CN105917794A - Seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance - Google Patents

Seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance Download PDF

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Publication number
CN105917794A
CN105917794A CN201610305670.3A CN201610305670A CN105917794A CN 105917794 A CN105917794 A CN 105917794A CN 201610305670 A CN201610305670 A CN 201610305670A CN 105917794 A CN105917794 A CN 105917794A
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China
Prior art keywords
seed
treatment
seeds
hance
rewetting
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CN201610305670.3A
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Chinese (zh)
Inventor
王磊
蒋泽平
邱国金
朱朝琴
王玉刚
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Suqian Institute of Agricultural Sciences of Jiangsu Academy of Agricultural Sciences
Jiangsu Forestry Academy
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Suqian Institute of Agricultural Sciences of Jiangsu Academy of Agricultural Sciences
Jiangsu Forestry Academy
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Priority to CN201610305670.3A priority Critical patent/CN105917794A/en
Publication of CN105917794A publication Critical patent/CN105917794A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to a seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance. The method mainly includes the steps of seed collecting, seed purifying, plant extract treatment, hormone treatment, ultra-drying treatment, storage, ultrasonic treatment and rewetting treatment. In the step of hormone treatment, seeds are soaked with gibberellin and auxin mixed liquor for 24 h; in the step of ultra-drying treatment, a silica gel drying method is adopted, the weight ratio of silica gel to the seeds is 5:1, used silica gel is replaced with fully-cooled dry silica gel every day, and the Dalbergia hupeana Hance seeds with the water content of 3.9-4% are obtained by controlling treatment time; in the step of storage, the seeds are sealed in a double-layer aluminum foil bag to be stored at the room temperature; in the step of ultrasonic treatment, the seeds at different grades are treated under different ultrasonic conditions; in the step of rewetting treatment, a step-by-step resetting method is adopted. By means of the method, the Dalbergia hupeana Hance seeds can show high vitality and resistance after being stored for a long term; compared with a conventional seed treatment method, the germination rate, germination potential, germination index and vitality index of the Dalbergia hupeana Hance seeds can be significantly increased.

