CN102972191A - Method for breeding and cultivating taxus mairei with seed - Google Patents
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Abstract
The invention provides a method for breeding and cultivating taxus mairei with a seed, and the method comprises the following steps that a taxus mairei seedling is obtained by breeding and cultivating the sexual seed; the taxus mairei seedling is transplanted and planted, and a large taxus mairei seedling and a young tree are obtained; and the cultivation area of the taxus mairei is expanded. Special cultivation management measures for the taxus mairei are adopted to establish a taxus mairei breeding and cultivation technical system, a manual way to breed the taxus mairei is opened up, so that the large-scale industrial production of the taxus mairei becomes possible, the market demand for the taxus mairei is met, and a good test system is provided for protecting endangered species of wild plant resources.
Description
Technical field
The invention belongs to the propagation and cultivation technology of southern enqlish yew, a kind of propagation and cultivation method of southern enqlish yew specifically is provided.
Background technology
Chinese yew has another name called Japanese yew, is the quaternary glacier Relict Plant, is called as " living fossil " in the vegetable kingdom, because of its resource scarcity, is listed in world's rare tree species and is protected.As the main separation and Extraction from the wild resource bark of Ramulus et folium taxi cuspidatae of the taxol of the whole world one of three large cancer therapy drugs, and the Chinese yew of taking the age of tree more than 30 years just has exploitation value.At present; also quite large to its demand in the world, cause cutting down in a large number the large tree of Chinese yew and from bark, extract taxol, this species conservation and area distribution to Chinese yew genus plants brings threat; destroy the most at last ecotope, cause the exhaustion of wild resource and the extinction of species.It is lower to breed the Chinese yew survival rate with usual manner, has now become extinct specie in imminent danger.
Having now and breeding southern enqlish yew generally is cottage propagation, and is difficult to behind the tloig-cutlage in the reproductive process take root, and survival rate is very low, and not improvement of kind, just simply copies breeding.
Summary of the invention
For the deficiencies in the prior art, the invention provides another kind of propagation method, the method is by sexual seed seedling-raising breeding breeding, obtains the Chinese yew seedling, obtains Chinese yew seedlings, sapling thicket behind transplant planting again, enlarges the southern enqlish yew area under cultivation.The method can improve the Chinese yew kind.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is:
A kind of method with seminal propagation and cultivation southern enqlish yew, the method comprises:
Nursery is selected: the Schattenseite that the selection gradient is 5 °～15 ° or half Schattenseite are as nursery;
Make bed: adopt high bed to grow seedlings, the hypothallus in seedbed is by forming earth and husky the mixing, and wherein the quality percentage composition of earth accounts for 70%-80% wholely; The medium of seedling bed layer thickness is 5 cm～10 cm; 50kg～100kg superphosphate, 25 kg～50kg urea and ferrous sulfate 5 kg～6kg are used in every mu of seedbed;
Seed collection:
The erythrocarpus of gathering for 9～November removes red pericarp layer and pulp layer, cleans, and be 0.3%～0.6% the liquor potassic permanganate 1.5h-2.5h that soaks seed with mass concentration then, drain;
Seed storage:
The employing mode of storing sth. in a cellar is preserved, and the seed storage phase is 15～18 months; Spread first the thick sand bed of one deck 8cm～12cm, then lay the Seed Layer of one deck 1cm～2cm thickness above sand bed, cover the sand bed of one deck 3cm～5cm thickness again, according to said method circulation is laid, at last the sand bed of the thick 5cm of lid layer～6 cm above seed; First month control water consumption of sand bed is 18%～22%, second month control water consumption of sand bed is 12%～15%, the control water consumption of sand bed was 10%～12% in three month, the control water consumption of sand bed is controlled at 6%～8% in several months afterwards, until sowing control in front the 2nd month water consumption of sand bed is 28%～32%, sowing front the 1st month control water consumption of sand bed is 36%～40%;
