CN109601776A - A kind of feed and preparation method thereof suitable for grass carp cultivation - Google Patents
A kind of feed and preparation method thereof suitable for grass carp cultivation Download PDFInfo
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- CN109601776A CN109601776A CN201910072839.9A CN201910072839A CN109601776A CN 109601776 A CN109601776 A CN 109601776A CN 201910072839 A CN201910072839 A CN 201910072839A CN 109601776 A CN109601776 A CN 109601776A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a kind of feed and preparation method thereof suitable for grass carp cultivation, belongs to field of feed.Feed of the present invention is prepared by the raw material of following parts by weight: 2-5 parts of 10-20 parts of powder of pork, 10-20 portions of corn flour, 10-20 portions of soybean residues, 10-20 parts of soybean powders, 10-20 portions of fishbone dusts, 10-20 parts of broad bean powders, 5-10 parts of rapeseed meal, 0.1-0.3 parts of Chinese herbal medicine powder, 3-5 parts of microorganism formulation and polylactic acid/hydroxy acetic acid nanosphere.The present invention by addition nanosphere so that Chinese herbal medicine powder efficacy stability and it is lasting, greatly reduce the usage amount of Chinese herbal medicine, while cooperating the use of microorganism formulation, grass carp autoimmunity can be promoted, while promoting yield, improve grass carp meat level.The formula of the invention is reasonable, and dosage is accurate, the destruction of high degree reduced to water nutrition environment, and preparation process is simple, easy to operate, significant effect.
Description
Technical field
The invention belongs to field of feed, and in particular to a kind of feed and preparation method thereof suitable for grass carp cultivation.
Background technique
Grass carp category Cypriniformes Cyprinidae Leuciscinae grass carp category.Grass carp has been commonly called as: grass carp, grass carp, oily grass carp, careless grass carp, ctenopharyngodon idellus,
Grass carp, grass roots (northeast), thick sub- fish (southern Shandong), sea otter (south), charlatan, black black carp etc..Grass carp inhabits the river of plains region
River and lake pool, general happiness occupy middle lower layer and the more water plant regions of offshore of water.Property it is active, swimming rapidly, often hunt in packs.For typical case
Herbivorous fishes.The young grass carps phase then eats larva, algae etc., and grass carp also eats of animal food, such as earthworm, dragonfly etc..In mainstream
Or it is overwintering at the deep water in lake.Breeding season parent population, which is traced back, swims habit.The many countries for growing Asia, Europe, the United States, non-each continent are moved.
Rapidly because of its growth, feed resource is wide for grass carp, is one of four large Chinese carps of CHINESE FRESHWATER cultivation.
With flourishing for culture fishery, the expansion of cultured area and increasing for type are only remote by natural bait
Far from meeting the needs of aquaculture production.Therefore, it is necessary to according to the different feeding habits and nutrition of various fish and aquatic livestock
It is required that making efficient artifical compound feed carries out feeding for science, can just increase economic efficiency.
The shortcomings that existing feed of grass carp is: one, feed is kept the growth cycle of grass carp long, is increased based on dark green grass
Aquaculture cost；Two, feed nutrient is single, cannot fully meet the growth demand of grass carp, will affect the yield and grass of grass carp
The nutritive value of meat quality of fish.
With the improvement of the quality of life, requirement of the people to grass carp meat is higher and higher, therefore different measure is taken to improve
Grass carp meat quality becomes current research hotspot.
Summary of the invention
The phenomenon that for the decline of current grass carp meat level, the invention is intended to provide a kind of nutrition-balanced grass carp cultivation feeding
Material promotes Growth of Grass Carps Ctenopharyngodon Idellus, while grass carp yield is substantially improved, improves grass carp meat level, promotes mouthfeel and trophic level.