Description

A kind of yellow wingceltis seeding and seedling raising method for treating seeds
Technical field
The present invention relates to yellow wingceltis nursery field, particularly relate to a kind of yellow wingceltis seeding and seedling raising method for treating seeds.
Background technology
Yellow wingceltis (formal name used at school: Dalbergia hupeana Hance), another name: do not know the spring, hope water wingceltis, Tan Shu, Sandalwood, Symplocoris Paniculatae;Papilionaceae, Dalbergia magaphanerophytes, is a kind of Landscape Application high-quality seeds;Wood quality Structure is fine and closely woven, is high-quality negative G and the strong apparatus of pulling force and equipment makes material.Root bark is pharmaceutically acceptable, has Heat-clearing and toxic substances removing, effect of hemostasia and detumescence.It is distributed widely in all over China.
The yellow wingceltis 3-4 month blooms, and pod is the most ripe October then, and the seed spring sowing time is the 2-3 month, because of The storage of this yellow wingceltis seed demand was sowed after 4-5 month, and after hiding the 4-5 month with conventional method sand, percentage of seedgermination is bright Aobvious reduction, before storage, percentage of seedgermination is about 70%, and after storage, germination percentage is down to 38%, and seed vitality refers to Number reduces;Another kind of more common plantation preservation condition is-18 DEG C of cryopreservations, it is possible to still protect after keeping storage Hold higher germination percentage, but the administration fee setting up low-temp bunker and maintenance low temperature is the highest, for list For individual nursery operating unit relatively difficult to achieve.
Summary of the invention
In order to solve above-mentioned technical problem, it is provided that one can be at room temperature storage and yellow wingceltis seed can be kept to send out Bud rate and vitality index are at the most high-caliber method for treating seeds, and the present invention provides techniques below scheme:
A kind of yellow wingceltis seeding and seedling raising method for treating seeds, comprises the following steps:
S1, seed collecting: October, when peel is become yellowish-brown from yellow green, win pod;Really pod is fully shone Do, rub, remove fruit pod.
S2, only kind: carry out empty flat seed, infected seed seed, the removing of damaged seed and field trash etc..
S3, plant extraction liquid process: seed is placed in the ginger extract of 5g/L immersion 12h;Described life Rhizoma Zingiberis Recens extracting solution use microwave-assisted leach extraction method, with distilled water as polar solvent, solid-liquid ratio as 1:6, microwave Power is 550W, microwave treatment time is 60s, and being diluted with water to concentration after having extracted is 5g/L;Swashing Element uses ginger extract to process before processing significant hormone absorption enhancement effect, it is possible to strengthen the life of hormone Thing availability, raising yellow wingceltis percentage of seedgermination, shortening hormone seed soaking time;
S4, HORMONE TREATMENT: first preparation HORMONE TREATMENT liquid, described HORMONE TREATMENT liquid includes the growth of 0.4g/L Element and the gibberellins of 0.6g/L, described auxin is heteroauxing, and seed is placed in HORMONE TREATMENT liquid leaching Bubble 24h;Use hormone seed soaking more notable by HORMONE TREATMENT effect again than after storage before storage, it is possible to be effectively improved Germination percentage, germinating energy and vitality index during later stage sowing.
S5, ultra-dry treatment: use silica dehydrator method, seed is placed in nylon net bag, is embedded in silicon in exsiccator In glue, silica gel and seed weight than for 5:1, at room temperature (daytime 25 DEG C, night 15 DEG C) dehydrate, Every day, timing was changed through 120 DEG C of sufficiently cool dry silica gels, weighs at regular intervals, is processed by control Time obtains the yellow wingceltis seed that water content is 3.94%;After HORMONE TREATMENT, the equilibrium water conten of seed rises relatively Height, now the ultra-dry treatment optimum moisture content of yellow wingceltis seed is 3.94%, after storage, compares other aqueous Amount, the seed of this water content and other each water content seeds relatively preserve before germination percentage, germination index and vigor The range of decrease of index is minimum.
S6, storage: seed is sealed in the room temperature preservation 4-5 month in double-layer aluminum-foil bag.
S7, supersound process: during to sowing time, first carry out seed grading, with seed length for classification foundation, Length more than 12mm for first order seed, length at 10-12mm for secondary seed, length is less than 10mm For three grades of seeds;Processing with ultrasound wave, described first order seed uses the ultrasonic Treatment of 50KHz again 12min, described secondary seed use ultrasonic Treatment 8min of 40KHz, described three grades of seeds to use 25KHz Ultrasonic Treatment 10min;Supersound process can improve soluble sugar content and the content of starch of yellow wingceltis seed, The water penetration of seed cell film can also be increased simultaneously, be conducive to improving effect, the shortening that next step rewetting processes Rewetting processes the time;Seed grading is placed under different ultrasound conditions carry out process can be to a certain degree On overcome the germination speed of seeding growing seedlings method to differ, uneven shortcoming so that seedling is more neat.
S8, rewetting process: seed is placed in saturated CaCl2In the exsiccator of aqueous solution (relative humidity 38%) Room temperature rewetting processes 8h, then moves to fill in the exsiccator of saturated NH4Cl aqueous solution (relative humidity 79%) Rewetting processes 8h, finally moves to fill in the exsiccator of distilled water, 40 DEG C of constant temperature wet process 16h next time.Return Wet process is sprouted the reparation of the Antioxidant Enzyme Systems of the ultra-dried seed effect of increasing significantly, beneficially seed Sending out, using rewetting process step by step is in order to avoid there is imbibition phenomenon, has the vitality restoration of ultra-dried seed Significantly facilitation, and owing to the supersound process of previous step makes the process time of this step significantly shorten.
Further, to stir seed during described step S4 HORMONE TREATMENT up and down to guarantee to be impregnated with uniformly.
Further, sow, if sowing not in time, then in 12h after described step S8 rewetting processes Seed is exposed under room temperature aging and deteriorating easily occur, is unfavorable for seed germination for a long time.
