CN109971684A - A kind of lactobacillus-fermented preparation and its activation method - Google Patents
A kind of lactobacillus-fermented preparation and its activation method Download PDFInfo
- Publication number
- CN109971684A CN109971684A CN201910282783.XA CN201910282783A CN109971684A CN 109971684 A CN109971684 A CN 109971684A CN 201910282783 A CN201910282783 A CN 201910282783A CN 109971684 A CN109971684 A CN 109971684A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- fermented
- fermented preparation
- preparation
- lactic acid
- Prior art date
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- Pending
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- 238000002360 preparation method Methods 0.000 title claims abstract description 89
- 230000004913 activation Effects 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 84
- 241000894006 Bacteria Species 0.000 claims abstract description 78
- 239000004310 lactic acid Substances 0.000 claims abstract description 42
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 16
- 239000003223 protective agent Substances 0.000 claims abstract description 12
- 241000186660 Lactobacillus Species 0.000 claims abstract description 10
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 10
- 238000005507 spraying Methods 0.000 claims abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 26
- 230000003213 activating effect Effects 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 13
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 10
- 230000008859 change Effects 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 7
- 108010080698 Peptones Proteins 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 235000019319 peptone Nutrition 0.000 claims description 7
- 229940074404 sodium succinate Drugs 0.000 claims description 7
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 235000019629 palatability Nutrition 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 239000003085 diluting agent Substances 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 5
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 claims description 4
- 229920002261 Corn starch Polymers 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 229940099112 cornstarch Drugs 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 4
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- STZCRXQWRGQSJD-GEEYTBSJSA-M methyl orange Chemical compound [Na+].C1=CC(N(C)C)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-GEEYTBSJSA-M 0.000 claims description 4
- 229940012189 methyl orange Drugs 0.000 claims description 4
- 239000011814 protection agent Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- 229920001353 Dextrin Polymers 0.000 claims description 3
- 239000004375 Dextrin Substances 0.000 claims description 3
- 241000194031 Enterococcus faecium Species 0.000 claims description 3
- 108010058643 Fungal Proteins Proteins 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 3
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 3
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 3
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- 239000005862 Whey Substances 0.000 claims description 3
- 102000007544 Whey Proteins Human genes 0.000 claims description 3
- 108010046377 Whey Proteins Proteins 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 235000019425 dextrin Nutrition 0.000 claims description 3
- -1 galactolipin Substances 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 3
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 3
- 239000008101 lactose Substances 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- 241000186679 Lactobacillus buchneri Species 0.000 claims description 2
- 244000199866 Lactobacillus casei Species 0.000 claims description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 2
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims description 2
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims description 2
- 241000186869 Lactobacillus salivarius Species 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 2
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 2
- 239000005018 casein Substances 0.000 claims description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000021240 caseins Nutrition 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 241000282941 Rangifer tarandus Species 0.000 claims 1
- 235000020167 acidified milk Nutrition 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- 239000008267 milk Substances 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 239000000052 vinegar Substances 0.000 claims 1
- 235000021419 vinegar Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 20
- 244000005700 microbiome Species 0.000 abstract description 9
- 238000011081 inoculation Methods 0.000 abstract description 4
- 230000002503 metabolic effect Effects 0.000 abstract description 4
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 241000235342 Saccharomycetes Species 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 description 10
- 230000000813 microbial effect Effects 0.000 description 8
- 239000000654 additive Substances 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 239000012669 liquid formulation Substances 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- 230000007423 decrease Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 235000020183 skimmed milk Nutrition 0.000 description 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- 240000006024 Lactobacillus plantarum Species 0.000 description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 3
- 229940043376 ammonium acetate Drugs 0.000 description 3
- 235000019257 ammonium acetate Nutrition 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 description 3
- 241000186840 Lactobacillus fermentum Species 0.000 description 2
- 241000283011 Rangifer Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000012766 Growth delay Diseases 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229960000448 lactic acid Drugs 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000009304 pastoral farming Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- 229960001790 sodium citrate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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- A23V2400/143—Fermentum
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/147—Helveticus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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- A23V2400/157—Lactis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/181—Salivarius
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
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Abstract
The present invention provides a kind of lactobacillus-fermented preparation and its activation method, the lactobacillus-fermented preparation is made of lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protective agent.A kind of lactobacillus-fermented preparation of the present invention is low in dry powdery, transportation cost, and low water activity can inhibit the metabolic activity of microorganism, is conducive to save the effective bacterium number of lactic acid bacteria；Fermentation preparation contains lactobacillus inoculation and Nutrious fermented object, activation act is easy, without equipment and energy consumption cost, lactic bacteria activity and bacterium number product control are stablized, user's acceptable degree is high, a kind of high-activity lactic acid bacteria bacterium solution of lactobacillus-fermented preparation activation method preparation is primarily useful for inoculation ensiling, biological fermentation feed or liquid state fermentation feeding system according to the present invention simultaneously, dominant strain can quickly be formed, reduce rapidly material pH value, a large amount of lactic acid are generated, the miscellaneous bacterias such as mould, saccharomycete and the growth of other harmful bacterias are inhibited；It can also be used to directly puddle feed feeding, spraying feeding etc..
