CN108740354A - A kind of anti-Crowding Stress feed addictive of cray and preparation method - Google Patents
A kind of anti-Crowding Stress feed addictive of cray and preparation method Download PDFInfo
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- CN108740354A CN108740354A CN201810566381.8A CN201810566381A CN108740354A CN 108740354 A CN108740354 A CN 108740354A CN 201810566381 A CN201810566381 A CN 201810566381A CN 108740354 A CN108740354 A CN 108740354A
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- cray
- disodium
- feed addictive
- crowding
- crowding stress
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- Marine Sciences & Fisheries (AREA)
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- Fodder In General (AREA)
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Abstract
The invention discloses a kind of anti-Crowding Stress feed addictive of cray and preparation methods, belong to aquaculture cray feed additive field.The additive is made of glycine, γ-aminobutyric acid, compound nucleotide, zeolite powder；The preparation method of the additive comprises the steps of：A, five kinds of 5'-AMP, 5'-UMP disodium, 5'-CMP disodium, 5'-GMP2Na, 5'-inosinic acid disodium nucleotide are taken to be crushed to 60-80 mesh sieve, according to 1:1:1:1:1 ratio was in small powder stirrer for mixing 15-20 minutes；B, glycine, γ-aminobutyric acid, zeolite powder be crushed into 60-80 mesh sieve respectively, then proportionally weighs the compound nucleotide obtained in above-mentioned substance and step A, is put into powder stirrer and stirs 30-45 minutes, it is made to be sufficiently mixed uniformly.Caused by the additive effectively overcomes Crowding Stress the problem of slow-growing and hypoimmunity etc., the deformed limb rate and the death rate of cray are significantly reduced.
Description
Technical field
The invention belongs to cray feed additive fields, and in particular to a kind of anti-Crowding Stress feed addictive of cray
And preparation method thereof.
Background technology
With China's culture fishery scale, intensivization development, high-density breeding is a kind of acquisition water body maximization profit
Method has become a kind of one mode of aquaculture.But excessively high breeding density can be to cultivated animals to sky
Between, the intensified competition of resource, so that its is generated Crowding Stress, thus lead to stress reaction.When aquiculture animal is lasting
When stress situation, a large amount of consumption of energy are not only resulted in, and the immune defense function of body can be made to be suppressed, to draw
Send out the outburst of aquaculture creature disease.Therefore, how effectively to reduce high-density breeding aquiculture animal is generated it is crowded
Stress is to improve one of the major issue of China's culture fishery high-efficient development.
Cray, scientific name Procambius clarkii (Procambarus clarkii), after introducing China from the 1930s,
Become a kind of important Freshwater shrimps breed variety in national cultivation extensively at present.However, cray artificial breeding at present is single
It is the biggest obstacle for restricting its development to produce relatively low.Main cause has：First, cray fighting by nature, the spy with cannibalism
Point has phenomena such as small to gobble, shell or soft shell shrimp are eaten in non-shell.Second is that being easy to cause cray generation after cultivation density height
Crowding Stress causes slow-growing and premunition to decline and cause disease and largely break out, and especially cray is propagated artificially in recent years
Under the conditions of white spot syndrome virus frequently occur, cause huge loss to cray aquaculture.Therefore, pass through nutrition
The ability that method improves the anti-Crowding Stress of cray will provide strong technology to cray aquaculture industry rapid and healthy
Support.
Invention content
The anti-Crowding Stress of cray caused by China's cray aquaculture middle-high density cultivation proposed for background technology
Inferior capabilities and the problem of cause illness outbreak, the purpose of the present invention is to provide a kind of feeds of the anti-Crowding Stress of cray to add
Add agent.The anti-Crowding Stress ability of cray under the conditions of can effectively improving intensive culture by adding this product in feed,
The cray autotomy and disease incidence that high-density breeding is brought are reduced, the single rate of cray can be improved to greatest extent, greatly
Promote cray aquaculture industry healthy and sustainable development.The product dosage is small, easy to implement the method, easy to operate, and significant effect is
It is a kind of to regulate and control the novel fodder additive of the anti-Crowding Stress of cray in terms of the nervous system and immune system two.
