CN1091149C - Method for cultivating Chinese caterpillar fungus - Google Patents
Method for cultivating Chinese caterpillar fungus Download PDFInfo
- Publication number
- CN1091149C CN1091149C CN93117581A CN93117581A CN1091149C CN 1091149 C CN1091149 C CN 1091149C CN 93117581 A CN93117581 A CN 93117581A CN 93117581 A CN93117581 A CN 93117581A CN 1091149 C CN1091149 C CN 1091149C
- Authority
- CN
- China
- Prior art keywords
- caterpillar fungus
- cans
- chinese caterpillar
- northeast
- zinc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a new strain of a Chinese northeast caterpillar fungus, a culture method thereof, and a Chinese northeast caterpillar fungus rich in zinc, germanium and selenium by using the strain. An extracting solution is prepared from the cultured Chinese northeast caterpillar fungus, and the extracting solution is prepared into a Tianwangbao nutriment with male silkworm moth, pallas pit viper, etc.
Description
The invention belongs to the processing of the cultivation of northeast Chinese caterpillar fungus (Cordyceps militaris (L.ex.Fr) Linkmandshurcum) DBC-888 bacterial strain and extracting solution thereof and prepare nourishing liquid, oral liquid field with this extracting solution.
Existing at present much is the nutritious prod that raw material is made with the edible fungus, eat oral liquid as the TAIYANGSHEN hedgehog hydnum, the nutritive medium (CN88103922) that edible mushrooms is made through the fermentation of saccharic acid enzyme process, cordyceps sonensis oral liquid (CN92109862.6), wherein CN92109862.6 is the active substance that extracts in the Cordyceps militaris, make oral liquid, but its used Cordyceps militaris productive rate is very low.Effect is also not satisfactory.
The northeast Chinese caterpillar fungus originates in the Northeast China, belongs to together with Cordyceps sinensis.
The inventor is to the existing several years of the research of northeast Chinese caterpillar fungus, and in the screening of superior strain, aspects such as the booth production of northeast Chinese caterpillar fungus have obtained breakthrough, and to the application of northeast Chinese caterpillar fungus, the preparation aspect of healthcare products also has one's own knack.
The object of the present invention is to provide the new bacterial strain of northeast Chinese caterpillar fungus of cultivation.
Another object of the present invention is to provide the method for cultivating described new bacterial strain.
It is that main raw material is equipped with the healthcare products such as the precious senior tonic solution of the king with anticancer health care effect that Chinese medicines such as male Bombycis mori, pallas pit viper are made that one of purpose of the present invention provides the rich selenium of cultivating out with special methods, rich germanium, rich zinc type northeast Chinese caterpillar fungus.
Another object of the present invention is to provide the method for the above-mentioned northeast of preparation Chinese caterpillar fungus health product.
Wild northeast Chinese caterpillar fungus originates in northeast, parasitizes on the larva or pupa of lepidopterous insects, grows one or more sporophores (stroma) summer and autumn.The sporophore head is bar-shaped, and is leaf, and longitudinal furrow is arranged, orange-yellow, for can pregnant part.Shank is thin slightly, and color is also lighter, and sporophore total length 15-80mm, diameter are 2-4mm, and sporophore top (last 2/3 place) has the perithecium of ellipse garden shape, and thecaspore is arranged in the shell, emits from capsule when ripe.
As everyone knows, organic zinc, germanium, selenium are having significant effect aspect anticancer, health care, the raising body immunity, and inorganic zinc, selenium, germanium can not be absorption of human body, even it is also very low perhaps to absorb its transformation efficiency.The inventor after deliberation after, turn out than wild northeast Chinese caterpillar fungus output height, and the content of nutrition also is higher than the new bacterial strain of wild northeast Chinese caterpillar fungus.
