CN115745195A - Water quality treating agent for aquaculture and application thereof - Google Patents
Water quality treating agent for aquaculture and application thereof Download PDFInfo
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- CN115745195A CN115745195A CN202211597074.9A CN202211597074A CN115745195A CN 115745195 A CN115745195 A CN 115745195A CN 202211597074 A CN202211597074 A CN 202211597074A CN 115745195 A CN115745195 A CN 115745195A
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Abstract
The invention provides a water quality treating agent for aquaculture and application thereof, and belongs to the technical field of water quality treatment. The invention provides a water quality treating agent for aquaculture, which comprises the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of composite nitrobacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides parahaemolyticus. The water quality treating agent provided by the invention has the effects of improving water quality, increasing oxygen content of water and reducing death rate of diseased fish. The method is used in the field of aquaculture, and can obviously improve the quality and yield of aquatic products.
Description
Technical Field
The invention relates to the technical field of water quality treatment agents, in particular to a water quality treatment agent for aquaculture and application thereof.
Background
With the development of economic society, the requirements of people on the quantity and quality of aquatic products are higher and higher, in order to pursue high benefits, the stocking density is increased blindly, so that the aquaculture is developed to high density, intensification and industrialization, and the bait and antibiotic put-in amount is also larger and larger due to the increase of the aquaculture scale. The water pollution is aggravated, and serious consequences such as water anoxia, killing of beneficial microorganisms, frequent occurrence of fish and shrimp diseases and the like are brought. Restricting the growth of fish and shrimp, resulting in reduced yield. Therefore, in the existing aquaculture process, the water quality treatment agent is used for sterilizing and disinfecting water, improving the transparency of the water body, reducing the content of ammonia nitrogen in the water body, reducing the chemical oxygen consumption and the biochemical oxygen consumption and the like to improve the aquaculture environment.
The biological water purifying agent is a kind of microecological preparation containing bacillus, enzyme and nutrient substance, and may be used in various links of intensive aquatic product culture. The substances such as filth, organic sediment, ammonia, nitrogen and the like in the culture pond can be degraded through the action of microorganisms and enzymes, so that organic waste is liquefied, and the water quality is purified. The microorganism in the biological water purifying agent is used as an important activity and transformation carrier, and plays a role in water purification. The commonly used microbial agent is a complex microbial agent, for example, chinese patent CN101475265B provides a complex microbial agent, and complex microbial agents I-III are coordinated and complemented to achieve the water purification effect. However, as the strain components and the physicochemical characteristics of the composite microbial preparation are different, the water quality condition and the pollution degree of the purified water body are different, so that the purification effect of the microbial preparation is greatly different.
The nutrient substances are the basis for the survival and reproduction of the microbial inoculum, but different nutrient substances have different influences on the water purification capacity of the microbial inoculum. For example, commonly added nutrients include methanol, ethanol, acetic acid, sodium acetate, glucose, phosphorus, and the like. The research proves that the additional glucose can improve the nitrification efficiency and the purification capacity of microorganisms, and the study of scholars shows that the phosphorus deficiency can influence the purification effect of the water quality purification microbial inoculum.
The invention provides a water quality treating agent for aquaculture.
Disclosure of Invention
The invention aims to provide a water quality treating agent for aquaculture, which is used for improving water quality, increasing oxygen content of water, reducing the average mortality rate of aquatic products and increasing yield.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a water quality treating agent for aquaculture, which comprises the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of composite nitrobacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides parahaemolyticus.
Preferably, the amino acid culture medium comprises the following raw materials in parts by weight: 18 to 22 portions of peptone, 6 to 10 portions of yeast extract, 8 to 12 portions of brown sugar, 8 to 12 portions of urea, 3 to 7 portions of sodium chloride, 0.8 to 1.2 portions of tween, 0.5 to 0.7 portion of glutamine, 0.5 to 0.7 portion of histidine, 0.4 to 0.6 portion of leucine, 0.4 to 0.6 portion of methionine, 0.3 to 0.5 portion of threonine, 0.3 to 0.5 portion of valine, 0.2 to 0.4 portion of aspartic acid, 0.1 to 0.3 portion of glycine and 0.1 to 0.3 portion of proline.
Preferably, the oligosaccharide is one or more of fructo-oligosaccharide, lacto-oligosaccharide and isomaltose.
Preferably, the fructooligosaccharide is inulin-derived fructooligosaccharide or sucrose-derived fructooligosaccharide.
The invention also provides the application of the water quality treating agent in purifying the water quality of aquaculture.
The water quality treating agent provided by the invention has the effects of improving water quality, increasing oxygen content of water and reducing death rate of diseased fish. The method is used in the field of aquaculture, the improved water quality is clear and transparent, the oxygen content can be increased from 10.12mg/L to 24.38mg/L, the method belongs to high-quality water areas of aquaculture, and the method is used for purifying the water quality of diseased fish, so that the death rate of the diseased fish can be greatly reduced, the quality and the yield of aquatic products can be obviously improved, and the loss of farmers can be reduced.
