CN102630708B - Stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and application method of stress-resistance and health-care mycorhiza microbial inoculum - Google Patents
Stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and application method of stress-resistance and health-care mycorhiza microbial inoculum Download PDFInfo
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- CN102630708B CN102630708B CN 201210086884 CN201210086884A CN102630708B CN 102630708 B CN102630708 B CN 102630708B CN 201210086884 CN201210086884 CN 201210086884 CN 201210086884 A CN201210086884 A CN 201210086884A CN 102630708 B CN102630708 B CN 102630708B
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Abstract
The invention discloses a stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and an application method of the stress-resistance and health-care mycorhiza microbial inoculum. The stress-resistance and health-care mycorhiza microbial inoculum is prepared from a mycorhiza microbial inoculum and naphthylacetic acid by compounding; the mycorhiza microbial inoculum is formed by fermenting Lactarius subdulcis or Lactarius akahatsu or Lactarius hatsudake through a solid fermentation culture medium. The stress-resistance and health-care mycorhiza microbial inoculum is used for carrying out slurry dipping treatment on seedling root systems or is buried into Cunninghamia lanceolata forest soil in a forestation process, so that not only can root-taking and growth of the Cunninghamia lanceolata be increased, but also Cunninghamia lanceolata vigor is enhanced and the stress resistance is improved; and therefore Cunninghamia lanceolata yellows, seedling damping-off and Pestalotiopsis apiculatus are prevented from happening.
Description
Technical field:
The present invention relates to a kind of degeneration-resistant health care bush mycorrhiza agent of Chinese fir forest plantation and application method thereof that is composited by plant growth regulator and Chinese fir mycorhiza bacterium, have and promote the Chang ﹑ of Chinese fir Sheng Gen ﹑ Sheng to increase productivity and health-care effect such as resistance.
Background technology:
Along with Chinese economic development, the raising of living standards of the people, forestry industry is also changed from small to big and is changed from weak to strong, and has stepped into the fast traffic lane of high speed development.Particularly China aspect such as Zhuan Huang ﹑ furniture and paper for daily use in Jian Zhu Ye ﹑ Shi increases the demand of timber in recent years, make merchantable log year consume the forest resources 1.5 billion cubic meters, and the timber forest year that China can fell growth less than 1 billion cubic meter, existing timber can not satisfy the demands far away.In the face of China's forestry products vast market prospect and forest resources stock is few and low-quality present situation, need afforestation on the one hand, guarantee as much as possible to take root faster and more root amount, strengthen the resistance of vegetation, improve the afforestation survival rate; Can pass through the upgrowth situation of the existing forest of improvement on the other hand, accelerate arboreal growth, improve trees moisture and nutrient metabolism, strengthen resistance, with raising forest productivity, thus the demand pressure in alleviation timber forest market.
Chinese fir is the Pinaceae aiphyllium, and its grain of wood is straight, the corrosion resistant insect protected, has higher economic value, is one of China's most important special product commerical tree species in south.Chinese fir is subjected to influence of various factors such as residing Huan border Tiao spare ﹑ natural calamity and self resistance, and its upgrowth situation is far different, and wherein self resistance of plant is influence the Chinese fir the most direct factor of growing.Yet, to studies show that of occurrence regularities such as the Chinese fir seedling Cu ﹑ of Daoing Bing yellows and China fir seedling pestalotiopsis funerea, the Fa Sheng ﹑ of Chinese fir disease development has close ties with the growth physiological status of Chinese fir again: the germ great majority infect the weak plant of growth, present the susceptible disease of Chinese fir of physiology yellow.Therefore, all undesirable elements that causes the Chinese fir growth all can promote the generation of disease.As the area of China's southern hills red earth area and soil depletion, because of the nitrogenous ﹑ phosphorus of institute and organic few in the soil, add stickingly heavily harden, permeable bad, drought and waterlogging, influence such as subterranean water level is too high and tree vigo(u)r is weak, can both cause the generation of disease.
