CN108966741A - A method of improving Rhus succedanea percentage of seedgermination and quick pregermination - Google Patents
A method of improving Rhus succedanea percentage of seedgermination and quick pregermination Download PDFInfo
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- CN108966741A CN108966741A CN201810932509.8A CN201810932509A CN108966741A CN 108966741 A CN108966741 A CN 108966741A CN 201810932509 A CN201810932509 A CN 201810932509A CN 108966741 A CN108966741 A CN 108966741A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a kind of raising Rhus succedanea percentage of seedgermination and the methods of quick pregermination, the following steps are included: (1) chooses the Rhus succedanea seed of mature and plump, removal pericarp simultaneously carries out seed disinfection, then impregnates vernalization in 20-25 minutes with 98% concentrated sulfuric acid；(2) it by step (1) resulting seed, is impregnated 12 hours with 300mg/L Gibberellins solution, is rinsed well under flowing water after having handled, control condition of culture carries out vernalization.The present invention can be handled without long-time seed soaking and stratification, quickly broken Rhus succedanea seed dormancy, effectively improved percentage of seedgermination, germination percentage is up to 98% or more.And germinating time is greatly shortened, and it can all sprout within about 7-10 days, achieve the purpose that quick pregermination, neat and consistent of germinateing, convenient for operational administratives such as later period seedling replanting field planting.
Description
Technical field
The invention belongs to plant seed germination pretreatment technology fields, sow in particular to a kind of Rhus succedanea
The quick pregermination method of nursery handles breaking dormancy more particularly, to a kind of Rhus succedanea seed concentrated sulfuric acid combination gibberellin
It improves germination percentage and reaches the method for quick pregermination.
Background technique
Rhus succedanea (Toxicodendron succedaneum) is that Anacardiaceae (Anacardiaceae) paint belongs to
(Toxicodendron) deciduous tree or dungarunga are that the Rhus succedanea that Japan introduces from China before more than 400 years is improved by long-term
The new varieties cultivated.The tree-like grace of Rhus succedanea, autumn leaf color is red gorgeous beautiful, and intensive branch end, color is pure, is a kind of
Excellent Landscape Trees.It simultaneously because of its adaptable, drought-resistant barren, well developed root system, and is excellent water and soil conservation tree
Kind.Rhus succedanea, which integrates beautiful color leaf and adopts seed, produces wax, is to have widely used excellent economic tree.Paint seed crust
On have coating of wax matter, with endocarp phase joint, endocarp is hard, yellowish-brown, slightly referred to finishing after product be known as Japan
Haze tallow, therefore Rhus succedanea is also the main plant for producing haze tallow and raw lacquer.The haze tallow extracted from its fruit is a kind of day
Right biowax, the excellent spy such as suppleness, adhesive force, glossiness that because being rich in dicarboxylic acids there are other biowaxes to hardly match
Property, it is widely used in cosmetics, medicine, paint, waterproofing agent, e-inks, wax and spins stamp, industrial lubricants and other many
Professional domain.
Rhus succedanea paint seed kind skin has wax, and seed is hard, and permeable breathable is poor, seed propagation nature germination percentage pole
It is low, and required time is long, germinates irregular, growth of seedling is inconsistent.Its germination percentage and germination quality are improved, it is necessary before sowing
Carry out presprouting of seeds pre-treatment.
The concentrated sulfuric acid is easy to get as conventional seed treatment medicament, low in cost, and dense sulfuric acid treatment method is easy,
Germination is neat, and the time is short, and germination percentage is high.Dense sulfuric acid treatment seed blackberry, blueberry, gleditsia sinensis, etc. be widely used in plants, have preferably
Effect.Gibberellin is a kind of wide spectrum, efficient plant growth regulator, has stratification and shoot and leaf growth and ahead of time to plant
The effects of yielding positive results.
Have many using the concentrated sulfuric acid or gibberellin vernalization in current research, but Stratificated treatment will be passed through greatly, and
And only the document report of the concentrated sulfuric acid and gibberellin combination processing lacquer tree seed, such as Yuan Yaqi " are not existed together without disclosing
Manage the influence sprouted to 3 provenance lacquer tree seeds " (" economic forest research ", 2017,35 (3)), Tang Li etc. is " at different chemical agents
Manage influence to Rhus succedanea percentage of seedgermination " (guangdong agricultural science, the 2011, the 10th phase), " the different disposal method pair such as Yang Weize
The influence that lacquer tree seed is sprouted " (Anhui forestry science and technology, 2014,40 (6): 30-33).
