CN102942392A - Selenium-enriched biofertilizer and preparation method thereof - Google Patents

Abstract

The invention provides a selenium-enriched biofertilizer and a preparation method of the selenium-enriched biofertilizer by complexing active bacteria and nano-selenium elements. According to the selenium-enriched biofertilizer, by means of chemical and physical mutagenesis and the like, the active bacteria and the nano-selenium elements are complexed through a reasonable coordination mode. A new strain contains the nano-selenium elements which have low toxicity and good biological activity, thereby the strain can be fully absorbed by human body, and the requirement of the human body for the selenium can be met.

Description

A kind of Se-enriched bio bacterial manure and preparation method thereof

Technical field

The invention belongs to field of ecological agricultural, particularly a kind of Se-enriched bio bacterial manure and preparation method thereof.

Background technology

According to incompletely statistics, China has 2/3rds regional approximately 700,000,000 populations to be in to lack the selenium state.Therefore, utilize indirect gimmick Development and Production natural selenium-rich food, especially develop natural organic selenium vegetables etc., become the emphasis of present China selenium-enriched food.At present, the selenium-enriched food of producing on the market mainly be by Young Plant seedling stage to the ripening stage, the method of spraying Sodium Selenite at plant leaf surface or fruit improves the selenium content in the plant, but this method exists remaining Sodium Selenite to remain in the soil, will be with problems such as pollution fishes and shrimps in the inflow streams such as rainwater.This project overcomes the above problems, and a kind of rich selenium bacterial manure is provided, and is used for cultivating natural plant rich in selenium, again can be to environment.

Summary of the invention

The objective of the invention is to make viable bacteria and selenium element take rational coordination mode to carry out complexing by means such as chemistry and physical mutagenesis, produce novel selenium-enriched bio-bacterial manure.

Technical solution of the present invention is achieved in that a kind of Se-enriched bio bacterial manure, and the weight proportion of its three kinds of main active bacterial strains and nano-selenium element is as follows:

Root nodule bacterium 10-20%

Azotobacter 42-55%

Combination azotobacter 10-30%

Contain selenium microorganism 2-8%

The preparation ratio of substratum is:

Peptone 0.8-1.5%

Yeast extract 0.02-0.5%

Extractum carnis 4-15%

Salt 0.01-0.09%

Wheat bran 15-40%

Semen Maydis powder 10-30%

Sugar 1.0-4.5%

Husk 15-30%

Peat composed of rotten mosses powder 1.0-1.2%

Calcium carbonate 1.0-1.2%

Transferring pH value with alkaline ammoniacal liquor, lime or sodium hydroxide is 8 to make by sterilization.

It is wherein said that what contain that the selenium microorganism uses is nano-selenium element.

The production method that contains selenium bacterial manure, its concrete making step is:

1) seed selection contains selenium bacterium liquid

Wherein the depth of humus layer of pure natural vegetation is taken from the collection of root nodule bacterium, azotobacter, three kinds of active bacterial strains of combination azotobacter fully; With natural bacterial strain through purification, rejuvenation, UV treatment, plate isolation, shake-flask culture, vitality test, mutagenic treatment etc. is several again take turns circulation after, according to the hypertonicity of bacterial strain, complexing nanometer selenium and other trace elements satisfies nutritional requirement comprehensively;

The above-mentioned prefabricated concentrated solution that contains selenium bacterium liquid is quantitatively for subsequent use;

2) will contain the sterile aqueous media of peptone, wheat bran, Semen Maydis powder, sugar, wheat bran, peat composed of rotten mosses powder quantitatively for subsequent use;

3) in container with sterile aqueous media with contain selenium bacterium liquid mixing and stirring (substratum: containing selenium bacterium liquid is 1:0.3 ~ 0.5), a kind of accent pH value that re-uses in ammoniacal liquor, lime or the sodium hydroxide is 8;

4) with above-mentioned 3) in the substratum that stirs carry out wet sterilization, then use infrared ray radiation 4-5 minute after, can pack prolonged preservation.

