CN108627548A - The environmental monitoring system and method for Animal Lab. - Google Patents

The environmental monitoring system and method for Animal Lab. Download PDF

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Publication number
CN108627548A
CN108627548A CN201710159594.4A CN201710159594A CN108627548A CN 108627548 A CN108627548 A CN 108627548A CN 201710159594 A CN201710159594 A CN 201710159594A CN 108627548 A CN108627548 A CN 108627548A
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CN
China
Prior art keywords
detecting device
machine detecting
ammonia
data
upper computer
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CN201710159594.4A
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Chinese (zh)
Inventor
赵勇
范春
王思远
郭佳伟
吴海卿
吴维华
陈国强
田立立
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SHANGHAI LAB ANIMAL RESEARCH CENTER
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SHANGHAI LAB ANIMAL RESEARCH CENTER
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Priority to CN201710159594.4A priority Critical patent/CN108627548A/en
Publication of CN108627548A publication Critical patent/CN108627548A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/12Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a solid body in dependence upon absorption of a fluid; of a solid body in dependence upon reaction with a fluid, for detecting components in the fluid
    • G01N27/125Composition of the body, e.g. the composition of its sensitive layer
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems

Abstract

The invention discloses the environmental monitoring system of Animal Lab. and method, which includes upper computer detection terminal, the next machine detecting device, router.The next machine detecting device is set in Animal Lab., and long-range, upper computer detection terminal and bottom machine detecting device are connected the setting of upper computer detection terminal by router.Upper computer detection terminal includes sensing chamber's terminal, database, external equipment, and the next machine detecting device includes sensing module, main control module, communication module.Sensing module collects multigroup sensing data and is simultaneously transmitted to main control module, and main control module, which integrates multigroup sensing data, generates control signal, is exchanged with router data by communication module, and router is by control signal transmission to sensing chamber's terminal.The environmental monitoring system and method for the Animal Lab. of the present invention can be applied to the measurement of the laboratory ammonia concentration under the disinfection situation of laboratory.

Description

The environmental monitoring system and method for Animal Lab.
Technical field
The present invention relates to a kind of laboratory environments to monitor system and method, more specifically to a kind of Animal Lab. Environmental monitoring system and method.
Background technology
Experimental animal grows in artificial environment, breeds and reception test processing, and particular place and its surrounding space are real Animal environment is tested, in biomedical research, accurate, authentic experiments data is obtained, to avoid physiology of the environmental factor to animal Biochemical activity adversely affect, create a good experimental animal environment, every monitoring index to animal living environment play to Close important role.Particularly important is exactly air purity and Particulate Pollution;Air purity index is directed to animal feeding Chamber interior air floating particulate matter (microorganism is more to be attached on particulate matter) and pernicious gas, main source are animal excrements Fermentation is decomposed.Wherein decomposition product includes ammonia, methyl mercaptan.Hydrogen sulfide, styrene, acetaldehyde etc..The rising of animal feeding environment temperature, Stocking density increases, ventilation condition is bad, excreta and the non-timely processing of bedding and padding can all cause ammonia density to steeply rise.And ammonia is made Animal eye conjunctiva can be stimulated when being increased for pungent concentration.Nasal membrane and respiratory mucosa cause to shed tears, cough.Seriously Person generates acute pulmonary edema and causes animal dead.It is chronically at the animal upper respiratory tract under high strength ammonia environment and chronic inflammation occurs, Seriously affect animal health.Particulate matter index is different with particulate matter size, and there are two types of approach for particulate matter main source:It is outdoor empty Gas is dealt with improperly through filtering to be brought directly to and animal skin, scurf, feed and bedding and padding etc. are often carried by air-flow or animal raises It floats in air.Similar to the mankind for animal, what is be affected to it is that diameter is less than 5 μm of dust below, this The flotation time is long in air for class dust, is sucked through respiratory tract and reaches bronchiole with alveolar so as to cause breathing problem; Grain object impacts bigger as microbe carrier in addition to itself generates harmful effect to animal to animal health.
