CN106497821A - A kind of food materials are used for the method for probiotic's culture and its application - Google Patents
A kind of food materials are used for the method for probiotic's culture and its application Download PDFInfo
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- CN106497821A CN106497821A CN201610834396.9A CN201610834396A CN106497821A CN 106497821 A CN106497821 A CN 106497821A CN 201610834396 A CN201610834396 A CN 201610834396A CN 106497821 A CN106497821 A CN 106497821A
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- 235000014962 Streptococcus cremoris Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
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- 235000021307 Triticum Nutrition 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 108010055615 Zein Proteins 0.000 description 1
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940107666 astragalus root Drugs 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 1
- 229940009289 bifidobacterium lactis Drugs 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 239000006161 blood agar Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 235000015190 carrot juice Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 150000001868 cobalt Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229960003638 dopamine Drugs 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 229940095100 fulvic acid Drugs 0.000 description 1
- 239000002509 fulvic acid Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 229940046240 glucomannan Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940001882 lactobacillus reuteri Drugs 0.000 description 1
- 229940115286 lentinan Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000019707 mung bean protein Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 125000003748 selenium group Chemical class *[Se]* 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 235000011497 sour milk drink Nutrition 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 235000011496 sports drink Nutrition 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
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- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of food materials are used for the method for probiotic's culture and its application.Fermentation Flour product is crushed by the method, being dipped into can be in film forming solution, dry, obtain stable edibility foamed material, the material is added to inoculation of medium probiotic's culture, under Optimal Medium and fermentation condition, probio gathers the surface of edibility foamed material or internal or surface and inside, obtains the probio of state of aggregation, realizes probio High Density Cultivation, after culture terminates, prepared high density probiotic products are dried.The activity of the probio that the present invention is prepared is high, and heat-resisting acidproof resistance is direct-edible, can be widely used for the exploitation of the products such as food, food additives, health products and medicine.
Description
Technical field
The present invention relates to a kind of bacterium preparation method and applications, are used for probiotic's culture more particularly, to a kind of food materials
Method and its application.
Background technology
Probio, mono- words of English probiotics are derived from by Greek " for live ", are translated into " for life ", are
Viable bacteria containing physiologically active, when being taken in after appropriate quantity through oral or other administering modes by body, can be colonized in
Host simultaneously improves host's microecological balance, so as to play beneficial effect.Recent studies indicate that, the bacterium in human body is up to
Bacterium in kind more than 1000, particularly intestines and stomach is enriched the most, accounts for whole body micro-ecological bacterial sum 80% or so, is human body cell
10 times, it is described as " second genome " of the mankind.These bacteriums contribute to human consumption's food, produce vitamin to prevent food
The disease induced by middle bacterium, while stimulating immune system.With going deep into for research, find enteric microorganism in many chronic diseases
In disease and symptom, such as inflammation, obesity, diabetes, also cancer etc. also play key effect.The quantity of microorganism and gene dosage
Surprising, the strong health for affecting the mankind.These are found to be probiotic products in food, health products, medicine, cultivation, environment, health
The application in the fields such as articles for use brings huge motive force.At present, probio is widely used in Yoghourt, sour milk beverage, prebiotic
In the products such as bacteria powder, animal feed, environmental improvement, relatively complete and ripe probio industry is defined.
