CN109125189B

CN109125189B - Inclusion essence capable of instantly brightening skin color and preparation method thereof

Info

Publication number: CN109125189B
Application number: CN201811289876.7A
Authority: CN
Inventors: 范国刚; 李雄山
Original assignee: Guangdong Basonnia Biotechnology Co ltd
Current assignee: Guangdong Basonnia Biotechnology Co ltd
Priority date: 2018-10-31
Filing date: 2018-10-31
Publication date: 2021-11-02
Anticipated expiration: 2038-10-31
Also published as: CN109125189A

Abstract

The invention relates to the technical field of skin care products, in particular to inclusion essence capable of instantly brightening skin color and a preparation method thereof, wherein the essence comprises the following raw materials in parts by weight: 15-20 parts of microcrystalline cellulose, 100.1-6.0 parts of coenzyme Q, 10-15 parts of protein-phosphate ester, 0.01-1.5 parts of protein silk peptide, 1.0-1.5 parts of carbomer resin, 0.01-2.0 parts of yeast extract, 5-10 parts of rose essential oil, 0.1-1.0 part of hydroxypropyl methyl cellulose, 0.01-3.0 parts of whitening agent, 8-18 parts of humectant and 2-7 parts of antioxidant. The essence disclosed by the invention is strong in biocompatibility and free from damage to skin, and the lipid inclusion matrix particles are prepared from fat-soluble active substances by using a nano-encapsulation technology, so that the stability and the biological activity of the active substances can be effectively improved, the transdermal efficiency of the active substances is promoted, the irritation of the active substances can be effectively reduced, the bioavailability of a human body on the lipid inclusion matrix particles is improved, the activity of tyrosinase is effectively inhibited, the generation of pigmentation is prevented, epidermal cells can be repaired, the effects of whitening and moisturizing are achieved, and the skin is bright and white.

Description

Inclusion essence capable of instantly brightening skin color and preparation method thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to inclusion essence capable of instantly brightening skin color and a preparation method thereof.

Background

Along with the continuous improvement of the living substance level of the current society, the demand of people on beauty is higher and higher, more and more people pay more attention to the protection of skin while pursuing beauty, particularly the skin health problems of the exposed skin such as face whitening, moisture preservation, water locking and the like, and the good skin can not only improve the confidence of people, but also make people obtain more favor in social life. Skin aging is mainly a decrease in the function of the stratum corneum, a decrease in the thickness of the epidermis, a decrease in hyaluronic acid, damage to the epidermal basement membrane, activation of epidermal heparinase, a decrease in dermal collagen fibers, structural disorder of collagen fibers, a change in elastin, a decrease in the function of lymphatic recovery, and the like.

The existing skin care products aim at the whitening problem, the whitening auxiliary agent with strong chemical property is basically adopted in the whitening products, even heavy metals harmful to human skin such as hormone or lead mercury are added in the skin care products, although the products have the improvement effect on skin aging, cortical aging, darkening of color, dullness and the like caused by environmental change and industrial pollution, the products with poor biocompatibility can really and effectively achieve the whitening and anti-aging effect by being used in large quantity or for a long time, so that the skin is seriously damaged, the skin is accumulated with garbage, the self-protection and defense capability of the skin is greatly weakened, and the skin is easy to generate phenomena such as telangiectasia erythema, facial skin atrophy, pigmentation and the like, so that the normal ecological balance of the skin is damaged.

Disclosure of Invention

In order to overcome the defects and shortcomings in the prior art, the invention aims to provide the inclusion essence capable of immediately brightening the skin color, the essence is strong in biocompatibility, safe and free of damage to the skin, lipid inclusions are prepared from fat-soluble active substances by using a nano-encapsulation technology, the stability and the biological activity of the active substances can be effectively improved, the transdermal efficiency of the active substances can be effectively promoted, the irritation of the active substances can be effectively reduced, the absorptivity and the availability of the active substances to a human body can be improved, the activity of tyrosinase can be effectively inhibited, the pigmentation can be prevented, epidermal cells can be repaired, the whitening and moisturizing effects can be achieved, the skin can be brightened and white, and the skin color can be brightened.

The invention also aims to provide a preparation method of the inclusion essence capable of immediately brightening the skin color, the preparation method is easy to operate, avoids the damage of active substances caused by high-temperature sterilization in the prior art, is low in production cost, can be used for large-scale production, and the prepared inclusion essence can effectively inhibit tyrosinase activity, repair epidermal cells, remove skin free radicals, remarkably improve the effects of whitening and moisturizing, enable the skin to be bright and white and moisten, and brighten the skin color.

