CN105325096A - Method for promoting germination of lithocarpus amygdalifolius seeds - Google Patents

Method for promoting germination of lithocarpus amygdalifolius seeds Download PDF

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Publication number
CN105325096A
CN105325096A CN201510739315.2A CN201510739315A CN105325096A CN 105325096 A CN105325096 A CN 105325096A CN 201510739315 A CN201510739315 A CN 201510739315A CN 105325096 A CN105325096 A CN 105325096A
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seed
sand
apricot leaf
germination
seeds
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张莘蔓
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for promoting germination of lithocarpus amygdalifolius seeds. The method is characterized by comprising the procedures of seed collection, seed pretreatment, sand storage for germination acceleration and breeding management and maintenance, the ripe lithocarpus amygdalifolius seeds are collected, selected, disinfected, soaked and subjected to gibberellin treatment, then sand storage for germination acceleration is conducted, and germinated seeds are obtained through breeding management and maintenance. According to the method for promoting germination of the lithocarpus amygdalifolius seeds, seed dormancy can be effectively broken through soaking with plant hormones with the appropriate concentration and sand storage treatment, seed germination is accelerated, the germination rate is raised, and the method is simple in process operation, low in cost, convenient to operate and suitable for popularization and has good economical benefits, social benefits and ecological benefits.

Description

A kind of method promoting apricot leaf Ke seed germination
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method promoting apricot leaf Ke seed germination.
Background technology
Apricot leaf Ke ( lithocarpusamygdalifolius (Skan) Hayata) have another name called apricot leaf lithocarpus glaber, red C.-glauca, ridge plum, be Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, the shoot that spring and summer extracts out and the tender leaf back side is close is rolled up pubescence by yellowish-brown, hair all comes off after autumn.The life of tassel locusta list fringe armpit or many fringes line up panicle, and rhachis is close by pubescence; Every 3 clusters of female flower, sometimes have concurrently single scattered.The nearly spheroidal of acorn-cup, full bag nut, in discontinuous ring-type; The fruit wall of nut is slightly thicker than acorn-cup wall, and nut top is by micro-pubescence.Be distributed in Central Taiwan and south, south Fujian, the southeast, Guangdong be about tropic of cancer areas to the south, Hainan.Being born in height above sea level 1100-2300 rice evergreen broad-leaved forest or needle, Broad-leaved Mixed Forest, is often upper canopy species.
At present, apricot leaf Ke resource is still in wild distribution, but along with keeping the demand of Plant Diversity, large-scale artificial cultivation apricot leaf Ke resource is imperative.Because apricot leaf Ke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And the apricot leaf Ke percentage of seedgermination without any hormone agents process in traditional mode of production often only has about 50%, and germinating time evening, emerge irregular, limit the large-scale planting of apricot leaf Ke.
Summary of the invention
Object of the present invention is the deficiency overcoming existing apricot leaf Ke Seed germination techniques, provides a kind of germination rate and the quickening germination physiology that can improve apricot leaf Ke seed, workable, practical simplicity, the method for promotion apricot leaf Ke seed germination that cost is low.
Technical scheme of the present invention is as follows:
A kind of method promoting apricot leaf Ke seed germination, comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe apricot leaf Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe apricot leaf Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the apricot leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean apricot leaf Ke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the apricot leaf Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir apricot leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, apricot leaf Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution apricot leaf Ke seed after seed soaking being put into 400 ~ 600mg/L soaks 2 ~ 3d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, apricot leaf Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
Preferably, the apricot leaf Ke seed in above step (3) and sand are share equally after the ratio of 1:5 is puddled by volume.
Preferably, the even stand thickness described in above step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
Preferably, the periodically sprinkle bactericide described in above step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
The present invention relative to prior art, the advantage had and good effect as follows:
1, the method for promotion apricot leaf Ke seed germination of the present invention, do not need to divide the not easy-operating modes such as cotyledon through mechanical damage removal base portion seed coat, removal unit, only need soak and Sand culture by the Gibberellins solution of suitable concentration, just effectively seed dormancy can be broken, accelerate seed germination and improve germination rate, sprout nearly 20 days in advance than the apricot leaf Ke seed without any hormone agents process in traditional mode of production, germination rate improves more than 30%.
2, the method for promotion apricot leaf Ke seed germination of the present invention, apricot leaf Ke seed is through gibberellin process and bury vernalization with fine sand, and sprout and shorten perdurabgility, germination peak period Relatively centralized, bud seedling high-quality, whole structure is good.
3, the present invention is placed in nursery by being equipped with the husky flowerpot hiding seed, and with covered rearing with plastic film, and moisturizing is carried out in timing watering, allows seed sprout in its natural state, is applicable to producing.
4, the present invention's potassium permanganate carries out disinfection to seed, and with the mode process sand of high-temperature sterilization, effectively prevents apricot leaf Ke seed to produce and go mouldy, improve the germination rate of apricot leaf Ke seed
5, the method for promotion apricot leaf Ke seed germination of the present invention, technological operation is simple, with low cost, handled easily, is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Autumn gathers ripe apricot leaf Ke seed, is placed in airtight container 2 days.By the apricot leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean apricot leaf Ke seed puts into volumetric concentration 0.3% is soaked 40min, then pulls out, dry in the shade after rinsing well with clear water.Apricot leaf Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir apricot leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, apricot leaf Ke seed is pulled out and drain.The Gibberellins solution that apricot leaf Ke seed after seed soaking puts into 400mg/L is soaked 3d.
The baking oven first river sand cleaned up being placed in 100 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by apricot leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 2:
Autumn gathers ripe apricot leaf Ke seed, is placed in airtight container 2 days.By the apricot leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean apricot leaf Ke seed puts into volumetric concentration 0.3% is soaked 45min, then pulls out, dry in the shade after rinsing well with clear water.Apricot leaf Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir apricot leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, apricot leaf Ke seed is pulled out and drain.The Gibberellins solution that apricot leaf Ke seed after seed soaking puts into 500mg/L is soaked 2d.
The baking oven first river sand cleaned up being placed in 120 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by apricot leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 9d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 3:
Autumn gathers ripe apricot leaf Ke seed, is placed in airtight container 3 days.By the apricot leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean apricot leaf Ke seed puts into volumetric concentration 0.3% is soaked 50min, then pulls out, dry in the shade after rinsing well with clear water.Apricot leaf Ke seed clear water after sterilization is soaked 3d, makes its abundant imbibition; In clear water, fully stir apricot leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, apricot leaf Ke seed is pulled out and drain.The Gibberellins solution that apricot leaf Ke seed after seed soaking puts into 600mg/L is soaked 2d.
The baking oven first river sand cleaned up being placed in 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by apricot leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.