The production of probio is mainly using solid fermentation and big tank liquid deep layer fermenting method.Solid fermentation method is probio
Being inoculated on solid medium carries out fermented and cultured, Chinese invention patent (publication number：104830711 A of CN) invent disk
Installation for fermenting solid fermentation bacillus amyloliquefaciens, fermentation time are usually 48h～72h.Chinese invention patent (publication number：CN
105132321A) using the culture medium by material compositions such as wheat bran and dregs of beans, inoculation VREF carries out solid state fermentation, dries and produces
Viable bacteria content in product is up to 3.8 × 1010CFU/g.Similar also has Chinese patent (publication number：CN 101555510B；CN
101555511B；CN 103421710B；CN 102907563B；CN 103289937B；CN 1212391C) using dregs of beans,
The agricultural wastes such as wheat bran, corncob, rice bran, peanut shell, starch, corn flour, turf or the solid that agricultural product are main component
Culture medium, microbe inoculation carry out high density fermentation, and the viable count in product is essentially 1011CFU/g.This kind of product is due to adopting
Make culture medium with a large amount of agricultural wastes or agricultural product, be mainly used in animal feed or carry out the production of the metabolites such as medicine, training
Support after terminating, great majority cannot be used directly for the production of the products such as human food, health products and medicine, need more follow-up locating
Science and engineering skill could prepare Related product.Additionally, although solid fermentation simple production process, small investment, easily receive miscellaneous bacteria simultaneously again
Pollution, unstable product quality, the longer shortcoming of fermentation time.Big tank liquid deep layer fermenting method is to adopt modern fermentation technique,
Probio is inoculated in bioreactor and is aerated culture, with being easily controlled thalline content, the stable spy of product quality
Point.Chinese invention patent (publication number：105018408 A of CN) add rutin raising rhamnose bacterium lactobacillus in the medium
Fermenting speed and the viable count of probio, the maximum viable count of the probiotics fermention liquid that probiotics fermention growth 24h is obtained are reachable
Arrive about 109CFU/mL.But such method is higher to technical merit requirement, with respect to solid fermentation investment height, subsequent concentration and separation
The high cost of target product.It is well known that fermentation Downstream processing has conclusive effect to the quality of product, thus downstream adds
Separation and purifying cost in work accounts for the major part in fermentation totle drilling cost.Therefore, simplify zymotechnique, stabilized product quality is
Important technological problems in probio exploitation and market.
Content of the invention
It is an object of the invention to overcoming the shortcoming of prior art, there is provided a kind of method that food materials are used for probiotic's culture
And its application.The inventive method is in the culture medium containing the somatomedin for promoting growth of probiotics breeding to add porous
Food materials, inoculation probio simultaneously ferment, and porous food materials overcome traditional agriculture discarded object or agricultural product are difficult to directly eat not
Foot part, and good nutrition and enrichment carrier is provided to growth breeding.After culture terminates, direct separation of solid and liquid is simultaneously done
Dry, high density, the high activity state of aggregation probio of edibility is prepared, food, food additives, health products are can be widely used for
Exploitation with products such as medicine.
The technical solution used in the present invention is as follows：
First, a kind of method that food materials are used for probiotic's culture：
Fermentation Flour product, is obtained foaminess food materials, is broken into particle.
The fermentation Flour product, be flour, ground rice, corn flour and various coarse cereal powders make obtain bread, cake, steamed bun
The foaminess food materials such as head, rice cake and steamed sponge cake.
The mean pore size of the fermentation Flour product is 2 nanometers -2 millimeters, and porosity is 10%-85%, and porosity refers to
Be percentage that hole in porous material accounts for whole material volume..
The fermentation Flour product crushed particles size is 0.1-3 centimetre, and sheet size is 0.1-10 centimetre.
The fermentation Flour product crushed particles, insert the mixed of film-formable protein solution or polysaccharide solution or albumen and polysaccharide
Close and soak in solution and dry, stable edibility foamed material in aqueous systems is obtained.
Described can film forming solution, be the mixed solution of film-formable protein solution or polysaccharide solution or albumen and polysaccharide.
Protide such as collagen, gelatin, lactalbumin, fibroin, spider silk fibroin, casein sodium, fiber egg
In vain, keratin, soybean protein, mung bean protein, wheat gluten, zein, glutelin etc. derive from animal, plant and microorganism
Albumen and its derivative；
Polysaccharide such as chitin, shitosan, cellulose, lignin, glucan, agarose, FOS, soy oligosaccharides
Sugar, konjaku glucomannan, oligoisomaltose, xylo-oligosaccharide, oligomeric maltose, starch, dextrin, inulin, alginic acid and its salt,
Dextran, hyaluronic acid, algal polysaccharides, algal polysaccharide, spirulina polysaccharide, lichenin, lentinan, GL-B, silver
Fungus polysaccharides, Arabic gum, pectin, guar gum, xanthans carrageenan etc. are from the more of plant, animal, marine alga and microorganism
Carbohydrates and their derivative.
Described edibility foamed material is one or more of the above material, but is not limited to above material.
Edibility foamed material is placed in the medium, and culture medium is 1 with the volume ratio of edibility foamed material：0.01–
1：200, inoculation probio is simultaneously cultivated, and dries after separation of solid and liquid, and high density, the high activity for preparing edibility is prebiotic
Bacterium.