Another object of the present invention is to be to provide a kind of preparation method of the anti-Crowding Stress feed addictive of cray,
This method is easy to operate, easy to operation, can be used for large-scale industrial production.
In order to achieve the above purpose, the invention is realized by the following technical scheme：
A kind of feed addictive of the anti-Crowding Stress of cray, is made of the mass fraction of following raw material：
A kind of feed addictive of the anti-Crowding Stress of cray, is made of (preferred scope) the mass fraction of following raw material：
A kind of feed addictive of the anti-Crowding Stress of cray, is made of (best range) the mass fraction of following raw material：
A kind of feed addictive of the anti-Crowding Stress of cray, is made of (optimum value) the mass fraction of following raw material：
The compound nucleotide ingredient is 5'-AMP disodium, 5'-UMP disodium, 5'-CMP disodium, 5 '-guanosines
Acid disodium, 5'-inosinic acid disodium, five kinds of ingredients are according to 1:1:1:1:1 ratio mixes；
A kind of heretofore described anti-Crowding Stress feed addictive of cray, preparation method comprise the steps of：
A, 5'-AMP disodium, 5'-UMP disodium, 5'-CMP disodium, 5'-GMP2Na, 5'-inosinic acid are taken
Five kinds of nucleotide of disodium were crushed to 60-80 mesh sieve, according to 1:1:1:1:1 ratio is in small powder stirrer for mixing 15-20
Minute, obtain compound nucleotide；
B, glycine, γ-aminobutyric acid, zeolite powder be crushed into 60-80 mesh sieve respectively, then proportionally weighed
The compound nucleotide that substance and step A are obtained is stated, is put into powder stirrer and stirs 30-45 minutes, it is made to be sufficiently mixed uniformly,
Up to a kind of feed addictive of the anti-Crowding Stress of cray of the present invention.
A kind of above-mentioned anti-Crowding Stress feed addictive of cray adds application in cray artifical compound feed, especially
It is that additive effect is more preferably in larva of crayfish artifical compound feed.Additive amount in cultured fishes feed is by weight percentage
Content is 0.5-1.0%, the visual cray aquaculture model of specific additive amount, growth phase, feeding volume and breeding density situation into
The appropriate adjustment of row.
Compared with existing additive, the present invention has the following advantages and effects：
(1) present invention adds in cray artifical compound feed, and small dragon under the conditions of high-density breeding not only can be improved
The anti-Crowding Stress ability of shrimp reduces cray autotomy rate and the death rate, but also the growth performance of cray can be improved and exempt from
Epidemic disease power.
(2) glycine and γ-aminobutyric acid are two kinds of inhibitory neurotransmitters, meanwhile, glycine is also neurotransmitter
Glycine and γ-aminobutyric acid are used cooperatively by adjusting control agent by a certain percentage in of the invention, inhibit small dragon from neuromodulation system
The behavior mutually fought between shrimp effectively prevents under the conditions of high-density breeding damage caused by the mutual autotomy of cray and dead
It dies.
(3) glycine and nucleotide are two kinds of non-essential nutrients, under normal condition cray body can synthesize this two
Kind substance meets itself needs, but under the conditions of artificial breeding, is generated crowded due to fast-growth and breeding density height
Stress causes its demand to increase.Exogenous glycine and nucleotide are added in feed, can reduce two kinds of substances of body pair
Synthesis especially can avoid Crowding Stress and cray caused to grow to effectively improve the normal physiological function of cray body
Slow and low premunition problem.
(4) present invention adds 0.5~1.0% in cray feed, can significantly reduce small dragon under the conditions of high-density breeding
Deformed limb rate 30~50%, the death rate 15~25% of shrimp；Meanwhile cray growth performance 15~20% can be improved, immunity increases
It is strong by 20~40%, there is good application value to cray aquaculture development high density healthy aquaculture.
