CN104350952A - Cultivation method for increasing polysaccharide content of tremella - Google Patents
Cultivation method for increasing polysaccharide content of tremella Download PDFInfo
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- CN104350952A CN104350952A CN201410690514.4A CN201410690514A CN104350952A CN 104350952 A CN104350952 A CN 104350952A CN 201410690514 A CN201410690514 A CN 201410690514A CN 104350952 A CN104350952 A CN 104350952A
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 27
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 27
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 27
- 241001506047 Tremella Species 0.000 title claims abstract description 15
- 238000012364 cultivation method Methods 0.000 title claims abstract description 14
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 241000233866 Fungi Species 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 9
- 235000011089 carbon dioxide Nutrition 0.000 claims description 8
- 241000609240 Ambelania acida Species 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000010905 bagasse Substances 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052602 gypsum Inorganic materials 0.000 claims description 6
- 239000010440 gypsum Substances 0.000 claims description 6
- 241000411851 herbal medicine Species 0.000 claims description 6
- 230000000422 nocturnal effect Effects 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 239000010902 straw Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract 1
- 239000001569 carbon dioxide Substances 0.000 abstract 1
- 239000003337 fertilizer Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 238000009825 accumulation Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a cultivation method for increasing the polysaccharide content of tremella. The cultivation method comprises various measures of: selecting a stock culture medium, a cultispecies culture medium, a substrate and the color of a film, managing fertilizers and water, controlling temperature difference between day and night and carbon dioxide in a greenhouse and the like, and finally, the polysaccharide content of the tremella treated by the cultivation method is increased by 16%.
Description
Technical field
The present invention relates to a kind of cultivation method, be specifically related to a kind of cultivation method improving tremella polysaccharides content.
Background technology
In fungal studies, find that many polysaccharide materials not only have antitumor and higher immunization in recent years, and there is good pharmacology and clinical utility, and be developed to clinical medicine.Modern scientific research proves, and edible fungi polysaccharide all has humidification to immunity of organism and cellular immunity; There is excited function to reticuloendothelial system, have protective effect to animal's liver; There is antiviral, antineoplastic immunization of wide spectrum and the dissolution to cholesterol.At present, methods for the treatment of in the world for the viroses of plant not yet finds suitable medicament, therefore, finding immunopotentiating agent is one of direction of Developing new drug, and fungi polysaccharide is desirable Novel immune medicament, its feature is to the almost non-toxic property of host plant, shows curative effect by the defense mechanism of host own.
Along with the intensification of industrialization degree, while developing the economy, also the pollution of environment is brought, particularly heavy metal pollution is in aggravation, and edible fungus culturing raw material also have received pollution in various degree, if we still conventionally cultivate, the edible mushroom cultivated, also more or less may can contain pollutant, cause the nutritive value of edible mushroom to decline, reduce the health-care efficacy of edible mushroom.
Research at present for fungi polysaccharide has had a part of progress, mainly concentrates on the aspects such as the extracting method how extracting fungi polysaccharide and how to improve polyoses content, and also rarely known by the people for the research of the cultivation aspect how improving tremella polysaccharides.
Summary of the invention
For the present situation existed at present, the technical problem that the present invention mainly solves is to provide a kind of cultivation method that can improve tremella polysaccharides, by controlling the day and night temperature of booth, and the amount of carbonic acid gas improves the accumulation of polysaccharide, and improves the content of polysaccharide by suitable culture technique.For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
Improve a cultivation method for tremella polysaccharides content, it is characterized in that, adopt following methods to cultivate:
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 2 ~ 6 days in described medium, then under temperature is 40 ~ 50 DEG C of conditions, induce 6 ~ 12 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 3 ~ 5 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 120 ~ 200 parts, cow dung 90 ~ 160 parts, herbal medicine slag 20 ~ 40 parts, wheat skin 40 ~ 70 parts, sucrose 6 ~ 12 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 4 ~ 6 days, keep day and night temperature to be 20 ~ 30 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.12 ~ 0.14%;
(7) timely collecting: when auricle fully launches, about eight to ninety percent ripe time gather.
Be: a kind of cultivation method improving tremella polysaccharides content adopt following methods to cultivate in a preferred embodiment of the present invention:
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 4 days in described medium, then under temperature is 45 DEG C of conditions, induce 9 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 4 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 160 parts, cow dung 120 parts, herbal medicine slag 30 parts, wheat skin 55 parts, sucrose 8 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 5 days, keep day and night temperature to be 25 ~ 30 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.13%;
(7) timely collecting: when auricle fully launches, about eight to ninety percent ripe time gather.
The invention has the beneficial effects as follows:
Medium of the present invention is applicable to the polysaccharide accumulation of white fungus.Improved the accumulation of polysaccharide by the concentration controlling day and night temperature and carbonic acid gas, more easily realize in booth.
Cultivation method of the present invention can improve the polyoses content in white fungus effectively, and it is low to consume energy, and cost increases few, and method is simple, is easy to control.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
Embodiment 1
Improve a cultivation method for tremella polysaccharides content, it is characterized in that, adopt following methods to cultivate:
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 2 days in described medium, then under temperature is 50 DEG C of conditions, induce 6 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 3 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 120 parts, cow dung 90 parts, herbal medicine slag 20 parts, wheat skin 40 parts, sucrose 6 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 4 days, keep day and night temperature to be 20 ~ 25 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.12%;
(7) timely collecting: when auricle fully launches, about eight to ninety percent ripe time gather.
After measured, active polysaccharide on average increases by 12% to tremella polysaccharides of the present invention.
