CN107683652A - A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination - Google Patents
A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination Download PDFInfo
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- CN107683652A CN107683652A CN201710894293.6A CN201710894293A CN107683652A CN 107683652 A CN107683652 A CN 107683652A CN 201710894293 A CN201710894293 A CN 201710894293A CN 107683652 A CN107683652 A CN 107683652A
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- seed
- chinese scholar
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination, step is：Step 1, seed is gathered：The seed for maturation of growing is chosen from Chinese scholar tree original producton location；Step 2, disinfection seed：After seed is warming up into room temperature, soaked in alcohol, then with disinfecting solution of potassium permanganate, then with aseptic water washing, blot the moisture of the surface of the seed, then with cutting kind of one osculum of skin, uniform broadcasting is to sprouting in culture dish；Step 3, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed, soaking at room temperature, obtains the Chinese scholar tree seed of imbibition；Step 4, the husky seed for broadcasting imbibition：Sand basin is established, sand basin fills clean river sand, and uniform broadcasting seed then covers seed on sand basin face in layer overlay river sand on seed face；Step 5, upper cup is heeled in：Seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.The advantages that present invention has low cost, and technology is simple and practical, and application value is high.
Description
Technical field
The invention belongs to plant seed germination nursery field, and in particular to one kind improves ornamental plant Chinese scholar tree percentage of seedgermination
Method.
Background technology
Chinese scholar tree (Sophora japonica) also known as family Chinese scholartree, Bai Huai, it is Papilionaceae Sophora deciduous tree.Tree performance is graceful,
Tree crown is circular, and hat is big shady dense, to the pernicious gases such as carbon dioxide, chlorine, hydrogen chloride and the resistance of flue dust, in addition, its life-span
Length, material are hard, strong adaptability, and sophora bud economic value is high, are northern China urban afforestation shade tree and are set with the excellent native soil of material
Kind.
There is Chinese scholar tree seed coat structure legume typically to plant skin feature.Kind skin is formed by outer Ovule Development, and interior ovule exists
Degenerate and disappear before seed development.Kind of skin is followed successively by parenchyma cell layer in irregular shape from inside to outside, iuntercellular has more space
Osteosclereide layer, very thick palisade layer, have angleplied laminate top layer.The parenchyma cell layer of kind endo-endothelial layer is inlayed tightly with endosperm
It is close, and the existing outermost layer of embryo deposits the very high galactomannan gum of viscosity, this be probably cause the kind skin of mature seed with
Endosperm is difficult the reason for separating.Ripe Chinese scholar tree seed, its kind of skin palisade layer is flourishing, and degree of lignification is high, and arrangement is close, top layer
There is fluid-tight wax coat, so as to cause seed water imbibition poor, germination rate is low.
At present in the world have Chinese scholar tree seed sprout nursery document report, but exist germination rate it is unstable, emergence
Irregular problem, the present invention can provide the husky key technology for broadcasting sprouting emergence in subset for Chinese scholar tree industrial seedling rearing.Its kind
Sub- sterilization method, physics and chemical method handle unique and simple material benefit, and application value is high.
The content of the invention
It is an object of the invention to provide a kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination, to improve Chinese scholar tree seed
Germination percentage.
A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination, comprises the following steps：
Step 1, seed is gathered：The seed for maturation of growing is chosen from Chinese scholar tree original producton location, is placed in protecting in 4 DEG C of refrigerators
Deposit；
Step 2, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, soaked in alcohol,
Disinfecting solution of potassium permanganate is used again, then with aseptic water washing, is blotted under aseptic condition on superclean bench with aseptic filter paper
The moisture of the surface of the seed, kind of one osculum of skin then is cut with aseptic operation knife, uniform broadcasting is into sprouting culture dish；
Step 3, seed treatment soaks：Pour into seed treatment into each culture dish for being covered with seed, soaking at room temperature,
Obtain the Chinese scholar tree seed of imbibition；
Step 4, the husky seed for broadcasting imbibition：It is that sand basin is established in 90% cool canopy in shading rate, sand basin fills clean river sand, river
Husky water content is 20-25%, and uniform broadcasting seed then covers kind on sand basin face in layer overlay river sand on seed face
Son；
Step 5, upper cup is heeled in：Using plastic cup, matrix is red soil, high 10-15 lis when Chinese scholar tree grows to two panels true leaf
Meter Shi, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
In the step 1, the method for choosing the seed for maturation of growing is：From In Nanjing Chinese scholar tree plant cultivation base
The strong Chinese scholar tree strain of robust growth, strong stress resistance, disease and insect resistance is chosen as seed collecting plant, with the mellow fruit of hand getting collection Chinese scholar tree
It is real.