The purpose of the invention is realized by the following technical scheme: an inclusion essence capable of instantly brightening skin color comprises the following raw materials in parts by weight:

each part of the inclusion base particle comprises the following raw materials in parts by weight:

the essence is prepared by adopting the raw materials, and the microcrystalline cellulose can assist the combination of fat-soluble active substances (coenzyme Q10, protein silk peptide and yeast extract) to play a role in emulsifying and dispersing the coenzyme Q10, the protein silk peptide and the yeast extract, so that each active substance can be fully dispersed when an inclusion is prepared, and a better inclusion effect is achieved; the coenzyme Q10 plays a role of a fat-soluble antioxidant, can effectively activate human cells and nutrition of cell energy, turns into alcohol after entering the skin, and generates vitamin E through direct reaction with peroxide free radicals, so that the antioxidant, aging delaying and whitening effects of the essence are further enhanced; the protein-phosphate ester is biocompatible ester, and when the protein-phosphate ester is applied to the skin, the protein-phosphate ester carries active substances to smoothly pass through a cell phospholipid bilayer by utilizing the compatibility of the protein-phosphate ester and the skin, so that the cells are deeply repaired, the biological rejection is relieved, the permeation time of the essence is shortened, and the effect of immediately brightening the skin is achieved; the protein silk peptide as the fat-soluble active substance is a natural active substance, has strong permeability and no side effect on the skin, plays a moisturizing role for coating the skin, and can inhibit the activity of tyrosinase in the skin, thereby inhibiting the tyrosinase from generating melanin and brightening the skin color; the carbomer resin is a water-soluble emulsifier and can assist protein silk peptide to carry out fat-soluble coating, so that the absorption efficiency of the skin to the essence can be improved when the carbomer resin acts on the skin; the yeast extract is rich in micromolecular substances of natural active ingredients such as amino acid, peptide, nucleotide, vitamin and the like, wherein the amino acid enables the main ingredients of natural moisturizing factors in the horny layer of the skin to be easily absorbed by the skin, enables the aged epidermis to recover elasticity, delays skin aging, simultaneously can also enhance SOD activity, improves the anti-free radical capacity of the skin, inhibits the generation of melanin and brightens the skin color; the hydroxypropyl methyl cellulose can assist the protein silk peptide to lock water on the surface layer of the skin, and meanwhile, a fat-soluble inclusion is manufactured to disperse active matters; the rose essential oil has the effects of inhibiting the proliferation of stem cells, inhibiting tyrosinase from generating melanin, brightening skin color and obviously enhancing the activity of skin cells in cell renewal, can effectively activate the skin by combining the antioxidation of the rose essential oil, has a fragrant and elegant taste and can relieve emotion and depression by inhibiting uricase.

Preferably, the bulk density of the microcrystalline cellulose is 0.35 to 0.45g/cm³。

The invention is further defined by the microcrystalline cellulose employed having a bulk density of from 0.35 to 0.45g/cm³Microcrystalline cellulose in this bulk density range is most fluid when used for inclusion formation, and is capable of effectively and fluidically encapsulating water sensitive coenzyme Q10 and protein silk peptide.

Preferably, the particle size of the protein silk peptide is 1.0-1.6 mm.

The invention strictly controls the particle size of the protein silk peptide to be 1.0-1.6mm, can effectively control the particle size of the fat-soluble inclusion prepared by nano-encapsulation, further maximizes the specific surface of the prepared fat-soluble inclusion and improves the capacity of loading active substances.

Preferably, each part of the whitening agent comprises the following raw materials in parts by weight:

0.001-0.05 part of peptide nucleic acid Meifactor

0.01-2.0 parts of GEF repair factor

0.01-1.0 part of licorice extract.