Claims (4)

1. one kind promotes the method for apricot leaf Ke seed germination, it is characterized in that: comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe apricot leaf Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe apricot leaf Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the apricot leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean apricot leaf Ke seed being put into volumetric concentration 0.3% soaks 40 ~ 50min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the apricot leaf Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir apricot leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, apricot leaf Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution apricot leaf Ke seed after seed soaking being put into 400 ~ 600mg/L soaks 2 ~ 3d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, apricot leaf Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
2. a kind of method promoting apricot leaf Ke seed germination according to claim 1, is characterized in that: the apricot leaf Ke seed in step (3) and sand are share equally after the ratio of 1:5 is puddled by volume.
3. a kind of method promoting apricot leaf Ke seed germination according to claim 1, is characterized in that: the even stand thickness described in step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
4. a kind of method promoting apricot leaf Ke seed germination according to claim 1, it is characterized in that: the periodically sprinkle bactericide described in step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
CN201510739315.2A 2015-11-04 2015-11-04 Method for promoting germination of lithocarpus amygdalifolius seeds Pending CN105325096A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106416911A (en) * 2016-10-17 2017-02-22 柳州三农科技有限公司 Lithocarpus amygdalifolius tissue culture explant obtaining method
CN110933969A (en) * 2019-11-08 2020-03-31 广西南亚热带农业科学研究所 Method for improving germination rate of litsea cubeba seeds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09172819A (en) * 1995-12-22 1997-07-08 Toko Kensetsu Kk Woody plant community-establishing work on face of slope
CN103766118A (en) * 2014-01-15 2014-05-07 湖南省森林植物园 Storing method and breeding method of lithocarpus amygdalifolius seeds
CN103988607A (en) * 2014-05-23 2014-08-20 广西壮族自治区林业科学研究院 Method for promoting germination of lithocarpus polystachyus rehd seeds
CN104081980A (en) * 2014-07-03 2014-10-08 广西壮族自治区林业科学研究院 Method for lithocarpus polysachyus rehd container seedling culturing

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09172819A (en) * 1995-12-22 1997-07-08 Toko Kensetsu Kk Woody plant community-establishing work on face of slope
CN103766118A (en) * 2014-01-15 2014-05-07 湖南省森林植物园 Storing method and breeding method of lithocarpus amygdalifolius seeds
CN103988607A (en) * 2014-05-23 2014-08-20 广西壮族自治区林业科学研究院 Method for promoting germination of lithocarpus polystachyus rehd seeds
CN104081980A (en) * 2014-07-03 2014-10-08 广西壮族自治区林业科学研究院 Method for lithocarpus polysachyus rehd container seedling culturing

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106416911A (en) * 2016-10-17 2017-02-22 柳州三农科技有限公司 Lithocarpus amygdalifolius tissue culture explant obtaining method
CN110933969A (en) * 2019-11-08 2020-03-31 广西南亚热带农业科学研究所 Method for improving germination rate of litsea cubeba seeds

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