CN108719279A - A method of improving early pears stem apex Encapsulation dehydration cryopreservation effect - Google Patents
A method of improving early pears stem apex Encapsulation dehydration cryopreservation effect Download PDFInfo
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- CN108719279A CN108719279A CN201810545505.4A CN201810545505A CN108719279A CN 108719279 A CN108719279 A CN 108719279A CN 201810545505 A CN201810545505 A CN 201810545505A CN 108719279 A CN108719279 A CN 108719279A
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- stem apex
- sucrose
- dehydration
- graphene quantum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The method for improving early pears stem apex Encapsulation dehydration cryopreservation effect：Early pears stem apex is handled successively with four kinds of culture mediums containing carbon nanotube and graphene quantum dot, is remarkably improved survival rate after the jelly of stem apex.Compared with the prior art, early pears stem apex Encapsulation dehydration cryopreservation effect is good, survival rate is high, stem apex easily regenerates by the present invention.
Description
Technical field：
The present invention relates to a kind of methods improving plant stem apex Encapsulation dehydration cryopreservation effect, are exactly one
The method that kind improves early pears stem apex Encapsulation dehydration cryopreservation effect.
Background technology：
The plantation of China pear tree is with a long history, and it is with stronger accommodative ability of environment, at present the Changjiang river north and south it is equal there are many
Adapt to the special quality product kind in place of local environment.Precocious Pear Trees with Various Cultivars is commonly called as south early-maturing pear, and the operatic circle is substantially in June to August
Between it is ripe, the maturity period of more northern pears is significantly done sth. in advance, and the supply for having filled up summer fresh fruit is insufficient, deep joyous by the numerous common people
It meets and likes.The Woody plants reception room or parlour town towns He Tiandun pleasant Climate, the rainfall is plentiful, have advantageous condition, two
Jargonel excellent quality that small towns produces, special taste, tasty and refreshing succulence, nutrition are comprehensive, are formally awarded by Minister Agriculture of China within 2012
Give " national agricultural product geographical sign " title.Shangrao morning pears are not only made for fresh fruit and eat, and can also be used as bringing down a fever in medicine
Agent has promoting production of body fluid and nourishing the lung, clearing heat and eliminating phlegm, antipyretic relieves summer heat and other effects.Morning pears main planting site in Shangrao is Shangrao County reception room or parlour town and field
Dun Zhen.By cultivation in more than 300 years, " serissa serissoide ", " Calusena lansium disappears " two excellent local varieties of spy were cultivated in two small towns.
Morning pears special excellent local varieties in the two Shangrao are at present substantially with existing for Local variety, these Local varieties and the reception room or parlour town towns He Tiandun
Environment and pest and disease damage coevolution during, form the special and excellent character for adapting to local environment, and the operatic circle is sought
Health-care components very abundant is supported, is the rare natural gene library of Jiangxi jargonel.Cryopreservation is current plant germplasm resource
Effectively, safe and stable, economy, the method for long-term preservation.Encapsulation dehydration be by sample with it is calcium alginate embedded and dehydration after,
The method preserved in input liquid nitrogen.This method is easily mastered, and can mitigate dehydration, simplifies dehydration procedure, reduces environmental factor
Influence, and once can handle more material, there is larger development prospect in the preservation of plant germplasm resource.But it wraps
Bury evaporation cryopreservation there is also survival rates the problems such as low, preservation effect is bad.For these problems, the present invention develops
A method of early pears stem apex Encapsulation dehydration cryopreservation effect is improved, can be the long-term preservation of Shangrao morning pears germ plasm resource
Technical foundation is provided.
Invention content：
The object of the present invention is to provide a kind of survival rates, and the early pears stem apex Encapsulation dehydration of the easily regenerated raising of high, stem apex is ultralow
The method of warm preservation effect.Realizing the technical solution of the object of the invention is, a kind of early pears stem apex Encapsulation dehydration ultralow temperature of raising
The method of preservation effect, it is characterised in that have following steps：(1) it in superclean bench, cuts early pears stem apex and is inoculated with
In fluid nutrient medium one：+ 3% (w/v) sodium alginate of MS+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dots+
0.3M sucrose；(2) it draws stem apex and is transferred to fluid nutrient medium two：0.4M sucrose+0.3-0.5g/L carbon nanotubes+0.3-0.5g/L
Graphene quantum dot+0.1M CaCl2, carry out conventional embedding；(3) embedding pearl is transferred to fluid nutrient medium three：MS+0.3-
0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+0.5M sucrose carries out conventional preculture；(4) through air or silica gel
After conventional dehydration, liquid nitrogen routine preservation and routine are thawed, embedding pearl is transferred to solid regenerated culture medium four：MS+1-2mg/L thiophenes
Grand+0.1-0.3mg/L NAA+0.3-0.5g/L carbon nanotubes+0.3-0.5g/L graphene quantum dots+30g/L the sucrose of benzene+
Material is put into culturing room after inoculation and carries out normal condition culture, is remarkably improved survival rate after the jelly of stem apex by 7.5g/L agar.