Advantage of the present invention mainly contains:

1) contain nano-selenium element in the novel bacterial, it is low toxicity not only, also possesses preferably biological activity, and people's physical efficiency is fully absorbed, and satisfies health to the demand of selenium element;

2) spawn activity greatly improves, and the vigor of bacterial classification strengthens, and through the bacterial strain of mutagenesis, can make microorganism synthetic ammonia, organic acid pump the extracellular in good time, so that the fixed nitrogen of microorganism, phosphorus decomposing, potassium decomposing, ability improves more than 20%;

3) compared with general foliage fertilizer low price 20-30%, and quality improvement, disease and pest reduces, and is the desirable fertilizer of producing green food or Organic food.

Embodiment

Embodiment (one)

Root nodule bacterium 17%, azotobacter 50%, combination azotobacter 25%, the ratio that contains selenium microorganism 8% are made; The preparation ratio of substratum is: peptone 1.2%, yeast extract 0.3%, extractum carnis 10%, salt 0.05%, wheat bran 35%, Semen Maydis powder 20%, sugar 3.0%, husk 28%, peat composed of rotten mosses powder 1.0%, calcium carbonate 1.0%.After will containing selenium bacterium liquid and substratum and mixing, be 8 to make bacterial manure by sterilization with alkaline ammoniacal liquor, lime or sodium hydroxide accent pH value.

Use bacterial manure 20kg of the present invention, after adding that water is rare and analysing 300 times, in pumpkin vegetative period, spray on Folium Cucurbitae and the pumpkin, spray 2-3 time, every minor tick 4-8 days, can improve the pumpkin selenium content was 0.085mg/kg, realizes the effect of increasing production of every mu of 20kg.

Claims (3)

1. Se-enriched bio bacterial manure and preparation method thereof is characterized in that: comprise three kinds of main active bacterial strains, wherein the weight proportion of three kinds of main active bacterial strains and nano-selenium element is as follows:

Root nodule bacterium 10-20%

Azotobacter 42-55%

Combination azotobacter 10-30%

Contain selenium microorganism 2-8%

The preparation ratio of substratum is:

Peptone 0.8-1.5%

Yeast extract 0.02-0.5%

Extractum carnis 4-15%

Salt 0.01-0.09%

Wheat bran 15-40%

Semen Maydis powder 10-30%

Sugar 1.0-4.5%

Husk 15-30%

Peat composed of rotten mosses powder 1.0-1.2%

Calcium carbonate 1.0-1.2%

Transferring pH value with alkaline ammoniacal liquor, lime or sodium hydroxide is 8 to make by sterilization.

2. a kind of Se-enriched bio bacterial manure according to claim 1 and preparation method thereof is characterized in that: the described nanometer selenium that contains the use of selenium microorganism.

3. Se-enriched bio bacterial manure and preparation method thereof, it is characterized in that: its concrete making step is:

1) seed selection contains selenium bacterium liquid

Wherein the depth of humus layer of pure natural vegetation is taken from the collection of root nodule bacterium, azotobacter, three kinds of active bacterial strains of combination azotobacter fully; With natural bacterial strain through purification, rejuvenation, UV treatment, plate isolation, shake-flask culture, vitality test, mutagenic treatment etc. is several again take turns circulation after, according to the hypertonicity of bacterial strain, complexing nanometer selenium and other trace elements satisfies nutritional requirement comprehensively;

The above-mentioned prefabricated concentrated solution that contains selenium bacterium liquid is quantitatively for subsequent use;

2) will contain the sterile aqueous media of peptone, wheat bran, Semen Maydis powder, sugar, wheat bran, peat composed of rotten mosses powder quantitatively for subsequent use;

3) in container with sterile aqueous media with contain selenium bacterium liquid mixing and stirring (substratum: containing selenium bacterium liquid is 1:0.3 ~ 0.5), a kind of accent pH value that re-uses in ammoniacal liquor, lime or the sodium hydroxide is 8;

4) substratum that stirs in above-mentioned (3) is carried out wet sterilization, then use infrared ray radiation 4-5 minute after, can pack prolonged preservation.