CN108893466B - 测序接头、测序接头组和超低频突变的检测方法 - Google Patents
测序接头、测序接头组和超低频突变的检测方法 Download PDFInfo
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Abstract
Description
试剂名称 | 体积 |
水 | 8μl |
KAPA End Repair Buffer(10X) | 7μl |
KAPA End Repair Enzyme Mix | 5μl |
总体积 | 20μl |
试剂名称 | 1个样本 | 8个样本 | 48个样本 |
KAPA A-Tailing Buffer(10X) | 5μl | 40μl | 240μl |
KAPA A-Tailing Enzyme | 3μl | 24μl | 144μl |
总体积 | 8μl | 64μl | 384μl |
名称 | 体积 |
Cot-1DNA | 5μl |
xGen Universal Blocking Oligo P5 | 1μl |
xGen Universal Blocking Oligo P7(6nt) | 1μl |
试剂名称 | 1个反应体积 |
xGen 2X Hybridization Buffer | 8.5μL |
xGen Hybridization Buffer Enhancer | 2.7μL |
Nuclease-Free Water | 1.8μL |
总体积 | 13μL |
试剂名称 | 1×工作液体积 | 1×工作液储存温度 |
Wash Buffer I | 100μL | 65℃ |
Wash Buffer I | 200μL | 室温(15-25℃) |
Stringent Wash Buffer | 400μL | 65℃ |
试剂名称 | 基因组文库 |
2×KAPA HiFi HotStart ReadyMix | 25μL |
10uM P5/P7primer | 2μL |
总体积 | 27μL |
Claims (6)
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CN201810564438.0A CN108893466B (zh) | 2018-06-04 | 2018-06-04 | 测序接头、测序接头组和超低频突变的检测方法 |
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