Drawings
FIG. 1 is a comparative graph showing the improvement in water quality before and after the treatment with the water treatment agent in example 1;
FIG. 2 is a comparison result of two water quality treatment agents in example 2;
FIG. 3 is a comparison result of two water quality treatment agents in example 3;
FIG. 4 is a comparison result of two water treatment agents in example 4.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The raw materials are weighed according to the following concentration and mixed to obtain an amino acid culture medium, wherein the amino acid culture medium comprises 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 80 parts of tween, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
Weighing 43 parts of amino acid culture medium, 32 parts of sucrose-derived fructo-oligosaccharide, 11 parts of EM (effective microorganism) flora, 4 parts of composite nitrobacteria, 2 parts of yeast selenium and 0.3 part of bacteroides paradoxus, and uniformly mixing for application.
The water treatment agent prepared in example 1 was tested in an aquaculture site at the bottom of the Bizhen of Yichen district of jujube village, city, shandong province. The application amount is 2 kg/mu once a week. After one month observation, the results are shown in FIG. 1. As can be seen from FIG. 1, the water quality before use is yellow-green and has a large amount of moss. The water quality is clear and has no moss after one month of use, and the method is suitable for aquaculture. The oxygen content of the water body before and after use is measured, and is 10.12mg/L before use and 24.38mg/L after use. Therefore, the water quality treating agent provided by the invention has the effects of improving water quality and increasing oxygen content of water.
Comparative example 1
The difference between the comparative example and the example 1 is that the used culture medium is a common culture medium, and the component ratio is as follows: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 80 parts of tween and 0.8 part of cysteine.
The formula of the water quality treating agent is as follows: 43 parts of common culture medium, 32 parts of sucrose-derived fructo-oligosaccharide, 11 parts of EM (effective microorganism) flora, 4 parts of composite nitrobacteria, 2 parts of yeast selenium and 0.3 part of bacteroides paradoxus, and the components are uniformly mixed and then applied.
The water quality treatment agents prepared in example 1 and comparative example 1 were used for treatment of aquaculture water at an application rate of 2 kg/acre once per week after 2 months, respectively, as shown in fig. 2. As is evident from FIG. 2, the water of the treated aquaculture water of example 1 is clear (right panel). The water treated in comparative example 1 was turbid, yellow-green in color, and a small amount of moss was present (left panel).
Comparative example 2
This comparative example differs from example 1 in that glucose is used instead of fructooligosaccharides (a source of sucrose).
Amino acid culture medium: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 80 parts of tween, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
The formula of the water quality treating agent comprises: 43 parts of amino acid culture medium, 32 parts of glucose, 11 parts of EM flora, 4 parts of composite nitrobacteria, 2 parts of selenium yeast and 0.3 part of bacteroides parahaemolyticus, and the components are uniformly mixed and applied
The water quality treatment agents prepared in example 1 and comparative example 2 were used for water quality treatment of a rice-crab symbiotic system at an application rate of 1.5 kg/mu once a week after 1 month, respectively, as shown in fig. 3. As can be seen from fig. 3, the water quality of the symbiotic system of rice and crab treated in example 1 is clear without moss impurities (left panel), while the water quality of the symbiotic system of rice and crab treated in comparative example 2 is crossed and a large amount of moss exists (right panel).
Comparative example 3
This comparative example differs from example 1 in that the oligofructose derived from sucrose in example 1 was replaced by oligofructose derived from inulin.
Amino acid culture medium: 20 parts of peptone, 8 parts of yeast extract, 10 parts of brown sugar, 10 parts of urea, 5 parts of sodium chloride, 80 parts of tween, 0.6 part of glutamine, 0.6 part of histidine, 0.5 part of leucine, 0.5 part of methionine, 0.4 part of threonine, 0.4 part of valine, 0.3 part of aspartic acid, 0.2 part of glycine and 0.3 part of proline.
The formula of the water quality treating agent comprises: 43 parts of amino acid culture medium, 32 parts of inulin-derived fructo-oligosaccharide, 11 parts of EM (effective microorganism) flora, 4 parts of composite nitrobacteria, 2 parts of yeast selenium and 0.3 part of bacteroides parahaemolyticus, and the components are uniformly mixed and applied.
The water quality treatment agents prepared in example 1 and comparative example 3 were used for treatment of aquaculture water at an application rate of 2 kg/acre once per week after 1 month, respectively, as shown in FIG. 4. As can be seen from FIG. 4, the quality of the culture water treated in example 1 is clear (left panel), while the culture water treated in comparative example 3 has no moss but has turbid water (right panel).