The purpose to realize the present invention, used technical solution is:
A kind of feed suitable for grass carp cultivation, is prepared by the raw material of following parts by weight: 10-20 parts of powder of pork,
10-20 portions of corn flour, 10-20 portions of soybean residues, 10-20 parts of soybean powders, 10-20 portions of fishbone dusts, 10-20 parts of broad bean powders, rapeseed meal 5-
2-5 parts of 10 parts, 0.1-0.3 parts of Chinese herbal medicine powder, 3-5 parts of microorganism formulation and polylactic acid/hydroxy acetic acid nanosphere；
The polylactic acid/hydroxy acetic acid nanosphere is as made from following methods: taking 25mg polylactic acid/hydroxy acetic acid molten
In methylene chloride, 100 μ L liquid perflexanes are added under condition of ice bath, ultrasonic 5min adds the poly- of mixture quality 3%
Vinyl alcohol, homogeneous dispersion machine homogeneous 5min obtain emulsion；Emulsion, which is sufficiently stirred, makes methylene chloride volatilize, through distillation water washing,
Polylactic acid/hydroxy acetic acid nanosphere is obtained after centrifugation.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:3-5, and drying and grinding Cheng Fenhou is obtained.
The microorganism formulation be by bacillus subtilis, antibacterial peptide and enterococcus faecalis according to mass ratio 1:10:1 mixing and
At.
A kind of preparation method of the feed suitable for grass carp cultivation, comprising the following steps:
(1) 25mg polylactic acid/hydroxy acetic acid is taken to be dissolved in methylene chloride, be added under condition of ice bath 100 μ L liquid perfluors oneself
Alkane, ultrasonic 5min add the polyvinyl alcohol of mixture quality 3%, and homogeneous dispersion machine homogeneous 5min obtains emulsion；Emulsion is filled
Divide stirring that methylene chloride is made to volatilize, obtains polylactic acid/hydroxy acetic acid nanosphere after distillation water washing, centrifugation；
(2) Chinese herbal medicine powder is uniformly mixed with nanosphere obtained by step (1), obtains mixing A；
(3) according to the ratio by powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal after mixing
It crushes, microorganism formulation is added, is uniformly mixing to obtain mixed material B；
(4) mixed material B obtained by mixing A obtained by step (2) and step (3) is subjected to extruding granulation after mixing, has been granulated
Finished product packing is carried out after being dried, cooled down after, being sieved to get feed of the present invention.
Preferably, step (3) powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are uniformly mixed
Smash it through 100 meshes.
Preferably, step (4) drying temperature is 60 DEG C, drying time 1h.
Bacillus subtilis, antibacterial peptide used in the present invention and enterococcus faecalis are commercially available gained.Bacillus subtilis is effective
Content 2 × 1010cfu/g。
Bacillus subtilis and enterococcus faecalis (one kind of lactic acid bacteria), itself contains a large number of nutrients first, can be with
The effect of nutritional supplementation is played to organism；Secondly metabolic process can produce digestion and suction of a variety of enzymatic precession objects to food
It receives, and adherency of the inhibition pathogen of contestable to enteron aisle；Its last metabolic process generate organic acid, bacteriocin etc. inhibit or
Kill harmful bacteria.
And antibacterial peptide is active peptides substance, it can be (bacterium, plant, mollusk, amphibious dynamic by a variety of organisms
Object, fish, birds and mammal) it is generated through induction, have the effects that broad-spectrum sterilization, anti-viral, suppression tumor killing cell, is raw
The important component of object body nospecific immunity is added in aquatic animal feed as feed addictive in production,
Not only noresidue, it is pollution-free, immune have to the growth of animal and certain facilitation.
Cortex Eucommiae category Eucommiaceae Cortex Eucommiae category has very high economic value and medical value.Cortex Eucommiae can improve pig growth performance
With meat, increase chicken, hybridized prussian carp, grass carp collagen of muscle content, the immune function of chicken, hybridized prussian carp is improved.
Honeysuckle is also known as honeysuckle flowers, honeysuckle, for Caprifoliaceae and is that perennial evergreen winds liana, honeysuckle is made
For a kind of Chinese herb feed additive, by improving resistance bacterium infection, the immunity such as disease-resistant of fish body itself, especially to fish
The generation for preventing and reducing the Animal diseases such as aquatic products in terms of the aquatic livestocks body such as class nospecific immunity, is applied to livestock and poultry water
In the feed for producing animal, to promote the quality and yield of fish.However excessive use Chinese herbal medicine class additive, it can be to water
Body has an adverse effect, and influences the ecological balance of water environment.Therefore using Chinese herb feed additive it is critical that its play most
While big beneficial effect, its additive amount is reduced to the greatest extent.
