CN109168415A - A method of improving dalbergia odorifera percentage of seedgermination - Google Patents
A method of improving dalbergia odorifera percentage of seedgermination Download PDFInfo
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- CN109168415A CN109168415A CN201811284046.5A CN201811284046A CN109168415A CN 109168415 A CN109168415 A CN 109168415A CN 201811284046 A CN201811284046 A CN 201811284046A CN 109168415 A CN109168415 A CN 109168415A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of methods for improving dalbergia odorifera percentage of seedgermination, pass through KNO3Synergistic effect between multiple committed steps such as solution seed soaking, the seed soaking of garlic leachate, the combination of different Germination textures, germinates after realizing dalbergia odorifera seed culture 5 days, and germination percentage reaches 87% or so after 9 days, and germination percentage is up to 96.3% after culture 19 days.The method of the present invention is simple and easy to do, favorable repeatability, effect are good, is suitble to large-scale promotion application.
Description
Technical field
The present invention relates to a kind of methods for improving dalbergia odorifera percentage of seedgermination, belong to field of plant growing technology.
Background technique
Dalbergia odorifera Dalbergia odoriferaT.Chen. is pulse family Papillionoideae yellow wingceltis platymiscium, alias flower
Pears, dalbergia wood wingceltis etc. are China's endemic trees, and province of Hainan Province tree, its field kind is in pole Critical Condition at present, are listed in country two
Grade protected trees.Its heartwood pole corrosion resistant is the choice wood of the rare furniture of production, craftwork etc., dalbergia odorifera sea originating in China
Nan Sheng, West Hainan Li Autonomous County of ledong, Dongfang Shi, Sanya, at present Guangdong, Guangxi, Fujian, Yunnan, Sichuan, Chongqing etc.
There are introducing and planting in provinces and cities.It differs greatly due to introducing a fine variety region weather conditions at present, percentage of seedgermination is irregular, and it is big to influence it
Area cultivation is introduced a fine variety, therefore provides a kind of raising dalbergia odorifera percentage of seedgermination and method simple and easy to do, easy to spread has very much
It is necessary.
Presently relevant technology is as follows: yellow soil is higher than the germination percentage of river sand, peat soil as sowing media, can achieve
72.7%.(Li Xiaowen, the influence that different ratio matrix sprouts dalbergia odorifera seed).In the prior art, dalbergia odorifera seed
Although there is very big raising space up to 72% in germination percentage.
Summary of the invention
In consideration of it, the present invention provides a kind of method for improving dalbergia odorifera percentage of seedgermination, dalbergia odorifera seed hair is solved
Bud rate is not high, is unfavorable for the problem of being widely applied of dalbergia odorifera cultivation.
The technical solution adopted by the present invention is as follows:
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm dalbergia odorifera seed is selected, initial moisture content is measured, carries out disinfection, be washed with water
Only；
2) seed is placed in the KNO of mass percent concentration 2~4%312~36h is impregnated in solution, is cleaned, constant temperature drying
To initial moisture content；
3) seed is put into concentration is 15~35mgL-18~10h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 10~30min of processing in 40~60 DEG C of warm water；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are at least one of yellow soil, coco bran and river sand；
6) culture dish is placed in vernalization in insulating box.
Preferably, KNO3The mass percent concentration of solution is 4%.
Preferably, seed is in KNO3Soaking time in solution is for 24 hours.
Preferably, the concentration of garlic extraneous liquid is 20mgL-1。
Preferably, the time that seed impregnates in garlic extraneous liquid is 8h.
Preferably, garlic extraneous liquid the preparation method comprises the following steps:
It by garlic peeling, mashes, the dehydrated alcohol that 1~3 times of its weight is added extracts, and centrifugation takes supernatant.
Preferably, the method for disinfection is 25min in the liquor potassic permanganate that seed is immersed in mass concentration and is 0.3%.
Preferably, in step 5), matrix is the mixture of yellow soil, coco bran and river sand, the matter of yellow soil, coco bran and river sand
Amount is than being (1~2): (1~2): (1~2).
Preferably, the mass ratio of yellow soil, coco bran and river sand is 2:1:1.
