CN110141542A - A kind of Shu Min agent and preparation method thereof containing radix pseudostellariae - Google Patents
A kind of Shu Min agent and preparation method thereof containing radix pseudostellariae Download PDFInfo
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Abstract
The Shu Min agent and preparation method thereof containing radix pseudostellariae that the invention discloses a kind of, main component are radix pseudostellariae functional component 15~35%, Sodium Hyaluronate 10~20%, 1,2- pentanediol 4~8%, deionized water 40 ~ 71%.Radix pseudostellariae functional component extracts gained by radix pseudostellariae, fructus hippophae, Radix Glycyrrhizae.Shu Min agent provided by the invention containing radix pseudostellariae has good Shu Min effect, has extremely significant inhibiting effect to the reaction of pruitus caused by histamine phosphate, safety coefficient is higher compared with existing product for resolving poxes, and manufacture craft is simple, and cost of manufacture is lower, high to medicinal material utilization rate.Present component uses unique Chinese medicine radix pseudostellariae, fructus hippophae, and licorice has good antimicrobial antiphlogistic to act on containing the natural detoxicating, relieving inflammation factor, and bioactivity is high.
Description
Technical field
The invention belongs to the technical fields of cosmetic material, and in particular to a kind of Shu Min agent and its preparation side containing radix pseudostellariae
Method.
Background technique
With the continuous improvement of people's living standards, people not only rest in the level of having enough to eat and wear the pursuit of life.?
In the case of meeting basic living condition, people increasingly pay attention to the health problem of skin, to the attention rate of sensitive skin also by
Year rises.
Sensitive refers in particular to the high reactiveness that skin occurs under physiology or pathological conditions, and clinical manifestation is skin
Dry tack free, it is rubescent, play spot, eye swollen, decortication or raw dark sore etc..Sensitive is multifactor coefficient result.It can
It is divided into endogenous factor such as race, age, or sex, heredity, endocrine factors, certain diseases etc. and extrinsic factor as changed
Learn material incentive, environmental factor, life style, psychological factor etc..As the increasingly exacerbation of environmental pollution and stress increase,
The incidence of sensitive is gradually increased, and is also put in more and more important position for the cosmetics of sensitive flesh.
With the improvement of people's life quality, to the difference between the expectation and reality of daily chemical products function, safety and health
Away from becoming increasing.The deterioration of environment, the aggravation of competition have more aggravated distrust of the people in the heart to modern industrialization life
And uncertainty.Shu Min product currently on the market is mainly with bisabolol, based on dipotassium glycyrrhizinate etc., but has dissolubility not
The disadvantages of good or Shu Min effect is slow.More there are some wicked businesses more to protrude for product effect, adds sugared skin in the product
The hormones constituents such as matter, it is quick, but being used for a long time can make human body generate dependence to it, once stop using addition to swash
Serious allergic symptom, will occur in the cosmetic skin care product of element, carry out irreversible injury to the skin tape of user.
Current Chinese herbal medicine Shu Min agent is typically all to be derived from hospital's proved recipe, and common prescription has: the achene of Siberian cocklebur, the fruit of summer cypress, river
Bow, safflower, bittersweet, frutus cnidii, kuh-seng, cortex dictamni, snake slough, cicada slough, but extracting method largely uses traditional water extraction etc.,
Its product has positive effect, but medicinal material loss is big, and product recovery rate is small, and the impurity of Fei Shumin effect is more, and color is mostly relatively deep,
It is dfficult to apply in the production of cosmetics.
The Chinese patent literature of publication number CN105287280A (application number CN201510684956.2) discloses a plant
Object Shu Min agent and preparation method thereof, the method for using expansion bed to combine extraction with fixed bed carry out effective component to plant
It extracts, but building is complicated in it, to the more demanding of operation.
The Chinese patent literature of publication number CN 102988242A (application number 201210519947.4) discloses Mung Bean
The preparation method and its usage of extracting solution, wherein making Shu Min agent, but its green bean sprout extract using green bean sprout extract
It is extracted using ethyl alcohol, there is certain sensitization risk.