Description
Technical field
The present invention relates to technical field of microbial fermentation, and in particular to a kind of lactobacillus-fermented preparation and its activation method.
Background technique
Microorganism live bacteria preparation refers to that with specific (beneficial) microorganism live bacteria quantity (i.e. effective bacterium number) be quality index
Microorganism live bacteria product, can be used as feedstuff leavening or feed micro-ecological additive apply ensiling, biological fermentation feed,
In the cultivation industries such as the soaked health product of liquid state fermentation feed, livestock and poultry.Presently commercially available microorganism live bacteria formulation products form has
Liquid formulation and two kinds of dry powder formulation.
The transportation cost of liquid formulation is high, and the high water activity (aw) of liquid formulation can accelerate the new of microorganism in storage
Old metabolism and activity decay cause thallus death, effective bacterium number to reduce, keep fermentation or using effect unstable, therefore liquid formulation
The accounting very little in active bacteria formulation product.
The lower metabolic activity that can inhibit microorganism of the water activity of dry powder formulation is conducive to save effective bacterium number, but
The disadvantage is that the microbial metabolic activity 1. in dry powder formulation is lower, ensiling or feedstuff are directly inoculated with without liquid activation
Substrate ferments, and the growth delay phase of microorganism is longer, and increasing time cost and being unfavorable for rapidly developing is dominant strain, shadow
Ring fermentation quality；2. 95% or more solid content is carrier in dry powder, pipeline is easily caused when largely using such cut-back product
Blocking and water quality burden, the applied defect of this especially soaked health product.
Liquid formulation and dry powder formulation have that microbial activity is unstable when application.It is being sent out to improve it
Microbial activity when ferment and use, certain products make activation operation instruction and (content are placed in clear water and activates one
Section time, or be properly added white sugar, molasses or salt again etc.), although activation step is easy, whether succeed and activation effect such as
He Jun is unknowable, unless having professional microbial fermentation experience personage, otherwise user can not be recognized.Current activation side
Method still cannot preferably solve active bacteria formulation microbial activity instability problem.
Summary of the invention
In view of this, the present invention is directed to propose a kind of lactobacillus-fermented preparation and its activation method, to solve active bacteria formulation
Microbial activity instability problem.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
A kind of lactobacillus-fermented preparation is made of lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protective agent.
Further, the lactic acid bacteria culturers be lactobacillus plantarum, Lactobacillus rhamnosus, enterococcus faecium, lactobacillus acidophilus,
Pediococcus acidilactici, streptococcus thermophilus, Lactobacillus salivarius, lactobacillus fermenti, lactobacillus reuteri, lactobacillus buchneri, cheese cream bar
One or more of bacterium, lactobacillus bulgaricus, Lactobacillus delbrueckii, Rui Shi lactobacillus, lactobacillus lactis and Pediococcus pentosaceus,
And viable count of lactobacillus > 2 × 10 in every 100g lactobacillus-fermented preparation10CFU。
Further, the Nutrious fermented object includes carbon source and nitrogen source, and the carbon source is glucose, sucrose, lactose, malt
One or more of sugar, soluble starch, galactolipin, sodium citrate, sodium succinate, sodium acetate, sodium succinate, and lactic acid bacteria
Carbon source accounts for the 40%-65% of lactobacillus-fermented preparation gross mass in fermentation preparation；The nitrogen source includes yeast extract, beef leaching
One or more of powder, tryptone, Yeast protein peptone, casein, soya peptone, ammonium acetate, ammonium chloride, ammonium sulfate, and cream
Nitrogen source accounts for the 30%-45% of lactobacillus-fermented preparation gross mass in acid bacteria fermentation preparation, and the Nutrious fermented object is raw for lactic acid bacteria
It is long that nutrition is provided.
