CN109666597A - A kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed - Google Patents
A kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed Download PDFInfo
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Abstract
The present invention relates to biological field, specifically a kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed.A kind of complex microorganism inorganic agent of fermentation ensiling Huang storage feed, it is characterized in that being made of the material of following parts by weight: 10~30 parts of lactobacillus plantarum, 15~30 parts of bacillus coagulans, 15~30 parts of Lactobacillus rhamnosus, 10~30 parts of lactobacillus buchneri, the living bacteria count of the microbiological garbage treatment agent are 1.0~9.9 × 109CFU/mL.The present invention is compared with the existing technology, facultative aerobic bacillus coagulans and lactobacillus plantarum, Lactobacillus rhamnosus and lactobacillus buchneri complex micro organism fungicide are used for ensiling/Huang storage fermentation, pH can near 3.7~4.1 after varied bacteria growing breeding, fermentation can be effectively suppressed, improve organic acid total content acetic acid, propionic acid and butyric acid proportion, reduce ammonia nitrogen content, there is finished product fragrant fruit flavor to improve the nutritive value of feed to reduce the mouthfeel for being conducive to improve feed.
Description
Technical field
The present invention relates to biological field, the complex microorganism inorganic agent of specifically a kind of fermentation ensiling Huang storage feed and
Preparation method.
Background technique
China generates a large amount of agricultural crop straws as large agricultural country every year, such as a large amount of wheat, corn, beans and sorghum
Equal stalks, these only a small amount of roughages store as ensiling/Huang as animal productiong.World Developed Countries basically form " straw
The fertilizer regime of stalk+barnyard manure+chemical fertilizer ", China have completely forbidden crop straw burning returning to the field, and many stalks directly rot to make in field
It crushes for base manure or stalk as production organic fertilizer auxiliary material, but overall utilization rate is low.And China's ensiling in recent years/Huang storage is raised
The problems such as material demand is increasingly proliferated, and many still continues more original natural ensiling at present, and ensilage is mouldy, secondary fermentation is tight
Weight, some cattle farms even feed the ensilage for covering with mycotic spore, and feeding moldy metamorphism blueness leads to the case of dynamic bin stock object death
Example is countless.It can be seen that the quality of China's ensilage is in urgent need to be improved.
Ensiling/Huang storage be respectively by fresh (blueness) stalk or dried out the materials such as (Huang) stalk after centainly crushing and
Water and microbial bacterial agent or enzyme under suitable damp condition, are fermented, generally by being pressed and sealed using microorganism
Aerobic fermentation phase early period, anaerobic acidification decomposed phase need to be undergone and stablize storage life, acted on through microbial fermentation, it will be in ensiling raw material
The carbohydrate such as carbohydrate become the organic acids such as the lactic acid easily absorbed, improve the flavor and quality of feed, and lower pH inhibits mould
The growth of the miscellaneous bacterias such as bacterium, to extend the preservation of ensiling.Bacillus coagulans belong to facultative anaerobic bacteria in the present invention, can accelerate
In aerobic fermentation stage early period, rapidly enter anaerobic fermentation stage, lactobacillus plantarum, Lactobacillus rhamnosus and lactobacillus buchneri and solidifying
Knot accelerates anaerobic fermentation in anaerobic stages coordinative role, shortens fermentation period.Research shows that: ensiling/Huang stores feed not after fermentation
Any antibiotic is added, and rich in fermentable fiber element lactic acid, calcium lactate, small peptide, amino acid, vitamin and probiotics etc., easily
Digestion, the features such as nutritive value is higher, palatability is good, and animal digestive system Bacterial community can be improved, reduce digestive system disease
Disease occurs.
Summary of the invention
The present invention in order to overcome the deficiencies of the prior art, by the plant cream bar of the bacillus coagulans of amphimicrobian and anaerobism
Bacterium, Lactobacillus rhamnosus and lactobacillus buchneri complex micro organism fungicide provide a kind of ensiling/Huang storage for ensiling/Huang storage fermentation
Effect it is good and stablize, effectively inhibit varied bacteria growing breeding, fermentation after pH can near 3.7~4.1, improve organic acid in lactic acid, second
Acid and propionic acid proportion reduce butyric acid and ammonia nitrogen content, and finished product has fragrant fruit flavor, to be conducive to improve feed
Mouthfeel improves the nutritive value of feed.
