CN105815188A - Mid-late maturity peach seed direct germinating and seedling culturing technology - Google Patents
Mid-late maturity peach seed direct germinating and seedling culturing technology Download PDFInfo
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- CN105815188A CN105815188A CN201610366394.1A CN201610366394A CN105815188A CN 105815188 A CN105815188 A CN 105815188A CN 201610366394 A CN201610366394 A CN 201610366394A CN 105815188 A CN105815188 A CN 105815188A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a mid-late maturity peach seed direct germinating and seedling culturing technology. The technology includes the steps of peach seed stripping, surface disinfecting, inner seed peel stripping, germinating promoting, sowing and culturing and the like. By combining the key steps of inner seed peel stripping and germinating promoting, the seed germinating rate can be greatly increased. Peach seedlings can be obtained within 10-15 days; compared with a traditional technology, the obtaining time from seeds to peach seedlings is remarkably shortened, and the germinating rate of seeds being 75-95% can be achieved.
Description
Technical field
The present invention relates to the breeding technique of plant, a kind of Mid-late ripening peach seeds directly germinates seedling-raising technique specifically.
Background technology
Fructus Persicae is kernel approaches deciduous fruit tree, the seed of Mid-late ripening Fructus Persicae generally after cold stratification could breaking dormancy and sprout.In production, the time of peach seeds Stratificated treatment is 2～4 months；From mature fruit to obtaining Fructus Persicae seedling, short, need more than 60 days, long then more than half a year.If releasing seed dormancy early, it is possible to nursery ahead of time, for breeding, just can shorten breeding cycle.For the Mid-late ripening Fructus Persicae that July～October are ripe, sow after using Stratificated treatment breaking dormancy, happen mercury dropped or winter low temperature, need the normal growth by lagging facility guarantee Fructus Persicae Seedling.If able to shorten the time from seed to seedling, it is possible not only to save freezer facility and lagging facility operating cost, but also can to obtain seedling investigate early its phenotypic character or sampling carry out molecular marker screening work, more can reach certain trunk diameter period in next year spring grafting be used as the scion of grafting by accelerating Fructus Persicae Seedling fast-growth.
Summary of the invention
The invention discloses a kind of Mid-late ripening peach seeds directly to germinate seedling-raising technique, it can make seed without the direct germinating to seedling of cold stratification, greatly shorten from seed to the time obtaining Fructus Persicae seedling.
The present invention realizes above-mentioned purpose by the following technical solutions:
A kind of Mid-late ripening peach seeds directly germinates seedling-raising technique, and it comprises the following steps:
1) peach seeds is peeled off
Directly open the Peach fruits of maturation with nutcracker, take out seed and be put in the tissue culture bottle filling tap water, clean seed with tap water and be positioned in triangular flask；
2) surface sterilization
Seed is immersed 0.5%H2O2In solution, shaking table low speed slowly shakes 4h；
3) endotesta is divested
Peelling off seed coat on super-clean bench, kernel is put in clean glass beaker stand-by；
4) stratification processes
Seed half is immersed containing 0.2～0.5g/L gibberellins (GA3), 0.8～1.5g/L6-benzyl aminoadenine (6-BA) solution in process 24h；Optimize and be processed as in seed half immersion 0.3～0.4g/L gibberellins, 1.0～1.2g/L6-BA solution processing 24h；
5) sowing is cultivated
The seed that step 4) processes directly is sowed equipped with in the seedling culture hole plate of culture matrix, is placed in 22～28 DEG C of germinations, emerges, irregularly spray water with aerosol apparatus during germination, to keep plug media to moisten.
The culture matrix of described step 5 is black wood charcoal soil, peat, Nutrition Soil etc..
Described step 5 can substitute by following steps: by evenly laid out for the seed of different disposal in the culture dish containing moistening filter paper, put the group training indoor cultivation of long-day, temperature controls at 22～28 DEG C, cultivates until germination relaying after growing radicle in seedling culture hole plate.