The whitening agent disclosed by the invention can be complemented with a gene sequence fragment of tyrosinase in a skin melanin cell sequence by adopting the peptide nucleic acid whitening factor, and can be specifically combined with the tyrosinase to achieve the effect of inhibiting the generation of the tyrosinase and the melanin, further radically reduce the generation of the melanin, accelerate the metabolism and discharge of epidermal melanin, and achieve the quick and long-acting whitening effect, the dosage of the peptide nucleic acid whitening factor is strictly controlled within the range of 0.001-0.05 part during the addition, the effect of effectively inhibiting the tyrosinase cannot be achieved if the dosage is too low, and the expression of the peptide nucleic acid whitening factor is influenced by the combination of the surplus and enzymes except the tyrosinase if the dosage is too high; the GEF repairing factor can effectively stimulate tyrosine phosphorylation of an epidermal cell growth factor receptor, so that differentiation and proliferation speeds of epidermal cells are promoted, stem cells are continuously differentiated into new cells, the content of melanocytes and pigment cells in the skin is reduced, and the effects of repairing the skin, whitening and moisturizing are finally achieved; the Glycyrrhrizae radix extract can inhibit the activity of tyrosinase and dopachrome tautomerase (TRP-2) from root, inhibit the polymerization of 5, 6-dihydroxyindole (DHl), and prevent the formation of melanin, thereby whitening skin.

Preferably, each part of the humectant comprises the following raw materials in parts by weight:

6-14 parts of PCA natural moisturizing factor

10-15 parts of hyaluronic acid.

The moisturizing agent in the raw materials of the invention adopts hyaluronic acid, which can help skin absorb a large amount of moisture in air, keep elasticity and softness, help skin to recover normal oil-water balance, and improve dry and loose skin, and the PCA natural moisturizing factor can further assist hyaluronic acid to improve the moisture content of skin, and improve the water locking capacity of the essence.

Preferably, each part of the antioxidant comprises the following raw materials in parts by weight:

0.01-3 parts of dipotassium glycyrrhizinate

Formic acid 0.0001-0.5 part

0.02-0.1 part of kasong.

The antioxidant in the raw materials of the invention is prepared by using dipotassium glycyrrhizinate and fat-soluble active substances together, which can accelerate the absorption of skin to the dipotassium glycyrrhizinate and the fat-soluble active substances and can assist the whitening agent to enhance the whitening effect; the cason has a certain antiseptic effect on the essence of the invention due to the characteristics of low toxicity, wide antibacterial action range and good antiseptic effect.

Preferably, each part of the solution is prepared by mixing deionized water and 1, 3-propylene glycol in a weight ratio of 40-60: 5-15.

The solvent in the raw materials of the invention is a mixture consisting of deionized water and 1, 3-propylene glycol, wherein the 1, 3-propylene glycol is safe and mild, does not cause phenomena such as irritation and discomfort to skin, can fully dissolve other components used in the technology, has a certain moisturizing effect, and improves the moisturizing performance of the essence.

Preferably, each portion of the inclusion base particle is prepared by the following steps:

1) mixing the microcrystalline cellulose, the protein-phospholipin, the protein silk peptide, the carbomer resin and the hydroxypropyl methyl cellulose according to the weight parts, and heating and dissolving to obtain a solution A for later use;

2) weighing the coenzyme Q10, the protein silk peptide and the yeast extract according to the parts by weight, and uniformly mixing and stirring to obtain a solution B for later use;

3) dispersing the solution B obtained in the step 2) into the solution A obtained in the step 1), homogenizing under the pressure of 2-3MPa for 20-30min, and cooling to obtain inclusion base particles.

The inclusion matrix of the invention can fully utilize the fat-soluble inclusion to prepare active substances into the nano-level inclusion matrix by utilizing the nano-lipid technology by adopting the method, and the prepared inclusion matrix has better compatibility with lipid components of skin cells by utilizing the excellent fat-soluble characteristic thereof, can effectively bring the active substances into the cells to repair and proliferate the active substances, and improve the permeability of the components in the essence, and the inclusion matrix prepared by the technology is used in the brightening and whitening essence to ensure that the prepared inclusion essence wraps the active substances, can effectively improve the stability and the bioactivity of the active substances, effectively promote the transdermal efficiency of the active substances, can reduce the irritation of the active substances directly acting on human cells, improve the bioavailability of the human body to the active substances, and can effectively inhibit the activity of tyrosinase, preventing pigmentation, repairing epidermal cells, whitening skin, moisturizing skin, and improving skin color.

The other purpose of the invention is realized by the following technical scheme: a preparation method of inclusion essence capable of instantly brightening skin color comprises the following steps:

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at the stirring speed of 1000-1600rmp, uniformly stirring, and standing for 15-20min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and performing constant-temperature ultrasonic stirring at an ultrasonic frequency of 25000 and 30000Hz for 10-15min to obtain an inclusion essence primary solution;

3) sterilizing the primary inclusion essence obtained in the step 2) under the ultraviolet ray with the wavelength of 200-.