In the forest Pest management, all be the method that adopts chemical control for a long time, its easy quick-acting and economical and practical advantage is self-evident.Yet along with the lifting of science and technology development and people's environmental consciousness, the ecological awareness and health perception, problem such as the toxicity that chemical pesticide in use produces, residual, ecology and environmental pollution has caused extensive concern.For the use that reduces chemical pesticide and the deficiency that solves chemical pesticide, one side is at host's dominance characteristics of Chinese fir disease, handle the afforestation seedling or grow into forest with plant growth regulator, activate the activity of root original hase, induce promotion to take root fast and the plant growth, improve host's itself growth potential and growing power level, strengthen the quick-acting disease-resistant ability of Chinese fir.Adopt Chinese fir ectotrophic mycorrhiza technology on the other hand, utilize the activity of mycorhiza bacterium to accelerate the soil nutrient circulation, improve soil structure, promote that forest takes root and grows.Simultaneously, mycorhiza can also help the host to adapt to saline and alkaline Huan Jing ﹑ to reduce body physiological Du ﹑ and increase the absorption of moisture and utilize ability and with pathogenic bacteria dissolving or the effect of killing, hold the effect resistance thereby improve Chinese fir, fundamentally prevent the generation of Chinese fir yellows and the seedling Cu ﹑ of Daoing Bing pestalotiopsis funerea.
Chinese fir is the ectotrophic mycorrhiza plant, and the ectotrophic mycorrhiza plant is being subjected to extensive concern aspect the degeneration-resistant border of plant nutrition Dai Xie ﹑ and the disease and insect resistance.At present, the ectotrophic mycorrhiza technology has obtained first-stage success other many seeds in the application aspect Yin Zhong ﹑ mycorrhizal seedling raising afforestation, the adverse circumstance Zao Lin ﹑ control of plant disease.
Summary of the invention:
The purpose of this invention is to provide degeneration-resistant health care bush mycorrhiza agent and the application method thereof of a kind of Chinese fir, this degeneration-resistant health care bush mycorrhiza agent has strengthened the ability of Chinese fir resisting etiolation disease and China fir seedling damping off and pestalotiopsis funerea, has improved survival rate and the growth rate of China fir seedling.
Technical scheme of the present invention is:
The degeneration-resistant health care bush mycorrhiza agent of a kind of Chinese fir, by bush mycorrhiza agent: naphthalene acid solution is formulated according to weight ratio=800-1000:1; Described bush mycorrhiza agent is that pinnacle breast mushroom (Lactarius subdulcis) and orange red newborn mushroom (Lactarius akahatsu) and the newborn mushroom of red juice (Lactarius hatsudake) are adopted ferment in the culture that forms one or more of solid fermentation medium respectively; Described naphthalene acid solution concentration is 900ppm;
Before solid fermentation is cultivated, earlier pinnacle breast mushroom (Lactarius subdulcis) or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) are obtained bacterium liquid through inclined-plane solid culture medium and liquid shaking table medium culture, be inoculated into then in the solid fermentation medium and cultivate; Described solid fermentation culture medium prescription is: the peat composed of rotten mosses: wheat bran: corn flour: sucrose is pressed mass ratio=20-30:10-15:5-10:1; Bacterium liquid inoculum concentration is the 4-6% of solid fermentation culture volume, and fermentation temperature is 25 ℃-28 ℃, fermentation time 7-12 days;
Described liquid shaking table condition of culture: liquid nutrient medium adopts the PD medium that does not add agar; Aseptic card punch with diameter 6mm, cut pinnacle breast mushroom (Lactarius subdulcis) or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) the bacterium sheet cultivated on the inclined-plane solid plate, be inoculated in the 500ml triangular flask that fills the 250ml liquid nutrient medium every bottle graft kind 3-5 sheet; Put 25 ℃-28 ℃, rotating speed 200-220rmin
– 17-10d is cultivated in water bath with thermostatic control on the shaking table, obtains bacterium liquid;
Described pinnacle breast mushroom (Lactarius subdulcis) or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) are all picked up from the Chinese fir woods.
Described degeneration-resistant health care bush mycorrhiza agent is when preparation, and need mix with naphthalene acid solution after bush mycorrhiza agent is crushed to the 2-3mm size again.
Described inclined-plane solid culture condition: adopt the PDA medium: 20wt% potato juice 1000mL, glucose 18g, beef extract 15g, (NH
4)
2HPO
41g, magnesium sulfate 1.2g, agar 20g, vitaminB10 .01g; The cultivation temperature scope is 25 ℃-28 ℃; The pH scope is 5-6; Make medium and be used for primary inclined plane cultivation 7-10 days.