" radix pseudostellariae chemical component and its Advance on Pharmacological Activities " proposition that Chu Shuhao, Wang little Cai, Feng Liang are delivered, too
Containing there are many chemical components, including microelement, amino acid, carbohydrate, ucleosides, phospholipid, cyclic peptide, fatty acid in son ginseng
Class, grease type, volatile component etc. wherein, and are measured by spectrophotometry and are found, Pseudostellaria Polysaccharide can effectively inhibit
The generation of MDA, to achieve the effect that Shu Min.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of Shu Min agent and preparation method thereof containing radix pseudostellariae.Its ingredient is green
Color safety, Shu Min significant effect, extraction process for preparation is simple and safe, and medicinal material utilization rate is high, and impurity in products is few, is suitable for cosmetics
Is applied in Shu Min agent using Pseudostellaria Polysaccharide in production, then cooperates fructus hippophae and Radix Glycyrrhizae, keeps its Shu Min effect more significant.
Radix pseudostellariae itself is used as a kind of excellent natural products, and obvious effect, yield is high, and distribution is wide, and price is more cheap, extracts work
Skill operation is relatively simple, and production cost is low.
The purpose of the present invention is what is be achieved by the following technical programs:
A kind of Shu Min agent containing radix pseudostellariae, is made of the raw material of following weight percent: radix pseudostellariae functional component 15~
35%, Sodium Hyaluronate 10~20%, 1,2- pentanediol 4~8%, deionized water 40~71%, weight percentage of each component it
Be 100%.
Above-mentioned radix pseudostellariae functional component is with radix pseudostellariae, fructus hippophae, and Radix Glycyrrhizae is that raw material extracts gained.
It is specifically made of following weight percent raw material: radix pseudostellariae functional component 23%, Sodium Hyaluronate 16%, 1,2- penta
Glycol 6%, deionized water 55%.
Further, the radix pseudostellariae functional component extracts raw material radix pseudostellariae, fructus hippophae, and the mass ratio of Radix Glycyrrhizae is 3~4:1
~2:1.
Further, the radix pseudostellariae functional component extracts raw material radix pseudostellariae, and the mass ratio of fructus hippophae, Radix Glycyrrhizae is 3.6:
1.4:1.
The present invention also provides the preparation methods of radix pseudostellariae functional component
(1) radix pseudostellariae addition medicinal herb grinder is crushed, crosses 60 meshes and obtain Heterophylly Falsesatarwort Root, with deionized water material
The ratio of mass ratio 1:8-10,90 DEG C refluxing extraction 3 times, extraction time is respectively 3h, 2h and 1h；After being filtered under diminished pressure, by filtrate
Mixing, is concentrated into 0.5g/mL；
(2) (n-butanol: chloroform=1: 4), mass ratio is filter to the filtrate addition Sevag reagent obtained step (1)
Liquid: Sevag reagent=5:1 shakes 5min, protein is precipitated.It is centrifuged 20min with 3000r/min, removes sub-cloud protein emulsifying
Layer, takes supernatant to repeat above operation, until two alternate agalasisa White Flocculus；Supernatant is concentrated into 0.15g/mL, is freezed
It is dry, obtain radix pseudostellariae Thick many candies powder.
(3) the radix pseudostellariae Thick many candies powder after removing protein is dissolved in distilled water, mass ratio 1:40, is put into bag filter, 4
At DEG C after distilled water dialysis 48h, every 6h changes primary distilled water；The dehydrated alcohol of 3 times of volumes of dialyzate is added, is filtered under diminished pressure, sinks
It forms sediment and is freeze-dried, Pseudostellaria Polysaccharide powder must be refined.
(4) fructus hippophae and Radix Glycyrrhizae are mixed into medicinal herb grinder to crush, cross 60 meshes, with deionized water material ratio 1:
14 ratio sets 70 DEG C of Extracting temperature, power 130W using ultrasonic wave assisted extraction method, extracts 2 times, each extraction time
It for 45min, is filtered under diminished pressure, obtains filtrate；Filtrate is mixed and is rotated, temperature 60 C is rotated, obtains fructus hippophae, licorice extract.
