CN104118945B - A kind of compound micro-ecological preparation of degrading cultivation water nitrite and application - Google Patents
A kind of compound micro-ecological preparation of degrading cultivation water nitrite and application Download PDFInfo
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- CN104118945B CN104118945B CN201310147034.9A CN201310147034A CN104118945B CN 104118945 B CN104118945 B CN 104118945B CN 201310147034 A CN201310147034 A CN 201310147034A CN 104118945 B CN104118945 B CN 104118945B
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Abstract
The invention belongs to cultural technique field, the invention discloses a kind of compound micro-ecological preparation and application of degrading cultivation water nitrite, this probiotics raw material is streptococcus faecium, photosynthetic bacterium and denitrifying bacterium.This compound micro-ecological preparation, can reduce the hazardous and noxious substances such as minimizing ammonia nitrogen in water and nitrite fast, play Optimal culture environment, promotes the effects such as water surrounding benign ecological cycles, reduces the consumption to oxygen in aquaculture water; By adding composite auxiliary material, add the quality guaranteed period of probiotics of the present invention.
Description
Technical field
The invention belongs to cultural technique field, be specifically related to the probiotics containing streptococcus faecium, photosynthetic bacterium and denitrifying bacterium.
Background technology
In high-density intensive culture water body, have accumulated the movement etc. of the residual bait of a large amount of feeds and aquatic animal, pollute organism to increase, Water-environment Ecological System overbalance, nitrite severe overweight in water body, have impact on the healthy growth of aquatic animal, the reparation to aquaculture water environment must be accelerated.
The nitrite of high density easily causes unwanted bacteria and conditioned pathogen to grow in a large number, and the physique of the cultivated animals such as fish, shrimp declines, and anti-stress ability is poor, is subject to the invasion and attack of pathogenic bacteria, causes the big area of aquatic animal disease to break out.At present, ammonia nitrogen and nitrite concentration in water body is reduced mainly through methods such as physics, chemistry, microorganism and indirect rich water.Research deep of microbial process to microorganism in water body and cultivated animals probiotic bacterium grows up, be explore from ecological angle a kind ofly base oneself upon self-regeneration, long-acting, safe method, have nontoxic, have no side effect, noresidue, pollution-free, safe and reliable, the time length is long, the features such as steady water is effective, more and more receive the concern of aquaculture industry.
Aquaculture, as an artificial controlled process, is that microorganism such as photosynthetic bacterium, EM bacterium, nitrobacteria, genus bacillus, the lactobacillus etc. by introducing some amount artificially in water body environment carry out ammonia nitrogen, nitrite reparation.By using the good microbial preparation of biological activity, to accelerate organic oxidative nitration process in cultivating pool.Aquaculture water environment is a homestat be made up of multiple-microorganism inherently, probiotics and harmful bacteria coexist, the effect dominant of harmful bacteria when bad environments, this just needs to be added with beneficial microorganism to water body, the growth that dominant population suppresses harmful levels of pathogens is become by amount reproduction, simultaneously by beneficial microorganism metabolism, nutritive substance superfluous in water and the objectionable impurities such as ammonia nitrogen, nitrite can be reduced.
Market there are the microbial preparation products such as photosynthetic bacterium, genus bacillus, yeast, nitrobacteria, genus bacillus, milk-acid bacteria, apply this kind of microbial preparation product and can reduce water nitrite and ammonia-nitrogen content, removing Organic Hazardous Compounds, purifying water body.But all how much there is certain deficiency in these single microorganism preparations, breeding environment is complicated and changeable, need multiple different microorganisms preparation mutually to coordinate just to make whole breeding environment system be among ecological balanced system, the objectionable impuritiess such as the ammonia nitrogen in breeding environment, nitrite are in minimum level.In breeding environment ammonia nitrogen, nitrite biological restoration in, complex microorganism preparations just shows its good applied research prospect.
Summary of the invention
For these reasons, applicant is through practical studies for many years, develop a kind of new compound micro-ecological preparation, said preparation Raw is streptococcus faecium, photosynthetic bacterium and denitrifying bacterium, use this compound micro-ecological preparation, the hazardous and noxious substances such as ammonia nitrogen and nitrite in water can be reduced fast, play Optimal culture environment, promote the effects such as water surrounding benign ecological cycles, reduce the consumption to oxygen in aquaculture water; By adding composite auxiliary material, add the quality guaranteed period of probiotics of the present invention.
The present invention is achieved through the following technical solutions.
A kind of probiotics, said preparation Raw is streptococcus faecium, photosynthetic bacterium and denitrifying bacterium.
