CN107049866A - A kind of antiallergy plant composition and its application - Google Patents
A kind of antiallergy plant composition and its application Download PDFInfo
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- CN107049866A CN107049866A CN201710291954.6A CN201710291954A CN107049866A CN 107049866 A CN107049866 A CN 107049866A CN 201710291954 A CN201710291954 A CN 201710291954A CN 107049866 A CN107049866 A CN 107049866A
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/33—Cactaceae (Cactus family), e.g. pricklypear or Cereus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/72—Hypo-allergenic
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Abstract
The invention discloses a kind of antiallergy plant composition and its application, belong to cosmetic technical field.Said composition includes dryopteris fragrans extract, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and licorice.Present invention also offers the application of the antiallergy plant composition.The raw material that the present invention is selected is natural component of releiving, and contains material needed for the Human Physiologies such as abundant amino acid, trace element and antianaphylaxis material.In addition, the present invention has prepared three sections of skin care item according to the need for people and conveniently, it is mildy wash, moisture retention water and moisturizer respectively, so that people's selection is used.
Description
Technical field
The present invention relates to a kind of antiallergy plant composition and its application, belong to cosmetic technical field.
Background technology
In recent years, with the exacerbation of environmental pollution, the abuse of all types of industries additive, and people's eating habit change
Etc. reason, autopath sharply increases and symptom is more intensification.According to incompletely statistics, the whole world feels sensitiveness skin
Crowd is also more and more, and wherein women accounts for 61%, and male accounts for 38%, the allergic major issue as puzzlement health
One of.Cutaneous anaphylaxis be also referred to as allergy or be hypersensitivity, it refers to body when by antigenic stimulus
The special immune response of a produced class, this kind of response can cause tissue damage and the disorder of immunologic function.Appear in people
Body on also known as allergic contact dermatitis, be characterized in itch, produce erythema, blister etc., it occurs to depend on following several
Individual factor：The stimulation of antigen, the reactive of body and other kinds of factor (environment, diet and medicine).
Meanwhile, with the continuous improvement of people's living standards, and pursuit of the people to high-quality life, cosmetics are
Become the necessity in people's life, such as fruit cosmetic can then produce some bad symptoms, such as skin mistake using improper
Quick, itch and redness etc., thus people more and more focus on the natural sex and the security of itself of cosmetics.Modern cosmetics are present
Many problems, for example, using violated additive, total number of bacteria is exceeded, heavy metal or harmful substance are exceeded, cosmetics sense organ
Index is not up to regulation requirement etc., and with certain toxic side effect.
Dryopteris fragrans (Dryopteris fragrans), are Dryopteridaceae Dryopteris, are a kind of wild perennial ferns
Class plant, medical value is very high, and great exploitation potential.Dryopteris fragrans are considered as " skin by the northern resident in Heilongjiang Province
The jinx of skin disease ", its decocting liquid energy effectively treats multiple dermatosis, such as：Chronic eczema, fash, acne, psoriasis etc..For many years
Human trial proves that dryopteris fragrans also have the double action cured the disease with beauty not only without toxic side effect.But it is relevant fragrant
The research of shield-fern antiallergic skin care item has not been reported.
Studies have found that having certain positive related between the antiallergy mechanism and free radical scavenging activity of plant extracts
Property, DPPH methods can be as a kind of method for evaluating natural antioxidation activity, and its feature is quick, easy, sensitive, can
OK.
Hyaluronic acid (Hyaluronic Acid, HA) has stronger water absorbing capacity and adhesiveness, can pin water in skin
Divide and the elasticity of skin can be maintained, may additionally facilitate wound healing etc..Hyaluronidase (Hyaluronidase, HAase) is transparent
The selectivity lyases of matter acid, only suppresses the activity of hyaluronidase, just can guarantee that hyaluronic acid can travel normal function.
It is reported that hyaluronidase participates in the anaphylactoid phase III, mast cell can be promoted degranulation phenomenon occur.Hyalomitome
It is to evaluate the most typical method of antiallergic activity, the more big then table of maximum inhibition of hyaluronidase that sour enzyme external activity, which suppresses experiment,
Show that anti-allergic effects are stronger.
The content of the invention
For allergic symptom of releiving, pursuit of the people to cosmetics " green, safety " is met, it is an object of the invention to provide
A kind of plant composition and its application for antianaphylactic skin care item, said composition includes dryopteris fragrans extract, radix scutellariae
Extract, Radix et Caulis Opuntiae Dillenii extract and licorice.
First purpose of the present invention is to provide a kind of antiallergy plant composition, and the antiallergy plant composition includes
Dryopteris fragrans extract, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and licorice.
Preferably, described antiallergy plant composition includes the raw material of following mass fraction：Dryopteris fragrans extract
0.025 part -0.1 part, 0.025 part -0.05 part of Baical Skullcap root P.E, 0.05 part -0.1 part of Radix et Caulis Opuntiae Dillenii extract, licorice
0.05 part -0.1 part.
It is highly preferred that described antiallergy plant composition, includes the raw material of following mass fraction：Dryopteris fragrans extract
0.1 part, 0.05 part of Baical Skullcap root P.E, 0.1 part of Radix et Caulis Opuntiae Dillenii extract, 0.1 part of licorice.
Preferably, the concentration of dryopteris fragrans extract of the present invention is 0.025mg/mL-0.1mg/mL；The radix scutellariae is carried
The concentration for taking thing is 0.025mg/mL-0.05mg/mL；The concentration of the Radix et Caulis Opuntiae Dillenii extract is 0.05mg/mL-0.1mg/mL；
The concentration of the licorice is 0.05mg/mL-0.1mg/mL.
It is highly preferred that the concentration of dryopteris fragrans extract of the present invention is 0.1mg/mL；The Baical Skullcap root P.E it is dense
Degree is 0.05mg/mL；The concentration of the Radix et Caulis Opuntiae Dillenii extract is 0.1mg/mL；The concentration of the licorice is 0.1mg/
mL。
The preparation method of dryopteris fragrans extract of the present invention is：Dry dryopteris fragrans powder 20.0g is weighed, by material
Liquor ratio is 1：The ratio of (5-25) (g/mL) adds the ethanol solution that volume fraction is 10%-90%, respectively in 20 DEG C of -60 DEG C of temperature
The lower ultrasonic extraction 10min-50min of degree, leaching liquor is condensed into medicinal extract after suction filtration.
Preferably, the preparation method of the dryopteris fragrans extract is：Dry dryopteris fragrans powder 20.0g is weighed, is pressed
Solid-liquid ratio is 1：The ratio of (5-15) (g/mL) adds the ethanol solution that volume fraction is 50%-90%, respectively at 30 DEG C -50 DEG C
At a temperature of ultrasonic extraction 30min-50min, leaching liquor is condensed into medicinal extract after suction filtration.
It is highly preferred that the preparation method of the dryopteris fragrans extract is：Dry dryopteris fragrans powder 20.0g is weighed,
It is 1 by solid-liquid ratio:10 (g/mL) ratio adds the ethanol solution that volume fraction is 90%, and ultrasound is carried at a temperature of 50 DEG C respectively
30min is taken, leaching liquor is condensed into medicinal extract after suction filtration.
