CN219174510U

CN219174510U - Lymphocyte culture device

Info

Publication number: CN219174510U
Application number: CN202222545513.3U
Authority: CN
Inventors: 程亚婷; 李颖; 汤浩; 杨国华; 陈志毅; 丁国庆; 黎妮
Original assignee: Wuhan Baiyi Biotechnology Co ltd
Current assignee: Wuhan Baiyi Biotechnology Co ltd
Priority date: 2022-09-26
Filing date: 2022-09-26
Publication date: 2023-06-13
Anticipated expiration: 2032-09-26

Abstract

The utility model discloses a lymphocyte culture device which comprises a lymphocyte culture box body, a vacuum seal box door, a support disc, a screw rod and a culture dish, wherein a sterilizing lamp and an illuminating lamp are arranged on the upper surface of the inside of the lymphocyte culture box body, a first conical gear is arranged below the screw rod, a first power motor is arranged on the left side of the lymphocyte culture box body, a second power motor is arranged below the lymphocyte culture box body, and the support disc is connected with a movable disc through a belt. This lymphocyte culture apparatus is provided with support disc, movable disk and belt, starts the second power motor, and the second power motor passes through the output shaft and drives the movable disk, and then the movable disk drives the support disc rotatory under the effect of belt, then the support disc drives the culture dish rotatory under the effect of dead lever and guide bar to be convenient for adjust culture dish angle, thereby can observe the lymphocyte of culture dish different positions.

Description

Lymphocyte culture device

Technical Field

The utility model relates to the technical field of lymphocyte culture, in particular to a lymphocyte culture device.

Background

Cell culture techniques refer to the growth of cells under in vitro conditions, cells no longer form tissues (animals) during the culture process, cultures are single cells or cell groups, cells need to be living in an artificial environment during the culture, the culture time is prolonged and the cells are passaged to be single due to the change of the environment or the influence of some other factors, and in the lymphocyte culture process, reagents, liquids, vessels and instruments contacted in a laboratory and in the culture process are strictly operated according to the requirements of reference books related to cell culture, the aseptic concept needs to run through the whole experiment, and the conventional lymphocyte culture device has the following problems:

1. when lymphocytes are cultured in the culture dish, the tightness of the inside of the culture device is required to be ensured, external bacteria are prevented from invading, the culture effect is affected, and the culture device with high tightness is inconvenient for an experimenter to observe the culture condition of the inside lymphocytes in time, and when the inside lymphocytes are observed outside, the observation angle is limited, so that the culture condition of the lymphocytes cannot be recorded accurately in time, and the experimental efficiency is affected.

We have therefore proposed a lymphocyte culture apparatus in order to solve the problems set out above.

Disclosure of Invention

The utility model aims to provide a lymphocyte culture device, which aims to solve the problems that when lymphocytes are cultured in a culture dish, the sealing performance of the inside of the culture device needs to be ensured, external bacteria are prevented from invading, the culture effect is influenced, the culture device with high sealing performance is inconvenient for an experimenter to observe the culture condition of the internal lymphocytes in time, and the observation angle is limited when the experimenter observes the internal lymphocytes outside, so that the culture condition of the lymphocytes cannot be recorded accurately in time, and the experiment efficiency is influenced.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a lymphocyte culture device, includes lymphocyte culture box, vacuum seal chamber door, support disc, lead screw and culture dish, the inside upper surface mounting of lymphocyte culture box has bactericidal lamp and light, and the light is located the central point of lymphocyte culture box inside upper surface put, the vacuum seal chamber door is installed to the positive surface of lymphocyte culture box, and the positive surface of vacuum seal chamber door is provided with the visual window, the cell fluid entry is installed in the left side of lymphocyte culture box, and the outside of cell fluid entry installs sealed lid, and cell fluid entry is close to inside one side of lymphocyte culture box and installs coupling hose, the support frame is installed to the inner bottom of lymphocyte culture box, and installs the support disc through the bearing piece in the top of support frame, and the inside axial of bearing piece is provided with the lead screw, bar nut is installed to the upper end of lead screw, and the top fixed mounting of nut has the culture dish, the below position of lead screw has first conical gear, the left side of lymphocyte culture box installs first power motor, and the right side of first output shaft motor installs the cell fluid entry, and cell fluid entry is close to one side of lymphocyte culture box installs coupling hose through the second conical gear, and the inside upper end is located the guide disc, the movable connection has the second through the guide disc, the movable connection has the movable output shaft on the movable disc, the movable connection has the movable disc.

Preferably, the wall thickness of the connecting hose is between 1mm and 2mm, the connecting hose is of an elastic structure design, and the connecting hose is positioned at the upper left side of the culture dish.