Compared with prior art, the beneficial effects of the present invention are:
The present invention passes through KNO3Multiple key steps such as solution seed soaking, the seed soaking of garlic leachate, the combination of different Germination textures
Synergistic effect between rapid is, it can be achieved that dalbergia odorifera seed culture is germinateed after 5 days, and germination percentage reaches 87% or so after 9 days, culture
Germination percentage is up to 96.3% after 19 days.
The method of the present invention is simple and easy to do, favorable repeatability, effect are good, is suitble to large-scale promotion application.
Specific embodiment
Below by specific embodiment, invention is further described in detail.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
Embodiment 1
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm 80 pieces of dalbergia odorifera seed (completely retaining its wing pod) is selected, original contain is measured
Seed is immersed in the liquor potassic permanganate that mass concentration is 0.3% and sterilizes 25min, is eluted with water by water；
2) seed is placed in the KNO of mass percent concentration 4%3It impregnates for 24 hours, takes out in solution, clean, 30 DEG C of constant temperature bakings
It does to initial moisture content；
3) seed is put into concentration is 20mgL-18h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 50 DEG C of warm water and handles 15min；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are that (0.3-2.0 meters of deep cubsoils have yellow soil span yellow soil from the ground for the red soil of limestone development
Certain viscosity, pH value 4~6 smash sieving), the mixture (mass ratio 2:1:1) of coco bran and river sand；
6) culture dish is placed in vernalization in 25-30 DEG C of insulating box.There is seed to start to sprout after 5 days, there are 70 pieces of seeds after 9 days
Prominent break in the seed coat of plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 77 pieces of seed germs to show money or valuables one carries unintentionally after 19 days, has 77 pieces of seed germs to break through kind after 29 days
Skin shows money or valuables one carries unintentionally.
Garlic extraneous liquid the preparation method comprises the following steps: by garlic peeling, mash, be added equal mass dehydrated alcohol carry out
It extracts, centrifugation takes supernatant.
Embodiment 2
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm 80 pieces of dalbergia odorifera seed (completely retaining its wing pod) is selected, original contain is measured
Seed is immersed in the liquor potassic permanganate that mass concentration is 0.3% and sterilizes 25min, is eluted with water by water；
2) seed is placed in the KNO of mass percent concentration 2%312h in solution takes out, and cleans, 30 DEG C of constant temperature are dried to
Initial moisture content；
3) seed is put into concentration is 15mgL-18h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 40 DEG C of warm water and handles 10min；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are the mixture of yellow soil, coco bran and river sand, and the mass ratio of yellow soil, coco bran and river sand is 2:1:1；
6) culture dish is placed in vernalization in 25-30 DEG C of insulating box.There is seed to start to sprout after 7 days, there are 65 pieces of kinds after 10 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 74 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 74 pieces of seed germs to break through after 30 days
Kind skin shows money or valuables one carries unintentionally.
The preparation method of garlic extraneous liquid is same as Example 1.
Embodiment 3
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm 80 pieces of dalbergia odorifera seed (completely retaining its wing pod) is selected, original contain is measured
Seed is immersed in the liquor potassic permanganate that mass concentration is 0.3% and sterilizes 25min, is eluted with water by water；
2) seed is placed in the KNO of mass percent concentration 4%336h in solution takes out, and cleans, 30 DEG C of constant temperature are dried to
Initial moisture content；
3) seed is put into concentration is 35mgL-110h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 60 DEG C of warm water and handles 30min；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are the mixture of yellow soil, coco bran and river sand, and the mass ratio of yellow soil, coco bran and river sand is 2:1:1；
6) culture dish is placed in vernalization in 25-30 DEG C of insulating box.There is seed to start to sprout after 7 days, there are 66 pieces of kinds after 10 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 72 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 72 pieces of seed germs to break through after 30 days
Kind skin shows money or valuables one carries unintentionally.
The preparation method of garlic extraneous liquid is same as Example 1.
Embodiment 4
Embodiment 4 and the difference of embodiment 3 be,
Garlic extraneous liquid the preparation method comprises the following steps: by garlic peeling, mash, be added the dehydrated alcohol of 3 times of its weight into
Row extracts, and centrifugation takes supernatant.
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, has seed to start to sprout after 7 days, there are 66 pieces of kinds after 10 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 73 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 73 pieces of seed germs to break through after 30 days
Kind skin shows money or valuables one carries unintentionally.