Description of the drawings
Fig. 1 is effect diagram of this product to cray deformed limb rate under the conditions of Crowding Stress.
Fig. 2 is effect diagram of this product to the cray death rate under the conditions of Crowding Stress.
Fig. 3 is effect diagram of this product to cray growth performance under the conditions of Crowding Stress.
Fig. 4 is effect diagram of this product to lysozyme activity in cray serum under the conditions of Crowding Stress.
Fig. 5 is effect diagram of this product to Determination of cortisol in cray serum under the conditions of Crowding Stress.
Specific implementation mode
Further illustrate the present invention with reference to embodiments, but embodiment does not do any type of limit to the present invention
It is fixed.
Embodiment 1
A kind of anti-Crowding Stress feed addictive of cray, is made of the mass fraction of following raw material：
The compound nucleotide ingredient is 5'-AMP disodium, 5'-UMP disodium, 5'-CMP disodium, 5 '-guanosines
Acid disodium, 5'-inosinic acid disodium, five kinds of ingredients are according to 1:1:1:1:1 ratio mixes；
The anti-Crowding Stress feed addictive of a kind of cray of the present invention, preparation method comprise the steps of：
A, 5'-AMP, 5'-UMP disodium, 5'-CMP disodium, 5'-GMP2Na, 5'-inosinic acid disodium are taken
Five kinds of nucleotide were crushed to 70 mesh sieve, according to 1:1:1:1:1 ratio obtained compound in small powder stirrer for mixing 20 minutes
Nucleotide；
B, glycine, γ-aminobutyric acid, zeolite powder be crushed into 70 mesh sieve respectively, then proportionally weighs above-mentioned object
The compound nucleotide that matter and step A are obtained, is put into powder stirrer and stirs 45 minutes, it is made to be sufficiently mixed uniformly to get one
The feed addictive of the kind anti-Crowding Stress of cray.
Embodiment 2
A kind of feed addictive of the anti-Crowding Stress of cray, it is made of the mass fraction of following raw material：
The compound nucleotide ingredient is 5'-AMP disodium, 5'-UMP disodium, 5'-CMP disodium, 5 '-guanosines
Acid disodium, 5'-inosinic acid disodium, five kinds of ingredients are according to 1:1:1:1:1 ratio mixes；
Its preparation process is same as Example 1.
Embodiment 3
A kind of feed addictive of the anti-Crowding Stress of cray, it is made of the mass fraction of following raw material：
The compound nucleotide ingredient is 5'-AMP disodium, 5'-UMP disodium, 5'-CMP disodium, 5 '-guanosines
Acid disodium, 5'-inosinic acid disodium, five kinds of ingredients are according to 1:1:1:1:1 ratio mixes；
Its preparation process is same as Example 1.
Test example
Culture experiment and effect：Experiment cray is averaged weight as (2.64 ± 0.26) g, and test site is located at Hubei Province
The indoor temperature control cultivating system of animal science institute of Changjiang University of Jingzhou City aquaculture base, experiment shrimp use specification for
The glass water vat of 125cm × 80cm × 50cm, when 10 pvc pipes (being about 10cm) are put for cray shell in each water vat bottom
Hide.Test period is on May 8th, 2017 to totally 28 days June 3, every morning 6:30, evening 6:30 be satiated with food feeding.
Test fish is randomly divided into 3 groups, 1 water vat of control group puts 20 tail crays in a suitable place to breed, and control group 2 and test group water vat put 60 tails in a suitable place to breed respectively
Cray, corresponding breeding density is respectively 20,60,60 tails/m2.Control group 1,2：Fed control group feed；Test group：Feeding pair
According to the additive of+1% example 1 of group feed.
Experiment feed formula used and nutritional ingredient, are shown in Table 1.