In the step 1, after selected seed, seed is handled as follows：Seed is fitted into ventilative seed collecting bag, from
So dry, Fruit pod is gone in stranding, after exposure seed 6-24 hours in the sun, the storage of seeds moisture controlled in 9%-12%, satisfying
Full seed loads kraft paper bag, is placed in 4 DEG C of refrigerators and preserves.
In the step 2, alcohol is 70% alcohol, is soaked 30 seconds；The mass concentration of liquor potassic permanganate be 0.1~
0.3%, disinfecting time is 15~20 minutes；With aseptic water washing 4~5 times.
In the step 2, when cutting kind skin with aseptic operation knife, embryo position need to be avoided.
In the step 2, a diameter of 15 centimetres of culture dish, 50, seed is placed per ware.
In the step 3,60~80 milliliters of seed treatment, soaking at room temperature -3 days 2 days are poured into each culture dish.
In the step 3, seed treatment composition is：Gibberellin 50-100mg/L, 6-benzyladenine 0.05-0.1mg/
L, potassium dihydrogen phosphate 170-340mg/L, surplus are deionized water.
In the step 4, the size of sand basin is：It is long 5 meters, wide 90 centimetres, deep 20 centimetres；Filling is to a height of 15 lis in sand basin
The clean river sand of rice；Thickness 1-3cm river sands are spread on seed face and cover seed.
Beneficial effect：The present invention passes through physics and chemistry by choosing the ripe seed that grows in Chinese scholar tree original producton location
Method processing, the germination percentage of Chinese scholar tree seed is stable more than 99.5%, and this method can apply to the extensive nursery life of Chinese scholar tree
In production practice, there is the advantages that inexpensive, technology is simple and practical, and application value is high.Method provided by the invention, first by disappearing
Poison removes Chinese scholar tree seed top layer miscellaneous bacteria, afterwards cuts kind of a skin using sharp scalpel, is then soaked again with seed treatment,
The germination percentage and germinating energy of Chinese scholar tree seed can be significantly improved by being broadcast finally by sand, and it is rapid, neat to emerge, and growing way is consistent.
Embodiment
The present invention is realized by following steps：Seed selection, seed disinfection processing, cutting kind skin, seed treatment immersion, river
Sand sowing, wherein, seed treatment is by gibberellin (GA3), 6-benzyladenine (6-BA), potassium dihydrogen phosphate and deionization
Water forms.The method of the present invention specifically includes following steps：
1st, material：Chinese scholar tree (Sophora japonica) seed.
2nd, seed is gathered：It is strong that robust growth, strong stress resistance, disease and insect resistance are chosen from In Nanjing Chinese scholar tree plant cultivation base
Chinese scholar tree strain as seed collecting plant, with the ripening fruits of hand getting collection Chinese scholar tree, be fitted into ventilative good seed collecting bag, dry in the air naturally
Dry, Fruit pod is gone in stranding, after exposure seed 16-24 hours in the sun, the storage of seeds moisture is controlled in 9%-12%, full
Seed loads kraft paper bag, is placed in 4 DEG C of refrigerators and preserves.
3rd, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, soaked in 70% alcohol
30 seconds, then with the disinfecting solution of potassium permanganate 15~20 minutes that mass concentration is 0.1~0.3%, aseptic water washing 4~5 times, in
Blot the moisture of the surface of the seed on superclean bench under aseptic condition with aseptic filter paper, then cut kind of a skin one with aseptic operation knife
Individual osculum, need to avoid embryo position, and a diameter of 15 centimetres of culture dish, kind is placed per ware to sprouting in culture dish for uniform broadcasting
Son 50.
4th, seed treatment soaks：60~80 milliliters of seed treatment, room are poured into each culture dish for being covered with seed
Warm (25 ± 2) DEG C immersion -3 days 2 days, obtains the Chinese scholar tree seed of imbibition.Seed treatment forms：Gibberellin (GA3)50-
100mg/L, 6-benzyladenine (6-BA) 0.05-0.1mg/L, potassium dihydrogen phosphate 170-340mg/L, surplus is deionized water.
5th, the husky seed for broadcasting imbibition：It is that long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in 90% cool canopy in shading rate
Pond, sand basin are filled to a height of 15 centimetres of clean river sand, 20-25% water content, uniform broadcasting seed on sand basin face, then in
Thickness 1-3cm river sands are spread on seed face and cover seed.Germination percentage is counted after 7d, the Chinese scholar tree percentage of seedgermination handled through this method
Up to more than 99.5%.