Seed soaking:
Mix 1:1～1.1 by volume with 40 °～55 ° liquor and 35 ℃-55 ℃ water first before the sowing, soak seed 20 min～30min, then be 0.5%～0.6% gibberellin aqueous solution soaking 20 h～24h with mass concentration, induce the generation of hydrolase, short its sprouted the seed after obtaining soaking;
Seedtime:
Take spring sowing as main, 4～May surface air temperature rise to 15 ℃ with upper seeding wheel; On the seedbed, covering a layer thickness is earth or the sand of 1cm～1.5cm with the seed broadcasting after soaking; The spray body volume concentrations is that 0.1%～0.2% liquor potassic permanganate and volumetric concentration are 1%～2% copperas solution behind the earthing, every mu of seedbed is sprayed 60 kilograms～80 kilograms, hide at 30cm above the face of seedbed～50cm place with shade net again, keep bed surface dark and damp;
Rich water quality management:
Before emerging and seedling growth stage maintenance matrix top layer is moistening, guarantees the required moisture of seedling growth; The Chinese yew seedling from occur primary leaf enter the fast-growing phase begin the fertilising; Fertilising 6-7 month is main; August nursery stock advance the people grow the later stage stop the fertilising;
Prevention and elimination of disease and pests:
The nursery stock bordeaux mixture that rear sprinkling mass ratio is 1:120～150 that all sprouts, the prevention damping-off of larch Seedlings sprayed 1 time every 1 week; Each every mu of seedbed is sprayed 80～100 kilograms;
Transplant planting
When seedling grew to 20cm～30cm, seeding row spacing was transplanted by (50～60) cm * (50～60) cm; Seedling is long to carry out the transplanting second time by (2.5～3) m * (2.5～3) m during to 1m～1.5m; Strengthen shading, watering in 1 week after transplanting, carry out weeding according to the growth of seedling situation after the week;
Tending [after young plantation:
Annual 2 times of tending [after young plantation, for several years running, till the young growth closing; Annual fertilising in front 5 years is 1 time during the tending [after young plantation, and in mass ratio fertile 0.2 kg of mixing that forms of 1:1～1.2～0.3kg of urea and superphosphate is executed in every strain, promotes sapling up-growth.
Described earth is preferably humus soil or vegetable garden soil; Described sand refers to that preferably particle diameter is that 100 orders-300 purpose is husky.
Step (1) nursery is selected to be preferably: be Schattenseite or the mild mountain region of half Schattenseite of selecting 5 °～15 ° of the gradients, the soil liquid manure condition is good, and convenient transportation is had abundant water resources, and the place of being convenient to manage is as nursery.
Step (2) is described to be made bed wholely and is preferably, and adopts high bed to grow seedlings, and the long lO～30m of bed surface, wide 1.2～1.5m, is higher than pavement 20～30cm, pavement 30～50cm.The bed surface leveling is steady and sure, and the direction of bed is advisable with the north-south.Drainage trenching around the nursery accomplishes that interior water is not long-pending, and outer water does not flood.The seedbed is mixed into preferably matrix soil of short texture, ventilative and preserve moisture and fertility performance, dark 5～10 cm of medium of seedling bed with vegetable garden soil or humus soil 70～80% and river sand 20～30%.In order to increase the matrix fertility, use 50～100kg superphosphate and 25～50kg urea in conjunction with ploughing every mu; In order to prevent nursery stock generation damage by disease and insect, every mu of seedbed soil table sprays ferrous sulfate 5～6kg.
The described seed collection of step (3) is preferably, and is the erythrocarpus of gathering annual 9～November, and Best Times is 2 weeks after the Frost's Descent; Repeatedly rub at coarse concrete floor with sole or damaged tire, rub with the hands and remove red pseudocarp skin and pulp, its hard kind shell of wear down is sure not to grind mashed kind of benevolence.Make seed permeable, ventilative easily.With 0.5% the liquor potassic permanganate 2h that soaks seed, drain away the water after cleaning.