To achieve the above object, a kind of complex microorganism inorganic agent of fermentation ensiling Huang storage feed is designed, it is characterized in that by
The material of following parts by weight is made: 10~30 parts of lactobacillus plantarum, 15~30 parts of bacillus coagulans, Lactobacillus rhamnosus 15
~30 parts, 10~30 parts of lactobacillus buchneri, the living bacteria count of the microbiological garbage treatment agent are 1.0~9.9 × 109CFU/mL。
It is the lactobacillus plantarum strain of CCTCC NO:M2013690 that the lactobacillus plantarum, which preferentially uses deposit number, described
Bacillus coagulans are any one plant bacterial strains for being accredited as bacillus coagulans, and the Lactobacillus rhamnosus is any one plant
It is accredited as the bacterial strain of Lactobacillus rhamnosus, the lactobacillus buchneri is any one plant bacterium for being accredited as lactobacillus buchneri
Strain.
A kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed, it is characterized in that including the following steps:
S1 will be condensed by lactobacillus plantarum, Lactobacillus rhamnosus and lactobacillus buchneri respectively in respective MRS solid medium culture
Bacillus is inoculated in YPD solid medium；S2 is planting lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus buchneri in MRS
It is cultivated in sub- liquid fermentation medium MRS shaking flask, bacillus coagulans is carried out in liquid fermentation culture medium YPD
Culture；S3 respectively connects the seed liquor of lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus buchneri and bacillus coagulans respectively
Kind is cultivated in the fermenter, and single liquid microbe microbial inoculum is made；Single liquid microbe microbial inoculum is mixed, is obtained by S4
Complex microorganism inorganic agent；Wherein the condition of culture of MRS solid medium and YPD solid medium is respectively temperature 35 DEG C~37
DEG C and 40 DEG C~45 DEG C, incubation time is 17h~for 24 hours；The condition of culture of MRS fluid nutrient medium and YPD fluid nutrient medium point
Wei not be 35 DEG C~37 DEG C and 40 DEG C~45 DEG C of temperature, stationary culture is 17h~be for 24 hours 17h with 200r/min shaken cultivation
~for 24 hours.
MRS solid culture based formulas in the step S1 are as follows: peptone 10.0g, beef extract 10.0g, yeast powder 5.0g,
Glucose 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, Tween 80 1.0g, K2HPO42.0g, MgSO4·7H2O
0.2g, MnSO4·H2O 0.05g, CaCO320.0g, agar powder 15.0 g, distilled water 1.0L, tune pH to 6.6 when culture~
6.8。
YPD solid culture based formulas in the step S1 are as follows: yeast extract 10.0g, peptone 20.0g, glucose
20.0g, agar powder 15.0g, distilled water 1.0L, tune pH to 7.0 when culture.
The formula of the step S2 bacillus coagulans liquid fermentation medium YPD is glucose 1%~1.5%, albumen
Peptone 1%~1.5%, yeast extract 0.5%~1%, calcium chloride 0.5%~1%, K2HPO40.2%~0.5%, MgSO4·
7H2O 0.005%~0.01%, MnSO4·H2O 0.005%~0.01%, tune pH to 7.0 when fermentation；The plant cream bar
The formula of bacterium, Lactobacillus rhamnosus and lactobacillus buchneri liquid fermentation medium MRS is peptone 1%~2%, beef extract powder
1%~2%, yeast extract 0.5%~1.5%, glucose 1%~2%, sodium acetate 0.5%~1%, dibasic ammonium citrate
0.2%~0.5%, Tween 80 0.01%, K2HPO40.1%~0.5%, MgSO4·7H2O 0.05%~0.1%,
MnSO4·H2O 0.005%~0.01%, CaCO30.2%~0.5%, tune pH to 6.8 when fermentation.