1. test feed formula of table and nutritional ingredient
After culture experiment carries out 4 weeks, the deformed limb rate, the death rate, rate of body weight gain of 3 groups of crays are counted respectively, while detecting 3 groups
Lysozyme activity and Determination of cortisol in cray serum.As a result, it has been found that compared with control group 1, control group 2 increases in breeding density
After adding, deformed limb rate, the death rate of cray are sharply increased, and growth rate is remarkably decreased.Meanwhile corticosteroid hormone in serum
Content has higher rising, lysozyme activity then to significantly reduce.However, adding the additive of 1% example 1 in control group feed
Afterwards, for test group compared with control group 2, deformed limb rate, the death rate of cray reduce 50% and 23.46%, rate of body weight gain liter respectively
It is high by 16.15%.Lysozyme activity increases 37.75% in serum, and corticosteroid hormone content has dropped 43.54%.
Lysozyme is a kind of nonspecific immune factors in serum, for shellfish, resists external pathogen
It relies primarily on the nospecific immunity factor to play a role, especially lysozyme is one of the leading indicator of cray premunition.And
Corticosteroid hormone is that cray is generated by interrenal tissue after by environment-stress and is discharged into blood.Therefore, in serum
Corticosteroid hormone content is seen as a signal index of the cray by Crowding Stress.
Above-mentioned test result shows that high-density breeding can make cray generate Crowding Stress, leads to its deformed limb rate, death rate
The problems such as raising, growth performance and immunity reduce.And added in feed the additive in 1% example 1 can then alleviate it is highly dense
The Crowding Stress that degree cultivation generates cray, significantly reduces its deformed limb rate and the death rate, meanwhile, caused by overcoming Crowding Stress
The problem of slow-growing and immunity reduction etc..Therefore, the present invention has cray aquaculture development high-density breeding
Important application value.
Claims (7)
1. a kind of anti-Crowding Stress feed addictive of cray, it is characterised in that be made of the mass fraction of following raw material：
The compound nucleotide ingredient be 5'-AMP, 5'-UMP disodium, 5'-CMP disodium, 5'-GMP2Na,
5'-inosinic acid disodium, five kinds of ingredients are according to 1:1:1:1:1 ratio mixes.
2. the anti-Crowding Stress feed addictive of a kind of cray according to claim 1, it is characterised in that by following raw material
Mass fraction composition：
3. the anti-Crowding Stress feed addictive of a kind of cray according to claim 1, it is characterised in that：
4. the anti-Crowding Stress feed addictive of a kind of cray according to claim 1, it is characterised in that by following raw material
Mass fraction composition：
5. the anti-Crowding Stress feed addictive of a kind of cray according to claim 1, it is characterised in that by following raw material
Mass fraction composition：
6. the anti-Crowding Stress feed addictive of a kind of cray according to claim 1, it is characterised in that by following raw material
Mass fraction composition：
7. according to a kind of any anti-Crowding Stress feed addictives of cray of claim 1-6, it is characterised in that it is made
Preparation Method comprises the steps of：
A, 5'-AMP, 5'-UMP disodium, 5'-CMP disodium, 5'-GMP2Na, five kinds of 5'-inosinic acid disodium are taken
Nucleotide was crushed to 60-80 mesh sieve, according to 1:1:1:1:1 ratio was obtained in small powder stirrer for mixing 15-20 minutes
Compound nucleotide；
B, glycine, γ-aminobutyric acid, zeolite powder be crushed into 60-80 mesh sieve respectively, then proportionally weighs above-mentioned object
The compound nucleotide that matter and step A are obtained, is put into powder stirrer and stirs 30-45 minutes, it is made to be sufficiently mixed uniformly, i.e.,
It can.
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| CN201810566381.8A CN108740354B (en) | 2018-06-05 | 2018-06-05 | Crayfish crowding stress resistant feed additive and preparation method thereof |
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| CN201810566381.8A CN108740354B (en) | 2018-06-05 | 2018-06-05 | Crayfish crowding stress resistant feed additive and preparation method thereof |
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