CN109393204A - A kind of compound micro-ecological preparation and its application for the cultivation of penaeus penicillatus mark seedling - Google Patents
A kind of compound micro-ecological preparation and its application for the cultivation of penaeus penicillatus mark seedling Download PDFInfo
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- CN109393204A CN109393204A CN201811333185.2A CN201811333185A CN109393204A CN 109393204 A CN109393204 A CN 109393204A CN 201811333185 A CN201811333185 A CN 201811333185A CN 109393204 A CN109393204 A CN 109393204A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 150000001875 compounds Chemical class 0.000 title claims abstract description 39
- 241000335026 Fenneropenaeus penicillatus Species 0.000 title claims abstract description 28
- 241000238557 Decapoda Species 0.000 claims abstract description 94
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 76
- 244000005700 microbiome Species 0.000 claims abstract description 55
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 239000006041 probiotic Substances 0.000 claims abstract description 17
- 235000018291 probiotics Nutrition 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 13
- 238000009360 aquaculture Methods 0.000 claims abstract description 11
- 244000144974 aquaculture Species 0.000 claims abstract description 11
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 8
- 235000012054 meals Nutrition 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims description 23
- 230000000813 microbial effect Effects 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 11
- 230000009286 beneficial effect Effects 0.000 claims description 10
- 238000010790 dilution Methods 0.000 claims description 10
- 239000012895 dilution Substances 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 8
- 244000060924 Brassica campestris Species 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 230000035772 mutation Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 2
- 239000003124 biologic agent Substances 0.000 claims 1
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 206010047400 Vibrio infections Diseases 0.000 abstract description 8
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 4
- 241000927735 Penaeus Species 0.000 abstract description 3
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- 238000012360 testing method Methods 0.000 description 13
- 238000009395 breeding Methods 0.000 description 12
- 230000001488 breeding effect Effects 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 230000000855 fungicidal effect Effects 0.000 description 10
- 239000000417 fungicide Substances 0.000 description 10
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- 230000008901 benefit Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 239000004568 cement Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000013535 sea water Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000366 juvenile effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
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- 230000029087 digestion Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
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- 238000007254 oxidation reaction Methods 0.000 description 2
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- 239000007787 solid Substances 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 208000031872 Body Remains Diseases 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 239000000877 Sex Attractant Substances 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- VGPSUIRIPDYGFV-UHFFFAOYSA-N [N].O[N+]([O-])=O Chemical compound [N].O[N+]([O-])=O VGPSUIRIPDYGFV-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004176 ammonification Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
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- 238000012372 quality testing Methods 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
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- 238000011105 stabilization Methods 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Immunology (AREA)
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- Environmental Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Botany (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
Compound micro-ecological preparation and its application the invention discloses one of probiotics technical field for the cultivation of penaeus penicillatus mark seedling, the compound micro-ecological preparation, including below according to the raw material of mass percent meter: compound microorganism bacterium powder 15~30%, oligoisomaltose 1.0~5.0%, coarse colza meal 65~80%, edible essence 0.1~0.2%.The application of probiotics of the invention in prawn culturing, application method can promote penaeus penicillatus growth, improve immunity of prawn and premunition, reduce shrimp disease, especially for the prevention and treatment of vibriosis penaeus evil, purifying aquaculture water quality, probiotics can be applied in preparing veterinary drug, feed addictive or water quality cleansing agent etc..
Description
Technical field
The present invention relates to probiotics technical field, specifically a kind of composite microbial for the cultivation of penaeus penicillatus mark seedling
State preparation and its application.
Background technique
Aquaculture is the important component of agricultural avocation production, mostly uses high-density breeding greatly at present.In aquaculture
During, feed, the excreta of aquatic animal and animals and plants corpse can constantly be accumulated in breeding water body, in anoxic
Under conditions of decomposed through the microbial fermentation in mud and generate ammonia nitrogen, sulfide, organic acid, a variety of nuisances such as nitrite
Matter causes water pollution serious.Meanwhile the water body environment of pollution breaks the ecosystem of original balance, it is floating in water body
Trip plant and water plant can not grow, and create advantage, water to the breeding and propagation of various harmful sex pheromones
The illness rate of production product improves, and seriously affects the economic benefit of culture fishery.
