CN105265060A - Method for accelerating germination of lithocarpus areca seeds - Google Patents
Method for accelerating germination of lithocarpus areca seeds Download PDFInfo
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- CN105265060A CN105265060A CN201510739464.9A CN201510739464A CN105265060A CN 105265060 A CN105265060 A CN 105265060A CN 201510739464 A CN201510739464 A CN 201510739464A CN 105265060 A CN105265060 A CN 105265060A
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Abstract
The invention discloses a method for accelerating the germination of lithocarpus areca seeds, which is characterized by comprising the following steps of collecting seeds, pretreating the seeds, performing sand-storage germination acceleration, breeding and managing and protecting the seeds, collecting mature lithocarpus areca seeds, performing seed selection, disinfection, seed soaking and gibberellin treatment, performing sand-storage germination acceleration, and obtaining germinant seeds through seed breeding and management and protection. The method for accelerating the germination of the lithocarpus areca seeds disclosed by the invention has the advantages that seed dormancy can be effectively broken only through proper-concentration phytohormone soaking and sand-storage treatment, the seed germination is accelerated, the germination rate is improved, the effects of simple technological operation, low cost, convenience in operation and suitability for popularization are achieved, and better economic benefits, social benefits and ecological benefits are obtained.
Description
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method promoting betel nut Ke seed germination.
Background technology
Betel nut Ke (Lithocarpusareca (Hick.etA.Camus) A.Camus) is Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, and high 10 ~ 15 meters, sprig canescence, without hair, has hole skin.Leaf papery, lanceolar or narrow oblong, long 13 ~ 25 centimetres, wide 3.5 ~ 5.5 centimetres, the narrow point in two ends, base portion is downward, and there is the shallow sharp mouth that splits of minority on leaf margin top or has full edge concurrently, middle arteries and veins is in blade face microprotrusion, 9 ~ 15, the every limit of lateral vein, in blade face micro-pits, offshoot is obvious, two sides is homochromy, has feathering without on hair or arteries and veins armpit; Long 0.5 ~ 1.5 centimetre of petiole.Tassel locusta list fringe armpit is given birth to, and rare short branch forms coniform, long 5 ~ 8 centimetres, and intensive more than spending, filigree is very thin, quite long, and rhachis is very thin; Female inflorescence is long 4 ~ 10 centimetres, normal androgyny, female flower be born in rhachis lower in, therefore infructescence is very short; Every 3 ~ 5 clusters of female flower, usual 1 bow structure is real, gynoecium ovum shape triangle; Bractlet short-term shape when acorn-cup children is tender, extends to 8 millimeters time ripe by long 2 ~ 4 millimeters; The olive-shaped ellipse of nut, or conico-acuminate, high 40 ~ 50 millimeters, wide 20 ~ 35 millimeters, 3 longitudinal directions ridge rib slightly is in obtuse angle arranged at top, top point, Chestnut, without hair, fruit wall thickness 2 ~ 3 millimeters, base portion is smooth, and areola caves in, dark 2 ~ 3 millimeters, bore 8 ~ 15 millimeters.In October at florescence, fruit is ripe for November next year.Betel nut Ke originates in municipal western part of Zhuang National Minority of China (Napo County, Longzhou), the southeast, Yunnan Province (Malipo), and be grown on the area of height above sea level 800 meters to 1,500 meters, be grown in evergreen broad-leaved forest, North Vietnam also has distribution more.
At present, betel nut Ke resource is still in wild distribution, but along with the multifarious demand of protective plant, large-scale artificial cultivation betel nut Ke resource is imperative.Because betel nut Ke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And the betel nut Ke percentage of seedgermination without any hormone agents process in traditional mode of production often only has about 50%, and germinating time evening, emerge irregular, limit the large-scale planting of betel nut Ke.
Summary of the invention
Object of the present invention is the deficiency overcoming existing betel nut Ke Seed germination techniques, provides a kind of germination rate and the quickening germination physiology that can improve betel nut Ke seed, workable, practical simplicity, the method for promotion betel nut Ke seed germination that cost is low.
Technical scheme of the present invention is as follows:
A kind of method promoting betel nut Ke seed germination, comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe betel nut Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe betel nut Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the betel nut Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean betel nut Ke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the betel nut Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir betel nut Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, betel nut Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution betel nut Ke seed after seed soaking being put into 200 ~ 400mg/L soaks 3 ~ 4d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, betel nut Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
Preferably, the betel nut Ke seed in above step (3) and sand are share equally after the ratio of 1:6 is puddled by volume.
Preferably, the even stand thickness described in above step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
Preferably, the periodically sprinkle bactericide described in above step (4) be with volumetric concentration be 1 ‰ carbendazim solutions to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
The present invention relative to prior art, the advantage had and good effect as follows:
1, the method for promotion betel nut Ke seed germination of the present invention, do not need to divide the not easy-operating modes such as cotyledon through mechanical damage removal base portion seed coat, removal unit, only need soak and Sand culture by the Gibberellins solution of suitable concentration, just effectively seed dormancy can be broken, accelerate seed germination and improve germination rate, sprout nearly 30 days in advance than the betel nut Ke seed without any hormone agents process in traditional mode of production, germination rate improves more than 30%.
2, the method for promotion betel nut Ke seed germination of the present invention, betel nut Ke seed is through gibberellin process and bury vernalization with fine sand, and sprout and shorten perdurabgility, germination peak period Relatively centralized, bud seedling high-quality, whole structure is good.