The preparation steps of the inclusion essence capable of instantly brightening the skin color are as described above, the operation steps are simple, the control is convenient, the production efficiency is high, the damage of active substances caused by traditional high-temperature sterilization is avoided, the production cost is low, and the inclusion essence can be used for large-scale production. The preparation method comprises the steps of strictly controlling the adding sequence of the raw materials, fully utilizing the synergistic effect among the raw materials, adding the rose essential oil, the whitening agent and the moisturizing agent into a solvent, improving the intersolubility of the rose essential oil, the whitening agent and the moisturizing agent, keeping the activity and the adaptability of the rose essential oil in the solvent by high-speed stirring, forming a core framework for whitening, moisturizing and repairing, enabling the whitening agent and the rose essential oil to permeate to the deep layer of skin to play a role in the permeation of the moisturizing agent and the solvent, then adding the antioxidant and the inclusion base particles, improving the combination intersolubility of the inclusion base particles and the C liquid, and enabling the inclusion base particles, the moisturizing agent and the whitening agent to permeate to the deep layer of skin to play a purifying and brightening role in the permeation of the solvent.

Wherein the rose essential oil, the whitening agent and the pack moisturizing agent can be well dispersed in the solvent by strict stirring speed; if the ultrasonic frequency in the step 2) is too low, the effect of sufficient mutual solubility cannot be achieved, and if the ultrasonic frequency is too high, the molecular structure of the peptide nucleic acid whitening factor and the inclusion matrix are easily damaged; the wavelength during sterilization is strictly controlled in the step 3), the wavelength is not short enough, if the wavelength is short enough, the molecular structures of the GEF repair factor and the peptide nucleic acid whitening factor are broken, and if the wavelength is too high, the sterilization effect cannot be achieved.

The invention has the beneficial effects that: the inclusion essence disclosed by the invention is strong in biocompatibility, high in safety and free of damage to skin, lipid-soluble active substances are prepared into lipid inclusion base particles by using a nano-encapsulation technology, and the active substances are encapsulated, so that the stability and the bioactivity of the active substances are effectively improved, the transdermal efficiency of the active substances is effectively promoted, the permeation time and the action time of the active substances are shortened, the skin color brightening efficiency of the active substances is improved, the irritation of the active substances directly acting on human body cells can be reduced, the bioavailability of the active substances by a human body is improved, the activity of tyrosinase is effectively inhibited, the pigmentation is prevented, epidermal cells can be repaired, the effects of whitening and moisturizing are achieved, the skin is bright and white, and the skin color is brightened.

The preparation method disclosed by the invention is simple to operate, avoids the damage of the traditional high-temperature sterilization mode to active substances, is low in production cost and high in production efficiency, can be used for large-scale production, and the prepared inclusion essence can effectively inhibit tyrosinase activity, repair epidermal cells, remove skin free radicals, remarkably improve the effects of whitening and moisturizing, enable the skin to be bright and white and moisten, and improve the skin color.

Detailed Description

The present invention will be further described with reference to the following examples for facilitating understanding of those skilled in the art, and the description of the embodiments is not intended to limit the present invention.

Example 1

An inclusion essence capable of instantly brightening skin color comprises the following raw materials in parts by weight:

wherein the microcrystalline cellulose has a bulk density of 0.35g/cm³The particle size of the protein silk peptide is 1.0 mm.

Each part of the whitening agent comprises the following raw materials in parts by weight:

peptide nucleic acid Meifactor 0.001 parts

0.01 portion of GEF repair factor

0.01 part of licorice extract.

Each part of the humectant comprises the following raw materials in parts by weight:

PCA natural moisturizing factor 6 parts

10 parts of hyaluronic acid.

Each part of the antioxidant comprises the following raw materials in parts by weight:

0.01 part of dipotassium glycyrrhizinate

Formic acid 0.0001 part

0.02 part of cason.

Each part of the solution is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 40: 5, and (c) a mixture of the components.

Each part of the inclusion base particle is prepared by the following steps:

3) dispersing the solution B obtained in the step 2) in the solution A obtained in the step 1), homogenizing for 20min under the pressure of 2MPa, and cooling to obtain inclusion base particles.

A preparation method of inclusion essence capable of instantly brightening skin color comprises the following steps:

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at the stirring speed of 1000rmp, uniformly stirring, and standing for 15min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and performing ultrasonic stirring at constant temperature, wherein the ultrasonic frequency is 25000Hz, and the ultrasonic time is 10min, so as to obtain an inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays with the wavelength of 200nm for 120min, and standing for 10min to obtain an inclusion essence finished product.