One .DBC-888 bacterial strain
New bacterial strain (Cordyceps militaris (L.ex.Fr) Link.mandshurcumDBC-888) is the preferred of process wild state, strain separating, rejuvenation, directive breeding are preferably come out, it grows a plurality of sporophores on silkworm chrysalis, it is bar-shaped that sporophore is, expand the upper end, for orange-yellow, the lower end is thin slightly, paler colour.The high 50-100mm of sporophore, diameter 3-5mm cultivates containing on the rice medium of special nutrition, each Cans growth 20-30 strain, the high 60-130mm of sporophore, diameter 5-9mm, whole sporophore is orange, and the base portion color is light slightly.3/5 place of sporophore begins to expand, and the head garden is pure, and is slightly sagging.
The characteristics of DBC-888 bacterial strain are:
1. output height, each Cans mean yield are 20-35 gram (general bacterial strain, every Cans mean yield are the 5-10 gram).
2. contamination resistance is strong, and (several ten thousand bottles every batch) pollution rate is 10-25% in scale operation, and the pollution rate of general bacterial strain is 30-50%.
3. adaptability is strong, and temperature resistant range is wide.Mycelia and sporophore are all not dead at 30 ℃ to-20 ℃.
4. variability is good: by changing the composition of substratum, add the trace element (zinc, germanium, selenium) of different concns in substratum, can get rich zinc, germanium, selenium type Chinese caterpillar fungus.And this rich zinc, germanium, selenium effect are not machinery absorption but a kind of plysiochemical variation, and promptly by mycelium, sporophore absorbs, transform and be enriched in the resin of cell, protein, youngster's fourth matter, in polysaccharide and the nucleic acid, be about to inorganic elements and become organism, make it to be easy to be absorption of human body.
The northeast Chinese caterpillar fungus DBC-888 that the present invention cultivates contains 18 seed amino acids, the trace element of 80 multiple beneficial (as zinc, selenium, germanium, manganese, iron, calcium, phosphorus, potassium etc.) cordycepin, N.F,USP MANNITOL, Cordyceps polysaccharide, SOD, multivitamin, oleic acid, linolic acid, carotene etc.
Two. the present invention cultivates the method for northeast Chinese caterpillar fungus DBC-888:
(1) seed culture
1. first class inoculum is cultivated.
Former bacterial classification: the inventor from Benxi, areas of Shenyang gathers wild northeast Chinese caterpillar fungus, and with its separation, preferred fixed kind and it.
The prescription of first class inoculum substratum (calculating by 100% weight): Succus Rhizoma Dioscoreae 10%, saltpetre 0.5%, potassium primary phosphate 0.8%, VITAMIN liquid 0.5-2%, ferrous sulfate 0.05%, sal epsom 0.6%, glucose 2%, zinc sulfate 0.01%, Nucleotide 1%, agar 2%, milk 0.5%, all the other are water.
Be loaded on the substratum of above-mentioned preparation in vitro that Intake Quantity accounts for 1/5 of test tube volume, tampon beyond the Great Wall is through autoclaving (1Kg/cm
2Pressure) after 30 minutes, take out and to put into the inclined-plane, under aseptic condition, the mycelia of scraping the DBC-888 first class inoculum that takes a morsel with transfering loop was cultivated about 4 days in 22 ℃ of thermostat containers on the inclined-plane, and mycelia is covered with the inclined-plane.The feature of this bacterial classification is: mycelia is pure white, and is sturdy dense, and growth is rapid, later stage secretion xanthein.
2. second-class liquid isolate: use reciprocating type shaking table, 90 times/minute, amplitude 75mm.
Substratum contains (by 100% weight): Fructus Hordei Germinatus juice 2%, potassium primary phosphate 1%, soil extract 2%, sal epsom 0.6%, zinc sulfate 0.01%, ferrous sulfate 0.03%, aminoacids complex (commercially available) 0.5%, all the other are water.
Above-mentioned substratum is loaded in the 500-1000ml triangular flask, covers 8 layers of gauze, the outside puts polyester plastics and tightens, and carries out autoclaving (1Kg/cm
2) 30 minutes, after the cooling, with the DBC-888 first class inoculum, under the aseptic condition, be inoculated in the triangular flask, 1 test tube first class inoculum connects 5-10 triangular flask, build bottle cap (removing above-mentioned plastics adventitia), be placed on shaking table (reciprocating type shaking table, 90 times/minute, amplitude 75mm) on, under 20 ℃ of conditions, cultivated 5 days, there are a large amount of white bacterium balls to form in the triangular flask, nutrient solution is limpid, is good secondary bacterium liquid.(using) for the rich zinc of cultivation, rich selenium, rich germanium northeast Chinese caterpillar fungus.