Summary of the invention
In view of the deficiencies in the prior art, inventor creatively mentions according to Rhus succedanea characteristic and required condition of germinateing
For a kind of method that suitable Rhus succedanea improves germination percentage and quick pregermination.
In order to achieve the object of the present invention, inventor passes through a large number of experiments the study found that the concentrated sulfuric acid and gibberellin handle day
The key factor of this Rhus succedanea presprouting of seeds is controlled concentration and time, and is finally obtained following technical solution:
A method of Rhus succedanea percentage of seedgermination and quick pregermination are improved, method includes the following steps:
(1) the Rhus succedanea seed for choosing mature and plump removes pericarp and carries out seed disinfection, then with 98% dense sulphur
Acid soak 20-25 minutes, seed is rinsed well with flowing water after having handled；
(2) it by step (1) resulting seed, is impregnated 12-15 hours with 200-400mg/L Gibberellins solution, after having handled
It rinses well, dries under flowing water, using germinating box germination hastening method vernalization.
The method of above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, wherein in step (1), 10 below the moon
The seed with pericarp that early November in ten days-selects the elite stand acquisition of robust growth mature, with 60-70 DEG C of hot-water soak, is washed by rubbing with the hands,
Pericarp and impurity are removed, removes the seed for swimming in water surface upper layer with wet cleaning process, retains and sinks to water-bed full seed, drain the water
Point, it dries, is stored at room temperature aeration-drying spare at shady and cool ventilation.
The method of above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, wherein in step (1), seed disinfection
It is impregnated 1-2 hours using 50% carbendazim, 500 times of liquid, then clear water is cleaned and drained.
The method of above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, it is dense wherein in step (1) and (2)
The amount of sulfuric acid and Gibberellins solution will be higher by Seed Levels face 1-2cm, and being stirred continuously in treatment process keeps seed abundant with medicament
Contact, is rinsed well seed with flowing water after having handled.
The method of above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, wherein in step (2), described is red
The concentration of mycin solution is preferably 300mg/L, and soaking time is 12 hours.
The method of above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, wherein in step (2), germination
20 DEG C of case set temperature, the photoperiod is the reaction of 16h/8h brightness, and intensity of illumination 3500Lx checks ventilation daily, and keeps planting
Son is wet.
It should be noted that the method for above-mentioned raising Rhus succedanea percentage of seedgermination and quick pregermination, wherein step
(1) and in (2), seed disinfection mainly will carry out disinfection processing to exosper before dense sulfuric acid treatment, guarantee that seed is being sent out
It is unlikely to go rotten during bud and addles.And gibberellin processing will promote kind of skin cracking, water suction, an expansion after dense sulfuric acid treatment,
It plays a role so that gibberellin is contacted with seed.
Compared with prior art, the method for Rhus succedanea seed quick pregermination of the present invention have the following advantages that and
Significant progress:
(1) method of Rhus succedanea seed quick pregermination of the invention can effectively improve Rhus succedanea germination
Rate, net kind before sowing, selected and disinfection it is secure to germination and seedling quality.
(2) method of Rhus succedanea seed quick pregermination of the invention it can be seen from the embodiment of the present invention 1,
Germination percentage is up to 95% or more, and seedling late growth is good.
(3) it is greatly improved germination percentage after concentrated sulfuric acid combination gibberellin long time treatment, while shortens germinating time, the two
Performance can be cooperateed with to break the effect of Rhus succedanea seed physiology suspend mode, seed can be promoted to originate bud time advance, and it is whole to germinate
Homogeneous causes.
(4) concentrated sulfuric acid is easy to get as conventional seed treatment medicament, low in cost, and the concentrated sulfuric acid combines for a long time
The method of gibberellin processing washes that the methods of dewaxing is easy to operate, and sowing quantity is small, time saving and energy saving by rubbing with the hands compared with hot water dewaxing and manually, favorably
In realization plant layout nursery.