The described seed storage of step (4) is preferably, employing is stored sth. in a cellar, spread first the thick clean river sand of one deck 8～12cm, husky humidity is pinched and can agglomeratingly be advisable with hand, then shop one deck seed on sand, thickness 1～2 cm, lid layer sand 3～5 cm again spread one deck seed by this circulation, lid layer is husky, at last the sand of thick 5～6 cm of lid layer above the seed face.The first month water consumption of sand bed is controlled at 18～22%, the second month water consumption of sand bed is controlled at 12%～15%, water consumption of sand bed was controlled at 10%～12% in three month, be controlled at later on 6%～8%, sow front 2 months water consumption of sand beds and be controlled at 28～32%, sow front 1 month water consumption of sand bed and be controlled at 36～40%; The seed storage phase is 15～18 months, and germination rate can reach 90%～98%.Check the humidity of 1 sand and the germplasm situation of seed every month, during inspection seed is sifted out from sand, as then in time the water spray benefit is wet to find drying, mildew seed is rejected.
The described seed soaking of step (5) is preferably, before the sowing first the warm water with 5O degree liquor and 40 ℃ by volume mix 1:1～1.1, seed soaking 20～30min is Gibberellins solution immersion 20～24h of 0.5～0.6% again with mass concentration after pulling out, can induce the generation of hydrolase, short its sprouting.
It is main that the described seedtime of step (6) is preferably to sow in spring, early unsuitable evening.4～May surface air temperature rise to 15 ℃ with upper seeding wheel.The seed broadcasting handled well on the seedling stage bed, is covered fine earth or the river sand of 1～1.5cm.The spray body volume concentrations is that 0.1%～0.2% liquor potassic permanganate and volumetric concentration are 1%～2% copperas solution behind the earthing, and every mu of seedbed is sprayed 60 kilograms～80 kilograms, hides from seedbed face 30～50cm with shade net again, keeps bed surface dark and damp.
The described rich water quality management of step (7) is preferably, and will keep the matrix top layer moistening with seedling growth stage before emerging, and guarantees the required moisture of seedling growth.It is an amount of to water, and few many water spray means of employing amount both can reduce surface temperature, can regulate again nursery stock relative moisture on every side.To control to the seedling growth later stage and to water, will water water before surviving the winter 1 time, improve the cold tolerance of seedling.The Chinese yew seedling enters the fast-growing phase and begins to topdress from primary leaf occurring.Topdress and to combine with water spray, executing foliage fertilizer as main, urea and water are dissolved in the water and spray or spill in 1:100～150 in mass ratio, execute 150～200 kilograms for each every mu, in case fertilizer damage occurs; June is take nitrogen fertilizer application as main, and spilling 1 inferior quality concentration every two weeks is 1%～2% urea liquid, applies fertilizer altogether 2 times, executes 200～300 kilograms for each every mu; July is take potassium application as main, and spilling 1 inferior quality concentration every first quarter moon is 0.2%～0.3% phosphoric acid dioxy potassium, applies fertilizer altogether 2 times, executes 250～300 kilograms for each every mu; Nursery stock advanced the people and later stage of growing stopped fertilising August, promoted the abundant lignification of nursery stock, was convenient to the nursery stock safe overwintering.Seedling Stage is wanted timely weeding, accomplishes bed surface and pavement in will to prevent from becoming flexible shoot root during weeding without weeds.
The described prevention and elimination of disease and pests of step (8) is preferably, the nursery stock bordeaux mixture that rear sprinkling mass ratio is 1:120～150 that all sprouts, and the prevention damping-off of larch Seedlings sprayed 1 time every 1 week; Each every mu of seedbed is sprayed 80～100 kilograms; Mainly be grub, mole cricket, cutworm harm seedling, spray mass ratio when insect pest occurs and be 5～8% dichlorvos, Kothrin 1～2 time, 100～120 kg is sprayed in each every mu of seedbed.
The described transplant planting of step (9) when seedling grows to 20～30cm, is selected the middle and lower part of thicker soil, waste mountain waste that moisture condition is good, and seeding row spacing is transplanted by 50～60 * 50～60cm.Carrying out the second time by 2.5～3m * 2.5～3m during seedling length to 1～1.5m transplants.To strengthen shading, watering in 1 week after transplanting, carry out weeding according to the growth of seedling situation later on.