The beneficial effects of the present invention is:
(1), before ultra-dry treatment, use the auxin mixed treating liquid with gibberellins to yellow wingceltis seed at Reason, it is possible to significantly improve percentage of seedgermination after storage.
(2), exploration draws the super dry water content of the yellow wingceltis seed after HORMONE TREATMENT, by storage pair The adverse effect of seed germination is preferably minimized.
(3), the seed of optimum moisture content after processing with rewetting step by step through supersound process, index of respectively germinateing Exceed initial value, germination percentage, germination index and vitality index respectively than storage before improve 19.25%, 22.30%, 18.94%, germination percentage reaches 89.25%.
(4), the method for the present invention is used to carry out seeding and seedling raising seed treatment, it is possible to make after planting to emerge whole Together.
Detailed description of the invention
Embodiment 1,
A kind of yellow wingceltis seeding and seedling raising method for treating seeds, comprises the following steps:
S1, seed collecting: October, peel from yellow green become yellowish-brown time, win pod;Really pod fully dry, Rub, remove fruit pod.
S2, only kind: carry out empty flat seed, infected seed seed, the removing of damaged seed and field trash etc..
S3, plant extraction liquid process: seed is placed in the ginger extract of 5g/L immersion 12h;Described life Rhizoma Zingiberis Recens extracting solution use microwave-assisted leach extraction method, with distilled water as polar solvent, solid-liquid ratio as 1:6, microwave Power is 550W, microwave treatment time is 60s, and being diluted with water to concentration after having extracted is 5g/L.
S4, HORMONE TREATMENT: first preparation HORMONE TREATMENT liquid, described HORMONE TREATMENT liquid includes the growth of 0.4g/L Element and the gibberellins of 0.6g/L, described auxin is heteroauxing, and seed is placed in HORMONE TREATMENT liquid leaching Bubble 24h;During to stir seed up and down and guarantee to be impregnated with uniformly;
S5, ultra-dry treatment: use silica dehydrator method, seed is placed in nylon net bag, is embedded in silicon in exsiccator In glue, silica gel and seed weight than for 5:1, at room temperature (daytime 25 DEG C, night 15 DEG C) dehydrate, Every day, timing was changed through 120 DEG C of sufficiently cool dry silica gels, weighs at regular intervals, is processed by control Time obtains the yellow wingceltis seed that water content is 3.94%.
S6, storage: seed is sealed in the room temperature preservation 4-5 month in double-layer aluminum-foil bag.
S7, supersound process: during to sowing time, first with 50KHz ultrasonic Treatment seed 12min.
S8, rewetting process: seed is placed in saturated CaCl2In the exsiccator of aqueous solution (relative humidity 38%) Room temperature rewetting processes 8h, then moves to fill in the exsiccator of saturated NH4Cl aqueous solution (relative humidity 79%) Rewetting processes 8h, finally moves to fill in the exsiccator of distilled water, 40 DEG C of constant temperature wet process 16h next time.Return Sow in 1h after wet process.
Embodiment 2,
A kind of yellow wingceltis seeding and seedling raising method for treating seeds, comprises the following steps:
S1, seed collecting: October, peel from yellow green become yellowish-brown time, win pod;Really pod fully dry, Rub, remove fruit pod.
S2, only kind: carry out empty flat seed, infected seed seed, the removing of damaged seed and field trash etc..
S3, plant extraction liquid process: seed is placed in the ginger extract of 5g/L immersion 12h;Described life Rhizoma Zingiberis Recens extracting solution use microwave-assisted leach extraction method, with distilled water as polar solvent, solid-liquid ratio as 1:6, microwave Power is 550W, microwave treatment time is 60s, and being diluted with water to concentration after having extracted is 5g/L.
S4, HORMONE TREATMENT: first preparation HORMONE TREATMENT liquid, described HORMONE TREATMENT liquid includes the growth of 0.4g/L Element and the gibberellins of 0.6g/L, described auxin is heteroauxing, and seed is placed in HORMONE TREATMENT liquid leaching Bubble 24h;During to stir seed up and down and guarantee to be impregnated with uniformly;
S5, ultra-dry treatment: use silica dehydrator method, seed is placed in nylon net bag, is embedded in silicon in exsiccator In glue, silica gel and seed weight than for 5:1, at room temperature (daytime 25 DEG C, night 15 DEG C) dehydrate, Every day, timing was changed through 120 DEG C of sufficiently cool dry silica gels, weighs at regular intervals, is processed by control Time obtains the yellow wingceltis seed that water content is 3.94%.
S6, storage: seed is sealed in the room temperature preservation 4-5 month in double-layer aluminum-foil bag.
S7, supersound process: during to sowing time, first with 50KHz ultrasonic Treatment seed 12min.
S8, rewetting process: seed is placed in saturated CaCl2In the exsiccator of aqueous solution (relative humidity 38%) Room temperature rewetting processes 8h, then moves to fill in the exsiccator of saturated NH4Cl aqueous solution (relative humidity 79%) Rewetting processes 8h, finally moves to fill in the exsiccator of distilled water, 40 DEG C of constant temperature wet process 16h next time.Return Sow after wet process 6h.
After two embodiments of the present invention process, each germination index is as shown in the table, in table, and matched group 1 For without storage and the yellow wingceltis germination index (water content 30%) of process;Matched group 2 dries in the air for using nature Yellow wingceltis germination index (water content 12.1%) after Sand culture May after dry mode;Matched group 3 is for saving Go the germination index of ultrasound treatment step.
In analysis table understand, through the present invention method process after, yellow wingceltis seed through storage 5 months after still Keep higher germination percentage, germination index, and seed vitality is strong, enzymatic activity is high;Rewetting is entered after processing at once Sowing after seed is placed a period of time is compared in row sowing, and germination percentage is higher, seed vitality is higher.
With above-mentioned foundation desirable embodiment of the present invention for enlightenment, by above-mentioned description, related work people Member can carry out various change and amendment completely in the range of without departing from this invention technological thought. The content that the technical scope of this invention is not limited in description, it is necessary to according to right Determine its technical scope.