The preparation method of Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and licorice is as follows in composition of the present invention：
The powder 20.0g of radix scutellariae or cactus or radix glycyrrhizae is weighed, by solid-liquid ratio 1:20g/mL is added to the ethanol that volume fraction is 80%
In solution, in merging filtrate after progress ultrasonic extraction 2 times, each ultrasonic extraction time 30min, suction filtration under 800W power, it is used in combination
Rotary Evaporators are concentrated, and are prepared into medicinal extract standby.
Second object of the present invention is to provide application of the antiallergy plant composition in skin care item are prepared.
Addition 0.7g-3.5g institutes in a kind of skin care item containing above-mentioned antiallergy plant composition, skin care item described in per 100mL
State antiallergy plant composition.
Preferably, antiallergy plant composition described in addition 2.1g in skin care item described in every 100mL.
Preferably, skin care item of the present invention are mildy wash, moisture retention water or moisturizer.
Preferably, the manufacture craft when skin care item are mildy wash comprises the following steps：
1) by dodecyl ether sulfate ammonium, alkyl polyglycoside, fatty acid distribution of coconut oil diglycollic amide, cocamidopropyl propyl amide beet
Alkali, cocounut oil acid glyceride, glycerine, citric acid, deionized water are placed in beaker, are slowly stirred heating, until being heated to 75 DEG C,
Stir to transparent；
2) whne step 2) when being cooled to 45 DEG C, add claim 1 described in antiallergy plant composition, stir, then
Essence and preservative are added, is stirred, moisturizing；
3) with the viscosity of sodium chloride regulation system untill, after cooling in canned to sterile Packaging Bottle；
The manufacture craft when skin care item are moisture retention water comprises the following steps：
1) glycerine, butanediol, sorbierite, allantoin, water-soluble jojoba oil and deionized water are placed in beaker, heated up
To 80 DEG C -85 DEG C, stirring and dissolving；
2) step 1 is treated) 50 DEG C are cooled to, antiallergy plant composition described in addition claim 1 stirs；
3) by step 2) temperature be down to less than 40 DEG C, add hyaluronic acid and MTI, stir, moisturizing, tank after cooling
It is filled in sterile Packaging Bottle；
The manufacture craft when skin care item are moisturizer comprises the following steps：
1) cetostearyl alcohol/cetearyl glucoside, stearine/PEG-100 stearates, poly- diformazan are weighed
Radical siloxane, methyl hydroxybenzoate, propylben and ethylhexyl palmitate are added in 100mL beakers, are warming up to 80 DEG C -85
DEG C, stirring and dissolving obtains solution A；
2) 200mL beakers are selected, first disperse carbomer and xanthan gum with butanediol, then add EDETATE SODIUM, allantoin,
Propane diols and deionized water, are warming up to 80 DEG C -85 DEG C, stirring fully dissolving obtains solution B；
3) by step 1) obtain solution A be poured slowly into step 2) obtain solution B in, emulsifying homogeneous, homogeneous 6min, turn
Speed is 3000r/min；
4) step 3) after the completion of homogeneous, emulsion is stirred and cooled, 60 DEG C of addition triethanolamines are down to, are stirred；
5) step 4) it is cooled to 40 DEG C and adds aloe gels, hyaluronic acid, the antiallergy plant society described in claim 1
Thing, Sensiva SC50, stimulation inhibiting factor, avenabeta glucosan, are stirred, and PH is adjusted to 5.5-6.5 with triethanolamine, cold
But after in canned to sterile Packaging Bottle.
Avenabeta glucosan of the present invention is a kind of commercial prod, includes water, glycerine and beta glucan.
Mildy wash prepared by the present invention is fine and smooth milky lotion, the characteristic odor containing Chinese herbal medicine.
Moisture retention water prepared by the present invention is light yellow clear liquid, the characteristic odor containing Chinese herbal medicine.
Moisturizer prepared by the present invention is milky thick liquid, the characteristic odor containing Chinese herbal medicine.
Studies have found that having certain positive related between the antiallergy mechanism and free radical scavenging activity of plant extracts
Property, DPPH methods can be as a kind of method for evaluating natural antioxidation activity, and its feature is quick, easy, sensitive, can
OK.Therefore, to remove the anti-of a kind of DPPH this antioxidant system research dryopteris fragrans, radix glycyrrhizae, cactus and Baical Skullcap root P.E
Oxidability, the reciprocation between them is studied using orthogonal design, and the experimental result according to DPPH methods determines level, adopts
The oxidation resistance of composition is determined with DPPH methods, so as to obtain the composition ratio of best results.The optimal compound of the present invention
The consumption of composition is 2.1%, and dryopteris fragrans, radix scutellariae, the amount ratio of cactus and licorice are 2:1:2:2, by compound
Composition is added in skin care item matrix, making anti-allergic skin care product, such as mildy wash, moisture retention water or moisturizer, and then by anti-
Quick experimental verification compound composition has certain antiallergic effect, and the optimal addition of compound composition is determined is
2.1% (g/mL), its antiallergic effect is better than positive reference substance Symsitive1609.
Beneficial effect of the present invention：
The raw material that the present invention is selected is natural component of releiving, action temperature and.The antiallergic effect of compound is notable,
It is added in particular substrate, develops natural antiallergic skin care item, has met modern's pursuit " green ", the need of " natural "
Ask.This research is not only that antiallergic effect of research natural plants provides foundation, but also meets people to skin care quality
With the requirement of performance, antiallergic skin care item related to exploitation have directive significance.In addition, the present invention is by reasonably matching so that
The skin care item product produced, is not only outward appearance or its quality keeps stable and safe and reliable, makes us being easy to receive.
Chinese herbal medicine cosmetic refers to be made up of Chinese herbal medicine, or in specific matrix system add Chinese herbal medicine active component
Cosmetics.However, the cosmetics for adding plant material simplely in prescription and being fabricated to, it is impossible to be called Chinese herbal medicine
Cosmetic.Process for preparation should follow the principles of formulating prescriptions of traditional Chinese medicine " monarch ", then be formed with the cosmetics of specific function, this
The security of class cosmetic product is higher, and the effect that can not be compared with single Chinese herbal medicine.Skin care item are the one of cosmetics
Kind.Thus research and develop the antiallergy plant composition of skin care item, should suit the requirement of traditional Chinese medical theory, embody " monarch and
The thought of humorous compatibility ".And consumer of today also increasingly payes attention to the intension and theory of skin care item, therefore by " monarch "
Thought is merged in antiallergic skin care item, can not only be explained from mechanism, and be easily esthetically acceptable to the consumers.Compound composition
Effect, mainly determined by effect and compatibility relationship for constituting flavour of a drug.In monarch, monarch drug in a prescription as main composition,
Effect with antiallergic；Ministerial drug plays auxiliary, then with antibacterial effect；Adjutant is then the effect for promoting monarch drug in a prescription and ministerial drug
Play, the effect with antiallergic and Shu Min；Make composition based on medicine, play a part of coordinating the drug actions of a prescription and moisturizing.So, tentatively
It is monarch drug in a prescription to determine dryopteris fragrans, and radix scutellariae is ministerial drug, and cactus is adjutant, and radix glycyrrhizae is to make medicine.
In composite skin care product of the present invention, this 4 kinds of Chinese herbal medicines of dryopteris fragrans, radix scutellariae, cactus and the radix glycyrrhizae used
Extract plays the effect such as antiallergic, antibacterial, Shu Min, moisturizing respectively, promotes various medicinal herb componentses to adjust mutually, and collaboration mutually is made
With so as to reach antianaphylactic resultant effect.
