CN108893466A - 测序接头、测序接头组和超低频突变的检测方法 - Google Patents
测序接头、测序接头组和超低频突变的检测方法 Download PDFInfo
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Abstract
Description
试剂名称 | 体积 |
水 | 8μl |
KAPA End Repair Buffer(10X) | 7μl |
KAPA End Repair Enzyme Mix | 5μl |
总体积 | 20μl |
试剂名称 | 1个样本 | 8个样本 | 48个样本 |
KAPA A-Tailing Buffer(10X) | 5μl | 40μl | 240μl |
KAPA A-Tailing Enzyme | 3μl | 24μl | 144μl |
总体积 | 8μl | 64μl | 384μl |
名称 | 体积 |
Cot-1DNA | 5μl |
xGen Universal Blocking Oligo P5 | 1μl |
xGen Universal Blocking Oligo P7(6nt) | 1μl |
试剂名称 | 1个反应体积 |
xGen 2X Hybridization Buffer | 8.5μL |
xGen Hybridization Buffer Enhancer | 2.7μL |
Nuclease-Free Water | 1.8μL |
总体积 | 13μL |
试剂名称 | 1×工作液体积 | 1×工作液储存温度 |
Wash Buffer I | 100μL | 65℃ |
Wash Buffer I | 200μL | 室温(15-25℃) |
Stringent Wash Buffer | 400μL | 65℃ |
试剂名称 | 基因组文库 |
2×KAPA HiFi HotStart ReadyMix | 25μL |
10uM P5/P7primer | 2μL |
总体积 | 27μL |
Claims (16)
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CN201810564438.0A CN108893466B (zh) | 2018-06-04 | 2018-06-04 | 测序接头、测序接头组和超低频突变的检测方法 |
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CN201810564438.0A CN108893466B (zh) | 2018-06-04 | 2018-06-04 | 测序接头、测序接头组和超低频突变的检测方法 |
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Publication Number | Publication Date |
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CN108893466A true CN108893466A (zh) | 2018-11-27 |
CN108893466B CN108893466B (zh) | 2021-04-13 |
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CN109576347A (zh) * | 2018-12-06 | 2019-04-05 | 深圳海普洛斯医疗器械有限公司 | 含单分子标签的测序接头和测序文库的构建方法 |
CN109652337A (zh) * | 2019-01-23 | 2019-04-19 | 浙江大学 | 校正高通量测序原核与真核微生物基因序列读取数的方法及所用菌 |
CN109680054A (zh) * | 2019-01-15 | 2019-04-26 | 北京中源维康基因科技有限公司 | 一种低频dna突变的检测方法 |
CN109706219A (zh) * | 2018-12-20 | 2019-05-03 | 臻和(北京)科技有限公司 | 构建测序文库的方法、试剂盒、上机方法及测序数据的拆分方法 |
CN109777866A (zh) * | 2018-12-29 | 2019-05-21 | 杭州迪安医学检验中心有限公司 | 一种用于二代测序技术检测dna低频变异的分子标签及其应用 |
CN110331187A (zh) * | 2019-08-12 | 2019-10-15 | 天津华大医学检验所有限公司 | 组合标签、组合标签接头及其应用 |
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CN112176419A (zh) * | 2019-10-16 | 2021-01-05 | 中国医学科学院肿瘤医院 | 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法 |
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