It (5) will be 10~20 parts obtained by the purification Pseudostellaria Polysaccharide powder of 30~40 parts by weight steps (3) preparation and step (4)
Fructus hippophae, the mixing of 10 parts by weight licorice extracts.
The present invention also provides a kind of the Shu Min agent preparation method containing radix pseudostellariae, step are as follows:
S1: Sodium Hyaluronate, deionized water are uniformly mixed.
S2: 1,2- pentanediol is added in step S1 gains and uniformly mixes.
S3: being added radix pseudostellariae functional component in step S2 gains and uniformly mix, and mixing speed is 100~200r/
Min, mixing time are 5~15min, whipping temp≤25 DEG C.
S4: step S3 gains are sterilized 20~40 minutes in 80~100 DEG C to get Shu Min agent.
Beneficial effects of the present invention:
1, radix pseudostellariae is containing there are many chemical component, including microelement, amino acid, carbohydrate, ucleosides etc., the medicines of radix pseudostellariae
Reason activity, which mainly has, increases immune, anti-oxidant, resisting stress, fatigue-resisting function, and Pseudostellaria Polysaccharide disappears SOD vigor can be improved
Except the harmful substance that organism generates in metabolic processes, to have the function that Shu Min is antipruritic.
2, containing vitamin E, oleic acid, linoleic acid, palmitinic acid, unsaturated fatty acid etc. in fructus hippophae, the root of fructus hippophae,
Nutriment and bioactive substance rich in stem, leaf, flower, fruit, particularly fructus hippophae, effective component is super in fructus hippophae
Superoxide dismutase (SOD) has the function of the removal intracorporal free radical of people, and the aging of delaying human body has obvious action.
3, Radix Glycyrrhizae main component is triterpenes and flavone compound.The wet effect of its benefit nutritious to skin, and to damage
Skin, the hair of wound have maintenance effect, and Radix Glycyrrhizae has inhibiting effect to tyrosinase, the efficiency with quick brilliant white, with peroxidating
Mutase has similar oxygen ability.
4, the formula of the invention is reasonable, and the radix pseudostellariae functional component of addition has good Shu Min and repairs skin barrier effect
Fruit.Also there is certain anti-aging, white-skinned face function simultaneously.
5, for the present invention using radix pseudostellariae functional component compared to steroids Shu Min agent, illness phenomenon after not deactivating will not
Secondary damage is carried out to skin.It is suitble to be used for a long time, and dosage is small.
6, the present invention using protein be denaturalized in the organic solvents such as trichloroethanes the characteristics of, by radix pseudostellariae extracting solution with
The mixing of Sevage reagent, vibrates, and centrifugation, the protein after denaturation makes polysaccharide not between extracting solution and Sevage reagent intersection
It precipitates and keeps protein precipitation reaction condition mild, the denaturation of polysaccharide will not be caused.
7, the present invention refines thick Pseudostellaria Polysaccharide powder using dialysis, and yield and polyoses content are higher, method
Simply, time-consuming short, at low cost, meet the needs of mass production.
8, present component uses unique Chinese herbal medicine radix pseudostellariae, fructus hippophae, and licorice contains natural detoxicating, relieving inflammation
The factor, manufacture craft difficulty is small, and the extraction step of radix pseudostellariae functional component is simple, and medicinal material utilization rate is high, and economic cost is few, but same
Sample can achieve good Shu Min effect.It is suitable for addition in Cosmetic Manufacture.
Specific embodiment
In order to better understand the present invention, below by embodiment to the present invention into further explanation, embodiment is served only for
It explains the present invention, any restriction can't be constituted to the present invention.
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Embodiment 1
A kind of Shu Min agent containing radix pseudostellariae, ingredient and its weight percent are as follows: radix pseudostellariae functional component 23%, hyaluronic acid
Sodium 16%, 1,2- pentanediol 6%, deionized water 55%.The radix pseudostellariae functional component extracts raw material radix pseudostellariae, and fructus hippophae is sweet
The mass ratio of grass is specially 3.6:1.4:1.