Therefore the beneficial effects of the present invention are: the present invention is made after being sufficiently mixed with Chinese herbal medicine powder by addition nanosphere
Grain prepares feed of grass carp, the addition of nanoparticle, so that Chinese herbal medicine powder can be fully dispersed after entering in grass carp body, while right
The effect of Chinese herbal medicine powder is adequately adsorbed, and sustained release is played so that efficacy stability and it is lasting, greatly reduce adding for Chinese herbal medicine
Dosage reduces its pollution to water body.Cooperate the use of microorganism formulation simultaneously, microorganism formulation of the present invention is by withered grass gemma
Bacillus, enterococcus faecalis and antibacterial peptide mix according to a certain percentage, while cooperating Chinese herbal medicine to play drug effect, can promote grass
Fish autoimmunity improves grass carp meat level, promotes quality while promoting yield.The formula of the invention is reasonable, dosage essence
Standard, while guaranteeing to promote grass carp yield and quality, the destruction of high degree reduced to water nutrition environment, and prepare
Simple process, easy to operate, significant effect are suitble to promote and apply.
Specific embodiment
Technical scheme is described further combined with specific embodiments below, but not limited to this.
Embodiment 1
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 10 parts of powder of pork, 10 parts
Corn flour, 10 portions of soybean residues, 10 parts of soybean powders, 10 portions of fishbone dusts, 10 parts of broad bean powders, 5 parts of rapeseed meal, 0.1 part of Chinese herbal medicine powder, micro- life
2 parts of 3 parts of object preparation and polylactic acid/hydroxy acetic acid nanosphere；
The polylactic acid/hydroxy acetic acid nanosphere is as made from following methods: taking 25mg polylactic acid/hydroxy acetic acid molten
In methylene chloride, 100 μ L liquid perflexanes are added under condition of ice bath, ultrasonic 5min adds the poly- of mixture quality 3%
Vinyl alcohol, homogeneous dispersion machine homogeneous 5min obtain emulsion；Emulsion, which is sufficiently stirred, makes methylene chloride volatilize, through distillation water washing,
Polylactic acid/hydroxy acetic acid nanosphere is obtained after centrifugation.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:3, and drying and grinding Cheng Fenhou is obtained.
The microorganism formulation be by bacillus subtilis, antibacterial peptide and enterococcus faecalis according to mass ratio 1:10:1 mixing and
At.
A kind of preparation method of the feed suitable for grass carp cultivation, comprising the following steps:
(1) 25mg polylactic acid/hydroxy acetic acid is taken to be dissolved in methylene chloride, be added under condition of ice bath 100 μ L liquid perfluors oneself
Alkane, ultrasonic 5min add the polyvinyl alcohol of mixture quality 3%, and homogeneous dispersion machine homogeneous 5min obtains emulsion；Emulsion is filled
Divide stirring that methylene chloride is made to volatilize, obtains polylactic acid/hydroxy acetic acid nanosphere after distillation water washing, centrifugation；
(2) Chinese herbal medicine powder is uniformly mixed with nanosphere obtained by step (1), obtains mixing A；
(3) according to the ratio by powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal after mixing
It crushes, microorganism formulation is added, is uniformly mixing to obtain mixed material B；
(4) mixed material B obtained by mixing A obtained by step (2) and step (3) is subjected to extruding granulation after mixing, has been granulated
Finished product packing is carried out after being dried, cooled down after, being sieved to get feed of the present invention.
After step (3) powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are uniformly mixed crushing
It sieves with 100 mesh sieve.
Step (4) drying temperature is 60 DEG C, drying time 1h.