(2) cultivation of .DBC-888 Cordyceps sporophore:
1. the rich zinc type of artificial culture northeast Chinese caterpillar fungus: substratum contains: (by 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, millet swill 2%, animal blood 1%, white squash seed meal 0.1-1%, Herba Epimedii 0.01-0.05%, all the other are soya-bean milk.Above-mentioned prescription is called for short nutritive medium.Get rice 40-50 gram, add in the clean Cans of packing into, above-mentioned nutritive medium 35-55 gram.360-900 μ g zinc additive is soluble in water, be added in the Cans, do lid with polyester film, tighten.(1.2Kg/cm in pressure kettle
2) sterilized 30 minutes, when dropping to 20 ℃ of left and right sides, temperature inoculates.
Inoculation with cultivate: will grow good DBC-888 second-class liquid isolate, with special Glass tubing inoculator, absorption 10ml under aseptic condition, receives in the above-mentioned Cans, builds polyester film again and puts to 15-20 ℃ of culturing room and cultivate.Through covering with white hypha on the substratum at Cans about a week, on the white hypha surface flavous liquid secretion thing was arranged on the Cans wall in 15-20 days.Grow fruit body primordium about one month, generally will grow the sporophore of orange in 40-60 days.After the sporophore maturation, under aseptic condition, adopt down with tweezers.Build and put back to culturing room, can grow second batch again through about 20 days.To be 90-290 μ g/g (dry weight) improve 3-10 doubly than the content (30-80 μ g (dry weight)) of the Chinese caterpillar fungus zinc of common artificial culture to the zinc content of rich zinc Chinese caterpillar fungus.
2. the cultivation of rich selenium type northeast Chinese caterpillar fungus:
Substratum (nutritive medium) (by 100% weight).Potassium primary phosphate 1%, sal epsom 0.5-2%, VB1 0.5-2%, animal blood 1%, all the other are soya-bean milk.
Getting rice 35-40 gram, Radix Astragali 0.5-3 gram packs in the clean Cans, Ensure Liquid liquid 35-45 gram, the selenium additive of 360-900 μ g also is added in the Cans after water-soluble again, builds lid, autoclaving, inoculation, cultivation (40-50 days grow stroma), collecting method are with rich zinc Chinese caterpillar fungus.The selenium content of this Chinese caterpillar fungus is 3-200 μ g/g, than high several times to hundred times of the Chinese caterpillar fungus selenium content (0.02-1 μ g/g) (dry weight) of common artificial culture.
3. the cultivation of rich germanium type northeast Chinese caterpillar fungus:
Nutritive medium (calculating by 100% weight) potassium primary phosphate 1%, sal epsom 0.5%, VB1 0.5-2%, animal blood 1%, all the other are soya-bean milk.
Get rice 35-40 gram and add Ganoderma powder or join powder 0.2-1 gram in vain, Ensure Liquid liquid 35-50 restrains and gets in Cans in the water-soluble back adding of the germanium additive 360-900 μ g/g Cans again, builds lid.Autoclaving, inoculation, cultivation (40-60 days grow sporophore), collecting method are with rich zinc Chinese caterpillar fungus.The germanic amount of this rich germanium Chinese caterpillar fungus be 1-160 μ g/g (dry weight) than common artificial culture Chinese caterpillar fungus, germanic amount 0.015-2 μ g/g (dry weight) is high tens times.
