adopting an ultrasonic-assisted extraction method, setting the extraction temperature to be 70 ℃, the power to be 130W, extracting for 2 times in 45min, and filtering under reduced pressure to obtain filtrate; mixing the filtrates, and rotary steaming at 60 deg.C to obtain fructus Hippophae and Glycyrrhrizae radix extractive solution.
(5) And (4) mixing the refined radix pseudostellariae polysaccharide powder with the seabuckthorn fruit and liquorice extracting solution obtained in the step (4) according to the mass ratio of the raw materials.
The invention also provides a preparation method of the allergy-relieving agent containing radix pseudostellariae, which comprises the following steps:
s1: mixing sodium hyaluronate and deionized water uniformly.
S2: 1, 2-pentanediol was added to the product obtained in step S1 and mixed uniformly.
S3: adding the radix pseudostellariae functional components into the product obtained in the step S2, uniformly mixing, stirring at the speed of 100-200 r/min for 5-15 min, and stirring at the temperature of less than or equal to 25 ℃.
S4: sterilizing the product obtained in the step S3 at the temperature of 80-100 ℃ for 20-40 minutes to obtain the product
Example 2
A allergy-relieving agent containing radix pseudostellariae comprises the following components in percentage by weight: 13% of radix pseudostellariae functional components, 20% of sodium hyaluronate, 4% of 1, 2-pentanediol and 63% of deionized water. The effective components of the radix pseudostellariae are extracted from the raw materials of the radix pseudostellariae, sea buckthorn fruit and liquorice in a mass ratio of 3: 2: 1.
the preparation method is the same as that of the above example 1, and the details are not repeated here.
Example 3:
a allergy-relieving agent containing radix pseudostellariae comprises the following components in percentage by weight: the radix pseudostellariae health-care beverage comprises 34% of radix pseudostellariae functional components, 15% of sodium hyaluronate, 7% of 1, 2-pentanediol and 43% of deionized water. The effective components of the radix pseudostellariae are extracted from raw materials of the radix pseudostellariae, sea buckthorn fruit and liquorice in a mass ratio of 4: 1: 1.
the preparation method is the same as that of the above example 1, and the details are not repeated here.
Test example 1 (evaluation of physical and chemical indexes of the invention)
The characteristics are as follows: the soothing and sensitizing agent is in a light brown transparent viscous liquid state, has fine and smooth texture, is uniform and consistent, and has fragrant smell.
Checking the pH value: taking the product, measuring with pH meter at 25 deg.C to obtain pH value of 5.8-6.5.
Heat resistance test: the product is sealed in a container, kept in a thermostat at 40 ℃ for 24h, and does not separate after being restored to room temperature.
Cold resistance test: the product is sealed in a container, kept in a refrigerator at-4 ℃ for 24h, and recovered to room temperature, and then asexual difference is obtained before the test.
And (3) centrifugal test: the product is placed in a centrifuge at 3000r/min, and centrifuged for 20min without layering.
Test example 2 (evaluation of safety)
Test subjects: example 1 the prepared phlegmatiser.
The test population: 40, between 15-30 years old, 20 women, 20 men.
The healthy subject has no allergic history of skin diseases and accords with the volunteer selection standard of the subject.
The spot pasting method comprises the following steps: and (3) selecting a qualified spot tester, putting about 0.020-0.025 g of the tested object into the spot tester in a closed spot test mode, and keeping the control hole blank. The sample and blank control were applied to the curved side of the forearm of the subject for 24h, the patch was removed and the interval was 30min, and the skin reaction was observed after the indentation disappeared. The skin reaction was observed once more 24h and 48h after patch test, respectively. The results were recorded according to the skin reaction grading Standard in "standards for hygiene of cosmetics".
The result of the human body skin patch test shows that 40 subjects can observe skin reaction in 24 hours through the allergy-free agent patch test, wherein 0 case of the skin reaction has adverse skin reaction, which indicates that the allergy-free agent used in the test is safe.
Test example 3 (hyaluronidase inhibition test)
Test subjects: the soothing agent and dipotassium glycyrrhizinate prepared in examples 1-3
The test method comprises the following steps: adding 0.1ml of 0.25mmol/L calcium chloride solution and 0.5ml of hyaluronidase solution into a test tube, carrying out heat preservation culture at 37 ℃ for 20min, adding 0.5ml of sample solution, carrying out heat preservation culture at 37 ℃ for 20min, adding 0.5ml of sodium hyaluronate solution, carrying out heat preservation at 37 ℃ for 30min, standing at normal temperature for 5min, adding 0.1ml of 0.4mol/L sodium hydroxide solution and 0.5ml of acetylacetone solution, carrying out boiling water bath reaction for 15min, immediately carrying out ice bath for 5min, adding 1.0ml of Ellisib reagent, diluting with 3.0ml of absolute ethyl alcohol, carrying out color development after standing for 20min, measuring the absorbance of a device, and substituting the following formula to calculate the inhibition rate.