(5) long the time required to conventional Stratificated treatment, it is easy to produce the mold of seed in lamination process and rots, viability decline,
Also it will affect the seedling quality of later period germination.Gibberellin replaces Stratificated treatment, substantially reduces the time of low temperature or sand hiding, reduces
Rotten possibility during earthing of seeds is also that production strong sprout lays the foundation while improving operating efficiency.
Detailed description of the invention
Fig. 1 is influence result figure of the different solutions processing to Rhus succedanea germination；
Fig. 2 is influence result figure (1: dense sulfuric acid treatment after seed wax of the dense sulfuric acid treatment to Rhus succedanea germination
Matter layer corrosion spalling；2: growth of seedling is healthy and strong after dense sulfuric acid treatment；3: the left side is the seed of clear water processing, and epidermis densification is impermeable
Water, the right are the seed after dense sulfuric acid treatment, and breach is fast, and germination is early, and the obvious water swelling of seed becomes larger.)
Fig. 3 is that concentrated sulfuric acid combination gibberellin handles Rhus succedanea germination neat and consistent result figure (No. 1 and No. 2
Culture dish is 200mg/L gibberellin；3,4, No. 5 culture dishes are 300mg/L gibberellin；6, No. 7 culture dishes are that 400mg/L is red mould
Element.)
Fig. 4 is that gibberellin handles time Rhus succedanea germination neat and consistent influence result figure.
Specific embodiment
It is specific embodiment of the present invention below, technical solution of the present invention is described further, but this hair
Bright protection scope is not limited to these examples.It is all to be included in this without departing substantially from the change of present inventive concept or equivalent substitute
Within the protection scope of invention.
Influence of 1 different solutions of the embodiment processing to Rhus succedanea germination
The Rhus succedanea seed for selecting mature and plump, uses clear water after 500 times of 50% carbendazim liquid soaking disinfection 1 hour
It cleans and drains.Washing powder solution, saleratus solution and the concentrated sulfuric acid (i.e. 98%H are used respectively2SO4) solution impregnate seed, usage amount with
Not crossing seed 1cm high is advisable, and being stirred continuously comes into full contact with medicament with seed.If test group 1, test group 2, test group 3 and sky
White group, every group of 30 seeds are repeated 3 times.Test group 1 is dense sulfuric acid treatment 25min, and test group 2 is 1% saleratus solution 12h,
Test group 3 is that 1% washing powder handles 12h.Blank group is that clear water impregnates for 24 hours.Liquid medicine is removed after having handled, is placed in flowing
It is rinsed 30 minutes under tap water, cleans seed.Then 300mg/L GA is used3Solution impregnates 12 hours, clean seed after having handled
It being placed in illumination box, sets 20 DEG C of incubator temperature, the photoperiod is that 16h/8h brightness is reacted, intensity of illumination 3500Lx,
Ventilation is checked daily, and keeps seed wet, is observed and is counted germination percentage.
Test result: 1 germination percentage of test group is up to 95% or more, has seed breach broken shell after processing 1 day, has within the 3rd day
Seed exposes radicle and sprouts, and all hair is neat for seed after about 10 days, and growth of seedling is healthy and strong good, neat and consistent of emerging；Test group 2 is sent out
Bud rate is 32%, has within the 8th day seed to expose radicle and sprouts, germinates the duration about 12 days, emerge more neat；Test group 3 is germinateed
Rate is 28%, has within the 10th day seed to expose radicle and sprouts, germinates the duration about 15 days, emerge irregular.It can be seen that dense sulphur
The processed seed breach shell-breaking rate of acid is high, and germinating time is short, and germination percentage is high.The concentrated sulfuric acid can beat the mechanical obstacles to break in the seed coat, promote
Into seed water swelling, to be rinsed well in time after processing, seed not will receive concentrated sulfuric acid harm, and growth of seedling is in good condition, at
Seedling rate is high.Processing of the dense sulfuric acid treatment compared with sodium bicarbonate and washing powder can germinate 7-10 days ahead of time, can do sth. in advance compared with clear water immersion treatment
It germinates more than 20 days.Statistical result is shown in Table 1.