Embodiment 5
Embodiment 5 and the difference of embodiment 3 are, in step 5), matrix is the mixture of yellow soil, coco bran and river sand,
The mass ratio of yellow soil, coco bran and river sand is 1:1:2.
Culture dish is placed in vernalization in 25 DEG C of insulating box, has seed to start to sprout after 7 days, there are 65 pieces of seed embryos after 10 days
Prominent break in the seed coat of bud shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 68 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 68 pieces of seed germs are prominent to break in the seed coat after 30 days
It shows money or valuables one carries unintentionally.
Embodiment 6
Embodiment 6 and the difference of embodiment 3 are, in step 5), matrix is the mixture of yellow soil, coco bran and river sand,
The mass ratio of yellow soil, coco bran and river sand is 1:2:1.
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, has seed to start to sprout after 7 days, there are 66 pieces of kinds after 10 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 72 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 72 pieces of seed germs to break through after 30 days
Kind skin shows money or valuables one carries unintentionally.
Embodiment 7
Embodiment 7 and the difference of embodiment 3 are, in step 5), matrix is the mixture of yellow soil, coco bran and river sand,
The mass ratio of yellow soil, coco bran and river sand is 1:1:1.
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, has seed to start to sprout after 7 days, there are 65 pieces of kinds after 10 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 70 pieces of seed germs to show money or valuables one carries unintentionally after 20 days, has 70 pieces of seed germs to break through after 30 days
Kind skin shows money or valuables one carries unintentionally.
Embodiment 8
Embodiment 8 and the difference of embodiment 3 are, in step 5), matrix is yellow soil.
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, has seed to start to sprout after 9 days, there are 62 pieces of kinds after 12 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 66 pieces of seed germs to show money or valuables one carries unintentionally after 22 days, has 66 pieces of seed germs to break through after 32 days
Kind skin shows money or valuables one carries unintentionally.
Embodiment 9
Embodiment 9 and the difference of embodiment 3 are, in step 1), the method for disinfection is that seed is immersed in mass concentration
For 25min in 3% liquor potassic permanganate.
Culture dish is placed in vernalization in 25 DEG C of insulating box, has seed to start to sprout after 8 days, there are 63 pieces of seed embryos after 11 days
Prominent break in the seed coat of bud shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 65 pieces of seed germs to show money or valuables one carries unintentionally after 21 days, has 65 pieces of seed germs are prominent to break in the seed coat after 31 days
It shows money or valuables one carries unintentionally.
Comparative example 1
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm 80 pieces of dalbergia odorifera seed (completely retaining its wing pod) is selected, original contain is measured
Seed is immersed in the liquor potassic permanganate that mass concentration is 0.3% and sterilizes 25min, is eluted with water by water；
2) seed is placed in the KNO of mass percent concentration 1%3In solution for 24 hours, it takes out, cleans, 30 DEG C of constant temperature are dried to
Initial moisture content；
3) seed is put into concentration is 10mgL-18h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 35 DEG C of warm water and handles 15min；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are the mixture of yellow soil, coco bran and river sand, and the mass ratio of yellow soil, coco bran and river sand is 2:1:1；
6) culture dish is placed in vernalization in 25-30 DEG C of insulating box.There is seed to start to sprout after 10 days, there are 60 pieces of kinds after 12 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 63 pieces of seed germs to show money or valuables one carries unintentionally after 22 days, has 63 pieces of seed germs to break through after 32 days
Kind skin shows money or valuables one carries unintentionally.
The preparation method of garlic extraneous liquid is same as Example 1.
Comparative example 2
A method of improving dalbergia odorifera percentage of seedgermination, comprising the following steps:
1) mature, full, no disease and pests harm 80 pieces of dalbergia odorifera seed (completely retaining its wing pod) is selected, original contain is measured
Seed is immersed in the liquor potassic permanganate that mass concentration is 0.3% and sterilizes 25min, is eluted with water by water；
2) seed is placed in the KNO of mass percent concentration 5%3In solution for 24 hours, it takes out, cleans, 30 DEG C of constant temperature are dried to
Initial moisture content；
3) seed is put into concentration is 40mgL-18h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 70 DEG C of warm water and handles 15min；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keeps soil humidity, but cannot
Ponding, mesostroma are the mixture of yellow soil, coco bran and river sand, and the mass ratio of yellow soil, coco bran and river sand is 2:1:1；
6) culture dish is placed in vernalization in 25-30 DEG C of insulating box.There is seed to start to sprout after 10 days, there are 61 pieces of kinds after 12 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 63 pieces of seed germs to show money or valuables one carries unintentionally after 22 days, has 63 pieces of seed germs to break through after 32 days
Kind skin shows money or valuables one carries unintentionally.
