CN115745195B - Water quality treating agent for aquaculture and application thereof - Google Patents
Water quality treating agent for aquaculture and application thereof Download PDFInfo
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- CN115745195B CN115745195B CN202211597074.9A CN202211597074A CN115745195B CN 115745195 B CN115745195 B CN 115745195B CN 202211597074 A CN202211597074 A CN 202211597074A CN 115745195 B CN115745195 B CN 115745195B
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Abstract
The invention provides a water quality treating agent for aquaculture and application thereof, belonging to the technical field of water quality treatment. The water quality treating agent for aquaculture provided by the invention comprises the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of compound nitrifying bacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides paraqualis. The water quality treating agent provided by the invention has the effects of improving water quality, increasing the oxygen content of water and reducing the death rate of sick fishes. The method is used in the field of aquaculture, and can remarkably improve the quality and yield of the aquatic products.
Description
Technical Field
The invention relates to the technical field of water quality treatment agents, in particular to a water quality treatment agent for aquaculture and application thereof.
Background
Along with the development of economy and society, the requirements of people on the quantity and quality of aquatic products are higher and higher, and in order to pursue high benefit, the stocking density is blindly increased, so that the aquaculture is developed to high density, intensification and industrialization, and the bait and antibiotics are added more and more due to the increase of the cultivation scale. The pollution of the water body is aggravated, and serious consequences such as hypoxia of the water body, killing of beneficial microorganisms, frequent occurrence of fish and shrimp diseases and the like are brought. Restricting the growth of fish and shrimp, resulting in reduced yield. Therefore, the water quality treating agent is used for sterilizing and disinfecting water quality, improving the transparency of water, reducing the ammonia nitrogen content of water, reducing the chemical oxygen consumption and the biochemical oxygen consumption and the like in the prior aquaculture process to improve the aquatic environment.
The biological water purifying agent is a kind of microecological preparation containing bacillus, enzyme and nutrient substances, and can be used for various links of intensive aquaculture. The substances such as filth, organic sediment, ammonia, nitrogen and the like in the culture pond can be degraded through the actions of microorganisms and enzymes, the organic waste is liquefied, and the water quality is purified. Microorganisms in the biological water purifying agent are used as important activity and transformation carriers, and play a role in water quality purification. The commonly used microbial agent is usually a composite microbial agent, for example, chinese patent CN101475265B provides a composite microbial agent, and the composite microbial agents I-III are coordinated and complemented to achieve the water purifying effect. However, the purified water body has different water quality conditions and pollution degrees due to different strain components and physical and chemical properties of the compound microbial preparation, so that the purification effect of the microbial preparation is greatly different.
Nutrient substances are the basis of the survival and propagation of the microbial inoculum, but different nutrient substances have different effects on the water purifying capacity of the strain. For example, commonly added nutrients include methanol, ethanol, acetic acid, sodium acetate, glucose, phosphorus, and the like. Research proves that the added glucose can improve the nitrification efficiency and purification capacity of microorganisms, and the research of scholars shows that the phosphorus deficiency can influence the purification effect of the water quality purification microbial inoculum.
Based on this, the present invention has been proposed to provide a water treatment agent for aquaculture.
Disclosure of Invention
The invention aims to provide a water quality treating agent for aquaculture, which is used for improving water quality, increasing oxygen content of water, reducing average death rate of aquatic products and improving yield.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a water quality treating agent for aquaculture, which comprises the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of compound nitrifying bacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides paraqualis.
Preferably, the amino acid culture medium comprises the following raw materials in parts by weight: 18 to 22 parts of peptone, 6 to 10 parts of yeast extract, 8 to 12 parts of brown sugar, 8 to 12 parts of urea, 3 to 7 parts of sodium chloride, 0.8 to 1.2 parts of tween 80, 0.5 to 0.7 part of glutamine, 0.5 to 0.7 part of histidine, 0.4 to 0.6 part of leucine, 0.4 to 0.6 part of methionine, 0.3 to 0.5 part of threonine, 0.3 to 0.5 part of valine, 0.2 to 0.4 part of aspartic acid, 0.1 to 0.3 part of glycine and 0.1 to 0.3 part of proline.
Preferably, the oligosaccharide is one or more of fructo-oligosaccharide, lactose oligosaccharide and isomaltose.
Preferably, the fructo-oligosaccharide is inulin-derived fructo-oligosaccharide or sucrose-derived fructo-oligosaccharide.