Example 2

wherein the microcrystalline cellulose has a bulk density of 0.375g/cm³The particle size of the protein silk peptide is 1.1 mm.

peptide nucleic acid Meifactor 0.01 parts

0.5 portion of GEF repair factor

0.25 part of licorice extract.

PCA natural moisturizing factor 8 parts

11 parts of hyaluronic acid.

1 part of dipotassium glycyrrhizinate

Formic acid 0.125 part

0.025 parts of kasong.

Each part of the solution is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 45: 7.

Each part of the inclusion base particle is prepared by the following steps:

3) dispersing the liquid B obtained in the step 2) in the liquid A obtained in the step 1), homogenizing under the pressure of 2.25MPa for 22.5min, and cooling to obtain inclusion base particles.

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at a stirring speed of 1150rmp, uniformly stirring, and standing for 16min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and carrying out constant-temperature ultrasonic stirring, wherein the ultrasonic frequency is 26000Hz, and the ultrasonic time is 12min, so as to obtain an inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays with the wavelength of 225nm for 125min, and standing for 13min to obtain an inclusion essence finished product.

Example 3

wherein the bulk density of the microcrystalline cellulose is 0.4g/cm³The particle size of the protein silk peptide is 1.3 mm.

peptide nucleic acid Meifactor 0.025 parts

GEF repair factor 1.0 part

0.5 part of liquorice extract.

10 parts of PCA natural moisturizing factor

12.5 parts of hyaluronic acid.

1.5 parts of dipotassium glycyrrhizinate

Formic acid 0.25 part

0.05 part of cason.

Each part of the solution is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 50: 10 to a mixture of the components.

Each part of the inclusion base particle is prepared by the following steps:

3) dispersing the solution B obtained in the step 2) in the solution A obtained in the step 1), homogenizing under the pressure of 2.5MPa for 25min, and cooling to obtain inclusion base particles.

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at the stirring speed of 1300rmp, uniformly stirring, and standing for 17min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and ultrasonically stirring at a constant temperature, wherein the ultrasonic frequency is 27000Hz, and the ultrasonic time is 13min to prepare an inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays with the wavelength of 250nm for 135min, and standing for 15min to obtain an inclusion essence finished product.

Example 4

wherein the microcrystalline fibersThe bulk density of the extract is 0.425g/cm³The particle size of the protein silk peptide is 1.4 mm.

peptide nucleic acid Meifactor 0.045 parts

GEF repair factor 1.5 parts

0.75 part of licorice extract.

PCA natural moisturizing factor 12 parts

13 parts of hyaluronic acid.

2 portions of dipotassium glycyrrhizinate

Formic acid 0.35 part

0.075 part of cason.

Each part of the solution is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 55: 12, respectively.

Each part of the inclusion base particle is prepared by the following steps:

3) dispersing the solution B obtained in the step 2) in the solution A obtained in the step 1), homogenizing for 27min under the pressure of 2.75MPa, and cooling to obtain inclusion base particles.

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at a stirring speed of 1450rmp, uniformly stirring, and standing for 18min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and carrying out constant-temperature ultrasonic stirring, wherein the ultrasonic frequency is 29000Hz, and the ultrasonic time is 14min, so as to obtain an inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays with the wavelength of 275nm for 145min, and standing for 15min to obtain an inclusion essence finished product.

Example 5

wherein the microcrystalline cellulose has a bulk density of 0.45g/cm³The particle size of the protein silk peptide is 1.6 mm.

peptide nucleic acid Meifactor 0.05 part

GEF repair factor 2.0 parts

1.0 part of licorice extract.

PCA natural moisturizing factor 14 parts

15 parts of hyaluronic acid.

3 portions of dipotassium glycyrrhizinate

Formic acid 0.5 part

0.1 part of cason.

Each part of the solution is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 60: 15, respectively.

Each part of the inclusion base particle is prepared by the following steps:

3) dispersing the solution B obtained in the step 2) in the solution A obtained in the step 1), homogenizing for 30min under the condition that the pressure is 3MPa, and cooling to obtain inclusion base particles.

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring at a stirring speed of 1600rmp, uniformly stirring, and standing for 20min to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and performing ultrasonic stirring at constant temperature, wherein the ultrasonic frequency is 30000Hz, and the ultrasonic time is 15min, so as to obtain an inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays with the wavelength of 300nm for 150min, and standing for 20min to obtain an inclusion essence finished product.