4. on silkworm chrysalis, cultivate the method for rich zinc, rich germanium, rich selenium type northeast Chinese caterpillar fungus:
The silkworm chrysalis that body surface is disinfected is put in the Cans after the sterilization, and every bottled 5-10 pupa with the several holes of inoculation pinprick (3-5), is poured second-class liquid isolate 5ml in the Cans that fill silkworm chrysalis under aseptic condition then, builds polyester film, shakes up.Place 18-22 ℃, the light that overflows is cultivated down, when treating that fruit body primordium forms, uses syringe, extracts 3ml, the selenium for preparing in advance, germanium.The zinc aqueous solution (concentration is 4000ppm) sprays to length to be had on the silkworm chrysalis of fruit body primordium, grows up to through 15 days left and right sides sporophores.Cultivate Chinese caterpillar fungus in this way, it contains the Chinese caterpillar fungus that zinc, germanium, selenium amount cultivates than general method and carries 3-15 doubly, and output improves 6-30%.
5. in booth, cultivate rich zinc, germanium, selenium type northeast Chinese caterpillar fungus
Because DBC-888 bacterial strain adaptive faculty and anti-infection ability are strong, can carry out big area and cultivate.Its method is as follows: under aseptic condition, getting DBC-888 secondary bacterium liquid 0.2ml is expelled in silkworm chrysalis (tussah, the silkworm) body that body surface disinfects, the silkworm chrysalis of taking over kind is put the people to go out in the Cans of bacterium, Intake Quantity is 2/3rds of Cans, seal with polyester film, place Cans 20-25 ℃ to cultivate 7 days down.The pupa that to grow mycelia is again put into the compost of warmhouse booth, leaf on the upper cover, and the spacing of pupa is 50-70mm.The temperature of booth is controlled at 17-22 ℃, relative humidity 90-95%, regularly ventilation.Grew and the similar stroma of wild northeast Chinese caterpillar fungus through 15-25 days.Spray to stroma with the 4000ppm germanium, zinc, the selenium aqueous solution that prepare when stroma just is unearthed, after 10-15 days, the stroma maturation was in time gathered.
The rice for preparing nutritive medium can also be packed in polyester or the polypropylene plastics bag, through autoclaving, behind the inoculation DBC-888, place 20-25 ℃ to cultivate two weeks down, rice covers with mycelia, places the compost of above-mentioned condition warmhouse booth, also grows and the similar stroma of wild northeast worm in 25-40 days.Spray to stroma with the 4000ppm germanium, zinc, the selenium aqueous solution that prepare when stroma just is unearthed, after 10-15 days, the stroma maturation was in time gathered.Also can select to cultivate with aforesaid method in the soil of the hillside thick forest that suits.
Effective ingredient extracting method in the three .DBC-888 northeast Chinese caterpillar funguses:
For the extracting method of northeast Chinese caterpillar fungus, current still problem of studying generally all takes conventional alcohol immersion to extract the hot-water soak extracting method.The present invention is with the new fresh sporophore of northeast Chinese caterpillar fungus, grinds, and carries out press filtration with the deckle board strainer, filtrate is placed in the refrigerator and preserves, and filter residue is used ethanol again, and filtrate is extracted in hot-water soak, filtrate in this filtrate and the refrigerator is mixed into the Chinese caterpillar fungus extracting solution, and this extracting solution can be made into the healthcare products of various formulations.Also can cooperate and make health promoting wine, oral liquid, pulvis, health tea etc. with other herbal medicine.
Four. northeast cordyceps product and technology thereof
The extracting solution that the precious senior tonic solution of king among the present invention is exactly the northeast Chinese caterpillar fungus adds herbal medicine and makes.The precious senior tonic solution of king is according to motherland's medical treasure-house, promotes longevity, and the secret recipe of kidney invigorating and YANG supporting, the fresh DBC-888 Chinese caterpillar fungus that is equipped with a kind of special cultivation is a main raw material, adopts modern biotechnology to make with extra care and forms.This product integrates nourishing, keeps healthy, treats, and merit is the immunologic function of nourishing and strengthening vital, Ginseng Extract, raising human body, removes free radical, improves the old ability of the anti-sorrow of body.
Motherland's medical science is thought: the kidney being the origin of congenital constitution, kidney storing essence, kidney essense are the base substances of human development's reproduction, be called as human life this, the prosperity and decline of life often has direct relation with the function power of kidney.And the precious senior tonic solution of king effects a permanent cure with nourishing just.