The described tending [after young plantation of step (10), annual 2 times, for several years running, till the young growth closing.The in mass ratio mixing fertilizer 0.2～0.3kg of 1:1～1.2 of urea and superphosphate is executed in annual fertilising in front 5 years 1 time, every strain, promotes sapling up-growth.
Compared with prior art, advantage of the present invention is:
The present invention obtains the Chinese yew seedling by sexual seed seedling-raising breeding breeding, obtains Chinese yew seedlings, sapling thicket behind transplant planting again, enlarges the southern enqlish yew area under cultivation.Adopt the proprietary cultivation management measure of southern enqlish yew; set up southern enqlish yew breeding and transplantation technique system; opened up the approach of an artificial propagation southern enqlish yew; make the production of southern enqlish yew large-scale industrial become possibility; thereby satisfying the market is to the needs of southern enqlish yew, for the protection of wild plant resource in imminent danger provides good test system.The method is sexual propagation, can improve the Chinese yew kind, improves survival rate.
Embodiment
Further explain and illustrate technical scheme of the present invention below in conjunction with embodiment.
Embodiment
Southern enqlish yew can carry out sexual propagation by seed seedling-raising, enlarges area under cultivation.
1, nursery is selected
Select Schattenseite or the mild mountain region of half Schattenseite of 5 °～15 ° of the gradients, the soil liquid manure condition is good, and convenient transportation is had abundant water resources, and the place of being convenient to manage is as nursery.
2, make bed wholely
Adopt high bed to grow seedlings, the long lO～30m of bed surface, wide 1.2～1.5m, be higher than pavement 20～30cm, pavement 30～50cm.The bed surface leveling is steady and sure, and the direction of bed is advisable with the north-south.Drainage trenching around the nursery accomplishes that interior water is not long-pending, and outer water does not flood.The seedbed is mixed into preferably matrix soil of short texture, ventilative and preserve moisture and fertility performance, dark 5～10 cm of medium of seedling bed with vegetable garden soil or humus soil 70～80% and husky 20～30%.In order to increase the matrix fertility, use 50～100kg superphosphate and 25～50kg urea in conjunction with ploughing every mu; In order to prevent nursery stock generation damage by disease and insect, every mu of seedbed soil table sprays ferrous sulfate 5～6kg.
3, seed collection
The erythrocarpus of gathering for 9～November, Best Times is 2 weeks after the Frost's Descent; Repeatedly rub at coarse concrete floor with sole or damaged tire, rub with the hands and remove red pseudocarp skin and pulp, its hard kind shell of wear down is sure not to grind mashed kind of benevolence, makes seed permeable, ventilative easily.After cleaning with 0.5% the liquor potassic permanganate 2h that soaks seed, drain well.
4, seed storage
Taxus chinensis var. mairei Seeds adopts the mode of storing sth. in a cellar, spread first the thick clean river sand of one deck 8～12cm, husky humidity is pinched and can agglomeratingly be advisable with hand, then shop one deck seed on sand, thickness 1～2cm, lid layer sand 3～5cm again, spread one deck seed by this circulation, lid layer is husky, at last the sand of thick 5～6 cm of lid layer above the seed face.The first month water consumption of sand bed is controlled at 18～22%, the second month water consumption of sand bed is controlled at 12%～15%, water consumption of sand bed was controlled at 10%～12% in three month, be controlled at later on 6%～8%, sow front 2 months water consumption of sand beds and be controlled at 28～32%, sow front 1 month water consumption of sand bed and be controlled at 36～40%; The seed storage phase is 15～18 months, and germination rate can reach 90%～98%.Check the humidity of 1 sand and the germplasm situation of seed every month, during inspection seed is sifted out from sand, as then in time the water spray benefit is wet to find drying, mildew seed is rejected.