The preparation method of garlic extraneous liquid is same as Example 1.
Comparative example 3
Comparative example 3 the difference from embodiment 1 is that, do not carry out step 3).
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, has seed to start to sprout after 13 days, there are 60 pieces of kinds after 15 days
Prominent break in the seed coat of sub- plumule shows money or valuables one carries unintentionally, and has prominent break in the seed coat of 62 pieces of seed germs to show money or valuables one carries unintentionally after 23 days, has 62 pieces of seed germs to break through after 33 days
Kind skin shows money or valuables one carries unintentionally.
Test example: germination percentage test
Culture dish is placed in vernalization in 25-30 DEG C of insulating box, observes and records germinative number during germination daily.With kind of a skin dew
The white mark for germination sent out end mark as seed without germination using continuous 10 days.Germination percentage=(normal germination
Seed number/confession plant experimentally subnumber) × 100%；It the results are shown in Table 1.
Table 1
From test result it is found that being sent out after 9 days using the method for the present invention, it can be achieved that dalbergia odorifera seed culture is germinateed after 5 days
Bud rate reaches 87% or so, and germination percentage is up to 96.3% after culture 19 days.
The above content is specific embodiment is combined, further detailed description of the invention, and it cannot be said that this hair
Bright specific implementation is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, it is not taking off
Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made.
Claims (9)
1. a kind of method for improving dalbergia odorifera percentage of seedgermination, which comprises the following steps:
1) mature, full, no disease and pests harm dalbergia odorifera seed is selected, initial moisture content is measured, carries out disinfection, be eluted with water；
2) seed is placed in the KNO of mass percent concentration 2~4%312~36h is impregnated in solution, is cleaned, constant temperature drying to original
Beginning water content；
3) seed is put into concentration is 15~35mgL-18~10h is impregnated in garlic extraneous liquid, is taken out；
4) dalbergia odorifera seed is placed in 10~30min of processing in 40~60 DEG C of warm water；
5) seed is placed in the culture dish containing culture substrate, covers seed with matrix, keep soil humidity, but not energy product
Water, mesostroma are at least one of yellow soil, coco bran and river sand；
6) culture dish is placed in vernalization in insulating box.
2. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that KNO3The matter of solution
Measuring percent concentration is 4%.
3. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that seed is in KNO3It is molten
Soaking time in liquid is for 24 hours.
4. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that garlic extraneous liquid
Concentration is 20mgL-1。
5. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that seed is soaked in garlic
The time impregnated in extract is 8h.
6. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that garlic extraneous liquid
The preparation method comprises the following steps:
It by garlic peeling, mashes, the dehydrated alcohol that 1~3 times of its weight is added extracts, and centrifugation takes supernatant.
7. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that in step 1), disappear
The method of poison is 25min in the liquor potassic permanganate that seed is immersed in mass concentration and is 0.3%.
8. the method according to claim 1 for improving dalbergia odorifera percentage of seedgermination, which is characterized in that in step 5), base
Matter is the mixture of yellow soil, coco bran and river sand, and the mass ratio of yellow soil, coco bran and river sand is (1~2): (1~2): (1~
2)。
9. the method for dalbergia odorifera percentage of seedgermination according to claim 8, which is characterized in that cubsoil, coco bran and river sand
Mass ratio be 2:1:1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110612868A (en) * | 2019-10-25 | 2019-12-27 | 海南省共一园农业科技有限公司 | Organic strawberry planting method |
| CN115226434A (en) * | 2022-08-09 | 2022-10-25 | 陕西师范大学 | Method for shortening germination period of thesium chinense seeds and improving germination rate |
| CN115226434B (en) * | 2022-08-09 | 2024-01-16 | 陕西师范大学 | Method for shortening germination period of bastardtoad seed and improving germination rate |
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