CN108821896A - A kind of efficient foliage bacterial fertilizer of grape and the preparation method and application thereof - Google Patents
A kind of efficient foliage bacterial fertilizer of grape and the preparation method and application thereof Download PDFInfo
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- CN108821896A CN108821896A CN201811091662.9A CN201811091662A CN108821896A CN 108821896 A CN108821896 A CN 108821896A CN 201811091662 A CN201811091662 A CN 201811091662A CN 108821896 A CN108821896 A CN 108821896A
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- microbial inoculum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses efficient foliage bacterial fertilizer of a kind of grape and preparation method thereof, 5-10 containing nanometer selenium parts, 5-15 parts of Bacillus cercus microbial inoculum, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 4-8 parts of chitosan, EDTA5-10 parts, 8-15 parts of amino acid, micro- 8-15 parts of fertilizer, 45-65 parts of water.Grape Se content can be effectively increased and improve the yield of grape and improve grape quality.Have many advantages, such as that significant effect, green non-pollution, utilization rate of fertilizer are high, improve yield and quality, is low in cost and easy to spread.
Description
Technical field
The present invention relates to vinegrowing technologies and fertilising field, and in particular to a kind of efficient foliage bacterial fertilizer of grape and its preparation
Method and application.
Background technique
Selenium is one of the essential trace elements of the human body, and selenium participates in forming glutathione peroxidase, has anticancer, prevents
Cancer, cardioprotection and anti-aging, it is anti-oxidant with eliminate free radical, a variety of special healthcare functions such as improve the immunity of the human body.People
Intake selenium approach guarantee mainly by the food containing selenium, Chinese Soclety of Nutrition's special recommendation China resident selenium intake be 50~
200 μ g/ days, and selenium deficiency Area Inhabitants current selenium intake in China's is only 40~50 μ g/ days, is good for so human body selenium-supply diseases prevention improves
Kang Shuiping, optimal path are scientific tonic selenium-rich agricultural and sideline products.
Grape is famous fruit, is eaten raw or raisins processed, and is made wine, and the wine foot after wine brewing can mention food and drink acid, root and rattan medicine
With energy preventing or arresting vomiting, miscarriage prevention.Grape is the industry melting ecological benefits, economic benefit and social benefit and being integrated, and Development in Guizhou grape produces
Industry has advantageous resources advantage, is the important breakthrough mouth transformed agricultural production.In recent years, grape industry in Guizhou obtains
To very fast development, the ground such as In Qiannan, Qiandongnan Prefecture of Guizhou Province, Zunyi and Tongren City are mainly distributed, such as In Qiannan Sui Autonomous County of Sandu
" township of Chinese crystal grape " title is authorized by grape branch of Chinese Association of Agricultural Science Societies." three all grape ", " Acer palmatum ' Atropurpureum' grape " and " red rock Portugal
The brands such as grape " be even more it is popular among consumers and enjoy great prestige both at home and abroad, Guizhou grape industry has gone on the development road of " Brand Agriculture "
Road, Guizhou grape -growing areas is more than 500,000 mu at present.
But with the development of industry, the outstanding problems such as administrative skill level is backward, yield and quality is low are presented, and then are established excellent
Matter, grape corresponding cultivation techniques efficiently, ecological, safe are extremely urgent.The specific function and selenium element of Se-rich farm products are people
The necessary mineral trace element of body develops selenium-rich grape and has important practical significance for the sustainable development of grape industry
And strategic importance.Common selenium-enriched fertilizer is mainly the mixing of selenium mineral powder and conventional chemical fertilizers currently on the market, due to natural
Selenium element is not easy to plant absorption conversion with the presence of inorganic compound state, so be easy to causeing the waste of resource and environment dirty
Dye.Present invention seek to address that prior art problem and a kind of grape selenium-rich liquid bacterial manure is provided, which is logical
The vital metabolic activity for crossing Bacillus cercus and germ oligotrophy unit cell, converts organic selenium, while EDTA for nanometer selenium
With the chelating of the nutriments such as organic selenium, chitosan, amino acid and micro- fertilizer, enable grape leave preferably absorption and accumulation selenium member
Element.
Summary of the invention
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets grape at significant effect
The grape selenium-rich liquid bacterial manure of growing nutrient demand.
Technical scheme is as follows：
A kind of grape selenium-rich liquid bacterial manure, it is made from the following raw materials in parts by weight：5-10 parts of nanometer selenium, wax-like gemma bar
5-15 parts of bacteria agent, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 4-8 parts of chitosan, EDTA5-10 parts, 8-15 parts of amino acid,
Micro- 8-15 parts of fertilizer, 45-65 parts of water.
Preferably, it is made from the following raw materials in parts by weight：8 parts of nanometer selenium, 10 parts of Bacillus cercus microbial inoculum, thermophilic malt
It is 10 parts of Stenotrophomonas microbial inoculum, 6 parts of chitosan, 8 parts of EDTA, 0 part of amino acid 1, fertile 12 parts, 36 parts of water micro-.