Bacillus subtilis, antibacterial peptide and enterococcus faecalis used are commercially available gained.Bacillus subtilis effective content 2 ×
1010cfu/g。
Embodiment 2
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 15 parts of powder of pork, 15 parts
Corn flour, 15 portions of soybean residues, 15 parts of soybean powders, 15 portions of fishbone dusts, 15 parts of broad bean powders, 8 parts of rapeseed meal, 0.2 part of Chinese herbal medicine powder, micro- life
3 parts of 4 parts of object preparation and polylactic acid/hydroxy acetic acid nanosphere；
The polylactic acid/hydroxy acetic acid nanosphere is as made from following methods: taking 25mg polylactic acid/hydroxy acetic acid molten
In methylene chloride, 100 μ L liquid perflexanes are added under condition of ice bath, ultrasonic 5min adds the poly- of mixture quality 3%
Vinyl alcohol, homogeneous dispersion machine homogeneous 5min obtain emulsion；Emulsion, which is sufficiently stirred, makes methylene chloride volatilize, through distillation water washing,
Polylactic acid/hydroxy acetic acid nanosphere is obtained after centrifugation.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:4, and drying and grinding Cheng Fenhou is obtained.
The microorganism formulation be by bacillus subtilis, antibacterial peptide and enterococcus faecalis according to mass ratio 1:10:1 mixing and
At.
A kind of preparation method of the feed suitable for grass carp cultivation, comprising the following steps:
(1) 25mg polylactic acid/hydroxy acetic acid is taken to be dissolved in methylene chloride, be added under condition of ice bath 100 μ L liquid perfluors oneself
Alkane, ultrasonic 5min add the polyvinyl alcohol of mixture quality 3%, and homogeneous dispersion machine homogeneous 5min obtains emulsion；Emulsion is filled
Divide stirring that methylene chloride is made to volatilize, obtains polylactic acid/hydroxy acetic acid nanosphere after distillation water washing, centrifugation；
(2) Chinese herbal medicine powder is uniformly mixed with nanosphere obtained by step (1), obtains mixing A；
(3) according to the ratio by powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal after mixing
It crushes, microorganism formulation is added, is uniformly mixing to obtain mixed material B；
(4) mixed material B obtained by mixing A obtained by step (2) and step (3) is subjected to extruding granulation after mixing, has been granulated
Finished product packing is carried out after being dried, cooled down after, being sieved to get feed of the present invention.
After step (3) powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are uniformly mixed crushing
It sieves with 100 mesh sieve.
Step (4) drying temperature is 60 DEG C, drying time 1h.
Bacillus subtilis, antibacterial peptide used in the present invention and enterococcus faecalis are commercially available gained.Bacillus subtilis is effective
Content 2 × 1010cfu/g。
Embodiment 3
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 20 parts of powder of pork, 20 parts
It is corn flour, 20 portions of soybean residues, 20 parts of soybean powders, 20 portions of fishbone dusts, 20 parts of broad bean powders, 10 parts of rapeseed meal, 0.3 part of Chinese herbal medicine powder, micro-
5 parts of 5 parts of biological agent and polylactic acid/hydroxy acetic acid nanosphere；
The polylactic acid/hydroxy acetic acid nanosphere is as made from following methods: taking 25mg polylactic acid/hydroxy acetic acid molten
In methylene chloride, 100 μ L liquid perflexanes are added under condition of ice bath, ultrasonic 5min adds the poly- of mixture quality 3%
Vinyl alcohol, homogeneous dispersion machine homogeneous 5min obtain emulsion；Emulsion, which is sufficiently stirred, makes methylene chloride volatilize, through distillation water washing,
Polylactic acid/hydroxy acetic acid nanosphere is obtained after centrifugation.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:5, and drying and grinding Cheng Fenhou is obtained.
The microorganism formulation be by bacillus subtilis, antibacterial peptide and enterococcus faecalis according to mass ratio 1:10:1 mixing and
At.