Specific implementation mode：
In conjunction with following embodiments, the invention will be further described：
(1) it handles for the first time
In superclean bench, cuts early pears stem apex and be inoculated in fluid nutrient medium one：MS+0.3-0.5g/L carbon is received
Mitron+0.3-0.5g/L graphene quantum dots+3% (w/v) sodium alginate+0.3M sucrose；
(2) it handles for second
It draws stem apex and is transferred to fluid nutrient medium two：0.4M sucrose+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphite
Alkene quantum dot+0.1M CaCl2, carry out conventional embedding；
(3) third time is handled
Embedding pearl is transferred to fluid nutrient medium three：MS+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantums
+ 0.5M sucrose is selected, conventional preculture is carried out；
(4) the 4th processing
Through air or silica gel routinely dehydration, liquid nitrogen routine preservation and it is conventional thaw after, will embedding pearl be transferred to it is solid regenerated
Culture medium four：MS+1-2mg/L Thidiazuron+0.1-0.3mg/L NAA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphenes
Material is put into culturing room after inoculation and carries out normal condition culture, is remarkably improved by quantum dot+30g/L sucrose+7.5g/L agar
Survival rate after the jelly of stem apex.
Claims (1)
1. the method for improving early pears stem apex Encapsulation dehydration cryopreservation effect, it is characterised in that there is following steps：(1) super
In net workbench, cuts early pears stem apex and be inoculated in fluid nutrient medium one：MS+0.3-0.5g/L carbon nanotubes+0.3-
0.5g/L graphene quantum dots+3% (w/v) sodium alginate+0.3M sucrose；(2) it draws stem apex and is transferred to fluid nutrient medium two：
0.4M sucrose+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+0.1M CaCl2, carry out conventional embedding；
(3) embedding pearl is transferred to fluid nutrient medium three：MS+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dots+
0.5M sucrose carries out conventional preculture；(4) it after through air or silica gel, routinely dehydration, liquid nitrogen routine preservation and routine are thawed, will wrap
It buries pearl and is transferred to solid regenerated culture medium four：MS+1-2mg/L Thidiazuron+0.1-0.3mg/L NAA+0.3-0.5g/L carbon nanometers
Material is put into culturing room after inoculation and carries out routine by pipe+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L agar
CMC model is remarkably improved survival rate after the jelly of stem apex.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810545505.4A CN108719279B (en) | 2018-05-21 | 2018-05-21 | Method for improving ultralow-temperature preservation effect of early pear stem tip embedding dehydration method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810545505.4A CN108719279B (en) | 2018-05-21 | 2018-05-21 | Method for improving ultralow-temperature preservation effect of early pear stem tip embedding dehydration method |
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| Publication Number | Publication Date |
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| CN108719279A true CN108719279A (en) | 2018-11-02 |
| CN108719279B CN108719279B (en) | 2021-06-25 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452156A (en) * | 2018-11-28 | 2019-03-12 | 上饶师范学院 | A method of improving early pears stem apex detoxification efficiency |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| AU625795B2 (en) * | 1989-05-23 | 1992-07-16 | Societe Des Produits Nestle S.A. | A storage process for vegetable embryos |
| CN104170818A (en) * | 2014-09-15 | 2014-12-03 | 上海交通大学 | Method for optimizing vitrified cryopreservation effect of agapanthus embryonic calluses |
| CN104255707A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for improving preservation effect of cymbidium type protocorm |
-
2018
- 2018-05-21 CN CN201810545505.4A patent/CN108719279B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU625795B2 (en) * | 1989-05-23 | 1992-07-16 | Societe Des Produits Nestle S.A. | A storage process for vegetable embryos |
| CN104170818A (en) * | 2014-09-15 | 2014-12-03 | 上海交通大学 | Method for optimizing vitrified cryopreservation effect of agapanthus embryonic calluses |
| CN104255707A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for improving preservation effect of cymbidium type protocorm |
Non-Patent Citations (4)
| Title |
|---|
| TAKAO NIINO等: "Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry", 《PLANT SCIENCE》 * |
| 赵艳华等: "包埋干燥超低温保存苹果离体茎尖", 《园艺学报》 * |
| 赵艳华等: "梨离体茎尖超低温保存方法的比较研究", 《河北农业科学》 * |
| 郭文冰等: "《松树体细胞胚胎发生与超低温冻存技术》", 30 April 2018, 广州：广东科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452156A (en) * | 2018-11-28 | 2019-03-12 | 上饶师范学院 | A method of improving early pears stem apex detoxification efficiency |
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| CN108719279B (en) | 2021-06-25 |
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