Comparative example 1

peptide nucleic acid Meifactor 0.001 parts

0.01 portion of GEF repair factor

0.01 part of licorice extract.

PCA natural moisturizing factor 6 parts

10 parts of hyaluronic acid.

0.01 part of dipotassium glycyrrhizinate

Formic acid 0.0001 part

0.02 part of cason.

Each part of the inclusion base particle is prepared by the following steps:

2) weighing the protein silk peptide and the yeast extract according to the weight parts, and uniformly mixing and stirring to obtain a solution B for later use;

Comparative example 2

peptide nucleic acid Meifactor 0.025 parts

GEF repair factor 1.0 part

0.5 part of liquorice extract.

10 parts of PCA natural moisturizing factor

12.5 parts of hyaluronic acid.

1.5 parts of dipotassium glycyrrhizinate

Formic acid 0.25 part

0.05 part of cason.

Each part of the inclusion base particle is prepared by the following steps:

2) weighing the coenzyme Q10 and the protein silk peptide according to the parts by weight, and uniformly mixing and stirring to obtain a solution B for later use;

Comparative example 3

peptide nucleic acid Meifactor 0.05 part

GEF repair factor 2.0 parts.

PCA natural moisturizing factor 14 parts

15 parts of hyaluronic acid.

3 portions of dipotassium glycyrrhizinate

Formic acid 0.5 part

0.1 part of cason.

Each part of the inclusion base particle is prepared by the following steps:

The essence containing the extracts prepared in specific examples 1 to 5 and the essence containing the extracts prepared in comparative examples 1 to 3 were respectively subjected to whitening, moisture absorption and retention efficacy, SOD activity and melanin value tests.

(I) detection method

1. Whitening effect

The whitening effect of the essence is evaluated by detecting the inhibition effect of the essence on the tyrosinase activity, and the lower the residual activity of the tyrosinase is, the better the inhibition effect of the essence on the tyrosinase activity is, and the better the whitening effect is.

The test is to measure the inhibition of tyrosinase and the activity of tyrosinase by the sample by ultraviolet spectrophotometry (analysis is performed by the selective absorption of light by substances). The experiment is carried out by using tyrosinase and dopa to form oxidation product dopachrome under the catalysis of enzyme in an ultraviolet region, wherein the dopachrome has maximum absorption at 475nm, and the absorption coefficient epsilon is 3700(cm & mol/L).

Using a hand-held pipette, 1.0mL of 2 mmoL/L-dopa and 2.6mL of a phosphate buffer (pH 6.8) were pipetted into cuvettes. The temperature was maintained in a constant-temperature water bath at 30 ℃ for 10min, and then 0.2mL of the essences (samples) of examples 1 to 5 and comparative examples 1 to 4 and 0.2mL of 100ug/mL of tyrosinase solution were added to constitute a 4mL solution system. The absorbance of the solution system was measured at a wavelength of 475nm as a function of time over 8 min. The tyrosinase residual activity (U) is calculated as follows:

U＝[(OD1-OD3)/OD2-OD4]×100％

in the formula, OD1 refers to the absorbance of a live test system containing a substrate, tyrosinase and a sample solution; OD3 represents the absorbance of the assay system containing only the substrate and the sample solution; OD2 is the absorbance of the activity detection system containing only substrate and tyrosinase solution; OD4 refers to the absorbance of the assay containing only the substrate solution.

2. Moisture absorption and retention effects

The moisture absorption and retention efficiency of the essence is evaluated through the moisture absorption rate and the moisture retention rate of the essence, and the higher the moisture absorption rate and the moisture retention rate is, the better the moisture absorption and retention effect is.

Will contain saturated (NH)₄)₂SO₄The inner chamber of the dryer containing the aqueous solution was set to have a relative humidity of 80% (RH ═ 80%), and the inner chamber of the dryer containing the saturated aqueous solution of K2SO4 was set to have a relative humidity of 40% (RH ═ 40%). 0.5g of the essences (samples) of examples 1 to 5 and comparative examples 1 to 3, which were dried to a constant weight, were accurately weighed and placed in small weighing bottles, respectively, in the above 2 dryers. Placing 2 dryers in a constant temperature environment with the temperature of 20 ℃, standing for 24 hours, weighing the mass of each sample, and calculating the moisture absorption rate:

moisture absorption rate (m2-m1)/m 1X 100%

m1 is the initial mass of the sample and m2 is the mass of the sample after 24 h.