The antiallergy plant composition that the present invention is provided, which can be added in particular substrate, is prepared into Chinese herbal medicine cosmetic, in
Chinese herbal medicine additive agent it is safe, matrix is nontoxic, and with specific function, is added among cosmetics, and it is pierced
Sharp property is small, safe, curative effect is further obvious.
Hyaluronidase body outer suppressioning test is to evaluate the most typical method of antiallergic activity, to the inhibiting rate of hyaluronidase
It is more big then to represent that anti-allergic effects are stronger.Using hyaluronic acid enzyme inhibition rate as antianaphylactic standard, anti-allergic effects are divided into 4
Individual grade：Inhibiting rate ＜ 30% is without anti-allergic effects；30%≤inhibiting rate ＜ 50% is weak anti-allergic effects；Inhibiting rate >=
50% is medium anti-allergic effects；Inhibiting rate >=70% is strong anti-allergic effects.Experiment shows, dryopteris fragrans extract, radix scutellariae
Extract, Radix et Caulis Opuntiae Dillenii extract and licorice are respectively provided with inhibition to hyaluronidase, and inhibiting rate is above 70%, tool
There are strong anti-allergic effects.The antiallergy size of these four Chinese herbal medicine extracts is followed successively by：Dryopteris fragrans (inhibiting rate 90.4%) ＞
Radix glycyrrhizae (inhibiting rate 88.3%) ＞ cactuses ＞ (inhibiting rate 83.3%) ＞ radix scutellariaes (78.24%).Dryopteris fragrans of the present invention are extracted
Thing, Baical Skullcap root P.E, the complex composition thing of Radix et Caulis Opuntiae Dillenii extract and licorice are to the maximum inhibition of hyaluronidase
Up to more than 90%, its antiallergic effect is better than positive reference substance Symsitive1609 (70.8%), and antiallergy advantage is more bright
It is aobvious.
When body produces allergic reaction, if the bacterium concurrent infection with skin surface, can increase in cutaneous anaphylaxis
Existing dermal pathology damage, so the raw material in composition is on the basis of antianaphylactic, in addition it is also necessary to skin surface can be suppressed normal
Several bacterium seen, such as staphylococcus aureus, MRSE and Malassezia furfur etc..Experiment shows, in these four
In herb extracts, the fungistatic effect of dryopteris fragrans extract and Baical Skullcap root P.E is preferable.
Brief description of the drawings
Fig. 1 is influence of the solid-liquid ratio to hyaluronidase activity inhibiting rate.
Fig. 2 is influence of the ultrasonic temperature to hyaluronidase activity inhibiting rate.
Fig. 3 is influence of the ultrasonic time to hyaluronidase activity inhibiting rate.
Fig. 4 is influence of the volume fraction of ethanol to hyaluronidase activity inhibiting rate.
Fig. 5 is antiallergic effect of 4 kinds of Chinese herbal medicine extracts.
Fig. 6 is scavenging action of 4 kinds of Chinese herbal medicine extracts to DPPH.
Fig. 7 is the antiallergic result of compound composition.
Embodiment
Technical scheme is illustrated with reference to embodiment, but following examples are not constituted to this hair
Bright restriction.
" % " represents " weight % " in following examples.
MATERIALS METHODS in following examples is as follows：
Test material：
Dryopteris fragrans pick up from the lava plateau of Wudalianchi Area, Heilongjiang Province；Radix glycyrrhizae, radix scutellariae and cactus are purchased from black
The tree Chinese Medicinal Materials Markets of Longjiang Harbin City of province three.
Embodiment 1：The optimization of dryopteris fragrans technology for extracting effective component
1st, influence of the solid-liquid ratio to hyaluronidase activity inhibiting rate
5 parts dry of dryopteris fragrans powder 20.0g is weighed, is respectively 1 by solid-liquid ratio:5g/mL、1:10g/mL、1:15g/
mL、1:20g/mL、1:25g/mL ratio adds the ethanol solution of volume fraction 70% (v/v), surpasses respectively at a temperature of 30 DEG C
Sound extracts 30min, and leaching liquor is condensed into medicinal extract after suction filtration.Medicinal extract is dissolved into 1% alcoholic solution respectively, then carried out transparent
Matter acid enzyme level experiment, investigates influence of the solid-liquid ratio to hyaluronidase activity inhibiting rate, as a result sees Fig. 1.As shown in Figure 1, with
The increase of solid-liquid ratio, dryopteris fragrans extract suppresses to take the lead in decline after increase to hyaluronidase activity, when solid-liquid ratio is 1:
When 10, dryopteris fragrans extract reaches maximum (85.19%) to the maximum inhibition of hyaluronidase.Therefore, optimum extraction material
Liquor ratio is 1:10g/mL, the level for determining solid-liquid ratio influence factor in orthogonal test is 1:5g/mL-1:15g/mL.
2nd, influence of the ultrasonic temperature to hyaluronidase activity inhibiting rate
5 parts dry of dryopteris fragrans powder 20.0g is weighed, volume fraction 70% (v/v) ethanol solution is separately added into
300mL, respectively the ultrasonic extraction 30min at a temperature of 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C, leaching liquor are condensed into after suction filtration
Medicinal extract.Medicinal extract is dissolved into 1% alcoholic solution respectively, hyaluronic acid enzyme level experiment is then carried out, ultrasonic temperature is investigated to saturating
The influence of bright matter phytase activity inhibiting rate, is as a result shown in Fig. 2.As shown in Figure 2, when ultrasonic temperature is 30 DEG C, dryopteris fragrans are extracted
Thing reaches maximum (85.59%) to the maximum inhibition of hyaluronidase, and antiallergic effect is best.But with the rise of temperature,
The antiallergic effect of extract is reduced on the contrary, it may be possible to because temperature is too high, and the magazine of dissolution increases in the presence of ultrasonic wave,
Antiallergic effect is caused to decline, it is also possible to which temperature is too high, some heat-sensitive ingredients are destroyed and lost.Therefore, it is optimal super
Sound temperature is 30 DEG C, it is contemplated that antianaphylactic standard, and the level for determining ultrasonic temperature influence factor in orthogonal test is 30 DEG C -50
℃。
3rd, influence of the ultrasonic time to hyaluronidase activity inhibiting rate
5 parts dry of dryopteris fragrans powder 20.0g is weighed, volume fraction 70% (v/v) ethanol solution is separately added into
300mL, respectively ultrasonic extraction 10min, 20min, 30min, 40min, 50min at a temperature of 30 DEG C, leaching liquor are dense after suction filtration
Shorten medicinal extract into.Medicinal extract is dissolved into 1% alcoholic solution respectively, hyaluronic acid enzyme level experiment is then carried out, ultrasonic time is investigated
Influence to hyaluronidase activity inhibiting rate, is as a result shown in Fig. 3.From the figure 3, it may be seen that the time of ultrasound extraction is in 10min-40min
Interior, with the extension of ultrasonic time, the maximum inhibition of hyaluronidase gradually increases, during 40min, dryopteris fragrans extract pair
The maximum inhibition of hyaluronidase reaches maximum (84.75%).Continue to extend ultrasonic extraction time, the suppression to hyaluronidase
Effect processed is on a declining curve on the contrary, and this is probably the long structure for destroying active ingredient of ultrasonic time.Therefore, during optimal ultrasound
Between be 40min, determine ultrasonic time influence factor in orthogonal test level be 30min-50min.