Further, the product control indicator be one of reindeer moss, bromocresol purple, methyl orange, bromothymol blue or
It is several, and product control indicator accounts for the 0.1%-1.0% of lactobacillus-fermented preparation gross mass, the product control in lactobacillus-fermented preparation
Indicator indicates lactic bacteria activity and bacterium number product control.
Further, the protective agent is whey powder, skimmed milk power, trehalose, sucrose, oligofructose, cornstarch, paste
One or more of essence, and protective agent accounts for the 1%-14% of lactobacillus-fermented preparation gross mass, institute in lactobacillus-fermented preparation
It states protective agent and resists environmental change, protect lactobacillus cell structural integrity, maintain lactic acid bacteria fermenting agent to be in low water activity, prevent
Lactic acid bacteria metabolism decline is dead.
The activation method of above-mentioned lactobacillus-fermented preparation, comprising the following steps:
1) by lactobacillus-fermented preparation diluent into clear water, wherein the mass ratio of lactobacillus-fermented preparation and clear water is 1:20
~1:30 weighs the weight of lactobacillus-fermented preparation first, then weighs clear water in proportion, and by lactobacillus-fermented preparation and clearly
Water pours into bucket type container, covers container cover, is uniformly mixed lactobacillus-fermented preparation with clear water, and mixing liquid color is at this time
Purple；The bucket type container is transparent material, facilitates color change in observation container, is equipped with container above the bucket type container
Lid；
2) above-mentioned mixing liquid is placed naturally in 15~30 DEG C of room temperature and is activated, visually observe solution colour become it is red,
Activation terminates when one of yellow, orange, orange red, orange-yellow, khaki, and the lactic acid bacteria number for activating terminal is 2 × 108~
8×108CFU/mL obtains the lactobacillus-fermented preparation activating solution in growth logarithmic phase.
It can be 2~3L/ according to different demands, additive amount according to the lactobacillus-fermented preparation activating solution of above method preparation
Ton can be used for being inoculated with ensiling, biological fermentation feed or liquid state fermentation feeding system, can quickly form dominant strain, drop rapidly
Low material pH value inhibits miscellaneous bacteria and harmful bacteria growth, generates a large amount of lactic acid, material is made to have special sour smell, improves dynamic
Object palatability and growth performance；It can also be used for directly puddling feed feeding, spraying feeding.
Compared with the existing technology, a kind of lactobacillus-fermented preparation and its activation method of the present invention have following excellent
Gesture:
1. a kind of lactobacillus-fermented preparation of the present invention is low in dry powdery, transportation cost, and low water activity can press down
The metabolic activity of microorganism processed is conducive to save the effective bacterium number of lactic acid bacteria；2. containing lactobacillus inoculation and Nutrious fermented object, activation behaviour
Make simplicity, without equipment and energy consumption cost: lactobacillus-fermented preparation being diluted in clear water with the weight ratio of 1:20~1:30, in saturating
It is uniformly mixed after being covered in bright bucket type container, 15~30 DEG C of room temperature places activation naturally, without additionally adding other nutrition
Substance (carbon source, nitrogen source, growth factor etc.)；3. lactic bacteria activity and bacterium number product control are stablized, user's acceptable degree is high:
For activation terminal to visually observe the red judgement of solution colour flavescence, the lactic acid bacteria number for activating terminal is 2 × 108~8 × 108CFU/
ML solves active bacteria formulation microbial activity instability problem in growth logarithmic phase.
The high-activity lactic acid bacteria bacterium solution of a kind of lactobacillus-fermented preparation activation method preparation according to the present invention simultaneously
It is primarily useful for inoculation ensiling, biological fermentation feed or liquid state fermentation feeding system, dominant strain can be quickly formed, drop rapidly
Low material pH value inhibits the miscellaneous bacterias such as mould, saccharomycete and the growth of other harmful bacterias；At the same time, a large amount of lactic acid are generated, material is made
With special sour smell, animal palatability and growth performance are improved.Furthermore, it may also be used for directly puddle feed feeding, spray
Mist feeding etc..