The condition of culture of fermentor in the step S3 are as follows: 40 DEG C~45 DEG C of the temperature of the bacillus coagulans, stirring
150~200r/min of speed, initial pH 7.0, fermentation process does not control pH, passes through air filtering, dissolved oxygen >=30%, tank pressure
0.04~0.06MPa, incubation time 17h~for 24 hours；The object lactobacillus, Lactobacillus rhamnosus and (35 DEG C of temperature of lactobacillus buchneri
~40 DEG C, mixing speed 50r/min~100r/min, initial pH 6.8, fermentation process does not control pH, stuffiness, tank pressure 0.04
~0.06MPa, incubation time 10h~20h.
A kind of preparation method of fermentation ensiling Huang storage feed, it is characterized in that including the following steps: (1) by agricultural residues powder
It is broken to 2~3cm, and controls smashed stalk water content 65~75%；(2), composite microbial is added to smashed stalk
Object inorganic agent, additive amount are 200~400mL/ tons, and when the complex microorganism inorganic agent adds, every 200mL is watered 2kg；(3),
It after addition, is tied up using bundling coating machine, and the material tied up is fermented 20~40 in the case where 20~35 DEG C
It, finally obtains ensiling Huang storage feed.
The addition manner of complex microorganism inorganic agent is atomized spray in the step (2)；The pH of the ensilage
3.7~4.1, finished product has fragrant fruit flavor.
The present invention compared with the existing technology, by facultative aerobic bacillus coagulans and lactobacillus plantarum, rhamnose cream bar
PH after varied bacteria growing breeding, fermentation can be effectively suppressed for ensiling/Huang storage fermentation in bacterium and lactobacillus buchneri complex micro organism fungicide
Can near 3.7~4.1, improve organic acid total content acetic acid, propionic acid and butyric acid proportion, reduce ammonia nitrogen content, finished product tool
There is fragrant fruit flavor, to reduce the mouthfeel for being conducive to improve feed, improves the nutritive value of feed.
Specific embodiment
The present invention is in use, ensiling/Huang storage feed complex micro organism fungicide can be used for the blueness of agricultural crop straw
Storage/Huang storage, wherein agricultural crop straw includes: corn stover, beanstalk stalk, Wheat Straw, rice straw, cassava stalk and broomcorn straw
One or more of.
Embodiment 1:
Living bacteria count is 5.6 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 30 parts of lactobacillus plantarums,
20 parts of bacillus coagulans, 30 parts of Lactobacillus rhamnosus and 20 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of ensiling used is corn complete stool stalk, by crops stalk crushing to 2~3cm, controls material
The dosage of water content 65%, ensiling per ton/Huang storage material is 250mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms,
With in atomizers spray to ensiling/Huang storage material, tied up tightly with bundling coating machine, material moisture ferments at 33~35 DEG C
26 days, obtain ensilage.
Embodiment 2:
Living bacteria count is 8.5 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 30 parts of lactobacillus plantarums,
30 parts of bacillus coagulans, 20 parts of Lactobacillus rhamnosus and 20 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of ensiling used is corn complete stool stalk, by crops stalk crushing to 2~3cm, controls material
The dosage of water content 60%, ensiling per ton/Huang storage material is 300mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms,
With in atomizers spray to ensiling/Huang storage material, tied up tightly with bundling coating machine, material moisture ferments at 30~35 DEG C
28 days, obtain ensilage.
Embodiment 3:
Living bacteria count is 7.6 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 25 parts of lactobacillus plantarums,
30 parts of bacillus coagulans, 20 parts of Lactobacillus rhamnosus and 25 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of ensiling used is wheat stalk, and by crops stalk crushing to 2~3cm, it is aqueous to control material
The dosage of amount 60%, ensiling per ton/Huang storage material is 300mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms, with spray
Day with fog is sprayed onto the material of ensiling/Huang storage, is tied up tightly with bundling coating machine, and material moisture ferments 32 at 28~33 DEG C
It, obtains ensilage.