Wherein the preparation method of the radix pseudostellariae functional component is
(1) radix pseudostellariae is crushed into medicinal herb grinder, crosses 60 meshes, with the ratio of deionized water material ratio 1:10,
90 DEG C refluxing extraction 3 times, extraction time is respectively 3h, 2h and 1h；After being filtered under diminished pressure, filtrate is mixed, is concentrated into 0.5g/mL,
(2) (n-butanol: chloroform=1: 4), mass ratio is filter to the filtrate addition Sevag reagent obtained step (1)
Liquid: Sevag reagent=5:1 shakes 5min, protein is precipitated.It is centrifuged 20min with 3000r/min, removes sub-cloud protein emulsifying
Layer, takes supernatant to repeat above operation, until two alternate agalasisa White Flocculus；Supernatant is concentrated into 0.15g/mL, is freezed
It is dry, obtain radix pseudostellariae Thick many candies powder.
(3) the radix pseudostellariae Thick many candies powder after removing protein is dissolved in distilled water, mass ratio 1:40, is put into bag filter, 4
At DEG C after distilled water dialysis 48h, every 6h changes primary distilled water；The dehydrated alcohol of 3 times of volumes of dialyzate is added, is filtered under diminished pressure, sinks
It forms sediment and is freeze-dried, Pseudostellaria Polysaccharide powder must be refined.
(4) fructus hippophae and Radix Glycyrrhizae are mixed into medicinal herb grinder to crush, cross 60 meshes, with deionized water material ratio 1:
14 ratio is set 70 DEG C of Extracting temperature, power 130W, 45min extraction 2 times, was depressurized using ultrasonic wave assisted extraction method
Filter, obtains filtrate；Filtrate is mixed and is rotated, temperature 60 C is rotated, obtains fructus hippophae, licorice extract.
(5) fructus hippophae obtained by Pseudostellaria Polysaccharide powder and step (4), licorice extract will be refined by material quality than mixing
It closes.
The present invention also provides a kind of the Shu Min agent preparation method containing radix pseudostellariae, step are as follows:
S1: Sodium Hyaluronate, deionized water are uniformly mixed.
S2: 1,2- pentanediol is added in step S1 gains and uniformly mixes.
S3: being added radix pseudostellariae functional component in step S2 gains and uniformly mix, and mixing speed is 100~200r/
Min, mixing time are 5~15min, whipping temp≤25 DEG C.
S4: by step S3 gains in 80~100 DEG C sterilize 20~40 minutes to get
Embodiment 2
A kind of Shu Min agent containing radix pseudostellariae, ingredient and its weight percent are as follows: radix pseudostellariae functional component 13%, hyaluronic acid
Sodium 20%, 1,2- pentanediol 4%, deionized water 63%.The radix pseudostellariae functional component extracts raw material radix pseudostellariae, and fructus hippophae is sweet
The mass ratio of grass is specially 3:2:1.
Preparation method, with above-described embodiment 1, details are not described herein again.
Embodiment 3:
A kind of Shu Min agent containing radix pseudostellariae, ingredient and its weight percent are as follows: radix pseudostellariae functional component 34%, hyaluronic acid
Sodium 15%, 1,2- pentanediol 7%, deionized water 43%.The radix pseudostellariae functional component extracts raw material radix pseudostellariae, and fructus hippophae is sweet
The mass ratio of grass is specially 4:1:1.
Preparation method, with above-described embodiment 1, details are not described herein again.
Test example 1 (physical and chemical index assessment of the present invention)
Character: Ben Shumin agent is in filbert hyalomucoid shape, and quality is fine and smooth, uniformity, fragrant.
PH value inspection: taking this product, is measured in 25 DEG C with pH meter, measures pH value between weak acid 5.8-6.5.
Heat resistant test: by this product loaded on sealing in container, keeping for 24 hours in 40 DEG C of insulating boxs, without separation after recovery room temperature
Phenomenon.
Low temperature resistant test: by this product loaded on sealing in container, keeping for 24 hours in -4 DEG C of refrigerators, after recovery room temperature and before test
Without Traits change.