Prawn is the important economic kind of aquaculture, in China because it has higher economic benefit, so cultivation scale
It is rapidly developed with cultured area.In order to meet growing product demand, all kinds of chemicals were commonly used in the past and resisted
It is raw usually to inhibit pathogenic microorganism growth and breeding.However, a large amount of uses of antibiotic can cause autogenous infection, endurance strain
Generation, the problems such as remaining in aquatic products hypoimmunity and aquatic products and water body environment, therefore many countries and regions are all
It has prohibited and has used the antibiotic of growth-promoting class at feeding end.So being badly in need of wanting a kind of green and healthy method to push pair
The sustainable development of shrimp or even culture fishery.
For the problem more than the disease of prawn culturing low yield in recent years, effective complex microorganism preparations come into being.It is a large amount of real
Verify bright, microorganism formulation is one of antibiotic best substitute, has safe and reliable, nonhazardous effect, free from environmental pollution
The advantages that.Microorganism formulation it is compound as multiple-microorganism and cultivate made of, a large amount of carbohydrate, amino acid, vitamin can be synthesized
And physiological activator, it can promote fishes and shrimps growth；Contained a variety of digestive ferments, metabolic enzyme, ubiquinone and a large amount of carrotene etc.
The digestive utilization ratio that fishes and shrimps can be improved improves the immunity of fishes and shrimps, prevents the bacteriosis of fishes and shrimps, moreover it is possible to reduce harmful in water
Gas and ammonia-nitrogen content improve water quality.
Summary of the invention
The purpose of the present invention is to provide it is a kind of for penaeus penicillatus mark seedling cultivation compound micro-ecological preparation and its application,
To solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling, including below according to the original of mass percent meter
Material: compound microorganism bacterium powder 15~30%, oligoisomaltose 1.0~5.0%, coarse colza meal 65~80%, edible essence 0.1
~0.2%.
As a further solution of the present invention: including below according to the raw material of mass percent meter: compound microorganism bacterium powder
25%, oligoisomaltose 4.85%, coarse colza meal 70%, edible essence 0.15%.
As further scheme of the invention: the compound microorganism bacterium powder is saccharomycete, lactic acid bacteria, bacillus
Combination, mass ratio 1:1:1.
As further scheme of the invention: the probiotics are microbial cells fermentation liquid and remaining raw material
Mixture or microbial cells and remaining raw material mixture.
The application for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in prawn culturing.
A kind of application method for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in cultured prawn,
(1) 15 days before launching shrimp seedling, shrimp ponds are uniformly sprayed with 20-25kg by the composite bacteria liquid water that 5 ‰ hundred million An Qile ferment per acre
Shrimp ponds are spilt, purifying aquaculture environment is put before shrimp seedling 3 days, then with concentration is 10ml/m3Complex microorganism preparations dilution splash water
Face makes the beneficial microbe population in shrimp ponds form dominant population, is conducive to shrimp seedling pond-inputting and just adapts to grow；(2) shrimp is launched
Miao Hou, first time dosage are 10kg/667m2Complex microorganism preparations dilution add later every 10 days by every kilogram of feed
The amount for adding complex microorganism preparations is that 10-20g is fed at once after slightly stirring keeps its uniform；(3) water is encountered during feeding
There is floating in cytoplasmic mutation, shrimp, immediately with complex microorganism preparations (concentration 1kg/667m2) mix 2kg brown sugar
After water, full pool spilling head is carried out.
Compared with prior art, the beneficial effects of the present invention are: probiotics of the invention answering in prawn culturing
With application method can promote penaeus penicillatus growth, improves immunity of prawn and premunition, reduces shrimp disease, especially
Prevention and treatment for vibriosis penaeus evil, purifying aquaculture water quality, probiotics can be to prepare veterinary drug, feed addictive or water quality net
It is applied in agent etc..