6th, upper cup is heeled in：Using 2.7 cun of white plastic cups, matrix is red soil, high when Chinese scholar tree grows to two panels true leaf
At 10-15 centimetres, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
According to following embodiments, the present invention can be better understood from.It is however, as it will be easily appreciated by one skilled in the art that real
Apply specific material proportion, process conditions and its result described by example and be merely to illustrate the present invention, without that will not also should limit
The present invention described in detail in claims processed.
Embodiment 1
1st, seed is gathered：It is strong that robust growth, strong stress resistance, disease and insect resistance are chosen from In Nanjing Chinese scholar tree plant cultivation base
Chinese scholar tree strain as seed collecting plant, with the ripening fruits of hand getting collection Chinese scholar tree, be fitted into ventilative good seed collecting bag, dry in the air naturally
Dry, Fruit pod is gone in stranding, after exposure seed 16 hours, the storage of seeds moisture control in 12%, full seed are loaded in the sun
Kraft paper bag, it is placed in 4 DEG C of refrigerators and preserves.
2nd, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, soaked in 70% alcohol
30 seconds, then with the disinfecting solution of potassium permanganate 20 minutes that mass concentration is 0.1~0.3%, aseptic water washing 5 times, in ultra-clean work
Make to be blotted the moisture of the surface of the seed with aseptic filter paper under aseptic condition on platform, it is one small then to cut kind of skin with aseptic operation knife
Mouthful, embryo position need to be avoided, a diameter of 15 centimetres of culture dish, seed 50 is placed per ware to sprouting in culture dish for uniform broadcasting
Grain.
3rd, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed：60 milliliters, room temperature
(25 ± 2) DEG C immersion 3 days, obtains the Chinese scholar tree seed of imbibition.Seed treatment composition for GA3 50mg/L, 6-BA 0.05mg/L,
Potassium dihydrogen phosphate 170mg/L, surplus are deionized water.
4th, the husky seed for broadcasting imbibition：It is that long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in 90% cool canopy in shading rate
Pond, sand basin are filled to a height of 15 centimetres of clean river sand, 25% water content, and uniform broadcasting seed is on sand basin face, then in kind
Thickness 2cm river sands are spread on sub- face and cover seed.Germination percentage is counted after 7d, the Chinese scholar tree percentage of seedgermination handled through this method is up to
More than 99.5%.
5th, upper cup is heeled in：Using 2.7 cun of white plastic cups, matrix is red soil, high when Chinese scholar tree grows to two panels true leaf
At 10-15 centimetres, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
Embodiment 2
1st, seed is gathered：It is strong that robust growth, strong stress resistance, disease and insect resistance are chosen from In Nanjing Chinese scholar tree plant cultivation base
Chinese scholar tree strain as seed collecting plant, with the ripening fruits of hand getting collection Chinese scholar tree, be fitted into ventilative good seed collecting bag, dry in the air naturally
Dry, Fruit pod is gone in stranding, after exposure seed 24 hours, the storage of seeds moisture control in 10%, full seed are loaded in the sun
Kraft paper bag, it is placed in 4 DEG C of refrigerators and preserves.
2nd, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, soaked in 70% alcohol
30 seconds, then with the disinfecting solution of potassium permanganate 20 minutes that mass concentration is 0.15%, aseptic water washing 5 times, in superclean bench
The moisture of the surface of the seed is blotted under upper aseptic condition with aseptic filter paper, then cuts kind of one osculum of skin with aseptic operation knife, is needed
Embryo position is avoided, uniform broadcasting a diameter of 15 centimetres of culture dish, 50, seed is placed per ware to sprouting in culture dish.
3rd, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed：80 milliliters, room temperature
(25 ± 2) DEG C immersion 2 days, obtains the Chinese scholar tree seed of imbibition.Seed treatment composition is GA3 100mg/L、6-BA 0.1mg/L、
Potassium dihydrogen phosphate 340mg/L, surplus are deionized water.
4th, the husky seed for broadcasting imbibition：It is that long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in 90% cool canopy in shading rate
Pond, sand basin are filled to a height of 15 centimetres of clean river sand, 25% water content, and uniform broadcasting seed is on sand basin face, then in kind
Thickness 2cm river sands are spread on sub- face and cover seed.Germination percentage is counted after 7d, the Chinese scholar tree percentage of seedgermination handled through this method is up to
More than 99.8%.