Centrifugal test: this product is placed in a centrifuge 3000r/min, is centrifuged 20min, no lamination.
Test example 2 (safety evaluation)
Subjects: Shu Min agent prepared by embodiment 1.
Tested crowd: 40 people, between 15~30 years old, 20 people of female, male 20 people.
Health status subject meets subject and volunteers inclusion criteria without skin disease allergies.
Patch method: selecting qualified spot tester, with closed patch experiment method, by tested material about 0.020~
0.025g is put into patch device, and control wells are blank.Sample and blank control are affixed on to the forearm song side of subject, continued
For 24 hours, it is spaced 30min after removing patch device, observes dermoreaction after impression disappearance.It is seen again respectively with 48h for 24 hours after patch test
Examine primary, observation dermoreaction.Its result is recorded according to dermoreaction grade scale in " cosmetics health specification ".
Human skin patch test result shows 40 subjects Shu Min agent patch test through the invention,
Hour observes dermoreaction for 24 hours, wherein 0 skin adverse reaction occurs, illustrates to test Shu Min agent safety used.
Test example 3 (inhibits hyaluronidase test)
Subjects: the configured Shu Min agent of Examples 1 to 3 and dipotassium glycyrrhizinate
Test method: being added 0.1ml0.25mmol/L calcium chloride solution and 0.5ml hyaluronidase solution in test tube,
37 DEG C of heat insulating culture 20min, addition 0.5ml sample liquid, 37 DEG C of heat insulating culture 20min, addition 0.5ml Sodium Hyaluronate liquid, 37
DEG C heat preservation 30min, then room temperature stands 5min, and 0.4mol/L sodium hydroxide solution 0.1ml and acetylacetone,2,4-pentanedione solution is added
0.5ml, boiling water bath react ice bath 5min immediately after 15min, add 1.0ml Ehrlich's reagent and with 3.0ml dehydrated alcohol
It is diluted, develops the color after placing 20min, analyzer light absorption value substitutes into following formula and calculates inhibiting rate.
Hyaluronic acid enzyme inhibition rate (%)=[(A-B)-(C-D)]/(A-B) * 100%
A: contrast solution absorbance (replaces sample solution with acetate buffer solution)
B: placebo solution absorbance (replaces sample and enzyme solution with acetate buffer solution)
C: test sample solution absorbance value
D: sample blank solution absorbance value (replaces enzyme solution with acetate buffer solution)
Experimental result such as following table
| Test sample | Embodiment 1 | Embodiment 2 | Embodiment 3 | Dipotassium glycyrrhizinate |
| Inhibiting rate (%) | 94.3 | 92.8 | 93.2 | 95.4 |
For inhibiting hyaluronidase test to show the dipotassium glycyrrhizinate with Shu Min effect generally acknowledged compared to industry, this
Invention has good Shu Min effect, though effect is not significant compared with dipotassium glycyrrhizinate, dipotassium glycyrrhizinate is a kind of parahormone
Substance, it is understood that there may be certain pharmacological dependence type is higher using safety coefficient of the present invention.
Test example 4 (the antipruritic test of guinea pig skin itch model)
Subjects: Shu Min agent prepared by Examples 1 to 3.
Study subject: healthy guinea pig 36
Test method: taking cavy 36, the instep shaving after testing preceding 1 day right side, and random grouping is punished in shaving for three days on end
Corresponding dosage is not smeared not uniformly by test product, and model control group gives distilled water smearing.It tests the 3rd day, precision weighs histamine phosphate
In right amount, be made into 0.01% before use with distilled water, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%,
0.08%, 0.09%, 0.10% gradient concentration is spare.It will be abraded at instep shaving behind the cavy right side with coarse sandpaper.About 1 square of area
Centimetre, part coating 1 time again, after last coating 10min abrasion drip 0.01% histamine phosphate 0.05mL, later every
3min is 0.05mL according to 0.01%, 0.02%, 0.03%, 0.04% ... progressive concentration every time.It is later licked until there is cavy
Right metapedes drips the histamine phosphate total amount taken as itch-threshold when cavy later licks right metapedes finally to occur.Each group cause is calculated to itch
Otherness between threshold and comparative group.
