CN109055277A - 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 - Google Patents
一种从土壤中筛选嗜酸耐重金属固氮菌的方法 Download PDFInfo
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- CN109055277A CN109055277A CN201811081231.4A CN201811081231A CN109055277A CN 109055277 A CN109055277 A CN 109055277A CN 201811081231 A CN201811081231 A CN 201811081231A CN 109055277 A CN109055277 A CN 109055277A
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 54
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 18
- 239000013049 sediment Substances 0.000 claims description 14
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 8
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000011565 manganese chloride Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 4
- HDSBZMRLPLPFLQ-UHFFFAOYSA-N Propylene glycol alginate Chemical compound OC1C(O)C(OC)OC(C(O)=O)C1OC1C(O)C(O)C(C)C(C(=O)OCC(C)O)O1 HDSBZMRLPLPFLQ-UHFFFAOYSA-N 0.000 claims description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 4
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 4
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- 239000011780 sodium chloride Substances 0.000 claims description 4
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- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 239000011686 zinc sulphate Substances 0.000 claims description 4
- 229910021556 Chromium(III) chloride Inorganic materials 0.000 claims description 3
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 claims description 3
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- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims 1
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- 229910052751 metal Inorganic materials 0.000 description 5
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- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910052603 melanterite Inorganic materials 0.000 description 4
- 229910052564 epsomite Inorganic materials 0.000 description 3
- HWSZZLVAJGOAAY-UHFFFAOYSA-L lead(II) chloride Chemical compound Cl[Pb]Cl HWSZZLVAJGOAAY-UHFFFAOYSA-L 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000284 extract Substances 0.000 description 2
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- 239000000047 product Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
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- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
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- 239000003337 fertilizer Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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CN201811081231.4A CN109055277B (zh) | 2018-09-17 | 2018-09-17 | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 |
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CN201811081231.4A CN109055277B (zh) | 2018-09-17 | 2018-09-17 | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112745860A (zh) * | 2021-02-23 | 2021-05-04 | 潍坊科技学院 | 一种基于石墨烯的酸化土壤调理剂 |
CN113337419A (zh) * | 2021-04-26 | 2021-09-03 | 李雪琛 | 一种土壤酸化地区用微生物菌剂的制备方法 |
CN115820493A (zh) * | 2022-12-01 | 2023-03-21 | 安徽农业大学 | 一种固氮菌富集和用纤维素培养固氮菌降解染料的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104862250A (zh) * | 2015-05-08 | 2015-08-26 | 周洪波 | 一种嗜酸铁氧化微生物复合菌剂及其制备方法 |
CN108018250A (zh) * | 2018-01-29 | 2018-05-11 | 武汉新禹智水环保科技有限公司 | 一株嗜酸氧化亚铁硫杆菌及其在环境治理中的应用 |
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2018
- 2018-09-17 CN CN201811081231.4A patent/CN109055277B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104862250A (zh) * | 2015-05-08 | 2015-08-26 | 周洪波 | 一种嗜酸铁氧化微生物复合菌剂及其制备方法 |
CN108018250A (zh) * | 2018-01-29 | 2018-05-11 | 武汉新禹智水环保科技有限公司 | 一株嗜酸氧化亚铁硫杆菌及其在环境治理中的应用 |
Non-Patent Citations (2)
Title |
---|
GENE W,ET AL: "Genome-Directed Isolation of the Key Nitrogen Fixer Leptospirillum ferrodiazotrophum sp.nov.froma an Acidophilic Microbial Community", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
王婧: "几种特定生物学功能的微生物的筛选与鉴定", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112745860A (zh) * | 2021-02-23 | 2021-05-04 | 潍坊科技学院 | 一种基于石墨烯的酸化土壤调理剂 |
CN113337419A (zh) * | 2021-04-26 | 2021-09-03 | 李雪琛 | 一种土壤酸化地区用微生物菌剂的制备方法 |
CN113337419B (zh) * | 2021-04-26 | 2023-06-13 | 沈阳华清源农业发展有限公司 | 一种土壤酸化地区用微生物菌剂的制备方法 |
CN115820493A (zh) * | 2022-12-01 | 2023-03-21 | 安徽农业大学 | 一种固氮菌富集和用纤维素培养固氮菌降解染料的方法 |
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Address after: 230000 No.20 Tongling Road, Yaohai District, Hefei City, Anhui Province Patentee after: Anhui Construction Engineering Transportation and Navigation Group Co.,Ltd. Patentee after: ANHUI University Patentee after: ANHUI CONSTRUCTION ENGINEERING GROUP HOLDINGS Co.,Ltd. Address before: 230011 No. 20, Tongling Road, Hefei, Anhui Patentee before: ANHUI PROVINCE TRANSPORTATION HARBOR ENGINEERING CO.,LTD. Patentee before: ANHUI University Patentee before: ANHUI CONSTRUCTION ENGINEERING GROUP HOLDINGS Co.,Ltd. |