Laboratory Animal Facility is continuous operation, and various environmental factors have been among variation, it is also desirable to regular Monitoring and maintenance.Every environmental index requires very strict, various measuring devices and people in the residing experiment room of experimental animal simultaneously Member is required for the processing that carries out disinfection, traditional ammonia measuring apparatus can not be directly facing current disinfection demand before entering.
According to the experiment of the mechanisms such as Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences, Beijing Experimental Amimal Research Centre It is found in zooscopy:The type of China experimental animal is mostly mouse (rat, mouse, suslik, cavy, Immune deficient mice, disease mould Type mouse etc.), rabbit, chicken, dog, pig, pig (miniature pig etc.), tree shrew, monkey (macaque etc.) etc., such experimental animal all has fertility By force, the smaller feature of build.Laboratory Animal Facility is continuous operation simultaneously, and various environmental factors have been among variation, It is also required to regular monitoring and maintenance.To experimental animal environment and facility condition, there is standardized regulation in country, according to GB 14925-2010《Experimental animal environment and facility》Regulation, detection project include that temperature, relative humidity, air purity, ammonia are dense Degree, noise, illumination and ventilation volume etc..Therefore its experimental situation must be directed to different animals and be equipped with various criterion, to maximum limit Degree reduces influence of the experimental animal to experiment itself.
The chemical method of ammonia has sodium hypochlorite-salicylic acid spectrophotometry, nessler reagent light splitting in existing measurement air Photometry, indophenol blue reagent colorimetric method etc..The measurement of these chemical methodes has many advantages, such as that the response time is short, it is sensitive to measure, still Disadvantage is also quite apparent, and measurement method and operating procedure are complicated, needs the labware by profession, while can only also carry out Spot measurement, it is undesirable for detecting system for measuring.
Traditional electrochemical sensor is separated by one layer of electrolytic thin-membrane, and is connected to by a low-impedance external circuit. On induction electrode surface oxidation or reduction reaction occur for the gas for diffusing into sensor, and an internal electricity is generated between two electrodes Stream, current value correspond to oxygen concentration.However when tested gas concentration continues to increase, induction electrode electromotive force will be more than to allow Range, sensor output will become measurement concentration that is nonlinear, thus limiting gas.
Invention content
The problem of being not applied for test in laboratory for ammonia measurement method existing in the prior art, the purpose of the present invention It is to provide a kind of environmental monitoring system and method for Animal Lab..
To achieve the above object, the present invention adopts the following technical scheme that:
A kind of environmental monitoring system of Animal Lab., including upper computer detection terminal, the next machine detecting device, routing Device.The next machine detecting device is set in Animal Lab., the setting of upper computer detection terminal long-range, upper computer detection terminal and The next machine detecting device is connected by router.Upper computer detection terminal includes sensing chamber's terminal, database, external equipment, The next machine detecting device includes sensing module, main control module, communication module.Sensing module is collected multigroup sensing data and is transmitted To main control module, main control module, which integrates multigroup sensing data, generates control signal, passes through communication module and router data It exchanges, router is by control signal transmission to sensing chamber's terminal.
Further, sensing module includes ammonia gas sensor, temperature sensor, humidity sensor, particulate matter sensors.Ammonia Gas sensor, temperature sensor, particulate matter sensors and humidity sensor are respectively connected to main control module, and ammonia gas sensor includes Induction electrode, negative electrode and reference electrode, the external regulator circuit of reference electrode connection is to stablize the electromotive force of induction electrode.
Further, main control module includes multiple A/D interfaces, ammonia gas sensor output analog signal, and passes through master control mould Block is configured to 8 or 12 digit word formats.
Further, the gas sensitive of ammonia gas sensor is stannic oxide.
Further, ammonia gas sensor includes 4 pins, and signal is spread out of from 2,4 pins, and No. 4 leg signals are directly defeated Go out analog voltage signal, output digital voltage letter after No. 2 leg signals are then amplified by amplifier and divided by adjustable resistor Number.