5th, upper cup is heeled in：Using 2.7 cun of white plastic cups, matrix is red soil, high when Chinese scholar tree grows to two panels true leaf
At 10-15 centimetres, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
- A kind of 1. fast and effective method for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：Comprise the following steps：Step 1, seed is gathered：The seed for maturation of growing is chosen from Chinese scholar tree original producton location, is placed in preserving in 4 DEG C of refrigerators；Step 2, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, soaked in alcohol, then use Disinfecting solution of potassium permanganate, then with aseptic water washing, seed is blotted with aseptic filter paper under aseptic condition on superclean bench The moisture on surface, kind of one osculum of skin then is cut with aseptic operation knife, uniform broadcasting is into sprouting culture dish；Step 3, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed, soaking at room temperature, is obtained The Chinese scholar tree seed of imbibition；Step 4, the husky seed for broadcasting imbibition：It is that sand basin is established in 90% cool canopy in shading rate, sand basin fills clean river sand, river sand Water content is 20-25%, and uniform broadcasting seed then covers seed on sand basin face in layer overlay river sand on seed face；Step 5, upper cup is heeled in：Using plastic cup, matrix is red soil, high 10-15 centimetres when Chinese scholar tree grows to two panels true leaf When, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
- 2. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 1, the method for choosing the seed for maturation of growing is：Robust growth is chosen from In Nanjing Chinese scholar tree plant cultivation base, is resisted The inverse Chinese scholar tree strain that property is strong, disease and insect resistance is strong is as seed collecting plant, with the ripening fruits of hand getting collection Chinese scholar tree.
- 3. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1 or 2, it is characterised in that：It is described In step 1, after selected seed, seed is handled as follows：Seed is fitted into ventilative seed collecting bag, naturally dry, stranding is gone Fruit pod, after exposure seed 6-24 hours in the sun, the storage of seeds moisture controls in 9%-12%, full seed loaded Kraft paper bag, it is placed in 4 DEG C of refrigerators and preserves.
- 4. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 2, alcohol is 70% alcohol, is soaked 30 seconds；The mass concentration of liquor potassic permanganate be 0.1~0.3%, disinfecting time be 15~ 20 minutes；With aseptic water washing 4~5 times.
- 5. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 2, when cutting kind skin with aseptic operation knife, embryo position need to be avoided.
- 6. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 2, a diameter of 15 centimetres of culture dish, 50, seed is placed per ware.
- 7. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 3,60~80 milliliters of seed treatment, soaking at room temperature -3 days 2 days are poured into each culture dish.
- 8. the method for the fast and effective raising Chinese scholar tree percentage of seedgermination according to claim 1 or 7, it is characterised in that：It is described In step 3, seed treatment composition is：Gibberellin 50-100mg/L, 6-benzyladenine 0.05-0.1mg/L, potassium dihydrogen phosphate 170-340mg/L, surplus are deionized water.
- 9. the fast and effective method for improving Chinese scholar tree percentage of seedgermination according to claim 1, it is characterised in that：The step In 4, the size of sand basin is：It is long 5 meters, wide 90 centimetres, deep 20 centimetres；The clean river sand of filling to a height of 15 centimetres in sand basin；Kind Thickness 1-3cm river sands are spread on sub- face and cover seed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107567756A (en) * | 2017-09-29 | 2018-01-12 | 南京仙草堂生物科技有限公司 | A kind of method for improving Chinese scholar tree percentage of seedgermination |
| CN111066589A (en) * | 2019-12-25 | 2020-04-28 | 嘉兴市农业科学研究院 | Method for promoting rape late direct seeding, early growth and quick growth and application thereof |
| CN111937527A (en) * | 2019-11-13 | 2020-11-17 | 深圳职业技术学院 | Method for improving germination rate of sillago sophora seeds for sand fixation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472053A (en) * | 2014-11-07 | 2015-04-01 | 刘双发 | Germination processing method for sophora japonica seed |
| CA2995741A1 (en) * | 2015-09-11 | 2017-03-16 | Heliae Development, Llc | Microalgae based compositions and methods for application to plants |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
-
2017
- 2017-09-28 CN CN201710894293.6A patent/CN107683652A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472053A (en) * | 2014-11-07 | 2015-04-01 | 刘双发 | Germination processing method for sophora japonica seed |
| CA2995741A1 (en) * | 2015-09-11 | 2017-03-16 | Heliae Development, Llc | Microalgae based compositions and methods for application to plants |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107567756A (en) * | 2017-09-29 | 2018-01-12 | 南京仙草堂生物科技有限公司 | A kind of method for improving Chinese scholar tree percentage of seedgermination |
| CN111937527A (en) * | 2019-11-13 | 2020-11-17 | 深圳职业技术学院 | Method for improving germination rate of sillago sophora seeds for sand fixation |
| CN111066589A (en) * | 2019-12-25 | 2020-04-28 | 嘉兴市农业科学研究院 | Method for promoting rape late direct seeding, early growth and quick growth and application thereof |
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Application publication date: 20180213 |