CN206627150U - The Portable environmental-monitoring device of Animal Lab. - Google Patents

Abstract

The utility model discloses a kind of Portable environmental-monitoring device of Animal Lab., the device includes main control module, ammonia gas sensor, temperature sensor, humidity sensor, sensor adapter.Ammonia gas sensor, temperature sensor and humidity sensor are respectively connecting to sensor adapter, sensor adapter connects main control module, ammonia gas sensor includes induction electrode, negative electrode and reference electrode, and reference electrode connects outside mu balanced circuit to stablize the electromotive force of induction electrode.The signal of temperature sensor and humidity sensor is correcting ammonia concentration data.The Portable environmental-monitoring device and method of Animal Lab. of the present utility model can be applied to the measurement of the laboratory ammonia concentration under the disinfection situation of laboratory.

Description

The Portable environmental-monitoring device of Animal Lab.

Technical field

The present invention relates to laboratory environment monitoring device, more specifically to a kind of portable ring of Animal Lab. Border monitoring device.

Background technology

Experimental animal grows in artificial environment, breed and reception test processing, particular place and its surrounding space are real Animal environment is tested, in biomedical research, accurate, authentic experiments data is obtained, to avoid physiology of the environmental factor to animal Biochemical activity adversely affect, create a good experimental animal environment, every monitoring index animal living environment is played to Close important effect.Particularly important is exactly air purity and Particulate Pollution；Air purity index is directed to animal feeding Chamber interior air floating particulate matter (microorganism is more to be attached on particulate matter) and pernicious gas, main source are animal excrements Fermentation is decomposed.Wherein analyte includes ammonia, methyl mercaptan.Hydrogen sulfide, styrene, acetaldehyde etc..The rising of animal feeding environment temperature, Stocking density increases, ventilation condition is bad, excreta and the non-timely processing of bedding and padding can all cause ammonia density to steeply rise.And ammonia is made Can stimulating animal eye conjunctiva when being raised for pungent concentration.Nasal membrane and respiratory mucosa, which cause, sheds tears, coughs.Seriously Person produces acute pulmonary edema and causes animal dead.It is chronically at the animal upper respiratory tract under high strength ammonia environment and chronic inflammation occurs, Have a strong impact on animal health.Particulate matter index is different with particulate matter size, and particulate matter main source has two kinds of approach：It is outdoor empty Gas is filtered to be dealt with improperly and is brought directly to and animal skin, scurf, feed and bedding and padding etc. are often carried by air-flow or animal is kicked up Float in atmosphere.Similar to the mankind for animal, what it was had a great influence is the dust that diameter is less than less than 5 μm, this The flotation time is long in atmosphere for class dust, is drawn into through respiratory tract up to bronchiole and alveolar so as to cause breathing problem； In addition to itself produces harmful effect to animal, it impacts bigger grain thing as microbe carrier to animal health.

Laboratory Animal Facility is continuously run, and various environmental factors are had been among variation, it is also desirable to regular Monitoring and maintenance.Every environmental index requires very strict, various measurement apparatus and people in the residing experiment room of experimental animal simultaneously Member is required for the processing that carries out disinfection before entering, and traditional ammonia measuring apparatus can not be directly facing current sterilization demand.

According to the experiment of the mechanisms such as Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences, Beijing Experimental Amimal Research Centre Found in zooscopy：The species of China experimental animal is mostly mouse (rat, mouse, suslik, cavy, Immune deficient mice, disease mould Type mouse etc.), rabbit, chicken, dog, pig, pig (miniature pig etc.), tree shrew, monkey (macaque etc.) etc., such experimental animal all has fertility By force, the less feature of build.Laboratory Animal Facility is continuously run simultaneously, and various environmental factors are had been among variation, It is also required to regular monitoring and maintenance.To experimental animal environment and facility condition, there is the regulation of standardization in country, according to GB 14925-2010《Experimental animal environment and facility》Regulation, it is dense that detection project includes temperature, relative humidity, air purity, ammonia Degree, noise, illumination and ventilation volume etc..Therefore its experimental situation must be provided with various criterion for different animals, so as to maximum limit Degree reduces the experimental animal influence to experiment in itself.