Embodiment 4:
Living bacteria count is 4.8 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 25 parts of lactobacillus plantarums,
25 parts of bacillus coagulans, 25 parts of Lactobacillus rhamnosus and 25 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of ensiling used is wheat stalk, and by crops stalk crushing to 2~3cm, it is aqueous to control material
The dosage of amount 60%, ensiling per ton/Huang storage material is 300mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms, with spray
Day with fog is sprayed onto the material of ensiling/Huang storage, is tied up tightly with bundling coating machine, and material moisture ferments 35 at 25~30 DEG C
It, obtains ensilage.
Embodiment 5:
Living bacteria count is 7.6 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 30 parts of lactobacillus plantarums,
20 parts of bacillus coagulans, 25 parts of Lactobacillus rhamnosus and 25 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of ensiling used is soybean stalk, and by crops stalk crushing to 2~3cm, it is aqueous to control material
The dosage of amount 60%, ensiling per ton/Huang storage material is 400mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms, with spray
Day with fog is sprayed onto the material of ensiling/Huang storage, is tied up tightly with bundling coating machine, and material moisture ferments 35 at 23~32 DEG C
It, obtains ensilage.
Embodiment 6:
Living bacteria count is 6.3 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 30 parts of lactobacillus plantarums,
25 parts of bacillus coagulans, 20 parts of Lactobacillus rhamnosus and 25 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of yellow storage used is corn complete stool stalk, by crops stalk crushing to 2~3cm, controls material
The dosage of water content 65%, ensiling per ton/Huang storage material is 350mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms,
With in atomizers spray to ensiling/Huang storage material, tied up tightly with bundling coating machine, material moisture ferments at 25~32 DEG C
38 days, obtain yellow storage feed.
Embodiment 7:
Living bacteria count is 8.6 × 10 after the microbial bacterial agent fermentation9CFU/mL, including 30 parts of lactobacillus plantarums,
30 parts of bacillus coagulans, 20 parts of Lactobacillus rhamnosus and 20 parts of lactobacillus buchneris, in parts by weight.
The agricultural crop straw of yellow storage used is wheat stalk, and by crops stalk crushing to 2~3cm, it is aqueous to control material
The dosage of amount 65%, ensiling per ton/Huang storage material is 350mL, and the every 200mL of complex micro organism fungicide is watered 2 kilograms, with spray
Day with fog is sprayed onto the material of ensiling/Huang storage, is tied up tightly with bundling coating machine, and material moisture ferments 40 at 25~32 DEG C
It, obtains yellow storage feed.
Ensiling/Huang stores feed results of sensory evaluation in 1 example of table
Wherein control group 1~8 is not added with any strain, and fermentation relies primarily on the self-contained strain of raw material.
Ensiling/Huang stores feeding quality evaluation result in 2 example of table
Wherein ND expression is not detected.
Ensiling/Huang stores feed microbe changes of contents in 3 example of table
Wherein ND expression is not detected.
To sum up, from results of sensory evaluation it can be seen that fermented in control group using self-contained strain, final face
Color is dirty-green or yellowish-brown, and test group is yellow green and glossy；Control group is pungent tart flavour, musty partially occurs, is tried
Test group for fragrant fruity, smell is soft, not pungent, tart flavour is dense；Quality test group is loosely soft, slightly wet, tack-free, and cauline leaf is protected
Hold original state, can be easily separated, and control group pinch it is agglomerating after be not easy to scatter.Therefore, test group results of sensory evaluation is relatively better than control
Group.
From quality assessment results it can be seen that；The pH value of control group is above 4.1, and the pH value of test group is 3.7~4.1；
The content of the lactic acid of test group siccative, acetic acid and propionic acid is above control group, and the content of butyric acid and ammoniacal nitrogen significantly lower than pair
According to the content value of group.Therefore, the quality assessment results of test group are better than control group.