By adopting the structural design, when the lymphocyte is put into the lymphocyte culture box body from the cell sap inlet, the lymphocyte passes through the connecting hose, and the connecting hose is downwards dumped onto the culture dish under the action of the gravity of the lymphocyte, so that the lymphocyte is put into the culture dish, after the input is completed, the gravity born by the connecting hose disappears, and the connecting hose resets.

Preferably, the strip-shaped nut and the screw rod form a sliding structure, and the center lines of the strip-shaped nut and the screw rod are mutually overlapped with the center line of the culture dish.

By adopting the structural design, the strip-shaped nut can be driven to move up and down along the screw rod through the rotation of the screw rod, so that the culture dish above the strip-shaped nut is driven to move up and down, and the culture dish can be moved to a position convenient for observation.

Preferably, the first bevel gear and the second bevel gear are perpendicular to each other and meshed with each other, and the first bevel gear and the second bevel gear are located inside the supporting frame.

By adopting the structural design, the first power motor is started, the first power motor drives the second bevel gear to rotate through the output shaft, then the second bevel gear drives the first bevel gear, and then the first bevel gear drives the screw rod to rotate, so that power support is achieved for the rotation of the screw rod.

Preferably, the supporting disc and the movable disc are positioned on the same horizontal line, and the supporting disc and the movable disc are parallel to the bottom surface of the lymphocyte culture box body.

Adopt above-mentioned structural design, start the second motor power, the second motor power passes through the output shaft and drives the movable disk, and then the movable disk drives the support disc rotatory under the effect of belt, then the support disc drives the culture dish rotatory under the effect of dead lever and guide bar to be convenient for adjust culture dish angle, thereby can observe the lymphocyte of culture dish different positions.

Preferably, the fixing rod and the guide rod are provided with 2 groups, and the 2 groups of fixing rods and the guide rod are symmetrical to each other along the central line of the supporting disc, and the fixing rod is of a hollow structure design.

By adopting the structural design, when the screw rod drives the strip-shaped nut and the culture dish to move up and down, the guide rod below the culture dish moves up and down in the fixed rod, so that the up and down movement of the culture dish is limited and guided, and the culture dish is more stable to move.

Compared with the prior art, the utility model has the beneficial effects that: the lymphocyte culture apparatus comprises:

1. the movable disc is driven by the movable disc to rotate under the action of the belt, and the support disc drives the culture dish to rotate under the action of the fixed rod and the guide rod, so that the angle of the culture dish is convenient to adjust, and lymphocytes at different positions of the culture dish can be observed;

2. the device comprises a screw rod, a strip nut, a first conical gear and a second conical gear, wherein the first power motor is started, the first power motor drives the second conical gear to rotate through an output shaft, then the second conical gear drives the first conical gear, then the first conical gear drives the screw rod to rotate, then the screw rod drives the strip nut to move up and down along the screw rod, so that a culture dish above the strip nut is driven to move up and down, and the culture dish can be moved to a position convenient for observation;

3. the sterilizing lamp and the illuminating lamp are arranged, the sterilizing lamp can sterilize the internal environment of the lymphocyte incubator, the aseptic culture environment of lymphocytes is ensured, the illuminating lamp plays a role in illuminating the internal of the lymphocyte incubator, and the lymphocyte incubator is convenient to observe the lymphocytes;

4. the vacuum sealing box door and the visual window are arranged, the vacuum sealing box door is opened to facilitate overhauling and maintaining parts in the lymphocyte culture box body, and lymphocytes in a culture dish in the lymphocyte culture box body can be observed through the visual window on the vacuum sealing box door.

Drawings

FIG. 1 is a schematic diagram of a front view structure of the present utility model;

FIG. 2 is a schematic diagram of the main section structure of the present utility model;

FIG. 3 is an enlarged schematic view of the structure of FIG. 2A according to the present utility model;

FIG. 4 is an enlarged schematic view of the structure of FIG. 2B according to the present utility model;

FIG. 5 is a schematic diagram of the structure of the culture dish of the present utility model.