The chemical method of ammonia has sodium hypochlorite-salicylic acid AAS, nessler reagent light splitting in existing measure air Photometry, indophenol blue reagent colorimetric method etc..The measurement of these chemical methodes has the advantages that the response time is short, measurement is sensitive, still Shortcoming is also quite obvious, measuring method and operating procedure it is numerous and diverse, it is necessary to by specialty labware, while can only also carry out Spot measurement, it is inadvisable for measurement detecting system.

Traditional electrochemical sensor is separated by one layer of electrolytic thin-membrane, and is connected by a low-impedance external circuit. On induction electrode surface oxidation or reduction reaction occur for the gas for diffusing into sensor, and an internal electricity is produced between two electrodes Stream, current value correspond to oxygen concentration.But when tested gas concentration continues to increase, induction electrode electromotive force will exceed and allow Scope, sensor output will become measurement concentration that is nonlinear, thus limiting gas.

The content of the invention

For the problem of ammonia measuring method is not applied for test in laboratory present in prior art, the purpose of the present invention It is to provide a kind of Portable environmental-monitoring device of Animal Lab..

To achieve the above object, the present invention adopts the following technical scheme that：

A kind of Portable environmental-monitoring device of Animal Lab., including main control module, ammonia gas sensor, TEMP Device, humidity sensor, sensor adapter.Ammonia gas sensor, temperature sensor and humidity sensor are respectively connecting to sensor Adapter, sensor adapter connection main control module, ammonia gas sensor include induction electrode, negative electrode and reference electrode, referred to Electrode connects outside mu balanced circuit to stablize the electromotive force of induction electrode.The signal of temperature sensor and humidity sensor is to strong Positive ammonia concentration data.

Further, main control module includes multiple A/D interfaces, ammonia gas sensor output analog signal, and passes through master control mould Block is configured to 8 or 12 digit word formats.

Further, in addition to display module, display module is connected to main control module, and main control module is by ammonia concentration data Transmit to display module.

Further, sensor adapter, main control module are arranged in a housing, ammonia gas sensor, temperature sensor and Humidity sensor is arranged at the unidirectional side of housing.

Further, the gas sensitive of ammonia gas sensor is tin ash.

Further, ammonia gas sensor includes 4 pins, and its signal is spread out of from 2,4 pins, and No. 4 leg signals are directly defeated Go out analog voltage signal, No. 2 leg signals are then amplified by amplifier and believed by exporting digital voltage after adjustable resistor partial pressure Number.

To achieve the above object, the present invention also adopts the following technical scheme that：

In the above-mentioned technical solutions, the Portable environmental-monitoring device and method of Animal Lab. of the invention can be applied The measurement of laboratory ammonia concentration under the disinfection situation of laboratory.

Brief description of the drawings

Fig. 1 is the structural representation of environment monitoring device of the present invention；

Fig. 2 is the circuit diagram of ammonia gas sensor and its peripheral circuit；

Fig. 3 is the flow chart of method of environmental monitoring of the present invention.

Embodiment

Technical scheme is further illustrated with reference to the accompanying drawings and examples.

Reference picture 1, the present invention disclose a kind of Portable environmental-monitoring device of Animal Lab., its main modular bag first Include main control module 1, ammonia gas sensor 3, temperature sensor 4, humidity sensor 5, sensor adapter 2, memory module 6, display Module 7, wireless communication module 8.Above-mentioned module is integrated in a housing, shell dimension 263*184*95mm, is prevented using plastics Water material, while there is high corrosion resistance, the signal shielding of metal shell is it also avoid, ensure that the normal transmission of signal. Air outlet valve uses plastic cable water joint, and classification of waterproof can reach IP67.

As shown in figure 1, ammonia gas sensor 3, temperature sensor 4 and humidity sensor 5 are respectively connecting to sensor adapter 2, sensor adapter 2 connects main control module 1, and ammonia gas sensor 3 includes induction electrode, negative electrode and reference electrode, with reference to electricity Pole connects outside mu balanced circuit to stablize the electromotive force of induction electrode.The signal of temperature sensor 4 and humidity sensor 5 is to strong Positive ammonia concentration data.Display module 7 is LCD module, and it is connected to main control module 1, and main control module 1 passes ammonia concentration data Transport to display module 7.Wireless communication module 8 remotely obtains data for device, because this portable unit portability is strong, flowing Property it is big, then transmission range may be very big, and simultaneous transmission data are smaller, therefore have selected GPRS transmission mode, while in view of different Different wireless communication modules 8 can be added with place, such as WIFI module is transmitted by router, bluetooth module etc. Deng.

