CN109609565A - 一种发酵生产l-异亮氨酸的方法 - Google Patents
一种发酵生产l-异亮氨酸的方法 Download PDFInfo
- Publication number
- CN109609565A CN109609565A CN201910134832.5A CN201910134832A CN109609565A CN 109609565 A CN109609565 A CN 109609565A CN 201910134832 A CN201910134832 A CN 201910134832A CN 109609565 A CN109609565 A CN 109609565A
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- Prior art keywords
- isoleucine
- fermentation
- glucose
- medium
- dihydrogen phosphate
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 title claims abstract description 55
- 238000000855 fermentation Methods 0.000 claims abstract description 66
- 230000004151 fermentation Effects 0.000 claims abstract description 66
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 32
- 239000008103 glucose Substances 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 31
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 27
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- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 27
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims abstract description 23
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims abstract description 23
- 229960001230 asparagine Drugs 0.000 claims abstract description 23
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- 241000424760 Corynebacterium crenatum Species 0.000 claims abstract description 9
- 239000002609 medium Substances 0.000 claims description 46
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 44
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 22
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 21
- 235000019764 Soybean Meal Nutrition 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
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- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 6
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- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 5
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- 239000000908 ammonium hydroxide Substances 0.000 claims description 4
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- 239000004952 Polyamide Substances 0.000 claims description 3
- 229920002301 cellulose acetate Polymers 0.000 claims description 3
- 229920002647 polyamide Polymers 0.000 claims description 3
- 229920006393 polyether sulfone Polymers 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 2
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- 238000001291 vacuum drying Methods 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims 1
- 238000009423 ventilation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 229930182844 L-isoleucine Natural products 0.000 abstract description 9
- 229960000310 isoleucine Drugs 0.000 abstract description 9
- NAOLWIGVYRIGTP-UHFFFAOYSA-N 1,3,5-trihydroxyanthracene-9,10-dione Chemical compound C1=CC(O)=C2C(=O)C3=CC(O)=CC(O)=C3C(=O)C2=C1 NAOLWIGVYRIGTP-UHFFFAOYSA-N 0.000 abstract description 8
- 238000006243 chemical reaction Methods 0.000 abstract description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 7
- 229940024606 amino acid Drugs 0.000 abstract description 5
- 235000001014 amino acid Nutrition 0.000 abstract description 5
- 150000001413 amino acids Chemical class 0.000 abstract description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 239000002351 wastewater Substances 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 9
- 241000186226 Corynebacterium glutamicum Species 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 240000006365 Vitis vinifera Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 238000009776 industrial production Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 241000319304 [Brevibacterium] flavum Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
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- 238000005342 ion exchange Methods 0.000 description 2
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- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- AGPKZVBTJJNPAG-AKGZTFGVSA-N (2s)-2-amino-3-methylpentanoic acid Chemical compound CCC(C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-AKGZTFGVSA-N 0.000 description 1
