CN108403493A - A kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and the preparation method and application thereof - Google Patents
A kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and the preparation method and application thereof Download PDFInfo
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- CN108403493A CN108403493A CN201810289710.9A CN201810289710A CN108403493A CN 108403493 A CN108403493 A CN 108403493A CN 201810289710 A CN201810289710 A CN 201810289710A CN 108403493 A CN108403493 A CN 108403493A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/02—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings containing insect repellants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The present invention provides a kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and the preparation method and application thereof.The method is as follows：Raw material Asian puccoon, honeysuckle flowers and camellia seed are pre-processed to obtain mixed plant piece；Mixed plant piece obtained is placed in 20~45 DEG C of soak and is impregnated 15~30 minutes, soak includes 5~10 parts of the first enzyme preparation, 1~5 part of alcohols material and 40~100 parts of water；Plant piece by soak and after impregnating is ultrasonically treated 1~2 hour under preset temperature, isolated supernatant after static layering；Supernatant heating is cooled to after room temperature and compounds itself and the second enzyme preparation to obtain mixed liquor using ultrasonic wave；Mixed liquor removes impurity, eluent, that is, mosquito-expelling and antipruritic plant extraction liquid of acquisition using the chromatography column pure water elution for being filled with resin extender.Mosquito-expelling and antipruritic plant extraction liquid provided by the invention is mildly non-stimulated, and mosquito-expelling and antipruritic effect is good, and has natural anticorrosion performance.
Description
【Technical field】
The present invention relates to Enzymatic Extraction active material technical field more particularly to a kind of biological enzyme processing and corrosion-resistant drive
Antipruritic plant extraction liquid of mosquito and the preparation method and application thereof.
【Background technology】
Asian puccoon is clinical conventional Chinese medicine, derives from comfrey lithospermum euchromum Royle Arnebia euchroma (Royle)
Johnst, Asian puccoon Lithospermum erythrorhizon Sieb.et Zcc. or arnebia guttata Bunge Arnebia guttata
The dry root of Bunge is used with the effect of cool blood, promoting blood circulation, removing toxic substances promoting eruption.The active ingredient of Asian puccoon be the naphthoquinones class such as alkannin at
Point, pharmacological research shows that alkannin has the pharmacology promoting blood circulation such as antibacterial, anti-inflammatory, antiviral.
Honeysuckle flowers (honeysuckle) are the dry flower of Caprifoliaceae perennial evergreen twining plant or the flower just opened.Its bitter,
It is cold in nature, there is clearing heat and detoxicating, wind-heat dissipating, hepatic cholagogic and other effects, be traditional medicinal and edible plant.With advantageous to human body
Active components of plants, such as chlorogenic acid, isochlorogenic acid, cyanidenon flavone compound.Flavone compound has higher
Bioactivity enhance immunity of organisms, anti-oxidant and Green Tea Extract, anti-cancer and cancer-preventing, and nontoxic nothing such as antibacterial, antiviral
Evil.The extract of honeysuckle can heat-clearing anti-acne, promote cell metabolism, provide nutrition for skin, help skin discharge toxin, enable flesh
The smooth profit of skin is white.
Camellia seed camellia oil is full of nutrition, fatty acids, camellianin, tea polyphenols, saponin, tannin and rich in antioxidant and
Squalene with anti-inflammatory effect, squalene and Flavonoid substances play the role of anticancer splendid.Camellia oil is also rich in dimension life
Plain E and calcium, iron, zinc etc. are micro-, and the Zn-ef ficiency of " flowers of life " is described as by physician and nutritionist.
The extraction species of current antipruritic mosquito proof are few, and the overwhelming majority is single plant extract, and are mostly to have
Solvent method is extracted, and there are organic solvent allergy equivalent risks in extract, cause the antipruritic mosquito proof effect of extract bad.
【Invention content】
The purpose of the present invention is to provide it is a kind of with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid method,
To overcome extract safety caused by prior art organic solvent extraction and both effectiveness not high technological deficiency.
In order to achieve the above objectives, the present invention provides a kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid
Preparation method, the preparation method include the following steps：
(1) 10~80 portions of Asian puccoons of raw material, 10~80 parts of honeysuckle flowers and 10~80 parts of camellia seeds are pre-processed to obtain length small
In the mixed plant piece of preset value；
(2) mixed plant piece made from step (1) is placed in 20~45 DEG C of soak and is impregnated 15~30 minutes, it is described
Soak includes 5~10 parts of the first enzyme preparation, 1~5 part of alcohols material and 40~100 parts of water, wherein first enzyme preparation
For the mixture of one or more of cellulase, pectase, zytase and hemicellulase, the alcohols material
For the mixture of one or more of 1,2- hexylene glycols, butanediol, propylene glycol, pungent glycol；
(3) the mixed plant piece by soak and after impregnating is transferred in processor for ultrasonic wave, ultrasonic at 20~45 DEG C
Processing 1~2 hour, isolated supernatant after static layering；
(4) supernatant is heated 5~15 minutes at 85~100 DEG C, after being cooled to room temperature, adds 5~10
Second enzyme preparation of part, and be ultrasonically treated 1~2 hour at 20~45 DEG C and obtain mixed liquor, wherein the second enzyme preparation packet
Include glucose oxidase and/or lysozyme；
(5) mixed liquor removes impurity, acquisition is washed using the chromatography column pure water elution for being filled with resin extender
De- liquid, that is, mosquito-expelling and antipruritic plant extraction liquid.