Influence of 1 different solutions of the table processing to Rhus succedanea germination
Influence of the various concentration and time of 2 dense sulfuric acid treatment of embodiment to Rhus succedanea germination
The Rhus succedanea seed for selecting mature and plump, uses clear water after 500 times of 50% carbendazim liquid soaking disinfection 1 hour
It cleans and drains.Then the dense sulfuric acid treatment for using various concentration and time, if test group 1, test group 2, test group 3, test group
4, test group 5, test group 6 and test group 7, every group of 30 seeds are repeated 3 times.Wherein test group 1 is 60%H2SO4Handle seed
30 minutes, test group 2 was 60%H2SO4Processing seed 60 minutes, test group 3 are 60%H2SO4Processing seed 90 minutes, test
Group 4 is 98%H2SO4Processing seed 10 minutes, test group 5 are 98%H2SO4Processing seed 15 minutes, test group 6 are 98%
H2SO4Processing seed 20 minutes, test group 7 are 98%H2SO4Processing seed 25 minutes.Other processing are the same as embodiment 1.
Test result: dense sulfuric acid treatment is greatly improved percentage of seedgermination, increase and place with dense sulfuric acid treatment concentration
The extension of time is managed, germination percentage has certain increase, and it is each to handle the equal well-grown of seedling, illustrate that the concentrated sulfuric acid will not be to growth of seedling
It impacts.The germination percentage highest of test group 6 and test group 7, reaches 98% or more, statistical result is shown in Table 2 in embodiment 2.Therefore
Dense sulfuric acid treatment concentration and time are with 98%H2SO4Processing is advisable for seed 20-25 minutes.
Influence of the various concentration and time of 2 dense sulfuric acid treatment of table to Rhus succedanea germination
Influence of 3 various concentration gibberellin of the embodiment processing to Rhus succedanea germination
Early period and dense sulfuric acid treatment are carried out according to the method for test group 6 in embodiment 2, are soaked later with Gibberellins solution
Bubble, if test group 1, test group 2, test group 3 and blank group, every group 30, is repeated 3 times.Test group 1 is 200mg/L gibberellin
Solution seed soaking 12 hours, test group 2 be 300mg/L Gibberellins solution soak seed 12 hours, test group 3 be that 400mg/L gibberellin is molten
Liquid soak seed 12 hours, blank group be clear water soak seed 12 hours.It is disposed to be placed under flowing water and rinse well.Then seed is placed in
In illumination box, 20 DEG C of incubator temperature are set, the photoperiod is that 16h/8h brightness is reacted, intensity of illumination 3500Lx, daily
It checks ventilation, and keeps seed wet, observe and count germination percentage.
Test result: test group 1, test group 2, the test group 3 of various concentration gibberellin processing send out seed compared with blank group
Bud rate significantly improves.Germinating energy is the speed of germination for evaluating seed and the index of germination regularity.It is comprehensively compared with the effect of test group 2
Fruit is best, i.e., 300mg/L Gibberellins solution is soaked seed 12 hours, the results are shown in Table 3.With Yuan Yaqi (2017), Tang Li (2011), Yang Wei
The result of study of damp (2014) et al. is compared: the processing of concentrated sulfuric acid combination gibberellin is better with dense sulfuric acid treatment effect than single, properly
Gibberellin processing, can make seed without Stratificated treatment to break physiological dormancy, improve potentiality of seed, when shortening germination
Between, neat and consistent of emerging.And shakeout hiding or cold stratification time are saved, it at least can do sth. in advance to germinate for 20 days.(bibliography
" influence that different disposal sprouts 3 provenance lacquer tree seeds ", economic forest research, 2017,35 (3), " different Chemical treatments
Influence to Rhus succedanea percentage of seedgermination ", guangdong agricultural science, the 2011, the 10th phase, " different disposal method sprouts lacquer tree seed
The influence of hair " Anhui forestry science and technology, 2014,40 (6): 30-33.)