The invention also provides an application of the water quality treating agent in purifying aquaculture water quality.
The water quality treating agent provided by the invention has the effects of improving water quality, increasing the oxygen content of water and reducing the death rate of sick fishes. The water quality is clear and transparent after being used in the field of aquaculture, the oxygen content can be increased from 10.12mg/L to 24.38mg/L, the water belongs to high-quality water areas of aquaculture, and the water quality can be used for purifying the water quality of diseased fishes, so that the death rate of the diseased fishes can be greatly reduced, the quality and the yield of the aquatic products can be obviously improved, and the loss of farmers can be reduced.
Drawings
FIG. 1 is a graph showing the water quality improvement of the water quality treatment agent of example 1 before and after treatment;
FIG. 2 is a comparison of the results of two water treatment agents of example 2;
FIG. 3 is a comparison of the results of two water treatment agents of example 3;
FIG. 4 is a comparison of the results of the two water treatment agents of example 4.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The following raw materials were weighed and mixed to obtain an amino acid medium, 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 1 part of tween 80, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
43 parts of amino acid culture medium, 32 parts of sucrose-derived fructo-oligosaccharide, 11 parts of EM flora, 4 parts of compound nitrifying bacteria, 2 parts of yeast selenium and 0.3 part of bacteroides parapsilosis are weighed, and uniformly mixed and applied.
The water treatment agent prepared in example 1 was tested at an aquaculture base of jujube village, city, bottom pavilion in urban area. The application amount is 2 kg/mu once a week. One month later, the results are shown in FIG. 1. As can be seen from FIG. 1, the water quality before use is yellowish green, and has a large amount of moss. After one month of use, the water quality is clear and has no moss, and is suitable for aquaculture. The oxygen content of the water body before and after the use is measured to be 10.12mg/L before the use and 24.38mg/L after the use. Therefore, the water quality treating agent provided by the invention has the effects of improving water quality and increasing the oxygen content of water.
Comparative example 1
The difference between this comparative example and example 1 is that the medium used is a normal medium, and the composition ratio is as follows: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 1 part of tween 80 and 0.8 part of cysteine.
The formula of the water quality treating agent is as follows: 43 parts of common culture medium, 32 parts of sucrose-derived fructo-oligosaccharide, 11 parts of EM flora, 4 parts of compound nitrifying bacteria, 2 parts of yeast selenium and 0.3 part of bacteroides parahaemolyticus, and uniformly mixing and then applying.
The water treatment agents prepared in example 1 and comparative example 1 were used for the cultivation water treatment, respectively, at an application rate of 2 kg/mu once a week for 2 months, as shown in FIG. 2. As is apparent from fig. 2, the aquaculture water treated in example 1 is clear (right panel). The water treated in comparative example 1 was cloudy, yellow-green in color, and had little moss present (left panel).
Comparative example 2
This comparative example differs from example 1 in that glucose was used instead of fructo-oligosaccharides (sucrose source).
Amino acid medium: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 1 part of tween 80, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
The formula of the water quality treating agent comprises the following components: 43 parts of amino acid culture medium, 32 parts of glucose, 11 parts of EM flora, 4 parts of compound nitrifying bacteria, 2 parts of yeast selenium and 0.3 part of bacteroides paraqualis, and uniformly mixing and then applying.
The water treatment agents prepared in example 1 and comparative example 2 were used for water treatment in a rice-crab symbiotic system, respectively, at an application rate of 1.5 kg/mu once a week for 1 month, as shown in FIG. 3. As can be seen from FIG. 3, the rice-crab symbiotic system treated in example 1 was clear in water quality without moss impurities (left panel), while the rice-crab symbiotic system treated in comparative example 2 was crossed in water quality with a large amount of moss present (right panel).
Comparative example 3
This comparative example differs from example 1 in that the sucrose-derived fructooligosaccharides in example 1 are replaced by inulin-derived fructooligosaccharides.
Amino acid medium: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 1 part of tween 80, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
The formula of the water quality treating agent comprises the following components: 43 parts of amino acid culture medium, 32 parts of inulin-derived fructo-oligosaccharide, 11 parts of EM flora, 4 parts of compound nitrifying bacteria, 2 parts of yeast selenium and 0.3 part of bacteroides parahaemolyticus, and uniformly mixing and applying.
The water treatment agents prepared in example 1 and comparative example 3 were used for the cultivation water treatment, respectively, at an application rate of 2 kg/mu once a week for 1 month, as shown in FIG. 4. As can be seen from FIG. 4, the cultivation water treated in example 1 was clear (left panel), while the cultivation water treated in comparative example 3 was clear but cloudy (right panel).