Detailed description of the invention
Fig. 1 is that the quantity of vibrios in cement pit changes line chart.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment one:
A kind of compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling, including below according to the original of mass percent meter
Material: compound microorganism bacterium powder 30%, oligoisomaltose 4.8%, coarse colza meal 65%, edible essence 0.2%.
The compound microorganism bacterium powder is the combination of saccharomycete, lactic acid bacteria, bacillus, mass ratio 1:1:1.
The probiotics be microbial cells fermentation liquid and remaining raw material mixture or microbial cells with
The mixture of remaining raw material.
The application for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in prawn culturing.
A kind of application method for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in cultured prawn,
(1) 15 days before launching shrimp seedling, shrimp ponds uniformly spray shrimp by the composite bacteria liquid water that 5 ‰ hundred million An Qile ferment with 20kg per acre
Pond, purifying aquaculture environment are put before shrimp seedling 3 days, then with concentration are 10ml/m3Complex microorganism preparations dilution splash the water surface,
So that the beneficial microbe population in shrimp ponds is formed dominant population, is conducive to shrimp seedling pond-inputting and just adapts to grow；(2) shrimp seedling is launched
Afterwards, first time dosage is 10kg/667m2Complex microorganism preparations dilution, later every 10 days, by every kilogram of feed add
The amount of complex microorganism preparations is that 10g is fed at once after slightly stirring keeps its uniform；(3) it is prominent that water quality is encountered during feeding
Become, there is floating in shrimp, immediately with complex microorganism preparations (concentration 1kg/667m2) mix 2kg brown sugar water after,
Carry out full pool spilling head.
Embodiment two:
A kind of compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling, including below according to the original of mass percent meter
Material: compound microorganism bacterium powder 17%, oligoisomaltose 2.9%, coarse colza meal 80%, edible essence 0.1%.
The compound microorganism bacterium powder is the combination of saccharomycete, lactic acid bacteria, bacillus, mass ratio 1:1:1.
The probiotics be microbial cells fermentation liquid and remaining raw material mixture or microbial cells with
The mixture of remaining raw material.
The application for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in prawn culturing.
A kind of application method for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in cultured prawn,
It is characterized in that, (1) 15 days before launching shrimp seedling, shrimp ponds 25kg passes through the composite bacteria liquid water that 5 ‰ hundred million An Qile ferment per acre
Uniformly sprinkling shrimp ponds, purifying aquaculture environment puts before shrimp seedling 3 days, then with concentration is 10ml/m3Complex microorganism preparations dilution
It splashes the water surface, so that the beneficial microbe population in shrimp ponds is formed dominant population, be conducive to shrimp seedling pond-inputting and just adapt to grow；(2)
After launching shrimp seedling, first time dosage is 10kg/667m2Complex microorganism preparations dilution, later every 10 days, by every kilogram
The amount of feed addition complex microorganism preparations is that 20g is fed at once after slightly stirring keeps its uniform；(3) it is encountered during feeding
There is floating in water quality mutation, shrimp, immediately with complex microorganism preparations (concentration 1kg/667m2) mix 2kg brown sugar
After changing water, full pool spilling head is carried out.
Embodiment three:
A kind of compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling, including below according to the original of mass percent meter
Material: compound microorganism bacterium powder 25%, oligoisomaltose 4.85%, coarse colza meal 70%, edible essence 0.15%.
The compound microorganism bacterium powder is the combination of saccharomycete, lactic acid bacteria, bacillus, mass ratio 1:1:1.
The probiotics be microbial cells fermentation liquid and remaining raw material mixture or microbial cells with
The mixture of remaining raw material.
The application for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in prawn culturing.