5, seed soaking
Before the sowing first the warm water with 5O degree liquor and 40 ℃ by volume mix 1:1～1.1, seed soaking 20～30min is that 0.5～0.6% Gibberellins solution is soaked 20～24h with mass concentration again after pulling out, can induce the generation of hydrolase, urgees its sprouting.
6, seedtime
Take spring sowing as main, should be early unsuitable late.4～May surface air temperature rise to 15 ℃ with upper seeding wheel.The seed broadcasting handled well on the seedling stage bed, is covered fine earth or the river sand of 1～1.5cm.The spray body volume concentrations is that 0.1%～0.2% liquor potassic permanganate and volumetric concentration are 1%～2% copperas solution behind the earthing, and every mu of seedbed is sprayed 60 kilograms～80 kilograms, hides from seedbed face 30～50cm with shade net again, keeps bed surface dark and damp.
7, rich water quality management
To keep the matrix top layer moistening with seedling growth stage before emerging, guarantee the required moisture of seedling growth.It is an amount of to water, and few many water spray means of employing amount both can reduce surface temperature, can regulate again nursery stock relative moisture on every side.To control to the seedling growth later stage and to water, will water water before surviving the winter 1 time, improve the cold tolerance of seedling.The Chinese yew seedling enters the fast-growing phase and begins to topdress from primary leaf occurring.Topdress and to combine with water spray, executing foliage fertilizer as main, urea and water are dissolved in the water and spray or spill in 1:100～150 in mass ratio, execute 150～200 kilograms for each every mu, in case fertilizer damage occurs; June is take nitrogen fertilizer application as main, and spilling 1 inferior quality concentration every two weeks is 1%～2% urea liquid, applies fertilizer altogether 2 times, executes 200～300 kilograms for each every mu; July is take potassium application as main, and spilling 1 inferior quality concentration every first quarter moon is 0.2%～0.3% phosphoric acid dioxy potassium, applies fertilizer altogether 2 times, executes 250～300 kilograms for each every mu; Nursery stock advanced the people and later stage of growing stopped fertilising August, promoted the abundant lignification of nursery stock, was convenient to the nursery stock safe overwintering.Seedling Stage is wanted timely weeding, accomplishes bed surface and pavement in will to prevent from becoming flexible shoot root during weeding without weeds.
8, prevention and elimination of disease and pests
The nursery stock bordeaux mixture that rear sprinkling mass ratio is 1:120～150 that all sprouts, the prevention damping-off of larch Seedlings sprayed 1 time every 1 week; Each every mu of seedbed is sprayed 80～100 kilograms; Mainly be grub, mole cricket, cutworm harm seedling, spray mass ratio when insect pest occurs and be 5～8% dichlorvos, Kothrin 1～2 time, 100～120 kg is sprayed in each every mu of seedbed.9, transplant planting
When seedling grows to 20～30cm, select the middle and lower part of thicker soil, waste mountain waste that moisture condition is good, seeding row spacing is transplanted by 50～60 * 50～60cm.Carrying out the second time by 2.5～3m * 2.5～3m during seedling length to 1～1.5m transplants.To strengthen shading, watering in 1 week after transplanting, carry out weeding according to the growth of seedling situation later on.
10, tending [after young plantation
Annual 2 times of tending [after young plantation, for several years running, till the young growth closing.The in mass ratio mixing fertilizer 0.2～0.3kg of 1:1～1.2 of urea and superphosphate is executed in annual fertilising in front 5 years 1 time, every strain, promotes sapling up-growth.
Utilize male and female to be pollinated to intersect and form seed and raise up seed, not only have a large amount of seeds to produce and to breed more new talent, can also bring up good filial generation, improve the pure content of southern enqlish yew branches and leaves, bark Japanese yew.The new individuality that asexual process produces is fully the same and unchanged with parent, can not put forward pure content, Resistant, drought-enduring, cold-resistant and Fast Growth ability.