As a portable set, sensor adapter 2, main control module 1 may be contained within a housing, ammonia gas sensor 3rd, temperature sensor 4 and humidity sensor 5 are arranged at the unidirectional side of housing.Because the device of the present invention is directed to animal Laboratory measures, and its object is the mixed gas with ammonia of the excreta release of animal, and such mixed gas Amount less, skewness, there is directive property and regionality.Therefore, this have the advantage that the He of temperature sensor 4 by the present invention Humidity sensor 5 and ammonia gas sensor 3 are substantial access to, in terms of the number of degrees of correction ammonia gas sensor 3 advantageously.

In the present invention, main control module uses high performance ARMCortex- using STM32F103 microcontrollers as core The RISC core of M332 positions, working frequency 72MHz, internal high-speed memories (the up to flash memory and 20K bytes of 128K bytes SRAM), abundant enhancing I/O ports and the peripheral hardware for being connected to two APB buses.Device include 2 12 ADC, 3 it is general 16 bit timing devices and a PWM timer, also comprising standard and advanced communication interface：Up to 2 I2C and SPI, 3 USART, USB and CAN.The efficient conversion for the signal of ammonia gas sensor 3 is ensure that, while is also later stage module Addition and transplanting provide possibility.STM32 DAC module (numeral/analog conversion module) is that 12 bit digitals input, voltage The DAC of output type.The output of the module of ammonia gas sensor 3 is exactly voltage analog signal, and it is connected to any A/D of STM32F103 and drawn After pin, 8 or 12 bit patterns are configured, while input reference voltage VREF+ just obtains more accurate transformation result, that is, is converted into phase The data signal answered.Its voltage, ammonia concentration curved line relation is recycled to obtain after the module carry-out bit voltage of ammonia gas sensor 3 is obtained Function is obtained, corresponding ammonia concentration data are can obtain by conversion processing, so as to complete ammonia concentration measurement work.

In addition to STM32F103 main control modules, remaining module is all added on main control module 1, is controlled by main control module 1 The acquisition of ammonia gas sensor 3 and Temperature Humidity Sensor data-signal processed；Changed into afterwards after program is handled ammonia concentration and Data of the Temperature and Humidity module, and make its display on the lcd screen, while carry out the storage of data.

Reference picture 2, the ammonia gas sensor 3 in Fig. 1 employ the metering system of three-electrode electro Chemical.Three electrode ammonias sense Device is on the basis of two electrode sensors, by accessing outside mu balanced circuit, and adds reference electrode to stablize induction electrode Electromotive force.In this way, persistently polarized even if negative electrode also will not produce any influence to induction electrode, so three electrode ammonias sense Utensil has wider array of measurement range.

As shown in Fig. 2 the energization such as power supply instruction lights after ammonia gas sensor (MQ sensors) energization, its gas sensitive is clear The relatively low tin ash of electrical conductivity in clean air, when sensor local environment has ammonia, sensor conductance rate is with air Middle concentration increases and increased, and signal is spread out of from MQ sensors 2,4 pins, and No. 4 leg signals directly export analog voltage signal (AOUT), No. 2 leg signals are then amplified and by being exported after adjustable resistor partial pressure by amplifier LM393AD, i.e. digital voltage Signal (DOUT).Wherein adjustable resistor acts on the regulation and zeroing for sensor assembly, is adjusted when in without ammonia environment Section knob makes alarm lamp be in bright with not bright limb；When ammonia environment measurement be present, due to voltage rise, then indicator lamp is bright Rise.