In one preferred embodiment of preparation method of the mosquito-expelling and antipruritic plant extraction liquid provided by the invention, the purple
The weight ratio of careless, the described honeysuckle flowers and the camellia seed is (0.25~2)：(0.5~8)：1.
In the preparation method with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid provided by the invention
In one preferred embodiment, the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is 2：1：1.
In the preparation method with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid provided by the invention
In one preferred embodiment, second enzyme preparation further includes Co-Q10 or protease.
The present invention also provides a kind of with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid, the mosquito-expelling and antipruritic plant
Object extracting solution is prepared into using the preparation method described above with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid
It arrives.
The present invention also provides a kind of mosquito-expelling and antipruritic plant extraction liquids described above in preparing cosmetics and daily chemical product
Application.
Be in terms of 100% by its total weight the present invention also provides a kind of mosquito-expelling and antipruritic cream comprising 5.0~15% it is above
The mosquito-expelling and antipruritic plant extraction liquid that the preparation method is prepared.
The present invention also provides a kind of mosquito-expelling and antipruritic liquid detergents, are in terms of 100% by its total weight comprising 0.1~15%
The mosquito-expelling and antipruritic plant extraction liquid that preparation method described above is prepared.
The present invention also provides a kind of mosquito-expelling and antipruritic baby Jinsui River, are in terms of 100% by its total weight comprising 3~18%
The mosquito-expelling and antipruritic plant extraction liquid that preparation method described above is prepared.
Compared with the relevant technologies, it is provided by the invention with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and its
Preparation method has the advantages that with application：
One, for mosquito-expelling and antipruritic plant extraction liquid provided by the invention using Asian puccoon, honeysuckle flowers and camellia seed as raw material, utilization is compound
It is thin to crack plant under the first enzyme preparation and the collective effect of alcohols material comprehensively for the peculiar technology extraction active material of biological enzyme
The closed structure of cell wall and cytoplasm, the active material in high efficiency extraction raw material, and avoid using virose organic molten
Agent improves the safety of mosquito-expelling and antipruritic plant extraction liquid.
Two, the active material for effectively compounding the second enzyme preparation using ultrasonic wave and being extracted from raw material, makes the mosquito repellent of preparation
Antipruritic plant extraction liquid itself has antiseptic property, addition preservative is no longer needed to, on the one hand in cosmetics and personal care and toiletry
Using when can be to avoid negative effects such as the stimulations that bring of addition preservative, on the other hand can also extend product itself uses the longevity
Life.
Three, mosquito-expelling and antipruritic plant extraction liquid provided by the invention is soluble liquid, overcomes powdered moisturizing extract
The limitation of compliant applications in various product system, and mosquito-expelling and antipruritic plant extraction liquid provided by the invention is mildly stingless
Swash, antiallergy ability is strong.
【Specific implementation mode】
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only it is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
All other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of preparation method with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid, the side
Method includes the following steps：
Step 1: 10~80 portions of Asian puccoons of raw material, 10~80 parts of honeysuckle flowers and 10~80 parts of camellia seeds are pre-processed and are grown
Mixed plant piece of the degree less than preset value；
Specifically, the Asian puccoon is selected from lithospermum euchromum Royle Arnebia euchroma (Royle) Johnst, Asian puccoon
One kind of Lithospermum erythrorhizon Sieb.et Zcc. or arnebia guttata Bunge Arnebia guttata Bunge
Or two or more mixture.Preferably, the Asian puccoon is lithospermum euchromum Royle Arnebia euchroma (Royle) Johnst.Institute
State one of honeysuckle flowers in Guangdong region honeysuckle flowers, Hubei Regional honeysuckle flowers, Hainan region honeysuckle flowers, Jiangsu region honeysuckle flowers
Kind or two or more mixtures.Preferably, the honeysuckle flowers are Guangdong region honeysuckle flowers.The camellia seed is selected from Guangxi District
The mixing of one or more of domain camellia seed, Jiangxi region camellia seed, Zhejiang region camellia seed, Jiangsu region camellia seed
Object.Preferably, the camellia seed is Guangxi region camellia seed.
Formula is weighed into Asian puccoon, honeysuckle flowers and camellia seed, and is cleaned up, little block is cut into, then reuses juice extractor
Broken, the length of broken each raw material is less than 8mm, i.e., is less than 8mm by the length of the broken mixed plant piece of juice extractor.