Claims (5)
- One kind with seminal propagation and the cultivation southern enqlish yew method, it is characterized in that the method comprises:Nursery is selected: the Schattenseite that the selection gradient is 5 °～15 ° or half Schattenseite are as nursery;Make bed: adopt high bed to grow seedlings, the hypothallus in seedbed is by forming earth and husky the mixing, and wherein the quality percentage composition of earth accounts for 70%-80% wholely; The medium of seedling bed layer thickness is 5 cm～10 cm; 50kg～100kg superphosphate, 25 kg～50kg urea and ferrous sulfate 5 kg～6kg are used in every mu of seedbed;Seed collection:The erythrocarpus of gathering for 9～November removes red pericarp layer and pulp layer, cleans, and be 0.3%～0.6% the liquor potassic permanganate 1.5h-2.5h that soaks seed with mass concentration then, drain;Seed storage:The employing mode of storing sth. in a cellar is preserved, and the seed storage phase is 15～18 months; Spread first the thick sand bed of one deck 8cm～12cm, then lay the Seed Layer of one deck 1cm～2cm thickness above sand bed, cover the sand bed of one deck 3cm～5cm thickness again, according to said method circulation is laid, at last the sand bed of the thick 5cm of lid layer～6 cm above seed; First month control water consumption of sand bed is 18%～22%, second month control water consumption of sand bed is 12%～15%, the control water consumption of sand bed was 10%～12% in three month, the control water consumption of sand bed is controlled at 6%～8% in several months afterwards, until sowing control in front the 2nd month water consumption of sand bed is 28%～32%, sowing front the 1st month control water consumption of sand bed is 36%～40%;Seed soaking:Mix 1:1～1.1 by volume with 40 °～55 ° liquor and 35 ℃-55 ℃ water first before the sowing, soak seed 20 min～30min, then be 0.5%～0.6% gibberellin aqueous solution soaking 20 h～24h with mass concentration, induce the generation of hydrolase, short its sprouted the seed after obtaining soaking;Seedtime:Take spring sowing as main, 4～May surface air temperature rise to 15 ℃ with upper seeding wheel; On the seedbed, covering a layer thickness is earth or the sand of 1cm～1.5cm with the seed broadcasting after soaking; The spray body volume concentrations is that 0.1%～0.2% liquor potassic permanganate and volumetric concentration are 1%～2% copperas solution behind the earthing, every mu of seedbed is sprayed 60 kilograms～80 kilograms, hide at 30cm above the face of seedbed～50cm place with shade net again, keep bed surface dark and damp;Rich water quality management:Before emerging and seedling growth stage maintenance matrix top layer is moistening, guarantees the required moisture of seedling growth; The Chinese yew seedling from occur primary leaf enter the fast-growing phase begin the fertilising; Fertilising 6-7 month is main; August nursery stock advance the people grow the later stage stop the fertilising;Prevention and elimination of disease and pests:The nursery stock bordeaux mixture that rear sprinkling mass ratio is 1:120～150 that all sprouts, the prevention damping-off of larch Seedlings sprayed 1 time every 1 week; Each every mu of seedbed is sprayed 80～100 kilograms;Transplant planting:When seedling grew to 20cm～30cm, seeding row spacing was transplanted by (50～60) cm * (50～60) cm; Seedling is long to carry out the transplanting second time by (2.5～3) m * (2.5～3) m during to 1m～1.5m; Strengthen shading, watering in 1 week after transplanting, carry out weeding according to the growth of seedling situation after the week;Tending [after young plantation:Annual 2 times of tending [after young plantation, for several years running, till the young growth closing; Annual fertilising in front 5 years is 1 time during the tending [after young plantation, and in mass ratio fertile 0.2 kg of mixing that forms of 1:1～1.2～0.3kg of urea and superphosphate is executed in every strain, promotes sapling up-growth.
- 2. described with the method for seminal propagation with the cultivation southern enqlish yew according to claim 1, it is characterized in that described earth is humus soil or vegetable garden soil; Described sand refers to that particle diameter is that 100 orders-300 purpose is husky.
- 3. described method with seminal propagation and cultivation southern enqlish yew according to claim 1 and 2 is characterized in that the time of the described seed collection of step (3) is after the Frost's Descent in 2 weeks.