The application method of above-mentioned degeneration-resistant health care bush mycorrhiza agent: be applied in the soil of Chinese fir tree root or plantation Chinese fir, promote the growth of Chinese fir, improve Chinese fir resistance, thereby strengthen the ability of Chinese fir resisting etiolation disease and China fir seedling damping off and pestalotiopsis funerea, improve survival rate and the growth rate of Chinese fir.Specifically be when the Chinese fir seedling is afforested, after the ratio of 1:1.5 is fully mixed by weight with timbered soil with described degeneration-resistant health care bush mycorrhiza agent, again according to 1 kilogram of degeneration-resistant health care bush mycorrhiza agent: the ratio of 40 kg water adds water makes mud, and the Chinese fir seedlings root is dipped in slurry, then plantation.Or when Chinese fir manually grows into forest, add the polyacrylamide type water-loss reducer of 25g in the described degeneration-resistant health care bush mycorrhiza agent of every 225g, after fully mixing, digging pit to imbed in the soil of 1 meter of distance tree body gets final product.Preferably four different directions are digged pit in the soil of 1 meter of distance tree body, and the degree of depth in hole is 25cm, and the amount that bury in each hole is 250 grams.
Useful result of the present invention is: not only promoted taking root and growing of Chinese fir, also improved Chinese fir resistance, particularly strengthened the ability of Chinese fir resisting etiolation disease and China fir seedling damping off and pestalotiopsis funerea, survival rate and the growth rate of Chinese fir have been improved, improved soil condition, meet the requirement of sustainable development, be fit to the industrialization production of Chinese fir.
Embodiment:
Be intended to further specify the present invention below in conjunction with embodiment, and unrestricted the present invention.
Embodiment 1:
Employing connects the bacterium test, the pinnacle breast mushroom Lactarius subdulcis that test screening Chinese fir woods is plucked, orange red newborn mushroom Lactarius akahatsu, red juice breast mushroom Lactarius hatsudake, fragrant newborn mushroom Lactarius camphorates, sweet taste breast mushroom Lactarius glyciosmus, the newborn mushroom Lactarius subzonarius of fragrant inferior ring, the peaceful newborn mushroom Lactarius porninsis of ripple, the fine and soft newborn mushroom Lactarius gerardii of rare pleat, thick matter breast mushroom Lactarius deterrimus, fine hair breast mushroom Lactarius echinatus, rare pleat breast mushroom Lactarius hygrophoroides, suede Virgin's milk mushroom Lactarius vellereus, 13 kinds of newborn mushroom microbial inoculum inoculations such as succulence breast mushroom Lactarius volemus are to the influence of 2 years living China fir plant percents.Inoculate after 3 months, the survival rate that discovery connects orange red newborn mushroom (Lactarius akahatsu), red juice breast mushroom (Lactarius hatsudake), pinnacle breast mushroom (Lactarius subdulcis) China fir nursery stock is the highest, the survival rate of nursery stock is respectively 72.23%, 67.69% and 63.81%, improve 21.16%, 15.58% and 14.34% than contrast, the survival rate of nursery stock of inoculating other bacterial classification is lower.Experimental result shows, inoculate orange red newborn mushroom (Lactarius akahatsu), red juice breast mushroom (Lactarius hatsudake), pinnacle breast mushroom (Lactarius subdulcis) all has facilitation to the survival rate of China fir nursery stock respectively, is conducive to improve the validity of artificial afforestration.
The pinnacle breast mushroom (Lactarius subdulcis) that the Chinese fir woods is plucked or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) are cultivated through inclined-plane solid culture and liquid shaking table and obtain bacterium liquid.The solid culture condition: medium is PDA(20% potato juice 1000mL, glucose 18g, beef extract 15g, (NH
4)
2HPO
41g, magnesium sulfate 1.2g, agar 20g, vitaminB10 .01g); Cultivation temperature is 28 ℃; The pH scope is 5.8; Make medium and be used for primary inclined plane cultivation 7 days.Liquid culture condition: liquid nutrient medium PD medium; With the aseptic card punch of diameter 6mm, cut pinnacle breast mushroom or orange red newborn mushroom or the red juice breast mushroom bacterium sheet cultivated on the solid plate, be inoculated in the triangular flask (500ml) that fills the 250ml liquid nutrient medium 3 of every bottle graft kinds.Put 28 ℃, rotating speed 200rmin
– 17d is cultivated in water bath with thermostatic control on the shaking table, obtains bacterium liquid.Obtain carrying out bush mycorrhiza agent production behind the bacterium liquid.