It should be noted that Asian puccoon, honeysuckle flowers and camellia seed can be remixed with crusher machine of squeezing the juice respectively, or will be purple before broken
Grass, honeysuckle flowers and camellia seed mixing, are then crushed together again.Degree of crushing is caused to raw material using the juice extractor of different model
Different, 8mm is preferred value.
Preferably, the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is (0.25~2)：(0.5~8)：
1。
Preferably, the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is 1:1:1、1:2:1、1:4:1、
1:6:1、1:8:1、2:1:1、4:1:2、6:1:2 and 8:1:2.
It is further preferable that the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is 2：1：1.
It is impregnated 15~30 minutes Step 2: mixed plant piece made from step 1 is placed in 20~45 DEG C of soak,
The soak includes 5~10 parts of the first enzyme preparation, 1~5 part of alcohols material and 40~100 parts of water, wherein first enzyme
Preparation is the mixture of one or more of cellulase, pectase, zytase and hemicellulase, the alcohols
Substance is the mixture of one or more of 1,2- hexylene glycols, butanediol, propylene glycol, pungent glycol.
The present invention extracts active material using the peculiar technology of compound biological enzyme, in the common of the first enzyme preparation and alcohols material
Under effect, the closed structure of plant cell wall and cytoplasm, the work of high efficiency extraction Asian puccoon, honeysuckle flowers and camellia seed are cracked comprehensively
Property substance, and avoid using virose organic solvent, improve the safety of mosquito-expelling and antipruritic plant extraction liquid.
Preferably, first enzyme preparation is the mixture of the cellulase, the pectase and the zytase,
And the weight ratio group of the zytase, the cellulase and the pectase sum becomes 1：(2~8)：(1~6).
Wherein, cellulase can make the mass degradations such as cellulose, pectin in cell wall or cytoplasm with pectase,
The compact texture for destroying cell wall causes cell wall and cytoplasm structure to generate the variations such as loose part, expansion, collapse, subtracts
The resistance to mass tranfer that the mass transfers such as cellule wall, cytoplasm barrier spreads active ingredient from intracellular to Extraction medium, from mass transfer angle
Degree promotes effective component extraction rate to improve.
Xylan is the important component of plant hemicellulose, and xylan is complicated, the degradable ginseng for needing a variety of enzymes
With, and zytase is to participate in most important one kind in degradation enzyme system.Xylan refer to can by xylan degrading at oligosaccharide and
The general name of one group of enzyme of xylose, zytase can be crushed plant cell wall, improve nutrient xylan degrading be the degree of polymerization compared with
Low small molecule segment destroys the structure of cell wall, and the nutriment that cell wall fetters is released.
Hemicellulase is that a kind of polysaccharide (except cellulose and pectin substance) that can make composition plant cell membrane hydrolyzes
Enzyme.
Wherein, 1,2- hexylene glycols, butanediol, propylene glycol and pungent glycol can dissolve certain plant ingredient, simultaneously
Safety is good, has respective good characteristic.Such as 1,2- hexylene glycols, can efficiently permanent moisturizing, also have the work(of antisepsis and sterilization
Effect；Butanediol sense of touch is smooth, and property is stablized, and more soft skin sense can be obtained；The viscosity and good hygroscopicity of propylene glycol, it is nontoxic；
Pungent glycol has certain moisturizing and antibacterial activity.
Preferably, the alcohols material is propylene glycol.
Step 3: the mixed plant piece by soak and after impregnating is transferred in processor for ultrasonic wave, at 20~45 DEG C
It is ultrasonically treated 1~2 hour, isolated supernatant after static layering；Wherein, separation method can be centrifugation or filtering.
Under normal circumstances, in order to reduce the preparation process time, the temperature of supersound process is identical with the temperature of immersion.
Step 4: the supernatant is heated 5~15 minutes at 85~100 DEG C, after being cooled to room temperature, 5 are added
~10 parts of the second enzyme preparation, and be ultrasonically treated 1~2 hour at 20~45 DEG C and obtain mixed liquor, wherein the second enzyme system
Agent includes glucose oxidase and/or lysozyme；
Preferably, second enzyme preparation further includes Co-Q10 or protease.
Second enzyme preparation is both effectiveness enzyme preparation, and glucose oxidase and lysozyme can inhibit bacterial plaque to be formed, can effectively go
Degerming planting group makes the mosquito-expelling and antipruritic plant extraction liquid itself of extraction have antiseptic property, extends the service life of product, and keep away
Exempting from other preservatives are added makes generation allergic phenomena.The effect of it is in application can be improved in Co-Q10 and protease.
Specifically：
Glucose oxidase effectively fast decoupled glucan, dissolving bacterial plaque can inhibit bacterial plaque to be formed, can effectively remove bacterium colony
Population.