The culture is Anaerobic culturel, and the probio being inoculated with is the microorganism of anaerobism or amphimicrobian.
Anaerobic culturel is culture 12-48h at 30-42 DEG C.
After culture terminates, the probio gathers the surface of edibility foamed material or internal or surface and interior
Portion, obtains the probio of state of aggregation.
Described culture medium is liquid, and described edibility foamed material is solid-state, cultivating system can be Liquid Culture, half
Any one in three kinds of training methods of solid culture and solid culture.
Containing the somatomedin for promoting growth of probiotics breeding in described culture medium.
Containing the somatomedin such as whey fermentation liquid, collagen zymotic fluid, the potato that promote growth of probiotics breeding
Zymotic fluid, mushroom zymotic fluid or extract, soya-bean milk, tomato juice, carrot juice, corn juice, Cordyceps extract, glossy ganoderma carry
Take thing, dendrobium candidum extract, angelica extract, ginkgo biloba p.e, malt extract, ginseng class extract, Astragalus Root P.E,
Fructus Monordicae extract, inulin, yeast dextran, xylo-oligosaccharide, vitamin, amino acid, dopamine, phytic acid, fulvic acid, humic
One or more of acid, magnesium salts, zinc salt, manganese salt, calcium salt, molysite, cobalt salt, selenium salt etc..
Probio used is Bifidobacterium, bifidobacterium adolescentis, animal bifidobacteria, bifidobacterium lactis, not tally double
Discrimination bacillus, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactobacillus, lactobacillus acidophilus, Lactobacillus casei, curling
Lactobacillus, lactobacillus delbruockii subspecies bulgaricus (lactobacillus bulgaricus), Lactobacillus delbrueckii subspecies, lactobacillus fermenti, grignard
Lactobacillus, Lactobacillus helveticus, Yue Shi lactobacillus, lactobacillus paracasei, Lactobacillus plantarum, lactobacillus reuteri, rhamnose breast bar
Bacterium, Lactobacillus salivarius, streptococcus, streptococcus thermophilus, lactococcus, Lactococcus lactis subsp. lactis, Lactococcus lactis butterfat
Subspecies, Lactococcus lactis biacetyl subspecies, leuconostoc mesenteroides subsp mesenteroides, Propionibacterium, saccharomyces, Dbaly yeast
Number, candida, Torulopsis, Bacteroides, Fusobacterium, wine Coccus, Peptostreptococcus, Penicillium, hammer
Category, fusobacterium, Aerococcus, Pediococcus, bacillus, pluton category, enterococcus spp, Micrococcus, Wei Si Salmonellas
One or more of category etc., but it is not limited to above bacterial classification.
Described culture medium is YPD (saccharomycete), Medium of Bifidobacterium (bifidobacterium adolescentis, animal bifidobacteria, breast
Bifidobacterium, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum), MRS culture mediums (acidophilus breast bar
Bacterium, Lactobacillus casei, lactobacillus delbruockii subspecies bulgaricus, Rui Shi lactobacillus, Yue Shi lactobacillus, Lactobacillus plantarum, Luo Yishi
The double second of lactobacillus, Lactobacillus rhamnosus, Lactobacillus salivarius, lactobacillus paracasei, Lactococcus lactis subsp. lactis, Lactococcus lactis
Acyl subspecies, lactobacillus gasseri, Pediococcus, enterococcus spp, Wei Si Bordetella, honeybee ball category, Lactobacillus crispatus), SIIM0012
Culture medium (lactobacillus fermenti, Lactobacillus rhamnosus), M17 culture mediums (streptococcus thermophilus), lactic acid bacteria culturing medium (Lactococcus lactis
Subsp. cremoris), Leuconostoc mesenteroides culture medium (leuconostoc mesenteroides subsp mesenteroides), propionic acid bacteria culture medium (Propionibacterium
Category), 5 ° of B é wort agars (Debaryomyces, candida, Torulopsis), blood agar culture-medium (Bacteroides,
Fusobacterium), tomato juice culture medium (wine Coccus), PDA potatos, glucose agar medium (Penicillium), SICC0070
Butyric acid bacterium culture medium (fusobacterium), TSA tryptose soya agars (Aerococcus), broth bouillon (bacillus), LB
Culture medium (Micrococcus) etc..