Wherein, the strain contained in said waxy gemma bacillus agent is Bacillus cercus LY05 (CCTCC NO:M
2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more)；The strain of the germ oligotrophy unit cell microbial inoculum is thermophilic malt oligotrophy list
Born of the same parents bacterium XQ08 (CCTCC NO:M 2015726).The microbial inoculum preparation includes the following steps：(1) bacterial strain inclined-plane is saved into test tube species
Son is seeded in minimal medium respectively, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with
The inoculum concentration of 10% percent by volume is seeded in the seed fermentation tank containing minimal medium, culture to logarithmic growth
Phase obtains seed liquor；(3) seed liquor of acquisition is inoculated in fermentation medium according to the inoculum concentration of 10% volume ratio,
30-35 DEG C, fermented and cultured 2-3 days under 150-200rpm, obtain fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL,
Fermentation liquid is directly diluted 5-20 times respectively and obtains Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent.
Further, the chitosan is water solubility, and deacetylation is greater than 95%, and molecular weight is 10~50KDa；The ammonia
Base acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, color ammonia
Acid, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, any five kinds in cysteine
Or more combination；The EDTA is disodium ethylene diamine tetraacetate；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-
2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer of 0.5-1；The water is
Sterile water.
The preparation method of grape selenium-rich liquid bacterial manure of the invention, comprises the technical steps that：
Step 1. takes obtained Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent by regulation proportion, standby
With；
Step 2. is taken nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in required proportion by regulation proportion
Sterile water in；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0~6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to grape selenium-rich liquid bacterial fertilizer products.
Compared with prior art, the present invention selenium-rich liquid bacterial manure of the invention is few by Bacillus cercus and thermophilic malt
The vital metabolic activity for supporting monad, converts organic selenium for nanometer selenium, at the same EDTA and organic selenium, chitosan, amino acid and
The chelating of the nutriments such as micro- fertilizer, enables grape leave preferably absorption and accumulation selenium element.With significant effect, green without dirt
Dye, the advantages that utilization rate of fertilizer is high, low in cost and easy to spread.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as any limitation of the invention.In this hair
Numerical value or number ratios involved in bright indicate mass figures or mass ratio unless otherwise instructed.
1. selenium-rich liquid bacterial manure composition and preparation：
Selenium-rich liquid bacterial manure formula composition and preparation, (1) composition：It is 8 parts of nanometer selenium, 10 parts of Bacillus cercus microbial inoculum, thermophilic
It is 10 parts of malt Stenotrophomonas microbial inoculum, 6 parts of chitosan, 8 parts of EDTA, 0 part of amino acid 1, fertile 12 parts, 36 parts of water micro-.(2) it prepares：
1. Bacillus cercus microbial inoculum and germ oligotrophy unit cell liquid bacterial agent are taken by regulation proportion, it is spare；2. being taken by regulation proportion
Nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer, which are sufficiently stirred, to be dissolved in the required sterile water matched；3. 1. step is obtained
Microbial inoculum be added in the solution that 2. obtains of step, under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, in sterile blender
It is sufficiently mixed uniformly, it is packed up to grape selenium-rich liquid bacterial manure.
2. field trial：
Experimental design：RANDOMIZED BLOCK DESIGN is taken in test, spraying using complete stool, if 200 times of liquid of selenium-rich liquid bacterial manure, spraying
Clear water is control (CK), and totally 2 processing, are repeated 4 times, 8 cells totally, around sets protection row.Administration time is budding period, prosperous growth
Phase applies respectively, and amount of application is respectively 30,45kg/ mus.Weather is based on fine during test, or it is cloudy, spray latter 3-4 days
Interior no rainfall.
3. analysis project and method：
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition,
Take back experimental determination fruit quality, single fruit weight.Nutrient quality measuring method：Dry matter is measured with National Standard Method method；Vitamin C contains
Amount is measured with 2,6 dichlorophenol indophenol method；Titratable acid (TA) content determination of acid-basetitration；Soluble solid (SSC) content
It is measured with PAL-1 type refractometer.Se content is measured using National Standard Method GB/5009.93-2010.
4. embodiment effect：
From table 1 to table 2 as can be seen that can effectively increase yield, extremely significant raising fruit using grape selenium-rich liquid bacterial manure
Dry matter, vitamin C and soluble solid (SSC) content reduce titratable acid (TA) content, promote the shape of vintage
At the improvement with quality；And can effectively grape fruit organic selenium, inorganic selenium and total Se content, improve the accounting of organic selenium, more have
It ingests conducive to people to selenium.
Table 1 applies influence of the selenium-rich liquid bacterial manure to vintage quality
Note：It indicates with different lowercases are marked after column data in P<0.05 lower difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 lower difference is same up to extremely significant horizontal following table
Influence of the 2 grape selenium-rich liquid bacterial manure of table to grape selenium content
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (5)
1. a kind of grape selenium-rich liquid bacterial manure, it is characterised in that：It is made from the following raw materials in parts by weight：5-10 parts of nanometer selenium,
5-15 parts of Bacillus cercus microbial inoculum, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 4-8 parts of chitosan, 5-10 parts of EDTA, ammonia
8-15 parts of base acid, micro- 8-15 parts of fertilizer, 45-65 parts of water.