Lysozyme is a kind of alkaline enzyme that can be hydrolyzed and stick polysaccharide in pathogenic bacteria.Mainly by destroying the N- acetyl in cell wall
β-Isosorbide-5-Nitrae glycosidic bond between muramic acid and n-acetylglucosamine makes the insoluble glutinous polysaccharide of cell wall resolve into soluble glycopeptide,
Cell wall rupture content is caused to escape and make bacterolysis.Lysozyme can also be bound directly with negatively charged virus protein,
Double salt is formed with DNA, RNA, apoprotein, makes virally inactivated, therefore, which has the effects that antibacterial, anti-inflammatory, antiviral.
Lysozyme is safe as preservative.Lysozyme is a kind of native protein, and property is very stable.
Co-Q10 is a kind of fat-soluble antioxidant, and the nutrition of energy human activin cell and cellular energy has and improves people
Body immunity enhances the functions such as anti-oxidant, anti-aging and enhancing human activity.Can go deep into cell simultaneously, strengthen cell newly at
On the other hand metabolic function, consolidation binding ability between Polygonum Multiflorum Thunb Extract form the reticular structure of elasticity in epidermis, repair really because
Wrinkle caused by fluid loss characteristics, and reach real moisture-keeping efficacy in turn.
Step 5: the mixed liquor is removed impurity, is obtained using the chromatography column pure water elution for being filled with resin extender
Eluent, that is, mosquito-expelling and antipruritic plant extraction liquid.
The present invention also provides what the preparation method using mosquito-expelling and antipruritic plant extraction liquid described above was prepared to use life
Object enzyme is processed and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid, which can prepare cosmetics and daily use chemicals
It is applied in articles for use.
It is specific in itching-relieving cream, liquid detergent and baby Jinsui River that the invention further particularly discloses mosquito-expelling and antipruritic plant extraction liquids
Using.The technical solution of specific implementation is as follows：
It is in terms of 100% by its total weight comprising 5~15% system described above using a kind of 1, mosquito-expelling and antipruritic cream
The mosquito-expelling and antipruritic plant extraction liquid that Preparation Method is prepared.
Preferably, the mosquito-expelling and antipruritic cream includes following component by weight percentage：Glycerine 4.5~6.5%, fourth two
Alcohol 2.0%~3.0%, allantoin 0.15%~0.20%, gluconic acid sodium salt 0.008%~0.01%, caprylic/capric triglyceride
2.0%~3.5%, Parleam 2.0%~3.0%, isononyl isononanoate 0.5%~1.5%, cetostearyl alcohol
0.1%~0.8%, polyacrylate cross-linked polymer -6 0.2%~0.8%, mosquito-expelling and antipruritic plant extraction liquid 5.0%~
15%, citric acid 0.01%~0.05%, surplus are deionized water.
It is highly preferred that the mosquito-expelling and antipruritic cream includes following component by weight percentage：Glycerine 4.5%, butanediol
2.3%, allantoin 0.2%, gluconic acid sodium salt 0.01%, caprylic/capric triglyceride 2.5%, Parleam 2.5%, different
The different nonyl ester 1.5% of n-nonanoic acid, cetostearyl alcohol 0.5%, polyacrylate cross-linked polymer -6 0.8%, mosquito-expelling and antipruritic plant extract
Liquid 5.0%, citric acid 0.01%, deionized water 80.18%.
It is in terms of 100% by its total weight comprising 0.1~15% institute above using a kind of 2, mosquito-expelling and antipruritic liquid detergent
The mosquito-expelling and antipruritic plant extraction liquid that the preparation method stated is prepared.
Preferably, the mosquito-expelling and antipruritic liquid detergent includes following component by weight percentage：Fatty acid distribution of coconut oil amide
Base propyl betaine 4.5~6.5%, sodium laureth sulfate 2.0%~5.0%, ethoxylated alkyl sulfate
6.5%~8.5%, mosquito-expelling and antipruritic plant extraction liquid 0.1%~15%, citric acid 0.1%~0.5%, surplus are deionized water.
It is highly preferred that the mosquito-expelling and antipruritic liquid detergent includes following component by weight percentage：Fatty acid distribution of coconut oil acyl
Aminocarbonyl propyl glycine betaine 5.0%, sodium laureth sulfate 2.0%, ethoxylated alkyl sulfate 8.5%, mosquito repellent
Antipruritic plant extraction liquid 5.0%, citric acid 0.1%, deionized water 79.4%.
It is in terms of 100% by its total weight comprising 3~18% institute above using a kind of 3, mosquito-expelling and antipruritic baby Jinsui River
The mosquito-expelling and antipruritic plant extraction liquid that the preparation method stated is prepared.
Preferably, mosquito-expelling and antipruritic baby Jinsui River includes following component by weight percentage：Glycerine 4.5%~
6.5%, mosquito-expelling and antipruritic plant extraction liquid 3.0%~18%, butanediol 0.8%~2%, borneol 0.01%~0.08%, lemon
Acid 0.01%~0.05%, surplus are deionized water.
It is highly preferred that mosquito-expelling and antipruritic baby Jinsui River includes following component by weight percentage：Glycerine 5.0%,
Mosquito-expelling and antipruritic plant extraction liquid 16%, butanediol 2%, borneol 0.01%, citric acid 0.05%, deionized water 76.94%.