Example 2
The raw materials are weighed according to the following concentration and mixed to obtain an amino acid culture medium, wherein the amino acid culture medium comprises 18 parts of peptone, 10 parts of yeast extract, 2 parts of brown sugar, 8 parts of urea, 7 parts of sodium chloride, 0.8 part of tween 80, 0.5 part of glutamine, 0.7 part of histidine, 0.4 part of leucine, 0.4 part of methionine, 0.3 part of threonine, 0.3 part of valine, 0.2 part of aspartic acid, 0.3 part of glycine and 0.2 part of proline.
Weighing 40 parts of amino acid culture medium, 35 parts of sucrose source fructo-oligosaccharide, 12 parts of EM flora, 5 parts of composite nitrobacteria, 2 parts of yeast selenium and 0.2 part of bacteroides parahaemolyticus, and uniformly mixing.
Example 3
The raw materials are weighed according to the following concentration, and mixed to obtain an amino acid culture medium, 22 parts of peptone, 6 parts of yeast extract, 8 parts of brown sugar, 12 parts of urea, 3 parts of sodium chloride, 1.2 parts of tween, 0.7 part of glutamine, 0.5 part of histidine, 0.6 part of leucine, 0.6 part of methionine, 0.5 part of threonine, 0.5 part of valine, 0.4 part of aspartic acid, 0.1 part of glycine and 0.1 part of proline.
Weighing 45 parts of amino acid culture medium, 30 parts of sucrose-derived fructo-oligosaccharide, 10 parts of EM (effective microorganism) flora, 4 parts of composite nitrobacteria, 3 parts of yeast selenium and 0.3 part of bacteroides paradoxus, and uniformly mixing.
Experimental example 1
The experimental site: the experimental example is carried out in a certain cultivation base of Shandong jujube village.
Experiment time: 24/2019 on month 4 to 5/2019 on month 5.
Aquaculture: grass carp.
In the 4 th month of 2019, the farmer finds that the fish pond is infected with diseases, the mortality rate of the grass carps gradually increases from the beginning of the fourth month, and the mortality rate reaches 109 per day by 24 th 4 th month of 2019. The water treatment agent prepared in example 1 of the present invention was used without any problem. And grass carp deaths were recorded as shown in table 1.
TABLE 1 mortality of grass carp after using the water treatment agent of the present invention
| Date | Mortality rate |
| 4 month and 24 days | 109 strips/day |
| 4 month and 28 days | 85 strips/day |
| 4 month and 29 days | 60 strips/day |
| 4 month and 30 days | 20 strips/day |
| 5 months and 1 day | 20 strips/day |
| 5 months and 4 days | 7 strips/day |
| 5 months and 5 days | 5 strips/day |
As can be seen from the record in Table 1, the mortality rate decreased from 109 to 85 on day 5 using the water treatment agent of the present invention, and then decreased day by day until 5 months and 5 days, and the mortality rate decreased by 5 strips/day and substantially returned to normal. Therefore, the water quality treating agent provided by the invention has obvious effects of improving water quality and reducing death rate of diseased fish.
In the above examples, the composite nitrifying bacteria were purchased from Henan fish Yijia Biotechnology Ltd, with a specification of 500 g/bag. The main components are nitrosobacteria and nitrobacteria, and the number of effective viable bacteria is 10 hundred million/g.
The EM flora is purchased from Jinan Shi chemical technology Co., ltd, and the number of effective viable bacteria is 1000 hundred million/g.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A water quality treating agent for aquaculture is characterized by comprising the following raw materials in parts by weight: 40-45 parts of amino acid culture medium, 30-35 parts of oligosaccharide, 10-12 parts of EM flora, 4-5 parts of composite nitrobacteria, 2-3 parts of yeast selenium and 0.2-0.3 part of bacteroides parahaemolyticus.
2. The water quality treating agent according to claim 1, wherein the amino acid culture medium comprises the following raw materials in parts by weight: 18 to 22 portions of peptone, 6 to 10 portions of yeast extract, 8 to 12 portions of brown sugar, 8 to 12 portions of urea, 3 to 7 portions of sodium chloride, 0.8 to 1.2 portions of tween, 0.5 to 0.7 portion of glutamine, 0.5 to 0.7 portion of histidine, 0.4 to 0.6 portion of leucine, 0.4 to 0.6 portion of methionine, 0.3 to 0.5 portion of threonine, 0.3 to 0.5 portion of valine, 0.2 to 0.4 portion of aspartic acid, 0.1 to 0.3 portion of glycine and 0.1 to 0.3 portion of proline.
3. The water quality treatment agent according to claim 1 or 2, wherein the oligosaccharide is one or more of fructooligosaccharide, lactooligosaccharide and isomaltose.
4. A water treatment agent as defined in claim 3 wherein said fructooligosaccharide is an inulin-derived fructooligosaccharide or a sucrose-derived fructooligosaccharide.
5. Use of the water quality treatment agent according to any one of claims 1 to 4 for purifying aquaculture water.
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