In the present invention, electrochemical ammonia sensor ME3-NH3 works according to the principle of electrochemistry, is existed using under test gas Electrochemical oxidation process in electrolytic cell on working electrode, it is by electronic circuit that the working electrode of electrolytic cell and reference electrode is permanent An appropriate current potential is scheduled on, the electrochemical oxidation of under test gas can occur under the current potential, because oxygen is being aoxidized and reduced Caused faradic currents very little, can be ignored during reaction, then electric current caused by under test gas electrochemical reaction It is directly proportional to its concentration and follow Faraday's law.So, it is assured that the dense of under test gas by determining the size of electric current Degree.

Reference picture 3, using the detection method of apparatus of the present invention, it includes following key step：

S1：System first carries out initialization detection after starting, and determines just to start to survey after sensor assembly and other modules are normal Amount prepares.

S2：Start electronic valve and be evacuated.Specifically, open magnetic valve and open air exhauster pumping, treat inside device Gas pumping closes magnetic valve when complete, and device is internally formed class vacuum state and (is actually unable to reach vacuum, but will portion big absolutely Gas is divided to extract out).

S3：Judge whether to add wireless network moduleIf so, continue subsequent step, if it is not, returning to S2.

S4：Treat to carry out disinfection processing (spray or immersing type disinfection) according to Laboratory Request after the completion of gas pumping.

S5：Experiment room to be measured is put into after sterilization completely, that is, is put into laboratory under test gas environment and is opened magnetic valve, due to Ambient pressure is more than air pressure in device, then can be full of inside device by gas.

S6：Now, judge whether to trigger ammonia gas sensor measurement eventIf so, then enter S8, if it is not, then entering S7.

S7：Etc. ammonia gas sensor measurement event to be triggered, and return to S6.

S8：Ammonia concentration is changed into through measuring circuit data measured and by function program, is set if measurement gas exceedes Determine zoopery room ammonia standard and then carry out sound and light alarm

S9：LCD shows ammonia concentration data, and data are then constantly sent to client by wireless sensing device (i.e. GPRS module) Hold, constantly circulation measurement is until closing measurement after data measured.

After measurement terminates, device internal gas is extracted out, to avoid influence of the irritant gas to internal sensor, dropped simultaneously Influence of the low previous measurement gas to measurement next time.So far measurement terminates.

Those of ordinary skill in the art it should be appreciated that the embodiment of the above be intended merely to explanation the present invention, And be not used as limitation of the invention, as long as in the spirit of the present invention, the change to embodiment described above Change, modification will all fall in the range of claims of the present invention.

Claims (6)

1. A kind of 1. Portable environmental-monitoring device of Animal Lab., it is characterised in that including：

   Main control module, ammonia gas sensor, temperature sensor, humidity sensor, sensor adapter；

   The ammonia gas sensor, temperature sensor and humidity sensor are respectively connecting to the sensor adapter, the sensing Device adapter connects the main control module；

   The ammonia gas sensor includes induction electrode, negative electrode and reference electrode, the outside mu balanced circuit of reference electrode connection To stablize the electromotive force of induction electrode；

   The signal of the temperature sensor and humidity sensor is correcting ammonia concentration data.
2. 2. the Portable environmental-monitoring device of Animal Lab. as claimed in claim 1, it is characterised in that the main control module Including multiple A/D interfaces, ammonia gas sensor output analog signal, and 8 or 12 bit digitals are configured to by main control module Form.
3. 3. the Portable environmental-monitoring device of Animal Lab. as claimed in claim 1, it is characterised in that also include display mould Block, the display module are connected to the main control module, and the main control module is by ammonia concentration data transfer to the display mould Block.
4. 4. the Portable environmental-monitoring device of Animal Lab. as claimed in claim 1, it is characterised in that the sensor is fitted Orchestration, main control module are arranged in a housing, and the ammonia gas sensor, temperature sensor and humidity sensor are arranged at housing Unidirectional side.
5. 5. the Portable environmental-monitoring device of Animal Lab. as claimed in claim 1, it is characterised in that the ammonia sensing The gas sensitive of device is tin ash.
6. 6. the Portable environmental-monitoring device of Animal Lab. as claimed in claim 1, it is characterised in that the ammonia sensing Device includes 4 pins, and its signal is spread out of from 2,4 pins, and No. 4 leg signals directly export analog voltage signal, No. 2 leg signals Then amplified and by exporting digital voltage signal after adjustable resistor partial pressure by amplifier.