4th, influence of the volume fraction of ethanol to hyaluronidase activity inhibiting rate
5 parts dry of dryopteris fragrans powder 20.0g is weighed, 10% (v/v), 30% (v/v), 50% (v/ is separately added into
V), 70% (v/v), 90% (v/v) ethanol solution 300mL, respectively the ultrasonic extraction 30min at a temperature of 30 DEG C, leaching liquor warp
Medicinal extract is condensed into after suction filtration.Medicinal extract is dissolved into 1% alcoholic solution respectively, hyaluronic acid enzyme level experiment is then carried out, investigated
Influence of the volume fraction of ethanol to hyaluronidase activity inhibiting rate, is as a result shown in Fig. 4.As shown in Figure 4, with volume fraction of ethanol
Increase, dryopteris fragrans extract suppresses to take the lead in decline after increase to hyaluronidase activity, right when volume fraction of ethanol is 70%
The maximum inhibition of hyaluronidase reaches maximum (85.52%).Therefore, the optimal volume fraction of ethanol of extraction is 70%.Greatly
After 70%, inhibiting rate slightly declines, it can thus be seen that volume fraction of ethanol is too high and too low can reduce dryopteris fragrans
Antiallergic effect, the level for thereby determining that volume fraction of ethanol influence factor in orthogonal test is 50%-90%.
5th, orthogonal test：
The factor of table 1 and level
According to the result of above-mentioned experiment of single factor, choose significant in each factor of influence dryopteris fragrans antiallergic effect
Level carries out orthogonal test, and carries out range analysis to result, to determine the optimum process condition of ultrasonic extraction.Factor level table
It is shown in Table 1.Dryopteris fragrans powder 20.0g is weighed, according to L9(34) orthogonal array setting condition carry out ultrasonic extraction, with hyalomitome
The maximum inhibition of sour enzyme is index, the results are shown in Table 2.It can show that each factor is to hyalomitome by the range analysis result of table 2
The influence size order of phytase activity inhibiting rate is followed successively by：Solid-liquid ratio (A) ＞ volume fraction of ethanol (D) ＞ ultrasonic times (C) ＞
Ultrasonic temperature (B).In experimental design
The orthogonal experiments of table 2
Align the checking of knot fruit：
The process combination obtained under above-mentioned condition and the optimum combination A in the orthogonal test of table 22B3C1D3It is inconsistent, therefore root
According to optimum combination A2B3C1D3Condition carried out 3 checking tests, the results are shown in Table 3.As shown in Table 3, in optimization of orthogonal test group
Close A2B3C1D3Under the conditions of, extract is 91.25% to the average inhibition of hyaluronidase activity, higher than the inhibiting rate highest of table 2
Combine A2B1C2D3, meanwhile, its RSD=1.53% (n=3) shows this stable process conditions, feasible, therefore A2B3C1D3It is auxiliary for ultrasound
Help the optimum process condition for extracting dryopteris fragrans active ingredient.
The checking test result (n=3) of table 3
Embodiment 2：The research of 4 kinds of Chinese herbal medicine extract antiallergic effects
Take 0.1mL 0.25mmol/L CaCl2Solution and 0.5mL hyaluronic acid enzyme liquids, 37 DEG C of water-baths are incubated 20min；Plus
It (is respectively dryopteris fragrans extract solution, radix scutellariae extract solution, cactus extraction and licorice extract, concentration is to enter sample liquid
1.0% alcoholic solution, the volume fraction of ethanol is 5%) 0.5mL, and 37 DEG C of water-baths are incubated 20min；Add Sodium Hyaluronate liquid
0.5mL, 37 DEG C of water-baths are incubated 30min, room temperature 5min；0.4mol/L NaOH solution 0.1mL are added, acetylacetone,2,4-pentanedione is added
Solution 0.5mL, boiling water bath heating 15min, cools down 5min with frozen water immediately after；Add 1.0mL Ehrlich's reagents, 3.0mL
Absolute ethyl alcohol, develop the color 20min, using deionized water as control, determines its absorbance at 539mn.
Hyaluronic acid enzyme inhibition rate (%)=[(A-B)-(C-D)]/(A-B) × 100%
In formula：A --- contrast solution ABS values (replace sample solution) with 5% ethanol solution
B --- control blank solution ABS values (replace sample solution respectively with 5% ethanol solution and hac buffer
And enzyme liquid)
C --- sample solution ABS values
D --- sample blank solution A BS values (replacing enzyme liquid with hac buffer)
Evaluation result such as Fig. 5 institute of the 4 kinds of Chinese herbal medicine extracts that this experiment is used to hyaluronidase extracorporeal inhibiting rate
Show, the concentration of extract is 1.0%, and data are three average values.As a result show, 4 kinds of Chinese herbal medicine extracts are to hyaluronidase
Activity be respectively provided with inhibition, 70% is above to the extracorporeal inhibiting rate of hyaluronidase, stronger antiallergy effect is shown
Really, dipotassium glycyrrhizinate is as positive control, and the inhibiting rate of its hyaluronidase is up to 97.5%.These four Chinese herbal medicine extracts
Antiallergy size is followed successively by：Dryopteris fragrans (inhibiting rate 90.4%) ＞ radix glycyrrhizaes (inhibiting rate 88.3%) ＞ cactus ＞ (inhibiting rates
83.3%) ＞ radix scutellariaes (78.24%).
Embodiment 3：4 kinds of Chinese herbal medicine extracts suppress the research of skin bacteria
1) activation of strain：Chosen bacterium with collarium is connect and rule on flat board, be put into incubator and be inverted culture (bacterium
37 DEG C of culture 24h；28 DEG C of culture 48h of fungi.Grow after bacterium colony, plate streaking is activated picking single bacterium colony again, in principle, flat board
It is the activation that can reach strain that line, which activates three times,.The strain activated is taken, aseptically, with connecing one bacterium of collarium picking
Fall, be added in fluid nutrient medium, be put into shaking table, Bacteria Culture 12h-14h, cultivation temperature is 37 DEG C, fungal culture 3d-5d,
Cultivation temperature is 28 DEG C.
2) preparation of bacteria suspension：By the bacterium solution after culture, diluted using 10 times of dilution methods with fluid nutrient medium, being made one is
Row bacteria suspension, is counted using blood counting chamber, and the concentration of bacteria suspension is adjusted into 106cfu/mL。
3) measure (Odontothrips loti) of inhibitory effect：Bacterium solution is got on flat board, smears equal in planar surface with spreading rod
It is even, plate is then covered, 5min is dried.4 Oxford cups, a control group, three are put on one flat board of each sample, a flat board
Individual experimental group, using the solution of n.s as blank control group, have sample as experimental group, in each cup plus 200 μ L samples
(it is respectively dryopteris fragrans extract solution, radix scutellariae extract solution, cactus extraction and licorice extract, the concentration of extract solution is
100mg/mL).At a suitable temperature after culture certain time, the diameter of inhibition zone is determined, unit is mm.