Example 2
The following raw materials were weighed and mixed to obtain an amino acid medium, 18 parts of peptone, 10 parts of yeast extract, 2 parts of brown sugar, 8 parts of urea, 7 parts of sodium chloride, 0.8 part of tween 80, 0.5 part of glutamine, 0.7 part of histidine, 0.4 part of leucine, 0.4 part of methionine, 0.3 part of threonine, 0.3 part of valine, 0.2 part of aspartic acid, 0.3 part of glycine and 0.2 part of proline.
40 parts of amino acid culture medium, 35 parts of sucrose-derived fructo-oligosaccharide, 12 parts of EM flora, 5 parts of compound nitrifying bacteria, 2 parts of yeast selenium and 0.2 part of bacteroides parahaemolyticus are weighed and uniformly mixed.
Example 3
The following raw materials were weighed and mixed to obtain an amino acid medium, 22 parts of peptone, 6 parts of yeast extract, 8 parts of brown sugar, 12 parts of urea, 3 parts of sodium chloride, 1.2 parts of tween 80, 0.7 part of glutamine, 0.5 part of histidine, 0.6 part of leucine, 0.6 part of methionine, 0.5 part of threonine, 0.5 part of valine, 0.4 part of aspartic acid, 0.1 part of glycine and 0.1 part of proline.
Weighing 45 parts of amino acid culture medium, 30 parts of sucrose-derived fructo-oligosaccharide, 10 parts of EM flora, 4 parts of compound nitrifying bacteria, 3 parts of yeast selenium and 0.3 part of bacteroides parahaemolyticus, and uniformly mixing.
Experimental example 1
Experiment site: the experimental example is carried out on a certain culture base of Shandong jujube village.
Experimental time: 24 days of 4 months in 2019 to 5 days of 5 months in 2019.
Aquaculture water: grass carp fish.
In month 4 of 2019, the farmer finds that the fish is infected with diseases, the death rate of grass carp gradually increases from the beginning of April, and the death rate reaches 109 per day by 24 days of month 4 of 2019. The water treatment agent prepared in example 1 of the present invention was used without any problem. And records the death of grass carp as shown in table 1.
TABLE 1 mortality of grass carp after use of the Water quality treatment agent of the invention
| Date of day | Mortality rate of |
| 24 days of 4 months | 109 strips/day |
| 4 months and 28 days | 85 strips/day |
| Day 29 of 4 months | 60 strips/day |
| 4 months 30 days | 20 strips/day |
| 5 months 1 day | 20 strips/day |
| 5 months and 4 days | 7 strips/day |
| 5 months and 5 days | 5 strips/day |
As can be seen from the records in Table 1, the mortality rate was reduced from 109 to 85 per day on day 5 with the water treatment agent of the present invention, and then reduced every day until 5 months and 5 days, and the mortality rate was reduced by 5 per day, and was substantially restored to normal. Therefore, the water quality treating agent provided by the invention has remarkable effects of improving water quality and reducing the death rate of diseased fish.
In the above examples, the composite nitrifying bacteria were purchased from Henan fish Ying Jia Biotechnology Co., ltd, with a specification of 500 g/bag. The main components are nitrosated bacteria and nitrified bacteria, and the effective viable count is 10 hundred million/g.
The EM flora is purchased from Jinan Kaiyue chemical engineering Co., ltd, and the effective viable count is 1000 hundred million/g.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (1)
1. The use of a water treatment agent for purifying the water quality of aquaculture is characterized in that the water treatment agent comprises the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of compound nitrifying bacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides paraqualis;
the amino acid culture medium comprises the following raw materials in parts by weight: 18 to 22 parts of peptone, 6 to 10 parts of yeast extract, 8 to 12 parts of brown sugar, 8 to 12 parts of urea, 3 to 7 parts of sodium chloride, 0.8 to 1.2 parts of tween 80, 0.5 to 0.7 part of glutamine, 0.5 to 0.7 part of histidine, 0.4 to 0.6 part of leucine, 0.4 to 0.6 part of methionine, 0.3 to 0.5 part of threonine, 0.3 to 0.5 part of valine, 0.2 to 0.4 part of aspartic acid, 0.1 to 0.3 part of glycine and 0.1 to 0.3 part of proline;
the oligosaccharide is sucrose-derived fructo-oligosaccharide.
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