One, mosquito-expelling and antipruritic plant extraction liquid (embodiment 1-9) is prepared
Embodiment 1
20 portions of Asian puccoons, 10 parts of honeysuckle flowers and 10 parts of camellia seeds are cleaned up, after being cut into small pieces, mixing uses juice extractor
It is broken；Then 50 parts of water, 9.5 part of first enzyme preparation (3.5 parts of cellulase, 3.5 parts of pectase, 2.5 parts of zytase) is added
With 1 part of alcohols material (1 part of propylene glycol), it is placed in 25 DEG C of thermostatic water-baths and impregnates 30min；Ultrasonic wave is transferred to after immersion
In processor, to continue ultrasound 1h at a temperature of 25 DEG C；It is then allowed to stand layering, centrifuges or be obtained by filtration supernatant；Again by supernatant
Liquid heats 10min at 90 DEG C, and after supernatant is cooled to room temperature, it is (2 parts of glucose oxidase, molten that 5.5 part of second enzyme preparation is added
3.5 parts of bacterium enzyme), continuation ultrasound 1h obtains mixed liquor at 25 DEG C；Finally by the mixed liquor by being filled with resin extender
Chromatography column, is washed with water, and to remove impurity, collects eluent to get to mosquito-expelling and antipruritic plant extraction liquid.
In embodiment 1, the weight ratio of centella, chamomile and Radix Glycyrrhizae is 2：1：1, embodiment 2~9 remove by centella,
Other than the weight ratio of chamomile and Radix Glycyrrhizae changes as shown in table 1, under the conditions of other are all same with embodiment 1 into
It goes and mosquito-expelling and antipruritic plant extraction liquid is prepared, the weight ratio of each raw material is as follows in embodiment 2~9：
Table 1
Two, the safety for the mosquito-expelling and antipruritic plant extraction liquid that embodiment provides in order to further illustrate the present invention and effect
Property, the advantageous effect of the mosquito-expelling and antipruritic plant extraction liquid of embodiment offer is be provided below by way of test example：
Experiment one：Mosquito-proof sting experiment
Laboratory sample：The mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 1~9 provides
Experimental method：It will be packed into the fresh pig blood of 50mL in clean beaker, the distilled water of 50mL is then added as empty
White control, number 1；The fresh pig blood of 50mL will be packed into clean beaker, the mosquito repellent being then added in the embodiment 1 of 50mL stops
Itch plant extraction liquid, number 2；It will be packed into the fresh pig blood of 50mL in clean beaker, is then added in the embodiment 2 of 50mL
Mosquito-expelling and antipruritic plant extraction liquid, and so on, number 3；Number 4 corresponds to the mosquito-expelling and antipruritic plant that the embodiment of the present invention 3 provides and carries
Take liquid；The mosquito-expelling and antipruritic plant extraction liquid of 5 corresponding embodiment 4 of number；The mosquito-expelling and antipruritic plant extract of 6 corresponding embodiment 5 of number
Liquid；The mosquito-expelling and antipruritic plant extraction liquid of 7 corresponding embodiment 6 of number；The mosquito-expelling and antipruritic plant extraction liquid of 8 corresponding embodiment 7 of number；
The mosquito-expelling and antipruritic plant extraction liquid of 9 corresponding embodiment 8 of number；The mosquito-expelling and antipruritic plant extraction liquid of 10 corresponding embodiment 9 of number.
1~No. 10 beaker of number is covered into big cloche with holes successively, pours into 10 mosquitoes successively from glass orifice,
Glass orifice is sealed, the active state of mosquito in beaker in 0~5min is observed.
Mosquito repellent rate=(mosquito populations/mosquito total quantity in mosquito total quantity-blood solution) × 100%.
Table 2
By the experimental result of table 2 it is found that the antipruritic mosquito proof compound extracted solution repelling effect of the present invention is good, mosquito repellent rate is high
It, can effective mosquito-proof sting up to 100%.
Experiment two：Antipruritic effect is tested
1, test specimen：The mosquito-expelling and antipruritic plant extraction liquid that Examples 1 to 9 provided by the invention provides
2, effect of itching is gone：From a certain region select 225 volunteers, the age of volunteer between 20-45 Sui, body
Health, in the recent period non-drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects.Volunteer is divided into 9 at random
Group, every group of 25 people.After bite by mosquitos, antipruritic mosquito proof compound extracted solution is applied to skin and bites place, observes antipruritic effect.
By volunteer by after bite by mosquitos, using Contrast on effect before and after antipruritic mosquito proof compound extracted solution, using questionnaire
Form collects data, and antipruritic effect is subject to volunteer's feedback effects data：
Wherein antipruritic rate=100% × number (antipruritic to evaluate)/total number of persons
Table 3
Volunteer does not occur adverse reaction (such as facial redness, allergy etc.), stopping in the present invention during experience
Mosquito proof of itching extracting solution is fabulous for antipruritic effect caused by after bite by mosquitos, and antipruritic rate is up to 100%.