Experimental result such as following table
| Test sample | Test example 1 | Test example 2 | Test example 3 |
| Valve (μ g) is itched in cause | 360.2±120.8 | 355.4±95.4 | 300.4±82.6 |
The antipruritic test test display present invention of guinea pig skin itch model has the reaction of pruitus caused by histamine phosphate
Extremely significant inhibiting effect, pilot scale example 1 are most strong to guinea pig skin itch inhibiting effect caused by histamine phosphate, have significant
Itching-relieving efficacies.
Test example 5 (test of skin irritatin repairing effect)
Subjects: Shu Min agent prepared by Examples 1 to 3.
Tested crowd: 8 people, between 15~30 years old, 4 people of female, male 4 people.
Health status subject meets subject and volunteers inclusion criteria without skin disease allergies.
Test method: selection subject is left, surveys in right arm and is used as recipient site, is the squared region of 2cm*2cm with specification
Domain carry out cycle labeling, clean tested area with clear water, sit quietly 10min, carry out skin red value blank value measure, after use mass fraction
Tested region is stimulated for 50% capsaicine aqueous solution.Data acquisition is carried out after persistently stimulating 15min, in tested region
Sample is smeared, 15min, 30min carry out data acquisition after 45min respectively, and blank group replaces sample with deionized water.
As a result as shown in the table
| Red value change rate (%) | 0min | 15min | 30min | 45min |
| Embodiment 1 | 23 | 13 | 6 | 3 |
| Embodiment 2 | 25 | 16 | 13 | 7 |
| Embodiment 3 | 26 | 17 | 12 | 5 |
| Blank group | 24 | 22 | 17 | 13 |
Skin irritatin repairing effect test result shows that Examples 1 to 3 has the post-stimulatory skin of capsaicine aqueous solution
A degree of repair function, wherein embodiment 1 is best to skin repair effect, and red value change rate is by 23% after smearing 45min
Drop to 3%.
Test example 3 (antibacterial effect test)
Tested strain: propionibacterium acnes, staphylococcus aureus, staphylococcus epidermis
Subjects: Shu Min agent prepared by Examples 1 to 3
Test method: the inclined-plane bacterium cultivated in advance is dissolved in super-clean bench with 3ml sterile water, bacterium solution is poured on completely
Conical flask in it is spare, drawn on 150 μ l bacterium solutions and batch cultur base with liquid-transfering gun, smoothened bacterium solution with spreader.With envelope
Membrana oralis seals culture medium, and culture medium is not buckled to, and is put into 37 DEG C of constant incubator and cultivates one day.In superclean bench,
Sterilized Oxford cup is put down gently on culture medium, 0.05g sample is accurately weighed and is added in Oxford cup, culture medium open-ended, no
Back-off, is put into 37 DEG C of constant incubators and cultivates two days, measures inhibition zone.
As a result as shown in the table
| Group | Escherichia coli | Staphylococcus aureus | Salmonella |
| Embodiment 1 | 18.5mm | 16.0mm | 17.0mm |
| Embodiment 2 | 17.0mm | 14.0mm | 15.0mm |
| Embodiment 3 | 16.5mm | 15.5mm | 17.0mm |
Antibacterial experiment the results show that Examples 1 to 3 for propionibacterium acnes, staphylococcus aureus, surface grape ball
Bacterium, which generates, good inhibiting effect, and the resultant effect of embodiment 1 is best.It is good to illustrate that the present invention has common sensitized bacteria
Good antibacterial effect, to inhibit the appearance of allergic phenomena.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (10)
1. a kind of Shu Min agent containing radix pseudostellariae, it is characterised in that: be made of the raw material of following weight percent: radix pseudostellariae effect at
Divide 15~35%；Sodium Hyaluronate 10~20%；1,2- pentanediol 4 ~ 8%；Deionized water 40 ~ 71%, weight percentage of each component it
Be 100%.