The present invention uses technique scheme to have the advantages that
(1) peach seeds of mature fruit is not required to through the long-time Stratificated treatment of low temperature, can direct germinating to seedling, it is to avoid during long-term lamination, contingent seed is dead by microbial infection or the improper seed dormancy caused of lamination water content of substrate releases not exclusively or anaerobic respiration is dead；
(2) Fructus Persicae seedling can be obtained in the short period of time, draw materials for scientific research, in breeding seedling stage carry out Molecular Identification, shorten breeding cycle and provide convenience condition；
(3) cryogenic conditions of Stratificated treatment is eliminated the reliance on, it is also possible to before low temperature arrives, carry out germination work by natural light, temperature condition, save the energy, facility etc..
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1: " big Kubo " peach seeds directly germinates breeding experiment
The most ripe " big Kubo " Peach fruits, directly opens Peach fruits with nutcracker, takes out seed and is put in the tissue culture bottle filling tap water.Clean seed with tap water and be positioned in triangular flask.Seed is immersed 0.5%H2O2In solution, shaking table low speed slowly shakes 4h；Peelling off seed coat (endotesta) on super-clean bench, kernel is put in clean glass beaker stand-by.Kernel half is immersed room temperature treatment 24h in 0.3g/L gibberellins, 1.0g/L6-BA solution.Then the seed that processes is evenly laid out in the culture dish containing moistening filter paper, group training room (22～28 DEG C) putting the long-day is cultivated, and relays in the seedling culture hole plate equipped with culture matrix (black wood charcoal soil) until germination and after growing radicle；Emerge period irregularly water spray to keep culture matrix to moisten.It addition, comparison distilled water, 0.3g/L Gibberellins solution, 1.0g/L6-BA solution replace the mixed solution of above-mentioned gibberellins and 6-BA.Each process 20 seeds, 3 repetitions.Add up the germination percentage after sowing 10 days.
Result is as follows:
| Process | Average germination percentage (mean+SD %) |
| Peel off endotesta+distilled water to process | 34±11 |
| Peel off endotesta+Gibberellins solution to process | 73±9 |
| Peel off endotesta+6-BA solution to process | 53±6 |
| Peel off endotesta+gibberellins to process with 6-BA mixed solution | 87±11 |
The above results shows, after peeling off seed coat, 0.3g/L Gibberellins solution, 1.0g/L6-BA solution process relatively comparison distilled water and compares, and can improve the germination percentage of peach seeds, and wherein Gibberellins solution treatment effect becomes apparent from；Using 0.3g/L gibberellins, 1.0g/L6-BA solution to process relatively comparison phase comparison germination percentage and improve more, show the cooperative effect of stratification, average germination percentage is up to 87%.
Embodiment 2: pimple peach seeds directly germinates breeding experiment
" pimple Fructus Persicae " Peach fruits that mid-September is commercially ripe, directly opens Peach fruits with nutcracker, takes out seed and is put in the tissue culture bottle filling tap water.Clean seed with tap water and be positioned in triangular flask.Seed is immersed 0.5%H2O2In solution, shaking table low speed slowly shakes 4h；Peelling off seed coat (endotesta) on super-clean bench, kernel is put in clean glass beaker stand-by.Seed half is immersed room temperature treatment 24h in 0.4g/L gibberellins, 1.5g/L6-BA solution.The most directly sowing is in the seedling culture hole plate equipped with culture matrix (black wood charcoal soil), is placed in 22～28 DEG C of germinations, emerges；Emerge period irregular aerosol apparatus water spray to keep plug media to moisten.Peach seeds germination percentage after sowing 10 days is 92%, and Fructus Persicae growth of seedling is normal.
Claims (3)
1. a Mid-late ripening peach seeds directly germinates seedling-raising technique, it is characterised in that:
It comprises the following steps:
1) peach seeds is peeled off
Directly open the Peach fruits of maturation with nutcracker, take out seed and be put in the tissue culture bottle filling tap water, clean seed with tap water and be positioned in triangular flask；
2) surface sterilization
Seed is immersed 0.5%H2O2In solution, shaking table low speed slowly shakes 4h；
3) endotesta is divested
Peelling off endotesta on super-clean bench, kernel is put in clean glass beaker stand-by；
4) stratification processes
Kernel half is immersed containing 0.2～0.5g/L gibberellins (GA3), 0.8～1.5g/L6-benzyl aminoadenine (6-BA) solution in process 24h；
5) sowing is cultivated
The seed that step 4) processes directly is sown into equipped with in the seedling culture hole plate of culture matrix, is placed in 22～28 DEG C of germinations, emerges, irregularly spray water with aerosol apparatus during germination, to keep culture matrix to moisten.