Experiment three：Preservative efficacy testing
1, test specimen：The mosquito-expelling and antipruritic plant extraction liquid sample that the embodiment of the present invention 1 provides；Experimental bacteria is Escherichia coli
ATCC 25922, staphylococcus aureus ATCC 6538, pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC
10231。
2, test method：Microbiology challenge test method.
3, result evaluation：Using《European Pharmacopoeia》Result evaluation standard this test result is evaluated, have
Body refers to the following table 3.
Table 4
From the test result of upper table 4 it is found that 4 kinds of experimental bacterias (1 week) total plate count when the 7th day declines 100%, until the
It is tested by challenge without growth, judgement at 28 days, inhibitor effectiveness is good, i.e., the mosquito-expelling and antipruritic that the embodiment of the present invention 1 provides is planted
Object extracting solution is good using the anti-corrosion effect of the protective system of lysozyme, glucose oxidase and polyalcohol compounding structure.
Experiment four：Ocular irritation/erosion test
1, test specimen：The mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 1~9 provides.
2, test method：SN/T 2329-2009 cosmetics eye irritation/corrosive chick chorioallantoic membrane experiment.
3, evaluation of test result standard：Stimulation standards of grading are divided into following level：IS ＜ 1 are nonirritant；1≤IS ＜ 5
For subexcite；5≤IS ＜ 9 are moderate irritation；IS >=10 are strong and stimulating/corrosivity.
4, test result：Through overtesting, the stimulation scoring of mosquito-expelling and antipruritic plant extraction liquid of the invention is IS ＜ 1, i.e.,
Mosquito-expelling and antipruritic plant extraction liquid provided in an embodiment of the present invention is mildly nonirritant to eye.
Experiment five：Skin irritation test
(1) skin closure type patch test
1, test specimen：The mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 1~9 provides.
2, test method：From 20 volunteers of a certain regional choice, the age of volunteer, body was strong between 20~45 years old
Health, in the recent period non-drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects.Volunteer is divided into 10 groups at random,
Every group of two people.Wherein, group 1 is blank control group, and the small interior of spot examination device does not place any substance, spot is tried the low sensitization glue of device
Band is pasted on the back of volunteer or forearm bends side, is gently pressed with palm and is allowed to equably stick on the skin；Group 2~groups 10 with
The processing method of group 1 is identical, unlike：It will be put respectively added with 0.025mL mosquito-expelling and antipruritic plant extraction liquids provided by the invention
Enter the small interior of spot examination device；I.e. group 2 it is corresponding be embodiment 1 provide mosquito-expelling and antipruritic plant extraction liquid, group 3 it is corresponding be the present invention
The mosquito-expelling and antipruritic plant extraction liquid that embodiment 2 provides, corresponding group 4 is that the mosquito-expelling and antipruritic plant that the embodiment of the present invention 3 provides carries
Take liquid ..., it is mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 9 provides that group 10 is corresponding.Each group experiment is held
After 24 hours, 48 hours continuous, 30min after removal spot examination device, after impression disappearance, observation sticks the response situation and note of place's skin
Record.
Wherein, skin closure type patch test dermoreaction grade scale see the table below 5.
Table 5
During entire experiment, the subject's of group 1~groups 10 is reacted negative by gesture area skin.Thus
As can be seen that mosquito-expelling and antipruritic plant extraction liquid provided in an embodiment of the present invention will not cause the allergy and stimulate the reaction of skin, peace
Good perfection.
(2) repeatability is opening smears experiment
1, test specimen：The mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 1~9 provides.
2, from a certain region select 20 volunteers, the age of volunteer between 20-45 Sui, health, in the recent period
Non- drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects.Volunteer is divided into 10 groups, every group two at random
It is personal.Wherein, group 1 is blank control group, is bent using forearm as recipient site, 3 × 3cm2 of area, and recipient site keeps drying,
It avoids contact with before tested and during by gesture other external preparations, with ultra-pure water 0.05 ± 0.005g/ times, equably smears tested portion
Position；Group 2~groups 10 are respectively test group, unlike group 1：The mosquito-expelling and antipruritic plant provided using the embodiment of the present invention 1~9
Extracting solution 0.05 equably smears recipient site ± 0.005g/ times；Wherein, i.e., it is that the mosquito repellent that embodiment 1 provides stops that group 2 is corresponding
Itch plant extraction liquid, it is mosquito-expelling and antipruritic plant extraction liquid that the embodiment of the present invention 2 provides that group 3 is corresponding, and corresponding group 4 is this hair
The mosquito-expelling and antipruritic plant extraction liquid ... that bright embodiment 3 provides and so on, corresponding group 10 is that the embodiment of the present invention 9 provides
Mosquito-expelling and antipruritic plant extraction liquid.Each group carries out 2 times and equably smears recipient site daily, and long run test completes experiment after 7 days, often
The dermoreaction situation of the recipient site of its observation subject simultaneously records.During experiment, if there is in such as the following table 6
When 3 points or more of dermoreaction situation, it can stop continuing to test and record result.