When the diameter of inhibition zone is less than or during equal to 8mm, no bacteriostasis；When antibacterial circle diameter is more than 8mm, there is antibacterial
Effect, inhibition zone is bigger to represent that bacteriostasis is stronger；Meeting 3 repetition experiments has inhibition zone appearance, just qualified；It is negative right
Inhibition zone can not be produced according to group, the failure of an experiment is not so represented.Experimental result is shown in Table 4.As seen from table, dryopteris fragrans, radix glycyrrhizae, radix scutellariae
There is certain inhibitory action to 3 kinds of common skin bacterium with Radix et Caulis Opuntiae Dillenii extract, the preferable plant material of fungistatic effect is androconium hair
Fern and radix scutellariae.
44 kinds of Chinese herbal medicine extracts of table are to 3 kinds of dermopathic inhibitions (the diameter mm of inhibition zone)
Note：What three repetitions were tested is averaged；"-" indicates no fungistatic effect.
Embodiment 4：The design of compound composition
The preparation of sample：Dryopteris fragrans, radix glycyrrhizae, radix scutellariae and the extract of cactus (medicinal extract) each 1.0g are weighed, 5% second is used
Alcohol is settled in 100mL volumetric flasks after fully dissolving respectively, as 1% mother liquor, is diluted to required concentration during experiment again.
Remove DPPH free radicals：
Take 3mL sample solution and 3mL 2 × 10-4Mol/L DPPH solution is placed in a test tube, is shaken up (A1 pipes)；Take
The ethanol of 3mL 5% and 3mL 2 × 10-4Mol/L DPPH solution, shakes up (A2 pipes)；3mL absolute ethyl alcohols and 3mL sample solutions are taken,
Shake up (A3 pipes).Room temperature lucifuge reacts 30min, takes the ethanol of 3mL 5% and 3mL absolute ethyl alcohols, mixing shakes up, and then blank is adjusted
Zero, the absorbance of A1 pipes, A2 pipes and A3 pipes is surveyed under 517nm with ultraviolet specrophotometer, each sample is parallel to be done 3 times.
DPPH clearance rates (%)=[(A2+A3)-A1]/A2, make nonlinear fitting to sample concentration with clearance rate, are asked by matched curve
Obtain IC50Value.IC50 values are smaller, represent that sample Scavenging ability is stronger, its oxidation resistance is stronger.Experimental result is shown in Fig. 6,
DPPH clearance rates are shown in Table 5 to the relation of concentration.Four kinds of extracts can remove DPPH it can be seen from Fig. 6 and table 5,
It is suitable with Vc effect during high concentration.In experiment, the order that they remove DPPH capacity of water is：Vc ＞ dryopteris fragrans ＞ is yellow
A kind of reed mentioned in ancient books ＞ radix glycyrrhizae ＞ cactuses, test result indicates that, dryopteris fragrans, radix scutellariae, cactus and licorice are good anti-oxidant
Agent.
Scavenging action of the 54 kinds of Chinese herbal medicine extracts of table to DPPH
Orthogonal Experiment and Design：
It is monarch drug in a prescription to primarily determine that dryopteris fragrans, and radix scutellariae is ministerial drug, and cactus is adjutant, and radix glycyrrhizae is to make medicine.In orthogonal test
In, determination oxidative is carried out to formula (antiallergy plant composition) using DPPH methods, therefore according to above-mentioned DPPH methods
Experimental result determines level.Factor level table and orthogonal experiments are shown in Table 6 and table 7 respectively.
The factor of table 6 and level
The orthogonal experiments of table 7
It can be drawn by the result of the range analysis of table 7, influence order of each factor to DPPH clearance rates is followed successively by：
Dryopteris fragrans (A) ﹥ radix scutellariae (B) ﹥ radix glycyrrhizae (D) ﹥ cactuses (C).In the range of experimental design, obtained formula combination is
A3B3C3D3, i.e., concentration of each extract in antiallergy plant society composition formula is：The concentration of dryopteris fragrans extract is
0.1mg/mL, the concentration of Baical Skullcap root P.E are that 0.05mg/mL, the concentration of Radix et Caulis Opuntiae Dillenii extract are 0.1mg/mL and the extraction of radix glycyrrhizae
The concentration of thing is 0.1mg/mL, due to sample liquid (dryopteris fragrans extract, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and radix glycyrrhizae
Extract composition composition) cumulative volume be 3mL, i.e. dryopteris fragrans extract, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and Gan
The mass ratio of the extract of grass is 0.3:0.15:0.3:0.3, i.e., 2:1:2:2.
Align the checking of knot fruit：
The above-mentioned formula combination preferably gone out is subjected to 3 parallel tests, the average clearance rate for obtaining DPPH is
92.56%, its RSD=0.116% (n=3), illustrating the formula combination, not only clearance rate is high, and the favorable reproducibility of experiment.
Embodiment 5：The determination of compound composition efficacy validation and formula
Present embodiments provide and contain dryopteris fragrans extract 3g, Huang in a kind of antiallergy plant composition, said composition
A kind of reed mentioned in ancient books extract 1.5g, Radix et Caulis Opuntiae Dillenii extract 3g and the extract 3g of radix glycyrrhizae (dryopteris fragrans extract, Baical Skullcap root P.E, cactus
Extract and the extract quality of radix glycyrrhizae are than 2:1:2:2), by dryopteris fragrans extract, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract
It is well mixed with the extract of radix glycyrrhizae and obtains antiallergy plant composition.
The preparation method of dryopteris fragrans extract is：Dry dryopteris fragrans powder 20.0g is weighed, is 1 by solid-liquid ratio：10
(g/mL) ratio adds the ethanol solution that volume fraction is 90%, respectively ultrasonic extraction 30min, leaching liquor at a temperature of 50 DEG C
Medicinal extract is condensed into after suction filtration.
The preparation method of Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and licorice is as follows：Weigh cactus or radix scutellariae or
The powder 20.0g of radix glycyrrhizae, by solid-liquid ratio 1:20g/mL is added in the ethanol solution that volume fraction is 80%, under 800W power
Merging filtrate after progress ultrasonic extraction 2 times, each ultrasonic extraction time 30min, suction filtration, and concentrated with Rotary Evaporators, prepare
It is standby into medicinal extract.
Antiallergy plant composition 0.7g, 1.4g, 2.1g, 2.8g, 3.5g of above-mentioned preparation is taken to be added to 100mL's respectively
In moisture retention water, test to verify the antianaphylactic ability of compound composition using hyaluronic acid enzyme level, experimental result is shown in
Fig. 7 and table 8.
The formula and preparation method of compound antiallergic moisture retention water are as follows：
Formula：Contain in per 100mL moisture retention waters：0.7%th, 1.4%, 2.1%, 2.8%, 3.5% antiallergy plant society
Thing, glycerine 5.0%, butanediol 2.0%, sorbierite 3.0%, allantoin 0.2%, water-soluble jojoba oil 0.2%, hyaluronic acid
2.0%th, MTI 0.15%, appropriate deionized water；
Manufacture craft is as follows：
1) glycerine, butanediol, sorbierite, allantoin, water-soluble jojoba oil and deionized water are added to 100mL beakers
In, it is warming up to 80~85 DEG C, stirring and dissolving；
2) step 3 is treated) 50 DEG C are cooled to, antiallergy plant composition is added, is stirred；
3) by step 4) temperature be down to less than 40 DEG C, add hyaluronic acid and MTI, stir, moisturizing, tank after cooling
It is filled in sterile Packaging Bottle.