Specific embodiment
In addition to being defined, technical term used in following embodiment has universal with those skilled in the art of the invention
The identical meanings of understanding.Test reagent used in following embodiment is unless otherwise specified conventional biochemical reagent；It is described
Experimental method is unless otherwise specified conventional method.The following examples are intended to illustrate the invention, but is not limited to this hair
Bright range.Bacterial strain used in the embodiment of the present invention is mainly bought from Chinese industrial Microbiological Culture Collection administrative center
(CICC) it can be obtained through commercial channels with Guangdong Province's Culture Collection (CIMCC), experiment reagent.
Below with reference to embodiment, the present invention will be described in detail.
Embodiment one
A kind of lactobacillus-fermented preparation total amount is 100g, by lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protection
Agent composition.
The lactic acid bacteria culturers are Lactobacillus rhamnosus 2 × 1010CFU, lactobacillus acidophilus 2 × 1010CFU。
The Nutrious fermented object includes carbon source and nitrogen source, and the carbon source is glucose 20%, sucrose 10%, lactose 10%,
Sodium citrate 12%, sodium acetate 3%；The nitrogen source is yeast extract 5%, beef extract powder 10%, soya peptone 20%, ammonium sulfate
5%, the Nutrious fermented object provides nutrition for lactobacter growth.
The product control indicator is bromocresol purple 0.2%, bromothymol blue 0.2%, the product control indicator instruction cream
Sour bacterium activity and bacterium number product control.
The protective agent is whey powder 3%, skimmed milk power 0.6%, cornstarch 1.0%, the protective agent resistance environment
Lactobacillus cell structural integrity is protected in variation, and lactic acid bacteria fermenting agent is maintained to be in low water activity, prevents lactic acid bacteria metabolism decline dead
It dies.
Wherein percentage is the mass percent for accounting for lactobacillus-fermented preparation total amount.
A kind of activation method of lactobacillus-fermented preparation, comprising the following steps:
1) by lactobacillus-fermented preparation diluent into clear water, wherein the mass ratio of lactobacillus-fermented preparation and clear water is 1:20
~1:30 weighs the weight of lactobacillus-fermented preparation first, then weighs clear water in proportion, and by lactobacillus-fermented preparation and clearly
Water pours into bucket type container, covers container cover, is uniformly mixed lactobacillus-fermented preparation with clear water, and mixing liquid color is at this time
Purple；The bucket type container is transparent material, facilitates color change in observation container, is equipped with container above the bucket type container
Lid；
2) above-mentioned mixing liquid is placed into activation 2-5 days at 20 DEG C of room temperature naturally, visually observing solution colour becomes orange
Or activation terminates when khaki, the lactic acid bacteria number for activating terminal is 2 × 108~8 × 108CFU/mL obtains lactobacillus-fermented preparation
Activating solution.
The lactobacillus-fermented preparation activating solution additive amount is 2~3L/ tons, can be used for being inoculated with ensiling, biofermentation is raised
Material or liquid state fermentation feed system, can quickly form dominant strain, reduce rapidly material pH value, inhibit miscellaneous bacteria and harmful bacteria raw
It is long, a large amount of lactic acid are generated, make material that there is special sour smell, improve animal palatability and growth performance；It can be used for
Directly puddle feed feeding, spraying feeding.
Embodiment two
A kind of lactobacillus-fermented preparation total amount is 200g, by lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protection
Agent composition.
The lactic acid bacteria culturers are lactobacillus plantarum 2 × 1010CFU, lactobacillus fermenti 3 × 1010CFU, Pediococcus acidilactici 3
×1010CFU, Lactobacillus casei 2 × 1010CFU。
The Nutrious fermented object includes carbon source and nitrogen source, and the carbon source is sucrose 15%, maltose 15%, soluble starch
15%, sodium succinate 4%；The nitrogen source is beef extract powder 20%, tryptone 10%, soya peptone 10%, ammonium acetate 5%, institute
It states Nutrious fermented object and provides nutrition for lactobacter growth.
The product control indicator is reindeer moss 0.6%, methyl orange 0.2%, product control indicator instruction lactic bacteria activity and
Bacterium number product control.
The protective agent is skimmed milk power 2.0%, cornstarch 1.2%, dextrin 2%, the protective agent resistance environment change
Change, protect lactobacillus cell structural integrity, lactic acid bacteria fermenting agent is maintained to be in low water activity, prevents lactic acid bacteria metabolism decline dead
It dies.
Wherein percentage is the mass percent for accounting for lactobacillus-fermented preparation total amount.