A kind of application method for the compound micro-ecological preparation of penaeus penicillatus mark seedling cultivation in cultured prawn,
It is characterized in that, (1) 15 days before launching shrimp seedling, shrimp ponds 25kg passes through the composite bacteria liquid water that 5 ‰ hundred million An Qile ferment per acre
Uniformly sprinkling shrimp ponds, purifying aquaculture environment puts before shrimp seedling 3 days, then with concentration is 10ml/m3Complex microorganism preparations dilution
It splashes the water surface, so that the beneficial microbe population in shrimp ponds is formed dominant population, be conducive to shrimp seedling pond-inputting and just adapt to grow；(2)
After launching shrimp seedling, first time dosage is 10kg/667m2Complex microorganism preparations dilution, later every 10 days, by every kilogram
The amount of feed addition complex microorganism preparations is that 20g is fed at once after slightly stirring keeps its uniform；(3) it is encountered during feeding
There is floating in water quality mutation, shrimp, immediately with complex microorganism preparations (concentration 1kg/667m2) mix 2kg brown sugar
After changing water, full pool spilling head is carried out.
These microbial strains are preserved in Chinese industrial Microbiological Culture Collection administrative center (China Center of
Industrial Culture Collection, abbreviation CICC), mainly there are following five kinds of microorganisms: lactobacillus acidophilus
(Lactobacillus acidophilus, CICC 6074), German-style lactobacillus (Lactobacillus delbrueckii,
CICC 6256), Bacillus subtillis (Bacillus subtilis, CICC 20872), candida utili (Candida
Utilis, CICC 31188), saccharomyces cerevisiae (Saccharomyces cerevisiae, CICC 1854 and CICC 1480).
The existence form of the probiotics can be the mixture of microbial cells fermentation liquid and solid adjuvant material, or
The mixture of microbial cells and solid adjuvant material.
The application of probiotics of the invention in prawn culturing, the method can promote penaeus penicillatus growth, improve
Immunity of prawn and premunition, reduce shrimp disease, especially for the prevention and treatment of vibriosis penaeus evil, purifying aquaculture water
Matter.The probiotics can be applied in preparing veterinary drug, feed addictive or water quality cleansing agent etc..
Test one: the influence test of probiotics of the invention as feed addictive to the growth of penaeus penicillatus
Test is single factor experiment, and test site is located at Shandong Fresh Water Aquatic Product Research Institute, and the penaeus penicillatus of selection is purchased from
Xiamen Aquatic Production College's seawater seed rearing field.It is that 0.5 centimetre of 400 tail prawns are randomly divided into four groups by average external volume, every group 100
Tail cultivates respectively in cement pit in 1.2m × 1.0m × room 0.6m, carries out 33 days by a definite date raising (2018.5.23-
2018.6.24).Group 1 is control group: feeding the feed of no microorganism formulation feed addictive；Group 2, group 3, group 4 are experimental group,
The bait for feeding microorganism formulation feed addictive, the amount by every kilogram of feed addition complex microorganism preparations is 10g, 15g
And 20g, it is fed at once after slightly mixing thoroughly.Four groups are the same feed formulas, while bait of the feeding with dose daily
Expect, is similarly managed under same environment.Daily bait throwing in is secondary, morning 7:00 and 6:00 in afternoon, takes at random in breeding process
The measurement of 30 tail prawns.
The influence that 1 microorganism formulation of table grows prawn as feed addictive
As can be seen from the above table, with the progress of experiment, good line is presented in the body length and weight and culturing time of juvenile prawn
Sexual intercourse, being added to complex microorganism preparations group, (group 2, group 3 and the group speed of growth 4) and survival rate are obviously higher than control group
(group 1), wherein the long growth rate of body improves 60.7%, 64.6% and 69.9% respectively, and body weight growth rate improves respectively
206.3%, 231.2% and 275%, illustrate that the complex microorganism preparations being sprayed on feed have apparent growth promoting function, and
And the survival rate of prawn can be obviously improved.
In the cultivating process of prawn, vibrios (Vibrio spp.) endangers it larger.When environmental condition deterioration, prawn
When resistance reduces, vibrios will take advantage of a weak point, and cause the lesion of prawn, make its slow growth or even death.So we are also
The quantity of vibrios in cement pit is detected, as a result such as attached drawing 1.