Hyaluronidase inhibition (%) - (A-B) - (C-D) ]/(A-B) × 100%
A: absorbance of control solution (acetate buffer instead of sample solution)
B: blank control solution Absorbance (acetate buffer solution was used instead of sample and enzyme solution)
C: absorbance value of test sample solution
D: absorbance of sample blank solution (using acetic acid buffer instead of enzyme solution)
The results of the experiments are shown in the table below
| Test sample | Example 1 | Example 2 | Example 3 | Glycyrrhizic acid dipotassium salt |
| Inhibition ratio (%) | 94.3 | 92.8 | 93.2 | 95.4 |
The experiment of inhibiting hyaluronidase shows that compared with industry-recognized dipotassium glycyrrhizinate with a allergy relieving effect, the invention has a good allergy relieving effect, the effect is not obvious compared with that of the dipotassium glycyrrhizinate, but the dipotassium glycyrrhizinate is a hormone-like substance, a certain drug dependence type possibly exists, and the safety coefficient is higher when the invention is used.
Test example 4 (itching relieving test using Guinea pig skin itch model)
Test subjects: the soothing agents prepared in examples 1 to 3.
A subject: healthy guinea pig 36
The test method comprises the following steps: 36 guinea pigs were shaved on the dorsum of the right and rear feet 1 day before the test, and the shaved parts were evenly smeared with the test substances in the corresponding doses randomly and continuously for 3 days, and the model control group was smeared with distilled water. On the 3 rd day of the test, a proper amount of histamine phosphate is precisely weighed and prepared into a gradient concentration of 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% and 0.10% by using distilled water for later use before use. The shaved area of the dorsum of the right hind foot of the guinea pig was scratched with coarse sandpaper. The area is about 1 square centimeter, the medicine is applied for 1 time again, 0.05mL of 0.01% histamine phosphate is dropped on the affected part 10min after the last application, and the concentration of the histamine phosphate is increased by 0.01%, 0.02%, 0.03% and 0.04% … every 3min, each time is 0.05 mL. Until the guinea pig licks the right hind paw, the total amount of histamine phosphate dripped when the guinea pig licks the right hind paw was finally found to be the scratchiness threshold. The itch thresholds were calculated for each group and the differences between groups were compared.
The results of the experiments are shown in the table below
| Test sample | Test example 1 | Test example 2 | Test example 3 |
| Itch causing valve (mug) | 360.2±120.8 | 355.4±95.4 | 300.4±82.6 |
A guinea pig skin itch model itching relieving test shows that the invention has extremely obvious inhibitory action on skin itch reaction caused by histamine phosphate, wherein the test example 1 has the strongest inhibitory action on the skin itch of guinea pigs caused by histamine phosphate and has obvious itching relieving efficacy.
Test example 5 (skin irritation recovery Effect test)
Test subjects: the comfort agent prepared in examples 1-3.
The test population: 8, between 15 and 30 years old, 4 women and 4 men.
The healthy subject has no allergic history of skin diseases and accords with the volunteer selection standard of the subject.
The test method comprises the following steps: selecting the left and right arm internal test of the subject as the tested part, circularly marking a square area with the specification of 2cm x 2cm, cleaning the tested area with clear water, sitting still for 10min, measuring the blank value of the skin red value, and stimulating the tested area with a capsaicin water solution with the mass fraction of 50%. And (3) performing data acquisition after continuously stimulating for 15min, smearing a sample on a tested area, performing data acquisition after 15min, 30min and 45min respectively, and replacing the sample with deionized water in a blank group.
The results are shown in the following table
| Percent change in Red (%) | 0min | 15min | 30min | 45min |
| Example 1 | 23 | 13 | 6 | 3 |
| Example 2 | 25 | 16 | 13 | 7 |
| Example 3 | 26 | 17 | 12 | 5 |
| Blank group | 24 | 22 | 17 | 13 |
The skin irritation repairing effect test results show that the skin after being stimulated by the capsaicin aqueous solution has a certain repairing function in the embodiments 1-3, wherein the skin repairing effect in the embodiment 1 is the best, and the change rate of the red value is reduced from 23% to 3% after the skin is smeared for 45 min.
Test example 3 (antibacterial Effect test)
The tested strains: propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis
Test subjects: example 1-3 desensitizing Agents
The test method comprises the following steps: dissolving the slant bacteria cultured in advance in 3ml sterile water in an ultra-clean bench, pouring the bacteria liquid into a clean conical flask for later use, sucking 150 mul of the bacteria liquid and a disposable culture medium by a pipette gun, and uniformly coating the bacteria liquid by an applicator. Sealing the culture medium with sealing film, placing the culture medium in a constant temperature incubator at 37 deg.C for one day without turning over. Slightly placing the sterilized Oxford cup on a culture medium in a clean bench, accurately weighing 0.05g of sample, adding the sample into the Oxford cup, placing the Oxford cup into a constant-temperature incubator at 37 ℃ for two days without sealing and reversing the culture medium, and measuring the inhibition zone.