The antiallergic result of the compound composition of table 8
As can be seen that being continuously increased with concentration, activity of the compound composition to hyaluronidase from Fig. 7 and table 8
Inhibiting rate is also gradually increasing, under same concentrations, and its antiallergic effect is better than positive reference substance Symsitive1609, anti-mistake
Quick advantage is more obvious.When addition of the composition in formula is 0.7%, the inhibiting rate to hyaluronidase is 50.88%,
With middle antiallergic effect, when addition of the composition in formula is 3.5%, the inhibiting rate to hyaluronidase is 92.98%,
With strong antiallergic effect.According to experimental result, and integrated economics benefit, it is determined that the addition of compound composition is 2.1%
(inhibiting rate to hyaluronidase is 90.79%), i.e., each extractive content in formula per 100g：Dryopteris fragrans 0.6g, radix scutellariae
0.3g, cactus 0.6g, radix glycyrrhizae 0.6g.
Embodiment 6
A kind of mildy wash for adding antiallergy plant composition is present embodiments provided, formula and preparation method are as follows：
Raw material and weight ratio in its formula are respectively：Antiallergy plant composition 2.1% (dryopteris fragrans, radix scutellariae, celestial being
The consumption of the palm and licorice is 2:1:2:2), dodecyl ether sulfate ammonium 15.0%, alkyl polyglycoside 5.0%, cocounut oil fat
Fat acid diglycollic amide 3.5%, Cocoamidopropyl betaine 4.0%, cocounut oil acid glyceride 0.4%, glycerine 3.0%, lemon
Lemon acid 0.13%, sodium chloride 0.8%, essence 0.2%, preservative 0.08%, appropriate deionized water.Manufacture craft is as follows：
1) preparation of dryopteris fragrans extract：Weigh dryopteris fragrans powder 20.0g, 1:10 solid-liquid ratio adds 90% second
After alcohol, lucifuge cold soaking 24h, ultrasonic wave added extracts merging filtrate after 30min, suction filtration under the conditions of 50 DEG C, 800W, and filtrate is rotated
Evaporation, is prepared into medicinal extract standby；
2) preparation of radix scutellariae, cactus, licorice：Vegetable material powder 20.0g is weighed, by solid-liquid ratio 1:20 add
In the ethanol solution for being 80% to volume fraction, in progress ultrasonic extraction 2 times, each ultrasonic extraction time under 800W power
Merging filtrate after 30min, suction filtration, and concentrated with Rotary Evaporators, it is prepared into medicinal extract standby；
3) 200mL beaker weight is weighed, and is recorded；
4) dodecyl ether sulfate ammonium, alkyl polyglycoside, fatty acid distribution of coconut oil diglycollic amide, cocounut oil acyl are added in beaker
Amine CAB, cocounut oil acid glyceride, glycerine, citric acid, deionized water, are slowly stirred heating, until being heated to 75 DEG C,
Stir to transparent；
5) whne step 4) when being cooled to 45 DEG C, add the antiallergy plant composition, stir, add essence and
Preservative, stirs, moisturizing；
6) with the viscosity of sodium chloride regulation system untill, after cooling in canned to sterile Packaging Bottle.
Mildy wash product stability is investigated：
1) pH study on the stability：Mildy wash is adjusted to different pH value, 24h is deposited, is observed successively in 1h, 6h, 12h, 24h
The state of mildy wash, investigation the results are shown in Table 9.As a result find, under different pH value ranges, in stable condition, the color and luster of mildy wash
It is substantially unchanged with viscosity, show mildy wash steady quality.
2) room temperature stability is investigated：Mildy wash is deposited at room temperature 4 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day,
5 days, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, viscosity B coefficent) successively, examine
Examine and the results are shown in Table 10.As a result find, test and investigate by room temperature, at room temperature, Product Status is basicly stable for mildy wash, without exception
Phenomenon.
3) low-temperature stability is investigated：Mildy wash is deposited 4 weeks under the conditions of -5 DEG C of low temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, viscosity successively
Change), investigation the results are shown in Table 11.As a result find, investigated by low temperature test, at low temperature, Product Status is substantially steady for mildy wash
It is fixed, phenomenon without exception.
4) high-temperature stability is investigated：Mildy wash is deposited 4 weeks under the conditions of 40 DEG C of high temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, viscosity successively
Change), investigation the results are shown in Table 12.As a result find, test and investigate by high temperature, at high temperature, Product Status is substantially steady for mildy wash
It is fixed, phenomenon without exception.
5) cold and heat succeed each other study on the stability：By mildy wash, in 40 DEG C of high temperature, circulation places each between -5 DEG C of low temperature respectively
24h, is circulated 2 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day, the 5th day, the 1st week, the 2nd week observe successively its stability (color,
Smell, precipitation, pH value, viscosity B coefficent), investigation the results are shown in Table 13.As a result find, investigated by destructive test, mildy wash exists
Under conditions of colding and heat succeed each other, Product Status is basicly stable, phenomenon without exception.
The mildy wash pH study on the stability results of table 9
The mildy wash room temperature state study on the stability result of table 10
Table 11 mildy wash low temperature (- 5 DEG C) state stability investigates result
The mildy wash condition of high temperature of table 12 (40 DEG C) study on the stability result
The mildy wash of table 13 cold and heat succeed each other (- 5 DEG C -40 DEG C) state stability investigate result
Embodiment 7
Present embodiments provideA kind of moisture retention water for adding antiallergy plant composition, formula and preparation method are as follows：
Raw material and weight ratio in its formula are respectively：Antiallergy plant composition 2.1% (dryopteris fragrans, radix scutellariae, celestial being
The consumption of the palm and licorice is 2:1:2:2), glycerine 5.0%, butanediol 2.0%, sorbierite 3.0%, allantoin 0.2%,
Water-soluble jojoba oil 0.2%, hyaluronic acid 2.0%, MTI 0.15%, appropriate deionized water.Manufacture craft is as follows：
1) preparation of dryopteris fragrans extract：Weigh dryopteris fragrans powder 20.0g, 1:10 solid-liquid ratio adds 90% second
After alcohol, lucifuge cold soaking 24h, ultrasonic wave added extracts merging filtrate after 30min, suction filtration under the conditions of 50 DEG C, 800W, and filtrate is rotated
Evaporation, is prepared into medicinal extract standby；
2) preparation of radix scutellariae, cactus, licorice：Vegetable material powder 20.0g is weighed, by solid-liquid ratio 1:20 add
In the ethanol solution for being 80% to volume fraction, in progress ultrasonic extraction 2 times, each ultrasonic extraction time under 800W power
Merging filtrate after 30min, suction filtration, and concentrated with Rotary Evaporators, it is prepared into medicinal extract standby；
3) glycerine, butanediol, sorbierite, allantoin, water-soluble jojoba oil and deionized water are added to 100mL beakers
In, it is warming up to 80 DEG C -85 DEG C, stirring and dissolving；
4) step 3 is treated) 50 DEG C are cooled to, the antiallergy plant composition is added, is stirred；
5) by step 4) temperature be down to less than 40 DEG C, add hyaluronic acid and MTI, stir, moisturizing, tank after cooling
It is filled in sterile Packaging Bottle.
Moisture retention water product stability is investigated：
1) pH study on the stability：Moisture retention water is adjusted to different pH value, 24h is deposited, is observed successively in 1h, 6h, 12h, 24h
The state of moisture retention water, investigation the results are shown in Table 14.As a result find, by pH study on the stability, moisture retention water it is in stable condition, color and luster and
Clarity is substantially unchanged, shows moisture retention water steady quality.