A kind of preparation method of the feed suitable for grass carp cultivation, comprising the following steps:
(1) 25mg polylactic acid/hydroxy acetic acid is taken to be dissolved in methylene chloride, be added under condition of ice bath 100 μ L liquid perfluors oneself
Alkane, ultrasonic 5min add the polyvinyl alcohol of mixture quality 3%, and homogeneous dispersion machine homogeneous 5min obtains emulsion；Emulsion is filled
Divide stirring that methylene chloride is made to volatilize, obtains polylactic acid/hydroxy acetic acid nanosphere after distillation water washing, centrifugation；
(2) Chinese herbal medicine powder is uniformly mixed with nanosphere obtained by step (1), obtains mixing A；
(3) according to the ratio by powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal after mixing
It crushes, microorganism formulation is added, is uniformly mixing to obtain mixed material B；
(4) mixed material B obtained by mixing A obtained by step (2) and step (3) is subjected to extruding granulation after mixing, has been granulated
Finished product packing is carried out after being dried, cooled down after, being sieved to get feed of the present invention.
After step (3) powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are uniformly mixed crushing
It sieves with 100 mesh sieve.
Step (4) drying temperature is 60 DEG C, drying time 1h.
Bacillus subtilis, antibacterial peptide used in the present invention and enterococcus faecalis are commercially available gained.Bacillus subtilis is effective
Content 2 × 1010cfu/g。
Comparative example 1
The preparation of the feed of the grass carp cultivation of no polylactic acid/hydroxy acetic acid nanosphere:
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 20 parts of powder of pork, 20 parts
Corn flour, 20 portions of soybean residues, 20 parts of soybean powders, 20 portions of fishbone dusts, 20 parts of broad bean powders, 10 parts of rapeseed meal, 0.3 part of Chinese herbal medicine powder and micro-
5 parts of biological agent.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:5, and drying and grinding Cheng Fenhou is obtained.
Preparation method with embodiment 3, unlike without polylactic acid/hydroxy acetic acid nanosphere preparation and added
Journey.
Comparative example 2
The preparation of the feed of the grass carp cultivation of no Chinese herbal medicine powder:
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 20 parts of powder of pork, 20 parts
Corn flour, 20 portions of soybean residues, 20 parts of soybean powders, 20 portions of fishbone dusts, 20 parts of broad bean powders, 10 parts of rapeseed meal, 5 parts of microorganism formulation and poly-
Lactic acid/5 parts of hydroxyacetic acid nanosphere.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:5, and drying and grinding Cheng Fenhou is obtained.
Preparation method is with embodiment 3, the difference is that the not preparation and adding procedure of Chinese herbal medicine powder.
Comparative example 3
The preparation of the feed of the grass carp cultivation of no microorganism formulation:
A kind of feed suitable for grass carp cultivation, is to be prepared by the raw material of following parts by weight: 20 parts of powder of pork, 20 parts
Corn flour, 20 portions of soybean residues, 20 parts of soybean powders, 20 portions of fishbone dusts, 20 parts of broad bean powders, 10 parts of rapeseed meal, 0.3 part of Chinese herbal medicine powder and poly-
Lactic acid/5 parts of hydroxyacetic acid nanosphere.
The Chinese herbal medicine powder is to be mixed by Cortex Eucommiae and honeysuckle according to mass ratio 1:5, and drying and grinding Cheng Fenhou is obtained.
Preparation method is with embodiment 3, the difference is that the not adding procedure of microorganism formulation.
Experimental example
Experiment sets 7 processing groups, embodiment 1-3 feed group, comparative example 1-3 and common commercially available feed of grass carp group altogether.
Test is purchased from Linyi City aquatic farm with grass carp, and it is (initial to choose the individual that 140 tail specifications are consistent, physical strong
150.2 ± 0.5g of weight), 7 groups are randomly divided into, are randomly assigned in 7 net cages (1.5 × 1.2 × 1.2), net cage is disposed within
In cement pit (5.0 × 3.0 × 1.2), every 20 tail fish of net cage, feeding time is 12 weeks.Daily by weight 3-5% divide 3 times (7:
30,12:00,16:30) it feeds, divide two-wheeled to feed every time, every wheel Feeding time about 20 minutes, each net cage keeps almost the same
Feeding amount, and situations such as ingesting and increase weight according to weather, water temperature and fish body is adjusted accordingly, to feed after bottom of pond without residue
Feed is advisable.During cultivation, every 7 days soil pick-ups are primary, change within every 3-4 days that water is primary, and water body is uninterruptedly inflated for 24 hours, dissolved oxygen > 5.0mg/
L, pH7.1-7.8,25-32 DEG C of water temperature, ammonia nitrogen concentration < 0.2mg/L, nitrite < 0.1mg/L.