Above-mentioned preferred probiotics, wherein the weight ratio of streptococcus faecium, photosynthetic bacterium and denitrifying bacterium is 18 ~ 22:4 ~ 6:4 ~ 6.
Above-mentioned preferred probiotics, wherein the weight ratio of streptococcus faecium, photosynthetic bacterium and denitrifying bacterium is 20:5:5.
A kind of probiotics described above, wherein preparation comprises auxiliary material.
A kind of probiotics described above, wherein auxiliary material is zeolite powder, wilkinite and talcum powder.
Preferred auxiliary material zeolite powder, wilkinite and talcous weight ratio are 34 ~ 36:20 ~ 22:13 ~ 15.
Preferred auxiliary material zeolite powder, wilkinite and talcous weight ratio are 35:21:14.
Probiotics described above is in the application of aquaculture field.
Probiotics described above is for reducing the content of the nitrite in water body.
Streptococcus faecium of the present invention, photosynthetic bacterium, denitrifying bacterium are all purchased from the green world, Hubei bio tech ltd.
Embodiment
1, compound micro-ecological preparation shaker test
Test 1 group: streptococcus faecium 5g, photosynthetic bacterium 15g, denitrifying bacterium 10g.
Test 2 groups: streptococcus faecium 10g, photosynthetic bacterium 10g, denitrifying bacterium 10g.
Test 3 groups: streptococcus faecium 18g, photosynthetic bacterium 6g, denitrifying bacterium 6g.
Test 4 groups: streptococcus faecium 20g, photosynthetic bacterium 5g, denitrifying bacterium 5g.
Test 5 groups: streptococcus faecium 22g, photosynthetic bacterium 4g, denitrifying bacterium 4g.
Preparation method: get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add 35g zeolite powder, 21g wilkinite and 14g talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Test method: trial tank 2 mu, totally 6, the depth of water 1.5 meters, before test, hydrogen peroxide is thrown in each pond, and injected volume is 0.5L/ mu, above-mentioned test group compound micro-ecological preparation is thrown in after 3 days, consumption is 0.5kg/ mu, and control group trial tank does not drop into probiotics, 1 ~ 7 day synchronous Water quality detected.PH adopts pH meter directly to measure; Dissolved oxygen adopts portable dissolved oxygen analytic instrument to measure; NO
2 --N content adopts hydrochloric acid to be that quadrol method measures (with reference to GB5009.33-85); NH
3-N content adopts Berthelot spectrophotometry to measure (with reference to GB/T7479-1987).Test-results is in table 1 ~ 4.
Table 1 Analysis Results of Water Quality
Table 2 Analysis Results of Water Quality
Table 3 Analysis Results of Water Quality
Table 4 Analysis Results of Water Quality
Test-results shows, during test 3 ~ 5 groups of 7d, nitrite, ammonia nitrogen degradation rate all reach more than 60%, and wherein during the 4th group of 7d, nitrite degradation rate is up to 67.57%, and ammonia nitrogen degradation rate is up to 68.29%.During test 1 ~ 2 group of 7d, nitrite degradation rate all can reach more than 40%, but its effect is not as 3 ~ 5 groups, therefore gives up.
2, checking in probiotics practice
Test group: streptococcus faecium 20kg, photosynthetic bacterium 5kg, denitrifying bacterium 5kg.Preparation method: get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add 35kg zeolite powder, 21kg wilkinite and 14kg talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Trial tank 2 mu, the depth of water 1.5 meters, before test, hydrogen peroxide is thrown in each pond, and injected volume is 0.5L/ mu, throws in above-mentioned test group compound micro-ecological preparation after 3 days, and consumption is 0.50kg/ mu, 1 ~ 7 day synchronous Water quality detected.PH adopts pH meter directly to measure; Dissolved oxygen adopts portable dissolved oxygen analytic instrument to measure; NO
2 --N content adopts hydrochloric acid to be that quadrol method measures (with reference to GB5009.33-85); NH
3-N content adopts Berthelot spectrophotometry to measure (with reference to GB/T7479-1987).Test-results is in table 5.
Table 5 Analysis Results of Water Quality
Test-results shows, the NH in this compound micro-ecological preparation energy fast degradation water body
3-N and NO
2 -nH when-N, 7d
3-N degradation rate reaches 65.63%, NO
2 --N degradation rate can reach 68.29%, and it is less on Dissolved Oxygen in Water, pH impact.