In the figure: 1. a lymphocyte incubator; 2. a germicidal lamp; 3. a lighting lamp; 4. vacuum sealing the door; 5. a visual window; 6. a cell fluid inlet; 7. sealing cover; 8. a connecting hose; 9. a support frame; 10. a bearing member; 11. a support disc; 12. a screw rod; 13. a bar nut; 14. a culture dish; 15. a first bevel gear; 16. a first power motor; 17. a second bevel gear; 18. a second power motor; 19. a movable plate; 20. a belt; 21. a fixed rod; 22. a guide rod.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-5, the present utility model provides a technical solution: a lymphocyte culture apparatus comprises a lymphocyte culture box body 1, a sterilizing lamp 2, an illuminating lamp 3, a vacuum seal box door 4, a visual window 5, a cell liquid inlet 6, a sealing cover 7, a connecting hose 8, a supporting frame 9, a bearing piece 10, a supporting disc 11, a screw rod 12, a bar nut 13, a culture dish 14, a first bevel gear 15, a first power motor 16, a second bevel gear 17, a second power motor 18, a movable disc 19, a belt 20, a fixed rod 21 and a guide rod 22, wherein the sterilizing lamp 2 and the illuminating lamp 3 are arranged on the upper surface of the inside of the lymphocyte culture box body 1, the illuminating lamp 3 is positioned at the central position of the upper surface of the inside of the lymphocyte culture box body 1, the vacuum seal box door 4 is arranged on the front surface of the lymphocyte culture box body 1, the visual window 5 is arranged on the front surface of the vacuum seal box door 4, the cell liquid inlet 6 is arranged on the left side of the lymphocyte culture box body 1, and the outside of the cell liquid inlet 6 is provided with a sealing cover 7, and one side of the cell liquid inlet 6 near the inside of the lymphocyte culture box body 1 is provided with a connecting hose 8, the inside bottom of the lymphocyte culture box body 1 is provided with a supporting frame 9, the upper part of the supporting frame 9 is provided with a supporting disc 11 through a bearing piece 10, the inside of the bearing piece 10 is axially provided with a screw rod 12, the upper end of the screw rod 12 is provided with a bar nut 13, the upper part of the bar nut 13 is fixedly provided with a culture dish 14, the wall thickness of the connecting hose 8 is 1mm, the connecting hose 8 is of an elastic structure design, and the connecting hose 8 is positioned at the upper left side of the culture dish 14, when the lymphocyte is put into the lymphocyte culture box body 1 from the cell liquid inlet 6, the lymphocyte passes through the connecting hose 8, the connecting hose 8 is downwards poured onto the culture dish 14 under the action of the gravity of the lymphocyte, thus, the lymphocytes are put into the culture dish 14, and after the completion of the putting, the gravity applied to the connection tube 8 disappears, and the connection tube 8 is reset.

The strip nut 13 and the screw rod 12 form a sliding structure, the center lines of the strip nut 13 and the screw rod 12 are mutually overlapped with the center line of the culture dish 14, the strip nut 13 can be driven to move up and down along the screw rod 12 through the rotation of the screw rod 12, thereby driving the culture dish 14 above the strip nut 13 to move up and down, further, the culture dish 14 can be moved to a position convenient for observation, a first conical gear 15 is arranged at the lower position of the screw rod 12, a first power motor 16 is arranged at the left side of the lymphocyte incubator body 1, a second conical gear 17 is arranged at the right side of the first power motor 16 through an output shaft, the first conical gear 15 and the second conical gear 17 are mutually perpendicular and mutually meshed, the first conical gear 15 and the second conical gear 17 are both positioned in the support frame 9, the first power motor 16 is started, the first power motor 16 drives the second conical gear 17 to rotate through the output shaft, then the second conical gear 17 drives the first conical gear 15, and then the first conical gear 15 drives the screw rod 12 to rotate, and thus the rotation of the screw rod 12 is supported.

The second power motor 18 is installed to lymphocyte culture box 1's below, and the top of second power motor 18 is through the output shaft mounting movable dish 19, support disc 11 and movable dish 19 are located same horizontal line, and support disc 11 and movable dish 19 are parallel to each other with lymphocyte culture box 1 bottom surface, start second power motor 18, second power motor 18 passes through the output shaft and drives movable dish 19, then movable dish 19 drives support disc 11 rotation under the effect of belt 20, then support disc 11 drives the culture dish 14 under the effect of dead lever 21 and guide bar 22 and rotates, thereby be convenient for adjust culture dish 14 angle, thereby can observe the lymphocyte of culture dish 14 different positions, and movable dish 19 is located the inside of lymphocyte culture box 1, be connected through belt 20 between support disc 11 and the movable dish 19, and support disc 11's top perpendicular installation dead lever 21, and the top swing joint of dead lever 21 has guide bar 22, dead lever 21 and guide bar 22 are provided with 2 groups, and 2 groups dead lever 21 and guide bar 22 are along the effect of supporting disc 11, and guide bar 21 and guide bar 22 are the effect of guide bar-shaped structure and make the guide bar-shaped upper end 14 and the fixed connection of guide bar-shaped upper and lower side 14 when the guide bar-shaped support disc 11 is the mutual, and guide bar-shaped upper end 14 moves down, thereby the fixed dish 14 moves under the guide bar-shaped upper end 14 and lower side 14 is fixed, the guide bar-shaped upper end of the guide bar-shaped support plate 12 is moved, and the guide bar-shaped upper end of the guide bar-plate 14 is moved, and lower bar-shaped upper end is more stable.