Cultivation terminates, and stops eating for 24 hours, counts every net cage grass carp mantissa and claims gross weight, calculates rate of body weight gain；Every net cage randomly selects
5 tail fishes (every processing group totally 20 tail).It takes muscle of back 25g to be placed in -20 DEG C of freezings, is analyzed for muscle conventional ingredient；Take grass carp
Right side muscle of back sample (0.5 × 0.5 × 2cm) is stored in fixer for histomorphometric analysis；Separately take 3 pieces respectively about 3 grams
The muscle of back of weight, is immediately available for the measurement of muscle percentage of water loss (centrifugal process).
Centrifugal process: taking about 3g dorsal muscles, weighing, is put into 5mL centrifugation bottom of the tube, is placed in a centrifuge 3000g centrifugation 10min,
It wipes surface moisture away, weighs end weight, centrifugation the ratio between bodies lost weight and initial weight are to be centrifuged percentage of water loss.
Diameter of muscle fiber density measurement: by muscle samples, fixation is taken out afterwards for 24 hours in BouinShi liquid, respectively through 70%,
85%, 90% and 100% dehydrated alcohol serial dehydration, be placed in it is transparent in dimethylbenzene, waxdip and embedding, slice (6 μm), exhibition piece with
It dries piece, with mounting after hematoxylin eosin staining, is used for morphological analysis.Muscle fibre using image analysis system observation muscle is special
Sign, 300 muscle fibres of each sample number measure diameter of muscle fiber (20 times of mirrors).
In addition, flavor subjective appreciation is carried out after same cooking method culinary art system is ripe for the grass carp that each group is fed,
It invites 10 subjective appreciation personnel to carry out sensory evaluation to above-mentioned 7 groups of grass carps, makes comprehensive conclusion.
The experimental results are shown inthe following table for table 1:
As can be seen from the table, feed obtained by the embodiment of the present invention can effectively improve the meat level of grass carp,
Quality and yield are promoted, reduces disease incidence, all indicators are better than comparative examples and normal diet control group.And nanoparticle is lacked
The feed of the comparative example 1-3 of son, Chinese herbal medicine powder and microorganism formulation, the survival rate and nutritive index of grass carp are compared with embodiment
Difference, feeding effect are on close level with normal diet group.It may be speculated that nanoparticle, Chinese herbal medicine powder and micro- life that the present invention adds
Object preparation, interaction, has water body certain bactericidal and bacteriostatic effect, at the same improve to a certain degree grass carp from
Body immunity, to maintain higher survival rate.Meanwhile each ingredient of the present invention, have certain attractant effect for grass carp,
So that grass carp weight gain is significant, the scientific matching of nutriment of the present invention, so that the grass carp fed is Fresh & Tender in Texture, excellent taste,
And preparation process is simple, it is easy to spread.
It should be noted that above-described embodiment is only the section Example for realizing preferred embodiment of the invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments obtained, should fall within the scope of the present invention.
Claims (6)
1. a kind of feed suitable for grass carp cultivation, which is characterized in that be to be prepared by the raw material of following parts by weight: 10-20
Part powder of pork, 10-20 portions of corn flour, 10-20 portions of soybean residues, 10-20 parts of soybean powders, 10-20 portions of fishbone dusts, 10-20 parts of semen viciae fabaes
Powder, 5-10 parts of rapeseed meal, 0.1-0.3 parts of Chinese herbal medicine powder, 3-5 parts of microorganism formulation and polylactic acid/hydroxy acetic acid nanosphere 2-5
Part；
The polylactic acid/hydroxy acetic acid nanosphere is as made from following methods: 25mg polylactic acid/hydroxy acetic acid being taken to be dissolved in two
In chloromethanes, 100 μ L liquid perflexanes are added under condition of ice bath, ultrasonic 5min adds the polyethylene of mixture quality 3%
Alcohol, homogeneous dispersion machine homogeneous 5min obtain emulsion；Emulsion, which is sufficiently stirred, makes methylene chloride volatilize, through distillation water washing, centrifugation
Polylactic acid/hydroxy acetic acid nanosphere is obtained afterwards.