In the culture medium, the probiotics bacterial liquid measure of inoculation is 0.1%-50% (v/v).0.1%-50% (v/v) refers to
Be volume that probiotics bacterial liquid is added be add after whole culture volume 0.1%-50%.
The concentration of the bacterium solution in probio inoculation bacterium solution is 108-109CFU/mL.
The inventive method adds edibility foamed material in the basal medium of each bacterial classification, while it is prebiotic to add promotion
The somatomedin of bacteria growing breeding, in the medium under multi-component synergy, the growth and breeding speed of probio is significantly
Lifted, and the anaerobism due to probio or amphimicrobian characteristic, probio gather the surface of edibility foamed material or
Internal or surface and inside, dry after separation of solid and liquid, directly obtain high activity, the high-density aggregation state probio of edibility,
Product quality is stable, and later separation link is simple, and cost greatly declines.
2nd, application of the probio in the product such as food or food additives, health products and medicine is prepared.
Food includes the Chinese styles such as the western-style cakes such as biscuit, bread, cake, rice cake, steamed bun, steamed stuffed bun, steamed twisted roll, moon cake, Zongzi
The beverages such as the dairy produces such as cake, Yoghourt, dairy products, water, Juice, sports drink, chocolate, preserved fruit, flour, flour product
Deng；Health products and medicine include pulvis, aqua product etc..
The invention has the beneficial effects as follows：
Probio prepared in accordance with the present invention, simplifies the downstream processing of Fermentation Engineering, stable and reliable product quality,
Separate and purifying cost declines to a great extent, with direct edibility, while having high density and high activity, can directly process and be prepared into
Probiotics leaven or probiotics preparation, are with a wide range of applications in the products such as food, health products and medicine.
Specific embodiment
The present invention is described in further detail with reference to specific embodiment.
Below by embodiment, described in further detail to patent of the present invention, but the present invention is not limited to these.
Embodiments of the invention are as follows：
Embodiment 1：
The rusk that bread is broken into 0.8-10 centimetre of size, the pore size of bread particle is 20 nanometers -1 millimeter,
Porosity is 80%, inserts immersion half an hour, separation of solid and liquid post-drying in the acetum of 2.5w.% shitosans.
Rusk after adding shitosan to process in the medium, culture medium are 1 with the volume ratio of rusk：100, the training
In foster base each component percentage by weight be MRS 75%, L-cysteine hydrochloride 1%, vitamin C 1%, inulin 5%, mushroom
Extract 10%, soya-bean milk 8%.Bifidobacterium is inoculated in culture medium, and Bifidobacterium is from Chinese Research for Industrial Microbial Germ preservation pipe
Reason center is buied, and Bifidobacterium adopts concentration for 109The bacterium solution of CFU/mL, inoculation bacterium solution amount are 20%.Anaerobic culturel, 37 DEG C of trainings
After foster 16h, separation of solid and liquid is dried, obtains edibility Bifidobacterium piece, and in product, the total viable count of Bifidobacterium is up to 8 × 1010-3
×1012CFU/g, viable count are affected by factors such as drying means, and in cryodesiccated product, viable count is higher.
Embodiment 2：
The particle that cake is broken into 0.3-1 centimetre of size, the pore size of bread particle is 5 nanometers -2 millimeters, hole
Rate is 50%, inserts in 2.5w.% collagen solutions and soaks 1 hour, drying at room temperature after separation of solid and liquid.
Cake particle after adding collagen to process in the medium, culture medium are 1 with the volume ratio of cake particle：
10, in the culture medium each component percentage by weight be MRS 80%, sucrose 4%, magnesium salts 1%, corn juice 5%, Shitake Mushroom P.E
5%, whey fermentation liquid 5%.Lactobacillus delbrueckii lactobacillus is inoculated in culture medium, Lactobacillus delbrueckii breast
Bacillus is buied from Chinese industrial Microbiological Culture Collection administrative center, Lactobacillus delbrueckii lactobacillus adopt concentration for
109The bacterium solution of CFU/mL, inoculation bacterium solution amount are 30%.Anaerobic culturel, after 37 DEG C of culture 24h, separation of solid and liquid is dried, and obtaining can
Feeding habits Lactobacillus delbrueckii lactobacillus particle, in product, the total viable count of Lactobacillus delbrueckii lactobacillus bulgaricus is up to 2
×1011-3×1013CFU/g CFU/g, viable count are affected by factors such as drying means, viable count in cryodesiccated product
Higher.