- 4. described method with seminal propagation and cultivation southern enqlish yew according to claim 1 and 2 is characterized in that, in the described rich water quality management of step (7): water to seedling growth later stage control, water and adopt on a small quantity repeatedly water spray means; Water water before surviving the winter 1 time, improve the cold tolerance of seedling; The Chinese yew seedling enters the fast-growing phase and begins to topdress from primary leaf occurring, topdress and to combine with water spray, executing foliage fertilizer as main, urea and water are dissolved in the water and spray or spill in 1:100～150 in mass ratio, execute 150～200 kilograms for each every mu, in case fertilizer damage occurs; June is take nitrogen fertilizer application as main, and spilling 1 inferior quality concentration every two weeks is 1%～2% urea liquid, applies fertilizer altogether 2 times, executes 200～300 kilograms for each every mu; July is take potassium application as main, and spilling 1 inferior quality concentration every first quarter moon is 0.2%～0.3% phosphoric acid dioxy potassium, applies fertilizer altogether 2 times, executes 250～300 kilograms for each every mu; Nursery stock advanced the people and later stage of growing stopped fertilising August, promoted the abundant lignification of nursery stock, was convenient to the nursery stock safe overwintering.
- 5. described with the method for seminal propagation with the cultivation southern enqlish yew according to claim 1 and 2, it is characterized in that, the described prevention and elimination of disease and pests of step (8) is sprayed mass ratio and is 5%～8% dichlorvos or Kothrin 1～2 time when insect pest occurs, each every mu is sprayed 100～120 kg.
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| CN107896770A (en) * | 2017-11-01 | 2018-04-13 | 柳州市朴鲁渔耕生态种养专业合作社 | A kind of method for culturing seedlings of Chinese yew |
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| CN103444393A (en) * | 2013-08-14 | 2013-12-18 | 贵州黔城晟睿林业科技发展有限公司 | Method for breeding Chinese yew seeds |
| CN103430737A (en) * | 2013-08-14 | 2013-12-11 | 江苏鼎钰生态农业科技有限公司 | Chinese yew planting method |
| CN104322229A (en) * | 2013-11-30 | 2015-02-04 | 黎观祥 | Cultivation method of Chinese yew |
| CN103636393A (en) * | 2013-12-16 | 2014-03-19 | 周丽连 | Cultivation method of taxus chinensis |
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| CN106105992A (en) * | 2016-07-19 | 2016-11-16 | 泸州千秋辉煌农业有限公司 | A kind of method of Ramulus et folium taxi cuspidatae seminal propagation |
| CN106386113A (en) * | 2016-08-31 | 2017-02-15 | 江苏红豆杉生物科技股份有限公司 | Container seedling raising method for Taxus mairei |
| CN106508580A (en) * | 2016-11-11 | 2017-03-22 | 江西天海科技发展集团有限公司 | Rubus-chingii seed breeding method |
| CN107896770A (en) * | 2017-11-01 | 2018-04-13 | 柳州市朴鲁渔耕生态种养专业合作社 | A kind of method for culturing seedlings of Chinese yew |
| CN109429614A (en) * | 2018-11-16 | 2019-03-08 | 马步华 | A method of improving Taxus chinensis var. mairei Seeds vigor |
| CN110249889A (en) * | 2019-07-09 | 2019-09-20 | 宁乡县仁华农业发展有限责任公司 | A kind of artificial method for planting of Chinese yew |
| CN111602550A (en) * | 2020-03-25 | 2020-09-01 | 普洱大华林业科技有限公司 | Artificial seedling raising method using taxus chinensis seeds |
| CN112616536A (en) * | 2020-12-09 | 2021-04-09 | 中国林业科学研究院亚热带林业实验中心 | Wild-imitating method for artificially improving seed germination rate of natural taxus mairei population and promoting seedling renewal |
| CN112616537A (en) * | 2020-12-09 | 2021-04-09 | 中国林业科学研究院亚热带林业实验中心 | Method for improving survival rate of sprouting seedlings in natural population of taxus mairei |
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