2. being suitable for the feed of grass carp cultivation according to claim 1, which is characterized in that the Chinese herbal medicine powder be by Cortex Eucommiae and
Honeysuckle mixes according to mass ratio 1:3-5, and drying and grinding Cheng Fenhou is obtained.
3. being suitable for the feed of grass carp cultivation according to claim 1, which is characterized in that the microorganism formulation is by withered grass
Bacillus, antibacterial peptide and enterococcus faecalis are mixed according to mass ratio 1:10:1.
4. being suitable for the preparation method of the feed of grass carp cultivation according to claim 1 to 3, which is characterized in that packet
Include following steps:
(1) it takes 25mg polylactic acid/hydroxy acetic acid to be dissolved in methylene chloride, 100 μ L liquid perflexanes is added under condition of ice bath, surpass
Sound 5min, adds the polyvinyl alcohol of mixture quality 3%, and homogeneous dispersion machine homogeneous 5min obtains emulsion；Emulsion is sufficiently stirred
Mixing makes methylene chloride volatilize, and obtains polylactic acid/hydroxy acetic acid nanosphere after distillation water washing, centrifugation；
(2) Chinese herbal medicine powder is uniformly mixed with nanosphere obtained by step (1), obtains mixing A；
(3) powder of pork, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are crushed after mixing according to the ratio,
Microorganism formulation is added, is uniformly mixing to obtain mixed material B；
(4) mixed material B obtained by mixing A obtained by step (2) and step (3) is subjected to extruding granulation after mixing, after the completion of granulation
Finished product packing is carried out after being dried, cooled down, being sieved to get feed of the present invention.
5. being suitable for the preparation method of the feed of grass carp cultivation according to claim 4, which is characterized in that step (3) pork
Powder, corn flour, soybean residue, soybean powder, fishbone dust, broad bean powder and rapeseed meal are uniformly mixed and smash it through 100 meshes.
6. being suitable for the preparation method of the feed of grass carp cultivation according to claim 4, which is characterized in that step (4) drying
Temperature is 60 DEG C, drying time 1h.
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| CN201910072839.9A CN109601776A (en) | 2019-01-25 | 2019-01-25 | A kind of feed and preparation method thereof suitable for grass carp cultivation |
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| CN201910072839.9A CN109601776A (en) | 2019-01-25 | 2019-01-25 | A kind of feed and preparation method thereof suitable for grass carp cultivation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112471011A (en) * | 2020-12-08 | 2021-03-12 | 通威股份有限公司 | Three-dimensional aquaculture method suitable for saline-alkali soil area |
| CN114557401A (en) * | 2022-03-15 | 2022-05-31 | 深圳桦晟生物科技有限公司 | Compound microbial feed and preparation method thereof |
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| CN101941675A (en) * | 2009-07-05 | 2011-01-12 | 王旭 | Nanoparticles and preparation method thereof |
| CN103652475A (en) * | 2013-12-07 | 2014-03-26 | 济南凯因生物科技有限公司 | Feed for grass carp |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941675A (en) * | 2009-07-05 | 2011-01-12 | 王旭 | Nanoparticles and preparation method thereof |
| CN103652475A (en) * | 2013-12-07 | 2014-03-26 | 济南凯因生物科技有限公司 | Feed for grass carp |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112471011A (en) * | 2020-12-08 | 2021-03-12 | 通威股份有限公司 | Three-dimensional aquaculture method suitable for saline-alkali soil area |
| CN114557401A (en) * | 2022-03-15 | 2022-05-31 | 深圳桦晟生物科技有限公司 | Compound microbial feed and preparation method thereof |
| CN114557401B (en) * | 2022-03-15 | 2024-04-19 | 云南大台农台标农业科技有限公司 | Composite microbial feed and preparation method thereof |
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