Embodiment 3：
The sheet that steamed bun is broken into 1-8 centimetre of size, the pore size of steamed bun particle is 50 nanometers -1 millimeter, porosity
For 50%, insert in 3w.% gumwaters and soak 1 hour, drying at room temperature after separation of solid and liquid.
Steamed bun piece after adding Arabic gum to process in the medium, in the culture medium, each component percentage by weight is YPD
85%, malt extract 4%, vitamin 1%, oyster mushroom extract 5%, Ganodenna Lucidum P.E 2%, collagen zymotic fluid 3%.Ferment
Mother is inoculated in culture medium, and saccharomycete is buied from Chinese industrial Microbiological Culture Collection administrative center, saccharomycete adopt concentration for
108The bacterium solution of CFU/mL, inoculation bacterium solution amount are 50%.After 35 DEG C of culture 24h, separation of solid and liquid is dried, obtains edibility saccharomycete
Piece, in product, the total viable count of saccharomycete is up to 3 × 1011-2×1013CFU/g, viable count are affected by factors such as drying means,
In cryodesiccated product, viable count is higher.
Embodiment 4：
Rice cake particle is added in culture medium, and the pore size of rice cake particle is 500 nanometers -3 millimeters, and porosity is 30%,
Insert immersion half an hour, drying at room temperature after separation of solid and liquid in 2.5w.% corn protein solutions.
Rice cake particle after adding zein to process in the medium, culture medium are 1 with the volume ratio of rice cake particle：1,
In the culture medium, each component percentage by weight is M17 78%, and potato extract 10%, straw mushroom zymotic fluid 5%, Radix Angelicae Sinensis are extracted
Thing 5%, tomato juice 2%.Lactobacillus plantarum is inoculated in culture medium, and Lactobacillus plantarum is from Chinese Research for Industrial Microbial Germ preservation pipe
Reason center is buied, and Lactobacillus plantarum adopts concentration for 109The bacterium solution of CFU/mL, inoculation bacterium solution amount are 15%.37 DEG C of culture 36h,
Separation of solid and liquid, dries, obtains edible plants lactobacillus particle, and in product, total viable count is up to 6 × 1010-7×1012CFU/g,
Viable count is affected by factors such as drying means, and in cryodesiccated product, viable count is higher.
Embodiment 5：
Steamed sponge cake particle is added in culture medium, and the pore size of steamed sponge cake particle is 500 nanometers -3 millimeters, and porosity is 30%,
Immersion half an hour in 5w.% sodium alginate solns is inserted, CaCl is being inserted2Soak 5 minutes in solution, separation of solid and liquid post-drying.
Steamed sponge cake particle marine alga acid treatment after is added in the medium, and culture medium is 1 with the volume ratio of steamed sponge cake particle：0.1,
In the culture medium each component percentage by weight be MRS 78%, xylo-oligosaccharide 5%, marine algae extract 5%, straw mushroom zymotic fluid 5%,
Inulin 5%, phytic acid 2%.Bifidobacterium infantis, bacillus, Lactobacillus helveticus combined inoculation in culture medium, above-mentioned bacterial classification from
Chinese industrial Microbiological Culture Collection administrative center is buied, and adopts concentration for 109The inoculation bacterium solution of CFU/mL, is inoculated with bacterium solution amount
Respectively 15%, 15%, 15%.37 DEG C of culture 36h, separation of solid and liquid are dried, are obtained edibility and train particle altogether, always live in product
Bacterium number is up to 2 × 1012-1×1014CFU/g, viable count are affected by factors such as drying means, viable bacteria in cryodesiccated product
Number is higher.
As seen from the above-described embodiment, the probio that prepared by the inventive method, simplifies the downstream processing of Fermentation Engineering,
There is direct edibility, while having high density and high activity, significant technique effect be projected with which.
Claims (10)
1. a kind of method that food materials are used for probiotic's culture, it is characterised in that：Will fermentation Flour product crush, be dipped into can film forming molten
In liquid, dry, prepare stable edibility foamed material in aqueous systems；The material is added to containing somatomedin
Culture medium in, the volume ratio of culture medium and edibility foamed material is 1：0.01–1：200, inoculation probio is simultaneously cultivated, and cultivates
Separation of solid and liquid, then drying afterwards prepares direct-edible probiotic composition.
2. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：The culture is to detest
Oxygen culture, the probio being inoculated with are the microorganism of anaerobism or amphimicrobian.
3. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：After culture terminates,
The probio gathers the surface of edibility foamed material or internal or surface and inside, obtains the highly dense of edibility
Degree, high-activity probiotics product.
4. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：Described culture medium
For liquid or semisolid, described porous material is solid-state.
5. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：The culture medium
In, the probiotics bacterial liquid measure of inoculation is 0.1%-50%.
6. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：Described edibility
The mean pore size of foamed material is 2 nanometers -2 millimeters, and porosity is 10%-85%.
7. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：Described fermentation face
Product is one or more combination in bread, cake, steamed bun, rice cake or steamed sponge cake.
8. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：Described fermentation face
Product is broken into graininess or sheet.
9. the method that a kind of food materials according to claim 1 are used for probiotic's culture, it is characterised in that：Described can film forming
Solution is the mixed solution of protein solution or polysaccharide solution or albumen and polysaccharide.
10. the application of the probio for being prepared by the arbitrary methods described of claim 1-9, it is characterised in that：The probio
Application in the products such as food, food additives, health products and medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107897475A (en) * | 2017-11-22 | 2018-04-13 | 东莞亿智食品有限公司 | A kind of oligomeric candy of probiotics and preparation method thereof |
| CN108004182A (en) * | 2017-12-31 | 2018-05-08 | 武汉市天辰生物科技有限公司 | One plant of lactobacillus paracasei and its application in aquaculture |
| CN108834764A (en) * | 2018-06-19 | 2018-11-20 | 贵州省贵福菌业发展有限公司 | A kind of preparation method of oil tea mushroom activated liquid strain |
| CN112566499A (en) * | 2019-07-11 | 2021-03-26 | 鲁翰升 | Functional health food product prepared by fermenting whey-containing dough |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101691568A (en) * | 2009-10-13 | 2010-04-07 | 哈尔滨美华生物技术股份有限公司 | Method of producing edible immobilized active probiotics |
| CN102031248A (en) * | 2010-11-05 | 2011-04-27 | 钟春燕 | Microbial live bacterium agent, preparation method and application thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN103598415A (en) * | 2013-11-08 | 2014-02-26 | 阜新蒙古族自治县巨源生物科技有限公司 | Sunflower seed meal microbial protein feed and preparation method thereof |
| CN104894098A (en) * | 2015-05-09 | 2015-09-09 | 浙江省农业科学院 | Immobilization probiotics leavening agent and preparing method thereof |
-
2016
- 2016-09-20 CN CN201610834396.9A patent/CN106497821A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101691568A (en) * | 2009-10-13 | 2010-04-07 | 哈尔滨美华生物技术股份有限公司 | Method of producing edible immobilized active probiotics |
| CN102031248A (en) * | 2010-11-05 | 2011-04-27 | 钟春燕 | Microbial live bacterium agent, preparation method and application thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN103598415A (en) * | 2013-11-08 | 2014-02-26 | 阜新蒙古族自治县巨源生物科技有限公司 | Sunflower seed meal microbial protein feed and preparation method thereof |
| CN104894098A (en) * | 2015-05-09 | 2015-09-09 | 浙江省农业科学院 | Immobilization probiotics leavening agent and preparing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 胡珊等: "益生菌制剂加工技术的研究概况", 《中国乳品工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107897475A (en) * | 2017-11-22 | 2018-04-13 | 东莞亿智食品有限公司 | A kind of oligomeric candy of probiotics and preparation method thereof |
| CN108004182A (en) * | 2017-12-31 | 2018-05-08 | 武汉市天辰生物科技有限公司 | One plant of lactobacillus paracasei and its application in aquaculture |
| CN108834764A (en) * | 2018-06-19 | 2018-11-20 | 贵州省贵福菌业发展有限公司 | A kind of preparation method of oil tea mushroom activated liquid strain |
| CN112566499A (en) * | 2019-07-11 | 2021-03-26 | 鲁翰升 | Functional health food product prepared by fermenting whey-containing dough |
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Application publication date: 20170315 |