2) room temperature stability is investigated：Moisture retention water is deposited at room temperature 4 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day,
5 days, that 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability successively is (color, smell, precipitation, pH value, electrical conductivity, solvable
Property solid content), investigation the results are shown in Table 15.As a result find, by room temperature test investigate, moisture retention water at room temperature, Product Status base
This stabilization, in the absence of layering and oil slick phenomenon, phenomenon without exception.
3) low-temperature stability is investigated：Moisture retention water is deposited 4 weeks under the conditions of -5 DEG C of low temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, conductance successively
Rate, soluble solid), investigation the results are shown in Table 16.As a result find, investigated by low temperature test, moisture retention water at low temperature, product
State is basicly stable, in the absence of layering and oil slick phenomenon, phenomenon without exception.
4) high-temperature stability is investigated：Moisture retention water is deposited 4 weeks under the conditions of 40 DEG C of high temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, conductance successively
Rate, soluble solid), investigation the results are shown in Table 17.As a result find, by high temperature test investigate, moisture retention water at high temperature, product
State is basicly stable, in the absence of layering and oil slick phenomenon, phenomenon without exception.
5) cold and heat succeed each other study on the stability：By moisture retention water, in 40 DEG C of high temperature, circulation places each between -5 DEG C of low temperature respectively
24h, is circulated 2 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day, the 5th day, the 1st week, the 2nd week observe successively its stability (color,
Smell, precipitation, pH value, electrical conductivity, soluble solid), investigation the results are shown in Table 18.As a result find, examined by destructive test
Examine, moisture retention water is under conditions of colding and heat succeed each other, and Product Status is basicly stable, in the absence of layering and oil slick phenomenon, phenomenon without exception.
The moisture retention water pH study on the stability results of table 14
The moisture retention water room temperature state study on the stability result of table 15
Table 16 moisture retention water low temperature (- 5 DEG C) state stability investigates result
The moisture retention water condition of high temperature of table 17 (40 DEG C) study on the stability result
The moisture retention water of table 18 cold and heat succeed each other (- 5 DEG C -40 DEG C) state stability investigate result
Embodiment 8
A kind of moisturizer for adding antiallergy plant composition is present embodiments provided, formula and preparation method are as follows：
Raw material and weight ratio in its formula are respectively：Antiallergy plant composition 2.1% (dryopteris fragrans, radix scutellariae, celestial being
The consumption of the palm and licorice is 2:1:2:2), cetostearyl alcohol/cetearyl heteroside 1.2%, stearine/
PEG-100 stearates 1.2%, dimethyl silicone polymer 5%, methyl hydroxybenzoate 0.2%, propylben 0.1%, palmitic acid
Ethylhexyl 5%, carbomer 0.1%, xanthan gum 0.05%, butanediol 4%, EDETATE SODIUM 0.05%, allantoin 0.2%, third
Glycol 3%, triethanolamine 0.1%, aloe gel 3%, hyaluronic acid 2%, Sensiva SC50 0.3%, stimulation inhibiting factor
0.5%th, avenabeta glucosan 1%, appropriate deionized water.Manufacture craft is as follows：
1) preparation of dryopteris fragrans extract：Weigh dryopteris fragrans powder 20.0g, 1:10 solid-liquid ratio adds 90% second
After alcohol, lucifuge cold soaking 24h, ultrasonic wave added extracts merging filtrate after 30min, suction filtration under the conditions of 50 DEG C, 800W, and filtrate is rotated
Evaporation, is prepared into medicinal extract standby；
2) preparation of radix scutellariae, cactus, licorice：Vegetable material powder 20.0g is weighed, by solid-liquid ratio 1:20 add
In the ethanol solution for being 80% to volume fraction, in progress ultrasonic extraction 2 times, each ultrasonic extraction time under 800W power
Merging filtrate after 30min, suction filtration, and concentrated with Rotary Evaporators, it is prepared into medicinal extract standby；
3) cetostearyl alcohol/cetearyl heteroside, stearine/PEG-100 stearates, poly- diformazan are weighed
Radical siloxane, methyl hydroxybenzoate and propylben are added in 100mL beakers, are warming up to 80 DEG C -85 DEG C, stirring and dissolving is obtained
Obtain solution A；
4) 200mL beakers are selected, first disperse carbomer and xanthan gum with butanediol, then add ethylhexyl palmitate,
EDETATE SODIUM, allantoin, propane diols and deionized water, are warming up to 80 DEG C -85 DEG C, stirring fully dissolving obtains solution B；
5) by step 3) obtain solution A be poured slowly into step 4) obtain solution B in, emulsifying homogeneous, homogeneous 6min, turn
Speed is 3000r/min；
6) step 5) after the completion of homogeneous, emulsion is stirred and cooled, 60 DEG C of addition triethanolamines are down to, are stirred；
7) step 6) it is cooled to 40 DEG C of addition aloe gels, hyaluronic acid, the antiallergy plant composition, ethylhexyls
Glycerine, stimulate inhibiting factor, avenabeta glucosan, stir, with triethanolamine adjust PH to 5.5-6.5, after cooling it is canned extremely
In sterile Packaging Bottle.
Moisturizer product stability is investigated：
1) pH study on the stability：Moisturizer is adjusted to different pH value, 24h is deposited, is observed successively in 1h, 6h, 12h, 24h
The state of moisturizer, investigation the results are shown in Table 19.As a result find, under different pH value ranges, in stable condition, the color and luster of moisturizer
It is substantially unchanged with viscosity, show moisturizer steady quality.
2) room temperature stability is investigated：Moisturizer is deposited at room temperature 4 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day,
5 days, 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability (color, smell, precipitation, pH value, viscosity, conductance successively
Rate), investigation the results are shown in Table 20.As a result find, test and investigate by room temperature, at room temperature, Product Status is stable, is as good as moisturizer
Chang Xianxiang.
3) low-temperature stability is investigated：Moisturizer is deposited 4 weeks under the conditions of -5 DEG C of low temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, that 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability successively is (color, smell, precipitation, pH value, viscous
Degree, electrical conductivity), investigation the results are shown in Table 21.As a result find, investigated by low temperature test, at low temperature, Product Status is steady for moisturizer
It is fixed, phenomenon without exception.
4) high-temperature stability is investigated：Moisturizer is deposited 4 weeks under the conditions of 40 DEG C of high temperature, in the 1st day, the 2nd day, the 3rd day,
4th day, the 5th day, that 1 week, 2 weeks, 3 weeks and 4 weeks observe its stability successively is (color, smell, precipitation, pH value, viscous
Degree, electrical conductivity), investigation the results are shown in Table 22.As a result find, test and investigate by high temperature, at high temperature, Product Status is steady for moisturizer
It is fixed, phenomenon without exception.
5) cold and heat succeed each other study on the stability：By moisturizer, in 40 DEG C of high temperature, circulation places each between -5 DEG C of low temperature respectively
24h, is circulated 2 weeks, in the 1st day, the 2nd day, the 3rd day, the 4th day, the 5th day, the 1st week, the 2nd week observe successively its stability (color,
Smell, precipitation, pH value, viscosity, electrical conductivity), investigation the results are shown in Table 23.As a result find, investigated by destructive test, moisturizer
Under conditions of colding and heat succeed each other, Product Status is stable, phenomenon without exception.