Embodiment 2
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 4 days in described medium, then under temperature is 45 DEG C of conditions, induce 9 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 4 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 160 parts, cow dung 120 parts, herbal medicine slag 30 parts, wheat skin 55 parts, sucrose 8 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 5 days, keep day and night temperature to be 25 ~ 30 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.13%;
After measured, active polysaccharide on average increases by 16% to tremella polysaccharides of the present invention.
Embodiment 3
Improve a cultivation method for tremella polysaccharides content, it is characterized in that, adopt following methods to cultivate:
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 6 days in described medium, then under temperature is 40 DEG C of conditions, induce 12 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 5 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 200 parts, cow dung 160 parts, herbal medicine slag 40 parts, wheat skin 70 parts, sucrose 12 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 6 days, keep day and night temperature to be 25 ~ 30 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.14%;
(7) timely collecting: when auricle fully launches, about eight to ninety percent ripe time gather.
After measured, active polysaccharide on average increases by 14% to tremella polysaccharides of the present invention.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (1)
1. improve a cultivation method for tremella polysaccharides content, it is characterized in that, adopt following methods to cultivate:
(1) following mother culture media is selected: potato 200 grams, glucose 20 grams, Cobastab
11 gram, MgSO
41.5 grams, KH
2pO
43 grams, 1000 milliliters, water, pH value nature;
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, corn flour 10%, wheat bran 12%, analysis for soybean powder 4%, sucrose 1%, gypsum 1%; Take out after white fungus bacterial strain is cultivated 2 ~ 6 days in described medium, then under temperature is 40 ~ 50 DEG C of conditions, induce 6 ~ 12 minutes;
(3) continue to cultivate: the white fungus medium through above-mentioned process is placed in gas bath shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivates 3 ~ 5 days;
(4) set up frame, cover red or purple film;
(5) select suitable nutrient matrix to cultivate, described nutrient matrix is formulated by the component of following weight portion, straw 120 ~ 200 parts, cow dung 90 ~ 160 parts, herbal medicine slag 20 ~ 40 parts, wheat skin 40 ~ 70 parts, sucrose 6 ~ 12 parts;
(6) temperature of shed is controlled, daytime is the plastic house of plantation white fungus, improve temperature of shed, raise film when nocturnal temperature is minimum and reduce mushroom bed tempertaure, repeatedly operate 4 ~ 6 days, keep day and night temperature to be 20 ~ 30 DEG C, keep canopy humidity 85-90%, the concentration controlling carbonic acid gas in canopy is 0.12 ~ 0.14%;
(7) timely collecting: when auricle fully launches, about eight to ninety percent ripe time gather.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410690514.4A CN104350952B (en) | 2014-11-25 | 2014-11-25 | A kind of cultural method improving tremella polysaccharide content |
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|---|---|---|---|
| CN201410690514.4A CN104350952B (en) | 2014-11-25 | 2014-11-25 | A kind of cultural method improving tremella polysaccharide content |
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| Publication Number | Publication Date |
|---|---|
| CN104350952A true CN104350952A (en) | 2015-02-18 |
| CN104350952B CN104350952B (en) | 2016-09-21 |
Family
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123268A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | High-yield tremella planting method |
| CN105315065A (en) * | 2015-05-29 | 2016-02-10 | 姚淑先 | Chinese herbal medicine tremella cultivation technology |
| CN106305142A (en) * | 2016-08-23 | 2017-01-11 | 李军 | Method for growing tremella by utilizing fungi grass |
| CN106905064A (en) * | 2017-04-18 | 2017-06-30 | 无限极（中国）有限公司 | A kind of cultivating white fungus culture medium and preparation method thereof, cultivating white fungus method |
| CN107162808A (en) * | 2017-07-14 | 2017-09-15 | 华南农业大学 | Improve the preparation method of tremella polysaccharides content culture medium for cultivating |
| CN107493965A (en) * | 2017-08-29 | 2017-12-22 | 贵州普定印象朵贝农业开发有限公司 | A kind of implantation methods for improving white fungus protein content |
| CN112243798A (en) * | 2020-10-22 | 2021-01-22 | 山东同康农业开发有限公司 | Energy-saving and efficient tremella strain breeding and planting method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315065A (en) * | 2015-05-29 | 2016-02-10 | 姚淑先 | Chinese herbal medicine tremella cultivation technology |
| CN105123268A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | High-yield tremella planting method |
| CN106305142A (en) * | 2016-08-23 | 2017-01-11 | 李军 | Method for growing tremella by utilizing fungi grass |
| CN106905064A (en) * | 2017-04-18 | 2017-06-30 | 无限极（中国）有限公司 | A kind of cultivating white fungus culture medium and preparation method thereof, cultivating white fungus method |
| CN107162808A (en) * | 2017-07-14 | 2017-09-15 | 华南农业大学 | Improve the preparation method of tremella polysaccharides content culture medium for cultivating |
| CN107162808B (en) * | 2017-07-14 | 2020-06-02 | 华南农业大学 | Preparation method of culture medium for improving content of tremella polysaccharide |
| CN107493965A (en) * | 2017-08-29 | 2017-12-22 | 贵州普定印象朵贝农业开发有限公司 | A kind of implantation methods for improving white fungus protein content |
| CN112243798A (en) * | 2020-10-22 | 2021-01-22 | 山东同康农业开发有限公司 | Energy-saving and efficient tremella strain breeding and planting method |
| CN112243798B (en) * | 2020-10-22 | 2022-02-22 | 山东同康农业开发有限公司 | Energy-saving and efficient tremella strain breeding and planting method |
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| Publication number | Publication date |
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| CN104350952B (en) | 2016-09-21 |
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