The experiment later period has been arrived, (has organized 2, group 3 and the amount of vibrio organized in 4) are more right using the test group of complex micro organism fungicide
It is substantially reduced according to group (group 1), illustrates the activity after the beneficial microbe in complex micro organism fungicide enters water body obviously to vibrios
Stronger inhibiting effect is produced, the living environment of juvenile prawn is improved, also gives very low one of control group survival rate from side
A reason.
Test two: the influence of probiotics of the invention as water quality cleansing agent to water quality in prawn culturing water body is tested
Test is single factor experiment, and test site is located at Shandong Fresh Water Aquatic Product Research Institute, and the prawn of selection is purchased from Xiamen
Marine products institute's seawater seed rearing field.The 400 tail prawns that average external volume is 0.5 centimetre are randomly divided into four groups, every group of 100 tails divide
Raising (2018.5.23-2018.6.24) in 33 days by a definite date Yang Zhi not be carried out in cement pit in 1.2m × 1.0m × room 0.6m.Group
1 is control group: feeding the feed of no microorganism formulation feed addictive, breeding water body does not add complex micro organism fungicide；Group
2, group 3, group 4 are experimental group, feed the bait of microorganism formulation feed addictive, add complex microorganism by every kilogram of feed
The amount of preparation is 10g, 15g and 20g, is fed at once after slightly mixing thoroughly.Daily bait throwing in is secondary, morning 7:00 and 6:00 in afternoon.
In breeding process, daily using the temperature and dissolved oxygen (DO) of daily water quality testing meter measurement breeding water body, pH is used
The pH of meter measurement water body.Every 10 days water samplings, by miillpore filter vacuumizing filtration, filtrate measured for water quality indicator.Phase
It is as follows to close result:
The situation of change of dissolved oxygen amount (mg/L) in 3 prawn culturing water body of table
It can see from upper table, used test group (group 2, group 3 and the dissolved oxygen in water organized in 4) of complex micro organism fungicide
Amount is averagely higher by 1mg/L compared with control group (group 1), after the beneficial microbe being primarily due in complex micro organism fungicide enters water body
Flourish simultaneously forms a certain number of populations, decomposes the organic matter in water body and bed mud rapidly, and the group of being effectively reduced is organic
Object accumulate anaerobic digestion and caused by excessive DO consumption, plus its own oxygen demand is few, thus indirect increase water body
DO also ensures the normal growth of prawn.
The situation of change of pH value in 4 prawn culturing water body of table
PH value height in water directly affects the metabolism of prawn, serves to the growth of prawn vital.
It can see from upper table, the test group of complex micro organism fungicide used (to organize 2, the group 3 and pH organized in 4) is compared with control group (group 1)
Average low 1 pH out, the beneficial microbe being primarily due in complex micro organism fungicide enter after water body because wanting flourish,
Organic matter largely has been decomposed, so that pH is reduced, has facilitated the pH for controlling entire pond water quality in this way, reaches Cultivated water environment matter
The requirement of amount.
The situation of change of ammonia-nitrogen content (mg/L) in 5 prawn culturing water body of table
Ammonia nitrogen (NH4- N) it is the harmful substance generated in breeding process, excessive concentration can restrict the cultivation of prawn and cause
The environmental pollutions such as water eutrophication.The experiment later period has been arrived, (has organized 2, group 3 and group are 4) using the test group of complex micro organism fungicide
In ammonia-nitrogen content be substantially reduced compared with control group (group 1), illustrate that the beneficial microbe in complex micro organism fungicide can absorb and benefit
With the inorganic nitrogen in breeding water body, guarantee the health of prawn living environment.
The situation of change of cultured water content (mg/L) in 6 prawn culturing water body of table
Cultured water (NO in seawater2- N) it is used as nitrate nitrogen (NO3- N) and ammonium nitrogen (NH4- N) it restores and oxidation process
Intermediate product, content is relatively low and unstable, but content is relatively high in shrimp pond culture water environment, through frequently as a water
The performance assessment criteria of matter.