From microbial flora content results it can be seen that the lactic acid content of test group is higher than 1~2 order of magnitude of control group；Examination
Group is tested almost without mould is detected, saccharomycete is not detected, Escherichia coli and Miyarisan within the scope of normal national standard, and
Mould, saccharomycete, Escherichia coli and the butyric acid bacterial content of control group are apparently higher than the content of test group.Therefore, test group is micro-
Biological flora content results are superior to control group.
Lactobacillus plantarum deposit number used in the present invention is CCTCC NO:M 2013690, the title of the lactobacillus plantarum
For lactobacillus plantarum Lp-G18 (Lactobacillus plantarum Lp-G18), depositary institution is Chinese Typical Representative culture guarantor
Hiding center, address are Wuhan, China Wuhan University, and the deposit date is on December 23rd, 2013.
Claims (9)
1. a kind of complex microorganism inorganic agent of fermentation ensiling Huang storage feed, it is characterized in that by the material system of following parts by weight
At: 10~30 parts of lactobacillus plantarum, 15~30 parts of bacillus coagulans, 15~30 parts of Lactobacillus rhamnosus, lactobacillus buchneri 10
~30 parts, the living bacteria count of the microbiological garbage treatment agent is 1.0~9.9 × 109CFU/mL。
2. a kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed according to claim 1,
It is characterized in that: it is the lactobacillus plantarum strain of CCTCC NO:M 2013690, institute that the lactobacillus plantarum, which preferentially uses deposit number,
Stating bacillus coagulans is any one plant bacterial strain for being accredited as bacillus coagulans, and the Lactobacillus rhamnosus is any one
Strain has been accredited as the bacterial strain of Lactobacillus rhamnosus, and the lactobacillus buchneri is any one plant and has been accredited as lactobacillus buchneri
Bacterial strain.
3. a kind of a kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed according to claim 1,
It is characterized in that including the following steps: S1, by lactobacillus plantarum, Lactobacillus rhamnosus and lactobacillus buchneri respectively in respective MRS
Bacillus coagulans are inoculated in YPD solid medium by solid medium culture；S2, by lactobacillus plantarum, rhamnose cream bar
Bacterium, lactobacillus buchneri are cultivated in liquid fermentation culture medium MRS shaking flask in MRS, by bacillus coagulans in seed
It is cultivated in liquid fermentation medium YPD；S3, respectively by lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus buchneri and condensation
The seed liquor of bacillus is inoculated with respectively cultivates in the fermenter, and single liquid microbe microbial inoculum is made；S4, by single liquid
The mixing of body microbial bacterial agent, obtains complex microorganism inorganic agent；The wherein culture of MRS solid medium and YPD solid medium
Condition is respectively 35 DEG C~37 DEG C and 40 DEG C~45 DEG C of temperature, and incubation time is the h of 17 h~24；MRS fluid nutrient medium and
The condition of culture of YPD fluid nutrient medium is respectively 35 DEG C~37 DEG C and 40 DEG C~45 DEG C of temperature, and stationary culture is 17 h~24
H and 200 r/min shaken cultivations are the h of 17 h~24.
4. a kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed according to claim 3, special
Sign is the MRS solid culture based formulas in the step S1 are as follows: 10.0 g of peptone, 10.0 g of beef extract, yeast powder 5.0
G, 5.0 g of glucose, 5.0 g of sodium acetate, dibasic ammonium citrate 2.0 g, Tween 80 1.0g, K2HPO42.0 g,
MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, CaCO320.0 g, 15.0 g of agar powder, 1.0 L of distilled water, training
Tune pH to 6.6~6.8 when supporting.
5. a kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed according to claim 3, special
Sign is YPD solid culture based formulas in the step S1 are as follows: 10.0 g of yeast extract, 20.0 g of peptone, glucose 20.0
G, 15.0 g of agar powder, 1.0 L of distilled water, tune pH to 7.0 when culture.