The characteristics of the precious senior tonic solution of king:
(1) difference of the precious senior tonic solution of king and other kidney invigorating and YANG supporting medicine is that the nourishing Chinese medicine that the medicinal material selected for use in the prescription is preciousness belongs to pure biological products, the Chinese medicine that does not contain any side effect, do not contain pharmaceutical chemicals and artificial color and narcotic, to the without any side effects and bad reflection of human body, be the reliable nutrient health of non-toxic and safe, the anti-station toxicological test of establishing proves to human body it is safe and reliable through the Shenyang City.
(2) raw material sources conveniently are easy to get.
(3) collocation of holozoic nutrition and holophytic nutrition is reasonable in the prescription, and science has played the good action of having complementary advantages.
(4) the maximum characteristics of the precious senior tonic solution of king are that used northeast Chinese caterpillar fungus is the bright Chinese caterpillar fungus of cultivating with special methods in the prescription.
(5) the precious senior tonic solution nutrition of king is abundant and content is high, be general healthcare products can not compare.Wherein contain 18 seed amino acids, make the aspartic acid content of health Ginseng Extract reach 526mg/L, the L-glutamic acid content that increases brain cell activity is 397mg/L, the arginine of sexual function improvement is arranged, zinc, germanium, selenium etc.Cordycepin, Cordyceps polysaccharide, selenium, germanium etc. anticancer, anti-cancer are arranged, the SOD, carotene, VE, zinc of anti-aging effects etc. are arranged, Vpp, oleic acid, the linolic acid of treatment, health care cardiovascular systems arranged.Its content sample is seen laboratory test report.From nutrient contg, be 2-30 times of the famous long-lived permanently happy tonic wine in China and foreign countries.(auspicious see laboratory test report).
Embodiment 1: senior tonic solution is formed and blending process:
Form:
Genseng 1.5-6% Chinese caterpillar fungus extracting solution 0.5-5%
Radix Astragali 2-5% pallas pit viper 0.5-2%
Herba Epimedii 3-6% male Bombycis mori 1-5%
Radix Angelicae Sinensis 1-4% animal genital (donkey, dog, deer whip) 0.5-2%
Bark of eucommia 1-5% pilose antler 0.3-1%
Matrimony vine 2-5% desert cistanche 0.5-2%
Tuber Fleeceflower Root 1-5% cynomorium songaricum 1-5% (available Root of Medicinal Indian mulberry generation)
Spend soaking in Chinese liquor 3-6 after individual month by the 14 flavor Chinese medicines (except that the Chinese caterpillar fungus extracting solution of northeast) that above-mentioned (1) is formed with 60, filter and in filtrate, add northeast Chinese caterpillar fungus extracting solution 0.5-5% again, with rock sugar 8%, with seasonings 1% tonic solution.
Embodiment 2: the blending process of king's Bao oral liquid
Press the flavor Chinese medicine of 14 in the foregoing description 1 (except that the Chinese caterpillar fungus extracting solution of northeast) and spend liquor with 60, after soaking 3-6 month, filter, filtrate through distill concentrate remove ethanol after, add extracting solution 0.5-5%, rock sugar 8% that a certain amount of spring (water yield is suitable with the amount of alcohol of removing) adds the northeast Chinese caterpillar fungus again, king's Bao oral liquid.
Check:
Prepare the precious senior tonic solution of king, oral liquid, every index: nutritions such as amino acid, VITAMIN, trace element, cordycepin, 80D, Cordyceps polysaccharide, oleic acid, linolic acid, calabash radish element meet the company standard acceptance(check) through chemical examination.
Packing and sterilization:
After product is qualified, under aseptic condition,, be sub-packed in the bottle of clean different size oral liquid, tonic solution.Carry out sterilising treatment (with pasteurizer sterilization, 75-80 ℃ condition following 20 minutes) and carry out bacterium inspection, qualified back decals.