The moisturizer pH study on the stability results of table 19
The moisturizer room temperature state study on the stability result of table 20
Table 21 moisturizer low-temperature condition (- 5 DEG C) study on the stability result
The moisturizer condition of high temperature of table 22 (40 DEG C) study on the stability result
The moisturizer of table 23 cold and heat succeed each other (- 5 DEG C -40 DEG C) state stability investigate result
Embodiment 9：Safety evaluatio
1) technical indicator is detected：The mildy wash of the detection preparation of embodiment 6, the moisture retention water of the preparation of embodiment 7, embodiment 8 are made
The indexs such as standby sense organ, physics and chemistry, the health of moisturizing milk product, its testing result is shown in Table 24-26.From result, this three sections shields
The index of skin product product meets 2015《Cosmetics safety technical specification》In requirement standard, show this three sections of skin care item products
It is qualified.
The mildy wash technical indicator of table 24
The moisture retention water technical indicator of table 25
The moisturizer technical indicator of table 26
2) human skin patch：The experiment is to detect cosmetic product to human body skin with the presence or absence of potential bad anti-
Relatively simple, the safe and reliable method answered.Subject 32 is chosen, age composition and sex are determined at random, are met
2015《Cosmetics safety technical specification》In require standard；50mm is no more than from area2, depth about 1mm spot examination device；It is tested
Person's inner forearm skin is as the position of experiment, and skin is divided into two parts, and a part is to add in blank control, each patch device
25 μ L are without the skin care item of antiallergic composition, and a part is test section, and 25 μ L addition antiallergic compositions are added in each patch device
Skin care item, are fixed spot examination device with the adhesive tape of low sensitization, the patch test time is 48h；Stick after rear 48h, remove spot examination device,
Change of skin is observed after 30min and result is recorded.Experimental result is shown in Table 27-29.As can be seen from the table, 32 subjects smear
After mildy wash, moisture retention water and moisturizer containing combined extracts, human skin has no obvious erythema and damage, shows this three sections
Skin care item product is almost non-stimulated to skin, safe.
The mildy wash patch test results of table 27
The moisture retention water patch test results of table 28
The moisturizer patch test results of table 29
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this
The people of technology, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection of the present invention
What scope should be defined by claims is defined.
Claims (10)
1. a kind of antiallergy plant composition, it is characterised in that the antiallergy plant composition includes dryopteris fragrans extraction
Thing, Baical Skullcap root P.E, Radix et Caulis Opuntiae Dillenii extract and licorice.
2. antiallergy plant composition according to claim 1, it is characterised in that include the raw material of following mass fraction：
0.025 part -0.1 part of dryopteris fragrans extract, 0.025 part -0.05 part of Baical Skullcap root P.E, 0.05 part -0.1 of Radix et Caulis Opuntiae Dillenii extract
Part, 0.05 part -0.1 part of licorice.
3. antiallergy plant composition according to claim 1, it is characterised in that include the raw material of following mass fraction：
0.1 part of dryopteris fragrans extract, 0.05 part of Baical Skullcap root P.E, 0.1 part of Radix et Caulis Opuntiae Dillenii extract, 0.1 part of licorice.
4. antiallergy plant composition according to claim 1, it is characterised in that the preparation of the dryopteris fragrans extract
Method is：Dry dryopteris fragrans powder 20.0g is weighed, is 1 by solid-liquid ratio：The ratio of (5-25) (g/mL) adds volume fraction
For 10%-90% ethanol solution, the ultrasonic extraction 10min-50min at a temperature of 20 DEG C -60 DEG C respectively, leaching liquor is after suction filtration
It is condensed into medicinal extract.
5. antiallergy plant composition according to claim 1, it is characterised in that Baical Skullcap root P.E in the composition,
The preparation method of Radix et Caulis Opuntiae Dillenii extract and licorice is as follows：The powder 20.0g of radix scutellariae or cactus or radix glycyrrhizae is weighed, by material
Liquor ratio 1:20 (g/mL) are added in the ethanol solution that volume fraction is 80%, in carrying out ultrasonic extraction under 800W power 2 times, often
Merging filtrate after secondary ultrasonic extraction time 30min, suction filtration, and concentrated with Rotary Evaporators, it is prepared into medicinal extract standby.
6. application of the antiallergy plant composition in skin care item are prepared described in claim 1.
7. a kind of skin care item containing any antiallergy plant compositions of claim 1-5, it is characterised in that per 100mL
Antiallergy plant composition described in 0.7g-3.5g is added in the skin care item.
8. skin care item according to claim 7, it is characterised in that resist in skin care item described in per 100mL described in addition 2.1g
Allergy plant composition.
9. skin care item according to claim 7, it is characterised in that the skin care item are mildy wash, moisture retention water or moisturizer.
10. skin care item according to claim 9, it is characterised in that
The manufacture craft when skin care item are mildy wash comprises the following steps：
1) by dodecyl ether sulfate ammonium, alkyl polyglycoside, fatty acid distribution of coconut oil diglycollic amide, Cocoamidopropyl betaine,
Cocounut oil acid glyceride, glycerine, citric acid, deionized water are placed in beaker, are slowly stirred heating, until being heated to 75 DEG C, are stirred
Mix to transparent；
2) whne step 2) when being cooled to 45 DEG C, add claim 1 described in antiallergy plant composition, stir, add
Essence and preservative, stir, moisturizing；
3) with the viscosity of sodium chloride regulation system untill, after cooling in canned to sterile Packaging Bottle；
The manufacture craft when skin care item are moisture retention water comprises the following steps：
1) glycerine, butanediol, sorbierite, allantoin, water-soluble jojoba oil and deionized water are placed in beaker, are warming up to 80
DEG C -85 DEG C, stirring and dissolving；
2) step 1 is treated) 50 DEG C are cooled to, antiallergy plant composition described in addition claim 1 stirs；
3) by step 2) temperature be down to less than 40 DEG C, add hyaluronic acid and MTI, stir, moisturizing, after cooling it is canned extremely
In sterile Packaging Bottle；
The manufacture craft when skin care item are moisturizer comprises the following steps：
1) cetostearyl alcohol/cetearyl glucoside, stearine/PEG-100 stearates, poly dimethyl silicon are weighed
Oxygen alkane, methyl hydroxybenzoate, propylben and ethylhexyl palmitate are added in 100mL beakers, are warming up to 80 DEG C -85 DEG C,
Stirring and dissolving, obtains solution A；
2) 200mL beakers are selected, first disperse carbomer and xanthan gum with butanediol, EDETATE SODIUM, allantoin, the third two are then added
Alcohol and deionized water, are warming up to 80 DEG C -85 DEG C, stirring fully dissolving obtains solution B；
3) by step 1) solution A that obtains is poured slowly into step 2) in the solution B that obtains, emulsifying homogeneous, homogeneous 6min, rotating speed is
3000r/min；
4) step 3) after the completion of homogeneous, emulsion is stirred and cooled, 60 DEG C of addition triethanolamines are down to, are stirred；
5) step 4) be cooled to 40 DEG C add aloe gels, hyaluronic acid, the antiallergy plant composition described in claim 1,
Sensiva SC50, stimulation inhibiting factor, avenabeta glucosan, are stirred, and PH to 5.5-6.5, cooling are adjusted with triethanolamine
Afterwards in canned to sterile Packaging Bottle.
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| CN107049866B (en) | 2020-05-08 |
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