Claims (3)

1. a yellow wingceltis seeding and seedling raising method for treating seeds, it is characterised in that comprise the following steps:
S1, seed collecting: October, when peel is become yellowish-brown from yellow green, win pod;Really pod fully dry, Rub, remove fruit pod;
S2, only kind: carry out empty flat seed, infected seed seed, the removing of damaged seed and field trash etc.;
S3, plant extraction liquid process: seed is placed in the ginger extract of 5g/L immersion 12h;Described Rhizoma Zingiberis Recens carries Take liquid use microwave-assisted leach extraction method, with distilled water as polar solvent, solid-liquid ratio as 1:6, microwave power Being 60s for 550W, microwave treatment time, being diluted with water to concentration after having extracted is 5g/L;
S4, HORMONE TREATMENT: first preparation HORMONE TREATMENT liquid, described HORMONE TREATMENT liquid include the auxin of 0.4g/L with And the gibberellins of 0.6g/L, described auxin is heteroauxing, and seed is placed in HORMONE TREATMENT liquid immersion 24h;
S5, ultra-dry treatment: use silica dehydrator method, seed is placed in nylon net bag, be embedded in exsiccator in silica gel, Silica gel and seed weight are than for 5:1, at room temperature (daytime 25 DEG C, night 15 DEG C) dehydrate, every day Timing is changed through 120 DEG C of sufficiently cool dry silica gels, weighs at regular intervals, processes the time by control Obtain the yellow wingceltis seed that water content is 3.94%;
S6, storage: seed is sealed in the room temperature preservation 4-5 month in double-layer aluminum-foil bag;
S7, supersound process: during to sowing time, first carry out seed grading, with seed length for classification foundation, length More than 12mm for first order seed, length at 10-12mm for secondary seed, length less than 10mm's is Three grades of seeds;Process with ultrasound wave again, described first order seed use 50KHz ultrasonic Treatment 12min, Described secondary seed uses ultrasonic Treatment 8min of 40KHz, described three grades of seeds to use, and 25KHz's is ultrasonic Ripple processes 10min;
S8, rewetting process: seed is placed in saturated CaCl2(relative humidity 38%) room temperature in the exsiccator of aqueous solution Rewetting processes 1d, then moves to fill (relative humidity 79%) in the exsiccator of saturated NH4Cl aqueous solution and returns Wet process 1d, finally moves to fill in the exsiccator of distilled water, 40 DEG C of constant temperature wet process 2d next time.
2. a kind of yellow wingceltis seeding and seedling raising method for treating seeds as claimed in claim 1, it is characterised in that: institute To stir seed during stating step S4 HORMONE TREATMENT up and down to guarantee to be impregnated with uniformly.
3. a kind of yellow wingceltis seeding and seedling raising method for treating seeds as claimed in claim 1, it is characterised in that: institute State and sow in 12h after step S7 rewetting processes.
CN201610305670.3A 2016-05-10 2016-05-10 Seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance Pending CN105917794A (en)

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Publication number Priority date Publication date Assignee Title
CN106472072A (en) * 2016-09-28 2017-03-08 合肥沁牧生态农业有限公司 A kind of seeding growing seedlings method of kaffir lily
CN106717266A (en) * 2016-12-09 2017-05-31 浙江大学 The long-term preservation method of dendrobium candidum capsule/seed
CN109168415A (en) * 2018-10-31 2019-01-11 海南大学 A method of improving dalbergia odorifera percentage of seedgermination

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CN104488400A (en) * 2014-11-28 2015-04-08 广西壮族自治区药用植物园 Method for promoting germination of Yulangsan seeds

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472072A (en) * 2016-09-28 2017-03-08 合肥沁牧生态农业有限公司 A kind of seeding growing seedlings method of kaffir lily
CN106717266A (en) * 2016-12-09 2017-05-31 浙江大学 The long-term preservation method of dendrobium candidum capsule/seed
CN109168415A (en) * 2018-10-31 2019-01-11 海南大学 A method of improving dalbergia odorifera percentage of seedgermination

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Application publication date: 20160907