Store: oral liquid is preservation period half a year at low temperatures
Preservation period is 2 years under the senior tonic solution normal temperature.
Northeast Chinese caterpillar fungus DBC-888 bacterial classification has been preserved in BeiJing ZhongGuanCun China Committee for Culture Collection of Microorganisms common micro-organisms center on September 23rd, 1993.Preserving number: CGMCC NO.0200.
Claims (7)
1, a kind of method of cultivating rich zinc type northeast Chinese caterpillar fungus DBC-888 is characterized in that:
(1) substratum or title nutritive medium contain (by 100% weight): potassium primary phosphate 1%, and sal epsom 0.5%, millet swill 2%, animal blood 1%, all the other are soya-bean milk;
(2), get rice 40-50g, white squash seed meal 0.1-1%, Herba Epimedii 0.1-0.05% is put in the clean Cans of people, add and state nutritive medium 35-55g, pack in the Cans, with the 360-900mg zinc additive water-soluble after, also add in the Cans, do lid with mylar, tighten autoclaving (1.2kg/cm2) 30 minutes, inoculation when temperature drops to 20 ℃;
(3) will grow good northeast Chinese caterpillar fungus second-class liquid isolate and draw 10ml with special Glass tubing inoculator, under aseptic condition, be inoculated in the above-mentioned Cans, build mylar again, put to 15-20 ℃ of culturing room and cultivate, generally will grow the northeast Chinese caterpillar fungus of orange in 40-60 days.
Northeast Chinese caterpillar fungus DBC-888 bacterial classification is preserved in BeiJing ZhongGuanCun China Committee for Culture Collection of Microorganisms common micro-organisms center on September 23rd, 1993, and preserving number is CGMCCNO.0200.
2, a kind of method of cultivating rich selenium type northeast Chinese caterpillar fungus DBC-888 is characterized in that:
(1) substratum or title nutritive medium contain (100% weight meter) potassium primary phosphate 1%, sal epsom 0.5-2%, and VB10.5-2%, animal blood 1%, all the other are soya-bean milk;
(2) get rice 35-40g, astragalus membranaceus powder 0.5-3.0g packs in the clean Cans, adds above-mentioned nutritive medium 35-45g, in the Cans of packing into, 360-900mg selenium additive also is added in the Cans after water-soluble again, seals mouth with mylar, and autoclaving is reduced to normal temperature;
(3) will grow good northeast Chinese caterpillar fungus second class inoculum and be inoculated in the Cans, and be placed in 17-20 ℃ and cultivated 40-60 days, grow stroma.
3, a kind of method of cultivating rich germanium type northeast Chinese caterpillar fungus DBC-888 is characterized in that:
(1) substratum or nutritive medium contain (by 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, VB10.5-2%, animal blood 1%, and all the other are soya-bean milk;
(2) get rice 35-40g, add Ganoderma powder or join powder 0.2-1.0g in vain, and add above-mentioned nutritive medium 35-50g in Cans, get the water-soluble back of 0.015-2mg germanium additive again and add in the Cans, seal with polyester film, autoclaving is reduced to the normal temperature inoculation;
(3) will grow good northeast Chinese caterpillar fungus second class inoculum and be inoculated in the Cans, be seated among the 17-20OC, and cultivate 40-60 days, grow sporophore.
4, the cultural method described in claim 1,2 or 3, it is characterized in that: the zinc, selenium or the germanium additive that add different concns in the substratum wherein are water-soluble inorganic zinc, inorganic selenium or inorganic Ge, absorb resin, protein, the youngster's fourth matter that transforms and be enriched in cell by mycelium, sporophore, change through physiochemistry in polysaccharide and the nucleic acid, make inorganic elements become organism, make it to be easy to be absorption of human body.