Wherein, table 6 is that opening smear of skin repeatability tests dermoreaction judgment criteria table.
Table 6
During entire experiment, the dermoreaction by gesture position of the subject of group 1~groups 10 is negative.By
This, which can be seen that mosquito-expelling and antipruritic plant extraction liquid provided in an embodiment of the present invention, will not cause the allergy and stimulate the reaction of skin,
Safety is good.
Three, Application Example 10
The application of mosquito-expelling and antipruritic plant extraction liquid prepared by embodiment 1 in mosquito-expelling and antipruritic cream
Table 7
Table 8
Table 9
Table 10
| Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | |
| Number (antipruritic effect is commented) | 25 | 25 | 25 | 25 | 25 |
| Antipruritic rate | 100% | 100% | 100% | 100% | 100% |
Table 7 is application of the mosquito-expelling and antipruritic plant extraction liquid of the present invention in mosquito-expelling and antipruritic cream.Mosquito-expelling and antipruritic plant extract
Liquid has natural antiseptic property, adds in formula system, can substitute preservative and play antisepsis, pass through the knot of table 8
Fruit is it is found that antipruritic paste formulation 1~5 is tested by preservation challenge；Meanwhile antipruritic paste formulation 1~5 is high for mosquito mosquito repellent rate
Up to 100%, there is fabulous mosquito proof effect, the results are shown in Table 9.
Volunteer does not occur adverse reaction (such as facial redness, allergy etc.) during experiencing itching-relieving cream product, this
Itching-relieving cream product in invention is fabulous for antipruritic effect caused by after bite by mosquitos, and antipruritic rate 100%, the results are shown in Table
10。
Four, Application Example 11
The application of mosquito-expelling and antipruritic plant extraction liquid prepared by embodiment 1 in mosquito-expelling and antipruritic liquid detergent
Table 11
Table 12
| Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | |
| Whether tested by preservation challenge | Pass through | Pass through | Pass through | Pass through | Pass through |
Table 13
Table 11 is application of the mosquito-expelling and antipruritic plant extraction liquid of the present invention in liquid detergent.Mosquito-expelling and antipruritic plant extraction liquid has
There is natural antiseptic property, adds in formula system, preservative can be substituted and play antisepsis, it can by the result of table 12
Know, laundry liquid formulation 1~5 is tested by preservation challenge；Meanwhile laundry liquid formulation 1~5 is up to mosquito mosquito repellent rate
90%, there is fabulous mosquito proof effect, the results are shown in Table 13.
Five, Application Example 12
The application of mosquito-expelling and antipruritic plant extraction liquid prepared by embodiment 1 in mosquito-expelling and antipruritic baby Jinsui River
Table 14
Table 15
| Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | |
| Whether tested by preservation challenge | Pass through | Pass through | Pass through | Pass through | Pass through |
Table 16
Table 17
Table 14 is application of the mosquito-expelling and antipruritic plant extraction liquid of the present invention in baby Jinsui River.Mosquito-expelling and antipruritic plant extraction liquid
With natural antiseptic property, adds in formula system, preservative can be substituted and play antisepsis, pass through the result of table 15
It is found that baby Jinsui River formula 1~5 is tested by preservation challenge；Meanwhile baby Jinsui River formula 1~5 is for mosquito mosquito repellent rate
Up to 100%, there is fabulous mosquito proof effect, the results are shown in Table 16.
Volunteer does not occur adverse reaction (such as facial redness, allergy etc.) during the product of experience baby Jinsui River,
Baby Jinsui River product in the present invention is fabulous for antipruritic effect caused by after bite by mosquitos, and antipruritic rate 100%, is as a result shown in
Table 17.
Biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and the preparation method and application thereof provided by the invention
It has the advantages that：
One, for mosquito-expelling and antipruritic plant extraction liquid provided by the invention using Asian puccoon, honeysuckle flowers and camellia seed as raw material, utilization is compound
It is thin to crack plant under the first enzyme preparation and the collective effect of alcohols material comprehensively for the peculiar technology extraction active material of biological enzyme
The closed structure of cell wall and cytoplasm, the active material in high efficiency extraction raw material, and avoid using virose organic molten
Agent improves the safety of mosquito-expelling and antipruritic plant extraction liquid.
Two, the active material for effectively compounding the second enzyme preparation using ultrasonic wave and being extracted from raw material, makes the mosquito repellent of preparation
Antipruritic plant extraction liquid itself has antiseptic property, addition preservative is no longer needed to, on the one hand in cosmetics and personal care and toiletry
Using when can be to avoid negative effects such as the stimulations that bring of addition preservative, on the other hand can also extend product itself uses the longevity
Life.