2. a kind of Shu Min agent containing radix pseudostellariae according to claim 1, which is characterized in that the radix pseudostellariae functional component by
Following weight parts raw material, which extracts, to be formed: 30 ~ 40 parts of Pseudostellaria Polysaccharide powder；10 ~ 20 parts of fructus hippophae；10 parts of Radix Glycyrrhizae.
3. a kind of Shu Min agent containing radix pseudostellariae according to claim 2, which is characterized in that the radix pseudostellariae functional component
The preparation method is as follows:
(1) fructus hippophae and Radix Glycyrrhizae are mixed into medicinal herb grinder to crush, 60 meshes are crossed, with deionized water material ratio 1:14's
Ratio sets 70 DEG C of Extracting temperature, power 130W using ultrasonic wave assisted extraction method, extracts 2 times, and each extraction time is
45min is filtered under diminished pressure, and obtains filtrate；Filtrate is mixed and is rotated, temperature 60 C is rotated, obtains fructus hippophae and licorice extract；
(2) purification Pseudostellaria Polysaccharide powder is mixed with fructus hippophae obtained by step (1) and licorice extract.
4. a kind of Shu Min agent containing radix pseudostellariae according to claim 2, which is characterized in that the Pseudostellaria Polysaccharide powder
The preparation method is as follows:
A1, radix pseudostellariae addition medicinal herb grinder is crushed, crosses 60 meshes and obtain Heterophylly Falsesatarwort Root, is mixed with deionized water, 90
DEG C refluxing extraction 3 times, extraction time is respectively 3h, 2h and 1h；After being filtered under diminished pressure, filtrate is mixed, is concentrated into 0.5g/mL；
Sevag reagent is added in A2, the filtrate for obtaining step A1, shakes 5min, protein is precipitated；With 3000 r/min centrifugation
20 min remove sub-cloud protein emulsifying layer, supernatant are taken to repeat above operation, until two alternate agalasisa White Flocculus；It will be upper
Clear liquid is concentrated into 0.15g/mL, and freeze-drying obtains radix pseudostellariae Thick many candies powder；
A3, the radix pseudostellariae Thick many candies powder after removing protein is dissolved in distilled water, is put into bag filter, distilled water dialysis 48h at 4 DEG C
Afterwards, every 6h changes primary distilled water；The dehydrated alcohol of 3 times of volumes of dialyzate is added, is filtered under diminished pressure, pellet frozen is dry, must refine
Pseudostellaria Polysaccharide powder.
5. a kind of Shu Min agent containing radix pseudostellariae according to claim 4, it is characterised in that: radix pseudostellariae powder described in step A1
The mass ratio of end and deionized water is 1:8-10.
6. a kind of Shu Min agent containing radix pseudostellariae according to claim 4, it is characterised in that: the filtrate and Sevag reagent
Mass ratio be 5:1.
7. a kind of Shu Min agent containing radix pseudostellariae according to claim 4, it is characterised in that: the Sevag reagent is positive fourth
The mixed solution that alcohol and chloroform mass ratio are 1: 4.
8. a kind of Shu Min agent containing radix pseudostellariae according to claim 4, it is characterised in that: the radix pseudostellariae in the step A3
The mass ratio of Thick many candies powder and the distilled water in step A3 is 1:40.
9. a kind of preparation method of the Shu Min agent containing radix pseudostellariae, which comprises the following steps:
S1: Sodium Hyaluronate and deionized water are uniformly mixed；
S2: 1,2- pentanediol is added in step S1 gains and uniformly mixes；
S3: radix pseudostellariae functional component is added in step S2 gains, is uniformly mixed；
S4: step S3 gains are sterilized 20 ~ 40 minutes in 80 ~ 100 DEG C to get Shu Min agent.
10. a kind of preparation method of Shu Min agent containing radix pseudostellariae according to claim 9, it is characterised in that: the step
The mixing speed of S3 is 100 ~ 200r/min, and mixing time is 5 ~ 15min, whipping temp≤25 DEG C.
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| CN111647091A (en) * | 2020-05-27 | 2020-09-11 | 广东药科大学 | Radix pseudostellariae active hexa-carbon aldehyde oligosaccharide and preparation method and application thereof |
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