To achieve the above object, the present invention also adopts the following technical scheme that:
A kind of method of environmental monitoring of Animal Lab., including upper computer detection terminal and the next machine detecting device, it is upper Machine testing terminal executes following steps:Start networking operation;Receive the data packet of the next machine detecting device;Data pack protocol turns It changes;Data carry out broken line and show;Data store;Judge whether to receive end signalIf it is not, continuing to the next machine testing The data packet of device;If so, terminating.
To achieve the above object, the present invention also adopts the following technical scheme that:
The next machine detecting device executes following steps:It is put into gas;Trigger ammonia collection event;Stablize ammonia gas sensor ginseng Examine the voltage of electrode and the electromotive force of induction electrode;Receive the letter of ammonia gas sensor, temperature sensor and humidity sensor Number;Ammonia concentration data are corrected according to the signal of temperature sensor and humidity sensor.
To achieve the above object, the present invention also adopts the following technical scheme that:
A kind of method of environmental monitoring of Animal Lab., including upper computer detection terminal and the next machine detecting device, it is special Sign is:Upper computer detection terminal executes following steps:Start networking operation;Receive the data packet of the next machine detecting device;Number It is converted according to packet protocol;Data carry out broken line and show;Data store;Judge whether to receive end signalIf it is not, continuing to The data packet of the next machine detecting device;If so, terminating.Meanwhile the next machine detecting device executes following steps:It is put into gas; Trigger ammonia collection event;Stablize the voltage of ammonia gas sensor reference electrode and the electromotive force of induction electrode;Ammonia is received to pass The signal of sensor, temperature sensor and humidity sensor;Ammonia is corrected according to the signal of temperature sensor and humidity sensor Concentration data.
Further, upper computer detection terminal judges ammonia concentration, temperature, humidity, judges whether ammonia integrated concentration surpasses Cross default warning value.
In the above-mentioned technical solutions, the environmental monitoring system of Animal Lab. of the invention and method can be applied to test The measurement of laboratory ammonia concentration under the disinfection situation of room.
Description of the drawings
Fig. 1 is the structural schematic diagram of environmental monitoring system of the present invention;
Fig. 2 is the circuit diagram of ammonia gas sensor and its peripheral circuit;
Fig. 3 is the method flow diagram that upper computer detection terminal executes;
Fig. 4 is the method flow diagram that the next machine detecting device executes.
Specific implementation mode
The technical solution further illustrated the present invention with reference to the accompanying drawings and examples.
Referring to Fig.1, the present invention discloses a kind of Portable environmental-monitoring system of Animal Lab. first, includes mainly upper Position machine testing terminal, the next machine detecting device and router 6.The next machine detecting device is set in Animal Lab., host computer Long-range, upper computer detection terminal and bottom machine detecting device are connected by router 6 for detection terminal setting.The present invention be System includes a upper computer detection terminal and multiple the next machine detecting devices, and a bottom can be arranged in each laboratory Machine detecting device, each bottom machine detecting device are connected respectively to by router 6 in upper computer detection terminal.
With continued reference to Fig. 1, upper computer detection terminal includes sensing chamber's terminal 11, database 9, external equipment 10, slave computer Detection device includes sensing module, main control module, communication module.Sensing module collects multigroup sensing data and is transmitted to master control Module, main control module, which integrates multigroup sensing data, generates control signal, is exchanged with router data by communication module, road By device by control signal transmission to sensing chamber's terminal.
Upper computer detection terminal is monitoring center, and the data that each experiment room node measures, packet are received by router 6 Ammonia concentration, humiture are included, throughput, illuminance and co2 concentration etc. make its line chart be shown in terminal by data processing On, while judging whether the data value of ammonia integrated concentration is more than setting warning line, and accordingly make alert process.
In the present invention, ammonia integrated concentration is concentration of ammonia under conditions of specific temperature, humidity, particle concentration Value, with reference to the particular condition under zoopery room environmental, not simply by ammonia measured data.
As shown in Figure 1, the next machine detecting device includes main control module 1, ammonia gas sensor 3, temperature sensor 4, humidity biography Sensor 5, particulate matter sensors 2, wireless communication module 8.Above-mentioned module is integrated in a shell, shell dimension 263*184* 95mm using plastics waterproofing material, while having high corrosion resistance, also avoids the signal shielding of metal shell, ensure that The normal transmission of signal.Air outlet valve uses plastic cable waterproof connector, waterproofing grade to can reach IP67.
Continue as shown in Figure 1, ammonia gas sensor 3, temperature sensor 4, humidity sensor 5 and particulate matter sensors 2 connect Main control module 1 is connect, ammonia gas sensor 3 includes induction electrode, negative electrode and reference electrode, the external regulator circuit of reference electrode connection To stablize the electromotive force of induction electrode.The signal of temperature sensor 4 and humidity sensor 5 is correcting ammonia concentration data.It is aobvious Show that module 7 is LCD module, is connected to monitoring room terminal 11, monitoring room terminal 11 is by ammonia concentration data transmission to showing mould Block 7.Wireless communication module 8 remotely obtains data for device, and since this portable unit portability is strong, mobility is big, then passes Defeated distance may be very big, and simultaneous transmission data are smaller, therefore have selected GPRS transmission mode, while using place in view of different Different wireless communication modules 8 can be added, such as WIFI module passes through router transmission, bluetooth module etc..
As a portable device, main control module 1 is set in a shell, particulate matter sensors 2, ammonia gas sensor 3, Temperature sensor 4 and humidity sensor 5 are set to the unidirectional side of shell.Since the device of the invention is real for animal It tests room to measure, object is the mixed gas with ammonia of the excreta release of animal, and such gaseous mixture scale of construction Less, it is unevenly distributed, there is directive property and regionality.Therefore, this have the advantage that particulate matter sensors 2, temperature by the present invention Degree sensor 4 and humidity sensor 5 and ammonia gas sensor 3 are substantial access to, and are more had in terms of the number of degrees of correction ammonia gas sensor 3 Profit.
In the present invention, main control module uses high performance ARMCortex- using STM32F103 microcontrollers as core M332 RISC cores, working frequency 72MHz, internal high-speed memories (the up to flash memory and 20K bytes of 128K bytes SRAM), the abundant enhancing ports I/O and the peripheral hardware for being connected to two APB buses.Device include 2 12 ADC, 3 it is general 16 bit timing devices and a PWM timer also include standard and advanced communication interface:Up to 2 I2C and SPI, 3 USART, USB and CAN.It ensure that the efficient conversion for 3 signal of ammonia gas sensor, while being also later stage module Addition and transplanting provide possibility.The DAC module (numeral/analog conversion module) of STM32 is the input of 12 bit digitals, and voltage is defeated Go out the DAC of type.The output of 3 module of ammonia gas sensor is exactly voltage analog signal, is connected to the arbitrary A/D pins of STM32F103 Afterwards, 8 or 12 bit patterns are configured, while input reference voltage VREF+ just obtains more accurate transformation result, that is, be converted into corresponding Digital signal.Its voltage, ammonia concentration curved line relation is recycled to obtain after obtaining 3 module carry-out bit voltage of ammonia gas sensor Function, can be obtained corresponding ammonia concentration data by conversion processing, and work is measured to complete ammonia concentration.
Other than STM32F103 main control modules, remaining module is all added on main control module 1, is controlled by main control module 1 The acquisition of pelleting object sensor 2, ammonia gas sensor 3 and Temperature Humidity Sensor data-signal, turns after program is handled later It is melted into ammonia concentration and data of the Temperature and Humidity module, and makes its display on the lcd screen, is carried out at the same time the storage of data.
With reference to Fig. 2, the ammonia gas sensor 3 in Fig. 1 uses the measurement method of three-electrode electro Chemical.Three electrode ammonias sense Device is by accessing external regulator circuit, and reference electrode to be added to stablize induction electrode on the basis of two electrode sensors Electromotive force.In this way, any influence will not be generated to induction electrode, negative electrode persistently polarizes so three electrode ammonias sense Utensil has wider array of measurement range.
As shown in Fig. 2, the energizations such as power supply instruction light after ammonia gas sensor (MQ sensors) energization, gas sensitive is clear The lower stannic oxide of conductivity in clean air, when sensor local environment is there are when ammonia, sensor conductance rate is with air Middle concentration increases and increases, and signal is spread out of from MQ sensors 2,4 pins, and No. 4 leg signals directly export analog voltage signal (AOUT), No. 2 leg signals are then amplified by amplifier LM393AD and are exported after being divided by adjustable resistor, i.e. digital voltage Signal (DOUT).Wherein adjusting and zeroing of the adjustable resistor effect for sensor assembly, is adjusted when in no ammonia environment Section knob makes alarm lamp be in bright with not bright limb;Since then indicator light is bright for voltage raising when there are ammonia environment measurement It rises.
In the present invention, electrochemical ammonia sensor ME3-NH3 works according to the principle of electrochemistry, is existed using under test gas Electrochemical oxidation process in electrolytic cell on working electrode, it is by electronic circuit that the working electrode of electrolytic cell and reference electrode is permanent It is scheduled on a current potential appropriate, the electrochemical oxidation of under test gas can occur under the current potential, since oxygen is being aoxidized and restored Generated faradic currents very little, can be ignored when reaction, then electric current caused by under test gas electrochemical reaction It is directly proportional to its concentration and follow Faraday's law.In this way, being assured that the dense of under test gas by the size for measuring electric current Degree.
With reference to Fig. 3 and Fig. 4, invention additionally discloses a kind of detection methods for above-mentioned apparatus comprising upper computer detection The step of the step of terminal executes and the next machine detecting device execute
As shown in figure 3, upper computer detection terminal executes key step below:
S11:Start networking operation.
S12:Whether wlan network connection is establishedIf it is not, S11 is returned to, if then continuing subsequent step.
S13:Judge whether to receive the next machine detecting device data packetIf then continuing subsequent step, if otherwise waiting for Data packet.
S14:Complete data pack protocol conversion.
S15:Data carry out broken line and show.
S16:Data store.
S17:Judge whether to receive end signalIf it is not, continuing to the data packet of the next machine detecting device;If so, Then terminate.
As shown in figure 4, the next machine detecting device executes key step below:
S1:System first carries out initialization detection after starting, and determines and just starts to survey after sensor assembly and other modules are normal Amount prepares.
S2:Start detection operation.
S3:Judge whether to add wireless network moduleIf so, continuing subsequent step, if it is not, returning to S2.
S4:It waits for carrying out disinfection processing (spray or immersing type disinfection) according to Laboratory Request after the completion of gas pumping.
S5:Experiment room to be measured is put into after disinfection completely, that is, is put into laboratory under test gas, since ambient pressure is more than device Interior air pressure can be then full of inside device by gas.
S6:At this point, judging whether to trigger ammonia gas sensor measurement eventIf so, into S8, if it is not, then entering S7.
S7:Etc. ammonia gas sensor measurement event to be triggered, and return to S6.
S8:It is converted to ammonia concentration through measuring circuit data measured and by function program, if it is more than to set to measure gas Determine zoopery room ammonia standard and then carries out sound-light alarm
S9:LCD shows that data are then constantly sent to client by ammonia concentration data, wireless sensing device (i.e. GPRS module) It holds, constantly cycle measures until closing measurement after data measured.
After measurement, device internal gas is extracted out, the influence to avoid irritant gas to internal sensor is dropped simultaneously The low previous influence for measuring gas and next time being measured.So far it measures and terminates.
Those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate the present invention, And be not used as limitation of the invention, as long as in the spirit of the present invention, the change to embodiment described above Change, modification will all be fallen within the scope of claims of the present invention.

Claims (9)

1. a kind of environmental monitoring system of Animal Lab., which is characterized in that including:
Upper computer detection terminal, the next machine detecting device, router;
The bottom machine detecting device is set in Animal Lab., upper computer detection terminal setting long-range, it is described on Position machine testing terminal is connected with the next machine detecting device by router;
The upper computer detection terminal includes sensing chamber's terminal, database, external equipment;
The bottom machine detecting device includes sensing module, main control module, communication module;
The sensing module collects multigroup sensing data and is transmitted to main control module, and the main control module is by multigroup sensor number Control signal is generated according to integrating, is exchanged with router data by communication module, the router is by control signal transmission to institute State sensing chamber's terminal.
2. the environmental monitoring system of Animal Lab. as described in claim 1, which is characterized in that the sensing module includes:
Ammonia gas sensor, temperature sensor, humidity sensor, particulate matter sensors;
The ammonia gas sensor, temperature sensor, particulate matter sensors and humidity sensor are respectively connected to the main control module;
The ammonia gas sensor includes induction electrode, negative electrode and reference electrode, the external regulator circuit of reference electrode connection To stablize the electromotive force of induction electrode.
3. the environmental monitoring system of Animal Lab. as claimed in claim 2, which is characterized in that the main control module includes more A A/D interfaces, the ammonia gas sensor export analog signal, and are configured to 8 or 12 digit word formats by main control module.
4. the environmental monitoring system of Animal Lab. as described in claim 1, which is characterized in that the gas of the ammonia gas sensor Quick material is stannic oxide.
5. the environmental monitoring system of Animal Lab. as described in claim 1, which is characterized in that the ammonia gas sensor includes 4 pins, signal are spread out of from 2,4 pins, and No. 4 leg signals directly export analog voltage signal, and No. 2 leg signals then pass through Amplifier amplifies and exports digital voltage signal after being divided by adjustable resistor.
6. a kind of method of environmental monitoring of Animal Lab., including upper computer detection terminal and the next machine detecting device, feature It is, the upper computer detection terminal executes following steps:
Start networking operation;
Receive the data packet of the next machine detecting device;
Data pack protocol is converted;
Data carry out broken line and show;
Data store;
Judge whether to receive end signalIf it is not, continuing to the data packet of the next machine detecting device;If so, terminating.
7. a kind of method of environmental monitoring of Animal Lab., including upper computer detection terminal and the next machine detecting device, feature It is, the bottom machine detecting device executes following steps:
It is put into gas;
Trigger ammonia collection event;
Stablize the voltage of ammonia gas sensor reference electrode and the electromotive force of induction electrode;
Receive the signal of ammonia gas sensor, temperature sensor and humidity sensor;
Ammonia concentration data are corrected according to the signal of temperature sensor and humidity sensor.
8. a kind of method of environmental monitoring of Animal Lab., including upper computer detection terminal and the next machine detecting device, feature It is:
Upper computer detection terminal executes following steps:
Start networking operation;
Receive the data packet of the next machine detecting device;
Data pack protocol is converted;
Data carry out broken line and show;
Data store;
Judge whether to receive end signalIf it is not, continuing to the data packet of the next machine detecting device;If so, terminating;
Meanwhile the next machine detecting device executes following steps:
It is put into gas;
Trigger ammonia collection event;
Stablize the voltage of ammonia gas sensor reference electrode and the electromotive force of induction electrode;
Receive the signal of ammonia gas sensor, temperature sensor and humidity sensor;
Ammonia concentration data are corrected according to the signal of temperature sensor and humidity sensor.
9. the method for environmental monitoring of Animal Lab. as claimed in claim 8, which is characterized in that the upper computer detection terminal Judge ammonia concentration, temperature, humidity, judges whether ammonia integrated concentration is more than default warning value.
CN201710159594.4A 2017-03-17 2017-03-17 The environmental monitoring system and method for Animal Lab. Pending CN108627548A (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN201710159594.4A CN108627548A (en) 2017-03-17 2017-03-17 The environmental monitoring system and method for Animal Lab.

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Application publication date: 20181009

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