2. grape selenium-rich liquid bacterial manure according to claim 1, it is characterised in that：It by following parts by weight raw material system
At：8 parts of nanometer selenium, 10 parts of Bacillus cercus microbial inoculum, 10 parts of germ oligotrophy unit cell microbial inoculum, 6 parts of chitosan, EDTA 8
It is part, 0 part of amino acid 1, fertile 12 parts, 36 parts of water micro-.
3. grape selenium-rich liquid bacterial manure according to claim 1 or 2, it is characterised in that：Said waxy gemma bacillus agent
In the strain that contains be Bacillus cercus LY05, one kind of Bacillus cercus LGY06 and Bacillus cercus WL08 or two
Kind or more combination；The strain of the germ oligotrophy unit cell microbial inoculum is germ oligotrophy unit cell XQ08；Two kinds of microbial inoculums
Preparation includes the following steps：(1) bacterial strain inclined-plane is saved into test tube seed, be seeded in minimal medium respectively, oscillation training
It supports to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with the inoculum concentration of 10% percent by volume be seeded to containing whether there is or not
In the seed fermentation tank of machine salt culture medium, culture to logarithmic growth phase obtains seed liquor；(3) by the seed liquor of acquisition according to
The inoculum concentration of 10% volume ratio is inoculated in fermentation medium, and fermented and cultured 2-3 days, is obtained at 30-35 DEG C, 150-200rpm
Fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL, fermentation liquid is directly diluted respectively 5-20 times obtain it is wax-like
Gemma bacillus agent, germ oligotrophy unit cell liquid bacterial agent.
4. grape selenium-rich liquid bacterial manure according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, is taken off
Acetyl degree is greater than 95%, and molecular weight is 10~50KDa；The amino acid is proline, glycine, glutamic acid, phenylalanine, bright
Propylhomoserin, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine,
Arginine, asparatate, any five kinds or more of combination in cysteine；The EDTA is ethylenediamine tetra-acetic acid two
Sodium；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer of 0.5-1+
Fertiliser containing magnesium+iron fertilizer+zinc fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer；The water is sterile water.
5. a kind of preparation method of grape selenium-rich liquid bacterial manure as claimed in claim 1 or 2, it is characterised in that：Including as follows
Processing step：
Step 1. takes obtained Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent, spare；
Step 2. takes nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportion；
Step 3. microbial inoculum that step 1 obtains is added in the solution that step 2 obtains, in 25-30 DEG C of temperature, pH 6.0~6.8
Under the conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to grape selenium-rich liquid bacterial fertilizer products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811091662.9A CN108821896A (en) | 2018-09-19 | 2018-09-19 | A kind of efficient foliage bacterial fertilizer of grape and the preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811091662.9A CN108821896A (en) | 2018-09-19 | 2018-09-19 | A kind of efficient foliage bacterial fertilizer of grape and the preparation method and application thereof |
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| CN108821896A true CN108821896A (en) | 2018-11-16 |
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| CN201811091662.9A Pending CN108821896A (en) | 2018-09-19 | 2018-09-19 | A kind of efficient foliage bacterial fertilizer of grape and the preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN111763112A (en) * | 2019-03-29 | 2020-10-13 | 张德珍 | Biological amino acid-integrated fruit, vegetable and grain leaf surface spraying agent containing nano-selenium |
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|---|---|---|---|---|
| CN102173927A (en) * | 2011-01-10 | 2011-09-07 | 金盈 | Nanometer selenium probiotics composite leaf surface nutrient solution and application thereof in production of selenium-enriched pears |
| CN102942392A (en) * | 2012-11-29 | 2013-02-27 | 晓健科技（大连）有限公司 | Selenium-enriched biofertilizer and preparation method thereof |
| CN105746292A (en) * | 2016-03-03 | 2016-07-13 | 安徽中谷生物科技有限公司 | Planting method for selenium-rich organic grapes |
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2018
- 2018-09-19 CN CN201811091662.9A patent/CN108821896A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173927A (en) * | 2011-01-10 | 2011-09-07 | 金盈 | Nanometer selenium probiotics composite leaf surface nutrient solution and application thereof in production of selenium-enriched pears |
| CN102942392A (en) * | 2012-11-29 | 2013-02-27 | 晓健科技（大连）有限公司 | Selenium-enriched biofertilizer and preparation method thereof |
| CN105746292A (en) * | 2016-03-03 | 2016-07-13 | 安徽中谷生物科技有限公司 | Planting method for selenium-rich organic grapes |
Non-Patent Citations (2)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN111763112A (en) * | 2019-03-29 | 2020-10-13 | 张德珍 | Biological amino acid-integrated fruit, vegetable and grain leaf surface spraying agent containing nano-selenium |
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Application publication date: 20181116 |