Cultured water (NO2- N) it is also the harmful substance generated in breeding process, excessive concentration can equally restrict prawn
Cultivation.The experiment later period has been arrived, the cultured water in control group (group 1) water persistently increases, reach 0.062mg/L, and use multiple
The test group for closing microbial bacterial agent (organizes 2, group 3 and the water body organized in 4) maintain lower level, this also indicates that the product of experiment
In, ammonification function class bacterium and nitrification function bacterium play in each change-session of biological deamination and act synergistically, harmful nitrous
Sour nitrogen is converted into harmless nitrate and mycoprotein.
The situation of change of nitric acid nitrogen content (mg/L) in 7 prawn culturing water body of table
Nitrate nitrogen (NO in seawater3- N) be itrogenous organic substance oxygenolysis final product.Nitrogen in water is with nitrate shape
State exists, and belongs to hypotoxicity or nontoxicity.Its changes of contents is mainly influenced by marine organisms activity and Organic oxidation decomposition.With
The increase of culturing time, the nitrate content in all groups have growth.But using the test group of complex micro organism fungicide
(for group 2, group 3 and the water body organized in 4) are compared with control group (group 1), the trend increased is more gentle, illustrates the heterotrophism in water environment
The nitrogen source in water is utilized in bacterium, forms microbe colony, and the inorganic nitrogen in absorption and assimilation water body remains related in water body
The stabilization of ecological factor.
In conclusion probiotics of the invention can enhance the premunition of prawn as feed addictive, promote prawn
Digestion and absorption growth, improve juvenile prawn survive and the problems such as at shrimp yield on have higher use value and catch after, no beast
The residual of medicine, hormone etc., can effectively replace antibiotic are particularly suitable for being widely applied in feed.As water purification agent, have in elimination
While machine object is polluted, is purified water, moreover it is possible to effectively inhibit harmful microbe growth and breeding in water body, have ideal
Application effect is it is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling, which is characterized in that including below according to quality hundred
Point than meter raw material: compound microorganism bacterium powder 15~30%, oligoisomaltose 1.0~5.0%, coarse colza meal 65~80%,
Edible essence 0.1~0.2%.
2. the compound micro-ecological preparation according to claim 1 for the cultivation of penaeus penicillatus mark seedling, which is characterized in that including
Below according to the raw material of mass percent meter: compound microorganism bacterium powder 25%, oligoisomaltose 4.85%, coarse colza meal
70%, edible essence 0.15%.
3. the compound micro-ecological preparation according to claim 1 for the cultivation of penaeus penicillatus mark seedling, which is characterized in that described
Compound microorganism bacterium powder is the combination of saccharomycete, lactic acid bacteria, bacillus, mass ratio 1:1:1.
4. the compound micro-ecological preparation according to claim 1 for the cultivation of penaeus penicillatus mark seedling, which is characterized in that described
Probiotics are the mixing of microbial cells fermentation liquid and the mixture or microbial cells and remaining raw material of remaining raw material
Object.
5. the compound micro-ecological preparation for the cultivation of penaeus penicillatus mark seedling as described in claim 1-4 is any is in prawn culturing
Application.
6. a kind of compound micro-ecological preparation as described in claim 1-4 for the cultivation of penaeus penicillatus mark seedling is in cultured prawn
Application method, which is characterized in that (1) 15 days before launching shrimp seedling, shrimp ponds pass through 5 ‰ hundred million An Qilefa with 20-25kg per acre
The composite bacteria liquid water of ferment uniformly sprays shrimp ponds, and purifying aquaculture environment is put before shrimp seedling 3 days, then with concentration is 10ml/m3It is compound micro-
Biological agent dilution is splashed the water surface, so that the beneficial microbe population in shrimp ponds is formed dominant population, is conducive to shrimp seedling pond-inputting just
It adapts to grow；(2) after launching shrimp seedling, first time dosage is 10kg/667m2Complex microorganism preparations dilution, often later
Every 10 days, the amount by every kilogram of feed addition complex microorganism preparations was that 10-20g is thrown at once after slightly stirring keeps its uniform
Hello；(3) encounter water quality mutation during feeding, there is floating in shrimp, be immediately 1kg/667m with concentration2It is compound
After microorganism formulation mixes 2kg brown sugar water, full pool spilling head is carried out.
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