1) by lactobacillus-fermented preparation diluent into clear water, wherein the mass ratio of lactobacillus-fermented preparation and clear water is 1:20
~1:30 weighs the weight of lactobacillus-fermented preparation first, then weighs clear water in proportion, and by lactobacillus-fermented preparation and clearly
Water pours into bucket type container, covers container cover, is uniformly mixed lactobacillus-fermented preparation with clear water, and mixing liquid color is at this time
Purple；The bucket type container is transparent material, facilitates color change in observation container, is equipped with container above the bucket type container
Lid；
2) above-mentioned mixing liquid is placed into activation 2-5 days at 15 DEG C of room temperature naturally, visually observing solution colour becomes red
Or activation terminates when orange red, the lactic acid bacteria number for activating terminal is 2 × 108~8 × 108CFU/mL obtains lactobacillus-fermented preparation
Activating solution.
The lactobacillus-fermented preparation activating solution additive amount is 2~3L/ tons, can be used for being inoculated with ensiling, biofermentation is raised
Material or liquid state fermentation feed system, can quickly form dominant strain, reduce rapidly material pH value, inhibit miscellaneous bacteria and harmful bacteria raw
It is long, a large amount of lactic acid are generated, make material that there is special sour smell, improve animal palatability and growth performance；It can be used for
Directly puddle feed feeding, spraying feeding.
Embodiment three
A kind of lactobacillus-fermented preparation total amount is 500g, by lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protection
Agent composition.
The lactic acid bacteria culturers are lactobacillus bulgaricus 2 × 1010CFU, lactobacillus plantarum 2 × 1010CFU, enterococcus faecium
2×1010CFU, lactobacillus reuteri 2 × 1010CFU。
The Nutrious fermented object includes carbon source and nitrogen source, and the carbon source is glucose 25%, galactolipin 10%, sodium succinate
2%, sodium succinate 8%；The nitrogen source is beef extract powder 20%, Yeast protein peptone 15%, ammonium acetate 4%, ammonium chloride 4%, institute
It states Nutrious fermented object and provides nutrition for lactobacter growth.
The product control indicator is bromocresol purple 0.1%, methyl orange 0.1%, bromothymol blue 0.1%, the product control
Indicator indicates lactic bacteria activity and bacterium number product control.
The protective agent is skimmed milk power 5%, trehalose 1.2%, oligofructose 0.5%, dextrin 5%, the protective agent
Environmental change is resisted, lactobacillus cell structural integrity is protected, maintains lactic acid bacteria fermenting agent to be in low water activity, prevents lactic acid bacteria generation
It is dead to thank to decline.
Wherein percentage is the mass percent for accounting for lactobacillus-fermented preparation total amount.
A kind of activation method of lactobacillus-fermented preparation, comprising the following steps:
1) by lactobacillus-fermented preparation diluent into clear water, wherein the mass ratio of lactobacillus-fermented preparation and clear water is 1:20
~1:30 weighs the weight of lactobacillus-fermented preparation first, then weighs clear water in proportion, and by lactobacillus-fermented preparation and clearly
Water pours into bucket type container, covers container cover, is uniformly mixed lactobacillus-fermented preparation with clear water, and mixing liquid color is at this time
Purple；The bucket type container is transparent material, facilitates color change in observation container, is equipped with container above the bucket type container
Lid；
2) above-mentioned mixing liquid is placed into activation 2-5 days at 20 DEG C of room temperature naturally, visually observing solution colour becomes yellow
Or activation terminates when orange-yellow, the lactic acid bacteria number for activating terminal is 2 × 108~8 × 108CFU/mL obtains lactobacillus-fermented preparation
Activating solution.
The lactobacillus-fermented preparation activating solution additive amount is 2~3L/ tons, can be used for being inoculated with ensiling, biofermentation is raised
Material or liquid state fermentation feed system, can quickly form dominant strain, reduce rapidly material pH value, inhibit miscellaneous bacteria and harmful bacteria raw
It is long, a large amount of lactic acid are generated, make material that there is special sour smell, improve animal palatability and growth performance；It can be used for
Directly puddle feed feeding, spraying feeding.
Lactobacillus-fermented preparation provided by the invention is diluted in clear water with the weight ratio of 1:20~1:30, room temperature 15~
30 DEG C of activation, through test of many times, when solution colour is become from purple red, yellow, in orange, orange red, orange-yellow, khaki
One kind, detecting lactic acid bacteria number (according to national standard GB4789.35-2010) in solution is 2 × 108~8 × 108CFU/mL is in
The growth logarithmic phase of lactic acid bacteria shows that the product lactic bacteria activity and bacterium number product control are stablized.