3, probiotics stability test of the present invention
Test group: streptococcus faecium 20g, photosynthetic bacterium 5g, denitrifying bacterium 5g.Preparation method: get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add 35g zeolite powder, 21g wilkinite and 14g talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Above-mentioned probiotics, preserves, carries out live bacterial count every 30d to compound micro-ecological preparation product under normal temperature (20 ~ 35 DEG C) and low temperature (4 ~ 6 DEG C), accumulative detection 9 months.
Test-results: normal temperature lower 9 months (20 ~ 35 DEG C) living bacteria count survival rates can reach 85%.Preserve 9 months living bacteria count survival rates under low temperature (4 ~ 6 DEG C) and can 96% be reached.
Preparation embodiment
Embodiment 1
Probiotics raw material is: streptococcus faecium 18kg, photosynthetic bacterium 6kg, denitrifying bacterium 6kg.
Auxiliary material is: 34kg zeolite powder, 21kg wilkinite and 15kg talcum powder.
Get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add zeolite powder, wilkinite and talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Embodiment 2
Probiotics raw material is: streptococcus faecium 19kg, photosynthetic bacterium 6kg, denitrifying bacterium 5kg.
Auxiliary material is: 34.5kg zeolite powder, 20.5kg wilkinite and 15kg talcum powder.
Get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add zeolite powder, wilkinite and talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Embodiment 3
Probiotics raw material is: streptococcus faecium 20kg, photosynthetic bacterium 5kg, denitrifying bacterium 5kg.
Auxiliary material is: 35kg zeolite powder, 21kg wilkinite and 14kg talcum powder.
Get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add zeolite powder, wilkinite and talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Embodiment 4
Probiotics raw material is: streptococcus faecium 22kg, photosynthetic bacterium 4kg, denitrifying bacterium 4kg.
Auxiliary material is: 36kg zeolite powder, 21kg wilkinite and 13kg talcum powder.
Get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add zeolite powder, wilkinite and talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Embodiment 5
Probiotics raw material is: streptococcus faecium 21.5kg, photosynthetic bacterium 4.5kg, denitrifying bacterium 4kg.Auxiliary material is: 36kg zeolite powder, 21kg wilkinite and 13kg talcum powder.
Get streptococcus faecium, photosynthetic bacterium, denitrifying bacterium mix, add zeolite powder, wilkinite and talcum powder, after mixing, obtain the probiotics of above-mentioned test group.
Preparation embodiment includes but not limited to above-mentioned.
Claims (8)
1. a probiotics, is characterized in that said preparation Raw is streptococcus faecium, photosynthetic bacterium and denitrifying bacterium; Wherein the weight ratio of streptococcus faecium, photosynthetic bacterium and denitrifying bacterium is 18-22:4-6:4-6.
2. a kind of probiotics according to claim 1, wherein the weight ratio of streptococcus faecium, photosynthetic bacterium and denitrifying bacterium is 20:5:5.
3. a kind of probiotics according to claim 1, wherein preparation comprises auxiliary material.
4. a kind of probiotics according to claim 3, wherein auxiliary material is zeolite powder, wilkinite and talcum powder.
5. a kind of probiotics according to claim 4, wherein auxiliary material zeolite powder, wilkinite and talcous weight ratio are 34-36:20-22:13-15.
6. a kind of probiotics according to claim 4, wherein auxiliary material zeolite powder, wilkinite and talcous weight ratio are 35:21:14.
7. probiotics according to any one of claim 1-6, said preparation is for reducing the content of the nitrite in water body.
8. the probiotics according to any one of claim 1-6 is in the application in water cultivation field.
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| CN107828698B (en) * | 2017-12-01 | 2021-09-21 | 广州金水动物保健品有限公司 | Compound bacterium preparation and preparation method and application thereof |
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| CN101580306A (en) * | 2009-06-17 | 2009-11-18 | 辽宁大学 | Method for treating breeding water by composite bacteria |
| JP5935076B2 (en) * | 2012-03-15 | 2016-06-15 | 大成建設株式会社 | Water treatment equipment |
| CN102718327B (en) * | 2012-07-05 | 2013-11-06 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
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Denomination of invention: Composite microecological preparation for degrading nitrite in aquatic water and application thereof Effective date of registration: 20171214 Granted publication date: 20160120 Pledgee: Pudong Development Bank, Shanghai, Shanghai, Guangzhou Tianhe branch Pledgor: Guangzhou Jinshui Animal Health-Care Product Co., Ltd. Registration number: 2017440000184 |
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Date of cancellation: 20190312 Granted publication date: 20160120 Pledgee: Pudong Development Bank, Shanghai, Shanghai, Guangzhou Tianhe branch Pledgor: Guangzhou Jinshui Animal Health-Care Product Co., Ltd. Registration number: 2017440000184 |