0.5g of the essence solutions of examples 1 to 5 and comparative examples 1 to 4 dried to a constant weight were precisely weighed, deionized water having a sample weight of 10% was added, and the solution was placed in small weighing bottles and placed in the 2 dryers described above, respectively. Placing 2 dryers in a constant temperature environment with the temperature of 20 ℃, standing for 24 hours, weighing the mass of each sample, and calculating the moisture retention rate:

the moisture retention rate is m4/m3 x 100%

m3 is the initial moisture mass of the sample and m4 is the moisture mass of the sample after 24 h.

3. Determination of anti-aging Capacity

The essences prepared in examples 1 to 5 and the essences prepared in comparative examples 1 to 3 were subjected to anti-aging capacity measurement using SOD activity as an index.

50 Kunming clean-grade mice, half male and female, weight (25 +/-2) g, provided by the Experimental animal center of Guangdong university of medical science, were randomly divided into 5 groups (control group, sample group), and 10 mice each. The experimental interventions of the essences prepared in examples 1-5 of the present invention and in comparative examples 1-4 were performed daily by gavage of the sample groups starting from 8:30 in the first morning, and the mice were weighed 1 time every 1 day, and the gavage (10g/kg) was adjusted accordingly according to the body weight. The control group was also perfused with an equal amount of saline. After one month, the mouse cerebral cortex cells were taken to test the SOD activity.

4. Melanin value test

Randomly selecting 100 women in 20-45 years of age for voluntary test, arranging 10 experimenters in each example or comparative example (one example or the comparative example is tested in 10 groups), and carrying out skin melanin test on the same position of the cheek of each experimenter by using a German DJM skin tester to respectively test an initial value;

then, the extracts of examples 1 to 5 and comparative examples 1 to 3 were used for coating, the coating was carried out for 10 minutes, then the skin melanin value was measured at the same site, and the skin melanin value was measured after coating once a day for 1 day, 3 days, 7 days, 14 days, 21 days, and 28 days, respectively, and the average values were obtained from the data table.

(II) the result of the detection

The results of the test on the whitening and moisturizing efficacy and the SOD activity of the essences of examples 1 to 5 and comparative examples 1 to 3 are shown in table 1:

table 1 results of the serum whitening and moisturizing efficacies and SOD activity test in examples 1 to 5 and comparative examples 1 to 3

From the above results, it can be seen that the essences of examples 1 to 5 of the present invention all have good whitening and moisturizing effects, can effectively inhibit tyrosinase activity, and have good hygroscopicity and moisturizing properties. Among them, the essence in example 3 is the most excellent in whitening and moisturizing effects.

TABLE 2 test data table for melanin values of skin before and after 10min, 1 day, 3 days, 7 days, 14 days, 21 days, and 28 days after finishing with the essence

Examples	Before use	10min	1 day	3 days	7 days	14 days	21 days	28 days	Mean value of
										Example 1	25.01	24.85	24.85	24.78	24.70	24.61	24.54	24.45	24.68
Example 2	24.99	24.66	24.56	24.66	24.50	24.46	24.43	24.40	24.53
										Example 3	25.12	24.94	24.78	24.53	24.56	24.49	24.44	24.36	24.50
Example 4	25.03	24.75	24.76	24.69	24.64	24.53	24.50	24.47	24.52
										Example 5	24.89	24.63	24.88	24.78	24.61	24.43	24.45	24.42	24.6
Comparative example 1	24.88	24.80	24.90	24.89	24.80	24.81	24.78	24.76	24.82
										Comparative example 2	25.21	25.16	25.0	24.99	24.89	24.80	24.81	24.78	24.91
Comparative example 3	25.03	24.98	24.91	24.90	24.85	24.83	24.81	24.79	24.86

From the above results, it can be seen that the essences of examples 1 to 5 of the present invention all have a very good skin-lightening effect, and can effectively inhibit tyrosinase activity, thereby reducing melanin production, and the essence of example 3 has the best skin-lightening effect.