5, a kind ofly on silkworm chrysalis, cultivate rich zinc, rich germanium, the method of rich selenium type northeast Chinese caterpillar fungus DBC-888, it is characterized in that: the silkworm chrysalis that body surface is disinfected is put in the Cans after the sterilization, every bottled 5-10 pupa, under aseptic condition, with inoculating needle silkworm is leapt up and to prick 3-5 hole, to grow good northeast Chinese caterpillar fungus second-class liquid isolate 5ml then pours in the Cans that fill silkworm chrysalis, build polyester film, shake up, place 18-22 ℃, the light that overflows is cultivated down, when treating that fruit body primordium forms, extract the selenium that 3ml prepares in advance with syringe, germanium, the zinc aqueous solution, spraying to length has on the silkworm chrysalis of fruit body primordium, promptly grows up to Cordyceps sporophore through about 15 days.
6, a kind ofly in warmhouse booth, cultivate rich zinc, rich germanium, the method of rich selenium type northeast Chinese caterpillar fungus DBC-888, it is characterized in that: under aseptic condition, get long good northeast Chinese caterpillar fungus secondary bacterium liquid 0.2ml and be expelled to the silkworm chrysalis that body surface disinfects, as tussah, in the silkworm body, put into the Cans of the bacterium of going out with taking over the silkworm chrysalis of planting, Intake Quantity is 2/3rds of Cans, seal with polypropylene film, place Cans 20-25 ℃ to cultivate 7 days down, the pupa that to grow mycelia is again put into the compost of warmhouse booth, leaf on the upper cover, and the spacing of pupa is the 50-70 millimeter, the temperature of booth is controlled at 17-20 ℃, relative humidity 90-95%, regularly ventilation is when stroma just is unearthed, with the zinc for preparing, germanium, the aqueous solution of selenium is sprayed on the fruit body primordium, buys body through about 15 days sons and grows.
7, extract the method for northeast Chinese caterpillar fungus DBC-888 effective ingredient, it is characterized in that: the sporophore among the claim 1-3 is ground, with the press filtration of deckle board strainer, filtrate is placed in the refrigerator and preserves, filter residue is used ethanol again, and filtrate is extracted in hot-water soak, it is mixed with the filtrate in the refrigerator, become northeast Chinese caterpillar fungus extracting solution; It can make the healthcare products of various formulations, also can cooperate with other herbal medicine and make health promoting wine, oral liquid, pulvis or health tea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93117581A CN1091149C (en) | 1993-09-24 | 1993-09-24 | Method for cultivating Chinese caterpillar fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93117581A CN1091149C (en) | 1993-09-24 | 1993-09-24 | Method for cultivating Chinese caterpillar fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1100754A CN1100754A (en) | 1995-03-29 |
| CN1091149C true CN1091149C (en) | 2002-09-18 |
Family
ID=4992093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93117581A Expired - Fee Related CN1091149C (en) | 1993-09-24 | 1993-09-24 | Method for cultivating Chinese caterpillar fungus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1091149C (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375784C (en) * | 2006-01-23 | 2008-03-19 | 孟震 | Complex function type northern Chinese caterpillar fungus and its production method |
| CN100434508C (en) * | 2006-11-08 | 2008-11-19 | 中山大学 | Method for increasing yield of Qinghai Tibet plateau cordyceps |
| CN101731357B (en) * | 2009-11-16 | 2013-11-27 | 曾帅 | Method for preparing wormgrass soymilk |
| CN102212581A (en) * | 2010-04-12 | 2011-10-12 | 李鹄鸣 | Preparation method and application of cordyceps polysaccharide germanium |
| CN102150639A (en) * | 2010-12-22 | 2011-08-17 | 李树怀 | Cordyceps sinensis selenium-enriched health-care egg producing method |
| CN102550290B (en) * | 2012-01-17 | 2015-06-10 | 辽宁华夏三宝生物资源科技开发有限责任公司 | Method for cultivating cordyceps militaris strain |
| CN103371050A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Method for cultivating cordyceps sinensis |
| CN102613368A (en) * | 2012-04-24 | 2012-08-01 | 福建农大科技开发总公司 | North cordyceps sinensis herba epimedii compound tea bag product and producing method thereof |