3, the present invention is placed in nursery by being equipped with the husky flowerpot hiding seed, and with covered rearing with plastic film, and moisturizing is carried out in timing watering, allows seed sprout in its natural state, is applicable to producing.
4, the present invention's potassium permanganate carries out disinfection to seed, and with the mode process sand of high-temperature sterilization, effectively prevents betel nut Ke seed to produce and go mouldy, improve the germination rate of betel nut Ke seed
5, the method for promotion betel nut Ke seed germination of the present invention, technological operation is simple, with low cost, handled easily, is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Autumn gathers ripe betel nut Ke seed, is placed in airtight container 2 days.By the betel nut Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean betel nut Ke seed puts into volumetric concentration 0.3% is soaked 40min, then pulls out, dry in the shade after rinsing well with clear water.Betel nut Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir betel nut Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, betel nut Ke seed is pulled out and drain.The Gibberellins solution that betel nut Ke seed after seed soaking puts into 200mg/L is soaked 4d.
The baking oven first river sand cleaned up being placed in 100 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:6 by volume by betel nut Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ carbendazim solutions to sterilization in the flowerpot of breeding bed, every 8d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 2:
Autumn gathers ripe betel nut Ke seed, is placed in airtight container 2 days.By the betel nut Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean betel nut Ke seed puts into volumetric concentration 0.3% is soaked 45min, then pulls out, dry in the shade after rinsing well with clear water.Betel nut Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir betel nut Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, betel nut Ke seed is pulled out and drain.The Gibberellins solution that betel nut Ke seed after seed soaking puts into 300mg/L is soaked 2d.
The baking oven first river sand cleaned up being placed in 120 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:6 by volume by betel nut Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ carbendazim solutions to sterilization in the flowerpot of breeding bed, every 9d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 3:
Autumn gathers ripe betel nut Ke seed, is placed in airtight container 3 days.By the betel nut Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean betel nut Ke seed puts into volumetric concentration 0.3% is soaked 50min, then pulls out, dry in the shade after rinsing well with clear water.Betel nut Ke seed clear water after sterilization is soaked 3d, makes its abundant imbibition; In clear water, fully stir betel nut Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, betel nut Ke seed is pulled out and drain.The Gibberellins solution that betel nut Ke seed after seed soaking puts into 400mg/L is soaked 3d.
The baking oven first river sand cleaned up being placed in 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:6 by volume by betel nut Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ carbendazim solutions to sterilization in the flowerpot of breeding bed, every 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Claims (4)
1. one kind promotes the method for betel nut Ke seed germination, it is characterized in that: comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe betel nut Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe betel nut Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the betel nut Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean betel nut Ke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the betel nut Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir betel nut Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, betel nut Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution betel nut Ke seed after seed soaking being put into 200 ~ 400mg/L soaks 3 ~ 4d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, betel nut Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
2. the method for promotion betel nut Ke seed germination according to claim 1, is characterized in that: the betel nut Ke seed in step (3) and sand are share equally after the ratio of 1:6 is puddled by volume.
3. the method for promotion betel nut Ke seed germination according to claim 1, is characterized in that: the even stand thickness described in step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
4. the method for promotion betel nut Ke seed germination according to claim 1, is characterized in that: the periodically sprinkle bactericide described in step (4) be with volumetric concentration be 1 ‰ carbendazim solutions to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106258989A (en) * | 2016-10-17 | 2017-01-04 | 柳州玲通科技有限责任公司 | A kind of Semen Arecae Ke's tissue cultured seedling root induction method |
CN106417029A (en) * | 2016-10-17 | 2017-02-22 | 柳州三农科技有限公司 | Method for obtaining explant of Lithocarpus areca tissue culture |
CN106417031A (en) * | 2016-10-17 | 2017-02-22 | 柳州玲通科技有限责任公司 | Rootage induction method of lithocarpus amygdalifolius tissue culture seedlings |
CN106472315A (en) * | 2016-10-17 | 2017-03-08 | 柳州玲通科技有限责任公司 | A kind of Semen Arecae Ke's initial culture bud abductive approach |
CN106472313A (en) * | 2016-10-17 | 2017-03-08 | 柳州玲通科技有限责任公司 | A kind of Quercus acutissima tissue cultured seedling root induction method |
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JP2004208545A (en) * | 2002-12-27 | 2004-07-29 | Osaka Gas Co Ltd | Slope-greening method |
CN101715680A (en) * | 2009-11-30 | 2010-06-02 | 宁波市林特科技推广中心 | Method for promoting culture of cyclobalanopsis glauca (Thunb.) Oerst. seedlings |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106258989A (en) * | 2016-10-17 | 2017-01-04 | 柳州玲通科技有限责任公司 | A kind of Semen Arecae Ke's tissue cultured seedling root induction method |
CN106417029A (en) * | 2016-10-17 | 2017-02-22 | 柳州三农科技有限公司 | Method for obtaining explant of Lithocarpus areca tissue culture |
CN106417031A (en) * | 2016-10-17 | 2017-02-22 | 柳州玲通科技有限责任公司 | Rootage induction method of lithocarpus amygdalifolius tissue culture seedlings |
CN106472315A (en) * | 2016-10-17 | 2017-03-08 | 柳州玲通科技有限责任公司 | A kind of Semen Arecae Ke's initial culture bud abductive approach |
CN106472313A (en) * | 2016-10-17 | 2017-03-08 | 柳州玲通科技有限责任公司 | A kind of Quercus acutissima tissue cultured seedling root induction method |
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Application publication date: 20160127 |