Working principle: when using this lymphocyte culture apparatus, first, the sealing cap 7 is opened, lymphocytes are put into the lymphocyte culture tank 1 through the cell fluid inlet 6, the lymphocytes are poured down onto the culture dish 14 through the connection tube 8 under the action of the lymphocyte gravity, and thus the lymphocytes are put into the culture dish 14, after the completion of the input, the gravity applied to the connection tube 8 disappears, and the connection tube 8 is reset.

When the lymphocytes in the culture dish 14 need to be observed, the first power motor 16 is started, the first power motor 16 drives the second bevel gear 17 to rotate through the output shaft, then the second bevel gear 17 drives the first bevel gear 15, then the first bevel gear 15 drives the screw rod 12 to rotate, then the screw rod 12 drives the bar nut 13 to move up and down along the screw rod 12, thereby driving the culture dish 14 above the bar nut 13 to move up and down, so that the culture dish 14 can be moved to a position convenient for observation, the second power motor 18 is started, the second power motor 18 drives the movable disc 19 through the output shaft, then the movable disc 19 drives the support disc 11 to rotate under the action of the belt 20, then the support disc 11 drives the culture dish 14 to rotate under the action of the fixed rod 21 and the guide rod 22, and therefore the angle of the culture dish 14 is convenient to adjust, and the lymphocytes in different positions of the culture dish 14 can be observed. Thereby completing a series of works. What is not described in detail in this specification is prior art known to those skilled in the art.

Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.

Claims

1. The utility model provides a lymphocyte culture device, includes lymphocyte culture box (1), vacuum seal chamber door (4), support disc (11), lead screw (12) and culture dish (14), its characterized in that: the utility model discloses a cell culture box, which is characterized in that a sterilizing lamp (2) and an illuminating lamp (3) are arranged on the inner upper surface of the cell culture box (1), the illuminating lamp (3) is positioned at the central position of the inner upper surface of the cell culture box (1), a vacuum seal box door (4) is arranged on the front surface of the cell culture box (1), a visual window (5) is arranged on the front surface of the vacuum seal box door (4), a cell fluid inlet (6) is arranged on the left side of the cell culture box (1), a sealing cover (7) is arranged on the outer side of the cell fluid inlet (6), a connecting hose (8) is arranged on one side of the cell fluid inlet (6) close to the inner side of the cell culture box (1), a supporting frame (9) is arranged on the inner bottom of the cell culture box (1), a supporting disc (11) is arranged above the supporting frame (9) through a bearing piece (10), a screw rod (12) is axially arranged in the inner side of the bearing piece (10), a nut (13) is arranged at the upper end of the screw rod (12), a cell fluid inlet (6) is arranged on the left side of the cell culture box (1), a sealing cover (7) is arranged on the outer side of the cell fluid inlet (6), a connecting hose (8) is arranged on one side of the cell culture box (1), a bar-shaped motor (16) is arranged on the inner side of the cell culture box (16), and second bevel gear (17) are installed through the output shaft on the right side of first power motor (16), second power motor (18) are installed to the below of lymphocyte incubator body (1), and movable dish (19) are installed through the output shaft to the top of second power motor (18), and movable dish (19) are located the inside of lymphocyte incubator body (1) moreover, be connected through belt (20) between support disc (11) and movable dish (19), and support the top of disc (11) and install dead lever (21) perpendicularly, and the top swing joint of dead lever (21) has guide bar (22) moreover, the upper end and the culture dish (14) bottom fixed connection of guide bar (22).

2. The lymphocyte culture apparatus according to claim 1, wherein: the wall thickness of the connecting hose (8) is between 1mm and 2mm, the connecting hose (8) is of elastic structure design, and the connecting hose (8) is positioned at the upper left side of the culture dish (14).

3. The lymphocyte culture apparatus according to claim 1, wherein: the strip-shaped nut (13) and the screw rod (12) form a sliding structure, and the central lines of the strip-shaped nut (13) and the screw rod (12) are mutually overlapped with the central line of the culture dish (14).

4. The lymphocyte culture apparatus according to claim 1, wherein: the first conical gear (15) and the second conical gear (17) are mutually perpendicular and meshed, and the first conical gear (15) and the second conical gear (17) are both positioned inside the supporting frame (9).

5. The lymphocyte culture apparatus according to claim 1, wherein: the supporting disc (11) and the movable disc (19) are positioned on the same horizontal line, and the supporting disc (11) and the movable disc (19) are parallel to the bottom surface of the lymphocyte incubator body (1).

6. The lymphocyte culture apparatus according to claim 1, wherein: the fixing rods (21) and the guide rods (22) are provided with 2 groups, the 2 groups of fixing rods (21) and the guide rods (22) are symmetrical to each other along the central line of the supporting disc (11), and the fixing rods (21) are of a hollow structure design.