The results are shown in the following table
| Group of | Escherichia coli | Staphylococcus aureus | Salmonella |
| Example 1 | 18.5mm | 16.0mm | 17.0mm |
| Example 2 | 17.0mm | 14.0mm | 15.0mm |
| Example 3 | 16.5mm | 15.5mm | 17.0mm |
The results of antibacterial experiments show that examples 1-3 have good inhibition effects on the generation of propionibacterium acnes, staphylococcus aureus and surface staphylococcus, and the comprehensive effect of example 1 is the best. The invention has good antibacterial effect on common sensitized bacteria, thereby inhibiting the occurrence of allergy.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
1. A allergy relieving agent containing radix pseudostellariae is characterized in that: the material is prepared from the following raw materials in percentage by weight: 15-35% of radix pseudostellariae functional components; 10-20% of sodium hyaluronate; 4-8% of 1, 2-pentanediol; 40-71% of deionized water, wherein the sum of the weight percentages of the components is 100%; the radix pseudostellariae functional components are extracted from the following raw materials in parts by weight: 30-40 parts of radix pseudostellariae polysaccharide powder; 10-20 parts of sea buckthorn fruits; 10 parts of liquorice; the preparation method of the radix pseudostellariae functional components comprises the following steps:
(1) mixing fructus Hippophae and Glycyrrhrizae radix, pulverizing in a traditional Chinese medicine pulverizer, sieving with a 60-mesh sieve, and mixing with deionized water at a material ratio of 1: 14, adopting an ultrasonic-assisted extraction method, setting the extraction temperature to be 70 ℃, the power to be 130W, extracting for 2 times, wherein the extraction time is 45min each time, and filtering under reduced pressure to obtain filtrate; mixing the filtrates, and rotary steaming at 60 deg.C to obtain fructus Hippophae and Glycyrrhrizae radix extractive solution;
(2) mixing the refined radix pseudostellariae polysaccharide powder with the seabuckthorn fruit and liquorice extract obtained in the step (1).
2. The soothing agent containing radix pseudostellariae according to claim 1, wherein the preparation method of the radix pseudostellariae polysaccharide powder is as follows:
a1, pulverizing radix Pseudostellariae in a traditional Chinese medicine pulverizer, sieving with a 60-mesh sieve to obtain radix Pseudostellariae powder, mixing with deionized water, and extracting under reflux at 90 deg.C for 3 times, wherein the extraction time is 3h, 2h and 1h respectively; filtering under reduced pressure, mixing the filtrates, and concentrating to 0.5 g/mL;
a2, adding Sevag reagent into the filtrate obtained in the step A1, and shaking for 5min to separate out protein; centrifuging at 3000r/min for 20min, removing lower protein emulsion layer, collecting supernatant, and repeating above operations until there is no milky floccule between two phases; concentrating the supernatant to 0.15g/mL, and freeze drying to obtain radix Pseudostellariae crude polysaccharide powder;
a3, dissolving the deproteinized crude polysaccharide powder of radix Pseudostellariae in distilled water, putting into a dialysis bag, dialyzing with distilled water at 4 deg.C for 48h, and changing distilled water every 6 h; adding 3 times volume of anhydrous alcohol into the dialysate, filtering under reduced pressure, precipitating, and freeze drying to obtain refined radix Pseudostellariae polysaccharide powder.
3. The soothing agent containing radix pseudostellariae according to claim 2, which is characterized in that: the mass ratio of the radix pseudostellariae powder to the deionized water in the step A1 is 1: 8-10.
4. The soothing agent containing radix pseudostellariae according to claim 3, which is characterized by comprising the following components in percentage by weight: the mass ratio of the filtrate to the Sevag reagent is 5: 1.
5. the soothing agent containing radix pseudostellariae according to claim 3, wherein: the Sevag reagent is a mixed solution of n-butyl alcohol and trichloromethane with the mass ratio of 1: 4.
6. The soothing agent containing radix pseudostellariae according to claim 3, wherein: the mass ratio of the radix pseudostellariae crude polysaccharide powder in the step A3 to the distilled water in the step A3 is 1: 40.
7. a process for preparing a soothing agent containing radix pseudostellariae according to any one of claims 1-6, comprising the steps of:
s1: uniformly mixing sodium hyaluronate with deionized water;
s2: adding 1, 2-pentanediol into the product obtained in the step S1 and uniformly mixing;
s3: adding the radix pseudostellariae functional components into the product obtained in the step S2, and stirring and mixing uniformly;
s4: and (5) sterilizing the product obtained in the step S3 at the temperature of 80-100 ℃ for 20-40 minutes to obtain the soothing agent.
8. The preparation method of the soothing agent containing radix pseudostellariae according to claim 7, wherein the preparation method comprises the following steps: the stirring speed of the step S3 is 100-200 r/min, the stirring time is 5-15 min, and the stirring temperature is less than or equal to 25 ℃.
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