The anti-aging capability test shows that the essence prepared by the invention can obviously improve the SOD activity of the mouse cerebral cortex, further enhance the capability of an organism for removing free radicals, relieve the attack of the free radicals on macromolecules of a biological membrane, weaken the damage of excessive free radicals on the biological membrane, ensure the integrity of the structure and the function of the cell membrane, and delay the occurrence of apoptosis, thereby showing that the essence prepared by the invention has excellent anti-aging effect. The anti-free radical capability of the essence prepared in comparative examples 1-3 is obviously lower than that of the products prepared in examples 1-5, and further shows that the components of the product have a good synergistic interaction effect, so that the product can effectively remove free radicals of skin, effectively inhibit the activity of tyrosinase, inhibit the generation of pigmentation, repair epidermal cells, accelerate the renewal of collagen fibers and achieve the effects of resisting aging and tightening skin.

Compared with the example 1, the raw materials of the essence of the comparative example 1 are not added with coenzyme Q10, the anti-free radical ability, the moisture absorption and preservation efficacy, the anti-aging ability and the SOD activity of the essence prepared from the raw materials are detected, and the analysis shows that the anti-free radical ability, the moisture absorption and preservation efficacy, the anti-aging ability and the SOD activity of the essence are relatively reduced; the coenzyme Q10 added in the raw materials can effectively activate the nutrition of human cells and cell energy, and the vitamin E is changed into alcohol after entering the skin and is directly reacted with peroxide free radicals to generate vitamin E, so that the antioxidation, the aging delaying and the moisturizing rate of the essence are further enhanced, the effects of resisting aging and tightening the skin are achieved, and the skin is bright, white, smooth and elastic.

Compared with the example 3, the raw materials of the essence of the comparative example 2 are not added with the yeast extract, the essence prepared from the raw materials is subjected to free radical resistance, moisture absorption and moisture retention efficacy, anti-aging ability and SOD activity detection, and analysis shows that the free radical resistance, the moisture absorption and moisture retention efficacy, the anti-aging ability and the SOD activity of the essence are relatively reduced; the yeast extract is added into the raw materials, so that the aged epidermis can recover elasticity, the skin aging is delayed, the SOD activity can be enhanced, and the anti-free radical capability of the skin is improved.

Compared with the example 5, the liquorice extract is not added in the raw materials of the essence in the comparative example 3, the anti-free radical capacity, the moisture absorption and preservation efficacy, the anti-aging capacity and the SOD activity of the essence prepared from the raw materials are detected, and the analysis shows that the anti-free radical capacity, the moisture absorption and preservation efficacy, the anti-aging capacity and the SOD activity of the essence are relatively reduced; the raw materials are added with the licorice extract, so that the skin whitening cream has the effects of preventing the formation of melanin, promoting cell metabolism and promoting collagen hyperplasia, and further achieves the skin whitening effect.

The above-described embodiments are preferred implementations of the present invention, and the present invention may be implemented in other ways without departing from the spirit of the present invention.

Claims

1. An inclusion essence capable of instantly brightening skin color is characterized in that: the essence comprises the following raw materials in parts by weight:

the bulk density of the microcrystalline cellulose is 0.35-0.45g/cm³；

The particle size of the protein silk peptide is 1.0-1.6 mm;

6-14 parts of PCA natural moisturizing factor

10-15 parts of hyaluronic acid;

0.01-3 parts of dipotassium glycyrrhizinate

Formic acid 0.0001-0.5 part

0.02-0.1 part of kasong;

each part of the solvent is prepared from deionized water and 1, 3-propylene glycol in a weight ratio of 40-60: 5-15;

the inclusion base particle is prepared by the following steps:

2. A method for preparing the inclusion essence capable of instantly lightening skin color according to claim 1, wherein the method comprises the following steps: the method comprises the following steps:

1) sequentially adding the rose essential oil, the whitening agent and the humectant into the solvent according to the parts by weight, stirring, uniformly stirring, and standing to obtain a solution C for later use;

2) adding the antioxidant and the inclusion base particles into the solution C obtained in the step 1) while stirring, and ultrasonically stirring at a constant temperature to obtain inclusion essence primary solution;

3) sterilizing the inclusion essence primary liquid obtained in the step 2) under ultraviolet rays, and standing for 10-20min to obtain a finished inclusion essence.

3. The method for preparing inclusion essence capable of instantly brightening skin color according to claim 2, wherein the method comprises the following steps: in the step 1), the stirring speed is 1000-1600rmp, the stirring time is 12-18min, and the standing time is 15-20 min; in the step 2), the ultrasonic frequency is 25000-30000Hz, and the ultrasonic time is 10-15 min; in the step 3), the wavelength of the ultraviolet light is 200-300nm, and the sterilization time is 120-150 min.