Three, mosquito-expelling and antipruritic plant extraction liquid provided by the invention is soluble liquid, overcomes powdered moisturizing extract
The limitation of compliant applications in various product system, and mosquito-expelling and antipruritic plant extraction liquid provided by the invention is mildly stingless
Swash, antiallergy ability is strong.
Above-described is only embodiments of the present invention, it should be noted here that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, improvement can also be made, but these belong to the protection model of the present invention
It encloses.
Claims (9)
1. a kind of preparation method with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid, which is characterized in that the system
Preparation Method includes the following steps：
(1) 10~80 portions of Asian puccoons of raw material, 10~80 parts of honeysuckle flowers and 10~80 parts of camellia seeds are pre-processed to obtain length less than pre-
If the mixed plant piece of value；
(2) mixed plant piece made from step (1) is placed in 20~45 DEG C of soak and is impregnated 15~30 minutes, the immersion
Liquid includes 5~10 parts of the first enzyme preparation, 1~5 part of alcohols material and 40~100 parts of water, wherein first enzyme preparation is fibre
The mixture of one or more of the plain enzyme of dimension, pectase, zytase and hemicellulase, the alcohols material are 1,
The mixture of one or more of 2- hexylene glycols, butanediol, propylene glycol, pungent glycol；
(3) the mixed plant piece by soak and after impregnating is transferred in processor for ultrasonic wave, and 1 is ultrasonically treated at 20~45 DEG C
~2 hours, isolated supernatant after static layering；
(4) supernatant is heated 5~15 minutes at 85~100 DEG C, after being cooled to room temperature, adds 5~10 parts
Second enzyme preparation, and be ultrasonically treated 1~2 hour at 20~45 DEG C and obtain mixed liquor, wherein second enzyme preparation includes Portugal
Carbohydrate oxidase and/or lysozyme；
(5) mixed liquor removes impurity, the eluent of acquisition using the chromatography column pure water elution for being filled with resin extender
That is mosquito-expelling and antipruritic plant extraction liquid.
2. the preparation method according to claim 1 with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid,
It is characterized in that, the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is (0.25~2)：(0.5~8)：1.
3. the preparation method according to claim 2 with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid,
It is characterized in that, the weight ratio of the Asian puccoon, the honeysuckle flowers and the camellia seed is 2：1：1.
4. the preparation method according to claim 1 with biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid,
It is characterized in that, second enzyme preparation further includes Co-Q10 or protease.
5. a kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid, which is characterized in that the mosquito-expelling and antipruritic plant
Extracting solution uses the preparation method system of the mosquito-expelling and antipruritic plant extraction liquid of preservative free any one of Claims 1-4
It is standby to obtain.
6. application of the mosquito-expelling and antipruritic plant extraction liquid in preparing cosmetics and daily chemical product described in claim 5.
7. a kind of mosquito-expelling and antipruritic cream, which is characterized in that by its total weight be 100% in terms of comprising 5~15% claim 1
The mosquito-expelling and antipruritic plant extraction liquid being prepared to the preparation method described in any one of 4.
8. a kind of mosquito-expelling and antipruritic liquid detergent, which is characterized in that by its total weight be 100% in terms of comprising 0.1~15% right
It is required that the mosquito-expelling and antipruritic plant extraction liquid that the preparation method described in any one of 1 to 4 is prepared.
9. a kind of mosquito-expelling and antipruritic baby Jinsui River, which is characterized in that by its total weight be 100% in terms of comprising 3~18% right
It is required that the mosquito-expelling and antipruritic plant extraction liquid that the preparation method described in any one of 1 to 4 is prepared.
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| CN201810289710.9A CN108403493A (en) | 2018-03-30 | 2018-03-30 | A kind of biological enzyme processing and corrosion-resistant mosquito-expelling and antipruritic plant extraction liquid and the preparation method and application thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145657A (en) * | 2015-09-18 | 2015-12-16 | 崇州市地龙海龙生物制品开发研究所 | Environment-friendly mosquito repellent preparing method |
| CN107049869A (en) * | 2017-05-13 | 2017-08-18 | 蚌埠永济堂医药有限公司 | A kind of children special-purpose mosquito-repellent water |
| CN107823394A (en) * | 2017-12-16 | 2018-03-23 | 蒋央芳 | A kind of stop itching spray agent |
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2018
- 2018-03-30 CN CN201810289710.9A patent/CN108403493A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145657A (en) * | 2015-09-18 | 2015-12-16 | 崇州市地龙海龙生物制品开发研究所 | Environment-friendly mosquito repellent preparing method |
| CN107049869A (en) * | 2017-05-13 | 2017-08-18 | 蚌埠永济堂医药有限公司 | A kind of children special-purpose mosquito-repellent water |
| CN107823394A (en) * | 2017-12-16 | 2018-03-23 | 蒋央芳 | A kind of stop itching spray agent |
Non-Patent Citations (1)
| Title |
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| 罗永明 主编: "《中药化学成分提取分离技术与方法》", 31 January 2016, 上海科学技术出版社 * |
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