Influence of 3 gibberellin of the table processing to Rhus succedanea germination
| Group | Germination percentage | Germinating energy | Germination state |
| Test group 1 | 96% | 48% | Germination percentage is high, and germinating energy is medium, germinates more neat |
| Test group 2 | 98% | 56% | Germination percentage is high, and germinating energy is strong, neat and consistent of germinateing |
| Test group 3 | 98% | 51% | Germination percentage is high, and germinating energy is stronger, neat and consistent of germinateing |
| Blank group | 71% | 18% | Germination percentage is lower, and germinating energy is weak, germinates irregular |
Influence of the different gibberellin processing times of embodiment 4 to Rhus succedanea germination
Early period and dense sulfuric acid treatment are carried out according to the method for test group 6 in embodiment 2, are soaked later with Gibberellins solution
Bubble, if test group 1, test group 2, test group 3, test group 4 and blank group, every group 30, is repeated 3 times.Test group 1 is 300mg/
L Gibberellins solution soak seed 2 hours, test group 2 be 300mg/L Gibberellins solution seed soaking 5 hours, test group 3 be 300mg/L red mould
Plain solution seed soaking 8 hours, test group 4 are that 300mg/L Gibberellins solution is soaked seed 12 hours, and blank group is that clear water is soaked seed 12 hours.
It is disposed to be placed under flowing water and rinse well.Then seed is placed in illumination box, sets 20 DEG C of incubator temperature, light week
Phase is the reaction of 16h/8h brightness, and intensity of illumination 3500Lx checks ventilation daily, and keeps seed wet, observes and counts hair
Bud rate.
Test result: test group 1, the test group 2, the germination feelings of test group 3 and blank group of various concentration gibberellin processing
Condition does not have notable difference (referring to fig. 4), but test group 4 is significantly improved compared with the percentage of seedgermination of blank group, is illustrated at gibberellin
It is unobvious to manage short period effect, it is preferable to handle 12 hours effects, the results are shown in Table 4.
4 gibberellin of table handles influence of the time to Rhus succedanea germination
| Group | Germination percentage | Germinating energy | Germination state |
| Test group 1 | 70% | 22% | Germination percentage is low, and germinating energy is weak, germinates irregular |
| Test group 2 | 78% | 28% | Germination percentage is lower, and germinating energy is weak, germinates irregular |
| Test group 3 | 75% | 30% | Germination percentage is lower, and germinating energy is weak, germinates irregular |
| Test group 4 | 98% | 57% | Germination percentage is high, and germinating energy is stronger, neat and consistent of germinateing |
| Blank group | 72% | 19% | Germination percentage is low, and germinating energy is weak, germinates irregular |
Claims (6)
1. a kind of method for improving Rhus succedanea percentage of seedgermination and quick pregermination, which is characterized in that this method include with
Lower step:
(1) the Rhus succedanea seed for choosing mature and plump removes pericarp and carries out seed disinfection, then soaked with 98% concentrated sulfuric acid
Bubble 20-25 minutes, is rinsed well seed with flowing water after having handled；
(2) it by step (1) resulting seed, is impregnated 12-15 hours with 200-400mg/L Gibberellins solution, flowing water after having handled
Under rinse well, dry, using germinating box germination hastening method vernalization.
2. improving the method for Rhus succedanea percentage of seedgermination and quick pregermination according to claim 1, it is characterised in that:
In step (1), in mid-October-early November selection robust growth elite stand acquires the mature seed with pericarp, uses 60-
70 DEG C of hot-water soak is washed by rubbing with the hands, is removed pericarp and impurity, is removed the seed for swimming in water surface upper layer with wet cleaning process, reservation is sunk to
Water-bed full seed, drains away the water, dries at shady and cool ventilation, spare.
3. improving the method for Rhus succedanea percentage of seedgermination and quick pregermination according to claim 1, it is characterised in that:
In step (1), seed disinfection is impregnated 1-2 hours using 50% carbendazim, 500 times of liquid, and then clear water is cleaned and drained.
4. improving the method for Rhus succedanea percentage of seedgermination and quick pregermination according to claim 1, it is characterised in that:
In step (1), step (2), the concentrated sulfuric acid, Gibberellins solution usage amount be liquid level do not had seed 1-2cm.
5. improving the method for Rhus succedanea percentage of seedgermination and quick pregermination according to claim 1, it is characterised in that:
In step (2), the concentration of the Gibberellins solution is 300mg/L, and soaking time is 12 hours.
6. improving the method for Rhus succedanea percentage of seedgermination and quick pregermination according to claim 1, it is characterised in that:
In step (2), 20 DEG C of seed germination box set temperature, the photoperiod is that 16h/8h brightness is reacted, intensity of illumination 3500Lx, daily
It checks ventilation, and keeps seed wet.
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| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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| CN108966741B (en) | 2021-06-18 |
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