6. a kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed according to claim 3, special
Sign be the step S2 bacillus coagulans liquid fermentation medium YPD formula be glucose 1%~1.5%, peptone 1%~
1.5%, yeast extract 0.5%~1%, calcium chloride 0.5%~1%, K2HPO4 0.2%~0.5%, MgSO4·7H2O 0.005%~
0.01%, MnSO4·H2O 0.005%~0.01%, tune pH to 7.0 when fermentation；The lactobacillus plantarum, Lactobacillus rhamnosus and
The formula of lactobacillus buchneri liquid fermentation medium MRS is peptone 1%~2%, beef extract powder 1%~2%, yeast extract 0.5%
~1.5%, glucose 1%~2%, sodium acetate 0.5%~1%, dibasic ammonium citrate 0.2%~0.5%, Tween 80 0.01%,
K2HPO40.1%~0.5%, MgSO4·7H2O 0.05%~0.1%, MnSO4·H2O 0.005%~0.01%, CaCO30.2%~
0.5%, tune pH to 6.8 when fermentation.
7. a kind of complex microorganism inorganic agent preparation method of fermentation ensiling Huang storage feed according to claim 3, special
Sign is the condition of culture of fermentor in the step S3 are as follows: 40 DEG C~45 DEG C of the temperature of the bacillus coagulans, mixing speed
150~200 r/min, initial pH 7.0, fermentation process does not control pH, by air filtering, dissolved oxygen >=30%, tank pressure 0.04~
0.06 MPa, incubation time 17 h~24 h；The object lactobacillus, Lactobacillus rhamnosus and lactobacillus buchneri (35 DEG C of temperature~
40 DEG C, mixing speed 50 r/min~100 r/min, initial pH 6.8, fermentation process does not control pH, stuffiness, tank pressure 0.04
~0.06 MPa, incubation time 10 h~20 h.
8. a kind of preparation method of a kind of fermentation ensiling Huang storage feed according to claims 1 to 7, it is characterized in that including such as
Lower step: (1) by crops stalk crushing to 2~3 cms, and smashed stalk water content is controlled 65~75%；(2), to
Smashed stalk adds complex microorganism inorganic agent, and additive amount is 200~400 mL/ tons, the complex microorganism inorganic agent
When addition, every 200 mL is watered 2kg；(3), it after addition, is tied up using bundling coating machine, and the material tied up is existed
It ferments 20~40 days in the case where 20~35 DEG C, finally obtains ensiling Huang storage feed.
9. a kind of preparation method of fermentation ensiling Huang storage feed of volume according to claim 8, it is characterized in that: the step (2)
The addition manner of middle complex microorganism inorganic agent is atomized spray；The pH 3.7~4.1 of the ensilage, finished product have fragrance
Fruit flavor.
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Cited By (3)
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| CN110591988A (en) * | 2019-11-01 | 2019-12-20 | 四川农业大学 | Lactobacillus rhamnosus753 and application thereof, silage additive and silage |
| CN114246257A (en) * | 2022-03-01 | 2022-03-29 | 山东健源生物科技有限公司 | Biological agent for improving fermentation quality of mixed silage and preparation method thereof |
| CN117778278A (en) * | 2024-02-27 | 2024-03-29 | 山东中科嘉亿生物工程有限公司 | Compound lactobacillus silage additive and preparation method and application thereof |
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| CN108330080A (en) * | 2017-11-14 | 2018-07-27 | 北京好实沃生物技术有限公司 | A kind of feed ensiling agent and its preparation method and application containing lactobacillus buchneri |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110591988A (en) * | 2019-11-01 | 2019-12-20 | 四川农业大学 | Lactobacillus rhamnosus753 and application thereof, silage additive and silage |
| CN114246257A (en) * | 2022-03-01 | 2022-03-29 | 山东健源生物科技有限公司 | Biological agent for improving fermentation quality of mixed silage and preparation method thereof |
| CN117778278A (en) * | 2024-02-27 | 2024-03-29 | 山东中科嘉亿生物工程有限公司 | Compound lactobacillus silage additive and preparation method and application thereof |
| CN117778278B (en) * | 2024-02-27 | 2024-05-03 | 山东中科嘉亿生物工程有限公司 | Compound lactobacillus silage additive and preparation method and application thereof |
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