It is the above-mentioned pinnacle breast mushroom (Lactarius subdulcis) that obtains or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) liquid spawn that bush mycorrhiza agent is produced used bacterial classification.Microbial inoculum solid fermentation medium is the peat composed of rotten mosses: wheat bran: corn flour: sucrose=20:15:10:1.Inoculate above-mentioned bacterium liquid, inoculum concentration is 5% of fermentation medium volume, and the humidity of matrix grabs with hand that to pinch sense moistening and do not have ponding and flow down to suitable, and producing used temperature is 28 ℃, and 7 days is one-period.
With after producing good bush mycorrhiza agent and being crushed to the 2mm size, methyl is dissolved as the liquid of 900ppm, then in bush mycorrhiza agent: the ratio of naphthalene acid solution=1000:1 fully mixes, and namely gets the degeneration-resistant health care bush mycorrhiza agent of Chinese fir.
Embodiment 2
The product using method:
When (1) the Chinese fir seedling is afforested, after fully mixing, the ratio of 1:1.5 adds suitable quantity of water (1 kilogram of degeneration-resistant health care bush mycorrhiza agent: 40 kg water) make mud with timbered soil again with the degeneration-resistant health care bush mycorrhiza agent of the present invention (culture of pinnacle breast mushroom solid fermentation is prepared from), the Chinese fir seedlings root is dipped in slurry, and mud can be bonded on the root system and get final product;
When (2) Chinese fir manually grows into forest, the polyacrylamide type water-loss reducer that adds 25g in the degeneration-resistant health care bush mycorrhiza agent of every 225g the present invention (mixture of the culture of pinnacle breast mushroom and orange red newborn mushroom and red juice breast mushroom solid fermentation is prepared from), after fully mixing, digging pit to imbed in the soil of about 1 meter of distance tree body gets final product.
Product seals preservation at normal temperatures, and the shelf-life is more than 1 year.
The using method of employing mode (1) is dipped in the cultivation of slurry back with the Chinese fir seedlings root, the Chinese fir seedling of contrast (CK) for not using degeneration-resistant health care bush mycorrhiza agent to handle.After 100 days, Chinese fir seedling growth indexes and disease resistance thereof are measured.The results are shown in Table 1.
The degeneration-resistant health care bush mycorrhiza agent of table 1 is to the influence of the growth of Chinese fir seedling and disease resistance
Result of the test shows, inoculates degeneration-resistant health care bush mycorrhiza agent and makes the Chinese fir height of seedling improve 19.2%, and diameter stem has improved 23.4%; The damping off incidence has reduced 24.1%, and the pestalotiopsis funerea incidence has reduced 28.38%, and survival rate has improved 22.85%.Illustrate that this degeneration-resistant health care bush mycorrhiza agent can promote the growth of Chinese fir seedling, reduce the incidence of nursery stock damping off and pestalotiopsis funerea, improve survival rate.
The using method of employing mode (2) is applied to 20 years living Chinese firs with degeneration-resistant health care bush mycorrhiza agent, and four different directions are digged pit in the soil of about 1 meter of distance tree body, and the degree of depth in hole is 25cm, and the amount that bury in each hole is about 250 grams, imbeds, and earthing, waters; The 20 year living Chinese firs of contrast (CK) for not using degeneration-resistant health care bush mycorrhiza agent.After 9 months, Chinese fir growth indexes and disease resistance are measured.The results are shown in Table 2
The degeneration-resistant health care bush mycorrhiza agent of table 2 is to the influence of living Chinese fir growth in 20 years and disease resistance
Result of the test shows, inoculates degeneration-resistant health care bush mycorrhiza agent and makes 20 years living Chinese fir height of tree increments improve 52.12%, and the diameter stem increment has improved 47.56%; The yellows incidence has reduced 26.36%.Illustrate that this degeneration-resistant health care bush mycorrhiza agent can promote living Chinese fir growth in 20 years, reduces the incidence of yellows.
Adopt the degeneration-resistant health care bush mycorrhiza agent of the culture preparation of orange red newborn mushroom or red juice breast mushroom solid fermentation separately, when adopting above-mentioned occupation mode to use, also can reach above-mentioned similar excellent effect.