A kind of Mid-late ripening peach seeds the most according to claim 1 directly germinates seedling-raising technique, it is characterised in that: divesting endotesta, kernel 0.3～0.4g/L gibberellins, 1.0～1.2g/L6-BA solution carry out stratification process 24h.
A kind of Mid-late ripening peach seeds the most according to claim 1 directly germinates seedling-raising technique, it is characterized in that: described step 5 following steps substitute: by evenly laid out for the seed of different disposal in the culture dish containing moistening filter paper, put the group training indoor cultivation of long-day, temperature controls at 22～28 DEG C, cultivates until germination relaying after growing radicle in seedling culture hole plate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610366394.1A CN105815188A (en) | 2016-05-30 | 2016-05-30 | Mid-late maturity peach seed direct germinating and seedling culturing technology |
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| CN201610366394.1A CN105815188A (en) | 2016-05-30 | 2016-05-30 | Mid-late maturity peach seed direct germinating and seedling culturing technology |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107820777A (en) * | 2017-11-14 | 2018-03-23 | 湖北省农业科学院果树茶叶研究所 | A kind of method for improving peach seeds germination rate |
| CN107969189A (en) * | 2017-12-15 | 2018-05-01 | 佛山市洛克威特科技有限公司 | A kind of method for improving peach percentage of seedgermination |
| CN109392583A (en) * | 2018-12-10 | 2019-03-01 | 江苏省农业科学院 | A method of promote peach embryo to train rosette-stape seedling seedling |
| BE1028953B1 (en) * | 2021-09-16 | 2022-07-19 | Shandong Inst Pomology | Methods for the rapid rearing of peach hybrid offspring |
Citations (3)
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| CN102835241A (en) * | 2012-09-18 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Method for raising peach tree seedlings in container |
| CN104969684A (en) * | 2014-04-11 | 2015-10-14 | 周凌云 | Method of quickly breaking dormancy of nectarine seeds |
| CN105284234A (en) * | 2015-09-26 | 2016-02-03 | 大连市农业科学研究院 | Method for increasing germination rate of sweet cherry seeds |
-
2016
- 2016-05-30 CN CN201610366394.1A patent/CN105815188A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835241A (en) * | 2012-09-18 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Method for raising peach tree seedlings in container |
| CN104969684A (en) * | 2014-04-11 | 2015-10-14 | 周凌云 | Method of quickly breaking dormancy of nectarine seeds |
| CN105284234A (en) * | 2015-09-26 | 2016-02-03 | 大连市农业科学研究院 | Method for increasing germination rate of sweet cherry seeds |
Non-Patent Citations (2)
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| 张义: "桃种子休眠原因及解除休眠方法研究概述", 《湖北农学院学报》 * |
| 苏明申等: "桃品种打破休眠的研究", 《上海农业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107820777A (en) * | 2017-11-14 | 2018-03-23 | 湖北省农业科学院果树茶叶研究所 | A kind of method for improving peach seeds germination rate |
| CN107820777B (en) * | 2017-11-14 | 2020-11-06 | 湖北省农业科学院果树茶叶研究所 | Method for improving germination rate of peach seeds |
| CN107969189A (en) * | 2017-12-15 | 2018-05-01 | 佛山市洛克威特科技有限公司 | A kind of method for improving peach percentage of seedgermination |
| CN109392583A (en) * | 2018-12-10 | 2019-03-01 | 江苏省农业科学院 | A method of promote peach embryo to train rosette-stape seedling seedling |
| BE1028953B1 (en) * | 2021-09-16 | 2022-07-19 | Shandong Inst Pomology | Methods for the rapid rearing of peach hybrid offspring |
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