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- XDDMZVMWZMSAMX-JHNJPSDUSA-N (2s,3s)-2-azanyl-3-methyl-pentanoic acid Chemical compound CC[C@H](C)[C@H](N)C(O)=O.CC[C@H](C)[C@H](N)C(O)=O XDDMZVMWZMSAMX-JHNJPSDUSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
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- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
- 229940025294 hemin Drugs 0.000 description 1
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- 150000002431 hydrogen Chemical class 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- 229910052700 potassium Inorganic materials 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110396493A (zh) * | 2019-09-09 | 2019-11-01 | 廊坊梅花生物技术开发有限公司 | 培养基组合及生产异亮氨酸的方法 |
CN114606275A (zh) * | 2022-02-11 | 2022-06-10 | 安徽丰原发酵技术工程研究有限公司 | 一种发酵生产l-异亮氨酸的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0548410A1 (en) * | 1991-12-23 | 1993-06-30 | Unilever N.V. | Process for producing edible matter having an enhanced flavour, a flavouring substance obtainable according to said process and the use of such a flavouring substance for imparting flavour to foodstuffs |
CN101899482A (zh) * | 2010-07-28 | 2010-12-01 | 无锡晶海氨基酸有限公司 | 一种降低l-异亮氨酸发酵废液中铵氮含量的方法 |
CN103409476A (zh) * | 2013-08-30 | 2013-11-27 | 北京明新高科技发展有限公司 | 一种l-异亮氨酸的发酵及分离纯化方法 |
CN105695526A (zh) * | 2016-04-06 | 2016-06-22 | 河南巨龙生物工程股份有限公司 | 一种通过分步补料提高l-缬氨酸产酸的发酵工艺 |
CN108796003A (zh) * | 2018-06-28 | 2018-11-13 | 无锡晶海氨基酸股份有限公司 | 一种提高l-异亮氨酸产量的方法 |
CN108841884A (zh) * | 2018-06-28 | 2018-11-20 | 无锡晶海氨基酸股份有限公司 | 一种高效生产l-异亮氨酸的方法 |
-
2019
- 2019-02-24 CN CN201910134832.5A patent/CN109609565B/zh active Active
Patent Citations (7)
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---|---|---|---|---|
EP0548410A1 (en) * | 1991-12-23 | 1993-06-30 | Unilever N.V. | Process for producing edible matter having an enhanced flavour, a flavouring substance obtainable according to said process and the use of such a flavouring substance for imparting flavour to foodstuffs |
CN101899482A (zh) * | 2010-07-28 | 2010-12-01 | 无锡晶海氨基酸有限公司 | 一种降低l-异亮氨酸发酵废液中铵氮含量的方法 |
CN103409476A (zh) * | 2013-08-30 | 2013-11-27 | 北京明新高科技发展有限公司 | 一种l-异亮氨酸的发酵及分离纯化方法 |
CN103409476B (zh) * | 2013-08-30 | 2017-12-26 | 中科医药行业生产力促进中心有限公司 | 一种l‑异亮氨酸的发酵及分离纯化方法 |
CN105695526A (zh) * | 2016-04-06 | 2016-06-22 | 河南巨龙生物工程股份有限公司 | 一种通过分步补料提高l-缬氨酸产酸的发酵工艺 |
CN108796003A (zh) * | 2018-06-28 | 2018-11-13 | 无锡晶海氨基酸股份有限公司 | 一种提高l-异亮氨酸产量的方法 |
CN108841884A (zh) * | 2018-06-28 | 2018-11-20 | 无锡晶海氨基酸股份有限公司 | 一种高效生产l-异亮氨酸的方法 |
Non-Patent Citations (2)
Title |
---|
刘勇: "《分批补料培养对L_异亮氨酸发酵的影响》", 《工业微生物》 * |
黄平: "《L-异亮氨酸生产菌发酵水平提高的研究》", 《化工设计通讯》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110396493A (zh) * | 2019-09-09 | 2019-11-01 | 廊坊梅花生物技术开发有限公司 | 培养基组合及生产异亮氨酸的方法 |
CN110396493B (zh) * | 2019-09-09 | 2021-09-07 | 廊坊梅花生物技术开发有限公司 | 培养基组合及生产异亮氨酸的方法 |
CN114606275A (zh) * | 2022-02-11 | 2022-06-10 | 安徽丰原发酵技术工程研究有限公司 | 一种发酵生产l-异亮氨酸的方法 |
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Inventor after: Guan Hui Inventor after: Zhong Yingdong Inventor after: Ye Peng Inventor after: Gong Yingzhang Inventor after: Cheng Zhaoquan Inventor after: Chen Zhiying Inventor before: Zhong Yingdong Inventor before: Ye Peng Inventor before: Gong Yingzhang Inventor before: Cheng Zhaoquan Inventor before: Chen Zhiying |
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Denomination of invention: A method of producing L-isoleucine by fermentation Effective date of registration: 20210408 Granted publication date: 20190910 Pledgee: Bank of China Limited by Share Ltd. Hohhot Xinhua Branch Pledgor: INNER MONGOLIA BIOK BIOLOGY Co.,Ltd. Registration number: Y2021150000031 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
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Granted publication date: 20190910 Pledgee: Bank of China Limited by Share Ltd. Hohhot Xinhua Branch Pledgor: INNER MONGOLIA BIOK BIOLOGY Co.,Ltd. Registration number: Y2021150000031 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A fermentation method for producing L-isoleucine Granted publication date: 20190910 Pledgee: Bank of China Limited by Share Ltd. Hohhot Xinhua Branch Pledgor: INNER MONGOLIA BIOK BIOLOGY Co.,Ltd. Registration number: Y2024150000059 |