Lactobacillus-fermented dry powder formulations in the present invention can be kept dry 12 months in room temperature cool place, and activation act letter
Just, without equipment and energy consumption cost, user's acceptable degree is high, and small-sized and scale livestock farming, feed factory can be used.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of lactobacillus-fermented preparation, it is characterised in that: by lactic acid bacteria culturers, Nutrious fermented object, product control indicator and protection
Agent composition.
2. a kind of lactobacillus-fermented preparation according to claim 1, it is characterised in that: the lactic acid bacteria culturers are plant cream
Bacillus, Lactobacillus rhamnosus, enterococcus faecium, lactobacillus acidophilus, Pediococcus acidilactici, streptococcus thermophilus, Lactobacillus salivarius, acidified milk
Bacillus, lactobacillus reuteri, lactobacillus buchneri, Lactobacillus casei, lactobacillus bulgaricus, Lactobacillus delbrueckii, Rui Shi lactobacillus,
One or more of lactobacillus lactis and Pediococcus pentosaceus, and viable count of lactobacillus > 2 in every 100g lactobacillus-fermented preparation
×1010CFU。
3. a kind of lactobacillus-fermented preparation according to claim 1, it is characterised in that: the Nutrious fermented object includes carbon source
And nitrogen source, the carbon source be glucose, sucrose, lactose, maltose, soluble starch, galactolipin, sodium citrate, sodium succinate,
One or more of sodium acetate, sodium succinate, and carbon source accounts for lactobacillus-fermented preparation gross mass in lactobacillus-fermented preparation
40%-65%；The nitrogen source includes yeast extract, beef extract powder, tryptone, Yeast protein peptone, casein, soya peptone, vinegar
One or more of sour ammonium, ammonium chloride, ammonium sulfate, and nitrogen source accounts for lactobacillus-fermented preparation gross mass in lactobacillus-fermented preparation
30%-45%.
4. a kind of lactobacillus-fermented preparation according to claim 1, it is characterised in that: the product control indicator be reindeer moss,
One or more of bromocresol purple, methyl orange, bromothymol blue, and product control indicator accounts for lactic acid in lactobacillus-fermented preparation
The 0.1%-1.0% of bacterium fermentation preparation gross mass.
5. a kind of lactobacillus-fermented preparation according to claim 1, it is characterised in that: the protective agent is whey powder, takes off
One or more of rouge milk powder, trehalose, sucrose, oligofructose, cornstarch, dextrin, and protected in lactobacillus-fermented preparation
Shield agent accounts for the 1%-14% of lactobacillus-fermented preparation gross mass.
6. a kind of activation method of lactobacillus-fermented preparation according to claim 1-5, it is characterised in that: including
Following steps:
1) by lactobacillus-fermented preparation diluent into clear water, wherein the mass ratio of lactobacillus-fermented preparation and clear water is 1:20~1:
30, the weight of lactobacillus-fermented preparation is weighed first, then weighs clear water in proportion, and lactobacillus-fermented preparation and clear water are fallen
Enter in bucket type container, cover container cover, be uniformly mixed lactobacillus-fermented preparation with clear water, mixing liquid color is purple at this time
Color；The bucket type container is transparent material, facilitates color change in observation container, is equipped with container cover above the bucket type container；
2) above-mentioned mixing liquid is placed naturally in 15~30 DEG C of room temperature and is activated, visually observe solution colour become red, yellow,
Activation terminates when one of orange, orange red, orange-yellow, khaki, and the lactic acid bacteria number for activating terminal is 2 × 108~8 ×
108CFU/mL obtains the lactobacillus-fermented preparation activating solution in growth logarithmic phase.
7. a kind of activation method of lactobacillus-fermented preparation according to claim 6, it is characterised in that: the lactic acid bacteria hair
Ferment preparation activating solution feeds system for being inoculated with ensiling, biological fermentation feed or liquid state fermentation, can quickly form dominant strain,
Material pH value is reduced rapidly, inhibits miscellaneous bacteria and harmful bacteria growth, generates a large amount of lactic acid, make material that there is special sour smell,
Improve animal palatability and growth performance；The lactobacillus-fermented preparation activating solution is for directly puddling feed feeding, spraying feeding
Hello.
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