Claims (7)
1. the degeneration-resistant health care bush mycorrhiza agent of a Chinese fir is characterized in that, by bush mycorrhiza agent: naphthalene acid solution is formulated according to weight ratio=800-1000:1; Described bush mycorrhiza agent is that pinnacle breast mushroom (Lactarius subdulcis) and orange red newborn mushroom (Lactarius akahatsu) and the newborn mushroom of red juice (Lactarius hatsudake) are adopted ferment in the culture that forms one or more of solid fermentation medium respectively; Described naphthalene acid solution concentration is 900ppm;
Before solid fermentation is cultivated, earlier pinnacle breast mushroom (Lactarius subdulcis) or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) are obtained bacterium liquid through inclined-plane solid culture medium and liquid shaking table medium culture, be inoculated into then in the solid fermentation medium and cultivate; Described solid fermentation culture medium prescription is: the peat composed of rotten mosses: wheat bran: corn flour: sucrose is pressed mass ratio=20-30:10-15:5-10:1; Bacterium liquid inoculum concentration is the 4-6% of solid fermentation culture volume, and fermentation temperature is 25 ℃-28 ℃, fermentation time 7-12 days;
Described liquid shaking table condition of culture: liquid nutrient medium adopts the PD medium that does not add agar; Aseptic card punch with diameter 6mm, cut pinnacle breast mushroom (Lactarius subdulcis) or orange red newborn mushroom (Lactarius akahatsu) or red juice breast mushroom (Lactarius hatsudake) the bacterium sheet cultivated on the inclined-plane solid plate, be inoculated in the 500ml triangular flask that fills the 250ml liquid nutrient medium every bottle graft kind 3-5 sheet; Put 25 ℃-28 ℃, rotating speed 200-220rmin
– 17-10d is cultivated in water bath with thermostatic control on the shaking table, obtains bacterium liquid;
Described pinnacle breast mushroom (Lactarius subdulcis), orange red newborn mushroom (Lactarius akahatsu), red juice breast mushroom (Lactarius hatsudake) are all picked up from the Chinese fir woods.
2. degeneration-resistant health care bush mycorrhiza agent according to claim 1 is characterized in that, mixes with naphthalene acid solution after bush mycorrhiza agent is crushed to the 2-3mm size again, namely obtains degeneration-resistant health care bush mycorrhiza agent.
3. degeneration-resistant health care bush mycorrhiza agent according to claim 1 is characterized in that, described inclined-plane solid culture condition: adopt the PDA medium: 20wt% potato juice 1000mL, glucose 18g, beef extract 15g, (NH
4)
2HPO
41g, magnesium sulfate 1.2g, agar 20g, vitaminB10 .01g; The cultivation temperature scope is 25 ℃-28 ℃; The pH scope is 5-6; Make medium and be used for primary inclined plane cultivation 7-10 days.
4. the application method of each described degeneration-resistant health care bush mycorrhiza agent of claim 1-3, it is characterized in that, be applied in the soil of Chinese fir tree root or plantation Chinese fir, promote the growth of Chinese fir, improve Chinese fir resistance, thereby strengthen the ability of Chinese fir resisting etiolation disease and China fir seedling damping off and pestalotiopsis funerea, improve survival rate and the growth rate of Chinese fir.
5. application method according to claim 4, it is characterized in that, specifically be when the Chinese fir seedling is afforested, after the ratio of 1:1.5 is fully mixed by weight with timbered soil with described degeneration-resistant health care bush mycorrhiza agent, again according to 1 kilogram of degeneration-resistant health care bush mycorrhiza agent: the ratio of 40 kg water adds water makes mud, the Chinese fir seedlings root is dipped in slurry, then plantation.
6. application method according to claim 4, it is characterized in that, specifically be when Chinese fir manually grows into forest, and adds the polyacrylamide type water-loss reducer of 25g in the described degeneration-resistant health care bush mycorrhiza agent of every 225g, after fully mixing, digging pit to imbed in the soil of 1 meter of distance tree body gets final product.
7. application method according to claim 6 is characterized in that, four different directions are digged pit in the soil of 1 meter of distance tree body, and the degree of depth in hole is 25cm, and the amount that bury in each hole is 250 grams.
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| CN111448947B (en) * | 2019-10-15 | 2022-05-03 | 遵义市林业科学研究所 | Preparation and sowing method of ectomycorrhizal fungi granule strain |
| CN114304159A (en) * | 2021-12-28 | 2022-04-12 | 石河子大学 | Medicament for improving stress resistance of desert oasis protection forest in western arid region |
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| 吴兴亮等.455 橙黄真菌.《中国热带真菌》.2011,(第1版),第436页. * |
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| 外生菌根与植物营养;周崇莲等;《生态学杂志》;19931231;第12卷(第1期);第37-44页 * |
| 红汁乳菇及人工合成菌根的形态结构特征;谭著明等;《食用菌学报》;20061231;第13卷(第2期);第36-40页 * |
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