| CN103621315B (en) * | 2013-12-12 | 2015-02-04 | 福建农林大学 | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution |
| CN104756759B (en) * | 2015-03-17 | 2017-01-04 | 沁阳市西向食用菌研究所 | A kind of Rhizoma Dioscoreae Cordyceps and cultural method thereof |
| CN105746172A (en) * | 2016-02-23 | 2016-07-13 | 张家港顺泰元生物科技有限公司 | Selenium-enriched cordyceps culture method |
| CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
| CN106520562A (en) * | 2016-10-14 | 2017-03-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for cultivating caterpillar fungus by using ginseng type extract and application |
| CN107960270A (en) * | 2017-08-31 | 2018-04-27 | 广西沙田仙人滩农业投资有限公司 | A kind of method of cordyceps militaris cultivation |
| CN112166954A (en) * | 2020-10-27 | 2021-01-05 | 苏州家和蚕业生物科技有限公司 | Culture method of cordyceps militaris |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1019816B (en) * | 1987-10-13 | 1992-12-30 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1069658A (en) * | 1992-09-02 | 1993-03-10 | 鞍山保健饮品制造公司 | Cordyceps sonensis oral liquid |
| CN1094754A (en) * | 1993-09-12 | 1994-11-09 | 李江 | Method for artificially culturing selenium-rich Northern Chinese caterpillar fungus |
-
1993
- 1993-09-24 CN CN93117581A patent/CN1091149C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1019816B (en) * | 1987-10-13 | 1992-12-30 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1069658A (en) * | 1992-09-02 | 1993-03-10 | 鞍山保健饮品制造公司 | Cordyceps sonensis oral liquid |
| CN1094754A (en) * | 1993-09-12 | 1994-11-09 | 李江 | Method for artificially culturing selenium-rich Northern Chinese caterpillar fungus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1100754A (en) | 1995-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106977320B (en) | Culture medium for needle mushrooms and preparation method thereof | |
| CN1091149C (en) | Method for cultivating Chinese caterpillar fungus | |
| CN104187947B (en) | A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. liquid fermenting beverage and its preparation method | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN101372671B (en) | Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof | |
| TWI459953B (en) | A composition to improve cordycepin of solid-state cultivation | |
| KR20130057507A (en) | A method of cultivation cordyceps militaris | |
| KR20210058759A (en) | Method for obtaining mass proliferation of Cordyceps sinensis and mass culture method using it | |
| CN1331908A (en) | Chinese herbal medicinal edible fungus culture medium and edible fungus producing method with the culture medium | |
| CN106479903A (en) | A kind of manufacture method of live body glossy ganoderma dish garden | |
| CN104054504B (en) | The breeding method of bee pupa Chinese caterpillar fungus | |
| KR20010027806A (en) | Culturing method of cordyceps spp | |
| CN100575475C (en) | The method of wheat cultivation Cordyceps militaris | |
| CN107873391B (en) | Fungus inhibiting culture medium for cultivating agaric and cultivation method thereof | |
| CN116515641B (en) | Hericium coralloides and application thereof | |
| CN111869507A (en) | Method for cultivating cordyceps sinensis by taking passion fruit as container | |
| CN1462591A (en) | Food and substrate of cordyceps militatris, and preparing method | |
| CN103392513B (en) | Ferment of aweto mycelia and application of ferment | |
| KR100723068B1 (en) | Method for culturing flammulina velutipes including ginseng saponin | |
| CN105901158B (en) | A kind of preparation method of cordyceps sinensis bean curd | |
| CN1536069B (en) | Method for large-scale production of catepillar fungus and lucid ganoderma | |
| KR100762819B1 (en) | A method for effective fruiting body production of paecilomyces japonica using bombus terrestris and preparation of healthy food comprising the same | |
| KR100442766B1 (en) | Artificial method to culture Cordyceps Kyushuensis Y.Kobayas and the application for its sporocarp | |
| KR20020072878A (en) | Method for solid culturing of hyphostroma and carpophore of hericicum erinaceus using a medicinal plant and food to help health including abstract of hyphostroma and carpophore | |
| CN112314918A (en) | Cordyceps sinensis and chestnut paste |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhao Fengying Document name: Notification of Termination of Patent Right |
|
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |