CN108572001A - The Portable environmental-monitoring device and method of Animal Lab. - Google Patents
The Portable environmental-monitoring device and method of Animal Lab. Download PDFInfo
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- CN108572001A CN108572001A CN201710164462.0A CN201710164462A CN108572001A CN 108572001 A CN108572001 A CN 108572001A CN 201710164462 A CN201710164462 A CN 201710164462A CN 108572001 A CN108572001 A CN 108572001A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
Abstract
The invention discloses a kind of Portable environmental-monitoring device and method of Animal Lab., which includes main control module, ammonia gas sensor, temperature sensor, humidity sensor, sensor adapter.Ammonia gas sensor, temperature sensor and humidity sensor are respectively connected to sensor adapter, sensor adapter connects main control module, ammonia gas sensor includes induction electrode, negative electrode and reference electrode, and the external regulator circuit of reference electrode connection is to stablize the electromotive force of induction electrode.The signal of temperature sensor and humidity sensor is correcting ammonia concentration data.The Portable environmental-monitoring device and method of the Animal Lab. of the present invention can be applied to the measurement of the laboratory ammonia concentration under the disinfection situation of laboratory.
Description
Technical field
The present invention relates to laboratory environment monitoring device and its methods, more specifically to a kind of Animal Lab.
Portable environmental-monitoring device and method.
Background technology
Experimental animal grows in artificial environment, breeds and reception test processing, and particular place and its surrounding space are real
Animal environment is tested, in biomedical research, accurate, authentic experiments data is obtained, to avoid physiology of the environmental factor to animal
Biochemical activity adversely affect, create a good experimental animal environment, every monitoring index to animal living environment play to
Close important role.Particularly important is exactly air purity and Particulate Pollution；Air purity index is directed to animal feeding
Chamber interior air floating particulate matter (microorganism is more to be attached on particulate matter) and pernicious gas, main source are animal excrements
Fermentation is decomposed.Wherein decomposition product includes ammonia, methyl mercaptan.Hydrogen sulfide, styrene, acetaldehyde etc..The rising of animal feeding environment temperature,
Stocking density increases, ventilation condition is bad, excreta and the non-timely processing of bedding and padding can all cause ammonia density to steeply rise.And ammonia is made
Animal eye conjunctiva can be stimulated when being increased for pungent concentration.Nasal membrane and respiratory mucosa cause to shed tears, cough.Seriously
Person generates acute pulmonary edema and causes animal dead.It is chronically at the animal upper respiratory tract under high strength ammonia environment and chronic inflammation occurs,
Seriously affect animal health.Particulate matter index is different with particulate matter size, and there are two types of approach for particulate matter main source：It is outdoor empty
Gas is dealt with improperly through filtering to be brought directly to and animal skin, scurf, feed and bedding and padding etc. are often carried by air-flow or animal raises
It floats in air.Similar to the mankind for animal, what is be affected to it is that diameter is less than 5 μm of dust below, this
The flotation time is long in air for class dust, is sucked through respiratory tract and reaches bronchiole with alveolar so as to cause breathing problem；
Grain object impacts bigger as microbe carrier in addition to itself generates harmful effect to animal to animal health.
Laboratory Animal Facility is continuous operation, and various environmental factors have been among variation, it is also desirable to regular
Monitoring and maintenance.Every environmental index requires very strict, various measuring devices and people in the residing experiment room of experimental animal simultaneously
Member is required for the processing that carries out disinfection, traditional ammonia measuring apparatus can not be directly facing current disinfection demand before entering.
According to the experiment of the mechanisms such as Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences, Beijing Experimental Amimal Research Centre
It is found in zooscopy：The type of China experimental animal is mostly mouse (rat, mouse, suslik, cavy, Immune deficient mice, disease mould
Type mouse etc.), rabbit, chicken, dog, pig, pig (miniature pig etc.), tree shrew, monkey (macaque etc.) etc., such experimental animal all has fertility
By force, the smaller feature of build.Laboratory Animal Facility is continuous operation simultaneously, and various environmental factors have been among variation,
It is also required to regular monitoring and maintenance.To experimental animal environment and facility condition, there is standardized regulation in country, according to GB
14925-2010《Experimental animal environment and facility》Regulation, detection project include that temperature, relative humidity, air purity, ammonia are dense
Degree, noise, illumination and ventilation volume etc..Therefore its experimental situation must be directed to different animals and be equipped with various criterion, to maximum limit
Degree reduces influence of the experimental animal to experiment itself.
The chemical method of ammonia has sodium hypochlorite-salicylic acid spectrophotometry, nessler reagent light splitting in existing measurement air
Photometry, indophenol blue reagent colorimetric method etc..The measurement of these chemical methodes has many advantages, such as that the response time is short, it is sensitive to measure, still
Disadvantage is also quite apparent, and measurement method and operating procedure are complicated, needs the labware by profession, while can only also carry out
Spot measurement, it is undesirable for detecting system for measuring.
Traditional electrochemical sensor is separated by one layer of electrolytic thin-membrane, and is connected to by a low-impedance external circuit.
On induction electrode surface oxidation or reduction reaction occur for the gas for diffusing into sensor, and an internal electricity is generated between two electrodes
Stream, current value correspond to oxygen concentration.However when tested gas concentration continues to increase, induction electrode electromotive force will be more than to allow
Range, sensor output will become measurement concentration that is nonlinear, thus limiting gas.
Invention content
The problem of being not applied for test in laboratory for ammonia measurement method existing in the prior art, the purpose of the present invention
It is to provide a kind of Portable environmental-monitoring device and method of Animal Lab..
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of Portable environmental-monitoring device of Animal Lab., including main control module, ammonia gas sensor, temperature sensing
Device, humidity sensor, sensor adapter.Ammonia gas sensor, temperature sensor and humidity sensor are respectively connected to sensor
Adapter, sensor adapter connect main control module, and ammonia gas sensor includes induction electrode, negative electrode and reference electrode, reference
The external regulator circuit of electrode connection is to stablize the electromotive force of induction electrode.The signal of temperature sensor and humidity sensor is to strong
Positive ammonia concentration data.
Further, main control module includes multiple A/D interfaces, ammonia gas sensor output analog signal, and passes through master control mould
Block is configured to 8 or 12 digit word formats.
Further, further include display module, display module is connected to main control module, and main control module is by ammonia concentration data
It is transmitted to display module.
Further, sensor adapter, main control module are set in a shell, ammonia gas sensor, temperature sensor and
Humidity sensor is set to the unidirectional side of shell.
Further, the gas sensitive of ammonia gas sensor is stannic oxide.
Further, ammonia gas sensor includes 4 pins, and signal is spread out of from 2,4 pins, and No. 4 leg signals are directly defeated
Go out analog voltage signal, output digital voltage letter after No. 2 leg signals are then amplified by amplifier and divided by adjustable resistor
Number.
To achieve the above object, the present invention also adopts the following technical scheme that：
A kind of method of environmental monitoring of Animal Lab., includes the following steps：It opens solenoid valve and is put into gas；Trigger ammonia
Collection event；Stablize the voltage of ammonia gas sensor reference electrode and the electromotive force of induction electrode；Receive ammonia gas sensor, temperature
Spend the signal of sensor and humidity sensor；Ammonia concentration number is corrected according to the signal of temperature sensor and humidity sensor
According to.
Further, further include pre-treatment step：Starter is evacuated and opens solenoid valve；Pumping is completed to close electromagnetism
Valve, and disinfected.
In the above-mentioned technical solutions, the Portable environmental-monitoring device and method of Animal Lab. of the invention can be applied
The measurement of laboratory ammonia concentration under the disinfection situation of laboratory.
Description of the drawings
Fig. 1 is the structural schematic diagram of environment monitoring device of the present invention；
Fig. 2 is the circuit diagram of ammonia gas sensor and its peripheral circuit；
Fig. 3 is the flow chart of method of environmental monitoring of the present invention.
Specific implementation mode
The technical solution further illustrated the present invention with reference to the accompanying drawings and examples.
Referring to Fig.1, the present invention discloses a kind of Portable environmental-monitoring device of Animal Lab., main modular packet first
Include main control module 1, ammonia gas sensor 3, temperature sensor 4, humidity sensor 5, sensor adapter 2, memory module 6, display
Module 7, wireless communication module 8.Above-mentioned module is integrated in a shell, shell dimension 263*184*95mm, anti-using plastics
Water material, while there is high corrosion resistance, the signal shielding of metal shell is also avoided, ensure that the normal transmission of signal.
Air outlet valve uses plastic cable waterproof connector, waterproofing grade to can reach IP67.
As shown in Figure 1, ammonia gas sensor 3, temperature sensor 4 and humidity sensor 5 are respectively connected to sensor adapter
2, sensor adapter 2 connects main control module 1, and ammonia gas sensor 3 includes induction electrode, negative electrode and reference electrode, with reference to electricity
Connection external regulator circuit in pole is to stablize the electromotive force of induction electrode.The signal of temperature sensor 4 and humidity sensor 5 is to strong
Positive ammonia concentration data.Display module 7 is LCD module, is connected to main control module 1, main control module 1 passes ammonia concentration data
Transport to display module 7.Wireless communication module 8 remotely obtains data for device, since this portable unit portability is strong, flowing
Property it is big, then transmission range may be very big, and simultaneous transmission data are smaller, therefore have selected GPRS transmission mode, while in view of difference
Different wireless communication modules 8 can be added with place, such as WIFI module passes through router transmission, bluetooth module etc.
Deng.
As a portable device, sensor adapter 2, main control module 1 may be contained in a shell, ammonia gas sensor
3, temperature sensor 4 and humidity sensor 5 are set to the unidirectional side of shell.Since the device of the invention is directed to animal
Laboratory measures, and object is the mixed gas with ammonia of the excreta release of animal, and such mixed gas
It measures less, be unevenly distributed, there is directive property and regionality.Therefore, this have the advantage that 4 Hes of temperature sensor by the present invention
Humidity sensor 5 and ammonia gas sensor 3 are substantial access to, in terms of the number of degrees of correction ammonia gas sensor 3 advantageously.
In the present invention, main control module uses high performance ARMCortex- using STM32F103 microcontrollers as core
M332 RISC cores, working frequency 72MHz, internal high-speed memories (the up to flash memory and 20K bytes of 128K bytes
SRAM), the abundant enhancing ports I/O and the peripheral hardware for being connected to two APB buses.Device include 2 12 ADC, 3 it is general
16 bit timing devices and a PWM timer also include standard and advanced communication interface：Up to 2 I2C and SPI, 3
USART, USB and CAN.It ensure that the efficient conversion for 3 signal of ammonia gas sensor, while being also later stage module
Addition and transplanting provide possibility.The DAC module (numeral/analog conversion module) of STM32 is the input of 12 bit digitals, and voltage is defeated
Go out the DAC of type.The output of 3 module of ammonia gas sensor is exactly voltage analog signal, is connected to the arbitrary A/D pins of STM32F103
Afterwards, 8 or 12 bit patterns are configured, while input reference voltage VREF+ just obtains more accurate transformation result, that is, be converted into corresponding
Digital signal.Its voltage, ammonia concentration curved line relation is recycled to obtain after obtaining 3 module carry-out bit voltage of ammonia gas sensor
Function, can be obtained corresponding ammonia concentration data by conversion processing, and work is measured to complete ammonia concentration.
Other than STM32F103 main control modules, remaining module is all added on main control module 1, is controlled by main control module 1
The acquisition of ammonia gas sensor 3 and Temperature Humidity Sensor data-signal processed,；Be converted to after program is handled later ammonia concentration and
Data of the Temperature and Humidity module, and make its display on the lcd screen, it is carried out at the same time the storage of data.
With reference to Fig. 2, the ammonia gas sensor 3 in Fig. 1 uses the measurement method of three-electrode electro Chemical.Three electrode ammonias sense
Device is by accessing external regulator circuit, and reference electrode to be added to stablize induction electrode on the basis of two electrode sensors
Electromotive force.In this way, any influence will not be generated to induction electrode, negative electrode persistently polarizes so three electrode ammonias sense
Utensil has wider array of measurement range.
As shown in Fig. 2, the energizations such as power supply instruction light after ammonia gas sensor (MQ sensors) energization, gas sensitive is clear
The lower stannic oxide of conductivity in clean air, when sensor local environment is there are when ammonia, sensor conductance rate is with air
Middle concentration increases and increases, and signal is spread out of from MQ sensors 2,4 pins, and No. 4 leg signals directly export analog voltage signal
(AOUT), No. 2 leg signals are then amplified by amplifier LM393AD and are exported after being divided by adjustable resistor, i.e. digital voltage
Signal (DOUT).Wherein adjusting and zeroing of the adjustable resistor effect for sensor assembly, is adjusted when in no ammonia environment
Section knob makes alarm lamp be in bright with not bright limb；Since then indicator light is bright for voltage raising when there are ammonia environment measurement
It rises.
In the present invention, electrochemical ammonia sensor ME3-NH3 works according to the principle of electrochemistry, is existed using under test gas
Electrochemical oxidation process in electrolytic cell on working electrode, it is by electronic circuit that the working electrode of electrolytic cell and reference electrode is permanent
It is scheduled on a current potential appropriate, the electrochemical oxidation of under test gas can occur under the current potential, since oxygen is being aoxidized and restored
Generated faradic currents very little, can be ignored when reaction, then electric current caused by under test gas electrochemical reaction
It is directly proportional to its concentration and follow Faraday's law.In this way, being assured that the dense of under test gas by the size for measuring electric current
Degree.
With reference to Fig. 3, invention additionally discloses a kind of detection methods for above-mentioned apparatus comprising key step below：
S1：System first carries out initialization detection after starting, and determines and just starts to survey after sensor assembly and other modules are normal
Amount prepares.
S2：Start electronic valve and is evacuated.Specifically, it opens solenoid valve and opens exhaust fan pumping, waiting for will be inside device
Solenoid valve is closed in gas pumping when complete, forming class vacuum state inside device (is actually unable to reach vacuum, but will portion big absolutely
Divide gas extraction).
S3：Judge whether to add wireless network moduleIf so, continuing subsequent step, if it is not, returning to S2.
S4：It waits for carrying out disinfection processing (spray or immersing type disinfection) according to Laboratory Request after the completion of gas pumping.
S5：Experiment room to be measured is put into after disinfection completely, that is, is put into laboratory under test gas environment and is opened solenoid valve, due to
Ambient pressure is more than air pressure in device, then can be full of by gas inside device.
S6：At this point, judging whether to trigger ammonia gas sensor measurement eventIf so, into S8, if it is not, then entering S7.
S7：Etc. ammonia gas sensor measurement event to be triggered, and return to S6.
S8：It is converted to ammonia concentration through measuring circuit data measured and by function program, if it is more than to set to measure gas
Determine zoopery room ammonia standard and then carries out sound-light alarm
S9：LCD shows that data are then constantly sent to client by ammonia concentration data, wireless sensing device (i.e. GPRS module)
It holds, constantly cycle measures until closing measurement after data measured.
After measurement, device internal gas is extracted out, the influence to avoid irritant gas to internal sensor is dropped simultaneously
The low previous influence for measuring gas and next time being measured.So far it measures and terminates.
Those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate the present invention,
And be not used as limitation of the invention, as long as in the spirit of the present invention, the change to embodiment described above
Change, modification will all be fallen within the scope of claims of the present invention.
Claims (8)
1. a kind of Portable environmental-monitoring device of Animal Lab., which is characterized in that including：
Main control module, ammonia gas sensor, temperature sensor, humidity sensor, sensor adapter；
The ammonia gas sensor, temperature sensor and humidity sensor are respectively connected to the sensor adapter, the sensing
Device adapter connects the main control module；
The ammonia gas sensor includes induction electrode, negative electrode and reference electrode, the external regulator circuit of reference electrode connection
To stablize the electromotive force of induction electrode；
The signal of the temperature sensor and humidity sensor is correcting ammonia concentration data.
2. the Portable environmental-monitoring device of Animal Lab. as described in claim 1, which is characterized in that the main control module
Analog signal is exported, and 8 or 12 bit digitals are configured to by main control module including multiple A/D interfaces, the ammonia gas sensor
Format.
3. the Portable environmental-monitoring device of Animal Lab. as described in claim 1, which is characterized in that further include display mould
Block, the display module are connected to the main control module, and the main control module is by ammonia concentration data transmission to the display mould
Block.
4. the Portable environmental-monitoring device of Animal Lab. as described in claim 1, which is characterized in that the sensor is suitable
Orchestration, main control module are set in a shell, and the ammonia gas sensor, temperature sensor and humidity sensor are set to shell
Unidirectional side.
5. the Portable environmental-monitoring device of Animal Lab. as described in claim 1, which is characterized in that the ammonia sensing
The gas sensitive of device is stannic oxide.
6. the Portable environmental-monitoring device of Animal Lab. as described in claim 1, which is characterized in that the ammonia sensing
Device includes 4 pins, and signal is spread out of from 2,4 pins, and No. 4 leg signals directly export analog voltage signal, No. 2 leg signals
Then amplified by amplifier and exports digital voltage signal after being divided by adjustable resistor.
7. a kind of method of environmental monitoring of Animal Lab., which is characterized in that include the following steps：
It opens solenoid valve and is put into gas；
Trigger ammonia collection event；
Stablize the voltage of ammonia gas sensor reference electrode and the electromotive force of induction electrode；
Receive the signal of ammonia gas sensor, temperature sensor and humidity sensor；
Ammonia concentration data are corrected according to the signal of temperature sensor and humidity sensor.
8. the method for environmental monitoring of Animal Lab. as claimed in claim 7, which is characterized in that further include pre-treatment step：
Starter is evacuated and opens solenoid valve；
Pumping is completed to close solenoid valve, and is disinfected.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114755271A (en) * | 2022-04-15 | 2022-07-15 | 四川大学 | Electronic nose system for detecting multiple volatile organic compounds |
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